We developed and evaluated a new method of titanium surface treatment for direct bone fixation of implants. This method consists of hydroxyapatite (HA) flame coating onto a porous titanium surface which is arc-sprayed wi...We developed and evaluated a new method of titanium surface treatment for direct bone fixation of implants. This method consists of hydroxyapatite (HA) flame coating onto a porous titanium surface which is arc-sprayed with pure titanium material in argon gas at atmospheric pressure. The surface roughness of the porous layer was 360 μm, Rmax, and showed higher resistance to blast erosion in comparison with the conventional low-pressure plasma-sprayed porous layers of titanium. The thickness of the HA layer was between 20 and 40 μm considering the balance of bone conduction effect of HA and the possibility of mechanical detachment of the layers from the porous titanium. Short-term animal tests showed excellent results. This new surface treatment method was applied to cementless total hip joints which were used clinically. The results obtained from observations of the retrieved specimens show that the thickness of the HA coating layer is sufficient for the new bone formation after implantation. It was concluded that the new surface treatment method for titanium alloy implants is effective and successful for direct bone fixation.
The role of complete dentures in masticatory function is well established, but their effect on swallowing has been little examined. This study investigated the effect of complete dentures on swallowing in the elderly. La...The role of complete dentures in masticatory function is well established, but their effect on swallowing has been little examined. This study investigated the effect of complete dentures on swallowing in the elderly. Lateral projection videofluorography (VF) was performed for nine healthy edentulous elderly volunteers (mean age 73.9 +/- 2.9 years) while they swallowed 8 ml of liquid and paste barium with and without complete dentures. A total of 35 VF records were analyzed for temporal and spatial measurements of oropharyngeal motion during swallowing. The tongue tip contacted the inside of the lip between the upper and lower alveolar ridges during swallowing without but not with complete dentures. The hyoid moved further upward (P = 0.002) during swallowing without dentures (liquid: 5.85 +/- 3.99 mm, paste: 6.41 +/- 5.25 mm) than with dentures (liquid: 3.70 +/- 4.57 mm, paste: 3.69 +/- 4.07 mm). The larynx moved further forward (P = 0.044) during swallowing without dentures (liquid: 6.39 +/- 2.58 mm, paste: 6.46 +/- 2.87 mm) than with dentures (liquid: 5.34 +/- 2.04 mm, paste: 5.93 +/- 1.50 mm). The timing of the movements of these structures was not affected by the presence of the dentures or bolus consistency. These results showed that the hyoid and larynx moved more in swallowing without than with complete dentures, perhaps because of tongue tip protrusion when not wearing dentures. These findings suggest that complete dentures have a significant effect on swallowing function as well as masticatory function in elderly individuals.
This study evaluated the effect of titration in oral appliance therapy for obstructive sleep apnea syndrome (OSAS), and examined problems with this test. However, the method of predicting the appropriate mandible positio...This study evaluated the effect of titration in oral appliance therapy for obstructive sleep apnea syndrome (OSAS), and examined problems with this test. However, the method of predicting the appropriate mandible position has not yet been established. In this study, titration was attempted in order to predict appropriate mandible position prior to wearing an oral appliance. Twenty-three male patients diagnosed as OSAS by a physician participated in this study. The mandible was protruded by a titratable splint (TS) until apneic and hypopneic signs had disappeared. Moreover, polisomnography (PSG) was used to monitor brain wave patterns, eye movement, muscle tone, body movement and breathing. Sleep study was performed by a portable sleep monitoring device before and after examination wearing titrated oral appliance (OA), and the effect of therapy was evaluated. The results obtained were as follows. 1. In the Apnea Hypopnea Index (AHI), Apnea Hypopnea density (AH density), lowest SpO2, Oxygen Desaturation Index (ODI), there was a statistically significant improvement. The mean AHI reduced from 13.8 to 4.7 (p<0.001). The mean AH density reduced from 12.0 to 3.5 (p<0.001). The mean lowest SpO2 increased from 78.7 to 84.7 (p<0.0001). The mean ODI reduced from 15.7 to 6.1 (p<0.001). 2.. The average proportion of protrusive distance for movable distance was 71.7%. 3. Only one patient complained of discomfort in the maxillofacial region, however, this discomfort disappeared after adjustment of OA. Therefore, it is suggested that titration for OA is a very useful examination for OSAS therapy.
The purpose of this research was to clarify the structural changes according to the aging of the buccal mucous membrane in the elderly. Thirty-eight Japanese adult cadavers (62-98 years) which dental students had used fo...The purpose of this research was to clarify the structural changes according to the aging of the buccal mucous membrane in the elderly. Thirty-eight Japanese adult cadavers (62-98 years) which dental students had used for anatomy practice were used as materials. Serial sections were made from the specimens of the buccal mucous membrane in the vicinity of angulus oris removed from the cadavers, and the sections were observed with a light microscope. The results were as follows: 1) As for the buccal mucous membrane in the vicinity of angulus oris in the elderly, the thickness decreases significantly with aging. 2) In the buccal mucous membranes of the elderly, the functional arrangement of the collagenous and elastic fibers distributed in the lamina propria and the submucous membrane disappears with aging, and the fibrosis tendency generally becomes remarkable. 3) The number of blood vessels distributed in the mucous membrane in the vicinity of angulus oris of the elderly decreases with aging, and the distribution of blood vessels changes, too. It seems that these changes suppress the circulatory system of the buccal mucous membrane in the very elderly. 4) The fatty change and the fibrosis of the small salivary gland progress among the very elderly. It seems that the structural change of such a glandular tissue causes dryness in the mouth. 5) The change in the thickness of the tunica muscularis according to aging was hardly seen among the elderly. However, the tendency toward thinness with aging was seen, as for the muscular fiber. The structural change of such a muscular fiber seems to cause depression such as that of the orbicular muscle of the mouth in the elderly.
This study assessed the thresholds of orofacial touch and pressure sensation in children and compared them with those in adults. Child subjects who were outpatients of TMDU Dental Hospital and who had no particular syste...This study assessed the thresholds of orofacial touch and pressure sensation in children and compared them with those in adults. Child subjects who were outpatients of TMDU Dental Hospital and who had no particular systemic diseases other than dental problems, were classified into 4 groups: preschool children (4-5 y, n=26), lower-grade elementary school pupils (6-9 y, n=100), higher-grade elementary school pupils (10-12 y, n=36), and junior high school pupils (13-15 y, n=26). Adult subjects were TMDU students (23-31 y, n=37). The thresholds of touch and pressure sensation were measured with a Semmes-Weinstein pressure aesthesiometer which consisted of 20 filaments with different marking values (Fm). Stimulation was applied with the thinner 10 filaments with lower values ranging from 1.65 to 4.31 Fm (0.0045-2.052 gw). The measurements were carried out on the following 10 points in the orofacial area in all the subjects: the upper lip, gingiva of upper anterior teeth, palate, lower lip, gingiva of lower anterior teeth, buccal mucous membrane, tongue tip, skin above eyebrow, cheek skin and mentum skin. The thresholds in the child subjects were not different from those in the adult subjects on most measurement points, but were significantly different on the gingiva and palate. The lower the age, the lower the thresholds on the gingiva. Since these measurement points are located in the chewing mucous membrane, it is considered that the threshold changes occurred due to histological alterations with growth. It is suggested that the threshold values of orofacial touch and pressure sensation obtained from this study could be used as the standard values for an indicator of the development of oral function and structure in children.
The aim of this study was to identify genes responsible for the formation and maintenance of cartilage tissue. For this purpose, gene expression patterns were compared between differentiating and dedifferentiating chondr...The aim of this study was to identify genes responsible for the formation and maintenance of cartilage tissue. For this purpose, gene expression patterns were compared between differentiating and dedifferentiating chondrocytes by cDNA macroarray analysis. A total of 1,186 genes were examined, and at least 7 genes were identified as candidate genes having critical roles in cartilages. Three of them encode well-known cartilage matrix molecules: aggrecan, decorin and type IX collagen, and others were preproenkephalin A, Early Growth Response-1, Early Growth Response-3, and basigin. All these genes were expressed at high level in mature cartilage, but their expressions were suppressed in response to dedifferentiation of chondrocytes. Meanwhile, transcription factor ALY, variant in the kinase (vik), and syndecan 2 genes were newly activated during the process of chondrocyte dedifferentiation. A similar process is somewhat observed in osteoarthritis. Therefore, activation of these genes might be closely related to the progression of osteoarthritis and would be a useful marker for it.
To fabricate a prosthesis which is functionally harmonized with the stomatognathic system, the interocclusal relationship must be reproduced as accurately as possible. This study investigated the influence of the occlusa...To fabricate a prosthesis which is functionally harmonized with the stomatognathic system, the interocclusal relationship must be reproduced as accurately as possible. This study investigated the influence of the occlusal contacts upon tooth displacement and clarified the optimal occlusal contacts for the prosthesis. The displacement paths of six maxillary left first molars of six adults in good general health were measured in this study. We gave each test tooth eight patterns of artificial occlusal contacts by attaching platinum foil on the occlusal surface. Subjects were asked to perform clenching at the intercuspal position (= ICP) with medium occlusal forces, and 3-dimensional tooth displacement paths were measured by the Type M-3 tooth displacement transducer. The following conclusions were obtained. 1. Using the Student's t-test for statistical analysis, it was suggested that the difference of the buccolingual location of occlusal contacts influenced the direction of the tooth displacement. 2. The tooth which had occlusal contact in the buccal cusp displaced in the mesio-buccal direction, so this may create a wide dental arch and a slight discrepancy was generated at the distal interproximal contact area of the tooth. 3. It was shown that the tooth which had occlusal contact in the buccal cusp rotated in the buccoapical direction in reverse to the natural tooth.
The aim of this study was to define transcriptional changes that occur during dexamethasone-induced in vitro osteoblastic differentiation using human mesenchymal stem cells (hMSCs). Bone marrow derived hMSCs from three i...The aim of this study was to define transcriptional changes that occur during dexamethasone-induced in vitro osteoblastic differentiation using human mesenchymal stem cells (hMSCs). Bone marrow derived hMSCs from three individual donors were grown in DMEM containing 10% fetal bovine serum and antibiotics (basal media, BM). At confluence (Day 0), cells were grown in BM or BM plus ascorbic acid, beta-glycerophosphate and dexamethasone (OS). At 0, 3, 7 and 14 days, total RNA was isolated from hMSCs, and 32ρ-labelled probes were synthesized. Atlas 1.2 gene arrays (Clontech) were hybridized with donor-specific cDNA probes. Acquired data were analyzed using GeneSpring Software (Silicon Genetics) enabling normalization and averaging of donor-specific data sets at each time point. Only 8, 31, and 27 genes were upregulated more than 3-fold at 3, 7 and 14 days, respectively. Over 50 genes were downregulated at each time point. Interestingly, the results of this study did not reflect commonly identified genes that are frequently considered in cellular differentiation along the osteoblastic lineage. Instead, some interesting genes were upregulated. Seven days exposure to OS media resulted in the relatively high induction of several genes, including growth factors and receptors which affect adipocyte, chondrocyte and osteoblast differentiation. IGF II and FGFR 3, FGFR 2 were upregulated in the middle of 14 days' induction. Examination of gene expression at day 14 also revealed a number of receptors (LEPR) and transcription factors (ROR alpha) were upregulated during 14 days. These could be of importance to the process of early stage differentiation.
Bone changes its shape adaptively according to the mechanical environment, suggesting a close relationship between the bone remodeling and the stress induced in bones. We measured the geometries of the canine radius afte...Bone changes its shape adaptively according to the mechanical environment, suggesting a close relationship between the bone remodeling and the stress induced in bones. We measured the geometries of the canine radius after partial resection of the ulna with X-ray CT and microscopic study with fluorescent labeling. The experimental animals were six beagle dogs aged three months. The midshaft of their right ulna was partially resected. The experimental period of them was 6, 12 and 24 weeks. Sectional images of their antebrachium were taken every 2 or 4 weeks with X-ray CT. The bone was labeled with Calcein and Xylenol Orange each week. From CT observation, significant bone growth was observed at cranial-lateral, caudal-medial side and resected ulnar side at 6 weeks after surgery. At 12 weeks, the thickness of cortical bone was increased. At 24 weeks, the cross-section changed to an oval or round shape. From confocal laser microscope observation, significant new bone formation was identified under the periosteum at the cranial-lateral and resected ulnar side from 2 weeks after surgery. On the control side, lamellar bone formation took place in an orderly manner, whereas on the experimental side, there was a large quantity of rapid woven bone formation filling the cavities of the resected ulna, followed by the lamellar bone formation. The bone formations took place to compensate for the mechanical functions of the lost ulna, suggesting the adaptive nature of bone remodeling.
A simple, broadly applicable, standardized dysphagia severity scale would be useful to standardize dysphagia evaluations, to monitor recovery and efficacy of treatment and to study the consequences of dysphagia. We devel...A simple, broadly applicable, standardized dysphagia severity scale would be useful to standardize dysphagia evaluations, to monitor recovery and efficacy of treatment and to study the consequences of dysphagia. We developed a global Dysphagia Severity Scale (DSS) from videofluorographic swallowing studies which included subjective clinical ratings of functional swallowing. We rated laryngeal penetration/aspiration (P/A) and pharyngeal retention (PR), and then assessed methods for scoring severity of P/A and PR and for combining them in a global scale. Each method was tested by correlation with overall clinical severity ratings. The highest correlations were found by: 1) scoring P/A based on which foods were aspirated (no aspiration or penetration; penetration only; thin liquid aspiration; thick liquid, pudding, or chewed solid food aspiration; 2) scoring PR based on how much food was retained (none; minimal; moderate; severe); and 3) combining P/A and PR by taking the higher of the two scores as the final DSS rating. The final global DSS score and the clinical severity rating correlated well (r = 0.71). This proposed scale shows promise for rating global dysphagia severity.
In 2002, the Dental Hospital of Tokyo Medical and Dental University set up a working group for risk management. This working group analyzed 225 incident and accident reports submitted to the hospital in 2001 and 2002. Ea...In 2002, the Dental Hospital of Tokyo Medical and Dental University set up a working group for risk management. This working group analyzed 225 incident and accident reports submitted to the hospital in 2001 and 2002. Each report was analyzed with regard to "type," "place," "reporter," "severity," and "cause" in order to diagnose hospital safety and prevent future incidents and accidents. The cause of incidents and accidents was analyzed using the SHEL model, where S stands for Software, H for Hardware, E for Environment, and L for Liveware. The severity of the consequence was classified into 6 levels, where level 0 = "error not applied," level 1 = "not affected," level 2 = "watch and see or additional test," level 3 = "treatment," level 4 = "aftereffect," and level 5 = "death." The incidents and accidents judged to have potentially high risk were given a score of "+H," irrespective of the level. The results of the analyses revealed that most of the incidents and accidents happened in "wards," "operation rooms," and "oral surgery clinics." This is probably because the incident and accident reporting system is well established by nurses working in these clinics. Additional analysis revealed that most of the reports were written and submitted by nurses. The frequencies of "treatment procedure," "misuse of dental instruments," "mis-prescription," "falling down" and "needlestick" related incidents and accidents were the highest and were caused mainly by L and S. There were only 3 accidents above level 4, however, less severe cases were given a score of +H due to the high potential risk involved.
This study examined the optimum occlusal contacts of implant prostheses to maintain good oral condition after treatment. Three subjects who had two contiguously missing teeth (first and second molars) were selected. The...This study examined the optimum occlusal contacts of implant prostheses to maintain good oral condition after treatment. Three subjects who had two contiguously missing teeth (first and second molars) were selected. The displacement path of the implant, the adjacent tooth and the antagonistic tooth during clenching were measured using the type M-3 three-dimensional tooth displacement transducer. The occlusal contacts of implant prostheses changed according to the following four conditions. A contact: the inner inclination of the upper buccal cusp, B contact: the inner inclination of the upper lingual cusp, C contact: the outer inclination of the upper lingual cusp, and ABC contact: including the above three contacts. The measurements were performed at least six months after implant surgery. In each subject, the implants and the adjacent teeth were not affected by the change of the occlusal contact of the implant prosthesis, but the opposing teeth were affected. In the case of implantation in the lower side, the opposing tooth displaced in the buccal direction with A contact and C contact, which was a different direction to that of the natural tooth. The antagonist with B contact displaced in the lingual direction, which was the same direction as that of the natural tooth. The antagonist with ABC contact displaced in the lingual or buccal direction. It is concluded that the occlusal adjustment of implants needs much care: in the case of only A contact or C contact, non-physiological distortion might occur in periodontal tissues of the opposing teeth of the implant.
We have carried out temporary silicone implants after diskectomies or arthroplasties in temporomandibular joint surgeries to avoid postoperative adhesion and to maintain articular space. We evaluated 19 joints in 15 pati...We have carried out temporary silicone implants after diskectomies or arthroplasties in temporomandibular joint surgeries to avoid postoperative adhesion and to maintain articular space. We evaluated 19 joints in 15 patients who had received dacron-reinforced silicone implant after silicone sheet removal through follow-up for at least 6 months. The cases included temporomandibular joint disorder (10 joints in 9 patients), psoriatic arthritis (2 joints in 1 patient), ankylosis (4 joints in 3 patients) and synovial chondromatosis (2 joints in 2 patients). On the basis of the criteria of temporomandibular dysfunction for the results, they were classified as bad (4 patients). It is thought that factors other than the implant are related to the bad results in the postoperative evaluation. In this study, lymphadenopathy induced by exfoliated silicone debris could not be confirmed. The temporary silicone implant in the temporomandibular joint was thought to be useful.
The clinical analysis of 462 patients (154 men and 308 women) with problems of implant therapy done at other clinics is reported. They visited the clinic for oral implant of the Tokyo Medical and Dental University Hospit...The clinical analysis of 462 patients (154 men and 308 women) with problems of implant therapy done at other clinics is reported. They visited the clinic for oral implant of the Tokyo Medical and Dental University Hospital during the period from January 1996 to March 2002. The results were as follows: 1) The majority of the patients (76.6%) were 40 to 69 years of age. 2) Of the total patients, 26.8% had been referred from the other outpatient clinics in our hospital, 14.7% from private dental offices and 2.6% from attending doctors. 3) There were 361 patients with complications related to the implant therapy. Seventy-six patients asked for second opinions concerned about a diagnosis or treatment recommendation. Thirty patients required maintenance of the implants. 4) The most common complications observed were peri-implantitis (184 cases). Prosthodontic complications (72 cases) included framework/resin/veneering material fractures, screw loosening and fractures. There were implant fractures in 21 cases, sensory disturbance in 20 cases, problems related to sinus in 13 cases, problems of connected teeth in 30 cases and phonetic/esthetic complications in 10 cases. 5) The types of problem implants were osseointegrated implants (196 cases), alumina-ceramic implants (74 cases), metallic blade-type implants (35 cases), pin-type implants (10 cases), subperiosteal implants (6 cases) and other implants.
This study compared the adaptation of a conventional and an electroformed porcelain-fused-to-metal crown. A master model was selected from an ITI implant with a solid abutment (height: 4 mm). Conventional cast metal fram...This study compared the adaptation of a conventional and an electroformed porcelain-fused-to-metal crown. A master model was selected from an ITI implant with a solid abutment (height: 4 mm). Conventional cast metal frameworks of 0.7 mm thickness were prepared with a high noble metal alloy (Degudent U, Degussa) for porcelain fusing (n = 5). Electroformed frameworks of 0.2 mm thickness were determined using pure gold deposition on the abutment using the Auro-Galva-Crown system (AGC, Wieland) (n = 5). Subsequently, a porcelain (Super Porcelain AAA, Noritake) was fused to each framework. Internal gaps between the framework and its abutment were determined using the thickness of a silicone fit checking material. The gaps were measured both before and after porcelain fusing. The thicknesses of the silicone layer of the electroformed and the conventional porcelain-fused-to-metal crown were 34.6 and 38.5 microns at the margin, 33.2 and 39.6 microns at the internal slope, 22.0 and 33.0 microns at the axial, 58.6 and 65.1 microns at the occlusal, respectively. Three-way analysis of variance revealed that the mean gaps in the electroformed porcelain-fused-to-metal crown were significantly thinner than those in the conventional porcelain-fused-to-metal crown (p < 0.05). The electroformed porcelain-fused-to-metal crown showed better adaptability than the conventional porcelain-fused-to-metal crown regardless of porcelain fusing.
This survey classified the issues involved in promoting the regular check-up system in dental clinics. The survey was conducted in two parts: interviews with 39 dental clinics and a questionnaire survey of 5,129 patients...This survey classified the issues involved in promoting the regular check-up system in dental clinics. The survey was conducted in two parts: interviews with 39 dental clinics and a questionnaire survey of 5,129 patients (response rate: 56.8%). The results are summarized as follows: 1. Among the dental clinics that participated in this survey, the proportion of regular patients, the number of dental hygienists working at clinics, and the number of patients per day were higher than those of the national average. 2. The demographic patterns of regularly attending patients were "female, aged under 14 years and students or public servants". 3. Satisfaction with the technical competence of hygienists has the strongest correlation with regularity of visiting. The degree of correlation was about twice that of the correlation with technical competence of the dentist. 4. Patients' priorities for improving dental services to enhance satisfaction were treatment charges, appointment system, and attitude of staff and doctors toward welcoming the patients.