Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia[JOURNAL]
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200 papers
[Study of the heterogeneity of 16s rRNA gene and groESL operone in the dna samples of Anaplasma phagocytophilum, Ehrlichia muris, and "Candidatus Neoehrlichia mikurensis" determined in the Ixodes persulcatus ticks in the area of Urals, Siberia, and far east of Russia].
Rar VA, Epikhina TI, Livanova NN … +5 more , Panov VV, Doroshenko EK, Pukhovskaia NM, Vysochina NP, Ivanov LI
Mol Gen Mikrobiol Virusol · 2011 · PMID 21786632
A total of 3552 Ixodes persulcatus from Sverdlovsk, Chelyabinsk, Novosibirsk, Irkutsk regions and Khabarovsk Territory were examined on the Ehrlichia and Anaplasma presence by nested PCR based on the 16S rRNA gene. Both...
A total of 3552 Ixodes persulcatus from Sverdlovsk, Chelyabinsk, Novosibirsk, Irkutsk regions and Khabarovsk Territory were examined on the Ehrlichia and Anaplasma presence by nested PCR based on the 16S rRNA gene. Both Anaplasma phagocytophilum and Ehrlichia muris DNA were found in I. persulcatus in all studied regions. A. Phagocytophilum was detected in 1.3-6.3% of ticks and E. muris - in 2.0-14.1% of ticks. Moreover, "Candidatus Neoehrlichia mikurensis" DNA was found in 8 ticks collected in Novosibirsk, Irkutsk Regions and Khabarovsk Territory. Partial nucleotide sequences of 16S rRNA gene and groESL operone (1240-1300 bp) were determined for 65 samples of A. Phagocytophilum, 17 samples of E. muris and 4 samples of "Candidatus Neoehrlichia mikurensis". Nucleotide sequences of 16S rRNA gene and groESL operone of E. muris and "Candidatus Neoehrlichia mikurensis" were shown to be highly conservative, and nucleotide sequences of groESL operone of both E. muris and "Candidatus Neoehrlichia mikurensis" differed from the sequences found previously in other species of Ixodid tick. On the basis of analysis of the 16S rRNA gene and groESL operone sequences it was concluded that all revealed samples A. Phagocytophilum could be divided into 2 groups. GroESL operone sequences of A. Phagocytophilum from the first group were identical to each other but significantly differed from the known groESL operone sequences (less than 98.2% of similarity), whereas their 16S rRNA gene sequences were identical to the sequence of widely distributed and pathogenic for human A. Phagocytophilum genetic variant (CAHU-HGEl, GenBank AF093788) or differed from it by a single nucleotide substitution. The nucleotide sequences of groESL operone of A. Phagocytophilum from the second group differed from each other by 1-4 nucleotides and were closely related (99.2-99.4% of similarity) to the sequences of groESL operone ofA. phagocytophilum isolates found in Europe in Ixodes ricinus and roe deer. The nucleotide sequences of the 16S rRNA gene of A. Phagocytophilum from the second group were most similar to the sequence of the rare A. Phagocytophilum genetic variant previously found only in China (GenBank DQ342324).
[Genetic features of Borrelia miyamotoi transmitted by Ixodes persulcatus].
Fomenko NV, Borgoiakov VIu, Panov VV
Mol Gen Mikrobiol Virusol · 2011 · PMID 21786631
The definition and molecular typing of Borrelia miyamotoi transmitted by the Ixodes persuccatus ticks was based on the partial sequencing of the 16S rRNA, p66, and glpQ genes. All the B. miyamotoi analyzed sequences of t...
The definition and molecular typing of Borrelia miyamotoi transmitted by the Ixodes persuccatus ticks was based on the partial sequencing of the 16S rRNA, p66, and glpQ genes. All the B. miyamotoi analyzed sequences of the 16S rRNA, glpQ, and p66 gene fragments from I. persulcatus were identical and had 99.9-100% similarity to corresponding genes of the B. miyamotoi strain FR64b isolated in Japan. The analyzed amino acid sequences revealed that the 66 protein B. miyamotoi in the site corresponding to the surface-exposed domain contained considerable difference from the Borrelia hermsii, the typical member tick-born relapsing fever, as from Borrelia lonestari transmitted by the Ixodes ticks.
[Genetic characterization of the Brucella melitensis isolates from Mongolia, Russia, and Azerbaijan].
Kulakov IuK, Erdenbaator J, Zheludkov MM … +1 more , Korenberg EI
Mol Gen Mikrobiol Virusol · 2011 · PMID 21786630
UNLABELLED: The goal of this work was to provide comparative genetic characterization of the human and animal Brucella melitensis isolates from Mongolia, Russia and Azerbaijan using current molecular-genetic typing metho...
UNLABELLED: The goal of this work was to provide comparative genetic characterization of the human and animal Brucella melitensis isolates from Mongolia, Russia and Azerbaijan using current molecular-genetic typing methods. MATERIALS AND METHODS: Twenty eight Mongolian (n = 18), Russian (n = 6), and Azerbaijan (n = 4) human and animal Brucella melitensis isolates were studied using 2 molecular typing methods based on PCR for differential species and biovar specific ORF (open reading frames) molecular targets and MLVA (multiple locus variable number tandem repeats analysis) using primers to 12 known loci. RESULTS: The PCR was used for differential molecular targets (all B. melitensis isolates) were characterized as the B. melitensis biovar 2. The MLVA revealed 7 identical and 5 variable MLVA loci. All the isolates were classified into 25 genotypes using the dendrogram on the data of 12 loci and the cluster related to reference strain B. melitensis 63/9 biovar 2. The B. melitensis isolates having related MLVA genotypes were connected to Mongolian, Russian and Azerbaijan regions. The circulation for two B. melitensis isolates to not typical hosts as camel and yak was demonstrated using molecular typing methods. CONCLUSION: The genetic characterization of twenty eight B. melitensis isolates from different geographical regions in Mongolia, Russia, and Azerbaijan recognized genetic relationships. On the other hand, the MLVA has high discrimination power with a resulting Hunter and Gaston discriminatory index (HGDI) of 0.9841 revealing genetic diversity for the isolates forming of 25 MLVA genotypes. To improve the system of the brucellosis surveillance in Russia MLVA typing of B. melitensis isolates are necessary to investigate from the Siberian (Republics Tuva, Buryatia, and Irkutsk region) and South (Republics Dagestan, Kalmykia, and Stavropol region) Districts having frontier areas with Mongolia and Azerbaijan.
[Analysis of the polymorphic variants of the PDE4D gene in patients with acute stroke in the Moldavian population].
Bondarenko EA, Mokan EI, Barbakar NI … +7 more , Tupitsyna TV, Slominskiĭ PA, Shetova IM, Shamalov NA, Botsina AIu, Skvortsova VI, Limborskaia SA
Mol Gen Mikrobiol Virusol · 2011 · PMID 21786629
The risk of the ischemic stroke is mediated by both environmental and genetic factors. Recent studies of DeCode group identified the risk of polymorphisms for ischemic stroke in the phosphodiesterase 4D gene (PDE4D). The...
The risk of the ischemic stroke is mediated by both environmental and genetic factors. Recent studies of DeCode group identified the risk of polymorphisms for ischemic stroke in the phosphodiesterase 4D gene (PDE4D). The goal of this study was to explore the role of two variants of the gene encoding PDE4D [SNP41 (rs152312) and SNP87 (rs2910829)] in the Moldavian patients with ischemic stroke and in control. No significant association with ischemic stroke was observed with SNP41 and 87.
[Construction of the genetically attenuated bacteria Bordetella pertussis devoid of dermonecrotic toxin activity and producing modified nontoxic pertussis toxin form].
Siniashina LN, Siniashina LS, Semin EG … +2 more , Amelina IP, Karataev GI
Mol Gen Mikrobiol Virusol · 2010 · PMID 20886687
The recombinant modified (attenuated) bacteria A. pertussis were constructed. These bacteria contained knockout mutation of the dnt gene and produced nontoxic pertussis toxin derivative. The immunological properties of t...
The recombinant modified (attenuated) bacteria A. pertussis were constructed. These bacteria contained knockout mutation of the dnt gene and produced nontoxic pertussis toxin derivative. The immunological properties of the mutant bacteria B. pertussis strain KS were studied. The recombinant bacteria B. pertussis strain KS were found to be devoid of dermonecrotic toxin activity, conserved the structure of the mutant dnt gene in condition of cultivation on selective growth media, and long-term survival in laboratory animal organism. Intranasal immunization of mice with living bacteria B. pertussis, attenuated strain KS provided protection of animals from virulent strains of the pertussis. The efficiency of the protection was comparable with protection efficiency provided by standard corpuscular pertussis vaccine OSO-3.
[Detection of Babesia spp. DNA in small mammals and ixodic ticks on the territory of north Ural, west Siberia and far east of Russia].
Rar VA, Epikhina TI, Livanova NN … +4 more , Panov VV, Pukhovskaia NM, Vysochina NP, Ivanov LI
Mol Gen Mikrobiol Virusol · 2010 · PMID 20886686
Totally, 932 small mammals and 458 questing adult Ixodes persulcatus from Sverdlovsk and Novosibirsk regions and Khabarovsk Territory, as well as 128 Haemaphysalis japonica, 34 H. concinna and 29 Dermacentor silvarum fro...
Totally, 932 small mammals and 458 questing adult Ixodes persulcatus from Sverdlovsk and Novosibirsk regions and Khabarovsk Territory, as well as 128 Haemaphysalis japonica, 34 H. concinna and 29 Dermacentor silvarum from Khabarovsk Territory were examined for the presence of Babesia by nested PCR based on the 18S rRNA gene. Babesia microti DNA was found in samples of small mammals from all the studied regions--in 36.2% of samples from Sverdlovsk region, 5.3% of samples from Novosibirsk region, and 6.7% of samples from Khabarovsk Territory. The determined B. microti 18S rRNA gene sequences from Novosibirsk region (6 sequences) and from Khabarovsk Territory (10 sequences) were identical to each other and to the sequences of pathogenic for human B. microti US-type, while the determined B. microti 18S rRNA gene sequences from Sverdlovsk region (12 sequences) were identical to those of B. microti strain Munich. B. microti were found most frequently in samples of Myodes spp., they were found also in Microtus spp., Apodemus spp., Sorer spp., and Sicista betulinav. It was shown that one of 347 analyzed I. persulcatus from Novosibirsk region and one of 77 I. persulcatus from Khabarovsk Territory contained B. microti US-type DNA. One I. persulcatus from Novosibirsk region contained B. divergens DNA. In this work B. divergens was for the first time determined in I. persulcatus and B. microti in I. persulcatus in Asian part of Russia. Three different genetic variants of Babesia sensu stricto were found in three H. japonica from Khabarovsk Territory. The first genetic variant was closely related to Babesia sp. revealed in a feral raccoon in Japan (99.9% similarity on the basis of 18S rRNA gene sequences). Two others Babesia genetic variants were most similar to the ovine pathogen Babesia crassa (97.1-97.6% similarity on the basis of 18S rRNA gene sequences).
[The role of polymorphic variants of gene of inducible NO-synthase NOS2 in brain infarction in patients with acute ischemic stroke].
Tupitsyna TV, Bondarenko EA, Botsina AIu … +4 more , Shetova IM, Limborskaia SA, Skvortsova VI, Slominskiĭ PA
Mol Gen Mikrobiol Virusol · 2010 · PMID 20886685
Cerebrovascular diseases including stroke are an important problem of public health. Stroke development depends on external factors and individual genetic specificity of patient. Excessive NO production by inducible NO-s...
Cerebrovascular diseases including stroke are an important problem of public health. Stroke development depends on external factors and individual genetic specificity of patient. Excessive NO production by inducible NO-synthase (iNOS) damages brain tissue at various stages of the disease. The goal of this work was to study the role of 4 polymorphic variants of gene of inducible NO-synthase iNOS (-2447C/G, -1659C/T, -0,7(OTTA)n I/D, S608L (150C/T)) in brain infarction in patients with acute ischemic stroke. A statistically significant correlation between S608L (150C/T) polymorphism and infarction dynamics was observed during days 1-3 and 7-21 after infarction. These parameters correlated with neurological status estimated using the Orgogozo scale during days 1-7 of the disease development. It was demonstrated that genotype N150N was associated with ischemic focus propagation regardless of its volume and neurological status by Orgogozo scale in patients with acute stroke. It was also observed that genotype N150N had effect on ischemic damage during days 1-3 in case of low initial volume.
[Tabular excel editor for analysis of aligned nucleotide sequences].
Demkin VV
Mol Gen Mikrobiol Virusol · 2010 · PMID 20882774
Excel platform was used for transition of results of multiple aligned nucleotide sequences obtained using the BLAST network service to the form appropriate for visual analysis and editing. Two macros operators for MS Exc...
Excel platform was used for transition of results of multiple aligned nucleotide sequences obtained using the BLAST network service to the form appropriate for visual analysis and editing. Two macros operators for MS Excel 2007 were constructed. The array of aligned sequences transformed into Excel table and processed using macros operators is more appropriate for analysis than initial html data.
[Development of the PCR-test system for detection primate betaherpesvirinae].
Agumava AA, Chikobava MG, Lapin BA
Mol Gen Mikrobiol Virusol · 2010 · PMID 20882773
A PCR-test system for detection of primate Betaherpesvirinae viruses was developed. Using alignment of complete genome of human, chimpanzee, and macaque rhesus cytomegalovirus conserve regions of viral genes were found....
A PCR-test system for detection of primate Betaherpesvirinae viruses was developed. Using alignment of complete genome of human, chimpanzee, and macaque rhesus cytomegalovirus conserve regions of viral genes were found. The oligonucleotide primers for consensus conserve regions of CMV UL56 gene were developed. The conditions of PCR were optimized and primer specificity for cytomegaloviruses of different primate species was confirmed.
[A mass-spectrometric analysis of genetic markers of S. pneumoniae resistance to beta-lactam antibiotics].
Savinova TA, Il'ina EN, Sidorenko SV
Mol Gen Mikrobiol Virusol · 2010 · PMID 20882772
Beta-lactam antibiotics remain the drugs of choice for treatment of S. pneumoniae infections in spite of growing level of resistance. The formation of S. pneumoniae resistance to these drugs is mediated by modifications...
Beta-lactam antibiotics remain the drugs of choice for treatment of S. pneumoniae infections in spite of growing level of resistance. The formation of S. pneumoniae resistance to these drugs is mediated by modifications of the penicillin-binding proteins (PBPs), the targets of the antibiotic action. A new approach to detection of mutations in PBP1A, 2B and 2X genes based on minisequencing reaction followed by MALDI-ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry was developed in this study. The evaluation of these mutations prevalence in clinical S. pneumoniae isolates (n = 194) with different susceptibility level to beta-lactam antibiotics was performed. Twenty-four different combinations of mutations in PBPs (genotypes) were detected. All isolates susceptible to penicillin (n = 49, MIC > or = 0.06 > or = gamma/ml) carried no mutations in all analyzed loci. For 145 S. pneumoniae isolates with reduced susceptibility to penicillin (MIC > 0.06 > or = gamma/ml) the mutations in PBPs were detected in 133 (91.7 %) cases that testify to high diagnostic sensitivity of such approach. The isolates with MIC > or = 4 > or = gamma/ml (n = 20) carried multiple mutations in all analyzed genes that confirms cumulative effects of penicillin resistance formation. However, it was not possible to associate observed mutations in PBPs genes with decrease of susceptibility to cefotaxime that allows suggesting the entire difference in molecular mechanisms of formation of resistance to penicillins and cephalosporins. The offered method of S. pneumoniae genotyping is suitable for susceptibility testing to penicillin of individual isolates and for molecular monitoring of the resistance determinants in population.
[Development of pulmonary chlamydia infection in inbred mice lines differentiated by genetically determinated sensitivity to tuberculosis infection].
Nesterenko LN, Aliapkina IuS, Pashko IuP … +6 more , Kondrat'eva EV, Kapina MA, Balunets DV, Zagangirova NA, Romanova IuM, Apt AS
Mol Gen Mikrobiol Virusol · 2010 · PMID 20882771
Mice of I/St strain develop severe lung inflammation and die shortly following infection with virulent mycobacteria. The susceptibility does not depend on the Nramp1 gene, as I/St mice carry its resistant allele, but is...
Mice of I/St strain develop severe lung inflammation and die shortly following infection with virulent mycobacteria. The susceptibility does not depend on the Nramp1 gene, as I/St mice carry its resistant allele, but is controlled by little interacting QTL mapped to chromosomes 3, 9, 17. To find out whether the tuberculosis-susceptible I/St mice are susceptible to other intracellular bacteria taxonomically distant pathogen of Chlamydia pneumoniae was studied. Comparison of I/St and TB-resistant A/Sn mice (both Nramp1r) demonstrated that the former were more susceptible to chlamydia, displaying a significantly shortened survival time following challenge (I/St, 9.2 +/- 1.2 days; A/Sn, 22.0 +/- 0 days (p < 0.001)). To estimate the degree of chlamydial multiplication in the lungs, we suggested a quantitative real-time polymerase chain reaction (PCR)-based method which allows enumeration of the parasite's genome equivalents in infected tissue from 1 to 16 days after challenge. The interstrain difference of chlamydia burden in lungs was observed only after 24 hours after infection. Multiplication of chlamydia in the lungs was controlled efficiently after day 4 of infection. The numbers of genome equivalents dropped slightly by day 8 both in I/St and A/Sn mice. Lung pathology develops more rapidly in I/St compared to A/Sn mice following infection with chlamydia despite their similar ability to control bacterial multiplication. Lung tissue of susceptible I/St mice was markedly infiltrated with macrophages (p < 0.01), which differed significantly from the lungs of resistant A/Sn mice. In agreement with higher macrophage content in the lungs, significantly more macrophage-derived proinflammatory cytokines TNF-? and IL-6 were detected in lung tissue homogenates obtained from I/St mice (p < 0.05). Because the prominent difference in survival time did not correlate with permanent difference in bacterial multiplication, we suggested that both infections trigger fatal pathological processes whose dynamics depends strongly upon the host genetics.
[Genetic variants of Borrelia garinii, a widely spread Eurasian pathogen of ixodic tick borreliosis].
Nefedova VV, Korenberg EI, Gorelova NB
Mol Gen Mikrobiol Virusol · 2010 · PMID 20882770
As a result of PCR-RFLP analysis and the degree of similarity between the nucleotide sequences analysis of the rrfA-rrlB intergenic spacer DNA of 227 primary isolates of Borrelia garinii and 71 isolates/ amplicons from G...
As a result of PCR-RFLP analysis and the degree of similarity between the nucleotide sequences analysis of the rrfA-rrlB intergenic spacer DNA of 227 primary isolates of Borrelia garinii and 71 isolates/ amplicons from GenBank database in different regions of Eurasia revealed significant intraspecific heterogeneity among those of Borrelia. It was shown that genospecies B. garinii had within the two genetic subgroups (20047 and NT29) 16 genetic variants, whose geography was likely to be different.
[An in vivo study of the biologic activity of the recombinant pseudoadenovirus nanostructure containing lactoferrin gene].
Iakubovskaia RI, Bezborodova OA, Nemtsova ER … +6 more , Tutykhina IL, Shmarov MM, Logunov DIu, Naroditskii BS, Chissov VI, Gintsburg AL
Mol Gen Mikrobiol Virusol · 2010 · PMID 20545044
The Ad5-Lf recombinant pseudoadenovirus nanostructure (RPAN) based on adenovirus of the 5th serotype and containing lactoferrin (Lf) gene was constructed. The goal of this work was to develop a system for efficient produ...
The Ad5-Lf recombinant pseudoadenovirus nanostructure (RPAN) based on adenovirus of the 5th serotype and containing lactoferrin (Lf) gene was constructed. The goal of this work was to develop a system for efficient production of human lactoferrin (Lf) in human body. It was shown using the model of cisplatin (DDP)-induced toxicosis that human Ad5-based RPAN with human Lf gene expressing cassette in its genome provides high rate of expression of Lf gene in animal body. In vivo recombinant human Lf demonstrates detoxification effect against acute DDP-induced toxic reactions similar to that of the native Lf. RPAN does not stimulate growth of primary and metastatic nodes of experimental tumors. Moreover, it inhibits the growth of Lewis lung carcinoma (LLC), Ehrlich carcinoma (ELD), and S37 sarcoma in early periods after tumor transplantation. The obtained experimental data are indicative of the good prospects of further biologic and medical study of RPAN and development of RPAN-based genetic engineering medicine of the new generation.
[Repeats in bacterial genomes: evolutionary considerations].
Smirnov GB
Mol Gen Mikrobiol Virusol · 2010 · PMID 20545043
Contemporary data on repeated nucleotide sequences (repeats) in bacterial genomes are discussed. Different classifications and distribution of the repeats in the genomes of bacteria belonging to different species are rev...
Contemporary data on repeated nucleotide sequences (repeats) in bacterial genomes are discussed. Different classifications and distribution of the repeats in the genomes of bacteria belonging to different species are reviewed. Comparative data on the density of repeats in the genomes of pathogenic and non-pathogenic bacteria, as well as in the genes encoding different functions of microbial cell (genes of stress response, genes controlling DNA metabolism and repair, etc.), are discussed. The role of the repeats in the uptake and loss of genetic material by bacterial genomes is discussed. It is suggested that repeats are responsible not only for the functioning of bacterial genome at the given moment of time, but also for the structures of the implicit genomes, which may appear in the future due to evolutionary significant genomic rearrangements.
[Pdcd4 tumor suppressor: properties, functions, and their application to oncology].
Vikhreva PN, Shepelev MV, Korobko EV … +1 more , Korobko IV
Mol Gen Mikrobiol Virusol · 2010 · PMID 20545042
Inactivation of tumor suppressors and activation of protooncogenes are critical events in malignant cell transformation and tumor progression. Pdcd4 encodes a protein with tumor suppressor functions, which accounts for a...
Inactivation of tumor suppressors and activation of protooncogenes are critical events in malignant cell transformation and tumor progression. Pdcd4 encodes a protein with tumor suppressor functions, which accounts for an increased interest to Pdcd4 as a potential diagnostic and prognostic marker, as well as a target for antineoplastic therapy. This review summarizes well-known properties and functions of Pdcd4 tumor suppressor and mechanisms of its regulation in tumor cells. It is also focused to the role of Pdcd4 in cellular transformation and tumor progression, as well as on its potential practical application in oncology.
[A study of the single nucleotide polymorphism in seven genes (GHR, IGFBP3, IGFR1, IRS1, FMN1, ANXA2, TaGLN) in ethnic Russians and in patients with prostate cancer].
Lisitskaia KV, Krakhmaleva IN, Shishkin SS
Mol Gen Mikrobiol Virusol · 2010 · PMID 20540360
Using the RT-PCR method for allele discrimination, we examined nine known SNPs in seven genes (GHR, IGFBP3, IGFR1, IRS1, FMN1, ANXA2, TaGLN) in ethnic Russians and in patients with prostate cancer (PC). For Russian popul...
Using the RT-PCR method for allele discrimination, we examined nine known SNPs in seven genes (GHR, IGFBP3, IGFR1, IRS1, FMN1, ANXA2, TaGLN) in ethnic Russians and in patients with prostate cancer (PC). For Russian population data on genotype distribution in studied SNPs was obtained. It was revealed that six of nine analyzed sites in examined locus were polymorphic. Distributions of alleles and genotypes frequency of polymorphic site 1388 T/C (Leu463Pro) in gene FMN1 (rs2306277) were distinguished between patients and control groups (delta = 0.019; chi2 = 7.884). In particular, correlation of OO genotype with increased risk of PC was observed (OR = 2.1591 95% CI 1.2055-3.8726). Moreover, the analysis of the polymorphic site 2911G/A (Glu917Arg) in gene IRS1 (rs1801278) revealed the accumulation of allele A in cancer group in comparison with control group (chi2 = 4.038; p = 0.044). Thus, the obtained data indicate the possibility of participation of polymorphism in genes FMN1 and IRS1 in formation of predisposition to PC.
[Activation of the bioluminescence of the sensor Escherichia coli strains used for detecting N-acyl-homoserine lactones in the presence of nitrofurans and NO generators].
Zaĭtseva IuV, Granik VG, Belik AS … +2 more , Koksharova OA, Khmel' IA
Mol Gen Mikrobiol Virusol · 2010 · PMID 20540359
Nitrofurans (nitrofurazone, nitrofurantoin, furazidin, nifuroxazide), and nitric oxide generators (sodium nitroprusside and isosorbide mononitrate) in subinhibitory concentrations were shown to significantly increase the...
Nitrofurans (nitrofurazone, nitrofurantoin, furazidin, nifuroxazide), and nitric oxide generators (sodium nitroprusside and isosorbide mononitrate) in subinhibitory concentrations were shown to significantly increase the bioluminescence of the sensor Escherichia coli strains used for detecting N-acyl-homoserine lactones, signaling molecules of Quorum Sensing (QS) regulatory systems. The highest activation of bioluminescence (up to 250-400 fold) was observed in the presence of nitrofurazone on E. coli DH5alpha biosensors containing lux-reporter plasmids pSB401 or pSB536. However, this activation was not specifically associated with the functioning of QS systems. We suggest that the effect observed results from a direct action of nitrofurans and NO donors on the process of bioluminescence. The data indicate the necessity of using the biosensors that make it possible to detect specific effects of substances tested on QS regulation.
[Molecular characteristic of methicillin-resistant Staphylococcus aureus strains isolated in Moscow clinics].
Afanas'ev MV, Karakashev SV, Il'ina EN … +3 more , Salem Al-Salami AM, Sidorenko SV, Govorun VM
Mol Gen Mikrobiol Virusol · 2010 · PMID 20540358
UNLABELLED: OBJECTIVES; Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen of nosocomial infection. The goal of this work was to evaluate the clonality of hospital-acquired MRSA (HA-MRSA) circulating...
UNLABELLED: OBJECTIVES; Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen of nosocomial infection. The goal of this work was to evaluate the clonality of hospital-acquired MRSA (HA-MRSA) circulating in Russian Federation and to compare different multiplex PCR techniques with SNP-based approach for MRSA typing. METHODS: Epidemiologically unrelated MRSA isolates (n = 62) from Moscow hospitals were selected for typing. Genomic DNA from clinical isolates was purified using the DNA express kit (Lytech Ltd, Russia). Staphylococcus chromosomal cassette mec (SCCmec) typing was performed by PCR using the previously described methods. Seven loci from five housekeeping genes (arcC162, arcC210, aroE132, gmk123, tpi241, tpi243 and yqiL333) were used for SNP-typing. Detection of particular nucleotides in selected loci was carried out in the thermocyclic primer extension reaction, followed by mass spectrometry of the products. Standard MLST procedure was performed as reference method. RESULTS: The majority of the MRSA isolates (93.6%) belong to world-wide disseminated clonal complex (CC) 8. Three isolates (4.8%) belong to CC 1. All ST 239 isolates were found to carry SCCmec type III; ST 8 isolates, SCCmec type IV. CONCLUSION: Among Russian MRSA CC 8 isolates carrying SCCmec IV type are predominant. SNP-typing is powerful toll for studies of molecular epidemiology of MRSA.
[Molecular-genetic analysis of the Batai virus strains isolated from mosquitoes in Volgograd Region of the Russian Federation, West Ukraine, and Czech Republic].
Terekhin SA, Grebennikova TV, Khutoretskaia NV … +1 more , Butenko AM
Mol Gen Mikrobiol Virusol · 2010 · PMID 20364478
Partial nucleotide sequence of the M-segment from five Batai virus strains was determined. These strains were isolated in Volgograd Region, West Ukraine, and Czech Republic. Our data based on the partial sequence of the...
Partial nucleotide sequence of the M-segment from five Batai virus strains was determined. These strains were isolated in Volgograd Region, West Ukraine, and Czech Republic. Our data based on the partial sequence of the M-segment of Batai virus strains demonstrated that the strains isolated in Russian Federation, Ukraine, and Czech Republic grouped together into an European genetic group that was distinct from Asian and African strains of Batai virus.
[Genotyping some Borrelia burgdorferi sensu lato isolates from Ixodes ricinus ticks in Russia and Ukraine].
Nefedova VV, Korenberg EI, Gorelova NB
Mol Gen Mikrobiol Virusol · 2010 · PMID 20364477
The 4 Borrelia burgdorferi sensu lato isolates obtained from 1. Ricinus ticks collected in the natural foci in Russia and Ukraine, having an unusual RFLP Msel-pattern, were studied using sequencing rrfA-rrlB spacer and r...
The 4 Borrelia burgdorferi sensu lato isolates obtained from 1. Ricinus ticks collected in the natural foci in Russia and Ukraine, having an unusual RFLP Msel-pattern, were studied using sequencing rrfA-rrlB spacer and rrs gene. The Ir-5215 isolate from the tick collected in southern Ukraine represented recently described genospecies B. spielmanii pathogenic for humans. The three atypical isolates Ir-3519, Ir-4721, and Ir-4812 had 100% identity with the sequence of the atypical European B. burgdorferi sensu stricto strains. They constituted a subgroup of the B. burgdorferi sensu stricto on the grounds of Multilocus sequence analysis (MLSA). These data can be indicative of the genetic heterogeneity of the current group B. burgdorferi sensu stricto.
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