Nan Fang Yi Ke Da Xue Xue Bao = Journal Of Southern Medical University[JOURNAL]
Sun
200 papers
[Angio-associated migratory cell protein is highly expressed in hepatocellular carcinoma and promotes tumor cell invasion and metastasis].
Li C, Yin G, Cheng X … +1 more , Jiang Y
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429616 · Full text
OBJECTIVES: To investigate the clinical significance of abnormal expression of angio-associated migratory cell protein (AAMP) in hepatocellular carcinoma (HCC). METHODS: Bioinformatics analyses were used to analyze AAMP...
OBJECTIVES: To investigate the clinical significance of abnormal expression of angio-associated migratory cell protein (AAMP) in hepatocellular carcinoma (HCC). METHODS: Bioinformatics analyses were used to analyze AAMP expression level in HCC and its prognosis value. In 60 pairs of HCC and adjacent tissues, AAMP expression was detected immunohistochemically and its correlation with clinicopathological characteristics of the patients was analyzed. In cultured Mahlavu and Huh-7 cells with lentivirus-mediated AAMP knockdown, the changes in cell proliferation, apoptosis, migration and invasion were observed, and their lung metastasis following tail vein injection in nude mice were assessed. In HCC cells with AAMP knockdown, Western blotting, immunofluorescence staining or RT-qPCR were used to examine the changes in expression levels of E-cadherin, N-cadherin, Vimentin and Snail and the effects of MG-132 and CHX on RhoA expression. The correlation between the expressions of AAMP and RhoA in HCC tissues was analyzed by immunohistochemistry. RESULTS: Bioinformatics analysis showed that AAMP expression was elevated in HCC tissues (<0.05) in correlation with advanced clinical stage and poor prognosis (<0.05). Immunohistochemistry results confirmed significant correlations of high AAMP expression with Edmondson-Steiner grade (III+IV), venous infiltration and TNM stage (III+IV) of HCC (<0.05). In cultured HCC cells, AAMP knockdown did not significantly affect cell proliferation or apoptosis, but obviously suppressed cell migration and invasion and lung metastasis in nude mice. AAMP knockdown significantly increased E-cadherin expression, decreased N-cadherin, Vimentin and Snail expressions, and reduced RhoA protein levels without obviously affecting RhoA mRNA levels. MG-132 treatment blocked the inhibitory effect of AAMP knockdown on RhoA protein expression. The expressions of AAMP and RhoA showed a significant positive correlation in HCC tissues (<0.05). CONCLUSIONS: AAMP overexpression is associated with malignant clinical features of HCC and promotes epithelial-mesenchymal transition and metastasis of HCC cells partly by stabilizing RhoA expression.
[Preoperative serum magnesium as a biomarker for predicting delirium following non-cardiac surgery in elderly patients: a retrospective cohort study].
Yuan M, Ruan X, Li Y … +8 more , Zhang T, Hao C, Li H, Lou J, Cao J, Liu Y, Mi W, Zhang X
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429615 · Full text
OBJECTIVES: To investigate the association of preoperative serum magnesium (sMg) level with postoperative delirium (POD) in elderly surgical patients and the mediating role of systemic inflammation. METHODS: This retrosp...
OBJECTIVES: To investigate the association of preoperative serum magnesium (sMg) level with postoperative delirium (POD) in elderly surgical patients and the mediating role of systemic inflammation. METHODS: This retrospective cohort study was conducted among 12 876 patients aged ≥65 years undergoing non-cardiac, non-neurological surgeries at Chinese PLA General Hospital between January, 2014 and December, 2021. Preoperative sMg and C-reactive protein (CRP) levels were measured within 30 days before surgery. POD was identified within 7 days postoperatively using structured chart review based on the Confusion Assessment Method. Multivariate logistic regression and restricted cubic spline (RCS) models were used to evaluate the association between sMg and POD. Mediation analysis with structural equation modeling was used to quantify the indirect effect of CRP after adjusting for the confounding factors. RESULTS: POD was identified in 685 (5.3%) of the patients. A significant nonlinear association was observed between preoperative sMg levels and POD risk, and POD incidence was the lowest in patients with sMg levels of 0.90-0.94 mmol/L. Compared with those in the 4th quintile, the patients in the lowest quintile exhibited a markedly increased risk of POD (OR=1.81, 95% : 1.41-2.35) even after adjustment for multiple confounding factors. Mediation analysis suggested that CRP explained 17.1% of the total effect of sMg on POD risk, and a stronger mediating effect was observed in cancer as compared with the non-cancer patients (24.1% 11.9%). Subgroup analyses revealed a significant nonlinear relationship between sMg and POD particularly in cancer patients and patients beyond 75 years of age. CONCLUSIONS: Preoperative sMg level is independently associated with an increased POD risk in elderly patients, mediated partly by systemic inflammation. sMg may serve as a modifiable biomarker for early risk stratification and prevention for POD in perioperative care.
[Knockdown of inhibits mitochondrial function and mRNA mA modification and expression of key genes in mouse hepatocytes].
Ding S, Liao Y, Bai X … +2 more , Huang J, Asakawa T
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429614 · Full text
OBJECTIVES: To investigate the role of gene in regulating mitochondrial function and mRNA mA modification and expressions of the key genes in mouse hepatocytes. METHODS: In mouse hepatocyte AML12 cells, the effects of l...
OBJECTIVES: To investigate the role of gene in regulating mitochondrial function and mRNA mA modification and expressions of the key genes in mouse hepatocytes. METHODS: In mouse hepatocyte AML12 cells, the effects of lentivirus-mediated knockdown and transfection with a negative control virus on mitochondrial membrane potential and mitochondrial respiratory function were analyzed using the TMRE fluorescent probe and the Seahorse extracellular flux analysis system. Methylated RNA immunoprecipitation (MeRIP) combined with mA microarray was utilized to evaluate the changes in mRNA mA modification and expression levels, followed by enrichment analysis to identify the functionally relevant genes. The mA modification and expression levels of the mRNAs were validated by qPCR. RESULTS: Compared with the negative control group, the cells with knockdown exhibited significantly reduced mitochondrial membrane potential and respiratory capacity. mA microarray analysis revealed significant changes in mA modification levels (fold change>1.5) of 7814 mRNAs, including 152 upregulated and 7662 downregulated mRNAs. Integrated expression analysis identified 2497 mRNAs showing coordinated changes in mA modification and expression levels. These mRNAs were enriched in the oxidative phosphorylation pathway, with , , and exhibiting the highest percent changes in mA modification. Both microarray and qPCR results showed that the mA modification and expression levels of , and mRNAs were significantly reduced in cells with knockdown compared to the NC group. CONCLUSIONS: knockdown induces significant alterations in mRNA mA modification as well as their expression levels. The regulatory effects of 1 on mitochondrial function may be mediated by modulation of mA modification of , , and mRNAs.
[Overwork damages myocardial energy metabolism homeostasis in mice].
Cui J, Lai R, Chen S … +4 more , Qu S, Liao Y, Ma X, Li Y
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429613 · Full text
OBJECTIVES: To investigate the effect of overwork on myocardial energy metabolism in mice. METHODS: Thirty-two C57BL/6J mice were randomized equally into a control group and 3 overwork groups with overwork for 2, 4, and...
OBJECTIVES: To investigate the effect of overwork on myocardial energy metabolism in mice. METHODS: Thirty-two C57BL/6J mice were randomized equally into a control group and 3 overwork groups with overwork for 2, 4, and 6 weeks (W2, W4, and W6 groups, respectively). The mice in overwork groups were subjected to daily forced water standing and restraint. The changes in body weight and general condition of the mice were observed weekly. After successful modeling, the mice were examined for changes in echocardiography, blood glucose/lipid profiles, myocardial pathologies, myocardial TG and ATP levels, and expressions in CD36, GLUT1, CPT1B, PPARα, PFKM, and PKM2 using immunohistochemistry, RT-qPCR or Western blotting. RESULTS: The mice with prolonged overwork exhibited reduced activity with hair loss, dull fur, and slowed body weight gain without significant changes in cardiac index or function. Blood glucose levels increased significantly in W2 and W4 groups but decreased in W6 group. Serum TG level increased significantly while TC, HDL, and LDL decreased in W4 and W6 groups. HE staining revealed myocardial swelling, disorganization, and vacuolation in the mouse models. Myocardial TG was elevated in W4 and W6 groups and ATP level decreased in W6 group. The mRNA and protein expressions of CPT1B and PPARα were downregulated in W4 and W6 group, and CD36 expression increased significantly in W4 group. GLUT1 and PFKM/PKM2 expressions decreased obviously in W2 group but increased in W4 and W6 group compared with that in W2 group. CONCLUSIONS: Short-term overwork causes elevation of blood glucose and suppresses glycolysis in mice, while prolonged overwork reduces glucose, increases TG, impairs fatty acid oxidation, and limits glycolytic compensation to eventually result in myocardial damage, lipid accumulation, and ATP deficiency.
[ Decoction alleviates renal fibrosis in rats by ameliorating oxidative stress and inflammation through the Rap1/MAPK/FoxO3a signaling pathway].
Lu X, Liu Z, Liu Y … +4 more , Pang T, Bian R, Guo L, He X
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429612 · Full text
OBJECTIVES: To explore the mechanism of Decoction (SQXZD) for improving renal fibrosis (RF) in rats. METHODS: The chemical components of SQXZD were identified using UPLC-Q Exactive/MS, and component-disease target netwo...
OBJECTIVES: To explore the mechanism of Decoction (SQXZD) for improving renal fibrosis (RF) in rats. METHODS: The chemical components of SQXZD were identified using UPLC-Q Exactive/MS, and component-disease target network and enrichment analyses were conducted to screen the key pathways and targets. In the animal experiment, 49 male SD rats were randomized equally into blank control group, sham operation group, unilateral ureteral obstruction-induced RF model group, losartan treatment (daily dose 4.6 mg/kg) group, and low-, medium-, and high-dose SQXZD (9.7, 19.4, and 38.8 g/kg, respectively) treatment groups. After 14 days' treatment, renal pathologies and collagen deposition of the rats were examined with HE and Masson staining, and serum levels of BUN, Cr, SOD, MDA, GSH-px, IL-6, and TNF-α were detected. Western blotting and qRT-PCR were used to detect renal protein and mRNA expressions of α‑SMA, Col-I, NAKED2, Rap1, B-raf, Raf-1, MEK3/6, p38MAPK, MEK, ERK1/2, p-ERK1/2, FoxO3a, p-FoxO3a, and MnSOD. RESULTS: A total of 263 chemical components were identified in SQXZD. Network pharmacology revealed 170 intersecting targets between the components and RF enriched in the MAPK, Rap1, and FoxO pathways. The rat models of RF showed abnormal renal structural changes, increased fibrosis area, elevated serum BUN, Cr, MDA, IL-6, and TNF-α levels, reduced SOD and GSH-px levels, upregulated renal expressions of α‑SMA, Col-I, NAKED2, Rap1, B-raf, MEK, ERK1/2, p-ERK1/2, MEK3/6, and p38MAPK, and downregulated Raf-1, FoxO3a, p-FoxO3a, and MnSOD expressions. Treatment with losartan and SQXZD (especially at the medium dose) obviously lessened renal pathologies, improved renal functions, alleviated oxidative stress and inflammation, and ameliorated abnormal changes in the Rap1/MAPK/FoxO3a signaling pathway in the rat models. CONCLUSIONS: SQXZD alleviates RF and improves renal function in rats possibly by ameliorating renal oxidative stress and inflammation regulating the Rap1/MAPK/FoxO3a signaling pathway.
[Acupuncture alleviates chronic airway inflammation in obese asthmatic mice by downregulating Vnn1 and FAM126B].
Li X, Ye T, Xi L … +2 more , Li C, Liu H
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429611 · Full text
OBJECTIVES: To identify the differentially expressed genes in obese asthma versus non-obese asthma and evaluate the effect of acupuncture on chronic airway inflammation in obese mice with asthma. METHODS: The key genes o...
OBJECTIVES: To identify the differentially expressed genes in obese asthma versus non-obese asthma and evaluate the effect of acupuncture on chronic airway inflammation in obese mice with asthma. METHODS: The key genes of obesity-related asthma were screened using GSE110551 dataset from the GEO database, and the characteristic genes were selected from the genes with the highest correlation with T cells using Lasso regression and SVM feature selection algorithms. Fifty C57BL/6J mice (5-6 weeks old) were randomized equally into 5 groups, including a normal feeding (control) group, a high-fat feeding group, and 3 high-fat feeding and ovalbumin sensitization groups with intraperitoneal injections with saline or dexamethasone (DEX), or treated with acupuncture. Western blotting, qPCR, and flow cytometry were used to analyze the changes in the expressions of the key genes and inflammation in the airway of the mice. RESULTS: FAM126B and VNN1 were identified as the characteristic genes in obesity-related asthma for subsequent analysis. The mice with high-fat feeding and ovalbumin sensitization showed the highest expression levels of Vnn1 and FAM126B among the 5 groups, with also significantly decreased Treg cell percentage and obvious inflammatory cell infiltration in the lungs. Treatment with DEX and acupuncture both significantly decreased the number of infiltrating inflammatory cells and increased the percentage of Treg cells in airway of the mouse models of obesity-related asthma. HIF-1α was identified as a key regulatory factor for asthmatic inflammation, and its expression level was significantly increased in the asthmatic mouse models but obviously lowered after acupuncture treatment or dexamethasone therapy. CONCLUSIONS: Vnn1 and FAM126B may serve as the key therapeutic targets for treatment of obese asthma patients. Acupuncture treatment may downregulate airway HIF-1α by reducing the expressions of Vnn1 and FAM126B and increasing the number of Treg cells.
[Kahweol improves motor function of mice with spinal cord injury by inhibiting microglial activation regulating the IκBα/NF-κB pathway].
Xia J, Chen Y, Ren L … +4 more , Li J, Song X, Tao L, Hu J
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429610 · Full text
OBJECTIVES: To investigate the mechanism of kahweol for promoting motor function recovery in mice with spinal cord injury (SCI). METHODS: Fifty-four 8- to 10- week-old C57BL/6J mice were randomized equally into sham oper...
OBJECTIVES: To investigate the mechanism of kahweol for promoting motor function recovery in mice with spinal cord injury (SCI). METHODS: Fifty-four 8- to 10- week-old C57BL/6J mice were randomized equally into sham operation (laminectomy only) group, SCI group (laminectomy with spinal cord contusion), and Kahweol treatment group (with daily intraperitoneal injection of 20 mg/kg Kahweol following SCI). Motor function of the mice was evaluated using BMS scores, footprint analysis, and swimming test, and SCI area, myelin integrity, and neuron survival were assessed using HE, LFB, and Nissl staining. In a co-culture system of lipopolysaccharide (LPS)‑stimulated BV2 cells and HT22 neurons, the effects of different concentrations of Kahweol and PMA, a NF-κB pathway activator, on the number of activated microglia and apoptotic neurons were evaluated with immunofluorescence staining, and the changes in apoptosis-related proteins and IκBα/NF‑κB pathway proteins were detected using Western blotting. The levels of inflammatory factors (TNF-α, IL-6, and IL-1β) were measured by qRT-PCR and ELISA. RESULTS: In the mice with SCI, kahweol treatment significantly promoted motor function recovery, reduced injury area in the spinal cord tissue, and increased the myelinated area and number of neurons. In both the mouse models and the cell co-culture system, kahweol treatment effectively alleviated neuronal apoptosis by inhibiting microglial activation and reducing the release of inflammatory factors. The results of Western blotting showed that kahweol significantly decreased the phosphorylation levels of NF‑κB and IκBα. In the cell co-culture system, PMA obviously attenuated the inhibitory effect of kahweol on BV2 cell activation and neuronal apoptosis. CONCLUSIONS: Kahweol promotes motor function recovery of mice with SCI by suppressing microglial activation inhibiting the NF‑κB pathway, which shed light on a new strategy for clinical treatment of SCI.
[SERPINE1 overexpression promotes proliferation and paclitaxel resistance of triple-negative breast cancer cells by inducing M2 macrophage polarization].
Zhang Q, Liu B, Lei L … +8 more , Wang Y, Zhang X, Mao Z, Tang P, Zhang J, Yang J, Peng Y, Liu Z
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429609 · Full text
OBJECTIVES: To investigate the regulatory effect of Serpin Family E Member 1 (SERPINE1) on immune microenvironment and paclitaxel (PTX) resistance of triple-negative breast cancer (TNBC) cells. METHODS: CCK-8 assay was u...
OBJECTIVES: To investigate the regulatory effect of Serpin Family E Member 1 (SERPINE1) on immune microenvironment and paclitaxel (PTX) resistance of triple-negative breast cancer (TNBC) cells. METHODS: CCK-8 assay was used to determine the half-maximal inhibitory concentration of PTX in TNBC cell line MDA-MB-231. In wild-type MDA-MB-231 cells and a PTX-resistant MDA-MB-231 cell line (MDA-MB-231/PTX) established by stepwise increasing low-dose PTX treatment, the effects of Western blot-verified transfection with SERPINE1 overexpression plasmids or SERPINE1 siRNAs on cell apoptosis were evaluated using Hoechst 33258 staining and by detecting expression levels of cleaved caspase-3 using Western blotting. The changes in proliferation of the transfected cells were assessed using EdU and CCK-8 assays. The breast cancer cells with different treatments were co-cultured with macrophages, and M1 and M2 polarization of the macrophages were analyzed with flow cytometry and Western blotting. In nude mouse models bearing subcutaneous breast cancer cell xenografts, the effects of SERPINE1 overexpression and knockdown in the engrafted cells on tumor growth and PTX resistance were evaluated. RESULTS: SERPINE1 overexpression significantly inhibited apoptosis and promoted proliferation of MDA-MB-231 cells, and SERPINE1 knockdown obviously promoted apoptosis and inhibited proliferation of MDA-MB-231/PTX cells. The macrophages co-cultured with SERPINE1-overexpressing breast cancer cells showed enhanced M2 polarization and suppressed M1 polarization with a lowered M1/M2 ratio. In the tumor-bearing nude mouse models, SERPINE1 overexpression in the engrafted cells resulted in significantly accelerated tumor growth. CONCLUSIONS: In MDA-MB-231 cells, SERPINE1 overexpression promotes cell proliferation, inhibits apoptosis, and enhances PTX resistance. SERPINE1 plays a regulatory role in macrophage polarization in the immune microenvironment of breast cancer, and its high expression promotes M2 polarization of the macrophages.
[ polysaccharide delays osteoarthritis mouse chondrocyte degeneration by modulating the glycolysis-pyroptosis axis targeting the lncRNA XIST].
Fu C, Chen R, Xu S … +3 more , You J, Lin Q, Huang Y
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429608 · Full text
OBJECTIVES: To investigate the mechanism by which polysaccharide (MOP) delays osteoarthritis chondrocyte degeneration. METHODS: In primary cultures of chondrocytes from 4-week-old C57BL/6 mice, the effects of IL-1β and...
OBJECTIVES: To investigate the mechanism by which polysaccharide (MOP) delays osteoarthritis chondrocyte degeneration. METHODS: In primary cultures of chondrocytes from 4-week-old C57BL/6 mice, the effects of IL-1β and MOP treatment at different concentrations on cell viability were assessed with CCK-8 assay. The treated cells were examined for protein expressions of PKM2, caspase-1, and GSDMD using Western blotting and for XIST expression using fluorescence hybridization (FISH). In IL-1β-induced mouse chondrocytes, the effects of MOP, transfection for XIST overexpression or knockdown, and MOP treatment after the transfection were tested by detecting mRNA levels of GluT1, HK2, PKM2, LDHA, PFKFB3, NLRP3, caspase-1, and GSDMD; flow cytometry, Western blotting, and toluidine blue staining were used to analyze chondrocyte apoptosis, expressions of glycolysis and pyroptosis regulators, and glycosaminoglycan expression. RESULTS: The second-passage chondrocytes showed good viability and positive collagen II staining. IL-1β induction caused degenerative morphological changes of the cells, decreased collagen II expression, and upregulated cellular expressions of PKM2, caspase-1, and GSDMD proteins. MOP treatment (especially at 4 mg/mL) significantly enhanced cell viability and reduced HK2, PKM2, caspase-1 and GSDMD expressions in IL-1β‑induced mouse chondrocytes. XIST was localized predominantly in the nuclei of the chondrocytes, and its expression increased significantly in IL-1β‑treated cells, and was attenuated by MOP treatment. XIST overexpression synergized with IL-1β to upregulate mRNA and protein expressions of glycolysis- and pyroptosis-related factors in the chondrocytes, and such effects were obviously attenuated by MOP. Conversely, XIST knockdown significantly inhibited chondrocyte apoptosis and glycosaminoglycan expression, and down-regulated glycolysis- and pyroptosis-related proteins. MOP treatment exhibited similar protective effects to XIST knockdown, and their combination significantly augmented these protective effects. CONCLUSIONS: MOP mitigates IL-1β‑induced mouse chondrocyte degeneration by modulating glycolysis and pyroptosis via targeting XIST.
Protective effect of Lonicerae Japonicae Flos extract against doxorubicin-induced myocardial injury in mice and the possible mechanisms.
Xia S, Wei H, Hong W … +6 more , Zhang Y, Yin F, Zhang Y, Zhang L, Gao Q, Ye H
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Dec · PMID 41429607 · Full text
OBJECTIVES: To evaluate the protective effect of Lonicerae Japonicae Flos (LJF) extract against doxorubicin (DOX)-induced cardiotoxicity (DIC) and explore the possible mechanisms. METHODS: Network pharmacology, bioinform...
OBJECTIVES: To evaluate the protective effect of Lonicerae Japonicae Flos (LJF) extract against doxorubicin (DOX)-induced cardiotoxicity (DIC) and explore the possible mechanisms. METHODS: Network pharmacology, bioinformatics analysis and molecular docking were used to predict the targets of the core components of LJF. In a mouse model of DOX-induced myocardial injury, the protective effects of different doses of LJF extract were evaluated and the underlying mechanisms were explored by detecting the changes in mouse myocardial functions, myocardial enzymes, myocardial pathologies, and the expressions of inflammatory factors and pyroptosis-related proteins. RESULTS: The 10 core ingredients of LJF showed strong binding to AKT, EGFR, and GSK3β. In the animal experiment, the DOX-treated mice, compared with the sham-treated mice, had significantly decreased cardiac output, stroke volume, left ventricular ejection fraction, left ventricular fraction shorting, elevated serum levels of CK-MB and LDH, increased myocardial expressions of IL-18 and IL-1β, obvious myocardial damage, increased expression levels of NLRP3, caspase-1, GSDMD and GSDMD-N, and reduced expressions of EGFR, p-AKT and p-GSK3β proteins in the myocardial tissues. LJF treatment obviously improved myocardial function, decreased myocardial expressions of IL-18, IL-1β, NLRP3, caspase-1, GSDMD and GSDMD-N proteins, and increased the expressions EGFR, p-AKT and p-GSK3β proteins in DOX-treated mice. CONCLUSIONS: LJF extract alleviates DOX-induced myocardial injury in mice possibly by reducing myocardial inflammation and pyroptosis targeting EGFR, AKT and GSK3β to regulate the ErbB signaling pathway.
[Effect of AI-assisted compressed sensing acceleration on MRI radiomic feature extraction and staging model performance for nasopharyngeal carcinoma].
Li X, Xu G, Liu J … +1 more , Feng Y
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311094 · Full text
OBJECTIVES: To evaluate the effect of artificial intelligence-assisted compressed sensing (ACS) acceleration on MRI radiomic feature extraction and performance of diagnostic staging models for nasopharyngeal carcinoma (N...
OBJECTIVES: To evaluate the effect of artificial intelligence-assisted compressed sensing (ACS) acceleration on MRI radiomic feature extraction and performance of diagnostic staging models for nasopharyngeal carcinoma (NPC) in comparison with conventional parallel imaging (PI). METHODS: A total of 64 patients with newly diagnosed NPC underwent 3.0T MRI using axial T1-weighted (T1W), T2-weighted (T2W), and contrast-enhanced T1-weighted (CE-T1W) sequences. Both PI and ACS protocols were performed using identical imaging parameters. The total scan time for the 3 sequences in ACS group was 227 s, representing a 30% reduction from 312 s in the PI group. Eighteen first-order and 75 texture features were extracted using Pyradiomics. Intraclass correlation coefficients (ICCs) were calculated to assess the agreement between the two acceleration methods. After feature selection using the least absolute shrinkage and selection operator (LASSO), random forest regression models were constructed to distinguish early-stage (T1 and T2) from advanced-stage (T3 and T4) NPC. The diagnostic performance of the models was evaluated using the area under the receiver operating characteristic curve (AUC) and compared using the DeLong test. RESULTS: ACS-accelerated images demonstrated good radiomic reproducibility, with 86.0% (240/279) of features showing good agreement (ICC>0.75), with mean ICCs for T1W, T2W and CE-T1W sequences of 0.91±0.09, 0.89±0.13 and 0.88±0.11, respectively. The staging prediction models achieved similar AUCs for ACS and PI (0.89 0.90, =0.991). CONCLUSIONS: The MRI radiomic features extracted using ACS and PI techniques are highly consistent, and the ACS-based model shows comparable diagnostic performance to the PI-based model, but ACS significantly reduces the scan time and provides an efficient and reliable acceleration strategy for radiomics in NPC.
[Intubaiton with electromyographic endotracheal tube increases risks of postoperative sore throat following thyroidectomy under general anesthesia: a retrospective cohort study].
Chen L, Chen Y, Xie H … +3 more , Huang Y, Huang Y, Jin S
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311093 · Full text
OBJECTIVES: To investigate the effect of intubation with electromyographic (EMG) endotracheal tubes versus conventional wire-reinforced (CWR) tubes on the incidence of postoperative sore throat (POST) in patients undergo...
OBJECTIVES: To investigate the effect of intubation with electromyographic (EMG) endotracheal tubes versus conventional wire-reinforced (CWR) tubes on the incidence of postoperative sore throat (POST) in patients undergoing thyroidectomy under general anesthesia and identify the risk factors for POST. METHODS: We retrospectively collected the clinical data from a cohort of 245 patients undergoing elective thyroid surgery under general anesthesia at the Sixth Affiliated Hospital of Sun Yat-sen University between October, 2024 and March, 2025. Patients received intubation with either EMG endotracheal tubes (=100) or CWR tubes (=145) during the operation, and the incidences of POST and other postoperative complications were compared between the two groups. Propensity score matching (PSM) was applied to adjust for the baseline differences, and multivariate logistic regression analysis was used to identify independent risk factors for POST. RESULTS: Comparisons of the baseline data revealed significant differences between the two groups (<0.05). After PSM, 90 patients in EMG group and 75 in CWR group were included in the final analysis with matching baseline characteristics (>0.05). Post-matching analysis showed that the EMG group had a shorter operative time (=0.002) but a higher incidence of POST (=0.001). Multivariate logistic regression identified the use of EMG tubes (OR=17.50, 95% : 2.25-136.03, <0.01) as an independent risk factor for POST. CONCLUSIONS: Intubation with EMG endotracheal tubes can shorten the operative time and allow recurrent laryngeal nerve monitoring during thyroidectomy under general anesthesia, but their structural design may increase the risk of POST. Clinical decisions should be made to balance nerve protection and postoperative patient comfort by selecting appropriate tube types and optimizing intubation strategies to enhance perioperative outcomes.
[Design and validation of a multimodal model integrating text and imaging data for intelligent assessment of psychological stress in college students].
Xie H, Hu C, Liang G … +3 more , Han H, Huang M, Feng Q
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311092 · Full text
OBJECTIVES: We propose a multimodal model integrating social media text and image data for automated assessment of psychological stress in college students to support the development of intelligent mental health services...
OBJECTIVES: We propose a multimodal model integrating social media text and image data for automated assessment of psychological stress in college students to support the development of intelligent mental health services in higher education institutions. METHODS: Based on deep learning technology, we designed an evaluation framework comprising a text sentiment modeling module, an image sentiment modeling module, and a multimodal fusion prediction module. Text sentiment features were extracted using Bi-LSTM, and image semantic cues were extracted via U-Net. A feature concatenation strategy was used to enable cross-modal semantic collaboration to achieve automatic identification of 3 psychological stress levels: mild, moderate, and severe. We constructed a multimodal annotated dataset using social platform data from 1577 students across multiple universities in Guangdong Province. After data cleaning, 252 samples were randomly selected for model training and testing. RESULTS: In the 3-classification task, the model demonstrated outstanding performance on the test set, and achieved an accuracy of 92.86% and an F1 score of 0.9276, exhibiting excellent stability and consistency. Confusion matrix analysis further revealed the model's ability to effectively distinguish between different pressure levels. CONCLUSIONS: The multimodal psychological stress assessment model developed in this study effectively integrates unstructured social behavior data to enhance the scientific rigor and practical applicability of psychological state recognition, and thus provides support for developing intelligent psychological service systems.
[Impact of incorrect designation of working correlation structure matrix on sample size estimation in 2×2 cross design: a simulation study].
Zhang P, Xie Z, Zhuang Y
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311091 · Full text
OBJECTIVES: To investigate the impact of incorrect specification of the working correlation structure matrix on estimated sample size in a 2×2 crossover design based on the generalized estimating equation (GEE). METHODS:...
OBJECTIVES: To investigate the impact of incorrect specification of the working correlation structure matrix on estimated sample size in a 2×2 crossover design based on the generalized estimating equation (GEE). METHODS: Based on Monte Carlo simulation, the influence of incorrect specification of the work-related structure matrix on the sample size estimation under different conditions was evaluated after controlling the total sample size , the proportion of subjects assigned to AB sequence (s=1) , the correlation coefficient , and the placebo effect OR. Bias and mean square error (MSE) were used to assess the difference between the sample size estimates and the theoretical values. RESULTS: When the correctly specified working correlation structure matrix is independent, the sample size estimation effect of correctly specifying the working correlation structure matrix is better than that of incorrect specification. But when the correctly specified working correlation structure matrix is equal and the correlation coefficient is closer to 0, with other factors being smaller (≤50, θ≤0.5, OR=2 in this article), there is a situation where the bias of the sample size estimation value for the correctly specified working correlation structure matrix is greater than the bias for the incorrectly specified working correlation structure matrix. CONCLUSIONS: Under most conditions, incorrectly specifying the working correlation structure matrix can cause the estimated sample size to deviate significantly from the theoretical value, but under certain conditions, the impact of incorrectly specifying the working correlation structure matrix can be small on the estimated sample size.
[Niranthin ameliorates Crohn's disease-like enteritis in mice by inhibiting intestinal epithelial cell apoptosis and protecting intestinal barrier modulating p38/JNK signaling].
Tao L, Chen Y, Huang L … +4 more , Zheng W, Song X, Xiang P, Hu J
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311090 · Full text
OBJECTIVES: To investigate the therapeutic effect of the natural compound niranthin on Crohn's disease-like colitis in mice and explore the underlying molecular mechanisms. METHODS: In a mouse model of colitis induced by...
OBJECTIVES: To investigate the therapeutic effect of the natural compound niranthin on Crohn's disease-like colitis in mice and explore the underlying molecular mechanisms. METHODS: In a mouse model of colitis induced by 2,4,6-trinitro-benzenesulfonic acid (TNBS), the therapeutic effect of niranthin was evaluated by observing the changes in body weight, disease activity index (DAI), and colon length of the mice. The levels of inflammatory cytokines (IL-6, IL-1β, TNF-α, IL-17A and IL-10) in the intestinal mucosal tissue were detected using ELISA and quantitative real-time PCR (qRT-PCR). TUNEL staining and Western blotting were used to assess intestinal epithelial cell apoptosis and the expressions of Bcl-2 and Bax. The expression levels of tight junction proteins (ZO-1 and claudin-1) and the activation of the p38/JNK signaling pathway were investigated using Western blotting, and diprovocim intervention experiments were conducted to explore the molecular regulatory mechanism of niranthin. RESULTS: Niranthin treatment significantly increased body weight of TNBS-treated mice, lowered the DAI and histological inflammation scores, and increased colon length of the mice. The niranthin-treated mouse models showed obviously reduced protein and mRNA levels of IL-6, IL-1β, IL-17A, and TNF-α and upregulated expression of IL-10 in the colon tissue. TUNEL staining and Western blotting demonstrated that niranthin significantly inhibited intestinal epithelial cell apoptosis and activated the anti-apoptotic pathway in the mouse models. Niranthin treatment obviously upregulated the expression levels of ZO-1 and claudin-1 and downregulated the phosphorylation levels of p38 and JNK in the colon tissues of the mice. Diprovocim intervention obviously attenuated the inactivation of the p38/JNK signaling pathway induced by niranthin in the mouse models. CONCLUSIONS: Niranthin ameliorates TNBS-induced Crohn's disease-like colitis in mice by inhibiting intestinal epithelial cell apoptosis and protecting the integrity of the intestinal barrier regulating the activation of the p38/JNK signaling pathway.
[Silencing DDX17 inhibits proliferation and migration of pulmonary arterial smooth muscle cells by decreasing mTORC1 activity].
Deng X, Wang J, Xiong M … +4 more , Wang T, Yang Y, Li D, Sun X
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311089 · Full text
OBJECTIVES: To investigate the mechanism of DDX17 for regulating proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) during the development of pulmonary hypertension (PH). METHODS: In murine PA...
OBJECTIVES: To investigate the mechanism of DDX17 for regulating proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) during the development of pulmonary hypertension (PH). METHODS: In murine PASMCs cultured under normoxic or hypoxic conditions, the effects of transfection with si-Ddx17 and insulin treatment, alone or in combination, on cell proliferation and migration were evaluated using Ki-67 immunofluorescence staining, scratch assay and Transwell assay. Western Blotting was performed to detect the changes in protein expression levels of DDX17, 4EBP1, S6, p-4EBP1, and p-S6. In a mouse model of PH induced by intraperitoneal injection of monocrotaline (MCT), the changes in pulmonary vasculature were examined using HE staining following tail vein injection of AD-Ddx17i. RESULTS: The PASMCs in hypoxic culture exhibited significantly enhanced cell proliferation and migration and protein expressions of p-4EBP1 and p-S6, and these changes were obviously reversed by transfection with si-Ddx17. Treatment with insulin significantly attenuated the effect of si-Ddx17 against hypoxic exposure-induced changes in PASMCs. In the mouse model of MCT-induced PH, transfection with AD-Ddx17i obviously alleviated pulmonary vascular stenosis and intimal hyperplasia. CONCLUSIONS: The expression of DDX17 is elevated in hypoxia-induced PASMCs and PH mice, and silencing DDX17 significantly inhibits PASMC proliferation and migration and pulmonary vascular remodeling in PH mice by reducing mTORC1 activity.
[High-throughput circular RNA sequencing reveals tumor-specific high expression of hsa_circ_0001900 in Wilms tumor in association with poor prognosis].
Gao Z, Lin J, Hong P … +7 more , Hu Z, Cui K, Wang Y, Dong J, Shi Q, Tian X, Wei G
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311088 · Full text
OBJECTIVES: To explore the expression profile of circular RNAs (circRNAs) and their potential roles in prognosis and progression of Wilms' tumor (WT). METHODS: Four pairs of WT and adjacent tissues were collected for hig...
OBJECTIVES: To explore the expression profile of circular RNAs (circRNAs) and their potential roles in prognosis and progression of Wilms' tumor (WT). METHODS: Four pairs of WT and adjacent tissues were collected for high-throughput circRNA sequencing to identify the differentially expressed circular RNAs. RT-qPCR was used to verify the expression levels of the top 6 candidate circRNAs in the clinical samples. hsa_circ_0001900 was selected for analysis of its correlation with clinicopathological features and prognosis in 34 patients with WT. Sanger sequencing and RNase R digestion experiments were used to verify the cycling site and structural stability of hsa_circ_0001900 molecule. RESULTS: A total of 23 978 circular RNA molecules were identified in WT tissues by high-throughput circular RNA sequencing, and among them 614 were differentially expressed in WT. hsa_circ_0001900 showed the highest expression level among the differentially expressed circRNAs, which was consistent with the findings in clinical tumor samples and the sequencing results. Correlation analysis showed that hsa_circ_0001900 expression level was positively correlated with WT volume, and the children with high hsa_circ_0001900 expression had a lowered recurrence-free survival rate. The results of Sanger sequencing verified the circular splice site sequence of the molecule, and Rnase R digestion assay confirmed its stable covalent structure. CONCLUSIONS: This study presents a comprehensive expression profile of circular RNAs in WT, and the expression level of hsa_circ_0001900 is related to the size of WT and the patients' prognosis, suggesting its possible role as a key driving gene in WT progression.
[Hypaphorine alleviates Crohn's disease-like colitis in mice by inhibiting intestinal epithelial inflammatory response and protecting intestinal barrier function].
Huang Q, Yang J, Jiang X … +8 more , Zhang W, Wang Y, Zuo L, Wang L, Wang Y, Zhang X, Song X, Hu J
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311087 · Full text
OBJECTIVES: To investigate the effect of hypaphorine (HYP) on Crohn's disease (CD)‑like colitis in mice and its molecular mechanism. METHODS: Thirty male C57BL/6J mice were equally randomized into WT, TNBS, and HYP group...
OBJECTIVES: To investigate the effect of hypaphorine (HYP) on Crohn's disease (CD)‑like colitis in mice and its molecular mechanism. METHODS: Thirty male C57BL/6J mice were equally randomized into WT, TNBS, and HYP groups, and in the latter two groups, mouse models of CD-like colitis were established using TNBS with daily gavage of 15 mg/kg HYP or an equivalent volume of saline. The treatment efficacy was evaluated by assessing the disease activity index (DAI), body weight changes, colon length and histopathology. The effect of HYP was also tested in a LPS-stimulated Caco-2 cell model mimicking intestinal inflammation by evaluating inflammatory responses and barrier function of the cells using qRT-PCR and immunofluorescence staining. GO and KEGG analyses were conducted to explore the therapeutic mechanism of HYP, which was validated in both the cell and mouse models using Western blotting. RESULTS: In the mouse models of CD-like colitis, HYP intervention obviously alleviated colitis as shown by significantly reduced body weight loss, colon shortening, DAI and inflammation scores, and expressions of pro-inflammatory factors in the colon tissues. HYP treatment also significantly increased the TEER values, reduced bacterial translocation to the mesenteric lymph nodes, liver, and spleen, lowered serum levels of I-FABP and FITC-dextran, increased the number of colonic tissue cup cells, and upregulated colonic expressions of MUC2 and tight junction proteins (claudin-1 and ZO-1) in the mouse models. In LPS-stimulated Caco-2 cells, HYP treatment significantly inhibited the expressions of pro-inflammatory factors and increased the expressions of tight junction proteins. Western blotting showed that HYP downregulated the expressions of the key proteins in the TLR4/MyD88 signaling pathway in both the and models. CONCLUSIONS: HYP alleviates CD-like colitis in mice possibly by suppressing intestinal epithelial inflammation and improving gut barrier function.
[Construction of cardiac organoids derived from human induced pluripotent stem cells for cardiac disease modeling and drug evaluation].
Gong X, Fan Y, Luo K … +2 more , Yan Y, Li Z
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311086 · Full text
METHODS: Cardiac organoids derived from the self-assembled human induced pluripotent stem cells were constructed by regulating the Wnt signaling pathway. Flow cytometry was used to detect the proportion of cardiomyocytes...
METHODS: Cardiac organoids derived from the self-assembled human induced pluripotent stem cells were constructed by regulating the Wnt signaling pathway. Flow cytometry was used to detect the proportion of cardiomyocytes in the cardiac organoids, and RT-qPCR was employed to detect the mRNA expressions. Immunofluorescence staining was used to detect the protein expressions of TNNT2, CD31, and vimentin. The beating amplitude of the cardiac organoids was determined with calcium transient. myocardial injury models and ischemia-reperfusion models were established and the cell injuries were examined using Masson staining. TUNEL staining and calcium transient detection were used to evaluate the adverse effects of doxorubicin and trastuzumab in the cardiac organoids. RESULTS: The cardiac organoids began to beat on the 8th day of culture and consisted of 32.4% cardiomyocytes with high expressions of the myocardial markers TNNT2, NKX2.5, RYR2 and KCNJ2. No significant differences in morphological size, beating frequency, proportion of cardiomyocytes, or myocardial contractility were observed in the cardiac organoids differentiated from different batches. These cardiac organoids could be maintained in culture conditions for at least 50 days. Captopril treatment could obviously alleviate liquid nitrogen-induced myocardial injury in the cardiac organoids. Hypoxia/reoxygenation induced ischemia-reperfusion injury and promoted myocardial fibrosis and apoptosis in the cardiac organoids. Treatment with doxorubicin for 24 h resulted in significantly increased cell death and reduced beating frequency and cell viability in the cardiac organoids in a dose-dependent manner. Trastuzumab significantly impaired the contractile and calcium handling abilities of the cardiac organoids. CONCLUSIONS: Cardiac organoids derived from human induced pluripotent stem cells have been successfully constructed and can be used for cardiac disease modeling and drug evaluation.
[ Recipe improves high copper load-induced cognitive dysfunction in rats by regulating mitophagy].
Wang Y, Fang X, Chen Z … +4 more , Ruan B, Han X, Tang Y, Zhu L
Nan Fang Yi Ke Da Xue Xue Bao · 2025 Nov · PMID 41311085 · Full text
OBJECTIVES: To explore the mechanisms of Recipe (QLJR) for improving cognitive dysfunction in rats with high copper load. METHODS: Seventy-five male SD rats were randomized into normal control group, model group, QLJR g...
OBJECTIVES: To explore the mechanisms of Recipe (QLJR) for improving cognitive dysfunction in rats with high copper load. METHODS: Seventy-five male SD rats were randomized into normal control group, model group, QLJR group, penicillamine (PCA) group, and QLJR+ PCA group. Except for those in the control group, all the rats were fed a high-copper diet for 12 weeks. The effects of the treatments on cognitive function of the rats were assessed using the Barnes maze and passive avoidance tests. Hippocampal expressions of NIX, FUNDC1 and LC3 of the rats were detected using Western blotting and immunofluorescence staining, and changes in mitochondrial morphology were observed with transmission electron microscopy. RESULTS: Behavioral tests showed prolonged target hole latency, shortened latency to enter the dark chamber, and increased error counts of the rats in the model group, which were significantly improved in QLJR+PCA group; the error counts were significantly lower in QLJR+PCA group than in either QLJR or PCA group. Among all the groups, the hippocampal expressions of NIX and FUNDC1 were the lowest and LC3 I/II expression the highest in the model group; NIX and FUNDC1 expressions were significantly higher and LC3 I expression was lower in QLJR+PCA group than in QLJR group and PCA group. Immunofluorescence staining revealed weakened NIX and FUNDC1 expressions and enhanced LC3 expression in the hippocampus of the rats in the model group as compared with those in the normal control and QLJR+PCA groups, but their expressions did not differ significantly between QLJR and PCA groups. The rats in the model group showed obvious structural disarray of the mitochondria, which were improved in all the treatment groups. CONCLUSIONS: QLJR improves cognitive dysfunction in rats with high copper load possibly by regulating mitophagy.
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