Essid A, Elbini I, Ksiksi R
… +7 more, Harrab N, Moslah W, Jendoubi I, Doghri R, Zid MF, Luis J, Srairi-Abid N
Bioinorg Chem Appl
· 2025 · PMID 39834888
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The efficacy of available treatments for melanoma is limited by side effects and the rapidly emerging resistance to treatment. In this context, the decavanadate compounds represent promising tools to design efficient the...The efficacy of available treatments for melanoma is limited by side effects and the rapidly emerging resistance to treatment. In this context, the decavanadate compounds represent promising tools to design efficient therapeutic agents. In our study, we synthesized a dimagnesium disodium decavanadate icosahydrate compound (MgNaVO·20HO) and investigated its structure stability as well as its antimelanoma effects. Results showed that the MgNaVO·20HO compound is structured in a monoclinic system with the space group C2/c, stabilized by oxygen vertices, hydrogen bonds, and van der Waals interactions. Interestingly, we found that this newly synthesized compound reduced the viability of human (IGR39, IGR37, and SKMEL28) and murine (B16-F10) melanoma cells in a dose-dependent manner. The IC values ranged from 7.3 to 18 μM after 24 h and decreased to 1.4 μM after 72 h of treatment. Notably, this effect was more important than that of cisplatin (IC = 3 μM after 72 h of treatment), a chemotherapeutic agent, commonly used in the treatment of malignant melanoma. Furthermore, the cytotoxicity of the decavanadate compound was relatively weak on normal human keratinocytes (HaCaT), with a light effect (IC >> 70 μM) observed after 24 h of treatment. Thus, the MgNaVO·20HO compound displayed an advantage over cisplatin, which was reported to be much more aggressive to the keratinocyte cell line (IC = 23.9 μM). Moreover, it inhibited dose-dependently the adhesion of IGR39 cells to collagen (IC = 2.67 μM) and fibronectin, as well as their migration with an IC value of 2.23 μM. More interestingly, its in vivo administration to B16-F10 allografted mice, at the nontoxic dose of 50 μg (2.5 mg/kg), prevented and suppressed by 70% the tumor growth, compared to the nontreated mice. Moreover, this compound has also allowed a recovery against inflammation induced in mice by a mixture of DMBA and croton oil. Thus, all our results showed the potential of the MgNaVO·20HO compound to prevent and efficiently treat the growth and metastasis of melanoma.
Al Musayeib NM, Amina M, Maqsood F
… +2 more, Bokhary KA, Alrashidi NS
Bioinorg Chem Appl
· 2024 · PMID 38962162
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BACKGROUND: Magnesium oxide nanoparticles (MgONPs) have been fabricated by several approaches, including green chemistry approach due to diverse application and versatile features. OBJECTIVES: The current study aimed to...BACKGROUND: Magnesium oxide nanoparticles (MgONPs) have been fabricated by several approaches, including green chemistry approach due to diverse application and versatile features. OBJECTIVES: The current study aimed to prepare a convenient, biocompatible, and economically viable MgONPs using waste citron peel extract (CP-MgONPs) to evaluate their biological applications. METHODS: The CP-MgONPs were synthesized by a sustainable approach from extract of waste citron peel both as capping and reducing agents without use of any hazardous material. The physicochemical features of formed CP-MgONPs were determined by sophisticated analytical and microscopic techniques. The biogenic CP-MgONPs were examined for their antibacterial, anticarcinogenic, and photocatalytic attributes. RESULTS: A prominent absorption peak in the UV-Vis spectra at 284 nm was the distinguishing characteristic of the CP-MgONPs. The scanning electron microscopy (SEM) reveals polyhedral morphology of nanoparticles with slight agglomeration of CP-MgONPs. The CP-MgONPs exerted excellent antibacterial potencies against six bacterial strains. The CP-MgONPs displayed significant susceptibility towards (20.72 ± 0.33 mm) and (19.52 ± 0.05 mm) with the highest inhibition zones. The anticancer effect of CP-MgONPs was evaluated against HepG2 (IC : 15.3 g·mL) cancer cells and exhibited potential anticancer activity. A prompt inversion of cellular injury manifested as impairment of the integrity of the cell membrane, apoptosis, and oxidative stress was observed in treated cells with CP-MgONPs. The biosynthesized CP-MgONPs also conducted successful photocatalytic potential as much as MgO powder under the UV-light using acid orange 8 (AO-8) dye. The degradation performance of CP-MgONPs showed over 94% photocatalytic degradation efficiency of acid orange 8 (AO-8) dyes within a short time. CONCLUSIONS: Outcomes of this research signify that biogenic CP-MgONPs may be advantageous at low concentrations, with positive environmental impacts.
Bioinorg Chem Appl
· 2024 · PMID 38840845
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X-ray crystallography, spectroscopy, computational methods, molecular docking studies, and DNA-binding studies have been useful in the investigations of intermolecular and intramolecular interactions of osmium-cymene ox...X-ray crystallography, spectroscopy, computational methods, molecular docking studies, and DNA-binding studies have been useful in the investigations of intermolecular and intramolecular interactions of osmium-cymene oxalato complexes with aryl phosphine and aryl phosphonium groups in both primary and secondary coordination spheres, respectively. Molecular structures of the novel complexes PPh[Os(--cymene)Br(--CO)] () and [Os(--cymene) (--CO)PPh] () were resolved by single-crystal X-ray diffraction (XRD). Primary and secondary coordination sphere contacts were investigated using Hirshfeld surface analysis which was supported by molecular docking (MD) studies. The MD data obtained predicted significant differences in binding energy across three receptors for the two osmium complexes. An DNA-binding study was accomplished using UV-Vis spectroscopy which showed that both and bond with DNA through an intercalation approach. The optimized molecular geometry, frontier molecular orbital (E and E) energies, global electrophilicity index (), chemical hardness (), chemical potential (), and the energy band gap (E-E) were calculated utilizing density functional theory (DFT) methods. Computed structural parameters (bond lengths and angles) support the experimental single-crystal XRD data.
Reddy SM, Karmankar SB, Alzahrani HA
… +5 more, Hadap A, Iqbal A, Alenazy R, Salem-Bekhit MM, Jain B
Bioinorg Chem Appl
· 2023 · PMID 38623482
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Zinc molybdate nanoparticles with molybdate are synthesized through green method with different salt precursors using leaf extract. Those nanoparticles had structural, vibrational, and morphological properties, which we...Zinc molybdate nanoparticles with molybdate are synthesized through green method with different salt precursors using leaf extract. Those nanoparticles had structural, vibrational, and morphological properties, which were determined by X-ray diffraction (XRD). The crystalline size of synthesized zinc molybdate was 24.9 nm. Fourier transform infrared (FTIR) spectroscopy and field emission scanning electron microscopy (FE-SEM) clearly showed the attachment of molybdate with ZnO. The synthesized nanomaterial was also characterized through UV-visible spectroscopy which had 4.40 eV band gap energy. Those nanoparticles were also characterized via thermogravimetric analysis (TGA-DTA) and photoluminance spectroscopy (PL). ZnMoO had photocatalytic property via methylene blue dye. After 190 minutes, the dye changed to colourless from blue colour. The degradation efficiency was around 92.8%. It also showed their antibacterial effect via and bacterial strains. In the presence of light and air, nanoparticles of ZnMoO inhibit the growth of cells of and bacterial strains because of ROS (reactive oxygen species) generation. Because of the formation of singlet oxygen (O), hydrogen oxide radical (-OH), and hydrogen peroxide (HO), ZnMoO showed photodegradation reaction against aq. solution of methylene blue dye at 6 pH with constant time interval. With time, the activity of ZnMoO also decreased because of the generation of a layer of hydrogen oxide (-OH) on nanomaterial surface, which could be washed with ethanol and distilled water. After drying, the catalytic Zinc molybdate nanoparticles could be reused again in the next catalytic reaction.
Blázquez-Tapias B, Halder S, Mendiola MA
… +5 more, Roy N, Sahu N, Sinha C, Jana K, López-Torres E
Bioinorg Chem Appl
· 2024 · PMID 38601021
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Nowadays, the search for new chemotherapeutic agents with low toxicity and high selectivity is a major concern. In this paper, we report the synthesis and characterization of a hybrid thiosemicarbazone/hydrazone ligand i...Nowadays, the search for new chemotherapeutic agents with low toxicity and high selectivity is a major concern. In this paper, we report the synthesis and characterization of a hybrid thiosemicarbazone/hydrazone ligand in its neutral form (LH) and as the chloride salt ([LH]Cl)-, three diorganotin (IV) complexes, and one complex with Sn (IV). The compounds have been fully characterized by IR, mass spectra, H, C, and Sn NMR, Sn CP/MAS NMR, and by single crystal X-ray diffraction. The organotin compounds have the empirical formula [SnRL] ( = Me, Bu, and Ph), but in the solid state, they are polymeric species with seven coordination number due to weak coordination of the pyridine nitrogen, whereas in solution, the polymeric structure is lost to afford hexacoordinate monomeric species. Reaction with SnI yields complex [Sn (L)]·EtOH, with the metal in a distorted dodecahedral arrangement. We have evaluated the antiproliferative activity of the two forms of the ligands and the four coordination compounds against MDA-MB-231, HeLa, PC3, and HepG2 cancer cell lines, and WI-38 normal cell line, and all the compounds present higher activity than cisplatin, used as the standard control. To investigate the mode of action, we have selected the most active complex, containing phenyl substituents, and used the triple negative breast cancer cell line MDA-MB-231. The results show that the complex induces apoptotic cell death promoted by generation of reactive oxygen species and by disruption of mitochondrial membrane potential.
Bioinorg Chem Appl
· 2024 · PMID 38380152
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Carrier system therapies based on combining cancer drugs with nanoparticles have been reported to control tumor growth and significantly reduce the side effects of cancer drugs. We thought that paclitaxel-loaded silver n...Carrier system therapies based on combining cancer drugs with nanoparticles have been reported to control tumor growth and significantly reduce the side effects of cancer drugs. We thought that paclitaxel-loaded silver nanoparticles (AgNPs-PTX) were the right carrier to target cancer cells. We also carried out antimicrobial activity experiments as systems formed with nanoparticles have been shown to have antimicrobial activity. In our study, we used easy-to-synthesize and low-cost silver nanoparticles (AgNPs) with biocatalytic and photocatalytic advantages as drug carriers. We investigated the antiproliferative activities of silver nanoparticles synthesized by adding paclitaxel on MCF-7 (breast adenocarcinoma cell line), A549 (lung carcinoma cell line), C6 (brain glioma cell line) cells, and healthy WI-38 (fibroblast normal cell line) cell lines and their antimicrobial activities on 10 different microorganisms. The synthesized AgNPs and AgNPs-PTX were characterized by dynamic light scattering (DLS), scanning transmission electron microscopy, UV-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray spectroscopy. The nanoparticles were spherical in shape, with AgNPs ranging in size from 2.32 to 5.6 nm and AgNPs-PTXs from 24.36 to 58.77 nm. AgNPs demonstrated well stability of -47.3 mV, and AgNPs-PTX showed good stability of -25.4 mV. The antiproliferative effects of the synthesized nanoparticles were determined by XTT (tetrazolium dye; 2,3-bis-(2-methoxy-4-nitro-5-sulfenyl)-(2H)-tetrazolium-5-carboxanilide), and the proapoptotic effects were determined by annexin V/propidium iodide (PI) staining. The effect of AgNPs-PTX was more effective, and anticancer activity was higher than PTX in all cell lines. When selectivity indices were calculated, AgNPs-PTX was more selective in the A549 cell line (SI value 6.53 g/mL). AgNPs-PTX was determined to increase apoptosis cells by inducing DNA fragmentation. To determine the antimicrobial activity, the MIC (minimum inhibitory concentration) test was performed using 8 different bacteria and 2 different fungi. Seven of the 10 microorganisms tested exhibited high antimicrobial activity according to the MIC ≤100 g/mL standard, reaching MIC values below 100 g/mL and 100 g/mL for both AgNPs and AgNPs-PTX compared to reference sources. Compared to standard antibiotics, AgNPs-PTX was highly effective against 4 microorganisms.
Li J, Wang Y, Tao J
… +6 more, Su X, Zhu F, Lu W, Han X, Dang M, Weng L
Bioinorg Chem Appl
· 2024 · PMID 38333411
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Photodynamic therapy (PDT) has received increasing attention for tumor therapy due to its minimal invasiveness and spatiotemporal selectivity. However, the poor targeting of photosensitizer and hypoxia of the tumor micro...Photodynamic therapy (PDT) has received increasing attention for tumor therapy due to its minimal invasiveness and spatiotemporal selectivity. However, the poor targeting of photosensitizer and hypoxia of the tumor microenvironment limit the PDT efficacy. Herein, eccentric hollow mesoporous organic silica nanoparticles (EHMONs) are prepared by anisotropic encapsulation and hydrothermal etching for constructing PDT nanoplatforms with targeting and hypoxia-alleviating properties. The prepared EHMONs possess a unique eccentric hollow structure, a uniform size (300 nm), a large cavity, and ordered mesoporous channels (2.3 nm). The EHMONs are modified with the mitochondria-targeting molecule triphenylphosphine (CTPP) and photosensitizers chlorin e6 (Ce6). Oxygen-carrying compound perfluorocarbons (PFCs) are further loaded in the internal cavity of EHMONs. Hemolytic assays and toxicity experiments show that the EHMONs-Ce6-CTPP possesses very good biocompatibility and can target mitochondria of triple-negative breast cancer, thus increasing the accumulation of photosensitizers Ce6 at mitochondria after entering cancer cells. The EHMONs-Ce6-CTPP@PFCs with oxygen-carrying ability can alleviate hypoxia after entering in the cancer cell. Phantom and cellular experiments show that the EHMONs-Ce6-CTPP@PFCs produce more singlet oxygen reactive oxygen species (ROSs). Thus, in vitro and in vivo experiments demonstrated that the EHMONs-Ce6-CTPP@PFCs showed excellent treatment effects for triple-negative breast cancer. This research provides a new method for a targeting and oxygen-carrying nanoplatform for enhancing PDF effectiveness.