J Med Food [JOURNAL]
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200 papers
Extract Improves Insulin Sensitivity and Glucose Homeostasis by Activating Insulin Signaling and Glucagon-like Peptide-1 Modulation.
Jung J, Park SH, Cho W … +4 more , Lee M, Kim J, Gwon Y, Kim OK
J Med Food · 2025 Oct · PMID 40523228 · Publisher ↗
This study evaluated the effects of on glucose metabolism, insulin signaling, and key metabolic markers in type 2 diabetes (T2D). We evaluated how influences the activity of major enzymes responsible for carbohydrate b...
This study evaluated the effects of on glucose metabolism, insulin signaling, and key metabolic markers in type 2 diabetes (T2D). We evaluated how influences the activity of major enzymes responsible for carbohydrate breakdown. studies on C2C12 cells also examined glucose uptake and insulin signaling pathway activation. Otsuka Long-Evans Tokushima Fatty (OLETF) rats were treated with (25 and 50 mg/kg body weight [bw]) or metformin (126 mg/kg bw) for 16 weeks suppressed the activities of α-glucosidase and α-amylase while promoting insulin signaling pathways and glucose uptake in C2C12 myotubes. In OLETF rats, administration of markedly enhanced glucose tolerance, lowered fasting blood glucose and HbA1c levels, and significantly decreased the homeostatic model assessment for insulin resistance (HOMA-IR) index, reflecting improved insulin sensitivity. Western blot analysis revealed increased insulin receptor substrate-1, PI3K, Akt, and activated protein kinase (AMPK) phosphorylation, along with upregulated GLUT4 expression in skeletal muscle. Serum glucagon-like peptide-1 (GLP-1) and adiponectin levels were significantly elevated in the -treated groups, helping improve insulin action and systemic metabolic regulation. As a result, exerts beneficial effects on insulin sensitivity and glucose homeostasis by affecting key mechanisms such as insulin signaling, incretin dynamics, and adipokine modulation, reinforcing its value as a n'atural agent for treating T2D and associated metabolic issues.
Ursolic Acid Attenuates Inflammatory and Severity of Osteonecrosis in Rats Treated with Zoledronic Acid.
Coelho LMC, Carvalho LM, Mota Lemos JV … +7 more , Pessoa CDÓ, Bezerra MJB, Lima LVR, Lima Verde MEQ, Barros Silva PG, Sousa FB, Dantas TS
J Med Food · 2025 Sep · PMID 40522629 · Publisher ↗
Bisphosphonate (BP)-related osteonecrosis of the jaw (BRONJ) alters osteoclast function. Ursolic acid (UA) can inhibit the expression of interleukin-17 (IL-17). Evaluate the influence of UA treatment on the severity of B...
Bisphosphonate (BP)-related osteonecrosis of the jaw (BRONJ) alters osteoclast function. Ursolic acid (UA) can inhibit the expression of interleukin-17 (IL-17). Evaluate the influence of UA treatment on the severity of BRONJ in zoledronic acid (ZA)-treated rats' jaws. Fifty male Wistar rats were used, divided into a negative control group (0.1 mL/kg sterile saline), a positive control group (ZA, 0.20 mg/kg), and three test groups treated with ZA and UA 10, 20 or 40 mg/kg by gavage every three days from the beginning of the protocol until euthanasia. After three consecutive weekly administrations of ZA intravenous (i.v.), exodontia of the 1st left lower molar was performed, administration of an additional dose of ZA, and euthanasia after 28 days from exodontia. Hemimandibles were removed for radiographical, histological, and immunohistochemical analysis and gum samples for Western blotting. The femur was removed for the three-point bending test. Analysis of variance (ANOVA)/Bonferroni was used. Radiographically, UA reduced the area suggestive of OM (7.2 ± 0.6 vs. 5.2 ± 0.4, = .015) and the highest dose of UA reversed the number of nonviable osteocytes (80.3 ± 4.9 vs. 55.4 ± 4.6, = .007), suggesting bone healing through IL-17 inhibition. UA reduced the number of polymorphonuclear cells (208 ± 17 vs. 30 ± 9, < .001), mononuclear cells (207 ± 34 vs. 74 ± 20, < 0.001) and apoptotic osteoclasts (87 ± 4 vs. 61 ± 3, < .001), observing that these parameters are higher in groups treated only with ZA. The two highest doses of UA reduced the immunoexpression of IL-17 (429 ± 45 vs. 300 ± 42, = .014) and increased the percentage of circulating lymphocytes (69 ± 2 vs. 82 ± 2, < .001). AZ increased the expression of RORyT and the highest dose of UA (1.887 ± 0.114 vs. 0.869 ± 0.050, < .001),) reduced this expression, suggesting that UA may be a specific antagonist of RORyT, which has the capacity to inhibit the expression of this protein. UA promise in reducing the severity of ZA-induced ONJ.
Oral Collagen Oligopeptides as a Modulator of Skin Health: A Comprehensive Evaluation of Clinical and Molecular Effects.
Zague V, Pinheiro ALTA, Pinto JR … +2 more , Facchini G, Eberlin S
J Med Food · 2025 Sep · PMID 40518844 · Publisher ↗
Collagen peptides (CPs) have been recognized for their potential to enhance skin health, particularly by improving hydration, firmness, and elasticity. However, the molecular mechanisms driving these benefits remain not...
Collagen peptides (CPs) have been recognized for their potential to enhance skin health, particularly by improving hydration, firmness, and elasticity. However, the molecular mechanisms driving these benefits remain not fully understood, especially regarding their influence on essential extracellular matrix (ECM) components and enzymes that regulate collagen turnover. This study investigated both the clinical efficacy and the underlying preclinical molecular effects of oral collagen oligopeptides (Col-OP) supplementation, aiming to clarify how these peptides contribute to skin improvements. In clinical trials, a double-blind, randomized, placebo-controlled study with 85 women aged 45-60 years examined the effects of daily 2.5 g Col-OP supplementation over 84 days, measuring skin hydration (Corneometer®), firmness, and elasticity (Cutometer®). In addition to clinical studies, preclinical experiments were conducted on human dermal fibroblast cultures to elucidate the molecular effects of Col-OP. Fibroblasts were treated with noncytotoxic concentrations of Col-OP (10.0, 3.16, and 1.0 mg/mL) for 96 h, assessing the synthesis of type I collagen, matrix metalloproteinase-1 (MMP-1), tissue inhibitor of matrix metalloproteinases (TIMP-1), decorin, versican, biglycan, and hyaluronic acid (HA) through gene expression and enzyme-linked immunosorbent assays. Results showed that Col-OP treatment significantly enhanced gene expression of type I procollagen, decorin, and biglycan while decreasing versican levels ( < .001). It also promoted type I collagen synthesis and TIMP-1 levels, modulating MMP-1 and HA production. Clinically, Col-OP significantly enhanced skin firmness and elasticity compared with placebo ( < .05), while improvements in skin hydration did not achieve statistical significance. Based on the current data, it can be concluded that oral supplementation with Col-OP effectively enhances skin health by promoting key ECM components and modulating collagen turnover, offering a promising approach for improving skin health.
Genotoxicity Evaluation of Biokesum® () Standardized Extract Using Bacterial Reverse Mutation, Micronucleus, and Chromosomal Aberration Studies.
Ator J, Kelly M, Shivaram R … +3 more , Krisnamurthy RC, Soman YP, Whittaker MH
J Med Food · 2025 Sep · PMID 40518833 · Publisher ↗
is a shrub native to Southeast Asia that is commonly known as "kesum." Its leaf extract is used medicinally and in dietary supplements. To add to the overall toxicological dataset, Biokesum® () standardized extract was...
is a shrub native to Southeast Asia that is commonly known as "kesum." Its leaf extract is used medicinally and in dietary supplements. To add to the overall toxicological dataset, Biokesum® () standardized extract was tested in a battery of guideline genotoxicity tests, namely the OECD Guideline 471 bacterial reverse mutation test, the OECD Guideline 473 mammalian cell chromosomal aberration test, and the OECD Guideline 487 micronucleus assay. The use of multiple standardized, validated assays increases the overall predictive value relative to individual assays and provides greater confidence in the test results. All three assays were negative, demonstrating that Biokesum® is not genotoxic.
An Optimized Probiotic Formulation Achieves Sustained Gut Microbiota Modulation and Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice.
Zhang H, Luan Y, Gao Q … +2 more , Zhang B, Hong W
J Med Food · 2025 Oct · PMID 40512571 · Publisher ↗
Although probiotic products are widely used to promote gut health, their long-term efficacy is often constrained by poor colonization and transient microbial modulation. To address these limitations, we developed and eva...
Although probiotic products are widely used to promote gut health, their long-term efficacy is often constrained by poor colonization and transient microbial modulation. To address these limitations, we developed and evaluated a probiotic beverage comprising -based strains ( LGD-0817, LDL-1708, LPM-0188, LEP-1208, subsp. RHM-1068, LPA-2088, BMC-2020, LAF-1387, and MCA-3066) formulated to optimize inter-strain synergy, enhance colonization efficiency, and improve long-term functionality. 16S rRNA sequencing conducted one week after treatment cessation revealed a marked enrichment of and -taxonomic groups to which administered probiotic strains belong-indicating durable colonization by the introduced strains. To further assess the functional relevance of this microbial shift, a dextran sulfate sodium-induced colitis model was employed. Probiotic intervention significantly improved disease outcomes, including increased colon length, reduced histopathological inflammation scores, enhanced goblet cell numbers, and lowered levels of pro-inflammatory cytokines (IL-6, Tumor Necrosis Factor-alpha (TNF-α), and Interleukin (IL)-1β). Collectively, these results underscore the efficacy of this formulation in achieving sustained modulation of the gut microbiota and alleviating intestinal inflammation, and support its potential for broader applications in gut health management.
Therapeutic and Biological Properties of White Garlic and Black Garlic Extracts ( L.): A Comparative Analysis.
Ben Hmad H, Sellami N, Ben Jemaa H … +1 more , Lasram MM
J Med Food · 2025 Aug · PMID 40512569 · Publisher ↗
Black garlic is a rich natural source of beneficial compounds and exhibits various biological activities. This study aimed to quantify flavonoids and polyphenols in white garlic and black garlic extracts, evaluate their...
Black garlic is a rich natural source of beneficial compounds and exhibits various biological activities. This study aimed to quantify flavonoids and polyphenols in white garlic and black garlic extracts, evaluate their antioxidant power, assess their antibacterial activity against specific pathogenic bacteria, and examine their antiproliferative effects on U87 glioblastoma cancer cells. The extraction was conducted using distilled water, methanol, and ethanol, and the quantification of bioactive compounds, antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl test, antibacterial activity, and antiproliferative activity using the MTT assay. The findings of this research showed significantly higher levels of phenolic acids and flavonoids in black garlic compared to raw garlic, with methanol extracts containing the highest concentration of flavonoids (2.33 mg quercetin equivalent/g DM) and polyphenols (63.25 mg ascorbic acid equivalent/g DM). Both white garlic and black garlic extracts displayed excellent antioxidant activity, while methanol extracts of black garlic exhibited the highest inhibition percentages (96.3% ± 0.36). Methanol extracts of black garlic also demonstrated effective antibacterial activity, while the antiproliferative effects were significant for all extracts on U87 glioblastoma cancer cells. These findings highlight black garlic as a promising source of compounds with antiproliferative, antibacterial, and antioxidant properties, although the concentration of bioactive compounds may vary among different extracts.
Antitumor Effect of Isoliquiritigenin via Mitochondrial Dysfunction and Oxidative Stress in Cholangiocarcinoma Cells.
Bae SH, Na H, Ahn D … +2 more , Lee HK, Choi KC
J Med Food · 2025 Sep · PMID 40512556 · Publisher ↗
Isoliquiritigenin (ISL), a bioactive phytochemical derived from the root of , is known to exert anticancer effects by modulating cancer cell functions, including proliferation, metastasis, and autophagy. However, the ant...
Isoliquiritigenin (ISL), a bioactive phytochemical derived from the root of , is known to exert anticancer effects by modulating cancer cell functions, including proliferation, metastasis, and autophagy. However, the anticancer effects of ISL on cholangiocarcinoma (CCA) are limited. In this study, we investigated the anticancer effects of ISL on CCA cells (SNU-478 and HuCCT-1). In SNU-478 cells, ISL decreased the expression of glucose-regulated protein 78 (GRP78). ISL was also found to significantly reduce cell viability and colony area of both SNU-478 and HuCCT-1 cells. Furthermore, ISL treatment significantly increased the population of apoptotic cells, as observed through Annexin V/propidium iodide (PI) staining. PI staining showed that the ISL treatment induced significant cell cycle arrest compared to the control group, indicating that ISL-induced decreases in cell viability are associated with cell cycle arrest and apoptosis. ISL treatment also resulted in a significant decline in the mitochondrial membrane potential when observed through JC-10 staining. Mitochondrial superoxide staining revealed that ISL significantly upregulated the generation of mitochondrial reactive oxygen species (ROS). Acridine orange staining indicated that the ISL treatment upregulated lysosomal membrane permeability, which could be related to autophagy. These results suggest that ISL affects the proliferation and apoptosis of CCA cells, which might be related to its regulatory role in various cellular functions, including mitochondrial dysfunction, ROS balance, and autophagy. This study provides empirical evidence that ISL could be a potential candidate for CCA therapy.
100% Fruit Juice Intake, Glucose Profile, and Uric Acid: A Systematic Review.
D'Elia L, Zarrella AF, Pizzulo IL … +3 more , Attanasio A, Rendina D, Sievenpiper JL
J Med Food · 2025 Aug · PMID 40512554 · Publisher ↗
Several studies suggest a favorable effect of 100% fruit juice (100%FJ) on cardio-metabolic risk. However, international dietary guidelines disagree on recommendations regarding fruit drink consumption, and the published...
Several studies suggest a favorable effect of 100% fruit juice (100%FJ) on cardio-metabolic risk. However, international dietary guidelines disagree on recommendations regarding fruit drink consumption, and the published European Food Safety Authority draft, based only on epidemiological data, suggested a direct relationship between 100%FJ intake and the development of type 2 diabetes mellitus (T2DM) and gout. Thus, we performed a systematic review of both randomized controlled trials (RCT) and prospective studies that assessed the relationship between 100%FJ intake, uric acid metabolism, and glucose profile. Concerning previous meta-analyses, no new prospective studies were identified for the evaluation of T2DM, and no studies were identified for the assessment of gout risk in the general population; conversely, 3 studies were detected for the assessment of gestational diabetes risk. Moreover, new RCTs were detected and included in an updated meta-analysis on glucose profile and uric acid. 100%FJ intake was associated with a decrease in uric acid, and neutral associations were found for different markers of glucose metabolism and the risk of gestational diabetes. In summary, this meta-analysis confirmed that there was no difference between 100%FJ intake and the comparator on glucose metabolism. It also showed that 100%FJ intake resulted in lower levels of uric acid than the comparator. In addition, the analysis indicated a neutral effect of 100%FJ on gestational diabetes. Finally, the systematic review confirmed the neutral effect of 100%FJ on T2DM and suggested the need for further studies to evaluate the risk of gout.
(Bonpl.) L.G. Lohmann: An Overview of Medicinal Plant Studies.
de Figueiredo-Muniz VRG, Biz AP, de Oliveira Sousa IM … +4 more , Igarashi Mafra L, Foglio MA, Mathias AL, Mafra MR
J Med Food · 2025 Aug · PMID 40474821 · Publisher ↗
(Bonpl.) L.G. Lohmann (synonym Verlot) has aroused the medical interest of researchers in recent years. The leaves of are used in folk medicine as anti-inflammatory, antimicrobial, for wound healing of skin or mucous m...
(Bonpl.) L.G. Lohmann (synonym Verlot) has aroused the medical interest of researchers in recent years. The leaves of are used in folk medicine as anti-inflammatory, antimicrobial, for wound healing of skin or mucous membranes, ulcers, intestinal colic, diarrhea, and blood disorders such as hemorrhage and anemia. Herein, we present a review of the scientific production on employing the SciVerse Scopus database to elucidate the research trends and perspectives in this field of science. To identify relevant publications, 95 research articles and 10 reviews were selected, totaling 105 publications, of which Brazilian researchers published 91. The research areas most covered in the publications were Pharmacology, Agricultural and Biological Sciences, with the keywords highlighting flavonoids chemical class as the most cited. The data collected provides an overview of the status of this species, with scarce research reports on the application of products. Furthermore, is also unexplored in Food Science and Technology, demonstrating new research opportunities.
Efficacy and Safety of Extract (LHF618) for Treating Allergic Rhinitis: A Randomized, Double-Blind, Placebo-Controlled, Multicenter Clinical Study.
Lee Y, Lee JK, Park J … +3 more , Kim N, Kang HR, Kwon Y
J Med Food · 2025 Aug · PMID 40468211 · Publisher ↗
Allergic rhinitis (AR) has become a global health concern due to its increasing prevalence, significantly impacting the quality of life. Traditional treatment options often provide only temporary symptom relief and may c...
Allergic rhinitis (AR) has become a global health concern due to its increasing prevalence, significantly impacting the quality of life. Traditional treatment options often provide only temporary symptom relief and may cause adverse effects, leading to the exploration of alternative therapies. This study evaluates the effectiveness and safety of standardized extract (LHF618) for alleviating symptoms of AR. Over a 4-week period, patients receiving LHF618 showed significant reductions in total nasal symptom scores, particularly for symptoms such as nasal itching, sneezing, and congestion, compared with the control group. Additionally, LHF618 led to a marked improvement in rhinitis control assessment test scores and a significant reduction in specific immunoglobulin E levels related to dust mite allergens. Importantly, no significant adverse effects were observed, indicating that LHF618 is a well-tolerated and safe option. These findings suggest LHF618 could be a promising natural alternative for managing AR, especially in cases related to dust mite allergies.
Phytochemical Properties of (-)-Epicatechin Promotes Bone Regeneration Inducing Osteogenic Markers Expression BMP2, SPARC, and RUNX2 in Mesenchymal Stem Cells .
Palma-Lara I, Calzada-Mendoza CC, Mera-Jiménez E … +4 more , Romero López E, Amaya-Espinoza JL, Parra-Barrera A, Gutiérrez-Iglesias G
J Med Food · 2025 Aug · PMID 40444298 · Publisher ↗
(-)-Epicatechin (EC), a bioactive compound found in the plant kingdom contained in beans from the cacao tree, and other vegetables, has high flavonoid content. Traditionally consumed as chocolate, presents various benefi...
(-)-Epicatechin (EC), a bioactive compound found in the plant kingdom contained in beans from the cacao tree, and other vegetables, has high flavonoid content. Traditionally consumed as chocolate, presents various benefits in human health. Many flavonoids have demonstrated therapeutic effects against osteoporosis by enhancing bone density; however, the specific impact of EC on bone regeneration remains unknown. Since bone regeneration involves a small group of responsive stem cells, we investigated the flavonoids' effects on key bone precursors like mesenchymal stem cells (MSCs). We used a conventional osteogenic medium alone or combined with different EC concentrations. Our study revealed that low concentrations of EC could enhance the expression of osteogenic genes when combined with low concentrations of osteogenic medium and induced the highest differentiation of human MSCs compared with pharmacological concentrations. So, the increase in the expression of osteoblastic marker genes such as bone morphogenetic protein 2, Runt-related transcription factor 2, and secreted protein acidic and cysteine rich agreed with an increase in cellular calcium deposits. Therefore, we conclude that the natural flavonol (-)-EC improved the expression of genes involved in bone regeneration and could potentially serve as an adjunctive therapy for bone loss diseases.
Beyond Performance: Creatine Delays the Onset of Fatigue and Augments Muscle Regeneration in Doxorubicin-Induced Myotoxicity.
Sharif S, Busekrus R, Jones B … +1 more , Hydock D
J Med Food · 2025 Jun · PMID 40407697 · Publisher ↗
Doxorubicin (Dox) impairs myogenic regulatory factor (MRF) expression and induces myotoxicity, and previous studies showed that creatine (Cr) supplementation before Dox treatment prevents forelimb grip (FG) force reducti...
Doxorubicin (Dox) impairs myogenic regulatory factor (MRF) expression and induces myotoxicity, and previous studies showed that creatine (Cr) supplementation before Dox treatment prevents forelimb grip (FG) force reduction and alleviates fatigue. However, Cr supplementation's effects on MRFs with Dox treatment are not well known. The effects of Cr on skeletal muscle function and MRFs 1, 3, and 5 days following Dox treatment are investigated. Male rats were randomly assigned to the control saline group (Con+Sal), control doxorubicin group (Con+Dox), standard Cr diet (2% Cr for 4 weeks) doxorubicin group (Cr1+Dox), or Cr loading diet (4% Cr for 1 week followed by 2% Cr 3 weeks) doxorubicin group (Cr2+Dox). After 4 weeks of feeding, Dox groups received 15 mg/kg Dox and Sal received saline as a placebo. At 1, 3, and 5 days postinjection, FG force and muscle function of the extensor digitorum longus (EDL) were measured. Myf-6, Myf-5, MyoD, and myogenin expression was analyzed using Western blotting. At 5 days postinjection, Con+Dox, Cr1+Dox, and Cr2+Dox groups had significantly lower FG force than Con+Sal ( < .05). EDL maximal twitch force of Con+Dox, Cr1+Dox, and Cr2+Dox groups was significantly lower than Con+Sal ( < .05) at 3 and 5 days postinjection. At 1 and 5 days postinjection, Cr alleviated Dox-induced fatigue in EDL muscle. At 1-day postinjection, Cr1+Dox, and Cr2+Dox had significantly higher Myf-6 and myogenin expression than the Con+Sal group, and Cr2+Dox group had significantly higher Myf-5 and MyoD compared with the Con+Sal group ( < .05). Cr supplementation attenuated fatigue and enhanced early muscle repair and regeneration in Dox-induced myotoxicity.
Epigallocatechin-3-Gallate and Glabridin Synergistically Inhibited Melanogenesis by Molecular Interaction and the MicroRNA-Microphthalmia-Related Transcription Factor Pathway.
Song J, Chen Z, Li T … +3 more , Xu W, Feng G, Ge J
J Med Food · 2025 Jun · PMID 40407219 · Publisher ↗
Epigallocatechin-3-gallate (EGCG) and glabridin (GLA) are two phytochemicals derived from homologous plants used for medicine and food that have demonstrated efficacy in inhibiting excessive production of melanin and pro...
Epigallocatechin-3-gallate (EGCG) and glabridin (GLA) are two phytochemicals derived from homologous plants used for medicine and food that have demonstrated efficacy in inhibiting excessive production of melanin and promoting skin whitening properties. However, it is unknown whether EGCG and GLA can synergistically inhibit melanogenesis. The aim of this study was to explore the effects of the combination of EGCG and GLA on the expression and activity of microphthalmia-related transcription factor (MITF) and to reveal the mechanism of the synergistic treatment with EGCG and GLA on excessive melanin deposition. This study used B16F10 cells to screen for the synergistic effect on melanin deposition and determined the best ratio of EGCG and GLA. Dual-luciferase reported gene assays were used to explore whether GLA and EGCG could regulate the mRNA level of MITF. Molecular docking and molecular dynamics simulation were used to explain the synergistic interaction of EGCG and GLA with MITF, and then Masson-Fontana and hematoxylin-eosin were used to observe the skin changes of mice. The results showed that the combination of EGCG and GLA could synergistically regulate MITF and thus inhibit melanogenesis by modulating microRNA expression. EGCG and GLA were also able to synergistically interact with MITF to inhibit excessive melanogenesis. [Figure: see text].
Flower Extract Retards Platelet-Derived Growth Factor-BB-Stimulated Proliferation and Migration in Vascular Smooth Muscle Cells by Inhibiting the MAPK/NF-κB Pathway and Matrix Metalloproteinase Expressions.
Hsuan CF, Kuo YT, Chang TH … +6 more , Chen YL, Houng HY, Chang N, Chang S, Chang CC, Houng JY
J Med Food · 2025 Sep · PMID 40376736 · Publisher ↗
Vascular smooth muscle cells (VSMCs) are vital to the structure of blood vessel walls. Under abnormal vascular conditions, VSMCs undergo a phenotypic transformation, leading to enhanced cell proliferation, migration, and...
Vascular smooth muscle cells (VSMCs) are vital to the structure of blood vessel walls. Under abnormal vascular conditions, VSMCs undergo a phenotypic transformation, leading to enhanced cell proliferation, migration, and matrix synthesis. This contributes to the development of vascular diseases such as atherosclerosis, arteriosclerosis, and restenosis. During this process, platelet-derived growth factor (PDGF)-BB is a key inducer of the VSMC phenotypic transformation. (L.) Medic flower (AMf) is known for its rich nutritional value and traditional medicinal uses. Its extract has been clinically used to treat kidney diseases, but its impact on VSMCs has not been documented. This study explored the inhibitory effects of AMf ethanol extract (AME), hot water extract (AMW), and supercritical CO extract (AMS), and their five indicator components (rutin, quercetin, isoquercitrin, myricetin, and hyperoside) on PDGF-BB-stimulated proliferation and migration using a rat aortic smooth muscle cell (RASMC) model. Both AME and AMS showed a significant dose-dependent inhibition of PDGF-BB-induced RASMC proliferation and migration, with AME being more effective than AMS. In contrast, AMW had no effect. The five indicator compounds also showed excellent inhibitory effects. AME treatment effectively reduced the phosphorylation of JNK, ERK, p38, and NF-κB, and downregulated the expressions of the migration-promoting factors and in PDGF-BB-stimulated RASMCs. These findings suggest that AME protects VSMCs by regulating the phosphorylation of the MAPK/NF-κB pathway and suppressing MMP expression. Consequently, AME may help prevent or slow the progression of vascular diseases.
Supplementation Containing Bromelain on the Side Effects of Oncological Treatment: Systematic Review.
Pereira IC, Passos RB, Viana CMC … +5 more , Mascarenhas IF, Nascimento GTM, Silva FCCD, Sousa JMCE, Torres-Leal FL
J Med Food · 2025 Jun · PMID 40372963 · Publisher ↗
Systemic therapies for the treatment of cancer collaborate to reduce cancer progression and have been used for decades. However, despite the clinical benefits, its long-term use is associated with toxicity, promoting imp...
Systemic therapies for the treatment of cancer collaborate to reduce cancer progression and have been used for decades. However, despite the clinical benefits, its long-term use is associated with toxicity, promoting important side effects that can compromise the quality of life. Enzyme supplementation has been pointed out as a therapeutic potential in several diseases. Bromelain is an enzyme complex that regulates pathways associated with inflammation. This review aims to evaluate the use of bromelain-containing supplements to improve the side effects of cancer treatment. This systematic review was developed in PubMed, Web of Science, and Cochrane Library, using the terms: Cancer AND Bromelain. 239 studies were retrieved, and only three met our objective. In general, it was possible to observe that supplementation was able to reduce side effects of adjuvant hormone therapy and chemotherapy, such as mucosal dryness, arthralgia, and peripheral neuropathy induced by chemotherapy.
Enhances Bone Growth and Microarchitecture in Rats: Potential Involvement of IGF-1 Signaling.
Park SH, Park J, Yoo JY … +3 more , Kim HS, Lee M, Kim OK
J Med Food · 2025 Jun · PMID 40372960 · Publisher ↗
This study investigated the effects of extract supplementation on bone growth and microstructural properties in male and female rats, with a particular focus on the JAK2/STAT5/IGF-1 signaling pathway. Three-week-old Spr...
This study investigated the effects of extract supplementation on bone growth and microstructural properties in male and female rats, with a particular focus on the JAK2/STAT5/IGF-1 signaling pathway. Three-week-old Sprague-Dawley rats were assigned to different groups receiving a normal diet (ND), growth hormone (GH), or varying doses of extract: low (L; 73 mg/kg body weight [bw]/day), medium (M; 146 mg/kg bw/day), and high (H; 292 mg/kg bw/day) for four weeks. The results demonstrated that the H group exhibited significant increases in femur and tibia lengths, trabecular and cortical bone mineral density, and growth plate thickness compared with the ND group. Furthermore, the H group demonstrated elevated serum and hepatic IGF-1 and IGFBP-3 levels, as well as enhanced phosphorylation of JAK2 and STAT5. The findings suggest that supplementation promotes longitudinal bone growth by stimulating growth plate activity and modulating the JAK2/STAT5-IGF-1 signaling pathway. This research indicates that extract could potentially be used as a natural therapeutic agent to support skeletal development and maintain bone health.
A Combined Turmeric and Allspice Supplement Improves Metabolic Health in Obese Rats by Reducing Carbohydrate and Lipid Absorption.
Barragan-Zuñiga J, Herrera MD, Simental-Mendía LE … +5 more , Zamilpa A, Morales-Ferra DL, Martínez-Aguilar G, Salas-Pacheco J, Gamboa-Gómez CI
J Med Food · 2025 Jun · PMID 40358049 · Publisher ↗
The objective of this study is to assess the impact of a combined turmeric ( L.) and allspice ( L. Merril) supplement (TAS), on obesity, hypertriglyceridemia, hyperglycemia, and insulin resistance through inhibition of c...
The objective of this study is to assess the impact of a combined turmeric ( L.) and allspice ( L. Merril) supplement (TAS), on obesity, hypertriglyceridemia, hyperglycemia, and insulin resistance through inhibition of carbohydrate and lipid absorption. In vitro assessments demonstrated that TAS inhibits key enzymes implicated in the carbohydrate and lipid absorption. Oral starch and lipid tolerance tests showed that combined supplement reduced lipid (∼47% TAS) and carbohydrate absorption (∼33%) compared to the negative control. In a 36-week diet-induced obesity model, intervention with TAS was found that reduced body weight gain (∼18%), lower triglycerides (∼35%), and fasting glucose levels (∼14%) compared to obese control. Furthermore, TAS-treated rats showed reduced oral glucose tolerance test values (∼25%), insulin levels (∼14%), and insulin resistance (∼14%). Our results suggest that TAS enhances carbohydrate and lipid absorption while effectively improving hypertriglyceridemia, hyperglycemia, and insulin resistance in obese rats. [Figure: see text].
In Silico and In Vivo Biological Evaluation of Extracts from Tunisian Desert Truffles ( and ) on Scopolamine-Induced Memory Impairment in Mice.
Smach MA, Hammami R, Zarrouk A … +4 more , Hafsa J, Charfeddine B, Limem K, Khadhri A
J Med Food · 2025 Jul · PMID 40335447 · Publisher ↗
The present work focuses on studying the effectiveness of truffle extract administration to enhance memory functions in a scopolamine-induced amnesia mice model. Major phytochemical constituents, gallic acid and myriceti...
The present work focuses on studying the effectiveness of truffle extract administration to enhance memory functions in a scopolamine-induced amnesia mice model. Major phytochemical constituents, gallic acid and myricetin, underwent studies using Autodock Vina and Discovery Studio visualizer software. Male mice were pretreated orally with either truffle extracts (400 mg/kg) or donepezil (3 mg/kg) for 21 days. Dementia was induced in mice by scopolamine (1 mg/kg) after 19 days of pretreatment and until day 28. The mice were divided into five experimental groups: (1) control; (2) model; (3) donepezil (1 mg/kg); (4) (400 mg/kg); and (5) (400 mg/kg). Mice were subsequently subjected to a battery of behavioral tests, including an open field locomotor activity assay, and the Morris water maze (MWM) test. Biochemical, histopathological analyses and molecular docking were performed. Chronic Scopolamine administration decreased learning and memory enhancement during the MWM test. A significant increase in time to find the hidden platform and escape was observed. Scopolamine increases acetylcholinesterase (AChE) activity and oxidative stress. Conversely, treatment with the and extracts (400 mg/kg orally administered) increased the memory of mice in the MWM task and suppressed scopolamine-induced oxidative damage. The memory enhancement effect was observed by the best docking scores of gallic acid and myricetin against AChE. This study reveals truffle's ameliorative effect on scopolamine-induced dementia in mice, possibly mediated by modulation of cholinergic activity and antioxidant activity. The docking of two major compounds, gallic acid and myricetin, predicted their potential as memory enhancers.
Therapeutic Effects of Edible Desert Truffle Specie () on Hepatogastric Toxicity Induced by Aspirin Treatment in Rats.
Said DB, Ali RB, Nouiri E … +3 more , Hammami MB, Elmay MV, El Aidli S
J Med Food · 2025 Jun · PMID 40333605 · Publisher ↗
Edible mushrooms (: Desert truffle specie) are a nutrient-dense source of flavonoids, proteins, fatty acids, minerals, and vitamins. The current study aimed to evaluate the curative effect of an aqueous extract of ascoc...
Edible mushrooms (: Desert truffle specie) are a nutrient-dense source of flavonoids, proteins, fatty acids, minerals, and vitamins. The current study aimed to evaluate the curative effect of an aqueous extract of ascocarps against aspirin-induced gastric, liver, and kidney injuries in rats. Hepatogastric disorders induced by aspirin at a dose of 10 mg/kg body weight (BW) treatment in rats and treated with aqueous extract of at different doses 25, 50, and 100 mg/kg BW). aqueous extract liquid chromatography-mass spectroscopy analysis showed several actives biomolecules such as quinic acid, syringic acid, O-coumaric acid, chlorogenic acid, caffeic acid, luteolin-7-O-glucoside, 4-O-caffeoylquinic acid, and vitamin C. The 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity revealed significant antioxidant activity (EC% = 0.6 mg/mL). Aspirin administration caused severe injuries with linear hemorrhagic lesions. The treatment with extract ameliorates gastric mucosal structure. The aspirin treatment caused significant increase of alanine aminotransferase and aspartate aminotransferase activities. In curative objective, extract significantly corrected the disturbance of liver parameters. Rats given aspirin showed altered liver architecture, apoptosis, and inflammation; in contrast, sections of liver tissue in the rats treated with extract at 25 mg/(kg·day) after aspirin administration showed normal liver architecture. A normal architecture was restored once the treatment dose was increased to 50 mg/kg. After 14 days of treatment with 100 mg/(kg·day) of extract, aspirin disruptions completely collapse with development of steatosis. On contrary, one week of rat's treatment by aspirin succeed by 2 weeks without any treatment, the histological examination of the kidney showed a dilated capillary and altered Bellini tubes. Normal renal architecture was observed at a dosage of 25 mg/(kg·day) of . Extract from restored the renal disturbances caused by aspirin.
Randomized Placebo Controlled Clinical Study of Dill () in Thyroiditis and Nodular Goiter Patients.
Destek S, Demirbolat İ, Yabacı A … +6 more , Kalemoglu M, Kartal M, Ersoy EY, Gul VO, Kayıran Z, Aysan ME
J Med Food · 2025 Jul · PMID 40329862 · Publisher ↗
Thyroid nodules are common and affect half of the general population by the age of 60 years. The cause is believed to be due to hypothyroidism, mutational changes, and autoimmunity leading to thyroid carcinoma. Dill, a t...
Thyroid nodules are common and affect half of the general population by the age of 60 years. The cause is believed to be due to hypothyroidism, mutational changes, and autoimmunity leading to thyroid carcinoma. Dill, a traditional herbal remedy, has been used to treat thyroid dysfunctions such as hyperthyroidism and hypothyroidism. This study evaluates the effects of dill () on patients with thyroiditis and nodular goiter. A study was conducted on patients with benign thyroid nodules. They were divided into two groups; placebo (=35) and dill group ( = 33). Dried and ground dill was put into hydroxypropyl methylcellulose capsules and patients were given three capsules per day for 90 days. Various tests were conducted at the beginning and end of the study, including thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), anti-thyroid peroxidase (anti-TPO), anti-thyroglobulin (anti-Tg), and C-reactive protein (CRP) analysis, and thyroid nodule dimensions were measured by ultrasound. Statistical analysis was performed. After 90 days of treatment with dill extract, patients showed significant reductions in TSH, FT4, anti-TPO, and CRP levels, as well as a decrease in thyroid nodule sizes as demonstrated by ultrasonography. The dill-treated group had significantly decreased TSH ( = .020), FT4 ( = .001), anti-TPO ( = .004), CRP ( = .003) levels, and nodule sizes ( = .001) at the beginning and end of the study. Compared to the control group, the dill group had significantly decreased TSH ( = .009), fT4 ( < .001), anti-TPO ( = .001), CRP ( < .001) levels and nodule sizes ( < .001), which reached normal values. suppressed inflammation of the thyroid gland, reduced nodule size, and normalized TSH levels. Dill supplementation should be recommended for the treatment of thyroid nodules in selected patients.
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