Zhang Y, Liu X, Zhang Z
… +6 more, Sun W, Yue W, Liu L, Zhu Y, Zhang X, Wang H
J Cardiovasc Transl Res
· 2025 Oct · PMID 40691389
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Hypertrophic cardiomyopathy (HCM) is one of the most prevalent hereditary cardiovascular diseases. Eicosanoids are known to play a significant role in cardiovascular diseases and serve as biomarkers. In this study, plasm...Hypertrophic cardiomyopathy (HCM) is one of the most prevalent hereditary cardiovascular diseases. Eicosanoids are known to play a significant role in cardiovascular diseases and serve as biomarkers. In this study, plasma eicosanoids were profiled by LC-MS in a cohort of 78 healthy individuals and 73 patients diagnosed with HCM. Our findings reveal HCM patients exhibit downregulation of various eicosanoids, including AA, 5,6-DHET, 12-HETE, LXA4, EPA, etc. Notably, the combined predictive model incorporating 12-HETE and EPA demonstrates significant diagnostic value for HCM. Additionally, ten closely related metabolites showed significant positive correlations within the metabolic network graph. Eicosanoids such as 17,18-EEQ, LXA4, and 13-oxo-ODE exhibit significant negative correlations with plasma concentrations of hs-CRP and NT-proBNP in patients. Alterations in eicosanoid metabolism may be implicated in the pathophysiological processes underlying HCM.
Bhave S, Swain L, Reyelt L
… +9 more, Qiao X, Aryaputra T, Everett K, John K, Berry I, Stolyaranov A, Mahmoudi E, Chin M, Kapur NK
J Cardiovasc Transl Res
· 2025 Oct · PMID 40691388
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Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) provides cardiopulmonary support during the critical care. Despite its exponential use over last few decades, clinical studies suggest that it does not improve...Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) provides cardiopulmonary support during the critical care. Despite its exponential use over last few decades, clinical studies suggest that it does not improve infarct size and mortality in patients suffering from cardiogenic shock after the acute myocardial infarction (AMI). The effect of hyperoxygenated blood under high pressure on the poor outcomes associated with VA-ECMO have not been tested. In this study we tested the hypothesis whether normoxemic oxygenation of blood during provides protection against VA-ECMO associated cardiac damage using a swine model of AMI. Our results indicate that normoxemic VA-ECMO (PaO2 < 200 mmHg), reduces infarct size, activates pro-survival RISK pathway and furthermore preserves mitochondrial structure and function as compared to hyperoxygenated VA-ECMO (PaO2 > 200 mmHg) in ischemia-reperfusion injury. In absence of conclusive clinical trials testing the effect of VA-ECMO blood oxygenation on the patients' outcomes, our findings provide new mechanistic insight into the myocardial effects of VA-ECMO.
Amirsardari Z, Moghadam EA, Mohebbi A
… +5 more, Fanos V, Ayati A, Portman MA, Tahghighi F, Ziaee V
J Cardiovasc Transl Res
· 2025 Aug · PMID 40681906
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We investigated the metabolomic profile of Kawasaki disease(KD) and its association with the development of coronary artery aneurysms (CAA) and resistance to intravenous immunoglobulin(IVIG) therapy. Metabolomic profilin...We investigated the metabolomic profile of Kawasaki disease(KD) and its association with the development of coronary artery aneurysms (CAA) and resistance to intravenous immunoglobulin(IVIG) therapy. Metabolomic profiling was performed on 26 patients with KD. A total of 44 metabolites-including 12 amino acids, L-carnitine, and 31 acylcarnitines-were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after IVIG administration. Methylglutarylcarnitine, a medium-chain acylcarnitine, was identified as a differentially expressed metabolite in KD patients with CAA. Additionally, C2, C3, C14, C16, C16OH, C18:2, and C18:2OH were differentially expressed between IVIG-resistant and IVIG-responsive patients. Pathway analysis using the KEGG database revealed that arginine biosynthesis and alanine, aspartate, and glutamate metabolism were among the most affected pathways in KD patients with CAA. This study demonstrated several differentially expressed metabolites in KD patients with CAA and IVIG resistance. These findings provide new insights into the metabolic pathways underlying KD complications.
Foglieni C, Ravanelli D, Lombardi M
… +6 more, Spartano L, Pieri M, Ajello S, Landoni G, Scandroglio AM, Consolo F
J Cardiovasc Transl Res
· 2025 Oct · PMID 40643853
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We tested whether platelet microRNAs (miRs) expression can predict bleeding in patients supported with the HeartMate3 (HM3) left ventricular assist device (LVAD). The levels of expression of platelet miR-126, miR-233, mi...We tested whether platelet microRNAs (miRs) expression can predict bleeding in patients supported with the HeartMate3 (HM3) left ventricular assist device (LVAD). The levels of expression of platelet miR-126, miR-233, miR-151a, and miR-454 were prospectively measured in 12 consecutive patients pre-implant and during long-term follow-up and compared based on aspirin (ASA) or ASA-free therapy at discharge and bleeding events over the course of HM3 support. Median time of follow-up was 760 (574-844) days. Seven (58%) patients were discharged on ASA and 5 (42%) without ASA. Three (25%) patients experienced 10 bleeding episodes: one never received ASA, and two suffered from bleeding recurrency long after permanent ASA discontinuation. ASA had minimal influence on platelet miRs expression. The expression levels of platelet miR-454 were lower in bleeders vs. non-bleeders, both pre-implant and post-implant. This study suggests the existence of a patient-specific pro-hemorrhagic phenotype associated with a distinctive expression profile of platelet miR-454. Clinical Trial Registration: https://clinicaltrials.gov/ct2/show/NCT03255928 . ClinicalTrials.gov Identifier: NCT03255928.
J Cardiovasc Transl Res
· 2025 Aug · PMID 40637998
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Cardiovascular disease (CVD) is the leading cause of death in the diabetic population. There is a need for specific predictive markers to assess CVD risk. The present study explored the plasma proteome profile of treadmi...Cardiovascular disease (CVD) is the leading cause of death in the diabetic population. There is a need for specific predictive markers to assess CVD risk. The present study explored the plasma proteome profile of treadmill test (TMT) assessed diabetic stress test positive (DSTP) and diabetic stress test negative (DSTN) subjects by performing a SWATH-MS-based label-free quantitative mass spectrometry approach to identify differentially expressed proteins (DEPs). CVD-relevant DEPs were further validated using a targeted mass spectrometry approach (MRM-HR). It was observed that CO4B, PON1 and LUM exhibited considerable differential expression in both the MS approaches, and ROC analysis showed significant AUC (0.97, 0.79 and 0.77, respectively). Overall, the present study reports these proteins as potential alternative markers for TMT in assessing CVD risk. These markers can possibly overcome the limitations of TMT with further validation in the large cohort.
Powrózek T, Mazurek M, Skwarek-Dziekanowska A
… +4 more, Sobieszek G, Maffeo D, Frullanti E, Małecka-Massalska T
J Cardiovasc Transl Res
· 2025 Dec · PMID 40637997
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Cachexia, often seen in chronic heart failure (CHF), worsens patient outcomes and survival. Early detection is crucial, and circulating miRNAs offer potential as biomarkers linking heart function, inflammation, and cache...Cachexia, often seen in chronic heart failure (CHF), worsens patient outcomes and survival. Early detection is crucial, and circulating miRNAs offer potential as biomarkers linking heart function, inflammation, and cachexia. This study aimed to identify plasma miRNAs associated with cachexia in CHF and assess their diagnostic and prognostic value. Plasma samples from 150 newly diagnosed CHF patients were analyzed using next-generation sequencing (NGS) and validated by qRT-PCR. A signature of elevated miRNA-628 and reduced miRNA-6803 (↑miRNA-628+↓miRNA-6803) was associated with poor nutritional status, abnormal lab results, and higher cachexia risk. Combining this signature with inflammatory markers perfectly distinguished cachectic from non-cachectic patients (AUC=1.0). This profile increased cachexia risk 19-fold and was linked to significantly shorter survival (median 14 vs. 41 months). Thus, the identified miRNA signature offers strong predictive and diagnostic potential and could complement clinical assessments of CHF patients' nutritional status.
Jiang X, Lei Y, Yin Y
… +3 more, Ma F, Liu G, Zheng M
J Cardiovasc Transl Res
· 2025 Oct · PMID 40622446
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This study aimed to explore the association between serum ferritin and glutathione peroxidase 4 (Gpx4), markers of ferroptosis, with the severity of coronary artery stenosis and the occurrence of in-stent restenosis afte...This study aimed to explore the association between serum ferritin and glutathione peroxidase 4 (Gpx4), markers of ferroptosis, with the severity of coronary artery stenosis and the occurrence of in-stent restenosis after percutaneous coronary intervention (PCI) in coronary artery disease (CAD) patients. The study encompassed 396 eligible CAD patients who underwent PCI, followed up for one year. A control group of 100 individuals was selected, matching the baseline data of CAD patients. Compared to healthy controls (HC), CAD patients showed a significant decrease in serum Gpx4 levels and an increase in ferritin levels. Significant disparities were observed in low-density lipoprotein cholesterol, Gensini score, serum Gpx4, and ferritin between the restenosis and no-restenosis groups (p < 0.001). Serum levels of ferritin and Gpx4, markers associated with ferroptosis, correlate with the severity of coronary artery stenosis in CAD patients and the occurrence of in-stent restenosis after PCI.
J Cardiovasc Transl Res
· 2025 Oct · PMID 40622445
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Coronary artery disease (CAD) is increasingly recognized as a chronic inflammatory condition. Interleukin-27 (IL-27), a cytokine from the IL-12 and IL-6 families, plays a dual role in CAD pathogenesis. It exacerbates dis...Coronary artery disease (CAD) is increasingly recognized as a chronic inflammatory condition. Interleukin-27 (IL-27), a cytokine from the IL-12 and IL-6 families, plays a dual role in CAD pathogenesis. It exacerbates disease by interacting with IL-1β and activating the NLRP3 inflammasome, promoting inflammation and tissue damage. Conversely, IL-27 can delay disease progression by engaging STAT1/3 signalling, suppressing inflammation, and promoting tissue repair. Future research should focus on elucidating IL-27's specific biological functions, interactions with molecular targets, and clinical implications in CAD. This will enhance understanding of CAD and support the development of improved diagnostic and therapeutic strategies.
J Cardiovasc Transl Res
· 2025 Dec · PMID 40622444
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Diabetic cardiomyopathy (DCM), defined as cardiac impairment resulting from the development of diabetes mellitus, has emerged as a global epidemic. DCM primarily denotes myocardial metabolic impairment induced by elevate...Diabetic cardiomyopathy (DCM), defined as cardiac impairment resulting from the development of diabetes mellitus, has emerged as a global epidemic. DCM primarily denotes myocardial metabolic impairment induced by elevated glucose levels, with hyperglycemia serving as a separate risk factor for heart disease in a lack of hypertension and coronary atherosclerosis. Exosomes, a category of tiny extracellular vesicles essential for communication between cells, have attracted considerable interest for their prospective use in the investigation and management of DCM. Exosomes harbour bioactive chemicals that indicate specific physiological conditions, modulate bioenergetic metabolism, and facilitate tissue healing. This review elucidates the dynamic progression of exosome research in DCM through bioenergetic disruptions, encompassing different processes such as endothelial dysfunction, mitochondrial impairment, calcium signalling in the endoplasmic reticulum, cell death including apoptosis and ferroptosis, inflammation-related fibrosis, autophagy, and hypertrophy. It emphasizes the necessity for continued research to fully exploit the potential of exosomes in enhancing patient outcomes.
J Cardiovasc Transl Res
· 2025 Oct · PMID 40618008
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Myocardial markers and inflammatory factors have been identified as risk factors for the development of sepsis-induced cardiac dysfunction (SIC), the prognostic significance and clinical utility of utilizing a multi-inde...Myocardial markers and inflammatory factors have been identified as risk factors for the development of sepsis-induced cardiac dysfunction (SIC), the prognostic significance and clinical utility of utilizing a multi-index combined prognostic approach remain uncertain. Our objective was to establish a "risk score" utilizing clinical indexes to provide short-term prognostic insight for individuals with SIC. Utilizing Lasso regression and COX regression analysis, we identified a history of diabetes, CRP, and NT-proBNP on day 3 as independent risk factors for 28-day mortality in SIC patients. Specifically, the Cox regression analysis identified a hazard ratio of 24.74 (95% CI: 5.90-103.71) for the high-risk group. The prognostic thresholds for CRP and NT-proBNP were determined to be 83.3 mg/L and 1720.7 ng/L, respectively. In conclusion, a "risk score" predictive model incorporating diabetes history, CRP, and NT-proBNP was developed to assess 28-day mortality in patients with SIC.
Chen X, Xu Q, Ding W
… +5 more, Wang Y, Wang P, Zhao C, Ao X, Wang J
J Cardiovasc Transl Res
· 2025 Oct · PMID 40593298
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Myocardial infarction (MI) remains a leading cause of mortality, and although reperfusion therapy is essential for myocardial salvage, it often results in ischemia-reperfusion (I/R) injury, which contributes substantiall...Myocardial infarction (MI) remains a leading cause of mortality, and although reperfusion therapy is essential for myocardial salvage, it often results in ischemia-reperfusion (I/R) injury, which contributes substantially to cardiomyocyte necrosis. Although the mechanisms of cardiomyocyte necrosis remain unclear, we identified ACSS2 as a key regulator in myocardial I/R injury. ACSS2 was upregulated under oxidative stress and I/R conditions. Its knockdown reduced necrosis, while overexpression aggravated it. Mechanistically, nuclear translocation of ACSS2 enhanced H3K9 acetylation and activated necrosis-related genes. In vivo, ACSS2 silencing alleviated myocardial injury and improved cardiac function. These findings reveal that ACSS2 promotes necrosis via nuclear acetyl-CoA production and epigenetic regulation, offering a potential therapeutic target for I/R injury.
Simonetti F, Voll F, Alfonso F
… +16 more, Gräßer C, Janisch M, Joner M, Kessler T, Kuna C, Leistner DM, Lenz T, Presch A, Rheude T, Sager H, Schunkert H, Starnecker F, Wiebe J, Kastrati A, Cassese S, Xhepa E
J Cardiovasc Transl Res
· 2025 Oct · PMID 40588613
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In-stent restenosis (ISR) is the leading cause of revascularization failure, occurring in up to 10% of patients within 10 years after drug-eluting stent (DES) implantation, and is associated with increased mortality and...In-stent restenosis (ISR) is the leading cause of revascularization failure, occurring in up to 10% of patients within 10 years after drug-eluting stent (DES) implantation, and is associated with increased mortality and rehospitalization. Guideline-writing authorities recommend DES over drug-coated balloons (DCB) for ISR treatment. However, this indication is mainly based on trials that did not incorporate intravascular imaging. Recent findings suggest that optical coherence tomography (OCT) patterns in ISR may influence treatment outcomes. The ISAR-DESIRE 5 trial is a randomized study to evaluate whether OCT-defined ISR tissue morphology affects treatment with DES versus DCB. 376 patients with ISR will be stratified by OCT pattern (homogeneous vs. non-homogeneous) and randomized 1:1 to DES or DCB. The trial is powered to detect an interaction between OCT pattern and treatment modality on the 24-month incidence of major adverse cardiac events and represents a step toward establishing a more individualized approach to ISR management.Trial registration (ClinicalTrials.gov): NCT05544864.
Wadehra S, Singh S, Kaur S
… +2 more, Kaur A, Singh TG
J Cardiovasc Transl Res
· 2025 Dec · PMID 40560279
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Small Interfering RNA (siRNA) is a class of double-stranded, noncoding RNA that silences pathogenic mRNA through the process of RNA interference (RNAi). Its medical application is extensive, particularly in targeting gen...Small Interfering RNA (siRNA) is a class of double-stranded, noncoding RNA that silences pathogenic mRNA through the process of RNA interference (RNAi). Its medical application is extensive, particularly in targeting genes associated with cardiorenal diseases, including atherosclerosis, chronic kidney disease, hypertension and cardiac failure. The pathophysiology of cardiorenal syndrome is intricate, involving a network of neurohormonal, metabolic, hemodynamic, and inflammatory interactions. Thereby, this review emphasizes the mechanistic pathways and provides evidence from preclinical and clinical studies underscoring the therapeutic potential of siRNA in the cardiorenal axis. siRNA has been shown to alleviate hemodynamic stress, reduce inflammatory cytokines and disease biomarkers. Additionally, advancements in delivery systems are explored, with a focus on overcoming challenges such as poor stability, off-target effects and limited absorption to enhance clinical applicability. This review highlights the development of siRNA-based therapeutic strategies within the cardiorenal axis, emphasizing a molecular understanding of the underlying mechanisms.
Hørsdal OK, Frederiksen PH, Helgestad OKL
… +6 more, Ravn HB, Møller JE, Wiggers H, Nielsen RR, Gopalasingam N, Berg-Hansen K
J Cardiovasc Transl Res
· 2025 Oct · PMID 40555857
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The acute pathophysiological changes after myocardial ischemia complicated by cardiogenic shock (CS) remain poorly defined, especially regarding compensatory mechanisms and myocardial mitochondrial function. We investiga...The acute pathophysiological changes after myocardial ischemia complicated by cardiogenic shock (CS) remain poorly defined, especially regarding compensatory mechanisms and myocardial mitochondrial function. We investigated immediate cardiovascular and mitochondrial effects in a porcine model of ischemic CS. CS was induced in 32 Danish Landrace pigs (60 kg) via repeated microembolization of the left coronary artery until a 30% reduction in cardiac output (CO) or mixed venous saturation. Monitoring included pulmonary artery and left ventricular pressure-volume catheters, with analysis of endomyocardial biopsies and arterial, mixed venous, and coronary sinus blood samples. CO deteriorated promptly due to decreased stroke volume. Contractility declined, and afterload increased, causing rapid ventriculo-arterial decoupling. Forward flow parameters were compromised prior to pressure-parameters. Diastolic function was impaired and mitochondrial damage was observed. CS rapidly impairs LV hemodynamic and mitochondrial function, highlighting the importance of monitoring forward flow and targeting mitochondrial function in treatment.
Liu P, Ji L, Gan J
… +11 more, Yang X, Deng Y, Liao J, Mo P, Zhu Q, Jiang H, Han Y, Chen Z, Li W, Wu M, Zhu Y
J Cardiovasc Transl Res
· 2025 Aug · PMID 40553383
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The prognostic significance of the neutrophil-to-lymphocyte ratio (NLR) in relation to albumin levels for acute ischemic stroke (AIS) remains unclear. This study examined the correlation between inflammatory markers and...The prognostic significance of the neutrophil-to-lymphocyte ratio (NLR) in relation to albumin levels for acute ischemic stroke (AIS) remains unclear. This study examined the correlation between inflammatory markers and nutritional status to predict 90-day outcomes in patients with AIS. A total of 5855 patients with acute cerebral infarction admitted to the Stroke Center at Jiangsu Province Hospital of Chinese Medicine between June 2017 and September 2023 were retrospectively analyzed. The NLR, SIRI, SII, and NLR-ALb were calculated using peripheral venous blood indicators obtained 24 h post-admission. All patients were followed up 90 days after discharge, and their prognosis was evaluated using the modified Rankin Scale (mRS). Logistic regression analysis, receiver operating characteristics, and Kaplan-Meier survival curves were used to explore the association between inflammatory markers and 90-day outcomes in patients with AIS. In conclusion, higher levels of NLR, SIRI, SII, and the NLR-ALB ratio are associated with poor outcomes following AIS.
Wang Y, Liu W, Hao Y
… +5 more, Jia S, Hu M, Feng J, Nie Y, Bai L
J Cardiovasc Transl Res
· 2025 Aug · PMID 40553382
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Following myocardial infarction (MI), endothelial cell proliferation drives neovascularization to support cardiac regeneration. While myeloid-derived growth factor (Mydgf) is known to promote cardiac repair post-injury,...Following myocardial infarction (MI), endothelial cell proliferation drives neovascularization to support cardiac regeneration. While myeloid-derived growth factor (Mydgf) is known to promote cardiac repair post-injury, yet its mechanism in angiogenesis remained unclear. In this study, we found that myeloid-derived growth factor knockout (Mydgf-KO) reduced endothelial cell proliferation during early postnatal cardiac angiogenesis and impaired neovascularization post-MI, leading to exacerbated cardiac dysfunction. Recombinant MYDGF therapy reversed these deficits. Liquid chromatography mass spectrometry analysis (LC-MS) analysis of cardiac ECM in Mydgf-KO mice revealed FGF1 as a critical downstream effector of Mydgf in angiogenesis. Administration of recombinant FGF1 protein significantly promoted endothelial cell proliferation and tube formation in human coronary artery endothelial cells. These findings establish the Mydgf-FGF1 axis as a critical regulator of cardiac angiogenesis and unveil its promise as a therapeutic target to promote vascular repair in ischemic heart disease.
Kipanga ZG, Bao Z, Tella MG
… +9 more, Fiagbey EDK, Saad SH, Mbambara B, Bah CS, Iddi AK, Ding R, Si Y, Zhang Y, Zou J
J Cardiovasc Transl Res
· 2025 Aug · PMID 40553381
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Postoperative delirium (POD) is a common and severe complication following cardiac surgery, with an incidence of about 65% in older patients. This study aimed to develop a predictive model for POD in patients aged ≥ 18 y...Postoperative delirium (POD) is a common and severe complication following cardiac surgery, with an incidence of about 65% in older patients. This study aimed to develop a predictive model for POD in patients aged ≥ 18 years, to enhance early detection and intervention. A retrospective analysis was performed on the data collected from 825 patients who underwent cardiac surgery at Nanjing First Hospital (May 2021-July 2022). POD was diagnosed using the Confusion Assessment Method for the ICU (CAM-ICU). We used LASSO regression and multivariate logistic regression to identify key predictors for the nomogram. The incidence of POD was 23.6%, with significant predictors including head CT findings, hyperlactatemia before extubation, APACHE II score, ASA score, and length of hospital stay. The model demonstrated strong predictive ability (AUC: 0.922 training, 0.896 validation). A web-based calculator ( https://baozexiang.shinyapps.io/dynnomapp/ ) was developed to enhance clinical risk assessment and patient outcomes.
J Cardiovasc Transl Res
· 2025 Dec · PMID 40551060
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Cardiac surgery with cardiopulmonary bypass (CPB) may lead to many postoperative complications. However, many complications cannot be predicted in time using the current clinical methods, or the prediction is not suffici...Cardiac surgery with cardiopulmonary bypass (CPB) may lead to many postoperative complications. However, many complications cannot be predicted in time using the current clinical methods, or the prediction is not sufficiently accurate, resulting in a delay in treatment. Extracellular vesicles (EVs) are a group of membrane vesicles generated from various sources, including endothelial cells and platelets, upon activation or apoptosis. Here, we summarize the role of circulating EVs in predicting complications after cardiac surgery with cardiopulmonary bypass. We found that EV levels can predict acute heart failure. EV subtypes can predict acute heart failure, acute kidney injury, acute lung injury, blood transfusion, and neurological complications. The components of EVs can predict acute heart failure, acute kidney injury, acute lung injury, and neurological complications. The size distribution of EVs can predict acute lung injury. Therefore, circulating EVs can be used to predict complications after cardiac surgery with cardiopulmonary bypass.
Hussain K, Khurram S, Yousaf M
… +4 more, Ishtiaq A, Mushtaq I, Ali T, Murtaza I
J Cardiovasc Transl Res
· 2025 Dec · PMID 40551059
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Pathological growth of cardiomyocytes known as cardiac hypertrophy (CH). Differential expressions of miRNAs have an immense therapeutic potential against cardiac hypertrophy. The current study aim is to evaluate the ther...Pathological growth of cardiomyocytes known as cardiac hypertrophy (CH). Differential expressions of miRNAs have an immense therapeutic potential against cardiac hypertrophy. The current study aim is to evaluate the therapeutic potential of miRNA-137-3p/383-5p in cardiac hypertrophy by regulation of PGC-1α signaling nexus. Silencing of pro-hypertrophic miRNAs e.g. miR-137-3p and miR-383-5p leads to the restoration of their common target gene PGC-1α in hypertrophic cells. Interestingly, the results of this invivo study showed the cardioprotective effects of these antagomirs. Moreover, PGC-1α associated signaling events e.g. fatty acid oxidation (Cpt1a, Cpt1b), mitochondria membrane potential (MMP), mitochondrial reactive oxygen species (mtROS), oxidative phosphorylation (Ndufa6, Atp5me), apoptosis (Bcl-2, BAX), antioxidants (SOD, GSH, CAT), mitochondrial dynamic (Mfn-2, Drp-1) were significantly restored in the treated groups of miRNA antagomirs. Conclusively, this study uncovers that the pharmacological inhibition of miR-137-3p and miR-383-5p have a potential to rescue from the cardiac hypertrophy by regulation of PGC-1α signaling nexus.
Gong Y, Zhao C, Jia L
… +6 more, Qiao B, Tian J, Wen H, Wang Y, Yu B, Du J
J Cardiovasc Transl Res
· 2025 Oct · PMID 40540208
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The objective of this study was to identify plasma lipid signatures associated with plaque vulnerability. We retrospectively evaluated coronary plaque in 99 patients using optical coherence tomography (OCT) and quantifie...The objective of this study was to identify plasma lipid signatures associated with plaque vulnerability. We retrospectively evaluated coronary plaque in 99 patients using optical coherence tomography (OCT) and quantified 489 plasma lipids. We identified intra- and inter-class crosstalk among ceramide (Cer)-phosphatidylinositol (PI)-esterified cholesterol (CE)-sphingomyelin (SM) (Cer-PI-CE-SM) in patients with thin-cap fibroatheroma (TCFA). CE-16:0, SM d18:1/16:1, and GM3 d18:1/22:0, emerged as potential markers of TCFA, correlating with the thinnest fibrous cap thickness and the presence of cholesterol crystallization. Compared to the clinical model (area under the curve [AUC] = 0.810), the AUC of the combined clinical-lipid model improved [AUC = 0.880, p = 0.032]. Calibration and decision curves demonstrated that the combined model exhibited superior diagnostic performance. We identified lipid molecules that are strongly correlated with plaque vulnerability, thus providing an option for the non-invasive identification of vulnerable plaques, which could potentially facilitate the tailored treatment for high-risk patients.