Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia[JOURNAL]
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200 papers
[Pharmacogenomics of cisplatin-based chemotherapy in ovarian cancer patients from Yakutia].
Кhokhrin DV, Khrunin AV, Ivanova FG … +3 more , Moiseev AA, Gorbunova VA, Limborskaia SA
Mol Gen Mikrobiol Virusol · 2013 · PMID 24645271
DNA polymorphism is an important component of the interindividual variation in reactions of patients to the same drugs. In this work, evaluation of the association between polymorphisms in 106 genes involved in key proce...
DNA polymorphism is an important component of the interindividual variation in reactions of patients to the same drugs. In this work, evaluation of the association between polymorphisms in 106 genes involved in key processes of cellular activity (xenobiotic metabolism, DNA repair, the cell cycle, and apoptosis), and outcomes in a cohort of Yakut ovarian cancer patients receiving cisplatin-based chemotherapy was carried out. The polymorphism in the CDKN1B gene (rs34330) was found to be associated with complete tumor response and progression-free survival. SNPs in EPXH1 gene (rs2234922 and rs2260863) were correlated with hearing impairment. A SNP in NBN gene (rs1063045) was associated with severe emesis.
[Association of the polymorphism of dna repair genes with chromosomal aberrations in lung cancer patients].
Bakanova ML, Minina VI, Savchenko IaA … +4 more , Timofeeva AA, Dudkina OA, Titov VA, Verzhbitskaia NE
Mol Gen Mikrobiol Virusol · 2013 · PMID 24645270
The results of the examination of association of polymorphisms of DNA repair genes and chromosomal aberrations in lung cancer patients are discussed. A significant positive association between the hOGG1 G/G genotypes, XP...
The results of the examination of association of polymorphisms of DNA repair genes and chromosomal aberrations in lung cancer patients are discussed. A significant positive association between the hOGG1 G/G genotypes, XPD G/G genotype and lung cancer was found. The hOGG1 C/C genotypes were significantly negatively associated with lung cancer. The patient chromosomal aberration frequencies were significantly higher than in control. Carriers of all APE1 and XPD genotypes, XRCC1 G/G genotype, ADPRT T/T genotype, hOGG1 C/C and Ser/Cys genotypes had statistically significant differences in the level of the chromosomal aberrations between patient and control groups. Statistically significant differences in the level of chromosomal aberrations between XPD T/T and G/G genotype of lung cancer patients were observed.
[Genetic diversity of group A rotavirus isolates found in Western Siberia in 2007-2011].
Zhirakovskaia EV, Aksanova RKh, Gorbunova MG … +5 more , Tikunov AIu, Kuril'shchikov AM, Sokolov SN, Netesov SV, Tikunova NV
Mol Gen Mikrobiol Virusol · 2012 · PMID 23248851
Genetic analysis of group A rotavirus recovered from fecal samples of children admitted to hospitals in Novosibirsk and Omsk during four epidemic seasons 2007, 2007/2008, 2009/2010, 2010/2011 was performed. A total of 14...
Genetic analysis of group A rotavirus recovered from fecal samples of children admitted to hospitals in Novosibirsk and Omsk during four epidemic seasons 2007, 2007/2008, 2009/2010, 2010/2011 was performed. A total of 1416 rotavirus isolates were genotyped using multiplex PCR. The isolates of the most common rotavirus genotypes G1P[8], G4P[8], G2P[4], G3P[8] co-circulated in Western Siberia during 2007-2011. In isolated cases G9P[8], G2P[8], G3P[9], and G4P[6] genotypes were detected. Change of dominant genotype from G1P[8] to G4P[8] occurred in 2008 in Omsk and in Novosibirsk in 2009 as well. Incidence and distribution of rotavirus genotypes differed and changed every epidemic season in both cities. The phylogenetic analysis based on VP4 (VP8*), VP7, and VP6 gene sequences showed that the majority of isolates from Novosibirsk and Omsk were clustered together and demonstrated high level homology with rotavirus isolates found in other regions of Eurasia. In addition, a rare P[8]b (OP354-like) subtype of the VP4 gene was identified in fourteen isolates (G9, G1, and G4) in Novosibirsk and in a single isolate Omsk08-381/G9P[8]b in Omsk. The results obtained in this study demonstrate the necessity of long-term monitoring of rotavirus isolates in Western Siberia. This is important for selection of rotavirus vaccine for immunization of infants, improvement of diagnostic kits and understanding of the epidemiology and the evolution of group A rotaviruses.
[Molecular-genetic characterization of canine and rangiferine brucella isolates from different regions of Russia].
Kulakov IuK, Tsirel'son LE, Zheludkov MM
Mol Gen Mikrobiol Virusol · 2012 · PMID 23248850
GOAL: Comparative molecular-genetic characterization of Brucella isolates from dogs and reindeers in Russia by molecular-genetic typing methods. MATERIALS AND METHODS: 19 canine and 2 rangiferine Brucella isolates were s...
GOAL: Comparative molecular-genetic characterization of Brucella isolates from dogs and reindeers in Russia by molecular-genetic typing methods. MATERIALS AND METHODS: 19 canine and 2 rangiferine Brucella isolates were studied by molecular typing methods based on PCR for differential species and biovar specific molecular targets and MLVA (multiple locus variable number tandem repeats analysis) using primers to 12 known variable loci. RESULTS: Using PCR for differential molecular targets, canine Brucella isolates were characterized as B. canis and rangiferine isolates as B. suis biovar 4. MLVA revealed 5 identical and 7 variable MLVA loci. Using the dendrogram. all the isolates on the data of 12 loci were classified into the close related cluster. On the other hand, high discrimination power of MLVA with a resulting Hunter and Gaston discriminatory index (HGDI) of 0.9842 was shown to reveal genetic diversity for the isolates of 17 MLVA genotypes. CONCLUSION: B. canis and B. suis isolates from different geographical regions in Russia were genetically close related, thereby confirming known genetic relationship between these species. Related MLVA genotypes of isolates were connected to certain regions of preliminary isolation in Russia. To improve the system ofbrucellosis surveillance in Russia MLVA typing of more canine and rangiferine Brucella isolates having epidemiological danger for humans is required to be studied.
[Genetic determinancy of the change in the VE-cadherin expression and intensified vessel deendothelisation at hemorrhagic fever with renal syndrome].
Baĭgil'dina AA, Islamgulov DV
Mol Gen Mikrobiol Virusol · 2012 · PMID 23248849
The goal of this work was to determine a correlation between the VE-cadherin and circulating endothelial cells (CECs) blood levels at hemorrhagic fever with renal syndrome (HFRS) of different severity and research associ...
The goal of this work was to determine a correlation between the VE-cadherin and circulating endothelial cells (CECs) blood levels at hemorrhagic fever with renal syndrome (HFRS) of different severity and research association between the VE-cadherin gene c. 1550T>C missense mutation and HRFS severity. Significant decreasing of the VE-cadherin and increasing of CECs blood levels in the course of the disease in all studied groups was established. Most prominent changes were found at severe type with complications. There was found a strong negative correlation between these two indexes. There was significant high frequency of homozygotic genotype *T/*T at severe type with complications. It was concluded that there was increased endothelium desquamation due to the VE-cadherin internalization at moderate and severe uncomplicated types of HFRS and as a result ofVE-cadherin gene c. 1550T>C missense mutation at severe type with complications.
[Molecular characterization of the multidrug-resistant Acinetobacter baumannii strains and assessment of their sensitivity to the phage AP22].
Popova AV, Miakinina VP, Platonov ME … +1 more , Volozhantsev NV
Mol Gen Mikrobiol Virusol · 2012 · PMID 23248848
The molecular analysis of 130 multidrug-resistant nosocomial Acinetobacter baumannii strains was performed. The strains were obtained from patients admitted to different Russian hospitals (Chelyabinsk, Moscow, Nizhni Nov...
The molecular analysis of 130 multidrug-resistant nosocomial Acinetobacter baumannii strains was performed. The strains were obtained from patients admitted to different Russian hospitals (Chelyabinsk, Moscow, Nizhni Novgorod, and St. Petersburg) in 2005-2010. Species identification was performed using the amplified 16S rRNA gene restriction analysis and by determining intrinsic for A. baumannii blaQXA-51-like genes using PCR. The genetic typing of the strains was performed by RAPD-PCR. All strains fell into two clusters: A and B with dominant RAPD-groups A1 and B1, respectively, including 82% (107 of 130) of all studied strains. The susceptibility to the bacteriophage AP22 of the strains was determined. The phage was found to infect specifically and to constitute 69% of 130 strains and 82% (88 of 107) of the A. baumannii strains from the dominant RAPD groups. The ability of the bacteriophage AP22 to constitute a broad range of the clinically relevant A. baumannii strains makes it an attractive candidate for designing the phage cocktails intended to control the A. baumannii-associated nosocomial infections. Moreover, the phage can be used for the identification of A. baumannii in bacteriological analysis of clinical materials.
High-level production of a cold-active B-mannanase from Bacillus subtilis BS5 and its molecular cloning and expression.
Huang JL, Bao LX, Zou HY … +2 more , Che SG, Wang GX
Mol Gen Mikrobiol Virusol · 2012 · PMID 23248847
Mannanases can be useful in the food, feed, pulp and paper industries. In this research a Bacillus subtilis strain (named Bs5) which produced high-level beta-mannanase was isolated. Maximum level of beta-mannanase (1231....
Mannanases can be useful in the food, feed, pulp and paper industries. In this research a Bacillus subtilis strain (named Bs5) which produced high-level beta-mannanase was isolated. Maximum level of beta-mannanase (1231.41 U/ml) was reached when Bacillus subtilis Bs5 was grown on konjac powder as the carbon source for nine hours at 32 degrees C. The beta-mannanase was a typical cold-active enzyme and its optimal temperature of 35 degrees C was the lowest among those of the known mannanases from bacteria. In addition, the optimal pH was 5.0 and much wide pH range from 3.0-8.0 was also observed in the beta-mannanase. These properties make the beta-mannanase more attractive for biotechnological applications. The DNA sequence coding the beta-mannanase was cloned and the open reading frame consisted of 1089 bp encoding 362 amino acids. A phylogenetic tree of the beta-mannanase based on the similarity of amino acid sequences revealed that the beta-mannanase formed a cluster with the beta-mannanases of Bacillus subtilis, which was separated from the mannanases of fungi and other bacteria. The beta-mannanase gene could be expressed in Escherichia coli and the recombinant beta-mannanase was characterized by Western blot. This study provided a new source of carbohydrate hydrolysis enzyme with novel characteristics from Bacillus subtilis.
[Mobile ISCR elements: structure, functions, and role in the emergence, increasing and spreading of blocks of bacterial genes of multiple antibiotic resistance].
Il'ina TS
Mol Gen Mikrobiol Virusol · 2012 · PMID 23248846
The recently discovered method of horizontal distribution of bacterial genes with atypical ISCR sequences is reviewed using an example of drug resistance genes. The adjacent DNA segment mobilization is provided by the tr...
The recently discovered method of horizontal distribution of bacterial genes with atypical ISCR sequences is reviewed using an example of drug resistance genes. The adjacent DNA segment mobilization is provided by the transposition of such elements, including rolling circle replication, formation of autonomous nonreplicable circular structures, and homological recombination. The gene distribution capacity with the ISCR elements is more significant than the capacity of transposons and integrons, thereby providing formation of groups of mobile genes, including antibiotic-resistance genes of pathogenic bacteria. The structure and functions of the ISCR elements were discussed together with their similarity and dissimilarity with the group of IS91-similar elements and their role in the emergence of blocks of bacterial genes encoding of multiple antibiotic resistance and their contribution to evolution of bacterial and plasmid genes.
[New genotype of the sacbrood virus of the honeybee Apis mellifera].
Lomakina NF, Batuev IuM
Mol Gen Mikrobiol Virusol · 2012 · PMID 22984772
The sacbrood virus (SBV) leads to death of honeybee larvae. Until now, there were known three SBV genotypes: European, Asian, and South African (E. Grabensteiner et al., 2001; S. E. Choe et al., 2012). Serologic assay, s...
The sacbrood virus (SBV) leads to death of honeybee larvae. Until now, there were known three SBV genotypes: European, Asian, and South African (E. Grabensteiner et al., 2001; S. E. Choe et al., 2012). Serologic assay, sequencing and phylogenetic analysis of the SBV RNA-polymerase gene fragments resulted in discovery of two genotypes of SBV circulating among the honeybee Apis mellifera in the European region of Russian Federation (RF). One of them forms a new distinct genetic lineage of SBV (genotype 4), which has not been described before and has an intermediate position between the Asian and the South African genotypes on a phylogenetic tree. The viruses belonging to this group were isolated from honeybee in Kaluga region in 1986, and in 2006 from honeybee introduced earlier to Moscow from Uzbekistan. The sequence homology inside this group is 94.2%, whereas this one between different groups is not higher then 80.6-83.5%. Another group represents the European genotype of SBV circulating in Krasnodar Territory, Adyghe Republic, and Moscow region. Two SBV genotypes from Russian Federation can be differentiated using PCR and radial immunodiffusion test (RID). As for RID, both genotypes react with antiserum #6(1), but only the fourth genotype reacts with antiserum #58.
[Studying of molecular mechanisms of rubella virus attenuation evidence from Russian strain C-77].
Dmitriev GV, Borisova TK, Faĭzuloev EB … +3 more , Zabiiaka IuI, Desiatskova RG, Zverev VV
Mol Gen Mikrobiol Virusol · 2012 · PMID 22984771
Live attenuated rubella vaccine is used for vaccination. Temperature-sensitive (ts) phenotype was proved for almost all rubella vaccine strains, and the acquisition of the ts phenotype during cold adaptation was strongly...
Live attenuated rubella vaccine is used for vaccination. Temperature-sensitive (ts) phenotype was proved for almost all rubella vaccine strains, and the acquisition of the ts phenotype during cold adaptation was strongly correlated with the attenuation of the wild-type viruses. Nevertheless, the molecular mechanisms of the attenuation have been insufficiently understood for rubella virus. Study ofthese mechanisms, identifying genotypic markers of attenuation, which together with the sequence analyses could be used for genetic stability control of vaccine strains, is still of current interest. In this work, we determined nearly complete genome sequences of attenuated (ca) and the wildtype progenitor (wt) of the rubella virus strain C-77 isolated in Russia. Possible genetic determinants of attenuation were detected. Thus, 13 nucleotide differences leading to 6 amino acid substitutions were found. Four amino acid substitutions were found to be almost unique. Special consideration should be given to Tyr1042Cys substitution in the protease domain of C-77 strain, because it most probably plays the crucial role in acquisition of ts-phenotype.
[Specific motifs in the genomes of the family Chlamydiaceae].
Demkin VV, Kirillova NV
Mol Gen Mikrobiol Virusol · 2012 · PMID 22984770
Specific motifs in the genomes of the family Chlamydiaceae were discussed. The search for genetic markers ofbacteria identification and typing is an urgent problem. The progress in sequencing technology resulted in compi...
Specific motifs in the genomes of the family Chlamydiaceae were discussed. The search for genetic markers ofbacteria identification and typing is an urgent problem. The progress in sequencing technology resulted in compilation of the database of genomic nucleotide sequences of bacteria. This raised the problem of the search and selection of genetic targets for identification and typing in bacterial genes based on comparative analysis of complete genomic sequences. The goal of this work was to implement comparative genetic analysis of different species of the family Chlamydiaceae. This analysis was focused to detection of specific motifs capable of serving as genetic marker of this family. The consensus domains were detected using the Visual Basic for Application software for MS Excel. Complete coincidence of segments 25 nucleotide long was used as the test for consensus domain selection. One complete genomic sequence for each of 8 bacterial species was taken for the experiment. The experimental sample did not contain complete sequence of C. suis, because at the moment of this research this species was absence in the database GenBank. Comparative assay of the sequences of the C. trachomatis and other representatives of the family Chlamydiaceae revealed 41 common motifs for 8 Chlamydiaceae species tested in this work. The maximal number of consensus motifs was observed in genes of ribosomal RNA and t-RNA. In addition to genes of r-RNA and t-RNA consensus motifs were observed in 5 genes and 6 intergene segments. The gene CTL0299, CTLO800, dagA, and hctA consensus motifs detected in this work can be regarded as identification domains of the family Chlamydiaceae.
[The components of Francisella tularensis protective antigene complex].
Kuznetsova EM, Volokh OA, Shepelev IA … +1 more , Nikiforov AK
Mol Gen Mikrobiol Virusol · 2012 · PMID 22984769
The structural characteristics of Francisella tularensis protective antigene complex (PAC) were discussed. PAC is the water-soluble antigene of outer membranes F. Tularensis with sophisticated chemical nature. The molecu...
The structural characteristics of Francisella tularensis protective antigene complex (PAC) were discussed. PAC is the water-soluble antigene of outer membranes F. Tularensis with sophisticated chemical nature. The molecular weight of PAC is 280 kD. It was found that PAC is composed of some immunoreactivity protein subunits with molecular weight from 81 to 19 kD, and 14-17 kD lipoprotein subunit. The stress proteins-chaperones (GroES, GroEL), outer membrane proteins (FTL_0617, OmpH), receptor proteins (EF Tu, Rp1L), bacterial enzymes (KatG, GAPDH), and lipopolysaccharide were identified in the PAC structure. Their presence determines the PAC high immunobiological activity.
[The phylogeography of the Yersinia pestis vole strains isolated from the natural foci of caucasian region].
Platonov ME, Evseeva VV, Svetoch TE … +5 more , Efremenko DV, Kuznetsova IV, Dentovskaia SV, Kulichenko AN, Anisimov AP
Mol Gen Mikrobiol Virusol · 2012 · PMID 22984768
57 Y pestis bv. caucasica strains were assayed using molecular typing. The results of these assays indicated the presence within this biovar of the three separate clonal clusters and necessity of detachment of the Lenina...
57 Y pestis bv. caucasica strains were assayed using molecular typing. The results of these assays indicated the presence within this biovar of the three separate clonal clusters and necessity of detachment of the Leninakan mountain mesofocus (subfocus) from the structure of Transcaucasian-highland focus into self-supporting one, as well as inclusion of a part of the Pre-Araks low-mountain natural plague focus in the capacity of the subfocus along with Pre-Sevan mountain and Zanzegur-Karabakh mountain subfoci into the structure of Transcaucasian-highland focus. It was shown that the strains circulating in the East-Caucasian highland plague focus were the most ancient branch of bv. caucasica or even of the entire Y pestis phylogenetic tree.
Popular molecular markers in bacteria.
Liu W, Li L, Khan MA … +1 more , Zhu F
Mol Gen Mikrobiol Virusol · 2012 · PMID 22984767
Molecular markers are defined as the fragments of DNA sequence associated with a genome, which areused to identify a particular DNA sequence. Nowadays, with the explosive growth of genetic research and bacterial classifi...
Molecular markers are defined as the fragments of DNA sequence associated with a genome, which areused to identify a particular DNA sequence. Nowadays, with the explosive growth of genetic research and bacterial classification, molecular marker is an important tool to identify bacterial species. Taking account to its significant roles in clinic, medicine and food industry, in this review article, we summa rize the traditional research and new development about molecular markers (also called genetic markers) in bacteria, including genes of 16S rRNA, 23S rRNA, rpoB, gyrB, dnaK, dsrAB, amoA, amoB, mip, horA, hitAM, recA, ica, frc. oxc, 16S-23S rDNA ISR and IS256.
[The role of the type-three secretion system of the gram-negative bacteria in regulation of chronic infections].
Zigangirova NA, Nesterenko LN, Tiganova IG … +1 more , Kost EA
Mol Gen Mikrobiol Virusol · 2012 · PMID 22984766
The role of the type-three secretion system of the gram-negative bacteria in regulation of chronic infections is discussed. Recent research showed that most of severe chronic somatic diseases are derived from chronic inf...
The role of the type-three secretion system of the gram-negative bacteria in regulation of chronic infections is discussed. Recent research showed that most of severe chronic somatic diseases are derived from chronic infection induced in the first place by infectious agents. The role of the T3SS of different species in transition from an acute infection to persistence is reviewed. Clinical and bacteriological research showed that microorganisms are persistent in the form resistant to antibiotics. That is why one of the promising targets for the development of antibacterial new-generation treatment is T3SS that conducts transport of bacteria pathogenicity factors into eukaryotic cell. The presence of this structure is necessary for the development of an acute infectious process and chronization of an infection is essential for its functioning.
Construction of baculovirus expression vector of miRNAs and its expression in insect cells.
Huang Y, Zou Q, Shen XJ … +3 more , Yu XL, Wang ZB, Cheng XC
Mol Gen Mikrobiol Virusol · 2012 · PMID 22937569
MicroRNAs (miRNAs) are endogenous small non-protein coding RNAs that play important regulatory roles in animals and plants by binding to target transcripts for cleavage or translational repression. The miR-9a is very con...
MicroRNAs (miRNAs) are endogenous small non-protein coding RNAs that play important regulatory roles in animals and plants by binding to target transcripts for cleavage or translational repression. The miR-9a is very conservative in animals from flies to humans. Studies indicated that miR-9a is involved in the regulation of neurogenesis in animals. In our study, the baculovirus expression system was used to transcribe a recombinant vector containing miR-9a for further analysis the function ofmiR-9a. The sequence ofpre-miR-9a from silkworm DNA was first cloned into the donor pFastBac. The enhanced green fluorescent protein (EGFP) was used as reporter gene. The recombinant donor plasmid pFastBac-miR-9a was transformed into E.coli DH10Bac/AcNPV forming Bacmid-9a which was transfected into insect cells with cational lipofectin. The transcription of mature miR-9a was detected by Real-time PCR. The results show the recombinant Bacmid-9a was successfully constructed and effectively transcribed miR-9a in infected Sf21 insect cells.
[Use of multiple locus variable number tandem repeats analysis for the Brucella systematization].
Kulakov IuK, Kovalev DA, Misetova EN … +3 more , Golovneva SI, Liapustina LV, Zheludkov MM
Mol Gen Mikrobiol Virusol · 2012 · PMID 22937568
UNLABELLED: The methods of molecular-genetic differentiation to strain level acquire increasing significance in the current system of struggle with brucellosis. MLVA (multiple locus variable number tandem repeats analysi...
UNLABELLED: The methods of molecular-genetic differentiation to strain level acquire increasing significance in the current system of struggle with brucellosis. MLVA (multiple locus variable number tandem repeats analysis) was selected for molecular-genetic differentiation to strain level and simultaneous establishment of the genetic relationship of investigated Brucella strains. The goal of this work was MLVA typing of three pathogenic Brucella species strains with the analysis of stability of chosen loci, discrimination power and concordance to conventional phenotypic methods of the Brucella differentiation for use in systematization of brucellosis causing agents. MATERIALS AND METHODS: Twenty six Brucella strains representing reference (n = 15), vaccine (n = 2) and field strains of three pathogenic Brucella species were tested: B. melitensis (n = 3), B. abortus (n = 2), B. suis (n = 2), and isolates (n = 2) with unidentified taxonomic position using MLVA with 9 pairs primers on known variable loci of Brucella genome. The analysis of the stability of chosen loci, discrimination power on Hunter-Gaston discrimination index (HGDI) and consistency to phenotypic methods of identification was performed. RESULTS: MLVA was confirmed for the results of phenotypic methods of identification, stability of the chosen loci in majority reference, and vaccine strains with a high index of variability HGDI 0.9969 for all loci. A dendrogram was plotted on the basis of MLVA data on distributed Brucella strains in related clusters according to its taxonomic species and biovar positions and construction of 25 genotypes. B. melitensis strains formed cluster related to the reference strain of B. melitensis 63/9 biovar 2. Australian isolates of Brucella 83-4 and Brucella 83-6 isolated from rodents formed a cluster distant from other strains of Brucella. CONCLUSION: MLVA is a promising method for differentiation of Brucella strains with known and unresolved taxonomic status for their systematization and creation of MLVA genotype catalogue that will promote qualitative improvement of brucellosis surveillance system in Russia.
[Genotype diversity of the Bacillus anthracis strains isolated from the Caucasus region].
Eremenko EI, Riazanava AG, Tsygankova OI … +3 more , Tsygankova EA, Buravtseva NP, Kulichenko AN
Mol Gen Mikrobiol Virusol · 2012 · PMID 22937567
The study of the genotypes of Bacillus anthracis strains isolated from the Caucasus region was performed using MLVA. Among 149 strains, 32 distinctive MLVA-8 genotypes belonging to Ala, A3a, A4 and B1 molecular diversity...
The study of the genotypes of Bacillus anthracis strains isolated from the Caucasus region was performed using MLVA. Among 149 strains, 32 distinctive MLVA-8 genotypes belonging to Ala, A3a, A4 and B1 molecular diversity groups were identified. 9 genotypes were not described previously; 6 genotypes were not found in other geographic regions and could be considered as endemic for Caucasus. The majority of the identified genotypes are widespread not only in Caucasus, but also throughout Eurasia, Africa, and America. Molecular diversity of Caucasian isolates is comparable to the worldwide diversity. This represents historical relations of this region, proximity to ancient trade routes and intensity of the anthrax epizootic in Caucasus. The obtained results are of interest from the theoretical point of view, as well as for the application in epidemiological research of the anthrax outbreaks.
[Identification of the differences in the genes responsible for methionine biosynthesis in Bacillus anthracis strains and phylogeny-related species].
Mikshis NI, Zhivova IuN, Novikova LV … +3 more , Sharapova NA, Popov IuA, Kutyrev VV
Mol Gen Mikrobiol Virusol · 2012 · PMID 22937566
The comparative analysis of the gene sequences encoding the synthesis of enzymes responsible for the intermediary metabolism of methionine in Bacillus anthracis strains and in closely related bacterial species was carrie...
The comparative analysis of the gene sequences encoding the synthesis of enzymes responsible for the intermediary metabolism of methionine in Bacillus anthracis strains and in closely related bacterial species was carried out. Deletion of 42 nucleotides in the hom2 gene, which determines the homoserinedehydrogenase, is detected in all tested Bacillus anthracis strains. In the strains of other bacillar species hom2 gene mutation, which blocks up the tracts of methionine and threonine biosynthesis, was not identified. The single nucleotide polymorphism was determined in asd1, metX, and metH genes. It provides the identification of B. anthracis strains using sequencing technology.
[Molecular-genetic analysis of the epidemical strains of the Vibrio cholerae El Tor isolated from the Siberian and maritime regions of Russia].
Mironova LV, Balakhonov SV, urbanovich LIa … +4 more , Kozhevnikova AS, Polovinkina VS, Kulikalova ES, Afanas'ev MV
Mol Gen Mikrobiol Virusol · 2012 · PMID 22937565
The detection of the biotype-specificity, pathogenicity determinants, and sequencing of the ctxB gene and the ctxAB promoter was carried out for analysis of the Vibrio cholerae El Tor strains genome structure. The strain...
The detection of the biotype-specificity, pathogenicity determinants, and sequencing of the ctxB gene and the ctxAB promoter was carried out for analysis of the Vibrio cholerae El Tor strains genome structure. The strains (n = 90) were isolated during cholera epidemic outbreaks in Siberia and the Far East. All toxigenic Vibrio cholerae El Tor strains were divided into two groups: the first group included strains isolated during 1970s: they had the genotype ctxB3+rstREl+rstRCl-rstC+TLC+tbr4. All epidemic dangerous El Tor biotype strains isolated in 1990s belonged to the second group. The strains were characterized as atypical variants because of classical genotype (ctxB1) ctxB gene harboring. The second group fell into three genotypes according to the set of genetic markers (ctxB, rstR, rstC, TLC, tbr). It was suggested that the set of genetic determinants could be used as a marker for epidemiological analysis of spreading of atypical ET strains. The comparative analysis of genome structure enables to suggest possible ways of pathogen evolution.
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