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Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
Sun
200 papers
[Interpretation of the Disinfection Effects Testing and Evaluation Methods Section in ].
Wang Y, Wang J, Wei Q … +2 more , Yu L, Shen J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416146 · Full text
, a health industry standard, was officially released by the National Disease Control and Prevention Administration on December 15, 2023. The standard came into effect on May 1, 2024. This standard systematically specifi...
, a health industry standard, was officially released by the National Disease Control and Prevention Administration on December 15, 2023. The standard came into effect on May 1, 2024. This standard systematically specifies the testing and evaluation methods for disinfection products, covering three core components-disinfection effect testing and evaluation, physical and chemical testing techniques, and toxicological testing methods. The standard provides an important technical basis for the testing of disinfection products in China. To promote an accurate understanding and effective implementation of the standard, this article focuses on the in-depth interpretation of the section concerning the testing and evaluation methods of disinfection effects. It provides a detailed explanation of the major updates, technical highlights, and scientific rationale behind the standard. The article incorporates discussions on optimizing the validation test methods for the disinfection and sterilization effects of disinfectants, simulated field testing of disinfectants, evaluation of air disinfection effects, and the supplementation and improvement of testing methods for disinfection devices (including sterilization devices). This article aims to provide clear technical guidance for disinfection product inspection personnel, researchers, and other professionals involved, promote the standardized application of the standard, improve the quality of disinfection products, and ensure scientific, effective, and safe disinfection practices.
[Molecular Epidemiology and Fluconazole Resistance Mechanisms in Isolates From Bloodstream Infections in Sichuan, China].
Chen W, Hou J, Peng L … +2 more , Kang M, Ma Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416145 · Full text
OBJECTIVE: To analyze the molecular epidemiology of () isolates from bloodstream infections, and to investigate preliminarily the underlying mechanisms of fluconazole resistance. METHODS: Clinical isolates were collect...
OBJECTIVE: To analyze the molecular epidemiology of () isolates from bloodstream infections, and to investigate preliminarily the underlying mechanisms of fluconazole resistance. METHODS: Clinical isolates were collected from bloodstream infections at West China Hospital, Sichuan University and other hospitals in Sichuan Province. All the collected isolates were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and the antifungal susceptibility test was performed by the broth microdilution method. Multilocus sequence typing (MLST) was conducted using the first generation sequencing technique. Genes associated with drug-resistance were sequenced and the mutation sites were identified. The relative expression levels of genes associated with drug-resistance were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). RESULTS: A total of 39 isolates from bloodstream infections were collected. The MLST phylogenetic tree showed that most of the strains clustered in the phylogenetic tree shared identical or similar minimal inhibitory concentrations (MICs). All MLST types were not matched with the isolates in the PubMLST database, representing new diploid sequence types (DSTs). A total of 3 sense mutations, including Ser154Phe, Tyr132Phe, and Tyr257His were identified in , and were present only in drug-resistant strains. contained 4 sense mutations, including Ala251Thr, Gln289Leu, Ser279Glu, and Gln313His, which were also present only in drug-resistant strains. Ser279Glu and Gln313His were previously unreported. There were two sense mutations in ERG3, namely Ser112Gly and Ala48Ser, both being previously unreported, and Ser112Gly was found only in drug-resistant strains. The expression levels of (14.48 vs 7.109, = 0.003) and (1.922 vs 0.832, = 0.04) in non-sensitive group (MIC > 2 mg/L) were higher than those in sensitive group (MIC ≤ 2 mg/L), and the difference was statistically significant. CONCLUSION: There may be a unique genetic relationship among isolates from Sichuan province, and the MICs of fluconazole against are associated with molecular typing. ERG11 mutations were the major mechanism underlying fluconazole resistance in isolates. The upregulation of and was associated with fluconazole resistance in .
[Multimodal Data-Driven Prediction of Gynecological Surgery Duration].
Huang Y, Yong Z, Wu B … +6 more , Zhou X, Lang X, Li Y, Wang M, Shi Q, Rao L
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416144 · Full text
OBJECTIVE: Focusing on gynecological surgery, we constructed a prediction model for surgical duration by extracting features from unstructured surgical planning texts and integrating multimodal data via artificial intell...
OBJECTIVE: Focusing on gynecological surgery, we constructed a prediction model for surgical duration by extracting features from unstructured surgical planning texts and integrating multimodal data via artificial intelligence technology. METHODS: The clinical data of 34614 patients who underwent gynecologic surgeries at West China Second University Hospital, Sichuan University between January 2022 and October 2024 were collected. An embedding-transformer model was constructed to convert surgical planning texts into a one-dimensional numerical feature, referred to as the step feature. The predictive value of the step feature was assessed by comparing the performance improvements of linear regression, random forest, eXtreme Gradient Boosting (XGBoost), support vector regression, K-nearest neighbor regression, and artificial neural network algorithms in two scenarios-with and without the step feature as an input. The out-of-sample prediction accuracy of the models was assessed using mean absolute error (MAE), root mean squared error (RMSE), and R-squared ( ). Furthermore, the model interpretability was examined using SHapley Additive exPlanations (SHAP) values. RESULTS: SHAP results showed that the step feature had the highest predictive contribution. Temporal factors in surgical scheduling also influenced gynecological surgery duration. The XGBoost model demonstrated optimal performance on the test set, significantly improving prediction accuracy with a 40.43% increase in , while reducing MAE and RMSE by 21.27% and 20.13%, respectively, compared to the baseline model without the step feature. CONCLUSION: The embedding-transformer model developed in this study effectively extracts features from surgical planning texts and enhances the predictive performance of machine learning models. The XGBoost prediction model can assist hospital administrators in implementing more refined management of gynecological surgeries and improving the utilization efficiency of surgical resources.
[Association of Myelin Basic Protein and S100 Calcium-Binding Protein B With the Risk of Early Neurological Deterioration After Interventional Therapy for Cerebral Infarction].
Yan H, Ren J, Wu Q … +1 more , Wang W
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416143 · Full text
OBJECTIVE: To investigate and analyze the association of serum levels of myelin basic protein (MBP) and S100 calcium-binding protein B (S100-B) with early neurological deterioration in patients with cerebral infarction a...
OBJECTIVE: To investigate and analyze the association of serum levels of myelin basic protein (MBP) and S100 calcium-binding protein B (S100-B) with early neurological deterioration in patients with cerebral infarction after interventional therapy. METHODS: A total of 258 patients with cerebral infarction, admitted to our hospital between July 2021 and July 2024, were enrolled. The National Institutes of Health Stroke Scale (NIHSS) was used to evaluate the neurological function status of patients. Patients who died or whose NIHSS score increased by 4 points or more after 24 hours of interventional therapy were included in the early neurological deterioration group, while the remaining patients were included in the non-deterioration group. The levels of MBP and S100-B in all patients were measured, and the relationship between changes in MBP and S100-B levels and the risk of neurological deterioration after interventional therapy was analyzed. RESULTS: The levels of serum MBP and S100-B of patients with cerebral infarction were markedly higher in the early neurological deterioration group compared with those in the non-deterioration group ( = 9.062 [95% CI: 2.348-3.663] and 7.708 [95% CI: 0.221-0.375], < 0.001). Spearman correlation analysis showed that the levels of serum MBP and S100-B in the deterioration group were positively correlated with NIHSS score increase ( = 0.323 [95% CI: 0.095-0.542] and 0.292 [95% CI: 0.066-0.488], < 0.05). Stratified regression analysis showed that serum MBP (OR = 1.996, 95% CI: 1.607-2.478) and S100-B (OR = 1.005, 95% CI: 1.003-1.007) were risk factors affecting the early deterioration of neurological function in patients with cerebral infarction ( < 0.05), even after adjusting for confounding factors. In addition, admission NIHSS score (OR = 1.224, 95% CI: 1.142-1.310), hypertension (OR = 2.542, 95% CI: 1.139-5.669) and hyperlipidemia (OR = 2.618, 95% CI: 1.101-6.228) were also risk factors, and an interaction effect between the admission NIHSS score and MBP (OR = 1.081, 95% CI: 1.034-1.130) was observed. Receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) of serum MBP and S100-B in evaluating early neurological deterioration were 0.822 (95% CI: 0.764-0.879) and 0.788 (95% CI: 0.724-0.853), respectively. CONCLUSION: Elevated levels of serum MBP and S100-B in patients with cerebral infarction after interventional therapy are associated with an increased risk of early neurological deterioration and show potential value for assessing the risk of neurological deterioration.
[Predictive Performance of Routine Blood Test Parameters for the Severity of Brain Damage in Patients With Acute Cerebral Hemorrhage in High-Altitude Regions].
Ma J, Liu B, Renqinglamu … +4 more , Li R, Zheng W, Zhao Y, Xiong H
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416142 · Full text
OBJECTIVE: To investigate the predictive value of changes in various indicators of routine blood tests for the severity of brain damage in patients with acute cerebral hemorrhage (ACH) in high-altitude regions. METHODS:...
OBJECTIVE: To investigate the predictive value of changes in various indicators of routine blood tests for the severity of brain damage in patients with acute cerebral hemorrhage (ACH) in high-altitude regions. METHODS: A retrospective analysis was conducted using data from 249 inpatients diagnosed with ACH at a hospital in Xizang. Patient data, including demographic information and results of routine blood tests, were collected. Based on their Glasgow Coma Scale (GCS) scores, the patients were divided into 2 groups, a mild brain damage group (GCS ≥ 13 points) and a moderate-to-severe brain damage group (GCS ≤ 12 points). Demographic information and laboratory test results were compared between the two groups. Binary logistic regression analysis was performed to examine the relationship between indicators of routine blood tests and the severity of brain damage, and key indicators were identified. The receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive performance of key indicators in various combinations for the severity of brain injury in ACH patients. RESULTS: The moderate-to-severe group had a higher proportion of high-altitude residents, as well as elevated levels of white blood cells (WBC), neutrophils (N), neutrophil-to-lymphocyte ratio (NLR), systemic inflammatory index (SII), neutrophil-to-platelet ratio (NPR), and C-reactive protein (CRP)-to-lymphocyte ratio (CLR), compared to the mild group. On the other hand, the levels of lymphocytes (L), platelets (PLT), and platelet-to-albumin ratio (PAR) in the moderate-to-severe group were lower than those in the mild group. Binary logistic regression analysis showed that WBC (OR = 1.221, 95% CI: 1.127-1.322), CRP (OR = 1.019, 95% CI: 1.004-1.033), CRP-to-albumin ratio (CAR) (OR = 1.845, 95% CI: 1.137-2.996), and CLR (OR = 1.018, 95% CI: 1.005-1.030) were positively associated with moderate-to-severe brain damage, and that their elevated levels were associated with an increase in risk by the corresponding folds. PAR (OR = 0.845, 95% CI: 0.721-0.990) was negatively correlated with moderate-to-severe brain damage, with an increase resulting in a reduction of risk by 0.845 times. ROC curve analysis showed that, in the mild group, the combined AUC for WBC + CRP was 0.689 (optimal cutoff value 0.19, specificity 0.776, 95% CI: 0.624-0.755) ; the PAR + CAR + CLR combination (CAR was not significant, > 0.05) had an AUC of 0.675 (optimal cutoff value 0.19, specificity 0.760, 95% CI: 0.609-0.741); the key indicator combination of WBC + PAR + CLR (CRP was not significant, > 0.05) demonstrating the strong predictive performance moderate-to-severe brain damage, yielding an AUC of 0.737 (optimal cutoff value 0.08, specificity 0.624, 95% CI: 0.676-0.798). CONCLUSION: The indicator combinations of WBC + CRP, PAR + CLR, and WBC + PAR + CLR exhibit significant value for predicting the severity of brain injury in ACH patients and may serve as potential predictive tools for the severity of brain damage in patients with acute cerebral hemorrhage in high-altitude regions.
[Molecular Mechanisms of Intervention With Lishukang Capsule in a Rat Model of High-Altitude Pulmonary Edema].
Zhang D, Yang C, Li X … +3 more , Shao J, Li W, Wang R
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416141 · Full text
OBJECTIVE: To investigate the molecular targets and signaling pathways involved in the therapeutic effects of Lishukang Capsule (LSK) in a rat model of high-altitude pulmonary edema (HAPE) using a proteomics-based approa...
OBJECTIVE: To investigate the molecular targets and signaling pathways involved in the therapeutic effects of Lishukang Capsule (LSK) in a rat model of high-altitude pulmonary edema (HAPE) using a proteomics-based approach. METHODS: A total of 60 male Wistar rats were randomly assigned to a control group, a HAPE model group, 3 LSK treatment groups receiving low-, medium-, and high-dose LSK, respectively, and a (also known as Hongjitian [HJT] in pinyin, a Chinese Romanization system) control group. After HAPE modeling, the pharmacodynamic effects were assessed and the optimal LSK dose was determined using HE stains, inflammatory cytokine quantification, lung tissue water content, and the protein concentration in bronchoalveolar lavage. Label free quantitative proteomic profiling was then applied to identify differentially expressed proteins (DEPs) in the optimal dose group, using a screening threshold of over 1.5-fold change and < 0.05. The selected DEPs were validated with Western blotting, followed by Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. RESULTS: The medium-dose LSK group exhibited significant anti-HAPE effects. Findings from the proteomic analysis revealed, in the comparison with the control group, 267 DEPs were identified in the HAPE group. In the comparison with the HAPE group, 225 DEPs were identified in the medium-dose LSK group. A total of 112 DEPs in the control group were normalized following LSK treatment in the medium-dose LSK group. In addition, GO enrichment analysis of proteins differentially expressed between the HAPE and LSK group showed that these DEPs were mainly enriched in 12 biological processes, 2 cellular components, and 5 molecular functions. KEGG pathway analysis showed that LSK activated pathways associated with cell adhesion molecules, glycosaminoglycan biosynthesis, DNA replication/nucleotide excision repair, transcriptional dysregulation in cancer, and Herpes simplex virus type 1 (HSV-1) infection, while inhibiting pathways associated with glycerophospholipid metabolism. Some differentially expressed proteins with potential functions were verified by Western blotting, including AGPAT5, NCAM1, SRSF3, and PLA2. These differentially expressed proteins were significantly expressed in the normal group, HAPE group, and LSK group, and the validation results were consistent with proteomic findings, indicating the high reliability of the proteomic results. CONCLUSION: LSK exerts a significant protective effect against HAPE. Proteomic analysis suggests that its therapeutic action may be mediated through activating pathways involved in cell adhesion molecules, glycosaminoglycan biosynthesis, DNA replication/nucleotide excision repair, transcriptional dysregulation in cancer, and HSV-1 infection, alongside inhibition of pathways associated with glycerophospholipid metabolism. The key DEPs identified in these pathways may play crucial roles in the preventive and therapeutic effects of LSK on HAPE.
[Association Between Social Anxiety Symptoms and Brain Metabolism].
Zhao Z, Shen G, Chen T … +5 more , Xie H, Gan R, Wang M, Sun H, Jia Z
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416140 · Full text
OBJECTIVE: In this study, we investigated the correlation between the scores for different Liebowitz Social Anxiety Scale (LSAS) subscale models and the metabolic activity in specific regions of the brain using F-fluorod...
OBJECTIVE: In this study, we investigated the correlation between the scores for different Liebowitz Social Anxiety Scale (LSAS) subscale models and the metabolic activity in specific regions of the brain using F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) (F-FDG PET), thereby improving the understanding of the neurobiological characteristics of social anxiety. METHODS: A total of 39 cognitively normal participants (29 men and 10 women, aged 30-63 years) were enrolled. All participants underwent LSAS assessment and brain F-FDG PET scanning. Correlations between metabolic activities in various brain regions and scores from the different LSAS subscales were analyzed accordingly. RESULTS: LSAS subscale scores were significantly correlated with metabolic activity in specific brain regions. In the Safren model, the score for the observation by others subscale was positively correlated with the left fusiform gyrus ( < 0.001, false discovery rate [FDR]-corrected) and the left caudate tail ( < 0.001, FDR-corrected), suggesting a close association between mood states related to observation by others and the metabolic activity in these regions. In the Baker model, the score for the eating and drinking subscale was negatively correlated with the right precuneus ( < 0.001, FDR-corrected), while the score for the assertiveness subscale was positively correlated with the left caudate nucleus ( < 0.001, FDR-corrected). These findings revealed the complex associative patterns between various mood and behavioral dimensions and metabolic activities in specific brain regions. CONCLUSION: Social anxiety symptoms are closely associated with metabolic changes in specific brain regions, including the left insula, left caudate tail, and right precuneus. Moreover, different social situations activate distinct brain regions. Compared with individuals with social anxiety disorder, normal individuals exhibit involvement of fewer brain regions when experiencing social anxiety. These findings provide new experimental evidence for understanding the neural mechanisms underlying social anxiety.
[Inhibitory Effects of the Deep Underground Low Background Radiation Environment on the Proliferation and Migration of NP69 Human Nasopharyngeal Epithelial Cells and the Underlying Mechanisms].
Hao Z, Li C, Wang L … +6 more , Zou Y, Wen J, Wu J, Wan X, Liu F, Liu J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416139 · Full text
OBJECTIVE: To investigate the effects of low background radiation environments in deep underground settings on the biological behavior of NP69 human nasopharyngeal epithelial cells (NP69 cells) and the underlying molecul...
OBJECTIVE: To investigate the effects of low background radiation environments in deep underground settings on the biological behavior of NP69 human nasopharyngeal epithelial cells (NP69 cells) and the underlying molecular mechanisms. METHODS: A parallel control experimental design was adopted and NP69 cells were synchronously cultured in settings of three underground depths at the China Deep-Underground Facility & Life Observatory (DeUFO)-ground level (DeUFO-0 m), 1000 m underground (DeUFO-1000 m), and 1500 m underground (DeUFO-1500 m). Changes in cell proliferation and migration capabilities were assessed using the Cell Counting Kit-8 (CCK-8) assay and scratch assay, respectively. High-throughput RNA sequencing (RNA-Seq) was performed to identify differentially expressed genes (DEGs). Functional annotation and pathway enrichment analysis of the DEGs were performed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. RESULTS: CCK-8 assay revealed that, after 72 h of culture, the absorbance value of the DeUFO-0 m group was 1.35 times and 1.27 times those of the those of the DeUFO-1000 m and DeUFO-1500 m groups, respectively (both < 0.0001). After 96 h of culture, the absorbance value of the DeUFO-0 m group was 1.52 times and 1.41 times those of the DeUFO-1000 m and DeUFO-1500 m groups, respectively (both < 0.0001). Colony formation assays revealed that the number of cell colonies in the DeUFO-0 m group was 1.59 times and 1.27 times those in the DeUFO-1000 m group and DeUFO-1500 m group, respectively (both < 0.001). The scratch assay revealed that the 36-hour wound healing rate of the DeUFO-0 m group was 2.22 times and 4.00 times those of the DeUFO-1000 m group and DeUFO-1500 m group, respectively (both < 0.0001). Transwell assays revealed that the number of migrating cells in the DeUFO-0 m group was 2.08 times and 2.56 times those in the DeUFO-1000 m group and DeUFO-1500 m group, respectively (both < 0.0001). Transcriptome sequencing analysis revealed consistent upregulation of , , , , and genes in the DeUFO-1000 m and DeUFO-1500 m groups. Pathway enrichment analysis indicated significant enrichment of extracellular matrix (ECM) remodeling-associated pathways and gene expression regulation pathways in the experimental groups (false discovery rate [FDR] < 0.05). CONCLUSION: The low background radiation environment in deep underground settings suppresses the proliferation and migration activities of NP69 cells by mediating ECM remodeling and post-transcriptional regulatory mechanisms through the regulation of target genes such as the CELF family. This study provides experimental evidence for establishing a dose-response relationship between environmental radiation and cellular effects.
[Advances in Nanozyme-Aptamer Systems for the Detection of Foodborne Pathogens].
Liang H, Jia S, Zhan Z … +2 more , Cai Y, Niu X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416138 · Full text
Food safety problems caused by foodborne pathogenic bacteria pose a serious threat to public health, creating an urgent need to develop testing methods and techniques with excellent performance and are simple to use and...
Food safety problems caused by foodborne pathogenic bacteria pose a serious threat to public health, creating an urgent need to develop testing methods and techniques with excellent performance and are simple to use and of affordable cost. Traditional testing methods, such as isolation and culture, morphological observation, biochemical identification, and serological tests, have many limitations, including complex procedures, reliance on specialized technical equipment and personnel, and long turnaround time, rendering them inadequate for meeting current and future testing demands. Therefore, it is particularly important to develop simple, rapid, and highly sensitive methods for analyzing pathogenic bacteria. The fusion of nucleic acid aptamers and nanozymes brings new ideas for the rapid testing of pathogenic bacteria. On one hand, aptamers offer specific recognition capability for target bacteria and can be combined with various nucleic acid signal amplification techniques. On the other hand, the enzyme-like catalytic activity and signal amplification effect of many nanomaterials provide a basis for highly sensitive testing. This review highlights the application potential of nanozyme‒aptamer coupling systems in the field of microbial analysis by briefly summarizing the latest research progress in the use of nanozymes combined with aptamers for the detection of foodborne pathogenic bacteria. First of all, two main approaches to conjugating nanozymes with aptamers are introduced. Then, the testing mechanisms and typical applications of nanozyme‒aptamer coupling systems for foodborne pathogenic bacteria are discussed. Finally, future development trends and existing challenges are disucssed from four perspectives, including specificity, high sensitivity, high throughput, and intelligent detection. This review aims to provide a useful reference for the fusion of nanozymes and aptamers and for the development of on-site rapid testing techniques for foodborne pathogens, and to encourage broader academic interest to further advance this promising research field.
[Simultaneous Analysis of Microcystins, Cylindrospermopsin, Anatoxin, and Nodularin in Lake Water by Liquid Chromatography-Tandem Mass Spectrometry].
Yuan G, Luo Q, Liu G … +4 more , Qin X, Liu H, Lü Z, Jiang J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Sep · PMID 41416137 · Full text
OBJECTIVE: To establish a method for simultaneous determination of trace levels of microcystins, cylindrospermopsin, anatoxin, and nodularin in lake water based on liquid chromatography-tandem mass spectrometry (LC-MS/MS...
OBJECTIVE: To establish a method for simultaneous determination of trace levels of microcystins, cylindrospermopsin, anatoxin, and nodularin in lake water based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). METHODS: After being adjusted to alkaline conditions and mixed with six internal standards, the water samples were enriched using dual HLB and ENVI-Carb cartridges. The eluates were then evaporated under nitrogen, reconstituted, and subjected to instrumental analysis. Both water and acetonitrile containing 0.1% formic acid were used as mobile phases. An ACQUITY UPLC® BEH C column (150 mm × 2.1 mm, 1.7 μm) was selected to separate the target cyanotoxins. Multiple reaction monitoring was applied for data acquisition, and quantification was accomplished using internal standard methods. RESULTS: Within certain concentration ranges, all 14 cyanotoxins examined in the study showed good linearity, with all correlation coefficients greater than 0.998. When the water volume was 100 mL, the limits of detection and quantification for the 14 cyanotoxins were 0.1-0.9 ng/L and 0.3-2.9 ng/L, respectively, and spiked recoveries and relative standard deviations were 81.7%-132.9% and 1.2%-14.9%, respectively. In the 10 lake water samples analyzed, cylindrospermopsin, anatoxin-α, and multiple microcystins were detected. CONCLUSION: The method developed in the study has high-throughput capacity, as well as high sensitivity, accuracy, and reliability. The method can be applied in the simultaneous detection of microcystins, cylindrospermopsin, anatoxin, and nodularin in lake water.
[Salidroside Inhibits the Proliferation of Gastric Cancer Cells by Regulating the miR-1343-3p/SOX18 Signaling Axis].
Zhang Z, Cao X, Hou X … +5 more , Cao M, DU Y, Zhang J, Sun Y, Wang X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113433 · Full text
OBJECTIVE: To investigate the molecular mechanism by which salidroside inhibits the proliferation of gastric cancer (GC) cells through upregulation of miR-1343-3p. METHODS: RNA databases were used to screen for mRNAs ass...
OBJECTIVE: To investigate the molecular mechanism by which salidroside inhibits the proliferation of gastric cancer (GC) cells through upregulation of miR-1343-3p. METHODS: RNA databases were used to screen for mRNAs associated with tumor proliferation and with miR-1343-3p, and exhibiting significant changes in their expression levels after salidroside treatment of human GC cells. Gene matching and immunoprecipitation of RNA-binding proteins were conducted to analyze the association between miR-1343-3p and SOX18. Immunocytochemistry was performed to determine the localization of SOX18 protein. The effect of salidroside on the proliferation of human GC cells (MGC-803 and AGS) was determined by CCK-8 assay. Human GC cells were divided into a blank control group and low- and high-dose salidroside groups. The expression of miR-1343-3p and mRNA was measured by real-time quantitative fluorescence PCR (qPCR). The protein expression of SOX18 was measured by Western blot. GC cells were co-transfected with miR-1343-3p mimic and miR-1343-3p inhibitor, respectively, via Lipofectamine 2000 liposomes. The expression of miR-1343-3p and mRNA was measured by qPCR, and the protein expression of SOX18 was measured by Western blot. RESULTS: Through bioinformatic analysis, was identified as a downstream target of miR-1343-3p. Gene alignment confirmed the presence of specific binding sites between the two genes, and immunoprecipitation of RNA-binding proteins validated the targeting relationship between them ( < 0.05). Immunocytochemistry demonstrated the nuclear localization of SOX18 protein. CCK-8 assay findings demonstrated that salidroside significantly inhibited the proliferation of GC cells in a time- and dose-dependent manner. Compared with the blank control group, salidroside-treated GC cells showed decreased expression of both mRNA and protein ( < 0.05) and an increased miR-1343-3p expression ( < 0.05). Compared with the control group, GC cells in the miR-1343-3p mimic group exhibited increased expression of miR-1343-3p and decreased expression of mRNA and protein. In contrast, GC cells in the miR-1343-3p inhibitor group showed decreased expression of miR-1343-3p and increased expression of SOX18 mRNA and protein (all < 0.05). CONCLUSION: Salidroside may inhibit the proliferation of GC cells by regulating the miR-1343-3p/SOX18 signaling axis and these regulators may present new potential therapeutic targets or biomarkers for gastric cancer.
[Research Advancements in Gastric Capacity Intervention for Childhood and Adolescent Obesity].
Zhou A, Yang F
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113432 · Full text
The global prevalence of obesity among children and adolescents is rising rapidly, and the long-term comorbidities of obesity pose serious threats to the population health and life expectancy. Interventions for childhood...
The global prevalence of obesity among children and adolescents is rising rapidly, and the long-term comorbidities of obesity pose serious threats to the population health and life expectancy. Interventions for childhood and adolescent obesity mainly include lifestyle and psycho-behavioral approaches. Pharmacological treatment and metabolic bariatric surgery are subject to strict clinical indications and, hence, have limited clinical application. In recent years, the concept of intragastric volume occupancy has emerged as a promising approach to the clinical management of childhood and adolescent obesity, for it has demonstrated notable weight-loss efficacy, broad applicability across different populations, a favorable safety profile, and sustained long-term effectiveness. Herein, we systematically evaluated the advantages and limitations of existing intervention strategies for childhood and adolescent obesity. We discussed the mechanisms of action, clinical application progress, and evaluation of weight loss effects of intragastric volume occupancy intervention methods. Further multicenter, large-sample studies are warranted to systematically elucidate the regulatory mechanisms of intragastric occupancy technology on the gut microbiota of children and adolescents, and to establish an evidence-based foundation for its translation into clinical practice for obesity intervention, accordingly.
[Expression Levels and Clinical Significance of Serum IL-4, IL-5, IL-6, IL-13 and IL-17 in Children With Infection Accompanied by Airway Hyperresponsiveness].
Jin Y, Zhu M, Song L
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113431 · Full text
OBJECTIVE: To analyze the expression levels and the clinical significance of serum interleukin (IL)-4, IL-5, IL-6, IL-13 and IL-17 in children with mycoplasma pneumoniae (MP) infection accompanied by airway hyperresponsi...
OBJECTIVE: To analyze the expression levels and the clinical significance of serum interleukin (IL)-4, IL-5, IL-6, IL-13 and IL-17 in children with mycoplasma pneumoniae (MP) infection accompanied by airway hyperresponsiveness (AHR). METHODS: A total of 120 children diagnosed with MP infection pneumonia and admitted to Nantong First People's Hospital between June 2022 and April 2024 were enrolled in the study group. According to whether their MP infection pneumonia was accompanied by AHR, the participants were divided into an AHR group ( = 41) and a non-AHR group ( = 79). An additional 90 healthy children undergoing physical examination were included in the control group. Baseline data, fractional exhaled nitric oxide (FeNO), tidal breathing lung function-including the ratio of volume at peak tidal expiratory flow to total expiratory volume (VPTEF/VE), the ratio of time to peak tidal expiratory flow to total expiratory time (TPTEF/TE), and inspiratory-to-expiratory time ratio (TI/TE)-as well as the levels of serum IL-4, IL-5, IL-6, IL-13 and IL-17 were compared among the 3 groups. The correlation between serum IL-4, IL-5, IL-6, IL-13 and IL-17 and clinical data of children with MP infection pneumonia accompanied by AHR was analyzed. Logistic regression analysis was used to identify the independent influencing factors of AHR in children with MP infection pneumonia. Receiver operating characteristic (ROC) curves were plotted to assess the diagnostic value of serum IL-4, IL-5, IL-6, IL-13 and IL-17 for MP infection pneumonia accompanied by AHR in children. RESULTS: Significant differences were observed among the AHR, non-AHR, and control groups in white blood cell (WBC) count, neutrophil percentage, eosinophil (EOS) count, platelet (PLT) count, C-reactive protein (CRP), FeNO, VPTEF/VE, TPTEF/TE, TI/TE, forced vital capacity (FVC), and forced expiratory volume in one second (FEV₁) (all < 0.05).Serum IL-4, IL-5, IL-6, IL-13 and IL-17 levels showed significant differences among the 3 groups ( < 0.05). Correlation analysis showed that these cytokines were positively correlated with WBC count, neutrophil percentage, EOS count, CRP, and FeNO ( < 0.05), and negatively correlated with VPTEF/VE, TPTEF/TE, TI/TE, FVC, and FEV ( < 0.05). Binary logistic regression analysis identified neutrophil percentage (odds ratio [OR] = 1.923; 95% CI, 1.496-2.472), EOS count (OR = 3.074; 95% CI, 1.228-7.693), CRP (OR = 2.382; 95% CI, 1.854-3.061), FeNO (OR = 1.931; 95% CI, 1.635-2.281), VPTEF/VE (OR = 0.294; 95% CI, 0.200-0.432), TPTEF/TE (OR = 0.358; 95% CI, 0.177-0.722), TI/TE (OR = 0.399; 95% CI, 0.221-0.722), IL-4 (OR = 1.064; 95% CI, 1.019-1.111), IL-5 (OR = 1.234; 95% CI, 1.095-1.390), IL-6 (OR = 1.013; 95% CI, 1.001-1.025), IL-13 (OR = 1.058; 95% CI, 1.005-1.113), IL-17 (OR = 1.759; 95% CI, 1.293-2.393), and allergy history (OR = 2.989; 95% CI, 1.058-8.447) as independent factors associated with with AHR in children with MP infection pneumonia ( < 0.05). ROC curves revealed that the areas under the ROC curves (AUC) of serum IL-4, IL-5, IL-6, IL-13 and IL-17 for predicting AHR in children with MP infection pneumonia were 0.815, 0.769, 0.782, 0.793, and 0.815, respectively, with the sensitivities being 85.37%, 75.61%, 87.80%, 75.61%, and 80.49%, and 95% CI being 0.739-0.892, 0.677-0.861, 0.679-0.867, 0.715-0.882 and 0.732-0.899, respectively. CONCLUSION: The levels of serum IL-4, IL-5, IL-6, IL-13, and IL-17 in children with MP infection pneumonia and AHR are abnormally elevated. These cytokines may serve as valuable biomarkers for assessing the risk of AHR in children with MP infection pneumonia.
[Application Value of Pancreatic Duct-to-Jejunum Stent-Bridging Internal Drainage in Robotic Central Pancreatectomy].
Zhang E, Wu J, Chen M … +5 more , Ma B, Wu H, Xu Y, Cui H, Zhao Z
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113430 · Full text
OBJECTIVE: To conduct preliminary evaluation of the reliability and safety of pancreatic duct-to-jejunum stent-bridging internal drainage as a supplementary approach to pancreaticojejunostomy in central pancreatectomy. M...
OBJECTIVE: To conduct preliminary evaluation of the reliability and safety of pancreatic duct-to-jejunum stent-bridging internal drainage as a supplementary approach to pancreaticojejunostomy in central pancreatectomy. METHODS: The clinical data of 28 patients who underwent robotic central pancreatectomy performed by our team between January 2021 and November 2024 were retrospectively collected, and and follow-up of postoperative endocrine and exocrine functions was performed. Based on the methods of digestive tract reconstruction adopted, the patients were divided into a conventional pancreaticojejunostomy group and a pancreatic duct-to-jejunum stent-bridging internal drainage group (PancreaticoJejunal-Stent bridge group). The operative time, digestive tract reconstruction time, and short-term complications were compared between the two groups. RESULTS: Among patients undergoing robotic central pancreatectomy, the digestive tract reconstruction time was shorter ( = 5.168, < 0.001) in the PancreaticoJejunal-Stent bridge group ([31.1 ± 6.3] min) than that in the conventional pancreaticojejunostomy group ([49.7 ± 8.9] min) ( = 5.168, < 0.001). The total operative time was (172.7 ± 64.6) min in the PancreaticoJejunal-Stent bridge group and (200.1 ± 52.7) min in the conventional pancreaticojejunostomy group, showing no statistically significant difference ( = 1.215, = 0.235). In the PancreaticoJejunal-Stent bridge group, one patient developed a postoperative biochemical fistula, and 14 patients developed grade B pancreatic fistulas. Among the 14 patients with grade B pancreatic fistulas, 1 case was complicated by fistula-related intra-abdominal infection, and 13 cases had drainage tube retention time of more than 21 days. In the conventional pancreaticojejunostomy group, 2 patients developed postoperative biochemical fistulas, and 11 patients developed grade B pancreatic fistulas. Among the 11 patients with grade B pancreatic fistulas, 1 case was complicated by fistula-related intra-abdominal infection, and 1 case was complicated by fistula-related intra-abdominal bleeding and infection. No postoperative gastroparesis, pancreatitis, or grade C pancreatic fistulas occurred in either group. There were no statistically significant differences between the two groups in overall postoperative complication rate ( = 0.522), postoperative pancreatic fistula rate ( = 0.583), intra-abdominal infection rate ( = 0.583), or bleeding rate ( = 0.464). CONCLUSION: Pancreatic duct-to-jejunum stent-bridging internal drainage optimizes the anastomosis between the distal end of the pancreas and the jejunum during central pancreatectomy, shortens digestive tract reconstruction time, and reduces surgical complexity without increasing the risk of short-term severe postoperative complications. This approach is safe and feasible.
[Efficacy of Transfer Energy Capacitive and Resistive Therapy Combined With β-Hydroxy-β-Methylbutyrate Nutritional Supplementation in Older Adults With Sarcopenic Obesity].
Wang R, Liang Y, Wang L … +2 more , Yang M, Jiang J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113429 · Full text
OBJECTIVE: To investigate the comprehensive intervention effects of transfer energy capacitive and resistive (TECAR) therapy combined with β-hydroxy-β-methylbutyrate (HMB) nutritional supplementation in older patients wi...
OBJECTIVE: To investigate the comprehensive intervention effects of transfer energy capacitive and resistive (TECAR) therapy combined with β-hydroxy-β-methylbutyrate (HMB) nutritional supplementation in older patients with sarcopenic obesity (SO). METHODS: We conducted a randomized controlled trial, enrolling 140 older patients who met the Asian diagnostic criteria for SO. Participants were randomly assigned to 4 groups, including a double-placebo group (Group A), TECAR + placebo group (Group B), sham TECAR + HMB group (Group C), and TECAR + HMB group (Group D), with 35 patients in each group. The intervention lasted 12 weeks. The primary outcome measure was the total score of the Short Physical Performance Battery (SPPB). Secondary outcome indicators included the modified Barthel Index (MBI), scores of the Mini Nutritional Assessment-Short Form (MNA-SF), handgrip strength, body mass, and body mass index (BMI). A two-way analysis of variance (ANOVA) was used to assess the interaction effects between TECAR and HMB. RESULTS: After the intervention, Group D (TECAR + HMB) demonstrated significant improvements across all metrics. The SPPB total score increased from 6.29 ± 1.34 to 8.06 ± 1.51 ( < 0.001), with notable enhancements in walking speed (2.71 ± 0.86 vs. 1.97 ± 0.82), chair stand (2.60 ± 0.55 vs. 2.11 ± 0.47), and balance (2.74 ± 0.74 vs. 2.20 ± 0.76). MBI improved from 71.74 ± 14.41 to 79.91 ± 10.52 ( < 0.001). Handgrip strength increased from (13.65 ± 5.05) kg to (15.72 ± 4.89) kg ( = 0.001). Body mass decreased from (81.78 ± 9.02) kg to (76.95 ± 9.89) kg ( < 0.001), and BMI reduced from (30.14 ± 1.68) kg/m to (28.34 ± 2.33) kg/m ( < 0.001). Interaction analysis revealed significant synergistic effects between TECAR and HMB in improving the SPPB total scores ( = 16.374, < 0.001, ² = 0.107) and reducing BMI ( = 14.328, < 0.001, ² = 0.095). CONCLUSION: TECAR therapy combined with HMB supplementation significantly enhances physical function, activities of daily living, and body composition in elderly patients with sarcopenic obesity, demonstrating a synergistic effect.
[Risk Factors for Mortality in Hemodialysis Patients With Gastrointestinal Bleeding].
Cai T, Chen Y, Xing X … +2 more , Xiao X, Yang J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113428 · Full text
OBJECTIVE: The risk of death in patients undergoing maintenance hemodialysis (MHD) significantly increases if they develop concomitant gastrointestinal bleeding (GIB). This study aims to investigate the clinical characte...
OBJECTIVE: The risk of death in patients undergoing maintenance hemodialysis (MHD) significantly increases if they develop concomitant gastrointestinal bleeding (GIB). This study aims to investigate the clinical characteristics of MHD patients with concomitant GIB, identify risk factors associated with in-hospital mortality among them, and provide a basis for the early clinical identification and optimized clinical management of this specific patient population. METHODS: The clinical data of MHD patients with GIB admitted to West China Hospital, Sichuan University between July 2019 and May 2024 were collected and a retrospective analysis was conducted accordingly. The patients were divided into a death group and a survival group based on their discharge status. Clinical characteristics, laboratory test results, endoscopic findings, etc., of the two groups were collected. Oversampling was used to reduce the bias caused by data imbalance between the two groups, and stepwise logistic regression and other methods were used for analysis. RESULTS: A total of 212 patients were included, with 40 in the death group and 172 in the survival group. According to the findings of logistic regression, the following were identified as independent risk factors for mortality among the patients: activated partial thromboplastin time (APTT) (odds ratio [OR] = 1.014; 95% CI, 1.002-1.027; = 0.024), Glasgow-Blatchford bleeding score (GBS) (OR = 2.348; 95% CI, 1.686-3.269; < 0.001), and age-corrected Charlson comorbidity index (aCCI) (OR = 1.522; 95% CI, 1.185-1.954; < 0.001), and small intestinal vascular malformation (OR = 0.3722; 95% CI, 0.161-0.858; = 0.020). CONCLUSION: For MHD patients with concomitant GIB, APTT, GBS, aCCI, and small intestinal vascular malformation are independent risk factors for in-hospital death.
[Dynamic Succession of Urokinase-Type Plasminogen Activator in an Oral Squamous Cell Carcinoma Model].
Luo Y, Huang X, Cui B … +2 more , Ji N, Zhang P
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113427 · Full text
OBJECTIVE: To systematically characterizes the temporal changes in urokinase-type plasminogen activator (uPA) over the course of neoplastic progression using a mouse oral squamous cell carcinoma (OSCC) model induced by 4...
OBJECTIVE: To systematically characterizes the temporal changes in urokinase-type plasminogen activator (uPA) over the course of neoplastic progression using a mouse oral squamous cell carcinoma (OSCC) model induced by 4-nitroquinoline-1-oxide (4-NQO). METHODS: A total of 65 wild-type C57BL/6 mice of 5 weeks old were randomly assigned to two groups, a 4-NQO group ( = 50), which received daily administration of 100 μg/mL 4-NQO in drinking water, and a control group ( = 15), which received sterile water. At 12, 16, 20, 22, and 24 weeks, 10 mice from the 4-NQO group and 3 from the control group were randomly selected, weighed, and sacrificed. Tongue tissues were collected for hematoxylin-eosin (HE) staining to preliminarily assess OSCC development, and for immunofluorescence staining and quantitative real-time PCR to evaluate dynamic uPA expression in tongue tissues during OSCC progression. RESULTS: After 16 weeks of exposure, 4-NQO-treated mice exhibited significantly lower body mass compared with that of the controls ( < 0.05) and the weight loss became increasingly more pronounced over time. Histopathological changes in tongue tissues progressed in a clearly time-dependent manner-hyperplasia and mild dysplasia emerged at week 12, while moderate-to-severe dysplasia and carcinoma were observed by week 22, yielding a tumorigenic rate of 25%, which escalated to 70% by week 24. Immunofluorescence and qPCR analyses demonstrated a pronounced, progressive up-regulation of uPA expression in lesional tissues as OSCC progressed ( < 0.0001). CONCLUSION: This study not only confirmed the uniqueness of the 4-NQO model in OSCC research, but also revealed the changes in uPA during tumor invasion. These findings provide a theoretical foundation for the development of early diagnosis and precision treatment strategies, holding significant potential clinical value and research importance for improving patient prognosis.
[Mid-Term Efficacy Evaluation of Laparoscopic Sacrocolpopexy vs Laparoscopic Pectopexy for Pelvic Organ Prolapse].
Lei Y, Sun Y, Sun T … +3 more , Han X, Zhao Z, Miao Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113426 · Full text
OBJECTIVE: To evaluate the perioperative safety and mid-term outcomes of laparoscopic sacrocolpopexy (LSC) and laparoscopic pectopexy (LP) for pelvic organ prolapse (POP). METHODS: A retrospective analysis was conducted...
OBJECTIVE: To evaluate the perioperative safety and mid-term outcomes of laparoscopic sacrocolpopexy (LSC) and laparoscopic pectopexy (LP) for pelvic organ prolapse (POP). METHODS: A retrospective analysis was conducted on 274 POP patients, including 178 who underwent LSC and 96 who underwent LP, between August 2017 and January 2023. The extent of prolapse and anatomical restoration were assessed preoperatively and postoperatively using the Pelvic Organ Prolapse Quantification (POP-Q) system. Quality of life outcomes were evaluated with validated questionnaires, including Pelvic Floor Distress Inventory-short form 20 (PFDI-20), Pelvic Floor Impact Questionnaire-short form 7 (PFIQ-7), and Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire-12 (PISQ-12). Postoperative patient satisfaction was assessed during follow-ups. Postoperative anatomical restoration, perioperative status, and postoperative complications, recurrence, and quality of life were compared between the two groups. Multivariate logistic regression was performed to identify postoperative risk factors for recurrence. RESULTS: The operative time in the LSC group was significantly shorter than that in the LP group ( < 0.05). Intraoperative blood loss was higher in the LSC group compared to that in the LP group ( < 0.05). The LSC group also exhibited higher rates of de novo stress urinary incontinence and constipation ( < 0.05). The mean follow-up duration was (35.91 ± 16.90) months. The positions of the indicator points (Aa, Ba, C, Ap, and Bp) in the POP-Q classification after the operation were all better than those before the operation. The PFDI-20 score, PFIQ-7 score, and PISQ-12 score all improved compared to those before the operation ( < 0.05). Comparison of preoperative and postoperative PFDI-20, PFIQ-7, and PISQ-12 scores showed no intergroup differences. Compared with the LP group, the LSC group had the lower preoperative POP-Q measurements at points Aa and Ba ( < 0.05), but superior postoperative measurements for all the indicator points (Aa, Ba, C, Ap, and Bp) ( < 0.05). Recurrence occurred in 28 cases in the LP group and 4 cases in the LSC group, with the LP group presenting a significantly higher anatomical recurrence rate than the LSC group did (31.46% [28/89] vs. 2.41% [4/166], < 0.05). The subjective cure rate (100%) and objective cure rate (97.59%) in the LSC group were superior to those in the LP group (88.76% and 68.54%, respectively; < 0.05). The results of the multivariate logistic regression analysis showed that, after adjusting for the confounding factors, including age, gravidity, parity, body mass index, and duration of POP, the risk of recurrence after LSC surgery was 0.044 times that after LP (odds ratio [OR], 0.044; 95% CI, 0.015-0.133; < 0.001). CONCLUSION: Mid-term outcomes of LP with partial cervical preservation appear inferior to those of LSC, with LSC demonstrating superior anatomical restoration and lower rates of anatomical recurrence. However, improvements in sexual function and quality of life are comparable between the two procedures. Further evaluation with larger sample sizes and longer follow-up is warranted to better characterize long-term outcomes.
[Advances in Surgical Treatment Following Conversion Therapy for Pancreatic Cancer].
Meng L, Li Y, Peng B
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113425 · Full text
Pancreatic cancer is characterized by an insidious onset, high invasiveness, and poor response to treatment. Radical resection remains the only potentially curative approach currently available. However, approximately 80...
Pancreatic cancer is characterized by an insidious onset, high invasiveness, and poor response to treatment. Radical resection remains the only potentially curative approach currently available. However, approximately 80% of patients are already in the locally advanced or metastatic stage at initial diagnosis and have missed the opportunity for radical surgery. In recent years, with the development of novel drugs and updates to chemotherapy regimens, significant progress has been made in improving the efficacy of conversion therapy. Conversion therapy aims to transform initially unresectable tumors, such as locally advanced or metastatic pancreatic cancer, into a resectable state through systemic therapies (including chemotherapy, targeted therapy, immunotherapy, etc.) combined with localized treatments (such as radiotherapy, interventional therapy, etc.). This approach reduces tumor volume and eliminates micrometastases, thereby improving surgical resection rates and patient survival outcomes. However, considerable controversy remains regarding surgical treatment after conversion therapy for pancreatic cancer. This article provides an overview of conversion therapy regimens and treatment cycles, methods for evaluating therapeutic efficacy post-conversion therapy, the resection rates, and treatment outcomes of surgery following conversion therapy. The key technical points of post-conversion therapy surgery, including R0 resection, venous management, arterial resection and reconstruction, and the management of periarterial divestment, are highlighted. The learning curve for arterial resection and reconstruction is also discussed. Additionally, the potential applications of minimally invasive techniques (such as laparoscopy and robotics) are highlighted. The integration of biomarkers, imaging, and artificial intelligence holds promise for optimizing individualized decision-making. Multidisciplinary collaboration, the comprehensive competence of the surgical team, and the mastery and refinement of surgical skills are also critical factors that influence resectability and therapeutic outcomes after conversion therapy.
[Advances in the Structure and Function of Neurofilament Protein and Its Application in Early Diagnosis of Amyotrophic Lateral Sclerosis].
Maidina A, Wang F
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Jul · PMID 41113424 · Full text
Neurofilament proteins (NFs), defined as a type Ⅳ intermediate filaments, are important components of the neuronal cytoskeleton. They play a key physiological role in maintaining the structural integrity and plasticity o...
Neurofilament proteins (NFs), defined as a type Ⅳ intermediate filaments, are important components of the neuronal cytoskeleton. They play a key physiological role in maintaining the structural integrity and plasticity of axons and in ensuring the axonal transport function. Under pathological conditions, NFs detach from axons and undergo abnormal aggregation, causing axonal transport dysfunction. In addition, some components of the detached NFs leak into the peripheral circulation system. In patients with amyotrophic lateral sclerosis (ALS), the concentration of NFs is significantly elevated in the cerebrospinal fluid and blood, and the changes in NFs concentration is significantly positively correlated with the disease progression of ALS, suggesting the potential of NFs being used as early diagnostic biomarkers for ALS. In this review, we explored the relationship between NFs structure, assembly, and physiological function, focusing on the molecular mechanisms and clinical manifestations of ALS caused by abnormal assembly of NFs. We comprehensively summarized recent advances in the application of NFs as a new humoral biomarkers for early diagnosis and therapeutic monitoring of ALS. Key challenges in biomarker development-including undefined pathological neurofilament light chain (NFL) fragments, limited antibody availability, and poor assay reproducibility-are discussed. Strategies, including ultrasensitive detection technologies such as single molecule array (Simoa), antibody optimization based on pathological fragment identification, and multi-omics biomarker panels, should be integrated. These approaches may lead to breakthroughs, pave the way for precision-based ALS diagnosis, provide theoretical support for promoting its clinical translation and application, and offer ideas for future research.
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