Cellular And Molecular Biology (Noisy-le-Grand, France)[JOURNAL]
Sun
200 papers
Therapeutic potential of kodo millet in rheumatoid arthritis through immunomodulation and antioxidant enhancement.
Abhirami Sunitha, Amrutha Dileep Kumar Sreeja Kumari, Sebastian M … +4 more , Haritha Rajan, Maya Gopinathan Pillai, Salu Valsala Sasi Kumar, Antony Helen
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236301 · Publisher ↗
Rheumatoid arthritis (RA) is a chronic autoimmune disease, and conventional medications used to treat RA have anti-inflammatory activity as one of their primary modes of action. Since medications have unfavorable side e...
Rheumatoid arthritis (RA) is a chronic autoimmune disease, and conventional medications used to treat RA have anti-inflammatory activity as one of their primary modes of action. Since medications have unfavorable side effects, dietary therapy for arthritis is presently getting lots of attention. Kodo millet (Paspalum scorbiculatum) is a highly nutritious grain belonging to the Poaceae family, rich in fiber, nutrients and antioxidant content. They are the least exploited cereals and have pharmacological benefits in various conditions. The present study reveals the potential effects of kodo millet on immune regulation and antioxidant defense in collagen-induced arthritic (CIA) rats. Female Wistar rats were divided into three groups. Group I- Normal, Group II -CIA, Group III- CIA+ 10% cooked kodo millet (KM). After 60 days of experimental period and millet supplementation, organ indices, B-cell, Th2, Th17 immune responses, RANKL/NFATc pathways, cytokines and level of antioxidants were analyzed. Our findings reveal that 10% cooked kodo millet enhances B cell functionality, Th2-Th17 immunocyte balance. It attenuates inflammation by modulating RANKL/NFATc pathway, resulting in decreased proinflammatory mediators, increased levels of anti-inflammatory cytokines and improved tissue integrity. Millet inhibited ROS production and boosted antioxidant status via increasing SOD and GSH and decreasing activity of MPO, MDA and NO. The results highlight the potential of kodo millet as a functional food to manage the progression of rheumatoid arthritis.
Molecular identification of yeasts involved in the alcoholic fermentation of Tchoukoutou and Atan in Benin using sequencing.
Nanoukon CNM, Tchekessi C, Badoussi M … +8 more , Accrombessi B, Ohin B, N'tcha C, Havivi A, Déguénon L, Loumèdjinon P, Baba-Moussa F, Djèdatin LG
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236300 · Publisher ↗
In Benin, the consumption of local beverages is increasing, particularly "Tchoukoutou" and "Atan". However, they face numerous challenges that hinder their recognition as fermented drinks with potential medical and cultu...
In Benin, the consumption of local beverages is increasing, particularly "Tchoukoutou" and "Atan". However, they face numerous challenges that hinder their recognition as fermented drinks with potential medical and cultural benefits. This study aims to determine the biodiversity of yeasts responsible for the fermentation of "Tchoukoutou" and "Atan" produced in Benin to promote their production and commercialization. To achieve this, beverage samples were randomly collected from six (06) municipalities in Benin and subjected to physicochemical and microbiological analysis. Yeast identification was performed through sequencing of the D1/D2 region of the 26S ribosomal RNA (26S rRNA) gene. Physicochemical analysis revealed that the pH values ranged between 3.65 and 3.92 for "Tchoukoutou" and between 3.71 and 4.04 for "Atan." Regarding sugar content (Brix degree), "Tchoukoutou" values ranged from 1 to 12° Brix, while those of "Atan" ranged from 1° to 14° Brix. Microbiological analysis showed that the yeast count in "Tchoukoutou" and "Atan" was 1.57 × 10³ and 5.29 × 10³ CFU/mL, respectively. A total of five (05) different yeast species were identified in "Tchoukoutou" and six (06) in "Atan". Schizosaccharomyces pombe (44.44%) was the predominant yeast species in the fermentation of "Tchoukoutou", followed by Saccharomyces cerevisiae (22.22%). In contrast, for "Atan", Saccharomyces cerevisiae (53.33%) was the most dominant species, followed by Saccharomyces boulardii (13.33%), and Candida parapsilosis (13.33%). Other yeast species present in lower percentages included Candida parapsilosis (11.11%), Pichia sp. Feni (11.11%) and Pichia manshurica (11.11%) in "Tchoukoutou" and Pichia kudriavzevii (6.66%), Pichia ethanolica (6.66%) and Schizosaccharomyces osmophilus (6.66%) in "Atan". These results indicate that Beninese "Tchoukoutou" and "Atan" fermentation is primarily driven by Schizosaccharomyces pombe, a yeast not commonly found in traditional Benin beverages, and Saccharomyces cerevisiae, respectively. This information is valuable for developing genetic engineering strategies to control their alcoholic fermentation, improve packaging, and extend shelf life.
Clinical significance of B7-H4 expression in ovarian cancer: a meta-analysis of proportions and time-to-event survival outcomes.
Varikasuvu SR, Sharma S, Banerjee P … +15 more , Kumar S, Varshney S, Gupta P, Mudgal SK, Lisa M, Sinha RK, Kumar N, Nishi, Lakra PS, Singh SK, Bohra HR, Vaithy A, Barla NPA, Sinha A, The Smart Centre-Brics Initiative For Capacity Building
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236299 · Publisher ↗
B7-H4 is an immune-regulatory molecule increasingly recognized for its role in tumor progression and immune evasion in epithelial ovarian cancer. To clarify its clinical relevance, we conducted a systematic review and me...
B7-H4 is an immune-regulatory molecule increasingly recognized for its role in tumor progression and immune evasion in epithelial ovarian cancer. To clarify its clinical relevance, we conducted a systematic review and meta-analysis evaluating the prevalence of B7-H4 expression and its association with survival outcomes. Nineteen eligible studies were included, of which sixteen provided data on expression proportions and eight reported progression-free or overall survival outcomes. The pooled prevalence of high or positive B7-H4 expression was 73%, though with considerable inter-study variability. High B7-H4 expression was associated with a significantly increased risk of disease progression (pooled unadjusted hazard ratio: 1.43), while its relationship with overall survival remained inconclusive due to limited data. Despite methodological differences among studies, the findings suggest B7-H4 is overexpressed and potentially prognostic in ovarian cancer. Additional studies are required to validate its clinical utility in patient risk assessment and as a therapeutic target.
Isolation and characterization of native strains from lithium-containing mining tailings.
Rentería-Ortiz KS, Medrano-Roldán H, Martínez-Rodríguez GA … +4 more , Vázquez-Ortega PG, Rojas-Contreras JA, Reyes-Jáquez D, Zazueta-Alvarez DE
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236298 · Publisher ↗
Lithium-rich mining tailings create extreme and poorly explored environments that can serve as reservoirs for microorganisms with unique biotechnological potential. In this study, native microorganisms were isolated and...
Lithium-rich mining tailings create extreme and poorly explored environments that can serve as reservoirs for microorganisms with unique biotechnological potential. In this study, native microorganisms were isolated and molecularly characterized from the tailings of the Nochebuena mine, Zacatecas, Mexico. Six pure cultures were obtained using serial dilution and cross-streaking methods. Lithium tolerance was assessed through minimum inhibitory concentration (MIC) assays, revealing resistance levels between 5,000 and 20,000 ppm LiCl. Molecular characterization involved DNA extraction, amplification, purification, and restriction digestion with the HhaI enzyme, followed by sequencing. The isolates were identified as Stutzerimonas chloritidismutans, Microbacterium paraoxydans, Stenotrophomonas maltophilia, Bacillus cereus, and Bacillus ludwigii. Phylogenetic analysis was performed to resolve their evolutionary relationships. These findings highlight the untapped microbial diversity in lithium-rich mining residues and its promise for innovative applications in biohydrometallurgy.
Evaluation of heat shock protein 70, AMH, and key hormonal markers in cellular mechanisms of polycystic ovary syndrome.
Khaleel AS, Al-Fatlawi ACY, Al-Safi WGA
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236297 · Publisher ↗
Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases characterized by hyperandrogenemia and anovulation. The present study aimed to estimate heat shock protein 70 (HSP-70) in PCOS patients. This...
Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases characterized by hyperandrogenemia and anovulation. The present study aimed to estimate heat shock protein 70 (HSP-70) in PCOS patients. This case-control study involved 90 females aged 15 to 45 years, divided into two groups: 45 controls and 45 PCOS patients. Levels of HSP-70, anti-Müllerian hormone (AMH), luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin, and testosterone were measured using enzyme-linked immunosorbent assay (ELISA). The study revealed a significant increase (p ≤ 0.01) in HSP-70 and AMH levels in PCOS patients compared to controls, with mean ± SD values of 16.49 ± 2.79 and 2386.14 ± 530.09, respectively. Hormonal parameters such as LH, testosterone, and prolactin were elevated, whereas FSH was decreased in PCOS patients. The findings suggest that elevated HSP-70 plays a key role in the pathogenesis of PCOS.
Genetic diversity and phylogenetic structure of Marawh and Bidah pomegranate landraces from Al-Baha, Saudi Arabia, using ITS DNA barcoding.
Albogami A
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236296 · Publisher ↗
Pomegranate (Punica granatum L.) plays a vital cultural and economic role in the Al-Baha region of Saudi Arabia. Despite its significance, limited molecular data exist on the genetic structure of local landraces, particu...
Pomegranate (Punica granatum L.) plays a vital cultural and economic role in the Al-Baha region of Saudi Arabia. Despite its significance, limited molecular data exist on the genetic structure of local landraces, particularly the distinct red and green fruit colour variants of the Marawh and Bidah cultivars. This study investigates the genetic diversity and phylogenetic relationships among these landraces using the nuclear internal transcribed spacer (ITS) DNA region. Maximum likelihood phylogenetic tree construction and network analysis (SplitsTree) were employed. Results reveal that red- and green-fruited landraces cluster into distinct clades, with red variants exhibiting reticulate patterns suggestive of introgression or incomplete lineage sorting. Genetic distance analysis confirmed a high similarity (~99.15%) between the green variants, despite their placement in separate clades. The findings provide crucial insights into the evolutionary history, cultivar authentication, and conservation strategies for pomegranate germplasm in Al-Baha. Future directions include genome-wide SNP analyses and expanded sampling to refine our understanding of these valuable genetic resources.
The role of tRNA-derived fragments in prostate cancer: a review.
Babaei S, Khorrami M, Eskandari A … +7 more , Ramazani M, Saffar Moghadam AA, Rezaei-Taviran M, Soleimani S, Ghadyani F, Sepehr Kahrizi, Movafagh A
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236295 · Publisher ↗
Prostate Cancer (PCa) is a leading malignancy in men in developed countries. The lack of reliable prognostic markers in PCa hinders effective treatment, leading to potential patient misclassification and overtreatment wi...
Prostate Cancer (PCa) is a leading malignancy in men in developed countries. The lack of reliable prognostic markers in PCa hinders effective treatment, leading to potential patient misclassification and overtreatment with associated side effects. Recent advances in high-throughput sequencing have enabled the identification of tRNA-derived fragments (tRFs), small non-coding RNAs derived from tRNA cleavage. tRFs regulate crucial cellular processes like viability, differentiation, and homeostasis, implicating them in disease development, particularly cancer. Their potential as biomarkers for early diagnosis and prognosis, as well as targets for precision therapies, is increasingly recognized. This review focuses on the key biological functions of tRFs, including RNA silencing, translation regulation, and epigenetic regulation. It summarizes recent findings on tRFs in PCa, exploring their potential as clinical biomarkers and therapeutic targets.
Association of cannabinoid gene polymorphism with neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1) in type 2 diabetes mellitus with chronic kidney disease.
Mohammed MZ, Nasir HM, Al-Shakir NMM
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236294 · Publisher ↗
Diabetic kidney disease (DKD) is a common microvascular complication of type 2 diabetes mellitus (T2DM) and a leading cause of end-stage renal disease (ESRD). Genetic factors, including polymorphisms in the cannabinoid r...
Diabetic kidney disease (DKD) is a common microvascular complication of type 2 diabetes mellitus (T2DM) and a leading cause of end-stage renal disease (ESRD). Genetic factors, including polymorphisms in the cannabinoid receptor 1 (CNR1) gene, may influence the risk and progression of chronic kidney disease (CKD) in diabetic patients. This study aimed to investigate the association of CNR1 gene polymorphisms, specifically single-nucleotide polymorphisms (SNPs) rs1049353 and rs1776966256, with serum levels of kidney injury biomarkers neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) in Iraqi patients with T2DM, with and without CKD. A total of 120 subjects were enrolled and divided into three groups: 40 T2DM patients with CKD, 40 T2DM patients without CKD, and 40 healthy controls. Genotyping was performed using conventional polymerase chain reaction followed by Sanger sequencing. Serum NGAL and KIM-1 levels were measured by ELISA. Multiple novel CNR1 gene variants were detected and submitted to the NCBI database. The heterozygous GA genotype of rs1049353 was more prevalent in the CKD group compared to others, although not statistically significant. The rs773947953 (G>A) variant showed significant association with CKD, where the A allele appeared protective. Significant correlations were also observed between NGAL, KIM-1 levels, and specific SNP genotypes, including rs773947953 and new variations at positions 4217 (G>A) and 4224 (rs2481890897). These findings suggest that CNR1 gene polymorphisms influence susceptibility to diabetic kidney injury and are associated with elevated tubular injury markers. Identification of these genetic variations may help in early prediction and personalized management of DKD.
Impact of regional and general aerobic exercise on molecular regulators of lipolysis and adipose tissue composition in obese women.
Zourmand G, Shaabani Ezdini E, Taheri M … +1 more , Irandoust K
Cell Mol Biol (Noisy-le-grand) · 2025 Nov · PMID 41236293 · Publisher ↗
This randomized controlled trial compared the effects of regional, general, and combined aerobic training on plasma lipolytic markers and subcutaneous fat reduction in obese women with abdominal or gluteofemoral fat accu...
This randomized controlled trial compared the effects of regional, general, and combined aerobic training on plasma lipolytic markers and subcutaneous fat reduction in obese women with abdominal or gluteofemoral fat accumulation. Sixty women (age 25-50 years; BMI ≥30 kg/m²) were assigned to general aerobic training (GATG; treadmill/cycling), regional aerobic training (RTG; targeted abdominal or lower-body rhythmic exercises), or combined training (CTG; 15 min general + 15 min regional), performed three times per week for 12 weeks at 55-75% heart rate reserve. Dietary intake was monitored to ensure no major changes. Anthropometric indices (weight, BMI, waist-to-hip ratio [WHR]) and plasma free fatty acids (FFAs), glycerol, and insulin were measured pre- and post-intervention. All groups demonstrated significant reductions in weight, BMI, WHR, and biochemical markers (p<0.05). The CTG achieved the greatest improvements: weight -6.38%, BMI -6.30%, WHR -13.13%, accompanied by the largest declines in FFAs, glycerol, and insulin. GATG showed superior systemic fat loss compared to RTG, but RTG still produced notable WHR and insulin reductions, suggesting a complementary role for localized exercise. The superior outcomes in CTG may reflect synergistic effects of global cardiovascular activation and targeted regional muscle engagement, potentially enhancing both systemic lipolysis and local adipose tissue responsiveness. Biochemical improvements, particularly reductions in fasting FFAs and glycerol, indicate enhanced adipocyte insulin sensitivity and lipid utilization. The pronounced insulin decline in CTG is clinically relevant for metabolic risk reduction. While "spot reduction" remains controversial, these findings support integrating regional movements within broader aerobic programs to address resistant fat depots. This study suggests that combining general and regional aerobic training offers a practical, scalable approach for improving metabolic health and body composition in obese women. Future research should employ imaging-based fat quantification, hormonal profiling, and long-term follow-up to clarify underlying mechanisms and optimize depot-specific exercise prescriptions.
TNF-α 308 (rs1800629) and INF-γ +874 polymorphisms in dengue progression: genotype-specific trends amidst allelic non-association in West Africa.
Tassembedo F, Tapsoba ASA, Bello SOT … +10 more , Kouta OF, Savadogo M, Ouedraogo RA, Ilboudo DP, Traore L, Yougbare F, Nagalo BM, Djigma FW, Diagbouga PS, Simpore J
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054377 · Publisher ↗
Dengue, transmitted by Aedes mosquitoes, represents a significant global health challenge due to its complex host-pathogen interactions and varying disease severity. Genetic factors are known to influence the clinical ou...
Dengue, transmitted by Aedes mosquitoes, represents a significant global health challenge due to its complex host-pathogen interactions and varying disease severity. Genetic factors are known to influence the clinical outcome of dengue infections. This study aimed to investigate the potential role of TNF-α gene polymorphism 308 (rs1800629) and INF-γ +874 (rs62559044) in the progression of dengue virus infection. Conducted in the Central region of Burkina Faso, this study included 246 participants, comprising 117 controls and 129 dengue-positive patients. Genotyping of the TNF-α 308 (rs1800629) and INF-γ +874 A/T (rs62559044) polymorphisms was performed using restriction fragment length polymorphism (RFLP) and Amplification Refractory Mutation System PCR (ARMS-PCR) techniques, respectively. Our analysis revealed no significant correlation between lymphocyte count and dengue severity (P = 0.95). Although we did not find an association between the alleles of the SNPs TNF-α 308 (rs1800629) and INF-γ +874 (rs6255904) studied with either DF or severe DS, we cannot conclude the same for their respective genotypes Thus, the AA and GG genotypes of TNF-α are associated with the contraction of DF and DS, respectively; the former is even associated with the progression of DF to the severe form of the disease. For INF-γ AA genotypes are more associated with progression to severe dengue and the AT heterozygote could be associated with a possibility of preventing progression to DS forms. . The A allele frequencies was higher frequency in DF than in DS pour TNF-α 308 , but this difference lacked statistical significance (P > 0.005). With INF-γ tTT genotype was more prevalent in DS, whereas the AT genotype frequencies differed between DF (23.96%) and DS (19.35%). Our results reveal through the allelic levels of TNF-α 308 and INF-γ +874; that the latter would not play a significant role in the progression of dengue virus infection to severe forms. However, previous studies through a clear mechanism show a strong association between the concentrations of these cytokines and the pathogenesis of dengue. This underlines the need for further investigations to elucidate the genetic determinants of the severity of dengue. In particular, a proteomic study coupled with sequencing on a representative population of the West African region would be a great asset in understanding the involvement of the genes of these cytokines in the pathogenesis of dengue.
LncRNA MEG3 overexpression modulates proliferation without inducing apoptosis in rat cardiomyoblast h9c2 cells: a transcriptomic approach.
Xing Z, Abudureyimu S, Abulaiti P … +4 more , Wang Y, Li H, Lyu M, Gao Y
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054376 · Publisher ↗
Long non-coding RNAs (lncRNAs) have been implicated in various biological processes including cell proliferation and apoptosis. However, the role of lncRNA-MEG3 in rat cardiomyoblast H9C2 cells remains unclear. In this s...
Long non-coding RNAs (lncRNAs) have been implicated in various biological processes including cell proliferation and apoptosis. However, the role of lncRNA-MEG3 in rat cardiomyoblast H9C2 cells remains unclear. In this study, H9C2 cells were genetically modified to overexpress lncRNA MEG3. The proliferation of these cells was evaluated using the Cell Counting Kit-8 (CCK-8) assay and direct imaging techniques. Apoptosis was assessed through flow cytometry, employing Annexin V and propidium iodide (PI) staining. Quantitative PCR was utilized to confirm the overexpression of lncRNA MEG3. Further, differential expression and alternative splicing analyses were conducted using comprehensive transcriptome sequencing. The overexpression of lncRNA MEG3 in H9C2 cells led to a significant reduction in cell proliferation, as evidenced by lower absorbance readings in the CCK-8 assay and reduced cell confluency in imaging analyses. However, flow cytometric analysis revealed no substantial differences in apoptosis between the lncRNA MEG3 overexpressing group and the control. Transcriptomic analyses demonstrated significant changes in gene expression and alternative splicing patterns, highlighting the intricate role of lncRNA MEG3 in cellular regulatory mechanisms. In conclusion, lncRNA MEG3 overexpression in rat cardiomyoblast H9C2 cells significantly inhibits cellular proliferation without markedly inducing apoptosis, suggesting a specific regulatory role in cellular growth processes. The transcriptomic alterations observed underscore the potential of lncRNA MEG3 as a key player in the molecular dynamics of cardiomyoblasts.
Chitinase from Bacillus sp. SRTI8: production, purification and biocontrol activities.
Sahnoun S, Bilal Yahiaoui, Aïcha Benlounissi … +2 more , Hassiba Laribi-Habchi, Abdenacer Mouffok
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054375 · Publisher ↗
From sand in the Algerian Sahara, an isolated strain of Bacillus called Bacillus sp. SRIT8 showed little chitinase activity when grown in a minimal medium supplemented with chitin (2.36 U). Using Plackett-Burman and Box-...
From sand in the Algerian Sahara, an isolated strain of Bacillus called Bacillus sp. SRIT8 showed little chitinase activity when grown in a minimal medium supplemented with chitin (2.36 U). Using Plackett-Burman and Box-Behnken statistical plans, we could maximize chitinase synthesis, which led to a notable increase in this enzymatic activity (112 U). The purification of the resulting enzyme involved three steps: ammonium sulfate precipitation, molecular exclusion chromatography, and anion exchange chromatography. This process yielded a specific activity of 5437.14 U/mg with a purification yield of 22.44%. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis examination revealed a protein band of about 31 kDa, and optimum enzyme activity was found at pH 5 and 40 °C. Enzyme activity was boosted by Ca+2, Na+, and Mn+2 ions but was suppressed by Hg+2 ions. The purified enzyme inhibited the growth of the plant pathogen Fusarium graminearum on wheat in both in vitro tests. So, it might prevent fungal infections in wheat throughout the germination process. The enzyme was also effective as a bioinsecticide, killing up to 52% of the larvae of Sitophilus granarius Linnaeus, an insect pest of stored grain. Our chitinase's capacity to hydrolyze fungus cell walls as well as insect cuticles can be utilised as biological control agent.
Bone morphogenetic protein signaling pathway: an essential role in intestinal homeostasis and diseases.
Cogo E, Guéraud F, Plaisancie P
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054374 · Publisher ↗
The bone morphogenetic protein (BMP) signaling pathway is a fundamental regulator of intestinal homeostasis, orchestrating the delicate balance between stem cell proliferation and epithelial differentiation along the cry...
The bone morphogenetic protein (BMP) signaling pathway is a fundamental regulator of intestinal homeostasis, orchestrating the delicate balance between stem cell proliferation and epithelial differentiation along the crypt-villus axis. In opposition to the Wnt signaling pathway, BMP signaling promotes epithelial maturation and inhibits excessive stem cell expansion, thereby ensuring proper renewal and functional integrity of the intestinal epithelium. Both epithelial and mesenchymal cell populations actively contribute to BMP signaling; mesenchymal cells serve as a primary source of BMP ligands and antagonists, while epithelial cells predominantly express BMP receptors and downstream effectors. This dynamic epithelial-mesenchymal dialogue establishes and maintains the intestinal stem cell niche and structural organization of the crypts. Dysregulation of BMP signaling has been increasingly implicated in the pathogenesis of inflammatory bowel diseases (IBD) such as Crohn's disease and ulcerative colitis, as well as in colorectal cancer (CRC). In inflammatory conditions, modulation of BMP ligands and antagonists influences epithelial regeneration and immune responses, highlighting their potential anti-inflammatory and anti-fibrotic roles. Conversely, in colorectal carcinogenesis, alterations in BMP pathway components-including mutations in BMP receptors and Smad effectors, alongside aberrant expression of BMP antagonists like Gremlin-1-disrupt the balance of intestinal homeostasis, promoting tumor initiation, progression, and metastatic potential via both canonical and non-canonical signaling mechanisms. This review comprehensively summarizes the current understanding of BMP signaling in intestinal physiology and pathology, emphasizing the critical interplay between epithelial and mesenchymal compartments, and the impact of the genetic context and pathway modulators. Further elucidation of BMP pathway dynamics promises novel therapeutic strategies for intestinal diseases through targeted modulation of this pivotal signaling cascade.
Biogenic synthesis and characterization of MgO nanoparticles from banana peel extract with evaluation of their antibacterial and antioxidant activities in cellular models.
Jawada EK, Mohammad HF, Jaffer KS
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054373 · Publisher ↗
The current research involved the preparation of Magnesium Oxide Nanoparticles (MgO NPs) by aqueous extraction of banana peels, then characterizing the resulting particles and incorporating them into polymer matrices to...
The current research involved the preparation of Magnesium Oxide Nanoparticles (MgO NPs) by aqueous extraction of banana peels, then characterizing the resulting particles and incorporating them into polymer matrices to provide antimicrobial activity for packaging materials. The resulting particles were characterized by ultraviolet-visible (UV-Vis) and scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and infrared (FTIR) spectroscopy. The ultraviolet spectrum of Magnesium Oxide nanoparticles showed sharp absorption peaks at 350 and 500 cm⁻¹. Both SEM and TEM revealed MgO NPs as almost spherical granular structures. XRD showed six main peaks of the crystalline mineral elements of the magnesium oxide nanoparticles. FTIR results confirmed that biologically active compounds act as reducing and capping agents for the nanoparticles. The magnesium oxide nanoparticles also showed antibacterial and antioxidant activity and non-toxicity.
Genetic polymorphisms and immune cytokine profiles in the cellular pathogenesis of polycystic ovary syndrome among Iraqi women.
Al-Taei OMS, Baqer Hussein Al-Harmooshee M
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054372 · Publisher ↗
Immune dysregulation and genetic polymorphisms are recognized as critical contributors to the pathogenesis of polycystic ovary syndrome (PCOS). The present study aimed to investigate the roles of specific cytokines and t...
Immune dysregulation and genetic polymorphisms are recognized as critical contributors to the pathogenesis of polycystic ovary syndrome (PCOS). The present study aimed to investigate the roles of specific cytokines and the CYP11A1 (rs4077582) polymorphism in Iraqi women diagnosed with PCOS. The current study included collecting samples from 100 women with PCOS and 100 healthy women as a control group. Clinical and laboratory tests were conducted at Diwaniyah General Hospital. The concentration of interferon-gamma, interleukin-2, interleukin-4 and interleukin-10 in the serum was determined utilizing the ELISA technique, while genetic CYP11A1(rs4077582) polymorphisms were determined using the PCR-RFLP technique. ELISA results demonstrated the concentration of interferon-gamma, interleukin-2 in FF in PCOS are significantly increased (123.8 ± 33.6, 45.77 ± 8.92 ng/ml respectively) compared with those in controls (23.5 ± 4.29, 7.31 ± 1.07 ng/ml respectively) while concentration of IL-4 and IL-10 reduced in patients (22.62 ± 6.24 and 5.81 ± 1.11 ng/ml respectively) compared to control (167.1 ± 18.62 and 37.54 ± 7.11ng/ml respectively). The results showed an increase in rate of mutant genotype CC and allele C in patients (34% and 64% respectively) compared to controls (12% and 25% respectively), while a decrease in the proportion of normal genotype TT and allele T in patients (6% and 36% respectively) compared with healthy subjects (62% and 75% respectively). immune function of IFN-γ, IL-2, IL-4 and IL-10 and CYP11A1(rs4077582) polymorphisms significantly associated with PCOS.
Inverse correlation of miR-196a and HOXB13 expression in Iraqi patients with prostate cancer.
Al-Khafaji HMA, Khadum RM, Ouda SM … +1 more , Mohammed MQ
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054371 · Publisher ↗
Prostate cancer (PCa), a significant health concern in aging men, is influenced by the HOXB13 gene, a key regulator of prostate development and cell differentiation. Dysregulation of HOXB13, including genetic variants an...
Prostate cancer (PCa), a significant health concern in aging men, is influenced by the HOXB13 gene, a key regulator of prostate development and cell differentiation. Dysregulation of HOXB13, including genetic variants and altered expression, is related to PCa risk and progression. Importantly, the expression of HOXB13 is modulated by microRNAs, particularly miR-196a. The miR-196a controls expression of genes via mRNA binding, causing degradation or inhibiting translation. It targets HOX genes, crucial for development, and exhibits variable activity in cancers, including PCa. Therefore, the interplay between PC, HOXB13, and miR-196a contributes to the understanding of the molecular basis of PCa and identifies potential therapeutic strategies. This study involved a case-control design, comprising 120 blood samples divided into 60 PCa patients and 60 controls. Molecular analyses were performed on these samples, involving total RNA extraction followed by purification via a commercial kit. Subsequently, complementary DNA was synthesized. A quantitative real-time polymerase chain reaction was used to measure the expression levels of the HOXB13 gene and miR-196a. Relative expression levels of both HOXB13 and miR-196a were determined using established quantification methodologies. Statistical analyses were conducted using SPSS and GraphPad Prism software. Our findings demonstrated a significant increase in HOXB13 gene expression (p≤0.01), specifically a fourfold elevation in PCa patients compared to healthy individuals. In contrast, miR-196a expression exhibited a significant decrease (p≤0.01), suggesting a potential inverse regulatory correlation with HOXB13. This study reveals a significant inverse correlation between HOXB13 and miR-196a expression in PCa patients. Specifically, the HOXB13 expression level was upregulated, while the miR-196a level was downregulated. These findings suggest that miR-196a may be used as a prospective tumor suppressor in PCa by negatively regulating HOXB13, thereby inhibiting cell proliferation and invasion. Consequently, miR-196a may emerge as a promising diagnostic molecular target for prostate cancer.
Molecular docking study and ADMET prediction of the effects of some food alkaloids on thyroxine homeostasis through their interactions with human thyroxine-binding globulin.
Maouche N, Lenchi N
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054370 · Publisher ↗
Thyroid hormones (THs) play a vital role in several physiological functions of the body. At the circulatory level, thyroxine (T4) has been found to be the predominant form of THs. The distribution of T4 in the blood is m...
Thyroid hormones (THs) play a vital role in several physiological functions of the body. At the circulatory level, thyroxine (T4) has been found to be the predominant form of THs. The distribution of T4 in the blood is mainly carried out by human thyroxine-binding globulin (hTBG). This process can be interfered with by various natural substances present in foods, particularly alkaloids. Some of these alkaloids have been shown to possess a cyclic chemical structure similar to that of T4. It has therefore been hypothesised that this class could potentially compete with T4 transport in the bloodstream. A molecular docking study and ADMET prediction were performed with ten selected food alkaloids. Predicted ADMET analysis revealed that all compounds tested had adequate solubility, high human gastrointestinal absorption and minimal risk of hepatotoxicity and cardiotoxicity. Molecular docking data showed that piperine, nigellidine, capsaicin, nigellicine, and 3-hydroxyquinine had a high affinity for hTBG, with respective binding energies of - 8.1, - 8.0, - 7.7, - 7.2 and - 7.1 kcal/mol. This finding indicates that these alkaloids were successfully positioned in the binding site of hTBG and had the ability to compete with T4 and increase its free level in the bloodstream. Therefore, two suggestions can be withdrawn, depending on the physiological state of the thyroid gland. Overconsumption of these alkaloids may lead to an imbalance in T4 homeostasis in both healthy individuals and hyperthyroid patients. Conversely, this competitive dynamic may offer a therapeutic advantage in the management of hypothyroidism.
Bitter frankincense water modulates hepatic enzyme activity in female patients with irritable bowel syndrome.
Khamaal Hussein Abod Al-Khafaji, Zahraa Mohammed Fakheir, Shaimaa Mohammed Ali Jasim
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054369 · Publisher ↗
This study aimed to determine and know the effect of bitter frankincense water on the levels of the functional liver enzymes in female patients with irritable bowel syndrome. This study was conducted in the consulting la...
This study aimed to determine and know the effect of bitter frankincense water on the levels of the functional liver enzymes in female patients with irritable bowel syndrome. This study was conducted in the consulting laboratories for pathological analyses in Iraq for the period from January to February 2025. The study was applied to 25 female patients, whose ages ranged from 20 to 65 years and healthy group consisted of 25 females, aged between 20 - 65. Bitter frankincense water, was bought from reputable local markets, enough to feed 25 female patients for two months. The amount was 3 ml/kg, and each female patient was given the dose according to his weight. This is done by dividing the dose in half, for the morning and for the evening. Before administering bitter frankincense water to female patients with irritable bowel syndrome, a five milliliter blood sample was obtained, and the liver enzymes were evaluated for the samples, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT). Subsequently, samples were collected from the same group of female patients with irritable bowel syndrome to compare the effects of bitter frankincense water before and after giving. The results of the study showed a significant decrease (P<0.05) in the levels of liver function enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT) in patients with irritable bowel syndrome in women after these patients took doses of FWB. The study's notable reduction in liver enzyme levels suggests that FWB may be a useful natural hepatoprotective treatment for people with IBS. These results encourage more investigation into FWB as a supplemental treatment for enhancing gut-liver axis balance and liver function in IBS patients.
Identification of novel variants with predicted pathogenicity as key targets in esophageal cancer.
Abbasi WA, Qureshi S, Qureshi MA … +1 more , Quraishy MS
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054368 · Publisher ↗
Esophageal cancer (EC) remains a major global health challenge due to its aggressive nature and poor prognosis. Genetic alterations play a crucial role in tumor progression; however, a deeper understanding of the genetic...
Esophageal cancer (EC) remains a major global health challenge due to its aggressive nature and poor prognosis. Genetic alterations play a crucial role in tumor progression; however, a deeper understanding of the genetic landscape of EC is essential for identifying novel and potent therapeutic targets. This study aims to identify key genes and their variants with potential pathogenicity driving EC progression. Whole-exome sequencing (WES) was performed on EC samples to identify missense variants. A comprehensive in-silico analysis was conducted using SIFT, FATHMM, PROVEAN, MutationTaster, and LRT to classify high-risk variants. Gene expression, mutation frequency, and prognostic relevance were analyzed using GEPIA and cBioPortal platforms. Protein stability was assessed with MuPro and I-Mutant to evaluate the impact of the identified variant, while protein-protein interaction (PPI) analysis via STRING and enrichment analysis through Metascape were performed to explore associated biological pathways. A total of 331 novel high-risk missense variants were identified across 274 genes and systematically refined, narrowing down to 23 prognostically significant variants in 11 genes (PSMC1, SCN8A, HNRNPA3, RPL23, COL5A2, TBL1XR1, TCP1, HNRNPD, CALM2, ABCC2, and HNRNPA1), which were also among the most differentially expressed in EC. Variants in these genes were predicted to destabilize their corresponding proteins, contributing to EC progression. In-silico survival analysis further indicated significantly worse outcomes for patients harboring alterations in these genes, including others. Protein stability analysis confirmed their destabilizing effects, while functional enrichment highlighted their involvement in key pathways driving tumorigenesis. This study identified 11 key DEGs harboring potentially pathogenic novel missense variants, highlighting vulnerabilities for precision-targeted therapies in EC.
Epigenetic and genetic events of oral squamous cell carcinoma: perspective on DNA methylation, silencing of tumor suppressor gene, and activating oncogenes.
Nizar Jawad Z
Cell Mol Biol (Noisy-le-grand) · 2025 Oct · PMID 41054367 · Publisher ↗
Oral squamous cell carcinoma (OSCC) is a major health burden in most parts of the world, and pathogenesis of the disease is strongly associated with a complex combination between genetic mutations and epigenetic alterati...
Oral squamous cell carcinoma (OSCC) is a major health burden in most parts of the world, and pathogenesis of the disease is strongly associated with a complex combination between genetic mutations and epigenetic alterations. The current study will explore the DNA methylation phenomenon and how it affects the silencing of tumour suppressor genes and activate oncogenes in OSCC in a bid to explain the driving molecular process behind tumour development. We evaluated 50 samples of the OSCC tissues with 50 of the adjacent normal tissues, evaluated DNA methylation patterns with methylation-specific PCR and quantitative reverse-transcriptase PCR measured the expression of the DNA repair genes like BRCA1 and MLH1, among others. This finding showed different levels of DNA methylation of cancerous and normal tissues with hypermethylation causing the inactivation of important tumor suppressor genes and hypomethylation causing the activation of oncogenes. In addition, the downregulation of DNA repair genes was noted to be highly significant in OSCC samples indicating that genomic-instability may be related to epigenetic changes. These results demonstrate that aberrant DNA methylation is central to OSCC growth and progression, thus helping in the future use of methylation patterns that can be used as early detection, diagnosis, and prognostic biomarkers. Our findings support the idea that genetic, along with epigenetic, profiling is an issue of key importance toward comprehending OSCC biology and personalized therapeutic interventions. It is recommended that further confirmation be carried out to verify the clinical significance of such epigenetic markers in bigger cohorts, as well as testing such markers in guided therapies, which might eventually lead to better patient outcomes in the management of oral cancer.
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