Braz Oral Res [JOURNAL]
Sun
200 papers
Oral cancer immunology: state of the art and future perspectives.
Girardi FM, Schuch LF, Martins MD
Braz Oral Res · 2024 · PMID 39775418 · Publisher ↗
Oral cancer is a multifactorial disease involving genetic, epigenetic, and environmental factors. The literature indicates that inflammatory cells at the advancing front of the tumor induce a host immune response, preven...
Oral cancer is a multifactorial disease involving genetic, epigenetic, and environmental factors. The literature indicates that inflammatory cells at the advancing front of the tumor induce a host immune response, preventing the spread of the tumor. However, cancer cells adopt various continued strategies to circumvent this immune surveillance. The complexity of immune mechanisms suggests that there must be virtually individual patterns of anti-tumor immune responses. Due to this important interaction of cancer with the immune system, the objective of the present study was to provide an up-to-date overview of immuno-oncology focused on oral cancer, summarizing the basic immunology, the classic risk factors, immunotherapy, and future treatment and prognostic perspectives.
Roughness profile and surface roughness after toothbrushing and degree of conversion of bulk-fill resins.
Terada RSS, Fronza BM, Faraoni JJ … +5 more , Hoshino LVC, Sato F, Palma-Dibb RG, Scheffel DLS, Giannini M
Braz Oral Res · 2024 · PMID 39775417 · Publisher ↗
The aim of this study was to assess roughness profile and surface roughness after simulated toothbrushing cycles, as well as the degree of conversion (DC) of bulk-fill resin composites at different depths. Forty nine com...
The aim of this study was to assess roughness profile and surface roughness after simulated toothbrushing cycles, as well as the degree of conversion (DC) of bulk-fill resin composites at different depths. Forty nine composite discs were made from three low-viscosity bulk-fill resins (Filtek Bulk-Fill Flowable/3M Oral Care - FBF, Beautifil-Bulk Flowable/Shofu Inc. - BBF and Surefill SDR Flow/Dentsply Caulk - SDR), three high-viscosity bulk-fill resin composites (Filtek Bulk-Fill Restorative/3M Oral Care - FBR, Beautifil-Bulk Restorative/Shofu Inc. - BBR and Tetric EvoCeram Bulk Fill/Ivoclar Vivadent - TEB) and one conventional composite (Filtek Supreme Ultra/3M Oral Care - FSU) (n=7), using addition silicon molds (2 mm thick x 10 mm diameter). The roughness profile (Rv) and surface roughness (Sa) were analyzed after 30,000 brushing cycles using confocal laser scanning microscopy. Five samples (4 mm thick x 2 mm diameter) were produced per resin composite and light-cured for 20 seconds using a LED-curing unit. After 24 hours at 37oC, DC of samples was evaluated at four depths, using a confocal Raman microscope. The data were analyzed by ANOVA and Tukey post hoc multiple-comparison tests (α = 0.05). The Rv values of FSU were lower than those obtained for BBR and BBF. The toothbrushing significantly decreased the Sa for all composites. The DC was significantly reduced at 4 mm depth. Toothbrushing changed composite surface of all materials, especially for BBR and BBF. Composites were not able to maintain the DC values shown at the top compared with those at the bottom (4 mm depth).
Crystallization by microwave energy: Effects on the survival probability of lithia-based glass ceramics.
Diamantino PS, Rodrigues CDS, Carvalho ABG … +7 more , Tribst JPM, Riquieri H, Ramos NC, Viegas DMDCCP, Bergamo ETP, Bonfante EA, Saavedra GSFA
Braz Oral Res · 2024 · PMID 39775416 · Publisher ↗
This study evaluated the survival probabilities of two lithia-based glass-ceramics after final crystallization in a microwave furnace using conventional crystallization as a reference. Disc-shaped samples of a lithium si...
This study evaluated the survival probabilities of two lithia-based glass-ceramics after final crystallization in a microwave furnace using conventional crystallization as a reference. Disc-shaped samples of a lithium silicate (LS, Celtra Duo) and a lithium disilicate (LD, e.max CAD) were prepared and divided into two groups according to the crystallization method (n = 30): microwave (M) or conventional furnaces (C). The biaxial flexural strength test was used to determine the fatigue test profile and its parameters. Then, specimens were submitted to an accelerated life test (step stress) using three profile levels - mild, moderate, and aggressive - varying the load increments and the number of cycles until fracture (4 Hz). Survival data were used to calculate Weibull's beta (β) value and reliability of the assigned missions. Scanning electron microscopy was employed to analyze surface morphology, fracture characteristics, and failure patterns. Beta (β) values for the LS-C, LS-M, LD-C, and LD-M groups were 2.65, 0.25, 0.62, and 0.3, respectively. Similar reliability was observed in all groups after 50,000 cycles at 100 and 150 Mpa. At 200 Mpa, the crystallization method did not affect the reliability within LS or LD. However, LD showed greater reliability than LS when crystallized by microwave energy. Thus, microwave energy can be suggested as an alternative to the process of conventional lithia-based glass-ceramics crystallization without damaging their survival probabilities.
Antimicrobial efficacy of natural-based endodontic solutions: a systematic review with a network meta-analysis.
Ferraz DC, Melo AP, Matos FS … +5 more , Paranhos LR, Moura CCG, Blumenberg C, Guerreiro-Tanomaru JM, Tanomaru-Filho M
Braz Oral Res · 2024 · PMID 39775415 · Publisher ↗
This systematic review aims to provide preclinical evidence of the antimicrobial efficacy of natural endodontic solutions (NES) compared to sodium hypochlorite (NaOCl) and chlorhexidine (CHX) against Enterococcus faecali...
This systematic review aims to provide preclinical evidence of the antimicrobial efficacy of natural endodontic solutions (NES) compared to sodium hypochlorite (NaOCl) and chlorhexidine (CHX) against Enterococcus faecalis. The study followed the PRISMA guidelines and had a registered protocol (PROSPERO - CRD42021224022). The inclusion criteria comprised ex vivo studies simulating root canal irrigation to assess the standardized mean difference of colony-forming units (CFUs). Records were systematically identified in PubMed, Scopus, EMBASE, SciELO, Web of Science, LILACS, DANS Easy, and BDTD databases in January 2024. The RoBDEMAT tool helped determine the risk of bias. As for the network meta-analysis, CFU data were collected pre- and post-irrigation protocols as an effect measure. Thirty-five studies met the eligibility criteria and were included in the qualitative analysis, and seven studies were eligible for the network meta-analysis. The total sample consisted of 1,504 root canals. Among the tested solutions, apple cider vinegar combined with 2.5% NaOCl showed the highest probability of reducing CFUs after root canal irrigation (95%CI: -0.76-0.20), followed by 2% CHX and NaOCl (considering the different concentrations [95%CI: -0.32-0.17]). The bias assessment revealed significant omissions in reporting, particularly regarding sample size calculation, sample randomization, and operator blinding. The obtained evidence demonstrated that, even though NES did not overcome NaOCl, they have antimicrobial potential against Enterococcus faecalis.
Socioeconomic inequalities in early childhood caries: evidence from vulnerable populations in Colombia.
Martignon S, Guarnizo-Herreño CC, Franco-Cortés AM … +6 more , García-Zapata LM, Ochoa-Acosta EM, Restrepo-Pérez LF, Arango MC, Cerezo MDP, Cortes A
Braz Oral Res · 2024 · PMID 39661799 · Full text
The Colombian Chapter of the Alliance-for-a-Cavity-Free-Future (Col-ACFF) has been conducting a health promotion and caries prevention program among young children in four vulnerable Colombian municipalities (baseline da...
The Colombian Chapter of the Alliance-for-a-Cavity-Free-Future (Col-ACFF) has been conducting a health promotion and caries prevention program among young children in four vulnerable Colombian municipalities (baseline data from 2012-2014). This study aimed to quantify socioeconomic inequalities in early childhood caries (ECC) and examine the potential role of daily fluoride-toothpaste use, previous-year dental-care visit, and nutrition/diet-related aspects. The study sample included 1344 children aged 1-5 years. Inequalities in the age-standardized prevalence rates of and mean number of tooth surfaces affected by moderate/extensive (dME) and initial (dIME) caries (defined using the ICDAS-merged-epi criteria) by household income and level of education were examined using the relative index of inequality (RII) and the slope index of inequality (SII). Approximately one-third of the children included in this study exhibited dME, while 84% exhibited dIME. The majority of outcomes exhibited social gradients, and significant relative (RII) and absolute (SII) inequalities in ECC were observed. The SII estimate indicated an absolute difference of 12.4% in the prevalence of moderate/extensive carious lesions among children living in households with the lowest compared to the highest education levels [SII: 12.4; 95% confidence interval (CI): 2.7-22.1]. These children were also 6.7 times more likely to exhibit dIME compared to those living in households with higher levels of education (SII:6.73 95% CI: 4.18-9.29). Daily use of fluoride toothpastes, dental care visits in the previous year, and nutrition/diet-related factors played a limited role in ECC inequalities. In conclusion, significant ECC inequalities were observed in these vulnerable populations, highlighting the importance of upstream and downstream interventions that raise awareness among stakeholders and improve community- and individual-based practices to address this.
Scientific contribution of the Brazilian CNPq Research Productivity fellows in dentistry.
Oliveira VGB, Sousa ÁAD, Oliveira FES … +4 more , Martelli DRB, Araújo E, Coletta RD, Martelli Júnior H
Braz Oral Res · 2024 · PMID 39661798 · Full text
This study evaluated the scientific contribution of Brazilian CNPq Research Productivity fellows (PQ) in Dentistry by areas of activity. This cross-sectional study included 217 active PQ who were grouped into six groups:...
This study evaluated the scientific contribution of Brazilian CNPq Research Productivity fellows (PQ) in Dentistry by areas of activity. This cross-sectional study included 217 active PQ who were grouped into six groups: (1) Biomaterials, Prosthodontics, and Restorative Dentistry; (2) Public Health and Epidemiology; (3) Oral Pathology, Stomatology, and Dental Radiology; (4) Pediatric Dentistry and Child Health; (5) Dental Clinic (Periodontics, Endodontics, Orthodontics, Oral Surgery, and Implantology), and (6) Basic Areas (Histology, Biochemistry, Molecular Biology, Physiology, Microbiology, Immunology, and Pharmacology). The PQ were predominantly male (n = 122; 56.2%), received level 2 scholarships (n = 121; 55.8%), and performed research in the Southeast region of Brazil (n = 160; 73.73%). Regarding supervision of undergraduate, master's, and PhD students, both during their entire careers and in the last 5 years, the highest average was observed for PQs in the field of Public Health and Epidemiology, the only area with higher average supervision of master's than that of undergraduate and PhD students. PQ in Public Health and Epidemiology had the highest average number of papers published over their career and in the last 5 years, followed by PQ in Pediatric Dentistry and Child Health and Dental Clinic. The high productivity of PQ is demonstrated by modern research performance indicators. Their scientific publications are indexed in bibliometric databases such as WoS, Scopus, and SciELO. Addtionally, highlighted among the PQ was the time since initiation of their scientific careers and master's and doctoral candidates trained.
In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate.
Ferrari CR, Kitamoto KSA, Pelá VT … +6 more , Taira ÉA, Araújo TT, Thomassian LTG, Henrique-Silva F, Pessan JP, Buzalaf MAR
Braz Oral Res · 2024 · PMID 39661797 · Full text
The objective of this in vitro study was to assess the efficacy of CaneCPI-5, either alone or in combination with various concentrations of sodium trimetaphosphate (TMP) in protecting against initial enamel erosion. A to...
The objective of this in vitro study was to assess the efficacy of CaneCPI-5, either alone or in combination with various concentrations of sodium trimetaphosphate (TMP) in protecting against initial enamel erosion. A total of 135 bovine enamel specimens were prepared and categorized into nine groups (n/group=15) according to the following treatments: Deionized water; Commercial solution (Elmex Erosion ProtectionTM); 0.1 mg/mL CaneCPI-5; 0.5% TMP; 1.0% TMP; 3.0% TMP; 0.1 mg/mL CaneCPI-5+0.5% TMP; 0.1 mg/mL CaneCPI-5+1.0%TMP; and 0.1 mg/mL CaneCPI-5+3.0%TMP. The specimens were treated with the respective solutions for 2 h, followed by acquired enamel pellicle formation for 2 h and exposure to 0.65% citric acid (CA) for 1 min. These procedures were repeated once a day for three consecutive days. Demineralization was assessed by the percentage change in surface hardness (%CSH) and calcium release into CA, analyzed by the Arsenazo III method. The data were evaluated using Kruskal-Wallis/Dunn's tests. Regarding %CSH, CaneCPI-5+3.0%TMP was the most effective treatment when compared to the CaneCPI-5 group alone. As for calcium release into CA, the CaneCPI-5+0.5% TMP and CaneCPI-5 groups (both with lower calcium release) did not significantly differ from the commercial solution. In conclusion, combination of CaneCPI-5 with TMP enhances the protective potential against initial enamel erosion in vitro.
Effect of curcumin and three analogues on pre-osteoblast cells' viability, differentiation, and gene expression.
Sá AF, Diniz IMA, Oliveira RB … +5 more , Diniz MG, Cortés ME, Souza LL, Olórtegui CDC, Lages FS
Braz Oral Res · 2024 · PMID 39661796 · Full text
Curcumin, found in turmeric rhizomes (Curcuma longa L.), has been widely studied for its potential health benefits, including anti-inflammatory, antioxidant, and wound-healing properties. However, due to its low bioavail...
Curcumin, found in turmeric rhizomes (Curcuma longa L.), has been widely studied for its potential health benefits, including anti-inflammatory, antioxidant, and wound-healing properties. However, due to its low bioavailability and unfavorable pharmacokinetics, analogous compounds have been developed to obtain better biopharmaceutical characteristics and enhanced biological effects. In this study, we evaluated the activity of curcumin and three of its synthetic analogues (DMAD, DMAM, and RI75) on the viability and differentiation of a pre-osteoblastic cell line (MC3T3-E1). We also assessed the expression of key genes involved in tissue regeneration: vascular endothelial growth factor (vegf), stromal-derived growth factor 1 (SDF-1/CXCL12), and runt-related transcription factor 2 (runx2). The cells were treated with curcumin and the three analogues at concentrations of 10, 30, or 50 μM. All tested analogues and curcumin exhibited moderate to no cell toxicity compared to the cells treated under standard conditions across all concentrations after 24, 48, and 72 hours. Only the RI75 analogue showed upregulation of SDF-1, a crucial factor in tissue regeneration. Compared to curcumin, the DMAM and RI75 analogues also upregulated runx2 and vegf, both associated with osteodifferentiation. The RI75 analogue demonstrated greater mineralization than curcumin, and both promoted more nodule formation than the untreated control. Our data suggest that the curcumin analogue RI75 at 50 μM presents similar toxicity but enhanced biological activity compared to natural curcumin, making it a promising substance for material biomodifications.
Fractal analysis and assessment of lacunarity in mandibular osteoradionecrosis: a cross-sectional study with control group.
Barcelos NS, Brasileiro CB, Abreu LG … +5 more , Mendonça EF, Sousa-Neto SS, Sousa SF, Mesquita RA, Caldeira PC
Braz Oral Res · 2024 · PMID 39661795 · Full text
The objective of this study was to evaluate the fractal dimension (FD) and lacunarity of the mandibular bone, comparing patients with and without osteoradionecrosis (ORN). In a cross-sectional study with a control group,...
The objective of this study was to evaluate the fractal dimension (FD) and lacunarity of the mandibular bone, comparing patients with and without osteoradionecrosis (ORN). In a cross-sectional study with a control group, 25 patients were included and divided into a case group (with ORN, n = 14) and a control group (without ORN, n = 11). A digital panoramic radiograph taken after the end of radiotherapy (RT) was evaluated for each patient. FD and lacunarity of the mandibular bone were determined using ImageJ software. Descriptive, bivariate, and ROC curve analyses were performed. Cohen's d effect sizes were calculated. Significance was established at p < 0.05. The mean FD and lacunarity values were not significantly different between the groups. The area under the curve for FD and lacunarity were 0.579 and 0.661, respectively. The cut-off point for FD was ≤1.1714 and for lacunarity, > 0.3821, correctly classifying the majority of cases and controls. Most participants in the case group (63.6%) had a FD ≤ 1.1714 and the majority of participants in the control group (63.6%) had a FD >1.1714 (p = 0.395). For lacunarity, most individuals in the case group (72.7%) had a value > 0.3821 and most participants in the control group (63.6%) had a value ≤ 0.3821 (p = 0.198). In conclusion, the FD and lacunarity values did not show statistically significant differences between patients with and without ORN. However, the moderate and large magnitude of the effects seem to indicate that the results may be clinically relevant.
Mast cells and factor XIIIa+ dendrocytes in actinic cheilitis and lip squamous cell carcinoma.
Flores IL, de Arruda JAA, Abrantes TC … +10 more , Gamba TO, Abrahão AC, Anbinder AL, Ribeiro JL, Vasconcelos ACU, Andrade BAB, Aguiar MCF, Gomes APN, Abreu LG, Mesquita RA
Braz Oral Res · 2024 · PMID 39661794 · Full text
There is an interaction between dendrocytes and mast cells in the skin. However, in elastosis-related diseases such as actinic cheilitis (AC) and lower lip squamous cell carcinoma (LLSCC), this interaction remains unknow...
There is an interaction between dendrocytes and mast cells in the skin. However, in elastosis-related diseases such as actinic cheilitis (AC) and lower lip squamous cell carcinoma (LLSCC), this interaction remains unknown. We investigated the presence of intact and degranulated mast cells in AC and LLSCC. Associations of mast cells with factor XIIIa+ dendrocytes and inflammatory infiltrate were assessed. Forty cases of AC (20 with low-grade and 20 with high-grade epithelial dysplasia), 50 cases of LLSCC, and 10 cases of normal oral mucosa were evaluated. Toluidine blue staining was performed to identify mast cells, and mast cell densities were calculated in the inflammatory infiltrate. Factor XIIIa+ dendrocytes were immunohistochemically quantified. The highest ratio of intact/degranulated mast cells density was detected in LLSCC (5.9 cells/mm2), followed by AC with high-grade epithelial dysplasia (4.8 cells/mm2). Statistically significant differences were found in the density of intact mast cells compared to degranulated mast cells in AC with low-grade epithelial dysplasia (p<0.001), AC with high-grade epithelial dysplasia (p=0.005), and LLSCC (p<0.001). A positive correlation between degranulated mast cells and total inflammatory infiltrate (p=0.03) was observed in the LLSCC group. The expression of factor XIIIa+ dendrocytes was highest in AC with low-grade epithelial dysplasia (16.5 cells/mm2). The link between mast cell density, factor XIIIa+ dendrocytes, and inflammatory infiltrate indicates a potential crosstalk in lip carcinogenesis.
Ionizing radiation effects on osseointegration: a pre-clinical study.
Alves RO, Oliveira GJPL, Oliveira RC … +4 more , Limirio PHJO, Claudino M, Zanetta-Barbosa D, Soares PFB
Braz Oral Res · 2024 · PMID 39661793 · Full text
The purpose of this study was to evaluate the effect of a single dose of ionizing radiation (30 Gy) on the osseointegration of implants in the rabbit tibia. Twenty rabbits received two dental Morse-tapered junction impla...
The purpose of this study was to evaluate the effect of a single dose of ionizing radiation (30 Gy) on the osseointegration of implants in the rabbit tibia. Twenty rabbits received two dental Morse-tapered junction implants and one implant in each tibia. The animals were randomly divided into two groups (n=10), non-irradiated (NoIr) and irradiated (Ir), wherein the Ir group received a single dose of 30 Gy radiation 2 weeks after implant installation. Microtomographic analyses (BV/TV) and histomorphometric assessments (BIC and BABT) were performed 4 weeks after implant installation. One-way ANOVA, Tukey's test, and Student's t-test (α=0.05) were used for data analysis. The results showed that BV/TV did not differ significantly between the Ir and NoIr groups (P = 0.071). In the histomorphometric analysis, neither BIC nor BABT showed significant differences between the NoIr and Ir groups (p>0.05). In conclusion, ionizing radiation in dental implants does not appear to interfere with osseointegration when installed prior to irradiation.
Antimicrobial activity of PMMA enriched with nano-clay loaded with metronidazole and chlorhexidine.
Moffa EB, Malheiros SS, Silva LTS … +6 more , Branco DI, Grassia Junior RCF, Brandt WC, Goncalves F, Barao VAR, Boaro LCC
Braz Oral Res · 2024 · PMID 39661792 · Full text
Poly(methyl methacrylate) (PMMA) materials are highly susceptible to microbial colonization, predisposing patients to oral infections. To address this concern, we loaded PMMA samples with montmorillonite clay (MMT), a cr...
Poly(methyl methacrylate) (PMMA) materials are highly susceptible to microbial colonization, predisposing patients to oral infections. To address this concern, we loaded PMMA samples with montmorillonite clay (MMT), a crystalline nanoparticle, in combination with chlorhexidine (CHX) or metronidazole (MET) targeting improved antimicrobial action. PMMA samples were prepared with or without MMT loaded with either CHX or MET, establishing the following groups: control (acrylic resin without the addition of nanoparticles), MMT/CHX (acrylic resin with 5% by weight of MMT loaded with CHX), and MMT/MET (acrylic resin with 5% by weight of MMT loaded with MET). Mechanical properties such flexural strength, flexural modulus, and Knoop hardness were evaluated using a universal testing machine. Antimicrobial efficacy was assessed via agar diffusion tests against Enterococcus faecalis and Porphyromonas gingivalis. The addition of MMT loaded with CHX did not affect the flexural strength and flexural modulus of PMMA compared to the control group (p > 0.05). However, MMT/MET reduced all mechanical properties of PMMA (p < 0.05). Both loaded-PMMA materials demonstrated antibacterial activity against E. faecalis but not against P. gingivalis. In conclusion, the incorporation of MMT/CHX into acrylic resin appears to be the most promising approach to combat microbial colonization while preserving PMMA mechanical properties. Future research should focus on optimizing material characteristics to enhance antimicrobial properties, paving the way for clinical applicability.
Global quality scores of Brazilian public health system-related YouTubeTM videos and their users' engagement.
Silva EMM, Camargos CR, Pordeus IA … +3 more , Abreu MHNG, Vargas-Ferreira F, Mattos FF
Braz Oral Res · 2024 · PMID 39661791 · Full text
The study assessed the Global Quality Score (GQS) and informational engagement of users with YouTube videos on the Brazilian public health system (SUS). The YouTube video search tool was used with the Portuguese keywords...
The study assessed the Global Quality Score (GQS) and informational engagement of users with YouTube videos on the Brazilian public health system (SUS). The YouTube video search tool was used with the Portuguese keywords 'unified health system' and 'SUS'. The first 100 videos returned in the search were studied, using the GQS to measure their educational value, usefulness, and information quality. Users' engagement with the videos was calculated based on their number of likes/reactions and comments. Other data collected were authorship, year of publication, topic approached, target audience, video length, and use of references. Two trained and calibrated researchers collected the data. Multiple analysis was performed with Logistic Regression, using a 95% confidence interval and significance of p<0.05. There were no poor or generally poor GQS scores (scores 1 and 2) and most videos (58%) achieved moderate or good scores (scores 3 and 4). Videos published after the onset of COVID-19 had a 70% lower chance of engagement than those published in pre-pandemic years (OR: 0.30; 95%CI: 0.12-0.74). Videos that targeted healthcare professionals were 72% less likely to achieve higher GQS scores, than those with an unidentified target audience (OR: 0.28; 95%CI: 0.10-0.75). The informational engagement of the videos showed fewer comments than likes/reactions. Most YouTube videos about the SUS had moderate or good global quality, which was associated with their period of publication and choice of target audience.
Tissue response and expression of interleukins (IL)-1ß, IL-6, IL-10 after pulp capping with bioglasses in mice.
Chaves HGDS, Figueiredo B, Maia CA … +9 more , Reis-Prado AHD, Antunes MM, Mesquita RA, Tavares WLF, Menezes GB, Diniz IMA, Crovace MC, Avelar GF, Benetti F
Braz Oral Res · 2024 · PMID 39661790 · Full text
This study aimed to evaluate the pulp response to F18 and cobalt-doped F18 bioglass (F18Co) in comparison with calcium hydroxide (CH) after pulp capping. The maxillary first molars of 48 rats were divided into F18, F18Co...
This study aimed to evaluate the pulp response to F18 and cobalt-doped F18 bioglass (F18Co) in comparison with calcium hydroxide (CH) after pulp capping. The maxillary first molars of 48 rats were divided into F18, F18Co, CH, and control (no intervention) groups. The pulp was exposed, the materials were placed, and the teeth were capped. After 7 and 15 days, the animals were euthanized for pulp evaluation and interleukin (IL) expression determination. Statistical analysis was carried out using the SigmaPlot® program (Systat Software Inc., for Windows, version 12.0). The data obtained in the analyses were subjected to the non-parametric Kruskal-Wallis test, followed by Dunn's test. For all tests, statistical significance was set at p < 0.05. The CH group exhibited mild to moderate inflammation, whereas the bioglass groups displayed moderate to severe inflammation, indicating a notable difference between the control and bioglass groups. At 7 days, both the CH and most of the bioglass specimens showed moderate disorganization. On day 15, CH displayed mildto-moderate disorganization, whereas F18 and F18Co exhibited significantly more moderate-to-severe disorganization. There were no significant differences in IL-6 and IL-10 expressions between groups at 7 days, but a noteworthy increase in IL-1β was observed in both CH and F18. After 15 days, there was a greater expression of IL-6 and IL-1β in the bioglass groups. No significant IL-10 expression was observed. Bioglass performed less effectively than CH when in direct contact with the pulp tissue.
Intraoral appliance treatment modulates inflammatory markers and oxidative damage in elderly with sleep apnea.
Peres A, Rosa JCFD, Ribeiro JS … +4 more , Silva SL, Bündchen C, Dornelles GP, Fontanella V
Braz Oral Res · 2024 · PMID 39661789 · Full text
Obstructive sleep apnea (OSA) causes intermittent hypoxia, increased production of reactive oxygen species, and inflammation, which may elevate morbidity and mortality from cardiovascular disease. The objective of the pr...
Obstructive sleep apnea (OSA) causes intermittent hypoxia, increased production of reactive oxygen species, and inflammation, which may elevate morbidity and mortality from cardiovascular disease. The objective of the present study was to investigate the effect of the intraoral appliance (IOA) as a treatment for OSA when it comes to the modulation of inflammatory markers and oxidative damage in elderly individuals. This "before and after" clinical trial included 9 patients diagnosed with OSA recruited from a multicenter randomized clinical trial study that evaluated the treatment with IOA for 60 days. Demographic and anthropometric variables, apnea and hypopnea index (AHI), and oxygen desaturation index (ODI) were collected by type III polysomnography, Epworth Sleepiness Scale, and inflammatory and oxidative damage markers (interleukin 6 (1L-6); tumor necrosis factor α (TNF-α); interleukin 10 (IL-10); thiobarbituric acid reactive substances (TBARS); total thiols; advanced oxidation protein products (AOPP), and nitric oxide (NO)). Shapiro-Wilk test, paired t-test and Pearson's correlation tests were used to analyze the results, respectively (α=0.05). The sample had a mean age of 71.86 ± 4.63 years, the majority were women (55.55%), and had a significant reduction in AHI (p=0.003), ODI (p=0.038), IL-10 (p=0.0001), AOPP (p=0.038) and TBARS levels (p=0.0001). There was a significant correlation between IL-10 and NO (r=0.855) and between TBARS and IL-6 (r=0.669), both after treatment. This study demonstrated that treating elderly patients with OSA using an IOA for 60 days reduces oxidative damage through the modulation of AOPP and TBARS.
Municipality-level characteristics associated with very good quality water fluoridation in Brazil in 2018.
Belotti L, Paulino CM, Frazão P
Braz Oral Res · 2024 · PMID 39661788 · Full text
The aim of this study was to analyze factors associated with the quality of fluoridation in water supply systems in Brazil in 2018. An ecological study was conducted using official data sources on fluoridation provision...
The aim of this study was to analyze factors associated with the quality of fluoridation in water supply systems in Brazil in 2018. An ecological study was conducted using official data sources on fluoridation provision and fluoride concentration surveillance in 2018. Inclusion criteria were municipalities with a water supply system and accurate data. Municipalities meeting the quality standard, defined as having 80% or more of water samples within the optimal level for caries prevention were classified as "very good" fluoridation quality. Independent variables encompassed structural aspects, socioeconomic factors, and indicators of managerial and technical capabilities in water surveillance. Prevalence ratios were estimated using Poisson regression with robust variance. A total of 58.9% of municipalities with water supply systems had fluoride-adjusted water, with 65.3% adequately contributing to the surveillance data system. Among these, 42.2% exhibited "very good" fluoridation quality. Quality was higher in larger municipalities with elevated socioeconomic indexes and per capita expenditure on sanitary surveillance above the median. Additionally, municipalities with a conformity rate of free residual chlorine concentration of 75% or higher had better fluoridation quality. After adjusting for all variables, fluoridation quality was lower in municipalities with above-median per capita gross domestic product, higher socioeconomic inequality, and below-median monthly household per capita income. Fluoridation quality was linked to municipality-level characteristics concerning structural aspects, socioeconomic factors, and managerial and technical factors in water surveillance. The information generated regarding the coverage features of surveillance and fluoridation strategies can be highly valuable for redirecting inter-sectoral public policy management.
Effect of final irrigation protocols on the structural integrity and mechanical properties of the root dentine.
Savaris JM, Dotto MEP, Garcia LDFR … +4 more , Silva EJNLD, Henriques BAPC, Teixeira CDS, Bortoluzzi EA
Braz Oral Res · 2024 · PMID 39661787 · Full text
This study compared the effects of different final irrigation protocols on the mechanical properties and structural integrity of root dentine. One-hundred eight teeth were instrumented and irrigated with sodium hypochlor...
This study compared the effects of different final irrigation protocols on the mechanical properties and structural integrity of root dentine. One-hundred eight teeth were instrumented and irrigated with sodium hypochlorite (NaOCl) using conventional irrigation (CI). Teeth were distributed into four groups based on final irrigation protocols: Control Group (CG): 17%EDTA/CI + H2O; G1: 2.5%NaOCl/Passive Ultrasonic Irrigation (PUI) + EDTA/PUI + NaOCl/PUI (60s each); G2: EDTA/PUI + NaOCl/PUI (30s each); G3: EDTA/PUI + NaOCl/PUI + H2O/PUI + CHX/PUI (30s each). Four tests were conducted: three-point flexural strength test, Scanning Electron Microscopy analysis, microhardness assessment, and the push-out bond strength (POBS) of the filling material to the root dentine. Data were analyzed using ANOVA and Tukey tests (Flexural strength), and Student t-test (Microhardness). Erosion scores and POBS were analyzed by Kruskal-Wallis and Dunn tests (α = 0.05). Results indicated no significant differences in flexural strength (p > 0.05) among groups. CG exhibited the lowest erosion scores in the cervical third. In the middle third, CG had lower scores than G1 and G2, while in the apical third, CG had lower scores than G1 (p < 0.05). Microhardness values decreased following the protocols (p < 0.05), except for the CG (p > 0.05). G2 displayed higher POBS values in the middle and apical thirds (p < 0.05). The protocols did not significantly influence the flexural strength of root dentine. However, they did affect microhardness and promoted greater erosion. The best results for POBS were observed when the final irrigation involved the sequential use of EDTA and NaOCl employing PUI for 30 seconds in each solution.
Self-perceived oral health among Brazilian university students: a cross-sectional study.
Costa LBA, Cunha RO, Leite ICG
Braz Oral Res · 2024 · PMID 39661786 · Full text
This cross-sectional study aimed to analyze the self-perceived oral health of young university students at the Federal University of Juiz de Fora and identify the associated factors. Data were collected in 2021 using a s...
This cross-sectional study aimed to analyze the self-perceived oral health of young university students at the Federal University of Juiz de Fora and identify the associated factors. Data were collected in 2021 using a self-administered questionnaire containing questions on students' sociodemographic variables and oral health. Binary logistic regression was applied in the multivariate analysis using the SPSS (Statistical Package for the Social Sciences) software (version 20.0) for Windows. The final analysis included 1,316 students aged 17-24 years. The prevalence of negative self-perception of oral health was 14.1% (95%CI: 12.2-16.0). The following variables were associated with negative self-perception of oral health: single marital status (OR = 0.34; 95%CI: 0.12-0.98), monthly family income of up to three minimum wages (OR = 2.02; 95%CI: 1.32-3.09), non-regular use of dental services (OR = 2.29; 95%CI: 1.48-3.53), dissatisfaction with the last service (OR = 1.97; 95%CI: 1.23-3.16), fear of dental treatment (OR = 1.56; 95%CI: 1.06-2.29), dissatisfaction with the appearance of teeth and mouth (OR = 5.27; 95%CI: 3.37-8.22), and perceived need for dental treatment (OR = 6.94; 95%CI:3.14-15.33). In conclusion, most young university students had a positive self-perception of oral health. However, factors related to socioeconomic profile, access to oral health services, and satisfaction with one's appearance were found to increase the likelihood of having a negative self-perception of oral health.
Mouth breathing is associated with a higher prevalence of anterior dental caries in preschool children.
Soares MEDC, Ramos-Jorge J, Lima LJS … +4 more , Moreira LV, Fernandes IB, Ramos-Jorge ML, Galo R
Braz Oral Res · 2024 · PMID 39661785 · Full text
This cross-sectional study aimed to investigate the association between anterior dental caries and the predominant type of breathing in preschoolers. The research involved a sample of 257 children aged between 3 and 5 ye...
This cross-sectional study aimed to investigate the association between anterior dental caries and the predominant type of breathing in preschoolers. The research involved a sample of 257 children aged between 3 and 5 years, who were enrolled in public daycare centers and preschools in the city of Diamantina, MG, Brazil. A questionnaire was applied to parents/guardians to collect sociodemographic data, habits, oral health, and a dietary diary used to calculate the Sucrose Consumption Index. The predominant type of breathing, whether nasal or through the mouth, was determined through direct observation. Dental caries was assessed using the International Caries Detection and Assessment System (ICDAS). Descriptive analyses and the Poisson regression were conducted to analyze the data. The results revealed a higher prevalence of anterior dental caries among children who predominantly breathed through the mouth (PR = 1.57; 95%CI: 1.01-2.46; p = 0.047), who exhibited a higher frequency of sucrose consumption (PR = 4.02; 95%CI: 2.03-7.95; p < 0.001), and had mothers with lower educational levels (PR = 1.53; 95%CI: 1.01-2.32; p = 0.043). Pacifier use was associated with a lower prevalence of anterior dental caries (PR = 0.57; 95%CI: 0.34-0.96; p = 0.035). In conclusion, this investigation demonstrated an association between carious lesions in the anterior teeth and predominantly mouth breathing in preschoolers.
Fatigue behavior of sintered, glazed and glass-infiltrated surfaces of 5Y-PSZ bonded plates.
Silva ACD, Ortiz LPN, Alves LMM … +6 more , Dapieve KS, Campos TMB, Bottino MA, Thim GP, Valandro LF, Marinho RMM
Braz Oral Res · 2024 · PMID 39661784 · Full text
This study evaluated the effect of different occlusal surface finishes (glaze and silica glass infiltration) on surface characteristics and fatigue behavior of partially stabilized zirconia (PSZ) plates adhesively bonded...
This study evaluated the effect of different occlusal surface finishes (glaze and silica glass infiltration) on surface characteristics and fatigue behavior of partially stabilized zirconia (PSZ) plates adhesively bonded onto epoxy resin discs. PSZ disc specimens (n = 15; Katana blocks STML, Kuraray Noritake Dental) were produced (Ø = 10 mm; thickness = 1.2 mm) and allocated into 3 groups: As sintered (S), silica glass infiltration (SGI), and glaze application (G). The PSZ intaglio surface was air-abraded with 50-µm alumina powder followed by bonding agent application. All produced PSZ were adhesively cemented onto dentin analogue discs made of epoxy resin material (Ø = 10 mm; thickness = 2 mm). Step stress fatigue test was performed (load ranging from 200 to 1800 N; step size 100 N and 10,000 cycles; 20 Hz). The topographic, microstructural, and fractographic analyses were performed by scanning electron microscopy. Results: No statistically significant difference in fatigue behavior was detected among the groups. All failures started at the bonding surface. Silica glass-infiltration and glaze layer application provided a smoothing effect, while the sintered group had a surface with grooves. The occlusal surface finishing method (silica glass infiltration or glazing) had no deleterious effect on fatigue behavior of adhesively bonded PSZ plates.
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