Más Lago P, Díaz J, Díaz Gonzalez M
… +6 more, Goyenechea Hernández A, Barrio Olivera J, Fonseca Quintana M, Morier Díaz L, Sarmiento Pérez L, Palomera Puente R
Rev Cubana Med Trop
· 2005 · PMID 17966580
Samples of feces and sera obtained from 3-year-old children were studied to increase the knowledge about the circulations of virus vaccines during the massive campaigns. The use of the oral polio vaccine with schemes of...Samples of feces and sera obtained from 3-year-old children were studied to increase the knowledge about the circulations of virus vaccines during the massive campaigns. The use of the oral polio vaccine with schemes of massive campaigns allows the circulation of the virus vaccine 2 months after their completion. The use of continual vaccination schemes makes possible the circulation of the virus vaccine for longer periods of time. Even in populations with a low immunity coverage, epidemic outbreaks of the vaccine-derived virus may appear. The total of poliovirus vaccine isolated in 2-year-old children (11 cases, 11.0 %) and the boosts of neutralizing antibodies (51 cases, 51.0 %), show a contradiction between the verification of the infections caused by isolations of the viruses and the results of boosts. The low percentage of isolations of virus vaccine and the highly significant percentages of of seroconversions or boosts to polio virus, allow to infer the occurrence of silent circulation. The silent circulation self limited to 2 months after concluding the campaign is due, among other causes, to the homologous or not induced response by the primary infection with the first dose of oral polio vaccine and by the secondary infections. The self limitation of the circulation of the polio viruses in massive campaigns constitutes an excellent prevention of the risks represented by the vaccine-derived viruses appearing in vaccinations with continual schemes.
Aguilar Barroso A, Amin Blanco N, Morier Díaz L
… +1 more, Pérez Hernández EM
Rev Cubana Med Trop
· 2005 · PMID 17966579
Viral infectivity of Hawaii, 3 Peri and Riberao Pretto dengue 1 strains was evaluated in Vero and HepG2 cell lines by indirect immunofluorescence techniques. Dengue virus cellular tropism in vitro is diverse. They may re...Viral infectivity of Hawaii, 3 Peri and Riberao Pretto dengue 1 strains was evaluated in Vero and HepG2 cell lines by indirect immunofluorescence techniques. Dengue virus cellular tropism in vitro is diverse. They may replicate themselves in a great variety of cellular cultures, whose sensibility to viral infection is variable. The greatest percentage of infected cells in the HepG2 cell line was obtained with the highest multiplicities of infection (0.04 for Hawaii and Riberao Pretto strains and 0.01 for 3 Perú). The highest percentage of infected HepG2 and Vero cells for the studied strains and the greatest titer in the viral overnadant was obtained on the 5th day. Vero cell line was more sensitive to viral infection, since for the same multiplicity values it was detected a higher number of fluorescent cells and a better viral titre in the line of this overnadant than in the HepG2. The best result was obtained with the Hawaii strain that allowed to confirm faster the infection of the studied cellular lines.
María García H, Año G, Cedré B
… +6 more, Valmaseda T, Maestre JL, Díaz M, Mirabal M, Talavera A, García L
Rev Cubana Med Trop
· 2005 · PMID 17966578
A methodology was developed for the selection of genetically modified strains of Vibrio cholerae 01 and 0139 aimed at obtaining oral attenuated candidate vaccines against cholera. The modified strains underwent microbiol...A methodology was developed for the selection of genetically modified strains of Vibrio cholerae 01 and 0139 aimed at obtaining oral attenuated candidate vaccines against cholera. The modified strains underwent microbiological characterization, bacterial susceptibility and different biological tests (mean lethal dose, colonizing capacity, adherence in mice, ligated intestine and intraduodenal inoculation in rabbits as virulence and potency tests. The strains 81, 638, 638T and 1333 were evaluated in clinical trials to determine their reactogenicity and immunogenicity. All the strains were sensitive to tetracycline and doxoclycine. They showed their attenuation and immunogenicity in animal models. The strains 638 and 1333 proved to be immunogenic and non reactogenic in volunteers.
Longa A, Vizcaya L, Nieves B
… +4 more, Bravo L, Morier L, Pérez-Schael I, Enrique Cabrera L
Rev Cubana Med Trop
· 2005 · PMID 17966577
The feces of 397 patients with acute diarrheal disease (ADD) and of other 121 patients without diarrea (control group) were studied in the state of Mérida, Venezuela, from June 1993 to December 1994. The genus Aeromonas...The feces of 397 patients with acute diarrheal disease (ADD) and of other 121 patients without diarrea (control group) were studied in the state of Mérida, Venezuela, from June 1993 to December 1994. The genus Aeromonas was identified in patients with ADD in 11.83% and in 5.78% of the patients from the control group. On studying the virulence factors described for Aeromonas (enterotoxin, cytotoxin, hemaglutinins, cellular hydrofibrosity, and hemolytic activity) in the isolated strains, it was detected that all presented at least one of the factors investigated associated with enteropathogenicity. Of the isolated species, Aeromonas caviae was the most frequently identified. All these results suggest that the Aeromonas species are potential enteric pathogens in this population.
Hernández MS, Ferrer JM, Oval CD
… +1 more, Sánchez JM
Rev Cubana Med Trop
· 2005 · PMID 17966493
A descriptive study was conducted to evaluate the outbreaks of the disease occurred in human beings and animals in the province of Ciego de Avila from 1980 to 2003. It was proved in this paper that the measures taken in...A descriptive study was conducted to evaluate the outbreaks of the disease occurred in human beings and animals in the province of Ciego de Avila from 1980 to 2003. It was proved in this paper that the measures taken in animal health and public health have managed to reduce the frequency of outbreaks.
26 patients with active posterior uveitis were studied by methodologies for the confirmation of leptospirosis. ELISA-IgG was positive in 10 patients and MAT in 21. Leptospiras were observed in blood and urine. The result...26 patients with active posterior uveitis were studied by methodologies for the confirmation of leptospirosis. ELISA-IgG was positive in 10 patients and MAT in 21. Leptospiras were observed in blood and urine. The results suggest that posterior uveitis may be caused by pathogenic leptospiras.
Velasco-Castrejón O, Rivas-Sánchez B, Espinoza J
… +2 more, Jiménez J, Ruiz E
Rev Cubana Med Trop
· 2005 · PMID 17966491
400 patients were studied by microscopic videorecording in dark field and MAT. The videorecording in blood was positive in 93 % and, in urine, in 95 %. Of the total of cases, 96 % presented antibody titers. The wide symp...400 patients were studied by microscopic videorecording in dark field and MAT. The videorecording in blood was positive in 93 % and, in urine, in 95 %. Of the total of cases, 96 % presented antibody titers. The wide symptomatic range gave origin to more than 50 different diagnoses in the studied patients, who were confirmed that they suffered from chronic leptospirosis by the diverse tests made.
A Leptospira strain was isolated from a hemoculture of a patient admitted with suspicion of leptospirosis that had been previously vaccinated. It corresponded to the Bataviae pathogenic complex. This result shows that th...A Leptospira strain was isolated from a hemoculture of a patient admitted with suspicion of leptospirosis that had been previously vaccinated. It corresponded to the Bataviae pathogenic complex. This result shows that there exists immunogenic specificity and that other serogroups should be incorporated to the current vaccine.
The operational conditions for the production of vaccine vax-SPIRAL were established at the scale of 100 L. It was concluded that it should be carried out with an air flow of 10 L/min (0.1 vvm) and without mechanical agi...The operational conditions for the production of vaccine vax-SPIRAL were established at the scale of 100 L. It was concluded that it should be carried out with an air flow of 10 L/min (0.1 vvm) and without mechanical agitation.
It was proposed to substitute the present technology of the lavage operation for a tangential microfiltration system at the lavage stages of the culture of Leptospira interrogans canicola canicola. It was proved that thi...It was proposed to substitute the present technology of the lavage operation for a tangential microfiltration system at the lavage stages of the culture of Leptospira interrogans canicola canicola. It was proved that this system may be applied, since no cellular affectation was observed and high pollutant elimination levels were obtained.
Núñez JF, Fajardo EM, Pérez E
… +3 more, Ontivero I, Silva D, Muñoz P
Rev Cubana Med Trop
· 2005 · PMID 17966487
Two different potency tests were evaluated, consisting in one-dilution used to determine 50% lethal dose (LD50) and four--dilution used to calculate 50% effective dose (ED50), respectively. Our experience has led to prop...Two different potency tests were evaluated, consisting in one-dilution used to determine 50% lethal dose (LD50) and four--dilution used to calculate 50% effective dose (ED50), respectively. Our experience has led to propose the four-dilution test for routine lot releasing.
The single-dose toxicological test for the leptospirosis vaccine vax-SPIRAL was made in rats. No clinical toxicity symptoms were found. The differences between the evaluated variables were not important from the biologic...The single-dose toxicological test for the leptospirosis vaccine vax-SPIRAL was made in rats. No clinical toxicity symptoms were found. The differences between the evaluated variables were not important from the biological point of view.
Two vacine candidate strains from Ballum serogroup were characterized and their immunogenicity and protective power were studied in hamster. The monovalent vaccine formulations obtained showed a high immunogenicity and c...Two vacine candidate strains from Ballum serogroup were characterized and their immunogenicity and protective power were studied in hamster. The monovalent vaccine formulations obtained showed a high immunogenicity and capacity of homologous protection and, in a lesser degree, of cross protection.
Chapola EG, dos Santos Md, Bessa TA
… +1 more, de Oliveira ML
Rev Cubana Med Trop
· 2005 · PMID 17966484
Sera from humans and dogs were tested by microscopic agglutination test. Icterohaemorrhagiae was the predominant serogroup in both groups, associated with the infestation of Rattus norvegicus.Sera from humans and dogs were tested by microscopic agglutination test. Icterohaemorrhagiae was the predominant serogroup in both groups, associated with the infestation of Rattus norvegicus.
It was studied the probable location of infection (PLI/area) and ambience of occurrence of human Leptospirosis cases, confirmed by laboratory report in Brazil from 2001 to 2003. The disease had more connection to the urb...It was studied the probable location of infection (PLI/area) and ambience of occurrence of human Leptospirosis cases, confirmed by laboratory report in Brazil from 2001 to 2003. The disease had more connection to the urban and suburban areas, suggesting that the occurrence of the disease is related to the life conditions and housing sanitation.
Serna JC, Ortiz JR, Servin AC
… +1 more, Espinoza AJ
Rev Cubana Med Trop
· 2005 · PMID 17966482
The prevalence of leptospirosis found in icteric patients negative to serological markers for hepatitis was described. 15.99 % of them were positive patients. It was observed a predominance of serovars canicola, pomona a...The prevalence of leptospirosis found in icteric patients negative to serological markers for hepatitis was described. 15.99 % of them were positive patients. It was observed a predominance of serovars canicola, pomona and icterohaemorrhagiae.
The effect of different alkalizing agents of urine on the isolation of leptospiras was studied. Better results were obtained on using the basic solution of NaOH 1N inoculated in urine one hour after being alkalized.The effect of different alkalizing agents of urine on the isolation of leptospiras was studied. Better results were obtained on using the basic solution of NaOH 1N inoculated in urine one hour after being alkalized.
Rivera YF, Navarro YP, Mazorra YA
… +3 more, Rodríguez AG, Padrón Mde L, Valdés Y
Rev Cubana Med Trop
· 2005 · PMID 17966480
Two detergents (triton X-100 and sodium desoxycholate) were evaluated as solubilizing agents of the components of the outer membrane of Leptospira Pomona serovar mozdok. It was obtained a greater yielding of proteins on...Two detergents (triton X-100 and sodium desoxycholate) were evaluated as solubilizing agents of the components of the outer membrane of Leptospira Pomona serovar mozdok. It was obtained a greater yielding of proteins on applying the treatment with triton X-100. The variants formulated starting from the extracts obtained showed a high immunogenicity and capacity of homologous protection against the challenge in hamster.
Vitale M, Vitale F, Reale S
… +3 more, Vesco G, Currò V, Caracappa S
Rev Cubana Med Trop
· 2005 · PMID 17966479
It was carried out a comparison on two reference Leptospira strains DNA between different extraction methods and two polymerase chain reaction protocol. The DNA was quantified and serial dilutions were tested by polymera...It was carried out a comparison on two reference Leptospira strains DNA between different extraction methods and two polymerase chain reaction protocol. The DNA was quantified and serial dilutions were tested by polymerase chain reaction. The results showed difference in terms of recovery and sensitivity between this methods.
Theodoridis D, Böhmer J, Homuth M
… +1 more, Strutzberg-Minder K
Rev Cubana Med Trop
· 2005 · PMID 17966478
A multi-serovar ELISA based on the outer membrane Lipoprotein L41 (LipL41) of pathogenic Leptospira was developed to increase sensitivity by using a single test antigen. A sensitivity of 99 % and a specifity of 92 % coul...A multi-serovar ELISA based on the outer membrane Lipoprotein L41 (LipL41) of pathogenic Leptospira was developed to increase sensitivity by using a single test antigen. A sensitivity of 99 % and a specifity of 92 % could be achieved. The established diagnostic polymerase chain reaction is also able to detect fast and reliably pathogenic Leptospira in different clinical samples.