Cellular And Molecular Biology (Noisy-le-Grand, France)[JOURNAL]
Sun
200 papers
Analysis of the prospects of new therapeutic agents for the treatment of rheumatoid arthritis.
Blagov AV, Orekhov NA, Beloyartsev DF … +5 more , Churov AV, Kovyanova TI, Starodubtseva IA, Sukhorukov VN, Orekhov AN
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736299 · Publisher ↗
Rheumatoid arthritis (RA), a chronic autoimmune disease, is one of the major research themes in medicine. The current therapies have their limitations and cannot completely cure RA, but new therapeutic strategies are bei...
Rheumatoid arthritis (RA), a chronic autoimmune disease, is one of the major research themes in medicine. The current therapies have their limitations and cannot completely cure RA, but new therapeutic strategies are being proposed to reduce the shortcomings of approved drugs. This review will consider new potential treatment strategies for RA, including T-cell therapy, genetic editing and epigenetic regulation, what advantages and disadvantages they have and to what pathological target in RA they are directed.
Genotoxic of co-codamol for human lymphocyte culture in vitro.
Sada Jasim Abdulameer, Anfal Izaldeen Alkateeb, Layth Ammar Chyad Al-Shammari
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736298 · Publisher ↗
Co-codamol, a combination analgesic containing paracetamol and codeine phosphate, is widely used for pain relief, but its potential genotoxic effects on human lymphocytes remain largely unknown. This in vitro study inves...
Co-codamol, a combination analgesic containing paracetamol and codeine phosphate, is widely used for pain relief, but its potential genotoxic effects on human lymphocytes remain largely unknown. This in vitro study investigates the genotoxic potential of co-codamol on cultured human lymphocytes by assessing cell viability, mitotic index (MI), chromosomal aberration frequency, and micronucleus (MN) formation. Lymphocytes were exposed to varying concentrations of co-codamol (0.02-0.12 mg/mL), and cytotoxicity was determined using the MTT assay. Results showed that co-codamol significantly reduced cell viability in a dose-dependent manner, with complete cell death at 0.12 mg/mL. The mitotic index was significantly decreased at higher concentrations, and a statistically significant increase in chromosomal aberrations and micronucleus formation was observed in treated lymphocytes compared to the control group (p < 0.05). These findings provide important evidence that co-codamol exhibits genotoxic potential in vitro, suggesting a potential risk of DNA damage associated with its use. Further in vivo investigations are warranted to assess the clinical relevance of these genotoxic effects and to elucidate the underlying mechanisms of toxicity.
Protective effects of matrine on cardiomyocytes infected with coxsackievirus B₃ via modulation of the calpain-2/caspase-12 signaling pathway.
Sun Y, Mao Z, Liu J … +1 more , Geng Y
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736297 · Publisher ↗
Viral myocarditis (VMC) presents a substantial threat, especially for children, often leading to cardiogenic shock and fulminant myocarditis. Our study aimed to evaluate the role of calpain-2 and caspase-12, which were i...
Viral myocarditis (VMC) presents a substantial threat, especially for children, often leading to cardiogenic shock and fulminant myocarditis. Our study aimed to evaluate the role of calpain-2 and caspase-12, which were involved in the endoplasmic reticulum apoptosis pathway, and the influence of Matrine on these proteins during Coxsackie virus B₃ (CVB₃)-induced acute VMC mice in vitro and in vivo, shedding light on the potential cardioprotective effects. We first performed primary cultured cardiomyocytes, which were infected with CVB₃in vitro. We observed cell viability, the beating of cardiomyocytes and cytopathic effects. And we utilized Balb/c mice to establish the VMC animal model and determined viral titers, histopathological changes, and myocardial pathological scores. Furthermore, we detected CK-MB levels and myocardial cell apoptosis in vitro and in vivo. In order to further explore the possible mechanisms, the protein expression of calpain-2 (by immunohistochemistry and Western blot) and caspase-12 activity (by fluorescence assay for substrate cleavage) were detected in vitro and in vivo. Our findings indicated that, in comparison to the normal control group, the virus-infected group exhibited increased injured myocardial cells, virus titer, CK-MB levels, and apoptotic cells (P<0.05). Matrine treatment groups significantly reduced CK-MB levels, myocardial cellular damages and apoptosis in vitro and in vivo, with Matrine notably suppressing calpain-2 protein expression and Caspase-12 activity compared to the virus-infected group (P<0.05). In conclusion, our study revealed that calpain-2 and caspase-12 played roles in CVB₃-induced myocardial cell apoptosis. Matrine effectively mitigated myocardial cell injury and reduced apoptosis, thereby providing substantial protection against CVB₃infection in vitro and in vivo, which may be related to the down-regulation of calpain-2/caspase-12 signaling pathway.
Rutin treatment alleviates obesity-related aortic endothelium dysfunction in albino rats fed a high-fat diet.
Madkhali HA, Ganaie MA, Ansari MN … +7 more , Rehman NU, Hamad AM, Soliman GA, Alanazi KF, Ahmed MM, Hamadi AY, Alhawiti NM
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736296 · Publisher ↗
Flavonoids have recently been shown to be useful to people suffering from vascular disorders caused by a high-fat diet (HFD). The flavonoid rutin (RT) exhibits numerous pharmacological effects, including antioxidant, cyt...
Flavonoids have recently been shown to be useful to people suffering from vascular disorders caused by a high-fat diet (HFD). The flavonoid rutin (RT) exhibits numerous pharmacological effects, including antioxidant, cytoprotective, vasoprotective, and cardioprotective activities. The primary objective of this research was to assess the efficacy of RT against obesity-related vascular endothelial dysfunction (VED) in rats fed HFD. A total of 24 mature Wistar rats were blindly categorized into 4 treatment and control groups: normal control, obese control, and obese which were given RT at 50 and 100 mg/kg for the final 3 weeks of the experimental period. Animals' body mass and food consumption have been estimated periodically. In addition, liver mass and retroperitoneal fat mass per body mass, abdominal circumference (AC), LEE index, and body mass index (BMI) were estimated. Moreover, lipid profile parameters were assessed in serum. The effect on vascular endothelium reactivity was investigated in an isolated rat aorta. A histopathological investigation of the aorta was performed. The obese control group exhibited higher body, liver, and retroperitoneal fat weights. Significantly, RT intake reverses all these alterations. Furthermore, RT decreased food intake, AC, Lee index, and BMI in HFD-fed rats. The lipid profile of HFD-fed rats was also improved after RT treatment, with lower triglycerides, total cholesterol, LDL-C, and VLDL-C levels and higher HDL-C levels in the serum of HFD-fed rats. Through the ex-vivo investigation, RT groups showed improved vascular endothelium function in HFD-fed animals compared to the obese control group. Taking together, RT could be a promising option for preventing obesity-associated VED.
Low frequency of HER2 expression in colorectal cancer: A Tunisian single-center study.
Ben Lazreg K, Majdoub W, Bdioui A … +7 more , Krifa M, Lajmi Z, Belkacem O, Alaya M, Mestiri S, Hmissa S, Missaoui N
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736295 · Publisher ↗
HER2 expression is a potential theranostic and prognostic marker in some cancers, particularly in breast and gastric cancers. However, published data on HER2 expression in colorectal cancer (CRC) remain controversial. Th...
HER2 expression is a potential theranostic and prognostic marker in some cancers, particularly in breast and gastric cancers. However, published data on HER2 expression in colorectal cancer (CRC) remain controversial. This study investigates the immunohistochemical and molecular expression of HER2 in primary CRC and evaluates its clinicopathological and prognostic significance in Tunisian patients. A retrospective analysis was conducted on 144 CRC patients. HER2 status was assessed by immunohistochemistry and tissue microarray analysis, following the diagnostic criteria for gastroesophageal adenocarcinoma. CRC cases with ambiguous results underwent chromogenic in situ hybridization. The mean patient age was 61.9 years (male-to-female ratio: 1.18:1). Tumors were classified as colonic (74.3%) or rectal (25.7%), with 45.8% located in the left colon. Stage III disease was identified in 37.5% of cases, and distant metastases were present in 13.9%. HER2 expression results were as follows: negative (score 0/1+) in 142 cases (98.6%), equivocal (score 2+) in one case (0.7%), and overexpressed (score 3+) in one case (0.7%). No HER2 gene amplification was detected, and none of the metastatic CRC cases showed HER2 immunostaining. These findings suggest that HER2 overexpression and amplification in CRC are rarer than previously reported, highlighting the need for multicenter Tunisian studies to validate these results. The variability in HER2 immunostaining criteria further underscores the importance of a standardized scoring system to ensure consistency in both diagnosis and research.
Effects of abiotic factors on Zingiber officinale and Glycyrrhiza glabra to extract bioactive compounds under different time incubation and different salt concentrations.
I Almaghasla M, Khan T, Kanwal N … +1 more , M Al-Khayri J
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736294 · Publisher ↗
Zingiber officinale, commonly known as ginger, and Glycyrrhiza glabra, commonly known as licorice, are medicinal plants that are rich in bioactive compounds with various health benefits. This study aimed to investigate t...
Zingiber officinale, commonly known as ginger, and Glycyrrhiza glabra, commonly known as licorice, are medicinal plants that are rich in bioactive compounds with various health benefits. This study aimed to investigate the effects of different growth durations and salt concentrations on the production of bioactive compounds by these plants. This experiment was conducted under natural conditions and the plants were subjected to salt stress at different stages of growth. This analysis focused on assessing the production of polysaccharides, flavonoids, ergosterol, adenine, adenosine, hypoxanthine, and guanosine by UV spectrophotometer and high-performance liquid chromatography (HPLC) in both plants. The results showed that Z. officinale exhibited the highest polysaccharide content at 20-d of growth with 3mM salt, whereas G. glabra showed slightly lower polysaccharide content. Similarly, Z. officinale had higher flavonoid content at 25-d of growth with 5 mM salt compared to G. glabra. Additionally, Z. officinale demonstrated higher concentrations of ergosterol, adenine, adenosine, hypoxanthine, and guanosine than G. glabra, particularly at 25-d of growth with 5 mM salt. This study provides valuable insights into the production of bioactive compounds in Z. officinale and G. glabra under different growth conditions, which can be beneficial for optimizing their cultivation and utilization in various applications including pharmaceuticals and functional foods.
Anti-inflammatory effect of thyme on rheumatoid arthritis in animal model.
Blbas SSS, Hiwa Ramadhan Fatah, Safia Sabr Ibrahim Blbas
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736293 · Publisher ↗
Thyme (Thymus vulgaris) is a Mediterranean herb known for its culinary, cosmetic, and medicinal applications as it has been discovered that the plant has many clinical properties such as anti-inflammatory, anti-microbial...
Thyme (Thymus vulgaris) is a Mediterranean herb known for its culinary, cosmetic, and medicinal applications as it has been discovered that the plant has many clinical properties such as anti-inflammatory, anti-microbial, and antioxidant properties. Sandwich Elisa technique was used to determine the concentration of cytokines. Phenolic contents and other active compounds of thyme were analyzed by Gas Chromatography-Mass Spectrometry (GC-Mass). In this study 15 male adult albino rats were divided into 3 groups (n=5), group one (G1) was the control group which fed on basal diet. Group two (G2) was the Rheumatoid arthritis (RA) group in which the rats were inoculated with 0.1 ml of CFA (Complete Freund's Adjuvant) yet fed on basal diet. Group three (G3) was the treatment group in which rats were inoculated with CFA along with the administration of thyme extract orally for 22 days. The results show that treatment with thyme extract significantly reduced proinflammatory cytokines IL-1 (interleukin-1), IL-6 (interleukin-6) and TNF- α (tumor-necrosis factor-alpha). Anti-inflammatory IL-10 (interleukin-10) showed a significant increase in the thyme-treated group. CD4 T (cluster of differentiation-4) cell levels showed a significant difference, while sCD14 (soluble cluster of differentiation-14) levels were non-significant in the thyme group compared to the RA group. Inflammatory markers (C-reactive proteins) CRP and (anti-cyclic citrullinated peptide) Anti-CCP antibodies were both significantly elevated in RA and significantly reduced by thyme treatment. Although body weight changes were statistically non-significant, they were visibly prominent. Paw edema was significantly decreased in the thyme-treated group. (Matrix-metalloproteinase) MMP-1 levels and neutrophil counts were both elevated in RA and significantly reduced following thyme extract treatment.
The use of monoclonal antibodies for the treatment of atherosclerosis: current status and prospects.
Blagov AV, Pleshko EM, Maltseva ON … +3 more , Asoyan AZ, Ravani AL, Orekhov AN
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736292 · Publisher ↗
Atherosclerosis remains a leading cause of cardiovascular morbidity and mortality worldwide, underlying major conditions such as coronary heart disease and stroke. The pathogenesis of atherosclerosis is tightly linked to...
Atherosclerosis remains a leading cause of cardiovascular morbidity and mortality worldwide, underlying major conditions such as coronary heart disease and stroke. The pathogenesis of atherosclerosis is tightly linked to chronic inflammation and dysregulated lipid metabolism, processes that are also implicated in other inflammatory diseases like rheumatoid arthritis and psoriasis. Monoclonal antibodies (mAbs) have emerged as a promising therapeutic strategy, offering targeted intervention against key molecular drivers of atherosclerosis. This review summarizes recent advances in the development and clinical application of mAbs targeting both lipid-lowering pathways-such as low-density lipoprotein (LDL) and proprotein convertase subtilisin/kexin type 9 (PCSK9)-and inflammatory mediators, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-17 (IL-17). Notably, anti-PCSK9 antibodies like alirocumab and evolocumab have demonstrated significant reductions in LDL-C levels and cardiovascular events in large-scale clinical trials. Similarly, antibodies targeting inflammatory cytokines have shown efficacy in reducing vascular inflammation and associated risks. The review also discusses the advantages and limitations of therapeutic mAbs, such as their high specificity, potential for adverse immune responses, and challenges related to tissue penetration and cost. Overall, monoclonal antibody therapy represents a significant advancement in the management of atherosclerosis, with ongoing research aimed at optimizing efficacy, safety, and accessibility. Future directions include the development of novel mAbs and combination therapies to further improve cardiovascular outcomes in patients with atherosclerotic disease.
LINC01232 regulates miR-516a-5p/BCL9 axis to promote triple-negative breast cancer progression.
Liu W, Niu Y, An J
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736291 · Publisher ↗
Triple-negative breast cancer (TNBC) is characterised by an absence of the oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), for which there are few therapeutic opti...
Triple-negative breast cancer (TNBC) is characterised by an absence of the oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), for which there are few therapeutic options and the prognosis is poor. This research sought to explore the particular function of the long non-coding RNA (lncRNA) LINC01232 in TNBC and its regulatory impacts on the miR-516a-5p/BCL9 pathway. In this study, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression level of LINC01232 in TNBC tissues. We also examined its regulatory influences on miR-516a-5p and BCL9 via cellular function tests and a luciferase reporter experiment. Evaluated the effect of LINC01232 silencing on proliferation, migration and invasion of breast cancer cells. Results showed that LINC01232 expression was abnormally high in TNBC tissues in comparison to normal tissues. Inhibition of LINC01232 expression markedly impeded breast cancer cell proliferation, clone formation, migration and invasion. We found that LINC01232 competes with miR-516a-5p for binding, thereby reducing its expression and subsequently increasing BCL9 expression. In conclusion, our results indicate that LINC01232 facilitates the malignant development of TNBC through the miR-516a-5p/BCL9 pathway, providing fresh perspectives on the pathogenesis of TNBC and pinpointing potential therapeutic targets.
Molecular mechanisms underlying the antitumor activity of human and bovine alpha-lactalbumin-oleic acid complexes in a murine mammary adenocarcinoma model.
Al-Taee MM
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736290 · Publisher ↗
The alpha-lactalbumin-oleic acid complexes (BAMLET and HAMLET) derivatives have shown remarkable anticancer capabilities in various preclinical studies with potential applications in oncology. The current study investiga...
The alpha-lactalbumin-oleic acid complexes (BAMLET and HAMLET) derivatives have shown remarkable anticancer capabilities in various preclinical studies with potential applications in oncology. The current study investigates the anti-cancer activity of synthesized human alpha-lactalbumin oleic acid (HAMLET) and bovine alpha-lactalbumin oleic acid (BAMLET) complexes on AN3 mouse mammary adenocarcinoma cancer cell line, a long-standing model for cancer research. We investigated multiple therapeutic endpoints including tumor volume reduction, survival rates, histopathological changes, as well as the molecular mechanisms allying the treatment response. A significant suppression of tumor growth was observed in both groups treated with HAMLET and BAMLET when compared with the control group, with HAMLET showing slightly better effectiveness in tumor growth inhibition. Histological examination revealed tumor necrosis, apoptosis, and decreased cell proliferation in treated mice, cancer cells were dying and also growth architecture was disrupted which indicates that both complexes cancer cell death. Thus, we investigated major molecular pathways associated with the anticancer activity of these compounds. Findings revealed the activation of supportive apoptotic pathways alongside downregulation of fundamental oncogenes linked to growth endurance and metastasis. Likewise, the safe response was augmented in the treated groups as shown by greater infiltration of immune cells into the tumor microenvironment. Survival rates were significantly higher in the HAMLET and BAMLET treatment groups compared to control, suggesting that these assemblies may prolong survival by effectively reducing cancer burden. The results highlight once again the exceptional breast cancer treatment efficacy of the alpha-lactalbumin-oleic acid complex.
Phytochemical screening and evaluation of antibacterial and antifungal activities of Callistemon viminalis cultivated in Iraq.
Zaineb Aziz Ali, Fatimah Ahmed Challoob Al-Khuzaee, Yasser Kadhim Hashem Al-Zwaini … +5 more , Mustafa Mohammed Albassam, Rwaieda Adil Muhsen, Balqess Hisham Salih, Mohammed Hasan Kadhim, Sumia Samer Tayeh
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736289 · Publisher ↗
Callistemon viminalis, a member of the Myrtaceae family, is traditionally used to treat infections, respiratory, and gastrointestinal disorders. This study aimed to investigate the phytochemical profile and evaluate the...
Callistemon viminalis, a member of the Myrtaceae family, is traditionally used to treat infections, respiratory, and gastrointestinal disorders. This study aimed to investigate the phytochemical profile and evaluate the antibacterial and antifungal activities of C. viminalis leaves cultivated in Iraq. Leaves were collected, dried, and extracted sequentially with hexane and 70% ethanol. Preliminary phytochemical screening revealed the presence of saponins, terpenoids, alkaloids, and flavonoids. The antimicrobial activity of the ethanol extract was assessed using the agar well diffusion method against three Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus sp.), two Gram-negative bacteria (Escherichia coli, Klebsiella sp.), and one fungal strain (Candida albicans). The ethanol extract exhibited notable inhibitory effects, particularly against Gram-positive bacteria and C. albicans, with activity increasing in a concentration-dependent manner. GC-MS analysis of the hexane extract identified key bioactive compounds, including beta-sitosterol and vitamin E. These findings highlight the significant pharmacological potential of C. viminalis leaves and support their traditional use as a source of natural antimicrobial agents. Further studies are recommended to isolate and characterize the active constituents responsible for these effects.
Impact of smoking and environmental toxins on diabetic retinopathy: role of trace elements, lipid profiles, and vitamin A.
Alrikabi NH, Chemingui H, Soheir N Abd El-Rahman … +1 more , Madiha Kamoun
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736288 · Publisher ↗
Diabetic retinopathy (DR), a leading cause of adult blindness, is influenced by physiological factors such as lipid profiles, vitamin A, and trace elements, as well as environmental factors like smoking. This study inves...
Diabetic retinopathy (DR), a leading cause of adult blindness, is influenced by physiological factors such as lipid profiles, vitamin A, and trace elements, as well as environmental factors like smoking. This study investigated the relationship between HbA1C, glucose levels, trace elements (lead, zinc, selenium, magnesium), vitamin A, and lipid profiles (TC, TG, HDL, LDL, VLDL) in type 2 diabetes (T2D) and DR patients, considering the impact of smoking. The study, conducted at Nasiriya General Hospital and the Center for Endocrinology and Diabetes (May 2023-March 2024), included three age groups (15-35, 35-55, and 55-75 years) divided into smokers and non-smokers. Results showed significantly higher glucose, HbA1C, lipid and selenium levels in T2D and DR patients compared to controls (p≤0.05), with smokers exhibiting greater lead levels. Zinc and vitamin A were significantly lower in DR patients, particularly among smokers. The findings highlight smoking as a source of lead and other toxins that exacerbate DR and diabetes-related complications, emphasizing the critical link between environmental health and chronic disease management.
Sequencing of TNFα and IFNϒ genes associated with Toxoplasma gondii infection among Erbil residents-Iraq.
Ahmed Akil Khudhair Al-Daoody
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736287 · Publisher ↗
Toxoplasmosis is a serious disease that affects all age groups and may even threaten the lives of some people. Fifty serum samples were taken from patients has Toxoplasmosis who attended Rizgary Teaching Hospital in Erbi...
Toxoplasmosis is a serious disease that affects all age groups and may even threaten the lives of some people. Fifty serum samples were taken from patients has Toxoplasmosis who attended Rizgary Teaching Hospital in Erbil City, and 38 serum samples were taken from healthy individuals as a control group, with ages between 18-60 years old, from the period January 2024 to March 2025. The purpose of the research is to determine the gene sequence of TNFα and IFNϒ associated with Toxoplasmosis. The results showed that the infection prevalence rate in the age group (18-27) years was 17 (56.7%) in comparison to healthy individuals 13 (43.3%), and in the age group (28-37) years was 20 (62.5%) compared to healthy control 12 (37.5%), and in the age group (38-47) years was 10 (76.9%) in comparison to the controls 3 (23.1%), while in the age group (≥47) years was 3 (23.1%) compared to the controls 10 (76.9%) with significant variations (P=0.03). The prevalence rate of Toxoplasmosis in males was 17 (48.6%) in comparison to the healthy controls 18 (51.4%), while in females it was 33 (62.3%) compared to the healthy individuals 18 (51.4%), with no significant variations (P=0.29). The mean level of Toxoplasmosis IgM was (2.28±0.18) when compared to healthy group (0.12±0.03), and the mean level of Toxoplasmosis IgG was (2.12±0.18) when compared to healthy control (0.09±0.02). The mean level of TNF-α (pg/ml) was (10.34±0.39) in comparison to the control group (4.89±0.31), and the mean level of INF-γ (pg/ml) was (10.72±0.36) in comparison to the controls (4.80±0.29) with highly significant variations (P=0.001). Moreover, direct correlation between Toxoplasma IgM with IgG was (r=.568), with TNF-α was (r=.607), with INF-γ was (r=.528), with highly significant variations P= (.000, .000, .000) respectively. Also, there were direct correlations between Toxoplasma IgG with IgM (r=1), with TNF-α (r=.550), with INF-γ (r=.576), with highly significant variations P= (.000, .000, .000) respectively. The TNFα gene sequence ID 7132 of rs767455 in the position AA was changed to AG and GG, respectively, in samples 1-10 in comparison to the control group. Also, the INFϒ gene sequence ID 3458 of rs2430561 in the position TT was changed to TA and AA, respectively, in samples 1-10 in comparison to the control group.
Molecular evaluation of quercetin effects in a murine model of giant cell tumor of bone: an in vivo pilot study.
Monroy-Quiroz DL, Luna-Angulo AB, Galicia-Canales BE … +9 more , Sánchez-Chapul L, Hernández-González O, Aguilar-Gaytán MDR, Santamaría-Olmedo MG, Hidalgo-Bravo A, Couder-García BDC, Lara-Hernández G, Estrada-Villaseñor EG, Landa-Solís C
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40736286 · Publisher ↗
Quercetin, a flavonoid derived from plant sources, has been extensively studied for its numerous biological properties, particularly its potential antitumor action against various malignant neoplasms. In our experience w...
Quercetin, a flavonoid derived from plant sources, has been extensively studied for its numerous biological properties, particularly its potential antitumor action against various malignant neoplasms. In our experience with a giant cell tumor of bone cell line (TIB-223), we demonstrated that quercetin has the ability to induce apoptosis via caspase-3. Therefore, this study aimed to evaluate molecular markers for apoptosis, necrosis, and cell proliferation in a murine model of giant cell tumor of bone, to determine whether the behavior reported for quercetin in 2D remains consistent in a 3D in vivo tumor model. Tumor constructs based on TIB-223 cells were implanted into athymic mice, and two weeks post-implantation, the mice were orally administered quercetin at a concentration of 100 mg/kg body weight once a day for two weeks. The control group received only 200 µL of the vehicle. Our results demonstrate the activation of two cell death pathways in the implanted tumors: apoptosis, via Caspase-8 to Caspase-3 activation, and necroptosis, via RIPK1. No significant effect on cell proliferation was observed, as PCNA expression remained unchanged. Our results suggest that quercetin may induce specific mechanisms of cell death without significantly altering cell proliferation in the tumor model induced in mice.
Integrated genomic and molecular insights into astrocyte- and oligodendrocyte-derived amyotrophic lateral sclerosis: focus on miRNAs and extracellular vesicles.
Asghari Jafari E, Arabi M, Bereimipour A
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40618341 · Publisher ↗
Motor neurons in the brain and spinal cord begin to die off in Amyotrophic lateral sclerosis (ALS), a disease that can be fatal. Molecular pathways in neurological disease, especially ALS, remain a challenge in the medic...
Motor neurons in the brain and spinal cord begin to die off in Amyotrophic lateral sclerosis (ALS), a disease that can be fatal. Molecular pathways in neurological disease, especially ALS, remain a challenge in the medical sciences. In this disease, a disorder in both astrocytes and oligodendrocytes can cause the disease to progress. This study aimed to investigate the molecular mechanisms and find key elements between these two cells in ALS with a bioinformatics perspective. In this study, using integrated and continuous bioinformatics analytics by various tools and databases, we investigated genes, protein products, and miRNAs between astrocytes and oligodendrocytes. The obtained data were involved in the Cellular senescence, actin cytoskeleton, and cell cycle signaling pathways. Then, after careful evaluation of the information, TP53, MDM2, KRAS, PTPRC, and GSK proteins were candidates, which are regulated by hsa-miR-564, hsa-miR-496-5p, hsa-miR-324-5p, hsa-miR-296-5p, and hsa-miR-4258-3p miRNAs. Finally, the four genes had a more robust and better relationship in this study between astrocyte and oligodendrocyte-derived ALS.
Molecular and cellular effects of chronic low-dose X-ray exposure on thyroid function and blood cell parameters in radiology staff.
Mahmood SQ, Talabany BK, Hama-Soor TA
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40618340 · Publisher ↗
This study investigated the impact of long-term, low-dose occupational exposure to X-rays on thyroid function and hematological parameters in radiology staff. A cross-sectional analysis was conducted on 136 radiology and...
This study investigated the impact of long-term, low-dose occupational exposure to X-rays on thyroid function and hematological parameters in radiology staff. A cross-sectional analysis was conducted on 136 radiology and radiation-related employees. Thyroid status, assessed via ultrasonography and serum T3, T4, and TSH measurements, was compared with complete blood counts (CBC). While no significant gender-based differences in thyroid hormones or structure were observed, TSH levels correlated significantly with occupational group (P=0.016), and T3 levels correlated with working hours (P=0.03). Radiologists exhibited higher RDW-CV compared to radiographers and other staff (P=0.009). Significant gender differences were noted in lymphocyte counts, hemoglobin, red blood cells, and hematocrit. In males, WBC and MCHC fluctuated significantly with increased working hours. These findings suggest that chronic, low-dose X-ray exposure may influence thyroid hormone regulation and hematopoiesis in radiology professionals. Further research is warranted to elucidate the underlying cellular and molecular mechanisms and to refine radiation safety protocols.
Association of inflammatory gene variants with problematic alcohol use in a Colombian population.
Rey Buitrago M, Aristizabal Gutierrez FA
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40618339 · Publisher ↗
Alcohol dependence is a multifactorial disease that constitutes a significant public health concern and a significant risk for individual, family, and social health. Its genetic component exhibits significant ethnic vari...
Alcohol dependence is a multifactorial disease that constitutes a significant public health concern and a significant risk for individual, family, and social health. Its genetic component exhibits significant ethnic variation and is closely associated with the personal evolution of the disease. However, although multiple loci have been identified, no functional variants have been identified. In this work, we selected some genes from the inflammatory response pathway and searched for SNV (single nucleotide variants) in their promoter region that could be associated with the disease. We compared cases of problematic alcohol consumption (n=66) with controls (n=73) in a population sample taken at the National University of Colombia, Bogotá headquarters. Peripheral blood DNA extraction was performed. We used PCR and Sanger sequencing to find 28 SNVs and one STR in 10 inflammatory response genes that are connected to alcoholism. Then, using various bioinformatic tools, the analysis of haplotypes, linkage disequilibrium, epistasis and genetic networks was carried out. Allele and genotypic frequencies for this Colombian population were reported for the first time. Additionally, we found haplotypes that could be protective and risk factors for the disease, and gene interactions that have cumulative effects related to the drinker phenotype. The investigation of haplotypes, gene interaction, and gene networks is a highly effective methodology for identifying potential associations in small samples. Additionally, SNCA, IL-6R1, TNFR1, and MIF genes were profiled for further studies.
Anti-Müllerian hormone as a diagnostic and prognostic marker in polycystic ovary syndrome: a clinical study.
Al-Jawadi ZAM, Abbas İA
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40618338 · Publisher ↗
A study was conducted on anti-Müllerian hormone (AMH) and polycystic ovary syndrome (PCOS) in women. Samples were collected from 96 women with PCOS and 91 control women, aged 20 to 45 years for both groups. Levels of AMH...
A study was conducted on anti-Müllerian hormone (AMH) and polycystic ovary syndrome (PCOS) in women. Samples were collected from 96 women with PCOS and 91 control women, aged 20 to 45 years for both groups. Levels of AMH, estrogen, progesterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), LH/FSH ratio, endometrial growth rate (WHR), and body mass index (BMI) were measured. The results showed significantly lower AMH levels in women with PCOS compared to healthy women at the probability level (P=0.05). The results also demonstrated a significant positive association between the prevalence of PCOS and AMH levels for estrogen, LH, and LH/FSH. It had an inverse relationship with AMH, progesterone, FSH, endometrial growth rate (WHR), and body mass index (BMI). Furthermore, the risk of AMH deficiency in women with PCOS increases with age, due to decreased fertility and egg production from the ovaries, especially after the age of 30, as well as weight gain. This suggests that age-related declines in AMH concentrations and weight gain are indicative of increased risk factors for PCOS. Finally, this study demonstrated a relationship between PCOS risk factors and AMH concentrations, suggesting that low AMH concentrations increase the risk of PCOS, especially with age. This suggests the potential for incorporating AMH into early detection tests and the development of more effective treatments for this condition.
Functional analysis of intron 3 in the regulation of gene expression of the human lipoprotein lipase gene.
Sabri NH, Bastaki NK, Al-Bustan SA
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40618337 · Publisher ↗
Lipoprotein lipase (LPL) is a key enzyme that hydrolyzes triglycerides (TGs) into free fatty acids. Several genetic variants of LPL are directly or indirectly associated with variations in lipid levels, causing different...
Lipoprotein lipase (LPL) is a key enzyme that hydrolyzes triglycerides (TGs) into free fatty acids. Several genetic variants of LPL are directly or indirectly associated with variations in lipid levels, causing different lipid metabolic disorders. Previous studies on the LPL gene have shown that exons and introns are essential for gene expression and regulation. However, mechanisms through which introns regulate gene expression and function remain unclear. In this study, we successfully designed a protocol to assess the function of LPL intron 3 in LPL regulation. This was accomplished by constructing luciferase reporter vectors, containing full and partial intron 3 fragments from a healthy human DNA sample. These recombinant constructs facilitated the analysis of transcriptional activity using dual-luciferase reporter assays in cell lines. The results showed that the luciferase activity of the chimeric firefly luciferase reporter construct containing the full-length LPL intron 3 was higher than that of other constructs. In this study, a successful protocol was developed to assess the function of LPL intron 3 in regulation of the LPL gene. This protocol provides a novel method for functional analysis of introns and intronic variants that can be applied to other genes.
CTHRC1 overexpression in gastric cancer patients contributes to a poor prognosis and is related to immune cell infiltration.
Tao Guo, Qixin Xie, Hon Deng … +2 more , Changjun Yu, Changyi Fang
Cell Mol Biol (Noisy-le-grand) · 2025 Jul · PMID 40618336 · Publisher ↗
Gastric cancer is a common solid tumor of the digestive system, This research aimed to investigate the relationships among CTHRC1 expression, prognostic values and tumor-infiltrating immune cells (TIICs) in GC patients.T...
Gastric cancer is a common solid tumor of the digestive system, This research aimed to investigate the relationships among CTHRC1 expression, prognostic values and tumor-infiltrating immune cells (TIICs) in GC patients.The expression of CTHRC1 in gastric cancer patients was analyzed using the GEPIA database and the TCGA database. The relationship between CTHRC1 expression and survival of gastric cancer patients was then explored using the Kaplan-Meier Plotter database and TCGA database.Subsequently, we explored whether there was an associative effect between the expression of CTHRC1 and TIICs in gastric cancer tissues. Then we constructed a prognostic model using immunomodulatory genes related to CTHRC1 and verified the specificity and accuracy of the model.Compared with normal tissues, the expression of CTHRC1 was significantly upregulated in gastric cancer tissues. And the high expression of CTHRC1 was associated with poor prognosis of gastric cancer patients.It is suggested that CTHRC1 is a reliable prognostic indicator for predicting the prognosis of gastric cancer patients.Then we successively used univariate and multivariate COX methods to obtain three high-risk immunomodulatory genes (TGFB2, CXCR4 and NT5E) and one low-risk immunomodulatory gene (TNFRSF18) associated with CTHRC1. These four immunomodulatory genes were used to construct a prognostic model for gastric cancer.Gastric cancer patients with high CTHRC1 expression have a poor prognosis and are associated with immune cell infiltration. Therefore, CTHRC1 can be considered as a potentially reliable prognostic indicator for gastric cancer patients.
About
- Frequency
- Sun
- Papers found
- 200
- RSS feed
- Subscribe