Taibi M, Elbouzidi A, Bentouhami NE
… +12 more, Haddou M, Baraich A, Hammouti Y, Belbachir Y, Bellaouchi R, Mothana RA, Hawwal MF, Asehraou A, Karboune S, Addi M, Guerrouj BE, Chaabane K
Skin Res Technol
· 2025 Jun · PMID 40515377
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BACKGROUND: The growing demand on natural ingredients in cosmetics has immensely contributed to a renewed interest in cosmetic industry in plant derivatives, especially essential oils. The aim of this study is to examine...BACKGROUND: The growing demand on natural ingredients in cosmetics has immensely contributed to a renewed interest in cosmetic industry in plant derivatives, especially essential oils. The aim of this study is to examine the dermatoprotective and antifungal properties of Clinopodium nepeta (CNEO) and Thymus vulgaris (TVEO) essential oils. MATERIALS AND METHODS: Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the chemical composition of the essential oils. Anti-elastase and anti-tyrosinase activities were evaluated using standard enzymatic inhibition assays, and IC values were calculated. Photoprotective properties were determined using in vitro sun protection factor (SPF) calculations. Antifungal and antibacterial activities were assessed using the disc diffusion method and minimum inhibitory concentration (MIC) determination against Candida albicans, Candida glabrata, Micrococcus luteus, and Staphylococcus aureus. RESULTS: GC-MS analysis revealed the presence of 13 compounds in CNEO, mainly oxygenated monoterpenes (91.9%) with pulegone (42.3%) as the main component, and 25 compounds in TVEO, with α-terpineol (19.8%) and carvacrol (13.5%) as the dominant compounds. CNEO showed superior anti-elastase activity (IC = 13.55 ± 0.81 µg/mL) compared with TVEO (IC = 28.40 ± 2.64 µg/mL). Both oils demonstrated significant anti-tyrosinase effects, with CNEO showing greater efficacy in inhibiting monophenolase (IC = 36.71 ± 4.09 µg/mL) and diphenolase (IC = 22.77 ± 0.97 µg/mL) than TVEO. SPF calculations revealed notable photoprotective properties for both oils, with CNEO (SPF = 6.472) slightly outperforming TVEO (SPF = 5.640). Antifungal tests against C. albicans and C. glabrata, and antibacterial tests against M. luteus and S. aureus showed that both oils possess strong antifungal and antibacterial activities, with CNEO demonstrating superior efficacy (MIC = 0.50 ± 0.00% v/v for both Candida strains) compared with TVEO (MIC = 0.011 ± 0.00% v/v for both Candida strains). CONCLUSION: This study provides the first comprehensive assessment of the dermatoprotective, antifungal, and antibacterial activities of CNEO and confirms the potential of TVEO in cosmetic, antifungal, and antibacterial applications. The results suggest that these essential oils could serve as promising natural ingredients in dermatoprotective, antifungal, and antibacterial formulations.
Skin Res Technol
· 2025 Jun · PMID 40504120
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BACKGROUND: Adipose-derived stem cells (ADSCs) exhibit good anti-photoaging activity, yet inadequate homing numbers and low cell survival rates remain urgent challenges to overcome, and the underlying mechanisms are stil...BACKGROUND: Adipose-derived stem cells (ADSCs) exhibit good anti-photoaging activity, yet inadequate homing numbers and low cell survival rates remain urgent challenges to overcome, and the underlying mechanisms are still unclear. METHODS: Methylcellulose (MC)/hyaluronic acid (HA)/Collagen I hydrogel (Gel) loaded with ADSCs (MHAC/ADSCs Gel) was prepared using the thermal dispersion method. Scanning electron microscopy was used to observe its microstructure, while swelling and degradation rates were determined by the pycnometer method. Subsequently, a photoaging HaCat cell model was established to evaluate the anti-photoaging potential of MHAC/ADSCs using the CCK-8 assay and Transwell experiment. Furthermore, qRT-PCR, WB, TargetScan bioinformatics analysis, and dual-luciferase reporter assays were conducted to investigate the underlying mechanisms of MHAC/ADSCs. Finally, a photoaging mouse model was established to assess the anti-photoaging activity of MHAC/ADSCs using H&E staining, Masson staining, immunohistochemistry, qRT-PCR, and WB. RESULTS: MHAC/ADSCs exhibited a 3D porous structure, remaining in a sol state at 20C and forming a gel at 37C. Additionally, it demonstrated excellent swelling properties and degradability. The results also revealed that MHAC/ADSCs showed significant anti-photoaging activity, promoting the migration of HaCat cells, increasing the expression of miR-18a and Collagen I, and inhibiting the expression of HIF-1α. In animal experiments, MHAC/ADSCs reversed epidermal thickening and collagen loss in mice and reduced the expression level of CD31. CONCLUSION: MHAC/ADSCs exhibit anti-photoaging activity by inhibiting the expression of HIF-1α through the upregulation of miR-18a, providing a promising approach for clinical treatment of photoaging.
Ha YJ, Tak KH, Lee JL
… +5 more, Kim CW, Ah YC, Kim SS, Moon IJ, Yoon YS
Skin Res Technol
· 2025 Jun · PMID 40484710
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BACKGROUND: Atopic dermatitis (AD) is a chronic inflammatory dermatological disorder characterized by skin barrier dysfunction, dry skin, pruritus, and aberrant immune responses to external stimuli. Although polynucleoti...BACKGROUND: Atopic dermatitis (AD) is a chronic inflammatory dermatological disorder characterized by skin barrier dysfunction, dry skin, pruritus, and aberrant immune responses to external stimuli. Although polynucleotides (PNs) have anti-inflammatory properties, their effect on AD remains unexplored. MATERIALS AND METHODS: This study investigated the effects of PNs on a 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model. The effects were evaluated by the dermatitis severity score (DSS), the spleen index, the serum immunoglobulin E (IgE) concentration, trans-epidermal water loss (TEWL), histological findings, and the expression levels of cytokine mRNA and filaggrin protein in skin tissue. RESULTS: Topical application of PNs significantly reduced the DSS, the spleen index, the serum IgE concentration, and TEWL compared with the control. Additionally, histopathological analysis showed that PNs reduced epidermal and dermal thickness, the mast cell count, collagen deposition, and eosinophil infiltration in the dermis. Moreover, PNs significantly downregulated the expression of key inflammatory cytokines, including interleukin (IL)-4, IL-5, IL-13, IL-25, IL-33, and thymic stromal lymphopoietin (TSLP), in affected skin tissue. Immunohistochemical (IHC) staining and Western blot revealed that PNs inhibited DNCB-induced suppression of filaggrin. A combination of hyaluronic acid (HA) and PNs showed enhanced efficacy compared with PNs alone, particularly for reducing the serum IgE concentration and TEWL and increasing filaggrin expression. CONCLUSION: These results suggest that PNs are potential candidates to treat AD because they possess anti-inflammatory properties and improve skin barrier function.
Skin Res Technol
· 2025 Jun · PMID 40484704
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BACKGROUND: Supramolecular technology has been widely applied in cosmetics due to its ability to enhance the stability of active substances and reduce their irritation potential. The synergistic interaction between polys...BACKGROUND: Supramolecular technology has been widely applied in cosmetics due to its ability to enhance the stability of active substances and reduce their irritation potential. The synergistic interaction between polysaccharides can produce new products with characteristics and rheological properties that make them suitable for various applications. OBJECTIVE: The supramolecularly assembly of Dendrobium officinale polysaccharide (DOP) and hyaluronic acid (HA) was produced, and its moisturizing effect was verified to provide a basis for the application of supramolecular technology in cosmetic functional raw materials. METHODS: The supramolecular structure of polysaccharide assembly samples was verified through scanning electron microscopic observation. Moisture absorption and the ability of samples to resist cell drying damage before and after supramolecular assembly were evaluated by moisture absorption experiments and a cell drying damage model, respectively. Furthermore, this study validated the moisturizing effect of the supramolecular compositions during their application in cosmetics through tests of skin moisture content in extremely dry environments (during the winter season of Beijing, China), skin roughness and scale index, and trans epidermal water loss. RESULTS: The supramolecular composition appeared as a large sheet-like structure under a scanning electron microscope. Further, the supramolecular composition had significantly improved moisture absorption performance and could effectively resist cell drying damage. In an extremely dry environment, the composition showed excellent moisturizing performance and effectively improved skin scale and roughness. CONCLUSIONS: The supramolecular assembly of DOP and HA has a synergistic moisturizing effect; thus, it can be expected to have a good moisturizing effect when applied to cosmetics.
Michelini S, Greco ME, Vespasiani G
… +5 more, Trovato F, Chello C, Musolff N, Cantisani C, Pellacani G
Skin Res Technol
· 2025 · PMID 40411804
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BACKGROUND: Skin aging represents a para-physiological process related to metabolic imbalances, inflammation, proliferative responses, and oxidative stress. Ending the cycle of inflammation, and oxidative stress represen...BACKGROUND: Skin aging represents a para-physiological process related to metabolic imbalances, inflammation, proliferative responses, and oxidative stress. Ending the cycle of inflammation, and oxidative stress represents a way to slow the effects of aging. STUDY OBJECTIVE: The aim of our study was to evaluate the benefits of supplements with an herbal mixture based on Venerinase and B-group vitamins, Zinc, and Magnesium in preventing/reducing photoaging by means of objective quantitative analysis of visual skin features obtained by VISIA 2D and stroma and vascular characteristics measured by Optical Coherence Tomography (OCT). METHODS: A prospective clinical study was conducted in 40 participants. They were randomized 1:1 to receive the supplement (Giovina) or placebo. Assessments were conducted at Baseline (T0), after 2 months (T1), and after 4 months (T2). The primary endpoint was to obtain a significant improvement in facial photo/chrono-aging in the treatment arm, as assessed through the evaluation of the Griffith scale. Secondary endpoints were to assess significant changes in skin aging objective features (VISIA) and Optical Coherence Tomography (OCT) parameters. RESULTS: The treated group showed a significant reduction in the mean Griffith scale score from 4.5 at baseline to 3.76 at T2 (4 months) (p = 0.001). A significant decrease in red areas and a borderline significant reduction in the number of wrinkles have been shown by skin aging objective feature calculation on VISIA 2D photographs. OCT analysis showed a borderline significant reduction in collagen density. CONCLUSIONS: The decrease in vascular pattern and collagen density suggests an anti-inflammatory effect with a potential stromal remodeling in the treated group. This seems to be correlated to the reduction in wrinkles and facial redness.
Ward J, Verucchi E, Swaile D
… +3 more, Parker K, Worsley PR, Filingeri D
Skin Res Technol
· 2025 · PMID 40366638
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BACKGROUND: Experiencing wetness on the skin because of sweating or contact with fluids can induce thermal discomfort. Millions of people apply antiperspirant deodorant products to the underarm to minimise this negative...BACKGROUND: Experiencing wetness on the skin because of sweating or contact with fluids can induce thermal discomfort. Millions of people apply antiperspirant deodorant products to the underarm to minimise this negative experience. However, the mechanisms underpinning wetness perception at the underarm and the influence of underlying stratum corneum hydration remain under investigation. We aimed to evaluate the role of stimulus temperature and skin hydration levels on wetness perception at the underarm in young participants. MATERIALS AND METHODS: Ten healthy participants (5 M/5 F; 29 ± 7 years) underwent a quantitative sensory test during which they reported the perceived magnitude of wetness perception from a short-duration static application of a cold-wet (i.e., 5°C below local skin temperature), neutral-wet (i.e., equal to local skin temperature) and warm-wet (i.e., 5°C above local skin temperature) stimuli. Wetness perception was assessed on a 100-mm visual analogue scale (0 = dry; 100 = completely wet), with a repeated measures design exploring the effects of overhydration (+21 %) and dehydration (-40 %) of the underarm's skin. RESULTS: Our results indicated a higher wetness perception (p = 0.012) during the cold-wet (40 mm, 95 % CI: 25, 56) than during the warm-wet (25 mm, 95 % CI: 12, 39), and neutral-wet stimuli (24 mm, 95 % CI: 7, 40). Furthermore, overhydration of the underarm' stratum corneum can lead to an increase in wetness perceptions upon contact with cold-wet stimuli only (mean increase: 20 mm, 95 % CI: 3, 36; p = 0.024; corresponding to 20 % increase). CONCLUSION: Our findings provide novel fundamental insights into the underarm's perceptual responses to wetness, which could inform understanding of the determinants of wet feel associated with periods of sweating and the application of antiperspirant products.
Du M, Wang Y, Wang J
… +3 more, Xie Y, Xu Y, Yang D
Skin Res Technol
· 2025 · PMID 40321074
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OBJECTIVE: The aim of this paper is to review the current status and recent progress of the application of novel non-invasive testing technologies in the diagnosis, treatment, and care of scalp hair diseases in recent ye...OBJECTIVE: The aim of this paper is to review the current status and recent progress of the application of novel non-invasive testing technologies in the diagnosis, treatment, and care of scalp hair diseases in recent years, and to discuss their contribution to the improvement of scalp hair health management. METHODS: Through a literature review, the principles and current status of application of technologies such as trichoscopy, microbiological testing, scalp skin physiological function testing, hair physiological index testing, and hair product patch testing, and their specific roles in the management of scalp hair diseases were systematically sorted out. RESULTS: Trichoscopic intelligent analysis technology can quantitatively assess the hair growth status and provide an objective assessment of the efficacy of hair diseases; microbiological testing provides a laboratory reference for the rapid diagnosis of scalp diseases and clinical use of medication; scalp skin physiological function testing and hair physiological index testing are essential for a comprehensive assessment of the scalp environment and hair health. The patch test can effectively detect allergenic substances, which can help to safely select topical drugs and hair care products, avoid medical risks, and facilitate safety evaluation. CONCLUSION: Novel non-invasive testing techniques provide powerful technical support for accurate diagnosis, personalized treatment, and safe care of scalp hair diseases, and show a broad application prospect. Future research should focus on improving the accuracy and detection efficiency of these techniques to meet the growing demand for scalp hair health management and to promote the development of this field in a more scientific and refined direction.
Skin Res Technol
· 2025 · PMID 40321073
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BACKGROUND: Wound healing is a complex process with significant economic implications. Hyaluronic acid (HA), valued for its adaptability and biocompatibility, shows the potential to improve multiple facets of wound heali...BACKGROUND: Wound healing is a complex process with significant economic implications. Hyaluronic acid (HA), valued for its adaptability and biocompatibility, shows the potential to improve multiple facets of wound healing. Despite the expanding literature on the use of HA in wound care, a comprehensive analysis of its scholarly evolution is lacking. This study employs a bibliometric approach to objectively evaluate trends in scholarly publications regarding HA's role in promoting wound healing. METHODS: We searched in the Web of Science Core Collection (WoSCC) for articles published from January 1, 2000 to March 31, 2024. We extracted relevant information about using HA to promote wound healing following a thorough screening process. Subsequently, a comprehensive analysis was undertaken on a total of 1886 publications. The analysis utilized GraphPad Prism 9, CiteSpace6.1.6, VOSviewer1.6.19, the Online Analysis Platform of Literature Metrology (http://bibliometric.com/), GeneMANIA (https://genemania.org/), and Metascape (https://metascape.org/gp/index.html#/main/step1). RESULTS: We retrieved 2424 publications on hyaluronic acid (HA) and wound healing from the Web of Science Core Collection, covering the period from January 2000 to March 2024, and selected 1886 for analysis. The results show a significant increase in publications since 2016, reflecting a growing focus on this field. Currently, China's publication volume has surpassed the United States since 2017, indicating a significant rise in China's influence in this area. Using CiteSpace software for co-citation analysis, we identified eight main research clusters, including promoted wound healing, injured tissue, and advanced multi-targeted composite biomaterial. Key research areas involve the role and mechanisms of hyaluronic acid in tissue repair, particularly its applications in growth factor production and regenerative therapy. Analyzing keyword co-occurrence and burst data with VOSviewer, we identified research hotspots focused on biomaterials, such as nanoparticles and hydrogels, and their antibacterial properties. The keyword "CD44" showed a long burst period, while "antibacterial" had the highest burst intensity in 2022. We identified the top 21 genes extensively studied in hyaluronic acid and wound healing, including CD44, VEGF, and TGF-β. These genes are mainly involved in regulating cell migration, adhesion, proliferation, and cytokine activity. GO enrichment and KEGG pathway analyses indicate that these genes are associated with key signaling pathways, such as MAPK and EGFR, revealing the primary mechanisms hyaluronic acid promotes wound healing. CONCLUSION: This pioneering study provides the first comprehensive bibliometric analysis of HA in wound healing. Covering the period from January 1, 2000 to March 31, 2024, it reveals a significant expansion in annual scholarly production. Current research emphasizes the development of HA-based biomaterials for enhancing wound healing.
Sasaoka S, Gabe Y, Uchiyama M
… +4 more, Stepp A, Imai A, Hachiya A, Kiyomine A
Skin Res Technol
· 2025 · PMID 40321063
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BACKGROUND: Dry skin is a universal skin concern that is often accompanied by itching, but moisturizers have not completely solved this issue. We found that the combination of a moisturizer (lotion) and an ultra-thin fil...BACKGROUND: Dry skin is a universal skin concern that is often accompanied by itching, but moisturizers have not completely solved this issue. We found that the combination of a moisturizer (lotion) and an ultra-thin film of fine fiber (FF) that we developed improved dry skin early, but the biological mechanism within the skin remained unclear. MATERIALS AND METHODS: Eight (Study 1) and seven (Study 2) subjects used a lotion with and without the FF film on their lower legs. In addition to measurement of visual skin dryness scores and conductance, proteomic analysis of stratum corneum (SC) samples at baseline and 14 days after treatment in Study 1 and immunohistochemical staining of skin biopsy samples 3 or 5 days after treatment in Study 2 were performed. RESULTS: Skin dryness scores and conductance improved more when the lotion was used with the FF film than with the lotion alone. The protein expression level of suprabasin, which is related to epidermal differentiation and barrier function, increased within a few days near the granular layer, as well as in the SC after 14 days. Proteomic analysis using SC samples showed that the FF film treatment with lotion decreased annexin A2, a dry skin marker, whereas it increased retroviral-like aspartic protease 1, which is related to skin water content, more than lotion alone. CONCLUSION: The present findings suggest that lotion with the FF film initiates restoration of epidermal homeostasis, leading to early improvement of dry skin.
Skin Res Technol
· 2025 · PMID 40321042
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BACKGROUND: Investigating the skin penetration behavior of pharmaceuticals and cosmetics is crucial. Due to the Raman-active properties of many active ingredients, Confocal Raman microspectroscopy (CRM) emerges as a prom...BACKGROUND: Investigating the skin penetration behavior of pharmaceuticals and cosmetics is crucial. Due to the Raman-active properties of many active ingredients, Confocal Raman microspectroscopy (CRM) emerges as a promising, non-invasive technique for penetration studies. AIM: To evaluate the in vivo penetration behavior of yeast rice ferment filtrate (RFF) into the skin using CRM. METHODS: Raman spectra of RFF were acquired at 0.5, 1, 2, and 4 h post-application using CRM. The penetration of RFF at various skin depths (10-40 µm) and different time points was analyzed. Multivariate analysis was applied to generate Raman spectral maps that can show RFF depth penetration and spatial distribution across different skin layers. RESULTS: RFF surpassed the stratum corneum barrier within 30 min and penetrated into the dermis layer after 4 h of application, indicating rapid permeation kinetics. Quantitative analysis, based on Raman spectra, showed increasing RFF penetration over time, reaching 1.02%, 3.17%, 5.29%, and 8.75% after 0.5, 1, 2, and 4 h, respectively. The heat map visualized depth penetration and spatial distribution, indicating a significant increase in penetration with extended application durations. CONCLUSION: CRM proves to be a powerful tool for studying the in vivo penetration of RFF into the skin, offering valuable insights into the complex dynamics underlying its permeation behavior.