Zhongguo Ying Yong Sheng Li Xue Za Zhi [JOURNAL]

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[An improved method about culture and identification of primary rat bone marrow derived mesenchymal stem cells].

Ma HG, Liu HQ, Tang YY

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308427 · Publisher ↗

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[Effects of bosutinib on the malignant behavior of thyroid papillary carcinoma B-CPAP cells and its mechanisms].

Xia HB, Wan WJ, Wang Y … +4 more , Zhang YF, Cao WZ, Li S, Wu C

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308426 · Publisher ↗

To investigate the effects of bosutinib on the malignant behavior of thyroid papillary carcinoma B-CPAP cells and its possible mechanisms. Thyroid papillary carcinoma B-CPAP cells were cultured in vitro with a concentra... To investigate the effects of bosutinib on the malignant behavior of thyroid papillary carcinoma B-CPAP cells and its possible mechanisms. Thyroid papillary carcinoma B-CPAP cells were cultured in vitro with a concentration gradient of(1、2、3、4 and 5 μmol/L)bosutinib intervened for 24 hours, DMSO was used as the control group. Five parallel compound holes were set in each group. Cell counting kit (CCK-8 method) method was used to detect cell proliferation. Transwell assay and cell wound healing assay were used to detect cell invasion and migration. TUNEL staining assay and flow cytometry were used to detect cell apoptosis. Western blot was used to detect the expressions of autophagic proteins (Beclin-1, LC3, p62) and signal pathway proteins (SIK2, p-mTOR, mTOR, p-ULK1, ULK1). Compared with the control group, the cell proliferation activity, migration ability and invasion ability were decreased (＜0.01), while the cell apoptosis rate was increased (＜0.01) in the bosutinib concentration groups of 2, 3, 4 and 5 μmol/L . In the concentration groups of 4 and 5 μmol/L, the expression of Beclin-1 (＜0.05), LC3- Ⅱ/LC3- Ⅰ (＜0.05), SIK2 (＜0.01) and p-ULK1 (＜0.01) protein was decreased, while the expression of p62 (＜ 0.05) and p-mTOR (＜0.01) protein was increased. Bosutinib may inhibit the autophagy of thyroid papillary carcinoma cells through SIK2-mTOR-ULK1 signaling pathway to inhibit their proliferation, invasion and migration and promote apoptosis, thereby weakening their malignant behavior.

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[Mechanisms of Sulforaphane in the treatment of brain damage caused by acute carbon monoxide poisoning based on network pharmacology].

Yue AC, Song HP, Zhou XD … +2 more , Han W, Li Q

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308425 · Publisher ↗

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[Effects of mitophagy on aerobic exercise intervention for depression in rats].

Lyu YY, Gao QJ, Zhang JM … +3 more , Li X, Jin Y, Wang L

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308424 · Publisher ↗

This experiment was designed to observe the effects of aerobic exercise on depressive behavior in rats induced by chronic unpredictable mild stress (CUMS), and to explore the possible mechanism by detecting the proteins... This experiment was designed to observe the effects of aerobic exercise on depressive behavior in rats induced by chronic unpredictable mild stress (CUMS), and to explore the possible mechanism by detecting the proteins related to mitochondrial autophagy. SD rats were randomly divided into three groups: blank control group (C, =12), depression model group (D, =12) and post-depression exercise group (D+E, =12). Group D and D+E were modeled with CUMS for 28 days, and group D+E underwent aerobic exercise intervention for 4 weeks after model establishment. Then the behavior of rats was evaluated. The concentrations of whole brain dopamine and norepinephrine were determined by ELISA kits. The morphology and structure of mitochondria in the frontal lobe were observed with the transmission electron microscope (TEM). Mitochondrial autophagy lysosomes were localized by immunofluorescence colocalization. The expressions of LC3 and P62 proteins in the frontal lobe were measured with Western Blotting. The relative content of mitochondrial DNA was detected using Real-time PCR. ①Compared with group C, the sucrose preference ratio in group D was decreased significantly(＜0.01); Compared with group D, the sucrose preference ratio in group D+E was increased significantly (＜0.01). ②In the open field experiment, compared with group C, group D had a significant decrease in activity, average speed and total distance (＜0.05); Compared with group D, the average rate of activity in group D+E was significantly higher (＜0.05). ③ELISA results showed that the levels of whole brain dopamine and norepinephrine were significantly lower in group D rats than those in group C (＜0.05). ④Under transmission electron microscopy, compared with group C, group D had different degrees of mitochondrial swelling, decreased crest density, and intermembrane space dilation.; Compared with group D, a significant increase in mitochondrial autophagosomes and autophagic lysosomes was observed in neurons in group D+E. Increased co-localization of mitochondria with lysosomes in the D+E group could be observed under fluorescence microscopy. ⑤Compared with group C, the expression of P62 was increased significantly(＜0.05), and LC3II/LC3I ratio was decreased significantly (＜0.05) in group D; Compared with group D, LC3II/LC3I ratio was significantly higher in group D+E than that in group D (＜0.05). ⑥Compared with group C, the relative number of mitochondrial DNA in the frontal lobe of group D was increased significantly (＜0.05). Aerobic exercise has a significant improvement effect on depression induced by CUMS in rats, and its mechanism may be related to the upregulation of the level of linear autophagy.

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[Effects of exhaustive exercise on coagulation state in rats].

Xia YS, Gao H, Zhao DW

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308423 · Publisher ↗

To investigate the effects of one-time exhaustive exercise on coagulation state in rats and its mechanism. Forty-eight SD rats were randomly divided into control group and exhaustive exercise group, 24 rats in each grou... To investigate the effects of one-time exhaustive exercise on coagulation state in rats and its mechanism. Forty-eight SD rats were randomly divided into control group and exhaustive exercise group, 24 rats in each group. Rats in exhaustive exercise group were trained with treadmill training for 25～50 min at a time on non-slope treadmill and the initial speed of 5 m/min was uniformly accelerated to 25 m/min until the rats exhausted. Thrombelastography (TEG) was used to monitor the coagulation function of rats after training. The ligation model of inferior vena cava (IVC) was established to evaluate thrombosis. The phosphatidylserine (PS) exposure and Ca concentration were detected by flow cytometry. The production of FXa and thrombin was detected by microplate reader. The clotting time was measured by using coagulometer. Compared with the control group, the blood of rats in the exhaustive exercise group exhibited hypercoagulable state. The probability of thrombus formation, weight, length and ratio in the exhaustive exercise group were significantly higher than those in the control group (＜0.01). The levels of PS exposure and intracellular Ca concentration of red blood cells (RBCs) and platelets in the exhaustive exercise group were increased significantly (＜0.01). The blood clotting time of RBCs and platelets was shortened (＜0.01), and the production of FXa and thrombin was increased significantly (＜0.01) in the exhausted exercise group, and both were inhibited by lactadherin (Lact, ＜0.01). The blood of exhaustive exercise rats is in a hypercoagulable state and the risk of thrombosis is increased. The increased PS exposure of RBCs and platelets caused by exhaustive exercise may be an important mechanism of thrombosis.

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[Effects of MICT/HIIT on the ultrastructure of myocardium and soleus in rats with high-fat diet and its mechanisms].

Wu WD, Wang Y, Wei JX

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308422 · Publisher ↗

To investigate the effects of moderate intensity continuous training (MICT) and high intensity intermittent training (HIIT) on the ultrastructure of myocardium and soleus in rats with high fat diet, and to explore the me... To investigate the effects of moderate intensity continuous training (MICT) and high intensity intermittent training (HIIT) on the ultrastructure of myocardium and soleus in rats with high fat diet, and to explore the mechanisms. 5-week-old male SD rats were randomly divided into normal diet quiet group (C), high-fat diet quiet group (F), high-fat MICT group (M) and high-fat HIIT group (H), with 8 rats in each group, and the fat content of the high-fat dietary feed was 45%. The M and H groups were given 12 weeks of treadmill running with an incline of 25°. The M group was given continuous exercise with 70%VOmax intensity, and the H group was given intermittent exercise with 5 min 40%~45%VOmax and 4 min 95%~99%VOmax intensity successively. After the intervention, the contents of free fatty acid (FFA), triglyceride (TG), high density lipoprotein cholesterol (HDL) and low density lipoprotein cholesterol (LDL) in serum were detected. Transmission electron microscopy was used to observe the ultrastructure of myocardium and soleus in rats. Western blot was used to detect the protein expressions of AMPK, malonyl-CoA decarboxylase (MCD) and carnitine palmitoyl transterase 1 (CPT-1) in myocardium and soleus. Compared with C group, the body weight, Lee's index, the contents of LDL, TG and FFA in serum were increased, the content of HDL was decreased (＜0.05), the protein expressions of AMPK and CPT-1 in myocardium and soleus were increased, the protein expression of MCD was decreased (＜0.05), and the ultrastructure was damaged in group F. Compared with F group, the body weight and Lee's index were decreased, the contents of LDL and FFA in serum were decreased (＜0.01), the protein expressions of AMPK, MCD and CPT-1 in myocardium were increased, and the protein expressions of AMPK and MCD in soleus were increased (＜0.05), and the ultrastructural damage was attenuated in M and H groups. Compared with M group, the content of HDL in serum was increased (＜0.01), the protein expressions of AMPK and MCD in myocardium were increased, and the ultrastructural damage was mild, the protein expression of AMPK in soleus was decreased, the protein expression of MCD in soleus was increased (＜0.05), and the ultrastructural damage was severe in group H. MICT and HIIT have different effects on the ultrastructure of myocardium and soleus in high-fat diet rats by intervening the protein expression of AMPK, MCD and CPT-1.

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[Relationship between exhaustion time and blood oxygen saturation of brain and muscle tissues at the moment of exhaustion under high temperature environment].

Wang DL, Lang J

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308421 · Publisher ↗

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[Synergistic effects of bicuculline combined with early running on the recovery of brain injury in rats].

Liu NH, Wang DQ, Peng ZF

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308420 · Publisher ↗

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[Effects of endurance exercise on glucose uptake of skeletal muscle in mice and regulation mechanism of interleukin 15].

Wang Z, Wang Q, Wu HD … +4 more , Hu YQ, Peng ZF, Guo XY, Jiang N

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308419 · Publisher ↗

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[Effects of whole body vibration on bone strength and physical fitness in elderly COPD patients complicated with osteoporosis].

Chen SW, Yi J

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308418 · Publisher ↗

To investigate the effects of adding whole body vibration (WBV) to routine exercise regimen of pulmonary rehabilitation (PR) on bone strength, lung function and exercise ability of elderly patients with stable chronic ob... To investigate the effects of adding whole body vibration (WBV) to routine exercise regimen of pulmonary rehabilitation (PR) on bone strength, lung function and exercise ability of elderly patients with stable chronic obstructive pulmonary disease (COPD) complicated with osteoporosis (OP). Thirty seven elderly patients with stable COPD were randomly divided into control group (group C, =12, age: 64.6±3.8 years), conventional PR group (PR group, =12, age: 66.1±4.9 years), and whole body vibration combined PR group (WP group, =13, age: 65.5±3.3 years). Before intervention, X-ray and computerized tomography bone scan, bone metabolic markers, pulmonary function, cardiopulmonary exercise, 6-minute walking and isokinetic muscle strength were performed, and then intervened for 36 weeks, three times/week, among which group C subjects were given routine treatment, PR group added aerobic running and static weight resistance on the basis of routine treatment, and WP group added WBV on the basis of PR group intervention. After the intervention, the same indicators were detected. Compared with before the intervention, the pulmonary function indexes of each group were significantly improved after the intervention (＜0.05), and the bone mineral density and bone microstructure indexes of the patients in the WP group were also significantly improved (＜0.05). Compared with group C and group PR, the bone mineral density, bone microstructure, parathyroid hormone (PTH), insulin-like growth factor-1 (IGF-1), interleukin-6 (IL-6), osteocalcin (OCN) and other bone metabolism indexes, knee flexion, peak extension torque, fatigue index and muscle strength of patients in WP group were significantly improved (＜0.05). Adding WBV to the conventional PR regimen can improve the bone strength, lung function and exercise capacity of elderly patients with COPD complicated with OP, and may be able to make up for the deficiency of the current conventional PR regimen for insufficient muscle and bone stimulation.

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[Effects of adipokine chemerin on the improvement of islet function in diabetic mice by aerobic exercise and its mechanisms].

Zhang QL, Qu J, Wang XH

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308417 · Publisher ↗

To investigate the effects of adipokines chemerin on the improvement of islet function caused by exercise in mice with diabetes, and the possible mechanism of glucagon-like peptide 1 (GLP-1). Male ICR mice were randomly... To investigate the effects of adipokines chemerin on the improvement of islet function caused by exercise in mice with diabetes, and the possible mechanism of glucagon-like peptide 1 (GLP-1). Male ICR mice were randomly divided into a control group fed with normal diet (Con, =6) and a diabetic modeling group fed with 60% kcal high-fat diet (=44). After 6 weeks, the diabetic modeling group was once given a fasting intraperitoneal injection of streptozotocin (100 mg/kg). The successfully modeled mice were divided into diabetes group (DM), diabetes plus exercise group (EDM), and diabetes plus exercise and exogenous chemerin group (EDMC), 6 in each group. Mice in exercise groups participated in a six-week modest intensity treadmill running exercise with a gradually increased load. Mice in the EDMC group were intraperitoneally injected with exogenous chemerin (8 μg/kg) from the 4th week of the exercise period, six days per week, and one time per day. And the other groups were untreated. Adipose chemerin knockout mice were constructed. Then they and the control mice were divided into 6 groups (=4): Normal diet control group (Con-ND), Normal diet chemerin knockout heterozygote mice group (Chemerin(+/-)-ND), Normal diet chemerin knockout homozygotes mice group(Chemerin(-/-)-ND), High-fat diet control group (Con-HFD), High-fat diet chemerin knockout heterozygote mice group (Chemerin(+/-)-HFD), High-fat diet chemerin knockout homozygotes mice group (Chemerin(-/-)-HFD). They were fed with normal or high-fat diet for 11 weeks and oral glucose tolerance test (OGTT) was conducted. After the mice of each group were executed under anesthesia, the samples such as pancreas and colon were collected. Fasting blood glucose (FBG) and fasting insulin (FINS) levels in mice were measured, and the insulin resistance index (HOMA-IR) was calculated. HE staining was used to observe the structure of islets. ELISA was used to detect the GLP-1 level in serum. The mRNA levels of proglucagon (GCG) and chemerin in the colon were measured by real-time PCR. And the protein levels of GCG and chemerin in the colon were detected by Western blot. Compared with the DM group, the vacuolar degeneration and shrinkage of islet cells in the EDM group were reduced, the islet structure was improved, while the levels of FINS, HOMA-IR and FBG were decreased significantly (＜0.05 or ＜0.01). The colon and serum chemerin levels were decreased significantly(＜0.05), while the colonic GCG mRNA and protein levels were increased significantly (＜0.05 or ＜0.01). Compared with the EDM group, the islet cells in the EDMC group were shrunken, with unclear borders. The structure of the islets was damaged, and the levels of FINS, HOMA-IR and FBG were increased significantly (＜0.01), while the mRNA and protein levels of GCG were decreased significantly (＜0.05 or ＜0.01). Compared with the Con-HFD group, the blood glucose at 30, 90 and 120 min after oral glucose in the chemerin (-/-)-HFD group was significantly lower (＜0.01), and the area under the blood glucose time curve was significantly lower (＜0.01). The islets had clear structure, regular shape and well-defined boundaries, while the serum GLP-1 and colonic GCG protein levels were increased significantly(＜0.05). Aerobic exercise improves the structure and function of pancreatic islets by reducing the level of chemerin in diabetes mice, which is related to the negative regulation of chemerin on GLP-1 level.

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[KLF15/mTOR related proteins involved in effect of aerobic interval training on improving skeletal muscle lesions in rats with type 2 diabetes].

Liao ZX, Huang L, Zhu HZ … +1 more , Zhu MJ

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308416 · Publisher ↗

To investigate the effects of aerobic intermittent exercise on the expressions of KLF15/mTOR related proteins to improve skeletal muscle lesions in type 2 diabetes rats. The experimental model of type 2 diabetes rats wa... To investigate the effects of aerobic intermittent exercise on the expressions of KLF15/mTOR related proteins to improve skeletal muscle lesions in type 2 diabetes rats. The experimental model of type 2 diabetes rats was established by feeding high-fat diet for 4 weeks and intraperitoneal injection of streptozotocin (STZ). After modeling, rats were randomly divided into two groups: diabetes model group (DM), diabetes+exercise group (DE), and normal rats were set as control group (C), 10 rats in each group. Group DE was given 8-week aerobic intermittent treadmill exercise intervention, while group C was not given any intervention. At the end of the experiment, the expressions of KLF15, mTOR, p-mTOR, and cleared caspase-3 in gastrocnemius muscle were detected by Western blot. The histopathologic changes of gastrocnemius were observed under microscope; skeletal muscle cells apoptosis rates and muscle mass were examined respectively using HE staining and TUNEL fluorescence staining. At the same time, changes of blood glucose and serum insulin, and weight were examined in the end of the experiment. ①Compared with group C, the wet weight of gastrocnemius muscle and body weight, ratio of wet gastrocnemius muscle and body weight in group DM were decreased(＜0.05 or ＜0.01); compared with group DM, the wet weight of gastrocnemius muscle, ratio of wet gastrocnemius muscle and body weight in the group DE were increased significantly (＜0.05). ②Compared with group C, the fasting blood glucose level of group DM was increased significantly (＜0.01), while serum insulin level of the group DM was decreased significantly(＜0.01);compared with group DM, the above indexes were opposite in the group DE with intervention(＜0.05). ③Compared with group C, the morphology of skeletal muscle cells in group DM was abnormal, the number of muscle nuclei was increased, the transverse lines were blurred and disappeared, the sarcomere was broken, and some muscle fibers were dissolved. Compared with group DM, the abnormal cell morphology, segmental injury of sarcomere and dissolution of muscle fibers in group DE were improved. The sarcolemma was more complete and the arrangement of muscle nuclei was more orderly. ④Compared with group C, the expressions of KLF15 and cleaved caspase-3, cells apoptosis rates in group DM were increased significantly(＜0.01), while p-mTOR/mTOR level was decreased(＜0.01) ; compared with group DM, the above indexes were opposite in the group with intervention(＜0.05 or ＜0.01). Aerobic intermittent exercise is beneficial to improve the skeletal muscle pathological changes in type 2 diabetes rats, which may be due to the effective regulation of KLF15/mTOR related protein expression and the reduction of apoptosis damage.

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[Effects of on insulin resistance in obese rats and its mechanisms].

Zhang SJ, Zhang J, Guo JP … +1 more , Niu YP

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308415 · Publisher ↗

To investigate the effects of Rosa roxburghii on insulin resistance in obese rats and the regulation of phosphatidylinositol 3-kinase (PI3K)/ protein kinase Bβ(PKB/Akt2)/ glucose transporter 4(GLUT4) signaling pathway.... To investigate the effects of Rosa roxburghii on insulin resistance in obese rats and the regulation of phosphatidylinositol 3-kinase (PI3K)/ protein kinase Bβ(PKB/Akt2)/ glucose transporter 4(GLUT4) signaling pathway. Five-week-old male SD rats were randomly divided into normal control group (NC), model group (M), positive control group (PC), low-dose rosa roxburghii group (LD) and high-dose rosa roxburghii group (HD), with 10 rats in each group. The rats in the NC group were fed with normal diet, while those in the M, PC, LD and HD groups were fed with high-fat diet. From the 13th week, according to the dose standard of 6 ml/kg, rats in the LD group were intragastrically administered with 100 mg/kg Rosa roxburghii Tratt, the HD group were treated with 300 mg/kg Rosa roxburghii Tratt, the PC group were treated with 0.11 g/kg Chiglitazar sodium, and the NC and M groups were intragastrically administered with the same volume of normal saline. The body weight was measured every week until 20 weeks. The rats were sacrificed 24 h after the last experiment. Blood and skeletal muscle were collected. Serum total cholesterol (TC) and triglyceride (TG) contents were detected by colorimetric method, serum superoxide dismutase (SOD) activity was detected by xanthine oxidase method, serum malondialdehyde (MDA) content was detected by thiobarbituric acid method, blood glucose (FBG) value was detected by glucose oxidase method, insulin (FINS) content was detected by ELISA, and PI3K, Akt2, and GLUT4 protein and gene expressions were detected by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). Compared with the NC group, the body weight, serum MDA, TG, TC, FBG, FINS, HOMA-IR levels in the M group were significantly increased (＜0.01), while SOD activity, PI3K、Akt2、GLUT4 protein and mRNA expression levels were significantly increased(＜ 0.01). Compared with group M, the body weight, serum MDA, TG, TC, FBG, FINS, and HOMA-IR were decreased significantly in LD group, HD group and PC group (＜0.05 or ＜0.01), while SOD activity, PI3K, Akt2, GLUT4 protein and mRNA expression levels were increased significantly (＜0.05 or ＜0.01). can improve insulin resistance in obese rats by antioxidant stress and up-regulating the expressions of PI3K, Akt2, and GLUT4 proteins and genes, which may be related to the PI3K/Akt2/GLUT4 signaling pathway.

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[Protective effects of salidroside on vascular endothelial cells in rats with frostbite after chronic hypoxia].

Wang HJ, Li Y, Feng YP … +2 more , Zhang KW, Peng MD

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308414 · Publisher ↗

To investigate the protective effects of salidroside on endothelial cells in rats with frostbite after chronic hypoxia. Healthy male SD rats, randomly divided into 3 groups with 10 rats in each group, which included the... To investigate the protective effects of salidroside on endothelial cells in rats with frostbite after chronic hypoxia. Healthy male SD rats, randomly divided into 3 groups with 10 rats in each group, which included the sham injury group, the model group, and the model +salidroside group. The rats in each group were placed in a composite low-pressure chamber to simulate a environment with a pressure of 54.1 kpa and a temperature of 23~25°C. The rats were exposed to hypoxia under these conditions for 14 days, during the experimental time the rats in the model+salidroside group were treated with 50 mg/kg salidroside daily. After the rats were removed from the low-pressure chamber, except for the sham injury group, frozen iron sheets were applied tightly to the back of the rats for 30 s, supplemented with low temperature for frostbite modeling. Blood and skin tissues were collected at 12 hours after modeling for testing. The structural changes in tissue and vascular endothelial cells were observed in the frostbite region. Vascular endothelial cell particulate EMP levels were detected. The levels of ICAM-1, sEPCR, vWF, ET-1 and NO secretion were determined. The expression levels of HIF-1α, p-PI3K, p-Akt and VEGF were detected by Western blot. Salidroside could effectively reduce skin collapse in frostbitten areas. It could reduce the injury of frostbitten tissues, and improve the subcutaneous tissue necrosis and inflammatory cell infiltration. The autophagy of vascular endothelial cells was reduced. Compared with the model group (0.250±0.165)%, the expression of EMPs in the model+salidroside group (2.453±0.196)% was increased significantly (＜0.01). In addition, the contents of NO (2.622±0.219)pg/ml was also significantly higher than that of the model group (1.616±0.152)pg/ml (＜0.01), and the content of vWF (233.50±13.43)pg/ml was lower than that of the model group (315.60±8.78)pg/ml (＜0.05). There was no significant difference in the levels of ICAM-1, sEPCR and ET-1. Salidroside significantly decreased the expressions of p-PI3K, p-Akt, VEGF and HIF-1α protein in vascular endothelial cells of rats with frostbite (＜0.01). Salidroside can reduce endothelial cell damage, reduce endothelial cell autophagy and promote endothelial cell regeneration. Based on the PI3K/Akt pathway, salidroside has a good protective effect on endothelial cells of rats with frostbite after chronic hypoxia.

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[The antifatigue effects of Ginseng compound on mice in acute hypoxic environment].

Zhao L, Li M, Ma BH … +3 more , Shi WH, Wang R, Yin DF

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308413 · Publisher ↗

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[Effects of PBMC mitochondrial autophagy on exercise energy consumption and mitochondrial respiratory chain complex enzyme activity in high altitude migrants].

Kong HJ, Wang FH, Li XL … +2 more , Gan SQ, Chen X

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308412 · Publisher ↗

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[Effects of panax notoginseng saponins on pulmonary vascular remodeling in rats with pulmonary hypertension and its mechanisms].

Song ZY, Wang XY, Tian YN … +4 more , Li ZL, Wang XT, Yuan LB, Wang WT

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308411 · Publisher ↗

To investigate the effects of panax notoginseng saponins (PNS) on pulmonary vascular remodeling and SIRT1/FOXO3a/p27 pathway in pulmonary arterial hypertension (PAH) rats. Male SD rats weighing 200~250g were randomly di... To investigate the effects of panax notoginseng saponins (PNS) on pulmonary vascular remodeling and SIRT1/FOXO3a/p27 pathway in pulmonary arterial hypertension (PAH) rats. Male SD rats weighing 200~250g were randomly divided into control group, monocrotaline group (MCT) and monocrotaline + panax notoginseng saponins group (MCT+PNS), with 10 rats in each group. The rats in control group were injected intraperitoneally with normal saline 3 ml/kg on the first day, then injected intraperitoneally with normal saline 2.5 ml/kg every day. The rats in MCT group were injected intraperitoneally with MCT 60 mg/kg on the first day, followed by daily injection of normal saline 2.5 ml/kg. In MCT+PNS group, 60 mg/kg MCT was injected intraperitoneally on the first day, and 50 mg/kg PNS was injected intraperitoneally every day. The above models were fed conventionally for 4 weeks. After the modeling was completed, the mean pulmonary artery pressure (mPAP) and right ventricular systolic pressure (RVSP) of rats in each group were detected by right heart catheter method, weighed and calculated right ventricular hypertrophy index (RVHI), and the pulmonary vascular structure and morphological changes were observed by HE and Masson staining. The protein and gene expressions of SIRT1, FOXO3a, p27, PCNA and Caspase-3 were detected by qPCR and Western blot. Compared with control group, mPAP, RVSP and RVHI in MCT group were increased significantly (＜0.01), pulmonary vessels were thickened significantly and collagen fibers were increased, protein and gene expressions of SIRT1, FOXO3a, p27 and Caspase-3 were decreased (＜0.05 or ＜0.01). The protein and gene expressions of PCNA were increased (＜0.05). Compared with MCT group, the levels of mPAP, RVSP and RVHI in MCT+PNS group were decreased significantly (＜0.05 or ＜0.01), pulmonary vascular thickening was alleviated and collagen fibers were reduced. The protein and gene expressions of SIRT1, FOXO3a, p27 and Caspase-3 were increased (＜0.05 or ＜0.01), while the protein and gene expressions of PCNA were decreased (＜0.05 or ＜0.01). Panax notoginseng saponins can relieve pulmonary vascular remodeling in rats with pulmonary hypertension by activating SIRT1/FOXO3a/p27 pathway.

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[Resveratrol attenuates cardiac function impairment in plateau hypobaric hypoxia rats].

Xu HB, Song XN, Yan CQ … +6 more , Wang GR, Pu LL, Wang ZR, Wang XX, Chen ZL, Liu WL

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308410 · Publisher ↗

To study the protective effects of resveratrol (RSV) on cardiac function in rats with high altitude hypobaric hypoxia and its mechanisms. Thirty-six rats were randomly divided into control group, hypobaric hypoxia group... To study the protective effects of resveratrol (RSV) on cardiac function in rats with high altitude hypobaric hypoxia and its mechanisms. Thirty-six rats were randomly divided into control group, hypobaric hypoxia group (HH) and hypobaric hypoxia + RSV group (HH+RSV) according to the random number, 12 rats in each group. Rats in the HH and HH+RSV groups were subjected to chronic long-term high altitude hypobaric hypoxia intervention for 8 weeks in a hypobaric chamber at a simulated altitude of 6 000 m for 20 h / d. The rats of HH + RSV were fed with RSV at a dose of 400 mg/(kg·d). The rats were tested once a week for body weight and twice a week for food intake. Before execution, the rats were tested by blood cell analyzer for routine blood parameters and echocardiogram for cardiac function parameters in each group. The routine blood indexes of each group were measured by blood cell analyzer, the cardiac function indexes of each group were measured by echocardiography, myocardial hypertrophy was evaluated by HE staining, myocardial tissue reactive oxygen levels were evaluated by dihydroethidium (DHE) staining. Oxidative stress was evaluated by serum and myocardial tissue total antioxidant capacity (T-AOC), superoxide dismutase activity (SOD) and malondialdehyde (MDA) content. Compared with the C group, the body mass and food intake of rats were decreased significantly (＜0.05) in HH group, while compared with the C group, RSV had no significant effects on the body mass and food intake of rats in the HH+RSV group (＞0.05). Compared with the C group, the levels of erythrocytes and hemoglobin of rats in the HH group were increased significantly (＜0.05), while the platelet concentration was decreased significantly(＜0.05); compared with the HH group, the erythrocyte and hemoglobin levels were decreased significantly (＜0.05) and platelet concentration was increased significantly(＜0.05) in rats of the HH+RSV group. Compared with the C group, the cardiac coefficient, myocardial fiber diameter and thickness were significantly increased in the HH group (＜0.05); compared with the HH group, the cardiac coefficient and myocardial fiber thickness were significantly decreased in the HH+RSV group (＜0.05). Echocardiographic analysis showed a significant increase in ventricular wall thickness (＜0.05) and a significant decrease in ejection fraction and cardiac output (＜0.05) in the HH group compared with the C group, and a significant decrease in ventricular wall thickness and a significant improvement in cardiac function (＜0.05) in the HH+RSV group compared with the HH group. The results of DHE staining showed that myocardial tissue reactive oxygen levels were increased significantly in the HH group compared with the C group (＜0.05); myocardial tissue reactive oxygen levels were significantly restored in the HH+RSV group compared with the HH group (＜0.05). The oxidative/antioxidant results showed that the serum and myocardial T-AOC and SOD activities were decreased significantly (＜0.05) and the MDA level was increased significantly (＜0.05) in the HH group compared with the C group; the serum and myocardial T-AOC and SOD activities were increased significantly (＜0.05) and the MDA level was decreased significantly(＜0.05) in the HH+RSV group compared with the HH group. Long-term plateau hypobaric hypoxia exposure leads to myocardial hypertrophy and reduced cardiac function in rats. Resveratrol intervention significantly improves myocardial hypertrophy and cardiac function in rats caused by altitude hypobaric hypoxia exposure, which is closely related to reducing of reactive oxygen species and improving myocardial oxidative stress levels.

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[Intervention effects of estradiol on myocardial ischemia- reperfusion injury of rat and its mechanisms].

Feng JR, Zhang HY, Shi H … +4 more , Wang TF, Wang ZJ, Cheng GH, Bi SL

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308409 · Publisher ↗

To study the effects of estradiol (E2) on alleviating myocardial ischemia/reperfusion(I/R) injury through estrogen receptorβ(ERβ) mediated extracellular regulated protein kinases(ERK) pathway activation. Eighty-four adu... To study the effects of estradiol (E2) on alleviating myocardial ischemia/reperfusion(I/R) injury through estrogen receptorβ(ERβ) mediated extracellular regulated protein kinases(ERK) pathway activation. Eighty-four adult female SD rats were ovariectomized and randomly divided into control group, NC siRNA adeno-associated virus (AAV) group received sham operation, the myocardial I/R injury model was prepared by ligation of the left anterior descending coronary artery in I/R group, E2+I/R group, NC siRNA AAV+I/R group, NC siRNA AAV+E2+I/R group and ERβ-siRNA AAV+E2+I/R group. E2+I/R group, NC siRNA AAV+E2+I/R group and ERβ-siRNA AAV+E2+I/R group were treated with E2 0.8 mg/kg by gavage for 60 days before modeling. NC siRNA AAV+I/R group, NC siRNA AAV+E2+I/R group, and ERβ-siRNA AAV+E2+I/R group were treated with AAV by caudal vein injection 24 h before modeling. After 120 min of reperfusion, the contents of serum lactate dehydrogenase (LDH), phosphocreatine kinase (CK), phosphocreatine kinase isoenzyme (CK-MB), myocardial infarction area and the expressions of ERβ, p-ERK, the contents of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1 β), malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in myocardium were measured. The contents of serum LDH, CK, CK-MB, myocardial infarction area and the contents of TNF-α, IL-1 β, MDA in myocardium of I/R group were higher than those of the control group, the expression levels of ERβ and p-ERK and the content of T-AOC were lower than those in the control group (＜0.05). The contents of serum LDH, CK and CK-MB, myocardial infarction area and the contents of TNF-α, IL-1 β and MDA in myocardium of E2+I/R group were lower than those of the I/R group, the expression levels of ERβ and p-ERK and the content of T-AOC were higher than those of the I/R group(＜0.05). After knockdown ERβ by caudal vein injection of ERβ-siRNA AAV, the contents of serum LDH, CK and CK-MB, myocardial infarction area and the contents of TNF-α, IL-1 β and MDA in myocardium of ERβ-siRNA AAV+E2+I/R group were higher than those of NC-siRNA AAV+E2+I/R, the expression levels of ERβ and p-ERK and the content of T-AOC were lower than those of NC-siRNA AAV+E2+I/R(＜0.05). E2 has protective effects on myocardial I / R injury in ovariectomized rats, which are related to the promotion of ERβ mediating the activation of ERK pathway, reducing inflammatory and oxidative stress responses.

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[Effect of epithelial-mesenchymal transition on cardiac fibrosis induced by oil mist particulate matter].

Liu X, Nie HP, Liu HL … +4 more , Shi Y, Lai WQ, Xi ZG, Lin BC

Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308408 · Publisher ↗

To investigate the effects of oil-mist particulate matter (OMPM) on cardiac tissue structure fibrosis in rats and the role of epithelial-mesenchymal transition (EMT). Six-week-old Wistar rats (half male and half female)... To investigate the effects of oil-mist particulate matter (OMPM) on cardiac tissue structure fibrosis in rats and the role of epithelial-mesenchymal transition (EMT). Six-week-old Wistar rats (half male and half female) were randomly divided into 3 groups: control group (without OMPM exposure), low-dose exposure group (50 mg/m) and high-dose exposure group (100 mg/m), 18 rats in each group, with 6.5 hours per day of dynamic inhalation exposure. After 42 days of continuous exposure, cardiac tissues were collected for morphological observation; Western blot was used to detect fibrosis markers collagen I and collagen III levels, epithelial marker E-cadherin levels, interstitial markers N-cadherin, fibronectin, vimentin, alpha-smooth muscle actin (α-SMA) levels, and EMT transcription factor Twist protein levels; Real-time polymerase chain reaction (RT-qPCR) was used to detect collagen I and collagen III mRNA levels. After OMPM exposure, myocardial cell edema and collagen fiber deposition were increased gradually with increasing exposure dose. Western blot results showed that compared with the control group, the expression levels of collagen I, collagen III, N-Cadherin, fibronectin, vimentin, α-SMA, and Twist protein were increased significantly in the low-dose exposure group and the high-dose exposure group (＜0.01), and protein expression levels were higher in the high-dose exposure group than those in the low-dose exposure group (＜0.01). In contrast, E-Cadherin protein expression levels were decreased significantly, and lower in the high-dose exposure group (＜0.01). RT-qPCR results showed that compared with the control group, collagen I and collagen III mRNA levels were increased significantly in the low-dose exposure group and the high-dose exposure group (＜0.01), and were increased with increasing exposure dose. (＜0.01). OMPM may induce cardiac fibrosis in rats by promoting EMT process.

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Zhongguo Ying Yong Sheng Li Xue Za Zhi [JOURNAL]

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