The Kobe Journal Of Medical Sciences[JOURNAL]
Sun
200 papers
Investigation of the Treatment and Living Assistance Needed by Patients with Young-Onset Parkinson's Disease.
Iwasa Y, Saito I, Fujii C
Kobe J Med Sci · 2019 Feb · PMID 30988265
PURPOSE: This study assessed the symptoms, treatment, social systems use, and perception of living conditions of patients with young-onset Parkinson's disease (YOPD), and investigated the support needed by them. METHOD:...
PURPOSE: This study assessed the symptoms, treatment, social systems use, and perception of living conditions of patients with young-onset Parkinson's disease (YOPD), and investigated the support needed by them. METHOD: Among the 252 people who completed our questionnaire, we defined YOPD patients as those diagnosed as young onset or those with onset at ≤40 years. The data were compared with others. RESULTS: There were 24 patients with YOPD (9.5%) (average age: 61.7 years), with an average disease duration 6.4 years longer (p < 0.01) and time until diagnosis 0.7 years longer (p < 0.1) than those of other patients. This group took 1.6 times more types of medicines, and time to their next appointment was 8.6 days shorter than that of other patients (p < 0.05). Patients with YOPD had more pulsive walking and more sweating (p < 0.05), and more motor fluctuation (p < 0.1). More patients with YOPD had a physical disability certificate but felt they were not obtaining the required services (p < 0.05). 45.0% of the YOPD group wanted to work more, more used information and communication equipment (p < 0.05), and more felt their medications were adequate (p < 0.1). CONCLUSIONS: Increased awareness of YOPD is needed. YOPD patients have motor fluctuation because of the longer disease duration. Thus, the support of doctors and nurses, and frequent examination visits, are indispensable for controlling symptoms to achieve middle age developmental tasks. Increased support for care-giving, leisure-time activities, and work is also necessary and may help maintain the desire to work in this group.
Questionnaire Survey for Bronchial Asthma in Elderly Care Facilities.
Shinke H, Kamiryo H, Umezawa K … +7 more , Hori S, Nakata K, Nagano T, Hazeki N, Kobayashi K, Fukabori T, Nishimura Y
Kobe J Med Sci · 2019 Feb · PMID 30988264
In developed countries such as North America, the decline in mortality from bronchial asthma has ceased since 2006. The decline in mortality rate is also decreasing in Japan, where about 1,500 asthma deaths have been rep...
In developed countries such as North America, the decline in mortality from bronchial asthma has ceased since 2006. The decline in mortality rate is also decreasing in Japan, where about 1,500 asthma deaths have been reported. Among these, elderly people aged 65 years or over account for about 90% of cases. Therefore, the treatment of elderly patients with asthma is an important subject. However, few studies have been conducted on asthma in elderly patients. In this survey, we distributed a questionnaire to 253 elderly care facilities in Kobe, Japan. Ninety facilities responded, and 223 patients in 70 out of 90 facilities were diagnosed with asthma. Dry powder inhaler was the most commonly used dosage form of inhaled corticosteroids. Many facilities have patients who need some assistance during inhalation: only 60% of facilities reported that inhalation is performed accurately. While 31 facilities had patients with a history of hospitalization for asthma attacks, only 14 of these facilities were able to provide appropriate initial treatment. Many facilities have difficulty providing assistance with inhalation to elderly patients whose cognitive function has deteriorated. This survey highlights challenges experienced by care facilities in treating asthma in the elderly.
Emotional and Behavioral Problems of Pre-school Children with Autistic Spectrum Disorder Assessed by the Child Behavior Checklist 1½-5.
Irwanto, Kahfi M, Febriyana N … +2 more , Hartini S, Takada S
Kobe J Med Sci · 2019 Jan · PMID 30988263
BACKGROUND: Children with Autism Spectrum Disorder (ASD) experience a significant disruption in the ability to communicate and interact socially. Early detection is important; the earlier the child is identified with ASD...
BACKGROUND: Children with Autism Spectrum Disorder (ASD) experience a significant disruption in the ability to communicate and interact socially. Early detection is important; the earlier the child is identified with ASD, the faster the intervention can begin. The Child Behavior Checklist (CBCL) is a screening tool capable of identifying various emotional and behavioral disorders. Research data on the use of the CBCL as a screening tool for ASD are still limited in Indonesia. The present study aimed to evaluate the CBCL's ability to detect emotional and behavioral problems in pre-school children with ASD. METHODS: A cross sectional study was conducted in Surabaya, Indonesia. Children aged 1½-5 years old diagnosed with ASD, and typically developing (TD) children were enrolled using total sampling. The CBCL/1½-5 questionnaire for parents, consisting of 99 items, was used in this research to evaluate emotional and behavioral problems. Data were analyzed with the Independent-Sample T-test to compare the CBCL/1½-5 scale between ASD and TD children, while the proportion of gender was evaluated by a chi-square test with a 95% confidence interval. RESULTS: Fifty children were included in this research, 25 with ASD and 25 TD. Seventy six percent (38) were male and 24% (12) were female. Six of the seven scales were significantly higher in ASD children than TD in the CBCL/1½-5. Children with ASD had higher internalization (p < 0.0001), externalization (p = 0.001), and total problem scores (p < 0.0001) when compared to TD children. CONCLUSION: The CBCL/1½-5 can significantly detect the emotional and behavioral problems in pre-school children with ASD.
Mothers'Breastfeeding-Related Durations and Nursing Management During the Early Postpartum Period in a Mixed Hospital Ward with an Obstetrics Department: A Prospective Observational Study.
Mitsui Y, Saito I
Kobe J Med Sci · 2019 Jan · PMID 30988262
PURPOSE: This study aimed to quantify duration of in-hospital breastfeeding (BF)-related activities in mothers after childbirth in a mixed ward-type hospital with an obstetrics department in Japan (hereafter, mixed ward)...
PURPOSE: This study aimed to quantify duration of in-hospital breastfeeding (BF)-related activities in mothers after childbirth in a mixed ward-type hospital with an obstetrics department in Japan (hereafter, mixed ward). METHODS: Twenty-two postpartum mothers (primiparous and multiparous) who had vaginal delivery in a mixed ward were instructed to wear radio beacons. A smartphone was placed in the BF room and detected the presence of a nearby beacon, 24 hours daily, measuring the number of hours a mother spent in the nursing room (defined as BF time [hereafter, BF-related duration]). BF-related duration included time spent for BF, diaper changes before and after BF, bottle feeding, and support from nurses. BF-related total hours during postpartum hospitalization were calculated. The effects of parity and each postpartum day number (postpartum days 1-4) on Daily BF-related duration were determined. RESULTS: Percentage of total postpartum hospitalization time spent for BF-related duration was 21.6% and 19.9% for primiparous and multiparous mothers, respectively, with no significant difference between groups. BF-related duration was significantly different between parity group and postpartum day, with peaks on postpartum day 4 (387.7 minutes) and day 2 (318.0 minutes) for primiparous and multiparous groups, respectively. (F = 2.813, p = 0.048). CONCLUSION: Mothers spent 20% of their postpartum hospitalization period for BF-related activities. Individual support is necessary, especially for primiparous mothers, who spent more time than multiparous mothers for BF-related activities on postpartum day 4. In a mixed ward, postpartum mothers need a comfortable hospital environment in which midwives can dedicate themselves to mother-child dyad care.
Fetal Primary Small Bowel Volvulus Associated with Acute Gastric Dilatation Detected by Ultrasonography.
Fukushima S, Fujioka K, Ashina M … +10 more , Ohyama S, Ikuta T, Nishida K, Miyauchi H, Okata Y, Bitoh Y, Tanimura K, Deguchi M, Yamada H, Iijima K
Kobe J Med Sci · 2019 Jan · PMID 30728342
Fetal intestinal volvulus is a rare condition, and fetal diagnosis of this disease is still challenging, especially in primary cases not accompanied by other comorbidities, such as intestinal malformations. Herein, we re...
Fetal intestinal volvulus is a rare condition, and fetal diagnosis of this disease is still challenging, especially in primary cases not accompanied by other comorbidities, such as intestinal malformations. Herein, we report a case of fetal primary small bowel volvulus associated with acute gastric dilatation detected by ultrasonography. We speculate that the mechanism of acute gastric dilatation in our case was peristatic malfunction of the whole intestine caused by a strangulated ileus resulting from fetal intestinal volvulus. In conclusion, acute gastric dilatation detected by fetal ultrasound can indicate the fetal intestinal volvulus.
Morphometric Measurements of the Hip Bone in Turkish Adult Population.
Demir M, Atay E, Güneri B … +7 more , Yilmaz H, Arpaci MF, Güler HS, Al Ö, Ertekin T, Nisari M, Unur E
Kobe J Med Sci · 2018 Dec · PMID 30728341
OBJECTIVES: Coxal bone paticipates in the formation of the pelvic skeleton. Anatomy knowledge on coxafemoral joint as well as careful history taking and physical examination are crucial in evaluation and management of di...
OBJECTIVES: Coxal bone paticipates in the formation of the pelvic skeleton. Anatomy knowledge on coxafemoral joint as well as careful history taking and physical examination are crucial in evaluation and management of disorders involving hip joint. The aims of the present study were to perform morphometric measurements of the human coxal bones, calculation of their articular surface areas and report the range of these parameters regarding Turkish adult population. METHODS: Seventy-two dry human adult coxal bones (39 left and 33 right) from the Anatomy Departments of Erciyes University, Inonu University and Kahramanmaras Sutcu Imam University were measured using a caliper sensitive to 0.1 mm. Morphometric measurements were performed through 22 parameters determined. While 19 of these parameters were related to the distance between two points and thicknesses in various parts of the bone, the remaining three were related to the determination of articular surface areas. The articular surface areas of hip bone (facies auricularis (FA), facies lunata (FL) and facies symphsialis (FS)) were calculated with ImageJ software program. RESULTS: The average values of facies auricularis area were 1659.04 ± 470.92 mm² and 1637.32 ± 460.15 mm² on the left and right coxal bones, respectively. No statistically significant difference was determined between the left and right coxal bone measurements (p > 0.05). We found a positive and significant correlation between articular surface areas of facies auricularis (FA), facies lunata (FL) and facies symphysialis (FS) and maximum width of ilium (rFA = 0.299, rFL = 0.276, rFS = 0.375, respectively and p < 0.05), and distance between spina ilica anterior superior and the upper edge of facies symphysialis (rFA = 0.268, rFL = 0.511, rFS = 0.482, respectively and p < 0.05). CONCLUSION: The distribution and mean values of coxal bone morphometric measurements usually differ between individuals and human populations. With this regard, orthopedic surgeons should be aware of the diversity in components of coxal bone dimensions although implants and hip prosthesis components of different sizes are manufactured. Safe routes and estimated distances should be considered during surgical procedures to avoid complications.
Short-Term and Long-Term Outcomes of Infliximab and Tacrolimus Treatment for Moderate to Severe Ulcerative Colitis: Retrospective Observational Study.
Otsuka T, Ooi M, Tobimatsu K … +9 more , Wakahara C, Watanabe D, Adachi S, Yasutomi E, Yamairi H, Ku Y, Yoshida M, Hoshi N, Kodama Y
Kobe J Med Sci · 2018 Dec · PMID 30728340
BACKGROUND/AIMS: While some studies have shown that IFX and TAC exhibit similar efficacy against UC in the short-term, it is unclear which drug produces better long-term outcomes. In this study, we compared the long-term...
BACKGROUND/AIMS: While some studies have shown that IFX and TAC exhibit similar efficacy against UC in the short-term, it is unclear which drug produces better long-term outcomes. In this study, we compared the long-term efficacy of IFX and TAC in patients with moderate to severe UC. METHODS: This retrospective study was conducted from 2009 to 2017. It included patients with no history of IFX or TAC treatment. We analyzed the clinical response and remission rates at 12 and 52 weeks, and colectomy-free and relapse-free survival were evaluated until the end of the study. RESULTS: At 12 weeks, 94.4% and 77.8% of the patients in the IFX group (n = 18) had demonstrated clinical responses and clinical remission, respectively, whereas 72.7% of the patients in the TAC group (n = 11) exhibited clinical responses and clinical remission. The clinical response, clinical remission, and colectomy-free rates did not differ significantly between the groups. At 52 weeks, clinical responses and clinical remission had been achieved in 76.5% and 70.6% of the patients both in the IFX group, respectively. In the TAC group, clinical responses and clinical remission were achieved in 50.0% of patients. Relapse-free and colectomy-free survival were estimated significantly better in IFX group evaluated by Kaplan-Meier curves. CONCLUSION: This study indicates that IFX and TAC produce similar short-term outcomes in UC patients, but IFX produces better long-term outcomes than TAC especially with avoidance of colectomy. Our data suggest that IFX therapy may be prioritized over TAC for the treatment of moderate to severe UC.
An Open-Label, Multi-Institutional, Randomized Study to Evaluate the Additive Effect of a Leukotriene Receptor Antagonist on Cough Score in Patients with Cough-Variant Asthma Being Treated with Inhaled Corticosteroids.
Miwa N, Nagano T, Ohnishi H … +10 more , Nishiuma T, Takenaka K, Shirotani T, Nakajima T, Dokuni R, Kawa Y, Kobayashi K, Funada Y, Kotani Y, Nishimura Y
Kobe J Med Sci · 2018 Dec · PMID 30728339
Cough-variant asthma is one of the most common reasons for chronic cough. It is important to treat appropriately cough-variant asthma because 30% to 40% of cough-variant asthma becomes a typical asthma. However, little i...
Cough-variant asthma is one of the most common reasons for chronic cough. It is important to treat appropriately cough-variant asthma because 30% to 40% of cough-variant asthma becomes a typical asthma. However, little is known about the treatment of cough-variant asthma except for inhaled corticosteroid (ICS). The aim of this study was to validate the additive efficacy of a leukotriene receptor antagonist (LTRA) on cough score and respiratory function in patients with cough-variant asthma being treated with ICS. A total 28 patients were randomly assigned to either an ICS + LTRA group or an ICS group. There were statistically significant improvements in cough scores in the ICS + LTRA group from 0 weeks (6.7 ± 4.4) to 2 weeks (2.9 ± 3.2) (P < 0.05), 4 weeks (0.7 ± 1.1) (P < 0.001), and 8 weeks (0.8 ± 1.2) (P < 0.001). However similar improvements were not evident in the ICS group from 0 weeks (6.7 ± 4.4) to 2 weeks (5.6 ± 10.0) (P = 0.59), 4 weeks (4.6 ± 7.6) (P = 0.32), and 8 weeks (2.9 ± 5.2) (P = 0.08). On the other hand, no significant changes were evident in the forced expiratory volume in 1 s (FEV1) and FEV1/forced vital capacity (FVC). In conclusion, the LTRA was useful in improving cough in patients with cough-variant asthma, even though it appeared to be ineffective in improving respiratory function.
The Transition of Sleep Behaviors in Twin Infants and Their Mothers in Early Infancy.
Kondo C, Takada S
Kobe J Med Sci · 2018 Dec · PMID 30728338
BACKGROUND: The mothers of twins often suffer from sleeplessness. However, little is known about the relation of sleep behaviors between these mothers and their infants. The change of this relation with age has not been...
BACKGROUND: The mothers of twins often suffer from sleeplessness. However, little is known about the relation of sleep behaviors between these mothers and their infants. The change of this relation with age has not been reported. The aims of this study are firstly to clarify the sleep behaviors of twin infants and their mothers by using actigraphy (four measurement periods at approximately 4- to 6-week intervals) and secondary to evaluate the relations of sleep behaviors between twin infants and their mothers. METHODS: Five twin pairs and their mothers (first-time mother) were participated in this prospective longitudinal study. Their sleep behaviors were recorded for 7 consecutive days by using an actigraph, when the infants reached a corrected age (CA) of 3-6 weeks, 8-11 weeks, 13-15 weeks, and 17-20 weeks. Sleep status was classified into 3 states: both infants sleeping, only one infant sleeping, and both infants awake. RESULTS: All infants were cobedded. The time awake during the nocturnal period decreased by almost 90 minutes from CA 3-6 weeks to CA 8-11 weeks. Sleep duration in the nocturnal period increased by almost 85 minutes, and the proportion of time with both infants sleeping rapidly increased in the same period. Maternal sleep duration during the period of both infants sleeping was positively correlated with CA. CONCLUSION: This research revealed the transition of sleep behaviors in twin infants and their mothers in early infancy. Cobedding may facilitate more synchronized sleep states of twin infants.
Deletion Polymorphism of Angiotensin-Converting Enzyme Gene Is Associated with Low Muscle Mass in Elderly People in Jakarta, Indonesia.
Handayani MDN, Sadewa AH, Farmawati A … +1 more , Rochmah W
Kobe J Med Sci · 2018 Nov · PMID 30666042
Decline in muscle mass due to aging is a growing public health problem as it contributes to a decreased capacity for independent living among elderly people. A clear understanding of genetic factors is important, as it i...
Decline in muscle mass due to aging is a growing public health problem as it contributes to a decreased capacity for independent living among elderly people. A clear understanding of genetic factors is important, as it is known that angiotensin-converting enzyme insertion/deletion (ACE I/D) polymorphism affects muscle mass, although the findings are frequently heterogeneous. This study was conducted to determine the association between ACE I/D polymorphism and muscle mass in elderly people. A total of 130 elderly people were recruited from nursing homes in Jakarta. Anthropometric components affecting the muscle mass were examined. Cross-sectional analyses were performed to compare data using t-test, ANOVA and ANCOVA, and linear regression. Genotyping of the ACE I/D polymorphisms was performed by PCR methods, and muscle mass was evaluated by BIA. Genotype distribution counts II 65.38%, ID 13.85%, and DD 20.77% were not consistent with the Hardy-Weinberg equilibrium (χ² = 22.2, df = 2; p < 0.01). Individuals with the DD genotype showed lower muscle mass that was significantly different compared to the muscle mass in individuals with the II/ID genotype (II 16.14 ± 0.38, ID 15.71 ± 0.59; DD 13.95 ± 0.61 kg), after adjusting for % fat as a covariate. The linear regression analysis showed that age, gender, weight, height, nutritional status, protein content, and waist, hip, and calf circumference were significant contributors to muscle mass. In the multivariate analysis, adjusted age and gender significantly correlated with muscle mass, with r² = 0.98, by the likelihood ratio test (p < 0.01). The genotype variability accounted for 2.65% of the DD genotype. This study showed that in an elderly population in Jakarta, the DD genotype was associated with low muscle mass. This result suggests the role of nutritional status as a potential mediator in the association between ACE gene and muscle mass.
Case Series of Comminuted Olecranon Fracture Treated by Plate Fixation; Do We Have to Remove the Plate?
Inui A, Kuroda T, Kurosawa T … +4 more , Kokubu T, Mifune Y, Nishimoto H, Kuroda R
Kobe J Med Sci · 2018 Nov · PMID 30666041
Comminuted olecranon fracture requires surgical intervention. Plate fixation has been performed on the majority of cases. We reviewed the cases of comminuted olecranon fracture in young and middle age treated by plate os...
Comminuted olecranon fracture requires surgical intervention. Plate fixation has been performed on the majority of cases. We reviewed the cases of comminuted olecranon fracture in young and middle age treated by plate osteosynthesis and analyzed the functional outcome, complications and ratio and timing of hardware removal. Fifteen cases of comminuted olecranon fractures treated by plate fixation were reviewed. Bone union was achieved in all cases, the average range of motion at the final follow up was -11° in extension, 133° in flexion, 89° in pronation and 88° in supination. Hardware removal performed in 12 cases in average 8.3 months postoperatively, in 2 cases elbow joint contracture release was performed during the hardware removal. In the co-payment (+) group, 4 cases (67%) removed the plate at average six months postoperatively. On the other hand, no co-payment group (workman's compensation insurance or automobile liability insurance) underwent hardware removal surgery in 8 cases (89%) at 9.6 months postoperatively. There was no statistical difference between the timing or prevalence of hardware removal between the groups. The present study showed high removal rate of hardware despite the excellent clinical result. The surgeons should be aware that plate fixation of the olecranon fracture requires the removal of a plate in the majority of cases.
Reduction of Serum Uric Acid Associated with Attenuation of Renal Injury, Inflammation and Macrophages M1/M2 Ratio in Hyperuricemic Mice Model.
Haryono A, Nugrahaningsih DAA, Sari DCR … +2 more , Romi MM, Arfian N
Kobe J Med Sci · 2018 Oct · PMID 30666040
BACKGROUND: Hyperuricemia contributed to endothelial dysfunction, activation of the RAS system, increased oxidative stress and maladaptive immune system response. M1 and M2 macrophages were known to contribute to the ons...
BACKGROUND: Hyperuricemia contributed to endothelial dysfunction, activation of the RAS system, increased oxidative stress and maladaptive immune system response. M1 and M2 macrophages were known to contribute to the onset of renal fibrosis. This study aimed to look at the effect of lowering serum uric acid levels on renal injury in mice. METHODS: This study used 25 male mice, 3 months old, that divided into 5 groups. We injected uric acid intraperitoneally, 125mg/kg/day for 7 days (UA7) and 14 days (UA14), to induce hyperuricemia and then gave allopurinol 50mg/kg/day for 7 days to lower serum uric acid levels (UA7AL7 and UA14AL7). At the end of the treatment, we measured serum uric acid levels, Glomerular Injury Score (GIS) and Arteriolar Injury Score (AIS) with PAS staining, eNOS and MCP-1 expression with Reverse Transcriptase-PCR (RT-PCR), macrophages M1/M2 ratio with anti-CD68 and anti-arginase I immunohistochemical staining. Data were analyzed by one-way ANOVA and Kruskal-Wallis test. RESULTS: Uric acid injection increased serum uric acid levels in UA7 and UA14 group (p<0.05), followed by the increase in GIS and AIS. RT-PCR showed increased expression of MCP-1 and decreased expression of eNOS. M1 macrophages count was higher than control in UA7 and UA14 whereas M2 macrophages did not show any increased count, so the ratio of macrophages M1 / M2 is higher. Decrease in serum uric acid levels reduced GIS, AIS, MCP-1 expression and macrophages M1/M2 ratio (p<0.05). CONCLUSION: Reduction of serum uric acid levels significantly reduced renal injury that occurred in mice model of hyperuricemia.
Spatial Memory Disturbance Following Transient Brain Ischemia is Associated with Vascular Remodeling in Hippocampus.
Hermawati E, Arfian N, Mustofa … +1 more , Partadiredja G
Kobe J Med Sci · 2018 Oct · PMID 30666039
A number of studies have investigated the effects of ischemic injury on functional and cellular characteristics of hippocampus. There is only a limited study on vascular remodeling of it. The present study aimed at exami...
A number of studies have investigated the effects of ischemic injury on functional and cellular characteristics of hippocampus. There is only a limited study on vascular remodeling of it. The present study aimed at examining vascular remodeling in hippocampus and spatial memory disturbances after transient brain ischemia. Male Wistar rats were randomly divided into four groups, i.e. sham operated (SHAM), transient brain ischemia with 1 day reperfusion (IR1), 3 day reperfusion (IR3), and 10 days reperfusion (IR10) groups. Transient brain ischemia was induced by bilateral common carotid artery occlusion (BCCAO). The spatial memory test was performed using the Morris water maze (MWM) in SHAM and IR10 groups. The rats were euthanized at day 1, 3 or 10 after BCCAO depending on the groups. The mRNA expressions of SOD2, Bcl-2, NeuN, eNOS, endothelin-1 (ET-1), CD31, VE-cadherin and vascular remodeling of the hippocampus were examined. There were deteriorations of spatial learning ability in IR10 group. The percentages of SOD2 and Bcl-2, the expression of NeuN, decreased and the vascular remodeling was observed in the ischemic groups. The eNOS and CD31 expressions were less in IR10, the VE-cadherin expression was less in all ischemic groups than in SHAM group, while ET-1 expression in IR1 group was higher than any other groups. The spatial memory deterioration after BCCAO is associated with vascular remodeling in hippocampus, characterized by lumen narrowing and smooth muscle thickening of microvessels.
Potential of Coffee Fruit Extract and Quinic Acid on Adipogenesis and Lipolysis in 3T3-L1 Adipocytes.
Duangjai A, Nuengchamnong N, Suphrom N … +3 more , Trisat K, Limpeanchob N, Saokaew S
Kobe J Med Sci · 2018 Oct · PMID 30666038
This study was to assess the impact of different colors of coffee fruit (green, yellow and red) on adipogenesis and/or lipolysis using 3T3-L1 adipocytes. Characterization of chemical constituents in different colors of c...
This study was to assess the impact of different colors of coffee fruit (green, yellow and red) on adipogenesis and/or lipolysis using 3T3-L1 adipocytes. Characterization of chemical constituents in different colors of coffee fruit extracts was determined by ESI-Q-TOF-MS. The cytotoxicity of the extracts in 3T3-L1 preadipocytes were evaluated by MTT assay. Oil-red O staining and amount of glycerol released in 3T3-L1 adipocytes were measured for lipid accumulation and lipolysis activity. All coffee fruit extracts displayed similar chromatographic profiles by chlorogenic acid > caffeoylquinic acid > caffeic acid. Different colors of raw coffee fruit possessed inhibitory adipogenesis activity in 3T3-L1 adipocytes, especially CRD decreased lipid accumulation approximately 47%. Furthermore, all extracts except CYF and their major compounds (malic, quinic, and chlorogenic acid) increased glycerol release. Our data suggest that different colors of coffee fruit extract have possessed anti-adipogenic and lipolytic properties and may contribute to the anti-obesity effects.
Selective Inferior Mesenteric Artery Embolization during Endovascular Abdominal Aortic Aneurysm Repair to Prevent Type II Endoleak.
Fukuda T, Matsuda H, Tanaka H … +3 more , Sanda Y, Morita Y, Seike Y
Kobe J Med Sci · 2018 Jul · PMID 30617246
PURPOSE: The purpose of this study was to evaluate the efficacy of simultaneous IMA (s-IMA) embolization during the endovascular abdominal aortic aneurysm repair (EVAR). MATERIALS AND METHOD: From July 2007 to January 20...
PURPOSE: The purpose of this study was to evaluate the efficacy of simultaneous IMA (s-IMA) embolization during the endovascular abdominal aortic aneurysm repair (EVAR). MATERIALS AND METHOD: From July 2007 to January 2011, 189 patients in the no embolization (NE) group underwent EVAR without the indication for s-IMA embolization. Since February 2011 to April 2014, 143 patients have undergone EVAR. Among these patients, 26 patients underwent s-IMA embolism under a predefined indication and constituted the simultaneous embolization (SE) group. The indications for s-IMA embolization were defined by preoperative computed tomography (CT) findings, as follows: (1) the diameter was greater than 2.5 mm and (2) no stenosis due to thrombus or calcification at its orifice. RESULTS: The incidence of a type II endoleak from the IMA was 3.4% (5/143) in the SE group patients and 13.2% (25/189) in the NE group patients (p = 0.013), and the incidence of a type II endoleak from all branches (i.e., IMA, lumbar, medial sacral arteries) was 15.4% (22/143) in the SE group patients and 32.3% (61/189) in the NE group patients (p = 0.0003). During the follow-up period (range, 6-72 months; mean: 28 months), the reintervention rate for a type II endoleak from the IMA and/or other branches was 9.5% (18/189) in the NE group and 0.6% (1/143) in the SE group (p = 0.0001). CONCLUSION: In selected patients, performing an s-IMA embolization, based on CT findings, decreased the incidence of a type II endoleak and reintervention from the IMA and from all branches.
Anti-inflammatory Effects of Hibiscus Sabdariffa Linn. on the IL-1β/IL-1ra Ratio in Plasma and Hippocampus of Overtrained Rats and Correlation with Spatial Memory.
Bayani GFE, Marpaung NLE, Simorangkir DAS … +6 more , Sianipar IR, Ibrahim N, Kartinah NT, Mansur IG, Purba JS, Ilyas EII
Kobe J Med Sci · 2018 Oct · PMID 30381729
Overtraining leads to an increase in IL-1β systemically due to muscle microtrauma, which affects the hippocampus, an important structure in spatial memory consolidation. The administration of Hibiscus sabdariffa Linn is...
Overtraining leads to an increase in IL-1β systemically due to muscle microtrauma, which affects the hippocampus, an important structure in spatial memory consolidation. The administration of Hibiscus sabdariffa Linn is expected to decrease IL-1β and increase IL-1ra, thereby potentially preventing impairments in spatial memory consolidation. This research was an experimental study using 20 male Wistar rats. The overtraining of Wistar rats altered the ratio of IL-1β/IL-1ra in the plasma and hippocampus. Moreover, this overtraining impaired spatial memory consolidation. The methanol extract of H. sabdariffa improved spatial memory consolidation in Wistar rats and prevented impairment in spatial memory consolidation by maintaining the ratio of IL-1β/IL-1ra in the plasma and hippocampus of Wistar rats who experienced overtraining. H. sabdariffa is a potent anti-inflammatory substance that prevents impairments in spatial memory consolidation in overtrained Wistar rats.
Uncoupling Protein 2 (UCP2) as Genetic Risk Factor for Obesity in Indonesia is Different in Gender Stratification.
Surniyantoro HNE, Sadewa AH, Hastuti P
Kobe J Med Sci · 2018 Oct · PMID 30381728
Ala55Val and 45 basepair (bp) insertion/deletion (I/D) of UCP2 gene polymorphisms cause a decrease in resting energy expenditure, decreasing fatty acid oxidation and influencing mRNA transcription and stability, thereby...
Ala55Val and 45 basepair (bp) insertion/deletion (I/D) of UCP2 gene polymorphisms cause a decrease in resting energy expenditure, decreasing fatty acid oxidation and influencing mRNA transcription and stability, thereby increasing the risk of obesity. The purpose of this study was to determine the role of Ala55Val and 45 bp I/D polymorphisms of the UCP2 gene as a risk factor for obesity. This study consisted of 200 Indonesian subjects of Javanese ethnicity consisting of 100 obese and 100 non-obese participants. Examination of Ala55Val (C > T) UCP2 genotype used Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods and 45 bp I/D genotype used PCR methods. Polymorphism of Ala55Val UCP2 genotype in the male group showed that TT genotype and T allele significantly lowers the risk of obesity. Insertion/deletion of 45 bp UCP2 gene in the male group showed that II genotype and I allele significantly increase the risk of obesity whereas for women it showed that the DI genotype and I allele lower the risk of obesity. The results of this study demonstrate that Ala55Val and 45 bp I/D UCP2 polymorphisms play a role in the risk of obesity in Javanese ethnicity of Indonesia after gender stratification.
Impact of Cytochrome P450 2C19 Reduced-Function Polymorphism on Lesions and Clinical Outcome in Japanese Patients After Drug-eluting Stent Implantation.
Tahara N, Shinke T, Otake H … +3 more , Nishio R, Konishi A, Hirata KI
Kobe J Med Sci · 2018 Sep · PMID 30381727
We previously reported that the cytochrome P450 (CYP) 2C19 reduced-function polymorphism was associated with decreased responsiveness to clopidogrel and intra-stent thrombus formation, as well as subsequent ischemic even...
We previously reported that the cytochrome P450 (CYP) 2C19 reduced-function polymorphism was associated with decreased responsiveness to clopidogrel and intra-stent thrombus formation, as well as subsequent ischemic events after drug-eluting stent (DES) implantation. However, the relationship between the polymorphism and bleeding events remains unclear. Among 1427 consecutive patients who underwent DES implantation at Kobe University Hospital, 247 patients (341 lesions) were enrolled for this prospective observational study. All patients underwent follow-up optical coherence tomography (OCT) at 8 months and CYP2C19 genotyping. The patients were divided into three groups according to the phenotypic effect of the CYP2C19 polymorphism: extensive metabolizers (EM), intermediate metabolizers (IM), and poor metabolizers (PM). OCT findings, and ischemic and bleeding events were compared among the three groups. The frequency of intra-stent thrombi showed an increasing pattern among the patients with EM, IM, and PM (13.3%, 22.6%, and 33.3%, respectively; p = 0.04). The incidence of major adverse cardiovascular events (MACE) also showed an increase across the three groups from extensive to poor metabolizers (7.8%, 10.5%, and 33.3%, respectively; p < 0.01), whereas the frequency of bleeding showed no significant difference among the groups (15.6%, 19.4%, and 21.2%, respectively; p = 0.69). The CYP2C19 polymorphism is associated with the frequency of MACE, but is not related to the incidence of bleeding after percutaneous coronary intervention in Japanese patients receiving clopidogrel.
Docosahexaenoic Acid Reduces Palmitic Acid-Induced Endoplasmic Reticulum Stress in Pancreatic Β Cells.
Suzuki E, Matsuda T, Kawamoto T … +10 more , Takahashi H, Mieda Y, Matsuura Y, Takai T, Kanno A, Koyanagi-Kimura M, Asahara SI, Inoue H, Ogawa W, Kido Y
Kobe J Med Sci · 2018 Sep · PMID 30381726
Endoplasmic reticulum (ER) stress leads to peripheral insulin resistance and the progression of pancreatic beta cell failure in type 2 diabetes. Although ER stress plays an important role in the pathogenesis of diabetes,...
Endoplasmic reticulum (ER) stress leads to peripheral insulin resistance and the progression of pancreatic beta cell failure in type 2 diabetes. Although ER stress plays an important role in the pathogenesis of diabetes, it is indispensable for cellular activity. Therefore, when assessing the pathological significance of ER stress, it is important to monitor and quantify ER stress levels. Here, we have established a novel system to monitor ER stress levels quickly and sensitively, and using this method, we have clarified the effect of differences in glucose concentration and various fatty acids on the ER of pancreatic β cells. First, we developed a cell system that secretes Gaussia luciferase in culture medium depending on the activation of the GRP78 promoter. This system could sensitively monitor ER stress levels that could not be detected with real-time RT-PCR and immunoblotting. This system revealed that hyperglycemia does not induce unfolded protein response (UPR) in a short period of time in MIN6 cells, a mouse pancreatic β cell line. Physiological concentrations of palmitic acid, a saturated fatty acid, induced ER stress quickly, while physiological concentrations of oleic acid, an unsaturated fatty acid, did not. Docosahexaenoic acid, an n-3 unsaturated fatty acid, inhibited palmitic acid-induced ER stress. In this study, we have established a system that can sensitively detect ER stress levels of living cells in a short period of time. This system can be used to monitor the state of the ER in living cells and lead to the investigation of the significance of physiological or pathological ER stress levels.
Endometrial Stromal Sarcoma Arising from Endometrial Polyp: A Case Report.
Sato S, Ojima Y, Kanda M … +2 more , Kizaki T, Ohara N
Kobe J Med Sci · 2018 Sep · PMID 30381725
Endometrial stromal sarcoma (ESS) is a rare malignant tumor of the uterus. We report an uncommon case of ESS composed of both low-grade ESS and high-grade ESS arising from an endometrial polyp. On the findings of magneti...
Endometrial stromal sarcoma (ESS) is a rare malignant tumor of the uterus. We report an uncommon case of ESS composed of both low-grade ESS and high-grade ESS arising from an endometrial polyp. On the findings of magnetic resonance imaging and contrast computed tomography, a patient was suspected of having uterine malignant tumor. She underwent a total abdominal hysterectomy with bilateral salpingo-oophorectomy. Macroscopically, the tumor was a polypoid lesion in the uterine cavity. The tumor was an endometrial polyp with ESS components. ESS was composed of low-grade ESS and high-grade ESS. By immunohistochemistry, both an endometrial polyp and low-grade ESS showed a positivity for CD10, estrogen receptor (ER), and progesterone receptor (PR). However, high-grade ESS showed only a focal and weak CD10 positivity with no immunostaining for ER and PR. A focal or diffuse positivity for α-smooth muscle actin and desmin was noted in both low-grade and high-grade ESS. The positive rates of Ki-67 and p53 in high-grade ESS were elevated up to over 95%. She was diagnosed as having ESS in a stage IA. After surgery, she received no further treatment. She has been without recurrence for 4 years since an initial surgery. In conclusion, immunohistochemical analyses are useful for make an accurate diagnosis of ESS showing a transition from low-grade ESS to high-grade ESS in addition to the conventional method.
About
- Frequency
- Sun
- Papers found
- 200
- RSS feed
- Subscribe