Colloids Surf B Biointerfaces [JOURNAL]
Sun
200 papers
Drug-loaded nanomicelles with ROS-responsive controlled release of carnosic acid for the treatment of ulcerative colitis.
Zhang J, Zhang R, Wang E … +1 more , Zhao Y
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42401122 · Publisher ↗
Conventional mode of administration has limited efficacy in the treatment of ulcerative colitis (UC). Drug-loaded nanomicelles with smart responsive improve the bioavailability of the loaded drug, thereby improving thera...
Conventional mode of administration has limited efficacy in the treatment of ulcerative colitis (UC). Drug-loaded nanomicelles with smart responsive improve the bioavailability of the loaded drug, thereby improving therapeutic effects in UC. Herein, drug-loaded nanomicelles (VTH@CA) were formed via the self-assembly of the reactive oxygen species (ROS)-responsive thioketal (TK) bridging hydrophilic and hydrophobic groups, in which carnosic acid (CA) was encapsulated in the core of micelles. VTH@CA showed a well-dispersed spherical structure with nanoscale dimensions. The designed nanomicelles demonstrated excellent antibacterial, antioxidant and biocompatibility activities. Cellular experiments also confirmed that VTH@CA could effectively alleviate the inflammatory response of LPS-activated macrophages. Moreover, hyaluronic acid (HA) as the hydrophilic end, had specifically targeted cluster of differentiation 44 receptor, and subsequently the TK bond responded to ROS for triggered release of CA. Compared to free CA administration, VTH@CA significantly alleviated histopathological features in the colon, reduced the expression of pro-inflammatory cytokines, and suppressed cellular apoptosis, demonstrating superior therapeutic efficacy. Therefore, VTH@CA can achieve on-demand drug release and offer a promising therapeutic drug-loaded platform for the treatment of intestinal tract diseases.
Manganese‑containing mesoporous bioactive glass with antioxidative and osteogenic activities for periodontitis treatment.
Hao Y, Wang Z, Li X … +7 more , Su W, Liu Y, Hu Y, Fu J, Wang Y, Mei L, Xu Q
Colloids Surf B Biointerfaces · 2026 Jul · PMID 42398361 · Publisher ↗
Alveolar bone regeneration in periodontitis is a major clinical challenge due to high levels of reactive oxygen species (ROS). Mesoporous bioactive glass (MBG) is widely used for bone regeneration but lacks ROS scavengin...
Alveolar bone regeneration in periodontitis is a major clinical challenge due to high levels of reactive oxygen species (ROS). Mesoporous bioactive glass (MBG) is widely used for bone regeneration but lacks ROS scavenging capacity. Manganese (Mn) possesses cascade superoxide dismutase (SOD)/catalase (CAT)-mimetic activity. Herein, manganese-containing mesoporous bioactive glass (Mn-MBG) nanoparticles (∼70 nm) were synthesized via a sol-gel method. The Mn‑MBG released Mn, Si, and Ca ions and exhibited a ROS-scavenging effect through its SOD/CAT-mimetic cascade. In vitro, a low concentration of Mn-MBG showed good cytocompatibility, reduced intracellular and mitochondrial ROS levels, and restored mitochondrial membrane potential in human periodontal ligament stem cells (PDLSCs). It also regulated autophagy‑related protein expression (upregulating LC3‑II and reducing p62) and promoted osteogenic capacity under oxidative stress. In a rat periodontitis model, local administration of Mn-MBG significantly promoted alveolar bone regeneration without adverse effects. Collectively, these findings suggest that Mn-MBG alleviates oxidative stress and promotes bone regeneration, offering a potential strategy for periodontitis treatment.
Biomimetic PRMT1 inhibitor-loaded manganese-containing bimetallic MOF enhances NSCLC immunotherapy via cGAS-STING activation and PD-L1 blockade.
Feng S, Lv G, Zhang R … +3 more , Wang R, Cui Y, Dong N
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42398360 · Publisher ↗
Non-small cell lung cancer (NSCLC) remains one of the leading causes of cancer-related mortality worldwide, and its response to immune checkpoint blockade is frequently limited by an immunosuppressive tumor microenvironm...
Non-small cell lung cancer (NSCLC) remains one of the leading causes of cancer-related mortality worldwide, and its response to immune checkpoint blockade is frequently limited by an immunosuppressive tumor microenvironment and insufficient innate immune activation. Here, we developed a biomimetic manganese-containing bimetallic metal-organic framework (MOF) nanosystem, termed PMOFM, for enhanced NSCLC immunotherapy through PRMT1 inhibition, cGAS-STING activation, and PD-L1 blockade. PMOFM was constructed by loading a PRMT1-selective inhibitor into a manganese-containing bimetallic MOF and coating the nanoparticle with an anti-PD-L1-conjugated macrophage membrane to confer tumor-targeting and immunoregulatory properties. PMOFM exhibited favorable physicochemical characteristics, colloidal stability, efficient drug loading, and enhanced tumor accumulation. Mechanistically, PMOFM relieved PRMT1-mediated suppression of cGAS, while Mn⁺ release further enhanced cGAMP-STING signaling, resulting in increased cGAMP production, elevated cGAS and pSTING expression, and amplified downstream inflammatory responses. In both subcutaneous and orthotopic NSCLC mouse models, PMOFM achieved superior tumor suppression and significantly prolonged survival without evident systemic toxicity. Moreover, PMOFM markedly increased intratumoral IFN-β, CXCL10, TNF-α, IL-6, and IFN-γ levels and promoted CD4⁺ and CD8⁺ T-cell infiltration. Collectively, this study presents a biomimetic MOF-based nanoplatform that integrates innate immune priming with immune checkpoint blockade, providing a promising strategy for enhancing immunotherapy against NSCLC.
Interfacial engineering in lipase-catalyzed synthesis of functional lipids: Mechanisms, strategies, and prospects.
Ji S, Zhang J, Zhang J … +7 more , Chen X, Wu J, Zhang S, Yang P, Zou F, Ying H, Zhuang W
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42398359 · Publisher ↗
The regulation of multiphase interfacial reactions is a central challenge in heterogeneous catalysis, where interfacial physicochemical properties govern reaction efficiency and selectivity. Lipase-catalyzed lipid transf...
The regulation of multiphase interfacial reactions is a central challenge in heterogeneous catalysis, where interfacial physicochemical properties govern reaction efficiency and selectivity. Lipase-catalyzed lipid transformations represent a prototypical model for biocatalysis at soft interfaces. Lipase activity is intrinsically dependent on lipid-water interfaces, where interfacial activation via lid-domain rearrangement and dynamic redistribution of enzymes, substrates, and products introduce regulatory complexity beyond homogeneous enzymatic systems. Especially in functional and structured lipid synthesis, catalytic performance is often limited by interface-induced deactivation, mass transfer constraints, and side reactions such as acyl migration. This review summarizes recent advances in interfacial regulation strategies, focusing on how interfacial microenvironments govern lipase catalytic behavior, providing mechanistic insights for the rational design of advanced lipase-based catalytic systems.
Electroactive collagen nanofibrous scaffolds stabilized with polyphenols, dopamine, and reduced graphene oxide for infection-resistant bone regeneration.
González L, Fernández Y, Perez-Puyana VM … +5 more , Ostos FJ, Benhnia M, Bucio E, Fernández K, Romero A
Colloids Surf B Biointerfaces · 2026 Jul · PMID 42398358 · Publisher ↗
Electrospun scaffolds intended for post-resection bone reconstruction in osteosarcoma must combine ECM-mimetic architecture with sustained resistance to infection, oxidative stress, and premature functional depletion. He...
Electrospun scaffolds intended for post-resection bone reconstruction in osteosarcoma must combine ECM-mimetic architecture with sustained resistance to infection, oxidative stress, and premature functional depletion. Here, collagen/poly(ε-caprolactone) (COL/PCL) nanofibrous mats were functionalized with condensed tannins (10-20 wt%) and dopamine-functionalized reduced graphene oxide (rGO) to generate antioxidant, antibacterial, and electroactive interfaces. All formulations produced continuous bead-free fibers, while tannins modestly increased diameter and polydispersity and rGO induced a larger diameter shift and broader distributions. Dope rheology revealed enhanced shear-thinning behavior and increased consistency upon tannin addition, further reinforced by rGO, consistent with supramolecular structuring and nanofiller-mediated network formation. Tannins markedly improved wettability (water contact angle down to 59 °) and boosted antioxidant capacity (ORAC/ABTS/DPPH), whereas dopamine-rGO further increased ORAC/DPPH while suppressing tannin burst release and lowering cumulative polyphenol release, supporting interfacial immobilization and barrier-controlled transport. Degradation in PBS and collagenase was significantly slowed by tannins and further stabilized by dopamine-rGO, with kinetic fitting indicating multi-regime behavior and reduced early/late-stage erosion rates. Electrochemical impedance spectroscopy showed reduced impedance with rGO, and apparent conductivity was recovered to near-control levels. Biological validation demonstrated near-complete antibacterial activity against E. coli and S. aureus (up to 99%), low hemolysis (1-1.6%), and selective reduction of U2OS osteosarcoma viability in dopamine-rGO scaffolds while maintaining high viability in Jurkat, U937, and HeLa cells. These results position dopamine-rGO/tannin-functionalized COL/PCL scaffolds as promising multifunctional platforms for infection-resistant, electroactive, and structurally stable bone regeneration in orthopedic oncology applications.
Unmodified orientable osteoclastic cytomembrane bionic fluorescent magnetic nanocarbons as high-efficiency multifunctional platforms for antiresorptive compound discovery.
Zhao Q, Han L, Zhang C … +5 more , Liu S, Yu X, Yu S, Zhang Q, Qin L
Colloids Surf B Biointerfaces · 2026 Jul · PMID 42398357 · Publisher ↗
As a valuable source for novel drug discovery, traditional herbal medicines are vital in treating diverse diseases. Pathological cell membrane (CM)-camouflaged nanomaterials have been validated as a robust tool for bioac...
As a valuable source for novel drug discovery, traditional herbal medicines are vital in treating diverse diseases. Pathological cell membrane (CM)-camouflaged nanomaterials have been validated as a robust tool for bioactive component screening. The growing demand for precise, rapid drug screening further requires the targeted and in-situ identification of bioactive compounds. Herein, novel inside-out osteoclast cytomembrane (OC) directionally cloaked fluorescent magnetic carbonaceous nanospheres (OCD@FMCNs) were elaborately developed and utilized as a multifunctional nanoplatform for high-efficiency discovery of natural anti-osteoporosis ingredients. Fluorescein isothiocyanate-wheat germ agglutinin (FITC-WGA) were bonded onto magnetic carbonaceous nanospheres (MCNs) by facile amidation reaction, which further specifically integrated with the glycosylated extracellular surface of OC to form OCD@FMCNs. The introduction of FITC-WGA boned MCNs (FWGA-MCNs) ensured the enhanced stability, favorable magnetism, more binding sites, fluorescence property and complete extracellular surface exposure of OCD@FMCNs without extra labelling of cytomembrane. The mixture of five bioactive compounds was extracted from Phellodendron chinense Schneid. by OCD@FMCNs and preliminarily affirmed by rapid osteoclast imaging tests. A novel potential anti-resorptive component targeting the inside surface of OCs was further identified among them and systemically investigated by network pharmacology, in vitro and vivo experiments. This work provided a facile scalable strategy for constructing versatile platforms with both targeted drug screening and validation.
A synergistic nanoplatform integrating phototherapy-triggered immunogenic cell death and PKM2-targeted gene therapy for durable tumor control.
Sun Q, Ma Y, Peng M … +7 more , Lin M, Jin X, Yelebolati S, Yang Y, Wu S, Zhang M, Cui C
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42398356 · Publisher ↗
Cancer treatment continues to face challenges due to tumor heterogeneity, metastasis, and adaptive resistance. Phototherapy represents a promising non‑invasive approach that can achieve localized tumor ablation and syste...
Cancer treatment continues to face challenges due to tumor heterogeneity, metastasis, and adaptive resistance. Phototherapy represents a promising non‑invasive approach that can achieve localized tumor ablation and systemic immune activation through immunogenic cell death (ICD). However, its efficacy is hindered by the instability of photoactive agents such as indocyanine green (ICG) and by therapy‑induced resistance, particularly heat shock protein 70 (HSP70)‑mediated thermotolerance. To address these limitations, an integrated nanotherapeutic platform was developed based on polydopamine (PDA) co‑loaded with ICG and siRNA targeting pyruvate kinase M2 (siPKM2), and functionalized with polyethylenimine (PEI). The resulting PDA‑ICG@PEI/siPKM2 nanoplatform executes three functionally coordinated stages: (1) enhanced photothermal ablation for immediate tumor destruction, which triggers robust ICD; (2) ICD‑driven activation of systemic antitumor immunity; and (3) siPKM2‑mediated dual modulation of tumor metabolism and signaling-depleting glycolytic ATP to impair HSP70‑mediated thermotolerance while suppressing oncogenic pathways. Together, these mechanisms create a self‑reinforcing cycle in which phototherapy‑induced ICD primes an immune response that is further amplified and sustained by PKM2‑targeted gene therapy. This combinatory strategy synergistically enhances phototherapeutic efficacy and immune activation, offering a promising approach for tumor control.
A photothermally addressable Tomato extracellular vesicle-integrated polypyrrole/gellan gum hydrogel for microenvironmental reprogramming of diabetic wounds.
Chang YJ, Liou YW, Rethi L … +7 more , Chuang SC, Hung PC, Huang CC, Shu YT, Chang JF, Fuh CS, Chuang AE
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42391663 · Publisher ↗
Chronic diabetic wounds remain difficult to treat because they are characterized by persistent oxidative stress, prolonged inflammation, impaired angiogenesis, and delayed tissue regeneration. Here, we report a smart TEV...
Chronic diabetic wounds remain difficult to treat because they are characterized by persistent oxidative stress, prolonged inflammation, impaired angiogenesis, and delayed tissue regeneration. Here, we report a smart TEV/PVA-PEI-PPY@GG hydrogel that integrates tomato-derived extracellular vesicles (TEV), a polypyrrole (PPY)-based near-infrared (NIR)-responsive photothermal component, and a gellan gum (GG) matrix for diabetic wound treatment. The engineered platform exhibited favorable colloidal stability, a porous and structurally integrated architecture, tunable mild photothermal responsiveness under 808 nm irradiation, and retained antioxidant activity associated with the vesicular fraction. In vivo, TEV/PVA-PEI-PPY@GG significantly accelerated wound closure, improved epidermal continuity, and enhanced dermal remodeling in streptozotocin-induced diabetic wounds, while showing no obvious histopathological toxicity in major organs. Immunohistochemical and histological analyses revealed altered expression patterns of AHR, TNF-α, CD31, and CD34 in treated tissues, which may contribute to tissue repair, modulation of inflammatory responses, and angiogenic remodeling during wound healing. These changes were associated with improved wound regeneration and restoration of tissue architecture. Overall, this work demonstrates the potential of a plant EV-integrated photothermally responsive hydrogel as a therapeutic strategy for diabetic wound repair and supports the possibility that local microenvironment modulation may contribute to its beneficial effects.
Simultaneous disruption of glutathione and 7-dehydrocholesterol antioxidant armor drives lipid peroxidation cascade for robust ferroptotic tumor elimination.
Wang Z, Yang J, Ma H … +10 more , Que F, Cai H, Bai T, Chen D, Zhu J, Chen X, Fan Q, Huang L, Shen Z, Yu X
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42391662 · Publisher ↗
Ferroptosis therapy is fundamentally constrained by the robust and adaptive redox homeostasis networks of tumor cells. Notably, 7-dehydrocholesterol (7-DHC) has recently emerged as a formidable, non-canonical metabolic f...
Ferroptosis therapy is fundamentally constrained by the robust and adaptive redox homeostasis networks of tumor cells. Notably, 7-dehydrocholesterol (7-DHC) has recently emerged as a formidable, non-canonical metabolic firewall that directly intercepts lipid peroxyl radicals at the plasma membrane, rendering conventional glutathione (GSH)-depleting strategies insufficient. To overcome the dual-layered antioxidant defense, we developed a TME-responsive nanoreactor (TS/GLA@HMION) to orchestrate a synchronized "fuel-spark-breach" lipid peroxidation (LPO) cascade. The nanoreactor was constructed by co-encapsulating TASIN-1 (TS) and γ-linolenic acid (GLA) within hollow mesoporous iron oxide nanoparticles (HMION). Upon TME-triggered disassembly, the released GLA undergoes targeted metabolic incorporation via ACSL1, massively expanding the membrane-localized substrate pool for peroxidation to serve as the required fuel. The iron oxide framework functions as a continuous catalytic engine, driving a sustained Fenton-like reaction to generate abundant •OH as the essential catalytic spark. Meanwhile, TS-mediated inhibition of 7-DHC biosynthesis eliminates the terminal membrane-associated radical scavenging capacity. Coupling 7-DHC blockade with iron-mediated GSH depletion decisively breaches the cellular antioxidant shields. Consequently, the synergy of substrate enrichment, radical generation, and antioxidant disruption transforms restricted oxidative stress into the strong LPO cascade, presenting a potent metabolic redox paradigm for treating refractory malignancies.
An electrochemiluminescence biosensor based on the hairpin-mediated exponential amplification and CRISPR/Cas12a amplification for ultrasensitive detection of MMP-2.
Zheng C, Nong L, Luo J … +5 more , Liang L, Wei Y, Luo Q, Zhang K, Liao X
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42391661 · Publisher ↗
In this study, we report a novel electrochemiluminescence (ECL) biosensor for the ultrasensitive detection of matrix metalloproteinase-2 (MMP-2), an important biomarker associated with tumor invasion and metastasis. The...
In this study, we report a novel electrochemiluminescence (ECL) biosensor for the ultrasensitive detection of matrix metalloproteinase-2 (MMP-2), an important biomarker associated with tumor invasion and metastasis. The biosensor integrates hairpin-mediated exponential amplification with CRISPR/Cas12a-based trans-cleavage for dual-stage signal amplification. In this design, MMP-2 specifically cleaves a peptide sequence (GPLG↓VRGK) on the DNA hairpin probe (HP1), releasing an initiator peptide nucleic acid (PNA) that triggers hairpin-mediated exponential amplification reaction. The amplified DNA products then activate the Cas12a/gRNA complex, which induces collateral cleavage of ferrocene (Fc)-labeled probes immobilized on a DNA tetrahedron-modified PEI-Ti₃C₂Tx/Ru/AuNPs electrode, thereby generating a strong ECL response. The incorporation of the DNA tetrahedron nanostructure provides a well-defined three-dimensional framework that ensures ordered probe orientation, enhanced hybridization efficiency, and reduced steric hindrance on the electrode surface. This structural organization significantly improves electron transfer and signal stability compared with conventional planar immobilization. Under optimized conditions, the biosensor exhibited a broad linear range from 0.01 fM to 10 nM and an ultralow detection limit of 10 aM. It displayed high specificity against interfering proteins (thrombin, IgG, BSA, lysozyme), excellent stability, and satisfactory recoveries (96.9%-105.0%) in LO2 cell culture supernatants. Overall, this enzyme-responsive, DNA-tetrahedron-assisted, CRISPR-amplified ECL biosensor represents a robust and versatile platform for precise and rapid detection of protease activity, showing great promise for biomedical diagnostics and clinical biomarker monitoring.
Nose-to-brain delivery of riluzole-loaded nanoemulsion in a controlled cortical impact-induced traumatic brain injury: Insights fromIn vitro,Ex vivo, andIn vivostudies.
Amulya E, Kumari D, Kumar R … +3 more , Vambhurkar G, Dandekar MP, Srivastava S
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42391660 · Publisher ↗
Traumatic brain injury (TBI) triggers oxidative stress, neuroinflammation, and functional impairments, yet effective brain-targeting therapies remain limited. The current study reports the development and evaluation of a...
Traumatic brain injury (TBI) triggers oxidative stress, neuroinflammation, and functional impairments, yet effective brain-targeting therapies remain limited. The current study reports the development and evaluation of an intranasal (IN) riluzole-loaded mucoadhesive nanoemulsion (RLZ-MNE) to enhance nose-to-brain delivery and therapeutic efficacy in TBI. The optimized RLZ-MNE exhibited a mean globule size of 21.24 ± 0.5 nm and spherical morphology. The formulation showed nasal-compatible pH and excellent spreading behavior, as reflected by a reduced contact angle (30.691 ± 0.1 °). Ex vivo permeation studies depicted significantly enhanced flux (10.98 ± 0.79 µg/cm/h) and permeability coefficient (3.66 × 10 ± 0.0003 cm/h) compared to pristine drug, with histological evidence of preserved mucosal integrity. In vitro studies in HO-induced SH-SY5Y cells confirmed neuroprotection, evidenced by improved cell viability, reduced nitrosative stress and reactive oxygen species, and restoration of mitochondrial membrane potential. Pharmacokinetic evaluation revealed a ∼2.8- and ∼3.85-fold enhancement in brain C following RLZ-MNE IN rather than RLZ-IN and RLZ-IV, respectively. Additionally, enhanced N2B transport was confirmed by increased drug targeting efficiency (%DTE, ∼5.4-fold) and direct transport percentage (%DTP, ∼4.81-fold) compared to RLZ-IN. In vivo, IN administration of RLZ-MNE at high and low doses significantly reduced neurological severity scores (⁓6-fold and 7.6-fold vs. CCI), improved motor coordination in the rota-rod test (*p < 0.05), enhanced preference index (****p < 0.0001; **p < 0.01), and discrimination index (*p < 0.05) relative to the CCI group. RLZ-MNE markedly decreased brain water content (***p < 0.001), indicating attenuation of cerebral edema. The pro-inflammatory cytokines were also considerably reduced compared to the CCI. Immunohistochemical analysis demonstrated the downregulation of p-NF-κB expression, and RT-PCR results depicted the restoration of EAAT2 mRNA expression following treatment with RLZ-MNE. Histopathological evaluation further corroborated these findings by demonstrating reduced lesion severity and preservation of neuronal architecture in RLZ-MNE-treated groups. Overall, RLZ-MNE demonstrated superior nasal permeation, antioxidant capacity, and neuroprotective efficacy, highlighting its promise as an efficient IN brain delivery approach for the management of TBI.
Effect of ipratropium bromide on the interfacial organization of model lung surfactant membranes.
Marczak M, Fontaine P, Batys P … +1 more , Matyszewska D
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42385488 · Publisher ↗
The interactions of inhaled drugs with lung surfactant are important for understanding their behavior at the first biological barrier of the respiratory tract. In this work, we investigated the effect of the anticholiner...
The interactions of inhaled drugs with lung surfactant are important for understanding their behavior at the first biological barrier of the respiratory tract. In this work, we investigated the effect of the anticholinergic bronchodilator, ipratropium bromide (IPB), on model lung surfactant membranes composed of dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG), and their 8:2 molar mixture. Langmuir monolayer studies showed that ipratropium bromide has little influence on zwitterionic DPPC, while it interacts strongly with negatively charged DPPG, leading to concentration-dependent expansion of the monolayer, stabilization of the liquid-expanded phase, and a shift of the phase diagram toward higher surface pressures. For the mixed DPPC:DPPG 8:2 model, the effect of the drug was moderate and mainly visible at low surface pressures, where it fluidized the layer and delayed the development of condensed domains. Brewster angle microscopy confirmed these observations by revealing altered domain morphology for DPPC, extended phase coexistence and circular domains for DPPG, and reduced condensation of the mixed model in the presence of ipratropium at lower surface pressures. Compression-expansion experiments mimicking the breathing cycle demonstrated that IPB decreases the reversibility of the mixed monolayer and promotes aggregate formation. GIXD and XRR measurements showed that it does not alter the in-plane or vertical structure of DPPC, but significantly affects DPPG by inducing structural features characteristic of lower-pressure phases and slightly increasing electron density in the headgroup region. Molecular dynamics simulations confirmed preferential localization of ipratropium bromide near DPPG headgroups, driven mainly by electrostatic interactions.
Construction of a bio-mimetic outer membrane layer via autodisplay of scFv onE. colifor SPR-based amyloid-β detection.
Oh MJ, Lee J, Jose J … +2 more , Bong JH, Park M
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42385487 · Publisher ↗
The development of highly sensitive biointerfaces is essential for the early diagnosis of Alzheimer's disease, characterized by the accumulation of amyloid-β(Aβ). In this study, a functionalized outer membrane layer usin...
The development of highly sensitive biointerfaces is essential for the early diagnosis of Alzheimer's disease, characterized by the accumulation of amyloid-β(Aβ). In this study, a functionalized outer membrane layer using an scFv autodisplay system was constructed to enhance the performance of Surface Plasmon Resonance (SPR) biosensors. The single-chain variable fragment (scFv) against Aβ was genetically fused to an autotransporter and successfully expressed on the Escherichia coli outer membrane (OM). The biological activity and orientation of the autodisplayed scFv were validated via flow cytometry and fluorescence microscopy, showing a 35-fold higher binding affinity to Aβ compared to non-engineered controls. For the construction of the biointerface, isolated OM fractions were immobilized onto a gold substrate, forming a stable OM layer as confirmed by FT-IR spectroscopy. SPR analysis demonstrated that the scFv autodisplaying OM layer exhibited superior sensitivity with a limit of detection below 0.1 μg/mL and highly specific, concentration-dependent binding to Aβ. Crucially, the inherent physicochemical properties of the OM layer effectively minimized non-specific interactions, eliminating the need for additional blocking agents and ensuring structural stability even after washing steps. These findings establish the scFv autodisplay on an OM layer as a robust, scalable, and orientation-controlled platform for the label-free detection of biomarkers in complex biointerfaces.
Carbon dot crosslinked hydrogel with microenvironment modulation for wound healing via synergistic antibacterial, antioxidant and hypoxia mitigation.
Zhao D, Pan R, Lu G … +2 more , Xuan Y, Wang J
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42385486 · Publisher ↗
The development of wound dressings that synergistically hemostasis, anti-bacterial, anti-inflammatory, and hypoxia mitigation remains challenging. Herein, a multifunctional hydrogel dressing (DCCG) was fabricated using d...
The development of wound dressings that synergistically hemostasis, anti-bacterial, anti-inflammatory, and hypoxia mitigation remains challenging. Herein, a multifunctional hydrogel dressing (DCCG) was fabricated using dextran-derived aldehyde-rich carbon dots (Dex-CDs) and Cu to crosslink with carboxymethyl chitosan (CMCS) via Schiff base reaction and metal coordination interactions. The DCCG hydrogel with pH/NIR responsive allowed controlled release CDs and Cu to eliminate bacteria and destruct biofilms. Moreover, the DCCG hydrogel significantly promoted fibroblast migration, angiogenesis, and collagen deposition to accelerate wound healing. These beneficial effects can be attributed to the regulation of the local microenvironment: CDs efficiently scavenged reactive oxygen species (ROS) to alleviate oxidative stress, Cu facilitated oxygen generation to relieve hypoxia and promote neovascularization, thus inducing polarization of macrophages toward the pro-regenerative M2 phenotype to facilitate regeneration. This work offers a multifunctional and promising platform for next-generation intelligent wound management.
Modulation of membrane deformability and stability in liposomes incorporating arginine-based surfactants.
Hermet M, Fait ME, Sabatie AE … +4 more , Romero EL, Fanani ML, Morcelle SR, Bakas LS
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42385485 · Publisher ↗
This study investigates the ability of two arginine-based surfactants-N-benzoyl-arginine decyl- or dodecylamide hydrochloride (Bz-Arg-NHC or Bz-Arg-NHC, respectively)-to act as edge activators (EA) in soy phosphatidylcho...
This study investigates the ability of two arginine-based surfactants-N-benzoyl-arginine decyl- or dodecylamide hydrochloride (Bz-Arg-NHC or Bz-Arg-NHC, respectively)-to act as edge activators (EA) in soy phosphatidylcholine (SPC) liposomes. Liposomes containing 20 mol% of either Bz-Arg-NHC or Bz-Arg-NHC (SPC20C10 and SPC20C12, respectively), along with surfactant-free controls, were prepared by thin-film hydration and characterized in terms of size, polydispersity index, and Z potential. Vesicle deformability was assessed by extrusion-based assays, while membrane organization and interfacial hydration were evaluated using Laurdan generalized polarization (GP). Lateral packing properties were analyzed through surface pressure-area isotherms of the corresponding lipid monolayers, and storage stability was monitored over 90 days at 4 °C. Both surfactants were incorporated without significantly affecting vesicle size (∼140-145 nm). However, only SPC20C10 exhibited markedly enhanced deformability (D = 6358 % P·min⁻¹), whereas SPC20C12 showed values comparable to control liposomes. Laurdan analysis revealed decreased GP values for both systems, indicating increased interfacial disorder, while a significant reduction in compressibility modulus was observed only for SPC20C10, reflecting disruption of lateral packing. Stability studies showed that SPC20C10 preserved its physicochemical properties for up to 30 days but exhibited reduced long-term stability compared to controls. These results demonstrate that Bz-Arg-NHC acts as an effective EA by promoting membrane softening through disruption of lateral packing, whereas Bz-Arg-NHC induces interfacial changes without sufficient mechanical modulation. Overall, our work reveals that interfacial disorder is not sufficient to yield ultradeformability; instead, a measurable loss of lateral packing cohesion appears as the key mechanical signature of effective edge activation in SPC membranes.
Multifunctional MXene/PVDF nanofiber membrane with electroactivity and mild photothermal effect for guided bone regeneration.
Zhong Y, Lai Y, He D … +5 more , Lan KF, Ye S, Wu Y, Fu Y, Mo A
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42378774 · Publisher ↗
Bone defects have become a major challenge restricting the long-term success of dental implant restoration. Conventional barrier membranes often exhibit limited capability in guided bone regeneration, particularly in sev...
Bone defects have become a major challenge restricting the long-term success of dental implant restoration. Conventional barrier membranes often exhibit limited capability in guided bone regeneration, particularly in severe defect cases. To address these issues, a novel multifunctional MXene/PVDF nanofiber membrane was fabricated via electrospinning. First, the membrane possesses favorable mechanical and physical barrier properties, effectively preventing the invasion of soft tissue cells into the bone defect area while providing stable mechanical support. Second, the composite membrane is endowed with excellent "electroactive + mild photothermal" functionality. Through in vitro cellular assays and in vivo animal experiments, we have demonstrated that the membrane exhibits good biocompatibility and can promote bone tissue regeneration through the combined effects of electrical response and photothermal stimulation. Finally, the long-term chemical stability and safety of the MXene/PVDF nanofiber membrane have been preliminarily validated. In summary, the MXene/PVDF nanofiber membrane exhibits outstanding "barrier + electroactive + mild photothermal" performance and holds great potential for guided bone regeneration.
Electrophoretic deposition-assisted layer-by-layer construction of carbon nanotube-MnO-based hybrid biofilm for supercapacitive microbial fuel cells.
Chen S, Li Y, Wang S … +5 more , Peng X, Sun J, Zhang L, Song RB, Li Z
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42378773 · Publisher ↗
The exploitation of hybrid biofilm with alternating functional layer represents a promising way to upgrade the performance of supercapacitive microbial fuel cells (SC-MFCs). However, the popularization of this special hy...
The exploitation of hybrid biofilm with alternating functional layer represents a promising way to upgrade the performance of supercapacitive microbial fuel cells (SC-MFCs). However, the popularization of this special hybrid biofilm requires a convenient and scalable construction strategy. Herein, the electrophoretic deposition-assisted layer-by-layer construction strategy has been developed to successively deposit carbon nanotube-manganese dioxide nanocomposite (C-M) and bacterial cells for the formation of hybrid biofilm. When using in SC-MFCs, the hybrid biofilm with two repetitive C-M/bacteria bilayers displayed the optimal performance due to the improvement of functional components without the sacrifice of electron transfer efficiency and bacterial metabolic activity. Moreover, the intercalation of capacitive and conductive C-M layer between bacteria layers enlarges their interaction interface and builds an accelerated electron transfer network inside the developed hybrid biofilm. As a result, the developed hybrid biofilm displayed enhanced capacitance, improved electricity generation capacity and promoted self-charge-discharge behavior compared to the counterpart biofilms. This work demonstrates the potential of electrophoretic deposition technology for the alternative construction of different functional layers in hybrid biofilm, which will be attractive for SC-MFCs and other microbial electrochemical systems.
Near-infrared light-promoted multimodal synergistic targeted therapy for liver cancer based on copper-gold nanospheres-graphene nanocomposites.
Wang Y, Wang Y, Tian J … +4 more , Shi M, Gong T, Guo R, Wang N
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42378772 · Publisher ↗
Liver cancer is one of the most prevalent malignant tumors worldwide that poses significant challenges to conventional treatment due to issues such as poor biocompatibility, inadequate targeting, and the limited efficacy...
Liver cancer is one of the most prevalent malignant tumors worldwide that poses significant challenges to conventional treatment due to issues such as poor biocompatibility, inadequate targeting, and the limited efficacy of single-agent therapies. These limitations can reduce treatment effectiveness and make accurate antitumor therapy more difficult. In recent years, the rapid development of multifunctional nanotechnology have substantially improved liver cancer treatment. This study designed folic acid (FA) and triformylcholic acid (TCA) targeted nanocomposites (GO-AuNSs-FA-TCA@BSA-Cu, GSFT@BCu) for liver cancer. This nanosystem integrates photothermal therapy (PTT), photodynamic therapy (PDT) and chemodynamic therapy (CDT) to construct a multimodal therapeutic platform, and introducing cuproptosis and apoptosis as a novel approach for precise modulation of tumor cell death. Transmission electron microscopy (TEM) and ultraviolet-visible (UV-Vis) spectroscopy were employed to characterize the morphological features and optical properties of the as-prepared nanocomposites. In vitro experiments confirmed that GSFT@BCu possessed a high photothermal conversion efficiency of 27.26% and can release copper ions in an NIR/GSH-responsive manner, further inducing oxidative stress and changes in mitochondrial membrane potential. In vivo liver cancer models showed that this combined multimodal therapy yielded a tumor inhibition rate of 82.47%. Additionally, the nanocomposite displayed outstanding blood compatibility and biosafety toward normal tissues. Overall, GSFT@BCu demonstrates excellent biocompatibility and prominent synergistic therapeutic effects against liver cancer, which providing a critical nanoplatform for multimodal synergistic therapy and precision medicine in oncology.
Antimicrobial peptide hydrogel delivery systems: Design strategies and functional expansion.
Zhao Y, Tu Y, Zheng S … +3 more , Li S, Shao C, Chen P
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42372463 · Publisher ↗
Antimicrobial peptides (AMPs), owing to their unique membrane-disrupting mechanism and low susceptibility to resistance, represent important candidate therapeutics for combating multidrug-resistant bacterial infections....
Antimicrobial peptides (AMPs), owing to their unique membrane-disrupting mechanism and low susceptibility to resistance, represent important candidate therapeutics for combating multidrug-resistant bacterial infections. However, their clinical application is constrained by issues such as poor in vivo stability, short half-life, low bioavailability, and potential toxicity. Hydrogels, as biocompatible delivery platforms, can effectively encapsulate, protect, and control the release of AMPs through their three-dimensional network structure, significantly improving their pharmacokinetic properties and therapeutic efficacy. This review outlines the principles underlying the construction of peptide-based hydrogels, hybrid material hydrogels, composite delivery systems, and stimulus-responsive hydrogels, with particular emphasis on how these design strategies specifically address the challenges associated with delivering AMPs. Building upon this foundation, we have conducted an in-depth analysis of characteristics and existing challenges of hydrogels containing AMPs through three key pathways, including synergistic antimicrobial action, microenvironment regulation, and optimisation of in vivo processes. The article concludes by outlining the immense potential of artificial intelligence technology in advancing the intelligent design and personalised treatment of hydrogels containing AMPs. It further examines the core challenges currently confronting clinical translation and explores future development directions. This work aims to provide theoretical reference and practical guidance for the research and development of next-generation high-performance hydrogel systems containing AMPs.
Ultrasmall pH-triggered cerium-based MOF nanozyme delivery system: Stable implant coating with integrated synergistic antibacterial and corrosion resistance enhancement.
Cui J, Wu S, Ye J … +3 more , Cao P, Wang Z, Liu X
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42372462 · Publisher ↗
The attachment of bacteria and subsequent biofilm formation is the first step toward the colonization of medical devices and implants by bacteria. Preventing the colonization of bacteria is key to averting infections. At...
The attachment of bacteria and subsequent biofilm formation is the first step toward the colonization of medical devices and implants by bacteria. Preventing the colonization of bacteria is key to averting infections. At the same time, the bacterial type Staphylococcus aureus (S.aureus) is the main pathology affecting infections on implant surfaces. The joint effects of natural selection and antibiotics have resulted in drug resistance. Reserving antibiotics and other antimicrobials is a promising way to combat these pathogens. Despite the antimicrobial surfaces that have been presented in the report having an intricate and prolonged synthetic process, their application is severely obstructed in practicality. For the new antibacterial nanocomposite, cerium metal-organic frameworks (Ce-MOF) that are ultrasmall in size, along with high stability, were developed as a vancomycin drug delivery system. The composite's pH-responsive synergistic antibacterial mechanism enables controlled release of vancomycin. According to the study, Ce-MOF@ Vanc can take advantage of its reversible Ce/Ce conversion to play a role in different physiological microenvironments, while exhibiting excellent biocompatibility. The Ce-MOF@ Vanc composite was strongly bonded to titanium alloy surfaces due to the adhesive properties of dopamine. This series of coatings exhibited highly effective antibacterial performance against S. aureus and E. coli, attributable to the synergistic action of Ce-MOF and vancomycin in membrane disruption and biochemical inhibition. The most effective coating was found to have an antibacterial efficiency of 96.62%. It showed excellent durability and corrosion resistance properties from wear and electrochemical tests. Also, the TCMV 3 coating had the highest corrosion inhibition efficiency, which was 93.22%. As a result of this research work, a new strategy was given, and an experimental basis was provided for the development of next-generation intelligent implant surfaces with the function of anti-bacteria, anti-corrosion, and biosafety.
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