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Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal Of Applied Physiology[JOURNAL]
Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal Of Applied Physiology[JOURNAL]
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200 papers
A Comprehensive Review on Ulcer and Their Treatment.
RaviKKumar VR, Rathi S, Singh S … +4 more , Patel B, Singh S, Chaturvedi K, Sharma B
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2023 Dec · PMID 38755116 · Publisher ↗
A peptic ulcer is a lesion (sore) on the stomach lining, or duodenum. Peptic ulcers are probably a twentieth-century condition. The ulcer disease continues to be a significant source of worldwide morbidity and mortality....
A peptic ulcer is a lesion (sore) on the stomach lining, or duodenum. Peptic ulcers are probably a twentieth-century condition. The ulcer disease continues to be a significant source of worldwide morbidity and mortality. The Gastrointestinal ulcers and duodenal ulcers are considered the two most extreme types of peptic ulcers. Peptic ulcers are found to be caused by an excess of violent factors including Hydrochloric acid (HCL) pepsin, refluxed bile leukotrienes (LT), reactive oxygen species (ROS) and protective factors, these include mucus-bicarbonate barrier functions, prostaglandins (PGs), mucosal blood flow, cell regeneration and migration, non-enzymatic and enzymatic and certain growth factors. The primary cause of peptic ulcer disease is pylori infection and the use of NSAIDs. This review article underscores the importance of a multidisciplinary approach in the management of ulcers to improve patient outcomes and quality of life.
Mucoadhesive Drug Delivery System and There Future Prospective: Are a Promising Approach for Effective Treatment?
Singh S, Chaurasia A, Rajput DS … +1 more , Gupta N
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2023 Dec · PMID 38751344 · Publisher ↗
Mucoadhesive polymers are a new and exciting development in drug delivery systems that have the potential to significantly increase therapeutic efficacy. These polymers stick to mucosal surfaces, increasing the amount of...
Mucoadhesive polymers are a new and exciting development in drug delivery systems that have the potential to significantly increase therapeutic efficacy. These polymers stick to mucosal surfaces, increasing the amount of time that medications stay at the site of absorption and improving their bioavailability. These mechanisms include longer contact times with the mucosal surface, better drug solubility, and defence against enzymatic degradation of pharmaceuticals. Mucoadhesive polymers also provide a number of benefits over traditional drug delivery methods, including less frequent dosage, better patient compliance, and fewer adverse effects. Due to their adaptability, Mucoadhesive polymers may be used in the rectal, vaginal, ophthalmic, nasal, and oral routes of drug delivery. Mucoadhesive polymers have advantages now, but they also have potential for the future of medication delivery. Mucoadhesion offers excellent possibilities for the delivery of a range of substances through the nasal, vaginal, buccal, and ocular routes of administration. Furthermore, mucoadhesion facilitates the achievement of an extended local or systemic pharmacological effect. In this study, we covered the mechanisms behind mucoadhesion, possible uses for Mucoadhesive polymers in drug administration, and techniques for assessing Mucoadhesive drug delivery systems. The goal of current research is to create innovative Mucoadhesive polymers that have better biodegradability, biocompatibility, and adhesive qualities. Moreover, it is anticipated that the effectiveness of Mucoadhesive polymers would be increased when combined with other cutting-edge drug delivery technologies, such as micro particles and nanoparticles.
Exploring Novel Herbal Compounds and Formulations for Inflammatory Bowel Disease (IBD) Management.
Dubey RK, Shukla S
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2023 Dec · PMID 38716509 · Publisher ↗
Inflammatory Bowel Disease (IBD), encompassing Crohn's disease and ulcerative colitis, presents a complex and challenging clinical scenario characterized by chronic inflammation of the gastrointestinal tract. Traditional...
Inflammatory Bowel Disease (IBD), encompassing Crohn's disease and ulcerative colitis, presents a complex and challenging clinical scenario characterized by chronic inflammation of the gastrointestinal tract. Traditional herbal medicine has garnered increasing interest as a potential adjunctive or alternative therapy for IBD, owing to its perceived efficacy, safety profile, and holistic approach to health. This review provides a comprehensive overview of the current landscape of herbal interventions for IBD, addressing scientific, regulatory, clinical, and patient-related considerations. Scientifically, the exploration of herbal interventions faces challenges related to the complexity of herbal formulations, standardization, and quality control. Regulatory hurdles encompass stringent requirements for safety, efficacy, and quality standards, necessitating adherence to robust preclinical and clinical protocols. Clinically, the heterogeneity of the patient population, potential interactions with conventional therapies, and patient preferences pose challenges in the integration of herbal interventions into clinical practice.
A Systematic Review of the Pharmacological and Phytochemical Profiles of Madagascar periwinkle as Potential Dietary Supplement.
Dubey RK, Shukla S, Hussain Z … +1 more , Tasin M
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2023 Dec · PMID 38651238 · Publisher ↗
Madagascar periwinkle (Catharanthus roseus) is a plant species known for its rich pharmacological and phytochemical properties. This systematic review aims to comprehensively evaluate the potential of Madagascar periwink...
Madagascar periwinkle (Catharanthus roseus) is a plant species known for its rich pharmacological and phytochemical properties. This systematic review aims to comprehensively evaluate the potential of Madagascar periwinkle as a dietary supplement. A thorough search of relevant databases yielded studies focusing on the pharmacological activities and phytochemical constituents of Madagascar periwinkle. The review highlights the diverse pharmacological effects of Madagascar periwinkle, including anti-cancer, anti-diabetic, anti-inflammatory, and antimicrobial properties, among others. Furthermore, the phytochemical analysis revealed the presence of various bioactive compounds such as alkaloids, flavonoids, terpenoids, and phenolics, which contribute to its medicinal properties. Despite the promising findings, further research is warranted to elucidate the mechanisms of action, safety profile, and potential interactions of Madagascar periwinkle as a dietary supplement. Overall, this systematic review provides valuable insights into the pharmacological and phytochemical profiles of Madagascar periwinkle, suggesting its potential as a natural dietary supplement with diverse health benefits.
An updated review on Emerging recent advances and biomedical application of silver nanocluster.
Kshatriya VV, Kumbhare MR, Jadhav SV … +2 more , Thorat PJ, Bhambarge RG
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2023 Dec · PMID 38651237 · Publisher ↗
Silver nanoclusters (AgNCs) have emerged as versatile nanomaterials with immense potential in theranostic applications, combining therapeutic and diagnostic functions in a single platform. This review provides a comprehe...
Silver nanoclusters (AgNCs) have emerged as versatile nanomaterials with immense potential in theranostic applications, combining therapeutic and diagnostic functions in a single platform. This review provides a comprehensive overview of recent advancements in the synthesis, characterization, and utilization of AgNCs for theranostics. The synthesis of AgNCs has witnessed significant progress, with numerous strategies such as chemical reduction, green synthesis, and templated approaches being employed to control size, shape, and stability. Their unique optical properties, including strong fluorescence and surface-enhanced Raman scattering (SERS) signals, make AgNCs ideal candidates for bioimaging and diagnostic purposes. Additionally, the surface chemistry of AgNCs allows for facile functionalization with targeting ligands and therapeutic agents, enhancing their specificity and efficacy. In the realm of diagnostics, AgNCs have been employed for various imaging modalities, including fluorescence imaging, photoacoustic imaging, and SERS-based sensing. Their excellent photostability and biocompatibility make them suitable for in vitro and in vivo imaging applications, enabling the real-time monitoring of disease progression and treatment response.
[Establishment of primary liver cancer model in mice].
Wang JJ, Li XY, Yi JK … +3 more , Zhao BL, Huang HM, Wei Y
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308442 · Publisher ↗
Three modeling methods were used to establish a mouse primary liver cancer model, and compared them to find a more optimal modeling method. Forty 15-day-old C3H/HeN male mice were randomly divided into groups I-IV, 10 m...
Three modeling methods were used to establish a mouse primary liver cancer model, and compared them to find a more optimal modeling method. Forty 15-day-old C3H/HeN male mice were randomly divided into groups I-IV, 10 mice in each group. Group Ⅰ were not treated; Group Ⅱ were intraperitoneally injected with 25 mg/kg diethylnitrosamine (DEN) once; Group Ⅲ were intraperitoneally injected with 100 mg/kg DEN once; Group Ⅳ were intraperitoneally injected with 25 mg/kg DEN once and followed by another intraperitoneal injection of 100 mg/kg DEN at 42 days of age. The mortality of mice in each group was analyzed. At the 18th week of modeling, blood was collected from eyeballs after anesthesia, and liver was taken from abdominal cavity after neck was broken. The appearance of liver, the number of cancer nodules and the incidence of liver tumor were observed. The histopathological changes of liver were observed by HE staining. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected. At the 18th week of modeling, compared with the group I, serum levels of ALT and AST in groups II-IV were increased significantly (＜0.05); The number of cancer nodules and the incidence of tumors in the surviving mice of groups III and IV were also increased significantly (＜0.05). At the 18th week of modeling, no mice died in both groups I and II, and the incidence of liver cancer was 0%; The incidence of liver cancer in surviving mice in both groups III and IV was 100%, but the mortality rate of mice in group III was as high as 50%, and that in group IV was only 20%. C3H/HeN male mice can successfully establish a mouse liver cancer model by intraperitoneal injection of 25 mg/kg of DEN once at the age of 15 days and another intraperitoneal injection of 100 mg/kg of DEN once at the age of 42 days with short cycle and low mortality, which is an ideal method to establish a primary liver cancer model.
[Effects of CUMS on excitatory/inhibitory balance of hippocampal and prefrontal cortex pyramidal neurons in anxiety-like mice].
Zhu CA, Chen JD, Yang HY … +2 more , Xia YP, Huang ZY
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308441 · Publisher ↗
To explore the changes in the excitatory/inhibitory (E/I) balance of pyramidal neurons in prefrontal cortex and hippocampus in mice with anxiety disorder induced by chronic unpredictable mild stress (CUMS). Twenty-four...
To explore the changes in the excitatory/inhibitory (E/I) balance of pyramidal neurons in prefrontal cortex and hippocampus in mice with anxiety disorder induced by chronic unpredictable mild stress (CUMS). Twenty-four C57/BL6 male mice were randomly divided into control group (CTRL) and model group (CUMS), with 12 mice in each group. The mice in CUMS group were subjected to 21 days of stress, including restraint for 1 h, reversed day/night cycle for 24 h, forced warm water bath for 5 min, water/food deprivation for 24 h, housing in wet sawdust for 18 h, shaking the cage for 30 min, noise for 1 h, and social stress for 10 min. CTRL group mice were fed normally. Anxiety-related behavioral tests and whole-cell recording tests were performed after modeling. Compared with CTRL group, the time of spent in the central arena of CUMS group was reduced significantly in open field test (＜0.01), the time and number of entering the open arms were decreased significantly in elevated plus maze test (＜0.01), and the time of staying in the closed arms was increased significantly in CUMS group (＜0.01). The sEPSC frequency, capacitance and E/I ratio of dlPFC, mPFC and vCA1 pyramidal neurons of mice in CUMS group were increased significantly (＜0.01), while sEPSC amplitude, sIPSC frequency, amplitude and capacitance were not significantly changed (＞0.05). The frequency, amplitude, capacitance and E/I ratio of sEPSC and sIPSC of dCA1 pyramidal neurons were not significantly changed (＞0.05). The anxiety-like behavior of CUMS-induced mice may be the result of the participation of multiple brain regions, which is mainly related to the increase of the excitability of pyramidal neurons in dlPFC, mPFC and vCA1 brain regions, but seems to have little relationship with dCA1 brain regions.
[Effects of repeated sevoflurane exposure on hippocampal cell apoptosis and long-term learning and memory ability in neonatal rats].
Bi XB, Zhang X, Wen JP … +2 more , Ding W, Li J
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308440 · Publisher ↗
To investigate the effects of repeated sevoflurane exposure on hippocampal cell apoptosis and long-term learning and memory ability in neonatal rats and its regulation on PI3K/AKT pathway. Ninety SD rats were randomly d...
To investigate the effects of repeated sevoflurane exposure on hippocampal cell apoptosis and long-term learning and memory ability in neonatal rats and its regulation on PI3K/AKT pathway. Ninety SD rats were randomly divided into the control group (25% oxygen), the single exposure group (inhalation of 3% sevoflurane and 25% oxygen on the 6th day after birth), the 3-times exposure group (inhalation of 3% sevoflurane and 25% oxygen on the 6th, 7th, and 8th day after birth), the 5-times exposure group (inhalation of 3% sevoflurane and 25% oxygen on the 6th, 7th, 8th, 9th and 10th day after birth), and the 5-times exposure + 740Y-P (PI3K activator) group (intraperitoneal injection of 0.02 mg/kg 740Y-P after inhalation of sevoflurane for 5 times) according to the random number table method. Morris water maze was used to measure the learning and memory ability; HE staining and transmission electron microscopy were used to observe the morphological and structural changes of neurons in the hippocampus; TUNEL was used to detect the apoptosis of hippocampal nerve cells; Western blot was used to detect the expressions of apoptosis-related proteins (Caspase-3, Bax, Bcl-2) and PI3K/AKT pathway related proteins in the hippocampus of rats. Compared with the control group and the single exposure group, the learning and memory abilities of rats in the 3-times exposure group and the 5-times exposure group were severely reduced, the morphology and structure of hippocampal neurons were severely damaged, and the apoptosis rate of hippocampal nerve cells was increased (＜0.05), the expressions of Capase-3 and Bax proteins were significantly increased (＜0.05), and the expressions of Bcl-2 protein and PI3K/AKT pathway protein were significantly decreased (＜0.05). With the increase in the number of exposures to sevoflurane, the learning and memory abilities of rats were significantly reduced, the hippocampal neuron cells were severely damaged, the hippocampal neuronal apoptosis rate was significantly increased (＜0.05), and the expressions of PI3K/AKT pathway proteins were significantly reduced (＜0.05). Compared with the 5-times exposure group, the learning and memory abilities and hippocampal neuron structure of rats in the 5-times exposure +740Y-P group were restored to a certain extent, and the hippocampal neuronal apoptosis rate, the levels of capase-3 and Bax protein were significantly reduced (＜0.05), while the expressions of Bcl-2 protein and PI3K/AKT pathway protein were increased significantly (＜0.05). Repeated exposure to sevoflurane can significantly reduce the learning and memory abilities of neonatal rats and exacerbate hippocampal neuronal apoptosis, which may be mediated by inhibiting the PI3K/AKT pathway.
[Effects of Bosutinib on cerebral ischemia/reperfusion injury in rats].
Zhang Y, Wu C, Zhang Q … +4 more , Kong Y, Bian XQ, Wang Y, Li S
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308439 · Publisher ↗
To investigate the effects of bosutinib on the early stage of cerebral ischemia-reperfusion injury in rats. Forty Sprague-Dawley rats were randomly divided into four groups (random number method), 10 rats in each group;...
To investigate the effects of bosutinib on the early stage of cerebral ischemia-reperfusion injury in rats. Forty Sprague-Dawley rats were randomly divided into four groups (random number method), 10 rats in each group; sham group (control group): only neck vessels were isolated without other treatments; MCAO (model group): the rat brain ischemia/reperfusion injury model was made by a modified wire bolus method,ischemia for 2 h followed by reperfusion for 24 h; DMSO group (solvent group): DMSO ( 0.752 ml/kg) was injected into the tail vein one day before the experiment, brain ischemia 2 h reperfusion for 24 h; Bosutinib group (intervention group): one day before the experiment, the tail vein was injected with Bosutinib (4 mg/kg), brain ischemia 2 h reperfusion for 24 h. After 24 h of ischemia reperfusion, neurological function score was performed; brain infarct area was calculated after staining with TTC; SIK2 was detected by Western blot; the contents of TNF-α and IL-6 in brain tissue were detected by ELISA. Compared with the sham group, the neurological function scores, the infarct volume percentages and the levels of inflammatory factors IL-6 and TNF-α of the MCAO and DMSO groups were increased significantly (＜0.05 or ＜0.01). Compared with the MCAO and DMSO groups, the above mentioned indexes of the bosutinib group were all decreased significantly (＜0.05 or ＜ 0.01). Compared with sham group, the expression levels of SIK2 protein in MCAO and DMSO groups had no significant changes(＞ 0.05); compared with the MCAO and DMSO group, the expression level of SIK2 protein in the bosutinib group was decreased significantly (＜0.05). Bosutinib reduces cerebral ischemia-reperfusion-induced injury, and its possible mechanism is related to the decreased expression of SIK2 protein and inflammatory factors.
[Effects of total saponins from Trillium tschonoskii Maxim on vascular cognitive impairment and its mechanisms].
Yang LJ, Wang G, Yang D … +3 more , Duan RZ, Zhao FY, Chen XB
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308438 · Publisher ↗
To investigate neuroprotective effects of total saponins from Trillium tschonoskii Maxim (TST) on vascular cognitive impairment (VCI) rats through inflammatory body of the NOD-like body protein 3 (NLRP3) regulated by end...
To investigate neuroprotective effects of total saponins from Trillium tschonoskii Maxim (TST) on vascular cognitive impairment (VCI) rats through inflammatory body of the NOD-like body protein 3 (NLRP3) regulated by endoplasmic reticulum stress (ERS). SD rats were divided into sham-operated group (SHAM), model group (VCI, bilateral neck arterial ligation (BCCAO) method), TST intervention group (TST, 100 mg/kg), and positive group (donepezil hydrochloride, 0.45 mg/kg ), continuous administration for 4 weeks. The ability of learning and memory was evaluated by the morris water labor. The tissue pathological changes were observed by HE and NISSL staining. Western blot was used to detectendoplasmic reticulum-related proteins GRP78, IRE1, XBP1. Inflammasome-related proteins NLRP3, ASC, Caspase-1, IL-18, IL-1β. Compared with the SHAM group, the escape latency of VCI group rats was prolonged significantly, and the number of times of crossing the platform and the percentage of target quadrant residence time were shortened (＜0.01); The cells in the hippocampus of VCI rats were damaged, with obvious pyknosis, decreased number of neurons and damage of cell body structure; The endoplasmic reticulum and inflammatory corpuscle-associated proteins were increased in VCI group (＜0.05 or ＜0.01). Compared with the VCI group, the TST group and the positive group had less time to search for the platform, and the ratio of the times of crossing the platform to the time in the target quadrant was longer (＜0.05 or ＜0.01). There was no significant difference in the times of crossing the platform between the positive group and VCI group (＞0.05); The cell damage, nuclear pyknosis and the number of neurons in TST and positive groups were significantly reduced; The endoplasmic reticulum associated proteins and inflammatory body associated proteins in TST group and positive group were decreased to different degrees (＜0.05 or ＜0.01). TST has neuroprotective effects on VCI rats, and its mechanism may be related to the involvement of ERS in the regulation of NLRP3 inflammatory small bodies.
[Influence of marine main engine operation on characteristics of VOCs in the ambient air of the ship main cabins].
Shi Y, Yu T, Shi HQ … +4 more , Leng WJ, Chen LF, Xi ZG, Lin BC
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308437 · Publisher ↗
Abstract loading — click title to view on PubMed.
[Alleviating effects of hydrogen on hyperhomocysteinemia and fatty liver induced by high-methionine diet].
Chu WB, Ding TQ, Wen B … +3 more , Lu JY, Fan R, Chen XW
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308436 · Publisher ↗
To investigate the alleviating effect of hydrogen (H) on homocysteine (Hcy) levels and non alcoholic fatty liver in rats with hyperhomocysteinemia (HHcy). After one week of adaptive feeding, Wistar rats were randomly di...
To investigate the alleviating effect of hydrogen (H) on homocysteine (Hcy) levels and non alcoholic fatty liver in rats with hyperhomocysteinemia (HHcy). After one week of adaptive feeding, Wistar rats were randomly divided into three groups: the general diet group (CHOW), the high methionine group (HMD), and the high methionine plus hydrogen rich water group (HMD+HRW), with 8 rats in each group. The CHOW group was fed with AIN-93G feed, while the HMD and HMD+HRW groups were fed with AIN-93G+2% methionine feed to construct an HHcy model. The HMD+HRW group was also gavaged with hydrogen rich water (3 ml/animal, twice a day, with a hydrogen concentration of 0.8 mmol/L), and body weight data were recorded. After 6 weeks of feeding, the plasma and liver samples were processed and collected. The plasma homocysteine (Hcy) and lipid contents of each group were measured, and the histological morphology of the liver was observed. The activities of key enzymes in the Hcy metabolism pathway and mRNA expression were detected in the liver. Compared with the CHOW group rats, the Hcy level in the blood of HMD rats was significantly increased significantly (＜0.05). Pathological tissue sections showed liver enlargement, injury, and fatty liver in the rats; Compared with the HMD group rats, the HMD+HRW group rats showed a significant decrease in Hcy in the blood, reduced liver damage, and increased Hcy metabolism key enzyme activity and mRNA expression in the liver, with statistical differences (＜0.05). Hydrogen has a significant improvement effect on liver injury induced by HMD diet in HHcy rats, possibly by enhancing the three metabolic pathways of Hcy to reduce excessive Hcy in the body, thereby improving liver metabolic function and symptoms of non-alcoholic fatty liver disease.
[Effects of curcumin on liver injury induced by chronic alcohol addiction].
Wu YM, Lin X, Su YJ … +2 more , Xue D, Zhang C
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308435 · Publisher ↗
To investigate the intervention effects of curcumin (Curc) on liver injury induced by chronic alcohol addiction in mice. Thirty Balb/c mice were randomly divided into normal control group (Control), model group (Model),...
To investigate the intervention effects of curcumin (Curc) on liver injury induced by chronic alcohol addiction in mice. Thirty Balb/c mice were randomly divided into normal control group (Control), model group (Model), low-dose Curc group (5 mg/kg, Curc-L), medium dose Curc group (10 mg/kg, Curc-M) and high-dose Curc group (15 mg/kg, Curc-H), with 6 mice in each group. The chronic alcohol addiction liver injury model was prepared with 20% liquor. The mice in control group were given 2 ml of normal saline every day. The mice in model group were given 5 ml/kg of 20% liquor every day, and the mice in Curc treatment group were treated with Curc at the doses of 5, 10, 15 mg/kg in 2 ml saline every day for 35 days. The weight of liver was measured and the health status of mice was observed. Serum ALT, AST, ALP and liver TG, TC, HDL-C, LDL-C, MDA, SOD, GSH-Px and NO were measured. The pathological changes of liver tissues stained with hematoxylin and eosin were observed. Compared with the control group, the liver mass and serum levels of ALT, AST, ALP, MDA, NO, TC, TG, HDL-C, LDL-C in the model group were increased significantly (＜0.05, ＜0.01), the activities of SOD and GSH-Px were decreased significantly (＜0.05, ＜0.01), the liver cells were vacuolated and infiltrated with inflammatory cells, and the expression levels of NF-κB and MAPK protein in liver tissues were increased significantly (＜0.01). Compared with the model group, the levels of ALT, AST, ALP, MDA, NO, TC, TG, HDL-C, LDL-C in Curc group were decreased significantly nd the activities of SOD and GSH-Px were increased significantly (＜0.05, ＜0.01). Curc can effectively reduce liver tissue damage by regulating NF-κB/MAPK signal pathway.
[Effects of Mijiandao suppository on intestinal laxation in rats and its mechanisms].
DU XF, Chen SF, Shang T … +3 more , Ni ZT, Shi YP, Wang JH
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308434 · Publisher ↗
To investigate the effects of Mijian Daotong Bowel Suppository (MJDs) on the compound diphenoxylate induced constipation model of male rats and its mechanisms. Sixty SD male rats were randomly divided into blank group,...
To investigate the effects of Mijian Daotong Bowel Suppository (MJDs) on the compound diphenoxylate induced constipation model of male rats and its mechanisms. Sixty SD male rats were randomly divided into blank group, model group, positive group and MJDs group. The constipation model was established by using compound diphenoxylate gavage. The rats in blank group and model group were treated with saline by enema, the rats in positive group and MJDs group were given Kaisailu and honey decoction laxative suppository by enema, respectively, once a day for 10 days. The body weight, fecal water content, gastric emptying rate (GER) and carbon ink propulsion rate (CIPR) of rats were observed during modeling and administration. The effects of MJDs on the pathological changes of colon tissue in constipation rats were investigated by hematoxylin-eosin (HE) staining. The effect of MJDs on 5-hydroxytryptamine (5-HT) in the colon of constipation rats was investigated by ELISA kit. The effects of MJDs on the expressions of aquaporins 3 (AQP3) and aquaporins 4 (AQP4) in the colon of constipation rats were detected by immunohistochemistry. After 10 days of administration, compared with the blank group, the body weight, fecal water content, carbon ink propulsion rate and colon 5-HT content in the model group were decreased significantly, while the expression levels of AQP3 and AQP4 in the colon were increased significantly (＜0.05, ＜0.01). Compared with the model group, the fecal water content and colon 5-HT content in the positive group were increased significantly, and the expressions of AQP3 and AQP4 in the colon were decreased significantly. The body weight, fecal water content and colon 5-HT content in the MJDs group were increased significantly, and the expressions of AQP3 and AQP4 was decreased significantly (＜0.05, ＜0.01). Compared with the positive group, the fecal water content of the MJDs group was decreased significantly, and the expressions of AQP3 and AQP4 in the colon of the MJDs group was decreased significantly (＜0.05, ＜0.01). Gastric emptying rate was not statistically significant difference between the groups. MJDs has good therapeutic effects on constipation, and its mechanisms may be related to up-regulating the content of 5-HT in the colon and down-regulating the expressions of AQP3 and AQP4 in the colon.
[Effects of Danzhen headache capsule on the expression of CREB and ERK in CM rats by mediated central sensitization mechanism].
Wang RQ, Ning YM, Wu GT … +2 more , DU LD, Wang ZW
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308433 · Publisher ↗
Abstract loading — click title to view on PubMed.
[Regulative effect of active components of Cistanche deserticola on intestinal dysbacteriosis induced by antibiotics in mice].
Han TY, Yang D, Zhou SQ … +4 more , Qiao YM, Yin J, Jin M, Li JW
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308432 · Publisher ↗
To study the effects of Cistanche deserticola and its active components Cistanche deserticola polysaccharide and Echinacoside on intestinal flora of antibiotic-associated diarrhea (AAD) mice. Forty-eight Balb/c mice wer...
To study the effects of Cistanche deserticola and its active components Cistanche deserticola polysaccharide and Echinacoside on intestinal flora of antibiotic-associated diarrhea (AAD) mice. Forty-eight Balb/c mice were randomly divided into control (Con) group, AAD Group, inulin (Inu) group, Cistanche deserticola (RCR) group, Cistanche deserticola polysaccharide (RCRDT) group and Echinacoside (Ech) group with 8 mice in each group. The diarrhea model of mice was induced by intragastric administration of lincomycin hydrochloride(3 g/kg) for 7 days, and then treated by intragastric administration of INU(5 g/kg), RCR(5 g/kg), RCRDT(200 mg/kg) and ECH (60 mg/kg),0.2 ml once a day for 7 days, Con group and AAD group were given the same volume of normal saline. By observing general signs of mice, colon HE staining, 16S rDNA high-throughput sequencing analysis, the effects of Cistanche deserticola, Cistanche deserticola polysaccharide and Echinacea glycoside on the imbalance of intestinal flora induced by antibiotics in mice were evaluated. Compared with Con group, AAD group mice lost weight, presented obvious diarrhea symptoms, inflammatory changes in colon tissue and decreased intestinal flora diversity (＜0.05) indicating the success of the model. Compared with AAD group, the weight and diarrhea of INU, RCR, RCRDT and ECH groups were significantly improved, and the colon pathology of ECH group was restored to normal level. Compared with AAD group, RCR group, RCRDT group and ECH group had significantly decreased intestinal increased and and decreased (＜0.05) . In ECH group, the abundance and diversity of intestinal microflora were returned to normal level, and the structure of intestinal microflora was well adjusted, the contents of and were increased (＜0.01). Both Cistanche deserticola and its active components cistanche deserticola polysaccharide and echinacoside can regulate the intestinal flora imbalance caused by antibiotics and improve the symptoms of AAD, especially echinacoside.
[Effects of nanopolystyrene nanoplastic exposure on the development and neurotoxicity of fetal rats during gestation].
Zhang YP, Tian L, Xie XQ … +3 more , Wang YT, Lyu P, Xi ZG
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308431 · Publisher ↗
To investigate the effects of polystyrene nanoplastics (PS-NPs) exposure during gestation on the growth and neurotoxicity of fetal rats. Twenty-seven SD pregnant rats were randomly divided into 9 groups with three rats...
To investigate the effects of polystyrene nanoplastics (PS-NPs) exposure during gestation on the growth and neurotoxicity of fetal rats. Twenty-seven SD pregnant rats were randomly divided into 9 groups with three rats in each group. The experimental group of PS-NPs was given 0.5, 2.5, 10 and 50 mg/kg of PS-NPs suspension with different particle sizes (25 and 50 nm) by gavage, wihe the control group was given ultrapure water by gavage. The time of gavage is from the 1st to the 18th day of pregnancy. The morphological changes of the placenta were observed; compare the number of male and female fetuses, live/dead/absorbed fetuses, body weight, body length, placental weight, and organ coefficients of kidney, liver, brain and intestine of fetal rats; the prefrontal cortex, hippocampus and striatum of the fetal rats were taken to measure related biochemical indicators. Compared with the control group, the placenta of the PS-NPs exposed group was found to have structural damage, which increased in a dose-dependent manner. The area ratio of trophoblast was significantly increased (＜0.05), and the area ratio of labyrinth was significantly decreased (＜0.05); In the prefrontal cortex, hippocampus and striatum of fetal rats, the levels of IL-1β, IL -6 and TNF-α were significantly increased in the 10 and 50 mg/kg PS-NPs exposed group (＜0.05), and more significantly elevated in the 25 nm group than those in the 50 nm group at 10 mg/kg exposure (＜0.05) the CAT activity was significantly decreased in 2.5, 10 and 50 mg/kg PS-NPs exposure groups (＜0.05), while the SOD and GSH-Px activities were significantly decreased in 25 nm exposure groups and 2.5, 10 and 50 mg/kg 50 nm PS-NPs exposure groups (＜0.05), the MDA content was significantly increased in 10, 50 mg/kg 25 nm PS-NPs exposure groups and 50 mg/kg 50 nm PS-NPs exposure groups (＜0.05). Maternal PS-NPs exposure during gestation may affect the growth and development of fetal rats by damaging the placental barrier and produce neurotoxicity in fetal rats, causing oxidative stress and inflammatory responses in various brain regions, and smaller particle sizes and higher doses of polystyrene nanoplastic exposure have more significant neurotoxic effects on the offspring.
[Effects of propranolol on biological function of human esophageal squamous cell carcinoma cells].
Zhuo QY, Qian H, Zhao BS … +2 more , Qi B, Liu YZ
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308430 · Publisher ↗
To investigate the effects of propranolol on the subcutaneous tumorigenesis of esophageal squamous cell carcinoma (ESCC) cells and the proliferation, migration, cell cycle, apoptosis and autophagy of ESCC cells and its p...
To investigate the effects of propranolol on the subcutaneous tumorigenesis of esophageal squamous cell carcinoma (ESCC) cells and the proliferation, migration, cell cycle, apoptosis and autophagy of ESCC cells and its possible molecular mechanisms. The cell proliferation was detected by MTT (methyl thiazol tetrazolium) assay: ESCC Eca109, KYSE-450 and TE-1 cells were routinely cultured. PBS (Phosphate buffer saline) group (without propranolol) and treated groups (40, 60, 80, 100 μmol/L propranolol) were set up with 5 wells in each group. After treatment for 0, 24, 48, 72 h, 10 μl (5 mg/ml) of MTT was added to each well, and the absorbance was measured at 490 nm. The cell migration was tested by Transwell assay: ESCC Eca109, KYSE-450 and TE-1 cells were routinely cultured, and PBS group (without propranolol) and treated groups (40, 60 μmol/L) were set up with 2 wells in each group. Photos were taken 40 h later, and the experiment was repeated for three times before statistical analysis. The cell cycle and apoptosis were detected by flow cytometry assay: ESCC Eca109, KYSE-450 and TE-1 cells were routinely cultured. PBS group (without propranolol) and treated group (80 μmol/L) were set up, fixed, stained, and fluorescence at 488 nm was detected. The protein levels were detected by Western blot: ESCC Eca109 and KYSE-450 cells were routinely cultured. PBS group (without propranolol) and treated groups (60, 80 μmol/L) were set up followed by gel electrophoresis, wet membrane transfer, and ECL imaging. The experiment was repeated for three times and then analyzed statistically. Subcutaneous tumor formation experiment in nude mice: 10 nude mice were assigned PBS group (without propranolol) and treated group (with propranolol). Five mice in each group were inoculated with 5×10 cells/100 μl (Eca109) into the right underarm. The treated group was given a gavage of 0.4 ml/kg (6 mg/kg) every other day, and the tumor size was measured every other day for 3 weeks. After 20 days, the nude mice were dislocated and sacrificed to take tumor tissue. The results showed that propranolol inhibited the proliferation of Eca109, KYSE-450 and TE-1 cells with IC of around 70 μmol/L for 48 h. Eca109, KYSE-450 and TE-1 cell migration was inhibited by propranolol in a dose-dependent manner (＜0.05); Propranolol blocked the cell cycle of Eca109 in G2/M phase, blocked the cell cycle of KYSE-450 and TE-1 in G0/G1 phase, and promoted apoptosis of three kinds of cells (＜0.05). The results of cell fluorescence showed that LC3 fluorescence intensity of TE-1 was increased after 12 h, 24 h and 36 h treatment with propranolol (＜0.05). Western blot results showed that compared with PBS group, the protein expressions of p-mTOR, p-Akt and cyclin D1 were down-regulated, while cleaved caspase 9 level was up-regulated (＜0.05). The results of subcutaneous tumor formation in nude mice showed that the tumor weight of PBS group was (0.91±0.05)g, and that of the experimental group was(0.65±0.12)g, the difference was statistically significant (＜0.05). Propranolol inhibits the proliferation, migration and cell cycle,promotes apoptosis and autophagy of ESCC cells, and inhibits subcutaneous tumor growth in nude mice. The mechanism might be related to the inhibition of PI3K/AKT/mTOR signaling pathway.
[Effects of knockdown ACC1 on glioma U251 cell migration and its mechanisms].
Zhang L, Qian H, Zhao BS … +2 more , Gao MQ, Liu YZ
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308429 · Publisher ↗
To investigate the effects of ACC1 knockdown on human glioma U251 cell migration and its molecular mechanisms. Human glioma U251 cell line was used. The experiment was carried out in three steps. Experiment 1: knockdown...
To investigate the effects of ACC1 knockdown on human glioma U251 cell migration and its molecular mechanisms. Human glioma U251 cell line was used. The experiment was carried out in three steps. Experiment 1: knockdown of ACC1 in U251 cells (shACC1) and its control (NC) U251 cells were established by transfection of shACC1 lentivirus and negative control virus. The cell migration was detected by Transwell migration assay and scratch test. Western blot (WB) was performed to detect the levels of ACC1, Vimentin, Fibronectin, N-cadherin, E-cadherin and Slug proteins. Experiment 2: RT-qPCR and WB were performed to verify the RNA-seq result, upregulation effect of ACC1 knockdown on PAI-1 in U251 cells. The cells then were treated with PAI-1 inhibitor PAI-039, and the cell migration was detected by Transwell migration assay and scratch assay. The protein levels of ACC1, PAI-1, Vimentin, Fibronectin, N-cadherin, E-cadherin and Slug were examined by WB. Experiment 3: the molecular mechanisms of knocking down ACC1 to increase PAI-1 were explored. The cells were treated with acetyltransferase inhibitor C646, and cell migration was examined by Transwell migration assay and scratch assay. WB was conducted to test the levels of ACC1, H3K9ac, PAI-1, Vimentin, Fibronectin, N-cadherin, E-cadherin and Slug proteins. Each experiment was repeated three times. Experiment 1: lentivirus transfection was performed on glioma U251 cells. Compared with NC group, the expression level of ACC1 in shACC1 group was decreased significantly, indicating that lentivirus transfection was successful (＜0.01), and the number of migrated cells in shACC1 group was increased significantly (＜0.01). Migration-related proteins Vimentin, Fibronectin, N-cadherin and Slug were up-regulated, while E-cadherin was down-regulated (＜0.01). Experiment 2: Compared with NC group, PAI-1 mRNA level in shACC1 group was up-regulated. Compared with control group, cell migration in shACC1+PAI-039 group was decreased (＜0.01), and migration-related proteins Vimentin, Fibronectin, N-cadherin, and Slug were up-regulated. E-cadherin expression was down-regulated (＜0.01). Experiment 3: Compared with NC group, the concentration of acetyl-coA and the expression level of H3K9ac in shACC1 group were increased significantly (＜0.01); After further treatment with histone acetyl transferase inhibitor C646, PAI-1 mRNA level was decreased, cell migration number and H3K9ac expression level were decreased in shACC1+C646 group compared with control group (＜0.01). Migration-related proteins Vimentin, Fibronectin, N-cadherin and Slug were up-regulated, while E-cadherin was down-regulated (＜0.01). Knockdown of ACC1 promotes the migration of human glioma U251 cells by increasing histone acetylation which elevates the level of PAI-1.
[Effects of fucoidan inducing impairment of human osteosarcoma cell 143B and its mechanism].
Wang QQ, Lin Q, Shan WY … +3 more , Zhang T, Li YR, Zhang Y
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2022 Nov · PMID 37308428 · Publisher ↗
To investigate the effects of fucoidan inducing impairment of human osteosarcoma cell 143B, as well its mechanisms. After 143B cells were treated with different concentrations of FUC (0, 0.5, 1, 10, 100, 400, 800 μg/ml)...
To investigate the effects of fucoidan inducing impairment of human osteosarcoma cell 143B, as well its mechanisms. After 143B cells were treated with different concentrations of FUC (0, 0.5, 1, 10, 100, 400, 800 μg/ml) for 48 h, the cell viability and dehydrogenase (LDH) level were detected by MTT assay and chemical colorimetry with six multiple wells for each concentration. Based on MTT results, we determined the value of IC was 244.5 μg/ml. The follow-up experiments were divided into control group (without FUC), FUC (10 μg/ml)-treated group, FUC (100 μg/ml)-treated group, FUC (400 μg/ml)-treated group and positive group (resveratrol, 40 μmol/L). There were four multiple wells for each concentration, and each experiment was repeated at least three times. Flow cytometry was performed to detect cell apoptosis and intracellular reactive oxygen species (ROS) level; acridine orange (AO) staining and lyso-tracker red staining were used to observe the autophagolysosome formation; chemical colorimetric analysis was performed to determine malondialdehyde (MDA) content and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px); Western blot was used to detect protein expressions of nuclear factor E2-associated factor 2 (Nrf2), heme oxygenase 1 (HO-1) and autophagy-associated proteins including microtubule-associated light chain protein 3 (LC-3), Atg7, Beclin-1 and p62. Compared with control group, the cell viability was decreased significantly in FUC (100～400 μg/ml)-treated groups (＜0.01); LDH levels in the supernatant (＜0.05 or ＜0.01), the percentage of cell apoptosis (＜0.01), intracellular ROS level and MDA content (＜0.01) were increased remarkably; protein expressions of Atg7 and Beclin-1 were upregulated (＜0.05 or ＜0.01); the conversion from LC-3I to LC-3II was significant (＜0.01) together with elevation of autophagolysosome formation (＜0.05 or ＜0.01); while the activities of SOD and GSH-Px and protein expressions of Nrf2, HO-1 and p62 were decreased remarkably (＜0.05 or ＜0.01). FUC (100～400 μg/ml) treatment induces oxidative damage and autophagic death in osteosarcoma 143B cells.
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