J BUON [JOURNAL]
Sun
200 papers
Microsatellite instability (MSI) markers in thyroid carcinoma.
Mastronikolis N, Chrysovergis A, Tsiambas E … +1 more , Kyrodimos E
J BUON · 2021 · PMID 34268987
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Patient perspective on sequence order of anthracyclines and taxanes in neoadjuvant chemotherapy in HER2-negative breast cancer patients.
Altundag K
J BUON · 2021 · PMID 34268986
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Clinico-laboratory pertinences and management of relapsed and refractory.
Musteata V
J BUON · 2021 · PMID 34268985
PURPOSE: The purpose of this study was to assess the biological significance of lactate dehydrogenase (LDH), T315I mutation and treatment options in newly diagnosed and relapsed patients with chronic myeloid leukemia (CM...
PURPOSE: The purpose of this study was to assess the biological significance of lactate dehydrogenase (LDH), T315I mutation and treatment options in newly diagnosed and relapsed patients with chronic myeloid leukemia (CML). METHODS: Our clinical-analytical and descriptive study enrolled 27 patients with different phases of CML, who were followed up and treated at the Institute of Oncology between 1995-2020. Venous blood samples were taken for LDH measurement, molecular screening and detection of T315I mutation of the ABL gene in order to investigate the biological significance of the increased LDH values and T315I mutation. CML patients underwent chemotherapy with alkylating agents, antimetabolites and tyrosine kinase inhibitors (TKIs). RESULTS: The patient age ranged from 20 to 67 years (mean 51.3±2,14). The diagnosis of CML was established in the late chronic phase in 25 (92.6%) patients. The quantitative real-time PCR revealed p210 transcript of the BCR-ABL chimeric gene in all cases, with the range of 23.17-100% and median value of 74.73±3.21%. LDH at diagnosis ranged between 169-1609.4 U/L and was increased in 14 (63.6%) patients, especially in those with leukocytosis over 100x109/l. The complete cytogenetic and complete or major molecular responses were recorded under treatment with different generations of TKIs in 16 (59.3%) cases, including 3 cases with T315I mutation. Relapses occurred in 10 (71.4%) patients with initially increased LDH values and in 5 of 6 patients with T315I mutation. One (3.7%) patient with T315I mutation evolved into the acute phase disease, and achieved the complete hematological response after treatment with ponatinib, a 3rd generation TKI. The survival of patients from the disease onset till the last monitoring visit ranged between 21 and 234.8 months (median 93.97±4.52). CONCLUSIONS: The increased LDH values may indicate the activity at diagnosis and relapse of CML. In our study T315I mutation of the ABL gene and the increased values of LDH were associated with a higher rate of relapses and resistance to imatinib. Notwithstanding the treatment line and in relapses TKIs improve considerably the survival and ECOG-WHO score of CML patients.
Antioxidant preconditioning attenuates liver ischemia/reperfusion injury after hepatectomy in swine.
G Paradellis D, D Panousopoulos GS, G Mathioulaki A … +5 more , E Papalois A, N Nomikos T, Theocharis S, A Gkiokas G, F Arkadopoulos N
J BUON · 2021 · PMID 34268984
PURPOSE: The purpose of this study was to evaluate whether antioxidant preconditioning with Deferoxamine can attenuate liver ischemia reperfusion injury associated with extended hepatectomy in swine. METHODS: Eighteen sw...
PURPOSE: The purpose of this study was to evaluate whether antioxidant preconditioning with Deferoxamine can attenuate liver ischemia reperfusion injury associated with extended hepatectomy in swine. METHODS: Eighteen swine were randomly assigned to two groups: Deferoxamine (DFO) and Surgery Only (SO). The animals in both groups were subjected to laparotomy, prolonged temporary occlusion of the right and middle hepatic pedicles and subsequent left hepatectomy. The DFO group received IV deferoxamine prior to induction of liver ischemia. Monitoring was performed for 6 h and samples (Protein carbonyls, Thiobarbituric acid reactive substances, Histology, ALT, AST, Lactic acid and WBC) were drawn at 0, 60 and 360 min. RESULTS: Protein carbonyls and Thiobarbituric acid reactive substances had significantly lower concentration and higher reduction rates in serum and liver tissue of the DFO group. The histological examination of liver tissue showed less inflammation and necrosis in the DFO group. Hepatic enzymes and lactic acid measurements showed higher reduction rate in the DFO group by the end of the experiment. CONCLUSIONS: This experimental study documents an early protective effect of deferoxamine administration in major hepatectomies against liver ischemia/reperfusion injury.
Tracking the risk factors associated with high-risk cSCC: A 10-year, Two-Institution, Greek study.
Dimonitsas E, Champsas G, Kakagia D … +12 more , Stavrianos S, Roussos S, Doryforou O, Neamonitou F, Soura E, Kostopoulos E, Christofidou E, Papadopulos O, Thymara I, Papadopulos NA, Stratigos A, Korkolopoulou P
J BUON · 2021 · PMID 34268983
PURPOSE: We sought to identify independent risk factors for positive sentinel lymph node biopsy (SLNB), local recurrence (LR), metastasis (M) and death caused by cutaneous squamous cell carcinoma (cSCC) (DCS) in high-ris...
PURPOSE: We sought to identify independent risk factors for positive sentinel lymph node biopsy (SLNB), local recurrence (LR), metastasis (M) and death caused by cutaneous squamous cell carcinoma (cSCC) (DCS) in high-risk cSCC patients. Moreover, we compared the Brigham and Women's Hospital (BWH) system with the previous used in Greece (based on tumor size) and proposed a new classification system. METHODS: 1,524 cSCC patients were enrolled between January 2004 and December 2014, from two medical institutions. Potential risk factors for SLNB (local recurrence/LR, metastasis/M, death caused by SCC/DCS) were analyzed by univariate and multivariate Cox logistic regression models. RESULTS: Of the included patients with a median follow-up of 60 months 107 developed local recurrence (7%) while 84 developed metastases (5.5%). Among 36 patients undergoing sentinel lymph node biopsy (SLNB), 25% showed a positive SLNB with a false-negative result (11%). On multivariate analysis, key prognostic factors for LR were tumor diameter ≥2 cm, poor differentiation, incomplete excision and perineural invasion and for M were high-risk tumor site, tumor diameter ≥2 cm, poor differentiation, invasion beyond subcutaneous tissue, incomplete excision, perineural invasion and recurrence. DCS seems to be affected by tumor diameter ≥ 2 cm, poor differentiation, invasion beyond subcutaneous tissue, incomplete excision, perineural invasion and recurrence independently. CONCLUSIONS: These suggest the determined role of tumor diameter of cSCCs. Harnessing knowledge and collecting the up-to-date data along the clinical journey of high-risk cSCC, the future looks bright (development of new clinical trials, adjuvant therapies and tumor staging with SLNB).
Effects of Colchicum baytopiorum leaf extract on cytotoxicity and cell death pathways in C-4 I and Vero cell lines.
Dagdeviren Ozsoylemez O, Ozcan G
J BUON · 2021 · PMID 34268982
PURPOSE: The present study aimed to research the cytotoxic effects of Colchicum baytopiorum extract on normal and cancerous cells and reveal the cell death mechanisms in cancerous cells triggered by this effect. METHODS:...
PURPOSE: The present study aimed to research the cytotoxic effects of Colchicum baytopiorum extract on normal and cancerous cells and reveal the cell death mechanisms in cancerous cells triggered by this effect. METHODS: Within this framework, the cells' index values obtained with an xCELLigence Real Time Cell Analysis DP device, selectivity index (SI), apoptotic index (AI) based on a DAPI application and time-related activities of caspase 3,7 and 9 with a spectrofluorometer were inspected. The expressions of apoptosis/autophagy/entosis/necroptosis/anoikis-related genes were researched with qRT-PCR. RESULTS: It was determined that C. baytopiorum extract had displayed a high selectivity [(SI)=4], increased AI (p<0.01) and activation of caspases 3,7 and 9 (p<0.05). It was observed that the mRNA expressions of Atg12, Atg16, Atg5, Atg7, bad, bak, bax, bcl-xL, Beclin1, caspase3, FLIP, Puma, LC3, mcl-1, Bit1, Rho, RIP1, ROCK and TRAF2 genes in C-4 I cells to which the plant extract was applied had increased significantly in comparison with the control group (FC≥1.5). A lowering was detected in the mRNA levels of IAP, SRC kinase and TNF. CONCLUSION: Consequently, it was revealed that the plant extract used had increased the gene expressions in the autophagic cell death pathway in C-4 I cells along with apoptosis and thus, it could be a promising candidate for cervix carcinoma treatment.
Psychological well-being in cancer outpatients during COVID-19.
Bafunno D, Romito F, Lagattolla F … +15 more , Delvino VA, Minoia C, Loseto G, Dellino M, Guarini A, Catino A, Montrone M, Longo V, Pizzutilo P, Galetta D, Giotta F, Latorre AC, Russo A, Lorusso V, Cormio C
J BUON · 2021 · PMID 34268981
PURPOSE: The psychological status of cancer outpatients receiving anti-neoplastic treatment during the lockdown in a Italian non-COVID Cancer Center, was been investigated with the following aims: to measure the levels o...
PURPOSE: The psychological status of cancer outpatients receiving anti-neoplastic treatment during the lockdown in a Italian non-COVID Cancer Center, was been investigated with the following aims: to measure the levels of post-traumatic stress symptoms, depression and anxiety; to compare patients with different cancer sites; to compare the anxiety and depression levels measured in this emergency period between cancer and non-cancer patients and between cancer patients before and after the emergency. METHODS: The following questionnaires were used: The Hospital Anxiety and Depression Scale (HADs) and the Impact of Event Scale-Revised (IES-R).Worries regarding the COVID-19 on patients' lives, socio-demographic and clinical details were collected using a brief structured questionnaire. RESULTS: One-hundred seventy-eight outpatients were enrolled. We found that 55% of patients were above the cut-off for HADS general scale and 23.7% had severe level of PTSD. The 68% of patients declared that their worries have increased during the pandemic especially for women. Patients with lung cancer have higher general distress compared with patients with breast cancer and lymphoma. The non cancer sample had values significantly higher both for the IES-R scales and for HADS Depression subscale. Finally, cancer patients who experienced the health emergency showed higher levels of anxiety than those measured 2 years ago. CONCLUSION: Cancer out-patients of the present sample have severe post-traumatic stress symptoms and psychological distress, those with lung cancer are at higher risk and may need special attention. Non-oncological subjects have higher depression levels than cancer patients.
Decrease of oncological patients' hospital visits during Covid-19 pandemic; the experience of a tertiary Romanian centre.
Crisan C, Cainap C, Deac A … +6 more , Havasi A, Balacescu O, Balacescu L, Bochis O, Vlad C, Cainap S
J BUON · 2021 · PMID 34268980
PURPOSE: The outbreak of COVID-19 pandemic has changed the provision of medical services worldwide. We assessed the impact of the pandemic on the oncological patients' visits to a tertiary cancer centre. METHODS: We anal...
PURPOSE: The outbreak of COVID-19 pandemic has changed the provision of medical services worldwide. We assessed the impact of the pandemic on the oncological patients' visits to a tertiary cancer centre. METHODS: We analysed registrations from the administrative data system of in- and outpatients in all of the departments of the Cluj-Napoca Oncology Institute, during March-October 2020, and compared to the same 7-month period of the previous year. RESULTS: The decrease during March-October 2020 was 40.2% for new referrals overall (with the most significant drop in April, of 80%), 52.5% for medical oncology inpatients, 39% for paediatric oncology department inpatients, 69% for radiotherapy inpatients, 34.9% for surgical interventions and 31% decrease of issued pathology reports. The decrease was less important for outpatients: only 10% for medical oncology outpatient department, 33% for radiotherapy and 27% for breast cancer unit outpatients. Imaging investigations were only slightly influenced by the pandemic (reduction of 5% for MRI scans, 19% for mammograms,whereas performed CT scans were even more after the outbreak of COVID-19). CONCLUSION: Our results show a decrease in the number of patients during the period after the outbreak of the COVID-19 pandemic, more for inpatients and less significant for outpatient departments, probably because of the internal circuits reorganization but also because of health care measures taken nationally and locally to limit the spread of the pandemic.
Expression of miR-214 in pancreatic cancer and its effect on the biological function of pancreatic cancer cells.
Deng S, Wang J, Xu J … +3 more , Li J, Zhang L, Jin Y
J BUON · 2021 · PMID 34268979
PURPOSE: To study the expression and biological function of microRNA 214 (miR-214) in pancreatic cancer. METHODS: 101 patients with pancreatic cancer who came from First People's Hospital of Yunnan Province from December...
PURPOSE: To study the expression and biological function of microRNA 214 (miR-214) in pancreatic cancer. METHODS: 101 patients with pancreatic cancer who came from First People's Hospital of Yunnan Province from December 2013 to December 2016 were selected. 101 pancreatic cancer tissues and 101 adjacent tissues were resected and collected. The miR-214 expression was detected by qRT-PCR. Then the pancreatic cancer cell line AsPC-1 and SW1990 were transfected. MTT assay was used to detect cell viability and flow cytometry was used to detect apoptosis. Transwell chamber assay was used to detect the invasion and migration of cells in vitro. The protein expressions of ING4 in AsPC-1 and SW1990 cells were detected by Western blot. RESULTS: The relative expression of miR-214 in pancreatic cancer was significantly higher than that in adjacent tissues (p<0.05). There was a statistically significant difference between the expression level of miR-214 and T stage of pancreatic cancer (p<0.05). The relative expression of ING4 protein in SW1990 cells of miR-214 mimics group was significantly lower than that of miR-214 control mimics group (p<0.05), and that in AsPC-1 cells of the miR-214 inhibitors group was significantly higher than that in the miR-214 control inhibitors group (p<0.05). CONCLUSION: In conclusion, the expression of miR-214 is highly expressed in pancreatic cancer tissues, and the down-regulation of ING4 protein expression can inhibit the proliferation, invasion and migration of pancreatic cancer cells, promote their apoptosis, and can be used as a new molecular target for the diagnosis and treatment of pancreatic cancer.
Late effects of osteosarcoma and its treatment in pediatric patients: A single-center experience.
Nurdan T, Hatice Mine C, Emel U … +8 more , Handan D, Gulsah T, Incesoy Ozdemir S, Omer K, Yasin Y, Yusuf Y, Basarir K, Gulsan Y
J BUON · 2021 · PMID 34268978
PURPOSE: The success of osteosarcoma treatment strategies improved survival rates. The need of diagnosing and managing adverse effects is increasing. We aimed to investigate the outcomes and late results of pediatric ost...
PURPOSE: The success of osteosarcoma treatment strategies improved survival rates. The need of diagnosing and managing adverse effects is increasing. We aimed to investigate the outcomes and late results of pediatric osteosarcoma treatment in the survivors. METHODS: Out of osteosarcoma patients (n=54), we assessed the long-term outcomes of survivors (n=39) diagnosed from 2002-2018. We compared the survivors' (n=39) health status (cardiac, renal, neurologic, psychiatric, physical limitations), pain, and psychosocial outcomes (education level, smoking history, and alcohol consumption, marital status, parenthood, health care services usage) with their siblings (n=77). The quality of life and overall survival of amputee and nonamputee survivors are also compared. We provided the retrospective data from the files and phone calls and used Kaplan Meier survival analysis, Ki-Kare, and t-test. Results The overall survival (OS) of children with osteosarcoma (n=54) who survived at 2 years and 5 years from the diagnosis was 90.7 and 77.8%, respectively. These patients achieved 2- year event-free survival (EFS) of 70.4% and 5-year EFS of 57.4%. Thirty-nine survivors of osteosarcoma were compared with 77 sibling controls. Osteosarcoma survivors were more likely than the sibling cohort to report adverse health status containing nephrotoxicity (5.1 vs 0%) (p=0.045), cardiotoxicity (10.3 vs 0%)(p<0.01), neurotoxicity (5.1 vs 1.7%) (p=0.045), activity limitations (64 vs 1.3%)(p<0.01) and pain (12.8 vs 0%) (p=0.002). Survivors' educational status (p=0.014), marital status (5.1 vs 32.5%)(p=0.001), employment (2.6 vs 28.6)(p < 0.001), parenthood (0 vs 29.9%)(p < 0.001) were negatively affected compared with the control group. The prevalence of smoking, alcohol use, psychiatric treatment, and deafness were similar. The amputees (n=9) had an OS rate of 55.6%, and the nonamputees (n=45) had 75.6%. We found similar quality of life results between them. CONCLUSION: Long-term survivors of pediatric osteosarcoma are at significant risk of chronic health conditions, physical limitations, and pain up to 16 years follow up. Follow-up clinics and clinical guidelines are required for the survivors of children with osteosarcoma.
Laparoscopic radical resection for large-volume renal carcinoma: via retroperitoneal or transperitoneal approach?
Yin H, Zhou Z, Liu Q … +1 more , Qiu F
J BUON · 2021 · PMID 34268977
PURPOSE: The aim of this study was to investigate the clinical efficacy and safety of laparoscopic radical resection through retroperitoneal and transperitoneal approaches in treating large-volume renal carcinoma. METHOD...
PURPOSE: The aim of this study was to investigate the clinical efficacy and safety of laparoscopic radical resection through retroperitoneal and transperitoneal approaches in treating large-volume renal carcinoma. METHODS: A total of 116 patients with large-volume (>7 cm) renal carcinoma underwent laparoscopic radical resection for renal carcinoma. Among them, 58 were treated through retroperitoneal approach (Retroperitoneal group), and 58 were treated through transperitoneal approach (Abdominal group). The levels of interleukin-6 (IL-6), IL-12 and IL-1β in the patients were compared before and after operation. Furthermore, the levels of tumor markers were explored, and the tumor recurrence and survival of the patients were followed up and recorded. RESULTS: Compared with those in Abdominal group, the patients in Retroperitoneal group had remarkably shorter operation time, time of renal artery occlusion, time of intestinal exhaust and length of hospital stay after operation as well as notably smaller intraoperative blood loss. The levels of IL-6, IL-12 and IL-1β were elevated after operation in both groups in comparison with those before operation. Besides, the concentrations of serum CA50, CA125 and CEA declined obviously after treatment in the two groups in contrast with those before treatment, while no statistically significant differences in the concentrations of serum CA50, CA125 and CEA were observed between the two groups after treatment. The follow-up results indicated that the average survival and progression-free survival were 18.3 months and 16.0 months, respectively, in Retroperitoneal group, and 19.1 months and 16.8 months, respectively, in Abdominal group. CONCLUSIONS: The retroperitoneal laparoscopic radical resection for large-volume renal carcinoma possesses exact therapeutic effects, and it has shorter operation time, less blood loss, fewer impacts on inflammatory responses in patients and higher safety than transperitoneal laparoscopic radical resection.
Is 3D laparoscopic off clamp simple enucleation a feasible alternative for clinical T1 renal tumors? Outcomes from a single center experience.
George Rahota R, Valean D, Dobrota F … +5 more , Andras I, Rahota AC, Maghiar TT, Coman I, Crisan N
J BUON · 2021 · PMID 34268976
PURPOSE: To evaluate the safety of 3D laparoscopic off clamp simple enucleation (SE) of kidney tumors versus standard laparoscopic on-clamp partial nephrectomy (PN) in terms of perioperative, oncological and functional o...
PURPOSE: To evaluate the safety of 3D laparoscopic off clamp simple enucleation (SE) of kidney tumors versus standard laparoscopic on-clamp partial nephrectomy (PN) in terms of perioperative, oncological and functional outcomes. METHODS: All patients that underwent 3D laparoscopic nephron sparing surgery (NSS) in our department for clinical T1 tumors between January 2019-September 2020 were included. Of the total of 84 patients, 38 (45.24%) underwent SE (SE group) and 46 (54.76%) PN (PN group). Perioperative data was collected and analyzed. Oncological outcomes were evaluated by the positive surgical margin (PSM) rate and follow-up at 6 months after surgery. RESULTS: Mean age, gender, tumor size, PADUA score and length of hospital stay were comparable between the two groups. Estimated intraoperative blood loss (284.21 ml vs 151.52 ml, p=0.0001) and hemoglobin drop (p=0.0001) were significantly lower for the PN group. Patients that underwent SE showed a better preservation of renal function (eGFR drop of 4.4 ml/min vs 1.78 ml/min, p=0.75). No significant differences were found regarding the PSM, although the PSM rate was lower in the SE group when compared with the PN group (2.63% vs 4.34%, p= 0.07). CONCLUSION: Off-clamp simple enucleation of renal masses is feasible by laparoscopic approach and has produced comparable oncologic outcomes with standard on-clamp partial nephrectomy, with an incremental advantage for the preservation of renal function.
Comparison of peripheral blood stem cell mobilization with filgrastim versus pegfilgrastim in lymphoma patients - single center experience.
Lipan L, Colita A, Stefan L … +6 more , Calugaroiu C, Serban C, Tomuleasa C, Pasca S, Colita A, Tanase A
J BUON · 2021 · PMID 34268975
PURPOSE: The purpose of this study was to evaluate mobilization outcomes with biosimilar pegfilgrastim versus filgrastim in association with chemotherapy as a mobilization strategy for lymphoma patients. METHODS: In the...
PURPOSE: The purpose of this study was to evaluate mobilization outcomes with biosimilar pegfilgrastim versus filgrastim in association with chemotherapy as a mobilization strategy for lymphoma patients. METHODS: In the current study we included 32 lymphoma patients that received mobilization therapy and PBSC harvesting at the Bone Marrow Transplantation Department of Fundeni Clinical Institute, Bucharest, Romania between January and December 2019. RESULTS: Pegfilgrastim had beneficial effect when compared to filgrastim in reducing grade IV neutropenia both in the univariate and multivariate logistic models. Additionally, similar efficacy, as mobilization rate, after both filgrastim and pegfilgrastim was observed and no differences were noted between the two groups considering the need for platelet or red blood cell support. CONCLUSION: The use of biosimilar pegfilgrastim is a viable alternative to filgrastim in PBSC mobilization for lymphoma patients.
Prediction of optimal cytogenetic responses at 6 and 12 months in patients with chronic myeloid leukemia in chronic phase treated with imatinib.
Milosevic V, Jovanovic MP, Bukumirić Z … +5 more , Djordjevic V, Jovanovic J, Fekete MD, Lekovic D, Bogdanovic A
J BUON · 2021 · PMID 34268974
PURPOSE: Imatinib mesylate transformed the treatment and paradigms of chronic myeloid leukemia. European LeukemiaNet (ELN) group has defined specific treatment milestones with an optimal outcome to be achieved in patient...
PURPOSE: Imatinib mesylate transformed the treatment and paradigms of chronic myeloid leukemia. European LeukemiaNet (ELN) group has defined specific treatment milestones with an optimal outcome to be achieved in patients. METHODS: In a retrospective cohort study, we evaluated the impact of clinical and biological variables on achieving an optimal response at 6 and 12 months according to ELN recommendations. We included 106 patients with chronic phase chronic myeloid leukemia (CML) with appropriate bone marrow aspirate and biopsy for immunohistochemistry. RESULTS: The number of white blood cells (WBC), the percentage of peripheral blast, the values of Sokal and ELTS scores and the percentage of Ki-67+ cells in the bone marrow predicted a complete cytogenetic response (CCyR) at 6 months, but only WBC and EUTOS score predicts CCyR at 12 months. We found that Sokal score below 0.775 was very sensitive to achieve of CCyR at 6 months (m) and that all patients with a value <0.550 achieved CCyR-6m. Patients with a low percentage of blast in the peripheral blood (≤1.5%) or in the bone marrow (≤5%) together with lower WBC (≤100×109/L) were likely to have significantly higher CCyR rates at 6 and 12 months respectively. Also, patients with a higher number of Ki67+ cells in the leukemic areas of the bone marrow had a significantly better outcome. Unfortunately, our investigation did not reveal that bone marrow fibrosis (MF grade), microvascular density, percentage of CD34+, CD61+ or PTCH1+ cells could have any effect on achievement of CCyR at 6 or 12 months. CONCLUSION: Our investigation has shown that only a few biological characteristics in patients with CML can predict the optimal treatment outcome after imatinib.
Thoracoscopic-laparoscopic Ivor-Lewis surgery vs. McKeown surgery in the treatment of thoracic middle-lower segment esophageal cancer.
Shi Y, Wang A, Yu S … +3 more , Fei X, Liu S, Liao J
J BUON · 2021 · PMID 34268973
PURPOSE: The purpose of this study was to compare the efficacy and safety of thoracoscopic-laparoscopic Ivor-Lewis surgery and McKeown surgery in the treatment of thoracic middle-lower segment esophageal cancer. METHODS:...
PURPOSE: The purpose of this study was to compare the efficacy and safety of thoracoscopic-laparoscopic Ivor-Lewis surgery and McKeown surgery in the treatment of thoracic middle-lower segment esophageal cancer. METHODS: The clinical data of 136 patients with thoracic middle-lower segment esophageal cancer were divided into Ivor-Lewis group (n=68) and McKeown group (n=68). The perioperative indexes and the levels of tumor markers were observed. The patients' long-term survival condition was recorded via follow-up. Finally, the long-term quality of life of patients with a survival time >3 years was compared between the two groups after operation using EORTC QLQ-C30 and EORTC QLQ-OES18. RESULTS: The operation time was significantly shorter in Ivor-Lewis group than that in McKeown group. The hospitalization expenses were obviously higher in Ivor-Lewis group than those in McKeown group. The incidence rate of anastomotic fistula, anastomotic stenosis and pulmonary infection was evidently lower in Ivor-Lewis group than that in McKeown group. Moreover, the levels of serum CYFRA21-1, CA125 and CEA evidently declined in both groups after treatment compared with those before treatment. The follow-up results revealed that the 3-year survival rate was 72.1% and 64.7%, respectively. The analysis results of postoperative 3-year quality of life manifested that no statistically significant difference was observed in each index in QLQ-C30 between the two groups, but the dysphagia and reflux scores in QLQ-EOS18 were remarkably superior in Ivor-Lewis group to those in McKeown group. CONCLUSIONS: In the treatment of thoracic middle-lower segment esophageal cancer, minimally-invasive Ivor-Lewis surgery has shorter operation time, better life quality, and fewer postoperative complications (pulmonary infection, anastomotic fistula and anastomotic stenosis) than minimally-invasive McKeown surgery, while the treatment expenses are higher.
HOTTIP is upregulated in esophageal cancer and triggers the drug resistance.
Li Y, Liu Y, Chen G … +4 more , Liu H, Wu Y, Liu J, Zhang Z
J BUON · 2021 · PMID 34268972
PURPOSE: To explore the role of HOTTIP in the development of esophageal cancer and the drug resistance. METHODS: Serum level of HOTTIP in esophageal cancer patients was detected by RT-PCR. After treatment with different...
PURPOSE: To explore the role of HOTTIP in the development of esophageal cancer and the drug resistance. METHODS: Serum level of HOTTIP in esophageal cancer patients was detected by RT-PCR. After treatment with different concentrations of Adriamycin (ADM) in Eca109 cells for 24 h, IC50 was measured by MTT assay. Subsequently, Eca109 cells were treated with 0, 0.2, 0.4 or 0.8 μg/ml ADM, respectively, followed by extraction of extracellular vesicles (EVs). HOTTIP level in EVs was detected. In addition, Eca109 cells were pre-treated with EVs for 48 h, and different concentrations of ADM for another 24 h. IC50, cell cycle determination and relative levels of HOTTIP and ABCG2 were examined. Pearson correlation test was conducted to assess the correlation between levels of HOTTIP and ABCG2. RESULTS: Serum level of HOTTIP was higher in esophageal cancer patients. IC50 in ADM-treated Eca109 cells for 24 h was 0.43±0.02 μg/ml. EVs1-4 were respectively isolated from Eca109 cells treated with 0, 0.2, 0.4 or 0.8 μg/ml ADM for 24 h, respectively. HOTTIP remained the highest level in EVs4 than the other three groups. Notably, IC50 was much higher in Eca109 cells incubated with EVs4 than those treated with EVs1-3. EVs4 intervention in Eca109 cells for 48 h markedly increased the proliferation index (PI), and relative levels of HOTTIP and ABCG2 than the other three groups. HOTTIP displayed a positive correlation with ABCG2 in Eca109 cells. CONCLUSIONS: HOTTIP is highly expressed in serum of esophageal cancer patients. EVs-containing HOTTIP regulates drug resistance in esophageal cancer by positively activating ABCG2.
Association of obesity and other anthropometric characteristics with bladder cancer risk: a systematic review and meta-analysis of longitudinal cohort studies.
Tzelves L, Xenou D, Skolarikos A … +6 more , Varkarakis I, Deliveliotis C, Terpos E, Stamatelopoulos K, Sergentanis TN, Psaltopoulou T
J BUON · 2021 · PMID 34268971
PURPOSE: The purpose of this systematic review and meta-analysis was to evaluate the potential associations between anthropometric characteristics and bladder cancer risk, synthesizing longitudinal cohort studies. METHOD...
PURPOSE: The purpose of this systematic review and meta-analysis was to evaluate the potential associations between anthropometric characteristics and bladder cancer risk, synthesizing longitudinal cohort studies. METHODS: Literature search across MEDLINE, EMBASE, Scopus, Google Scholar and Cochrane Central was performed up to December 31, 2019 and data abstraction was performed independently by two authors. Random-effects (DerSimonian-Laird) models were used to estimate pooled relative risks (RR) and 95% confidence intervals (95%CI); subgroup analyses were performed in geographical region, mean age, publication year, length of follow-up, sample size, method of body mass index (BMI) estimation and adjustment for smoking. RESULTS: 27 studies were included (88 593 bladder cancer cases in a total cohort of 49 647 098 subjects). Increased bladder cancer risk was noted in overweight men (pooled RR=1.12, 95%CI: 1.04-1.21) but not in overweight women. Both obese men (pooled RR=1.14, 95%CI: 1.06-1.22) and women (pooled RR=1.19, 95%CI: 1.02-1.38) showed increased risk. Interestingly, height increase per 5 cm did not seem to affect risk of bladder cancer in men (pooled RR=1.03, 95%CI: 0.99- 1.06) and women (pooled RR=1.02, 95%CI: 0.97-1.06). Larger waist circumference was associated with bladder cancer risk in men (pooled RR=1.18, 95%CI: 1.09-1.26) but not women. CONCLUSION: Bladder cancer risk seems to be related with obesity overall and central obesity in men. In contrast to other cancer types, height does not seem to affect risk, but more studies are needed to extract safe conclusions.
The relation between tissue galectin-3 level and platinum resistance in neoadjuvant bladder cancer treatment.
Gokyer A, Kucukarda A, Kostek O … +6 more , Sayin S, Can N, Hacıoglu B, Erdogan B, Uzunoglu S, Cicin I
J BUON · 2021 · PMID 34268970
PURPOSE: This study aimed to reveal the relationship between the level of galectin-3 expression and the depth of response to neoadjuvant therapy in bladder tumor tissue with muscle invasion revealed by transurethral biop...
PURPOSE: This study aimed to reveal the relationship between the level of galectin-3 expression and the depth of response to neoadjuvant therapy in bladder tumor tissue with muscle invasion revealed by transurethral biopsy. METHODS: The percentage of galectin-3 staining in transurethral biopsy tissue with muscle invasion was determined by immunohistochemistry. The patients were divided into two groups: the down-staging (+) group consisting of patients with pathological complete response or non-invasive bladder cancer, and the down-staging (-) group consisting of patients with stage 2 and above. RESULTS: There were 11 patients in the down-staging (+) group and 12 patients in the down-staging (-) group. There was no significant difference between the two groups in terms of median age, gender, smoking, clinical stage at the time of diagnosis, distribution of carboplatin or cisplatin used as a platinum agent. Galectin-3 was positive in 2 patients (18.2%) in the group where down-staging was achieved with neoadjuvant therapy, while it was positive in 9 patients (75%) in the other group (p = 0.01). The median follow-up period of the patients was 31.6 months (95% CI 25.1-39.3). Overall survival was 43.4 months in the down-staging (+) group (95% CI 25.1-61.6) and 31.6 months in the down-staging (-) group (95% CI 12.7-50.6). Although there was a numerical difference, it did not reach statistical significance (p=0.37). CONCLUSION: The rate of down-staging after platinum-based neoadjuvant chemotherapy is significantly higher in patients with low galectin-3 staining in transurethral bladder biopsy tissue.
Efficacy of 131I therapy and its influencing factors in children and adolescents with differentiated thyroid carcinoma.
Luo M, Hu Y
J BUON · 2021 · PMID 34268969
PURPOSE: We aimed to explore the efficacy and safety of iodine-131 (131I) therapy in children and adolescents with differentiated thyroid carcinoma (DTC), and to analyze the possible factors affecting the efficacy. METHO...
PURPOSE: We aimed to explore the efficacy and safety of iodine-131 (131I) therapy in children and adolescents with differentiated thyroid carcinoma (DTC), and to analyze the possible factors affecting the efficacy. METHODS: A retrospective analysis was performed on the clinical data of 94 children and adolescent DTC patients aged ≤21 years old, who received 131I therapy. The efficacy was assessed, and the levels of serum thyroglobulin (Tg) and Tg antibody (TGAb), and adverse reactions were observed. Besides, the possible influencing factors for the efficacy were explored. RESULTS: There were 79 cases of overall response (84.0%) and 15 cases of no response (16.0%). Besides, the initial 131I ablation of thyroid remnants was efficacious in 61 (64.9%) patients, but not in 33 (35.1%) patients who continued to receive the subsequent 131I therapy. As of June 2019, 18 (54.5%) patients reached remission, whereas the other 15 (45.5%) patients failed to reach remission. Of 23 patients with distant metastases in the lungs, there were 8 cases of lesion elimination, 12 cases of improvement or stable disease and 3 cases of no response or progressive disease after 131I therapy. Among 44 patients with only lymph node metastasis, 28 patients were negative for the whole-body 18F-FDG tumor imaging. Moreover, the whole-body 18F-FDG tumor imaging was positive in 16 patients. Furthermore, the clinical tumor stage and pre-treatment Tg and RAIU were found to be the independent influencing factors for the efficacy of 131I therapy. CONCLUSION: 131I therapy is exactly effictive in treating DTC in children and adolescents, and the clinical tumor stage and pre-treatment Tg and RAIU are the major factors affecting the efficacy.
Clinical analysis of total endoscopic thyroidectomy via breast areola approach in early differentiated thyroid cancer.
Qu Y, Han Y, Wang W … +2 more , Zhang X, Ma G
J BUON · 2021 · PMID 34268968
PURPOSE: The purpose of this study was to explore the efficacy and safety of total endoscopic thyroidectomy (TET) via breast areola approach in the treatment of early differentiated thyroid cancer. METHODS: The clinical...
PURPOSE: The purpose of this study was to explore the efficacy and safety of total endoscopic thyroidectomy (TET) via breast areola approach in the treatment of early differentiated thyroid cancer. METHODS: The clinical data of 134 patients with early differentiated thyroid cancer were retrospectively analyzed. The patients underwent different treatments, including TET via breast areola approach in endoscope group (n=67), and conventional small incision open surgery in control group (n=67). The surgery-related indexes, complications, postoperative incision recovery, visual analogue scale (VAS) pain score, postoperative patients' satisfaction, tumor recurrence and survival conditions were compared between the two groups. RESULTS: Compared with control group, the endoscope group showed significantly longer operation time, smaller intraoperative bleeding, less postoperative drainage, shorter duration of postoperative catheter indwelling and shorter postoperative length of stay. Meanwhile, in the endoscope group, the postoperative VAS pain score was markedly lower than that in control group, and the postoperative patients' satisfaction was higher than that in control group. The neurological severity score (NSS) had statistically significant differences between the two groups at 3 months and 6 months after operation. Moreover, no tumor recurrence and metastasis were found during the follow-up period. CONCLUSIONS: TET via breast areola approach is safe and effective in the treatment of early differentiated thyroid cancer, and it can achieve a better cosmetic effect and high satisfaction of patients, which is worthy of clinical application.
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