Tohoku J Exp Med
· 2026 Jun · PMID 40967811
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Polycystic ovary syndrome (PCOS) affects the health of many females of childbearing age. PCOS cannot be completely cured at present, so it is very important to study the pathogenesis. miR-126-3p expression was detected b...Polycystic ovary syndrome (PCOS) affects the health of many females of childbearing age. PCOS cannot be completely cured at present, so it is very important to study the pathogenesis. miR-126-3p expression was detected by RT-qPCR. The receiver operating characteristic (ROC) curve was employed to evaluate the diagnostic value. In human ovarian granulosa-like tumor cell line (KGN) cells, miR-126-3p and solute carrier family 7 member 5 (SLC7A5) were overexpressed or inhibited by transfection. The target of miR-126-3p was forecasted using the miRDB database. The correlation of the two in PCOS was proved using Pearson correlation analysis. miR-126-3p and SLC7A5 were knocked down in KGN cells to detect the function of both in PCOS. miR-126-3p was reduced and had a high diagnostic value in PCOS. In KGN cells, overexpressed miR-126-3p increased the proliferation and decreased the apoptosis, while inhibited miR-126-3p decreased the proliferation and increased the apoptosis. SLC7A5 in PCOS was increased and was negatively regulated by miR-126-3p. In KGN cells, the simultaneous overexpression of SLC7A5 and miR-126-3p decreased the proliferation and increased the apoptosis compared with the overexpression of miR-126-3p. miR-126-3p decreased and had a diagnostic value in PCOS. In KGN cells, SLC7A5 was the target gene of miR-126-3p, with which it exhibited a negative correlation.
Labor analgesia is commonly used in parturient women and its variable effect has been proven to be related to genetic factors. The P2RX7 rs7958311 polymorphism is closely associated with the occurrence and sensitivity of...Labor analgesia is commonly used in parturient women and its variable effect has been proven to be related to genetic factors. The P2RX7 rs7958311 polymorphism is closely associated with the occurrence and sensitivity of pain. This study focused on the correlation between the P2RX7 rs7958311 polymorphism and labor analgesia in primiparas. Two hundred full-term hospitalized primiparas who voluntarily requested labor analgesia were recruited in this study. P2RX7 rs7958311 genotyping was performed on all participants after genomic DNA extraction from blood samples. Ropivacaine combined with sufentanil was employed for labor analgesia. Evaluation was performed on pain scores, labor duration, and maternal and infant outcomes. Potential factors affected by rs7958311 polymorphism were assessed by logistic regression analysis. Obviously, reduced visual analog scale (VAS) scores were shown within 1 h after labor analgesia and at 2 h postpartum in GA and AA groups (P < 0.05) with lower VAS scores in the AA group than in the GA group (P < 0.01) at 2 h postpartum. The total and the first two stages of labor exhibited a shortened duration in the AA group (P < 0.05). Analysis of adverse reactions in primiparas and assessment of neonates identified a statistical difference in nausea/emesis (P = 0.005) among 3 groups. Logistic regression analysis demonstrated the effect of P2RX7 rs7958311 polymorphism on the VAS score at 2 h postpartum (OR = 0.386, 95% CI: 0.192-0.777, P = 0.008) and nausea/emesis (OR = 0.232, 95% CI: 0.071-0.762, P = 0.016) in primiparas. The P2RX7 rs7958311 polymorphism remarkably influenced the effect of labor analgesia in primiparas, especially the VAS score at 2 h postpartum and nausea/emesis.
Hypertension affects nearly 1/4 of the global population. LINC01123 is closely related to cancer and vascular smooth muscle cell function. Its role in hypertension remains unknown. This study aimed to investigate the exp...Hypertension affects nearly 1/4 of the global population. LINC01123 is closely related to cancer and vascular smooth muscle cell function. Its role in hypertension remains unknown. This study aimed to investigate the expression of LINC01123 in patients with hypertension and its damage to endothelial cells. In this study 149 healthy individuals and 149 hypertension patients participated. The expression of LINC01123 and miR-34a-5p was estimated by RT-qPCR. The relationship between LINC01123 and blood pressure, endothelial cell function and miR-34a-5p were analyzed by Pearson correlation analysis. Logistic regression analysis was employed to identify the potential risk factors of hypertension. The regulatory role of LINC01123 was explored in Human Umbilical Vein Endothelial Cells (HUVECs). In hypertension group, the significantly elevated LINC01123 was positively associated with systolic blood pressure (SBP) (r = 0.5182, P < 0.0001), diastolic blood pressure (DBP) (r = 0.5904, P < 0.0001), angiotensin II (Ang II) (r = 0.5614, P < 0.0001) and endothelin-1 (ET-1) (r = 0.6086, P < 0.0001) while a negative correlation was found with NO (r = --0.5534, P < 0.0001). LINC01123 (OR: 2.141, 95% CI: 1.339-3.424, P = 0.001) was identified as an independent risk factor of hypertension. LINC01123 knockdown contributed to improving the proliferation and migration of HUVECs while inhibiting its apoptosis. miR-34a-5p was a potential target of LINC01123. The significantly elevated LINC01123 was positively associated with blood pressure and vascular dysfunction in hypertension. LINC01123 was an independent risk factor for hypertension. LINC01123 knockdown facilitated the cell proliferation and migration while inhibiting apoptosis of HUVECs maybe via miR-34a-5p.
The expression of microRNA is likely to be closely associated with intrahepatic cholestasis of pregnancy (ICP). This study aimed to investigate the potential mechanisms underlying miR-940/RhoA-induced apoptosis in HTR8-S...The expression of microRNA is likely to be closely associated with intrahepatic cholestasis of pregnancy (ICP). This study aimed to investigate the potential mechanisms underlying miR-940/RhoA-induced apoptosis in HTR8-S/Vneo cells in ICP. In this study, 80 pregnant women diagnosed with ICP were recruited, with healthy pregnant women as controls. RT-qPCR was utilized to quantify the expression levels of miR-940 and RhoA. Cell proliferation was evaluated using the CCK-8 assay, while flow cytometry was employed to assess cell apoptosis. The dual-luciferase reporter assay confirmed the targeting relationship between miR-940 and RhoA. miR-940 expression was significantly elevated in the ICP group. ROC curve analysis demonstrated that miR-940 exhibited high diagnostic accuracy for ICP. Cell proliferation assays indicated that miR-940 negatively regulates cell proliferation. Apoptosis experiments revealed that low levels of miR-940 inhibited apoptosis. The dual-luciferase reporter assay confirmed that miR-940 targeted RhoA, establishing a negative correlation between them. The reversed experiment demonstrated that while the downregulation of miR-940 promoted cell proliferation and inhibited apoptosis, these effects were partially reversed when RhoA expression was suppressed. In conclusion, miR-940 played a crucial role in regulating cell proliferation and apoptosis, and it held potential as a therapeutic target for inhibiting the apoptosis of HTR8-S/Vneo cells in ICP.
Tohoku J Exp Med
· 2026 Jan · PMID 40967807
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Sanqi oral solution (SQ), a traditional Chinese medicine that is mainly composed of Astragalus membranaceus and Panax notoginseng, has been widely used for alleviating idiopathic membranous nephropathy, but its underlyin...Sanqi oral solution (SQ), a traditional Chinese medicine that is mainly composed of Astragalus membranaceus and Panax notoginseng, has been widely used for alleviating idiopathic membranous nephropathy, but its underlying molecular mechanism remains unclear. This study aims to investigate the effects of SQ on C5b-9-induced podocyte injury and explore the underlying mechanisms. After receiving 7-day intragastric administration of SQ (6.3 mL/kg/day), blood was harvested from the abdominal aorta of mice to prepare SQ-containing serum. MPC-5 cells were stimulated by the C5b-9 complex to construct an in vitro model of podocyte injury. CCK-8, flow cytometry, and lactate dehydrogenase (LDH) assays were employed to evaluate the effect of SQ-containing serum on podocyte injury. Immunofluorescence staining was performed to investigate the intracellular reactive oxygen species (ROS) levels. Western blotting was conducted to detect the expression of synaptopodin and podocin and activation of the endoplasmic reticulum (ER) stress/unfolded protein response (UPR) pathway. SQ-containing serum markedly ameliorates the podocyte injury induced by C5b-9, as evidenced by the increased cell viability, upregulated expression level of synaptopodin and podocin, and reduced cell apoptosis and LDH activity in C5b-9-treated MPC-5 cells. Additionally, SQ-containing serum significantly suppresses ROS levels in injured podocytes. Mechanically, SQ-containing serum could significantly block C5b-9-induced intracellular activation of the ER stress/UPR pathway in podocytes. After introducing 4-PBA, an ER stress inhibitor, the protection of SQ-containing serum was hardly observed on C5b-9-treated MPC-5 cells. SQ-containing serum ameliorates podocyte injury by suppressing oxidative stress and intracellular activation of the ER stress/UPR pathway.
Li R, Li N, Lv C
… +4 more, Xu X, Song J, Bai R, Hua X
Tohoku J Exp Med
· 2026 Jun · PMID 40930787
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Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a rare arrhythmia syndrome often caused by biallelic variants in the TECRL gene. This study reviews a Chinese family with two generations and six members af...Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a rare arrhythmia syndrome often caused by biallelic variants in the TECRL gene. This study reviews a Chinese family with two generations and six members affected by CPVT. The proband experienced syncope, loss of consciousness after exercise, and recurrent seizures, with electrocardiograph showing prolonged QTc interval and extreme ventricular tachycardia. Her elder sister died of syncope and cardiac arrest after exercise. Genetic analysis identified compound heterozygous variants in the TECRL gene: c.587G>A (p.Arg196Gln) and c.868C>T (p.Pro290Ser), inherited from each parent. Other family members without symptoms only carried one heterozygous variant. The p.Arg196Gln variant was previously reported, but p.Pro290Ser was a novel variant. Molecular dynamics simulations and Western blot analysis suggested that the structural stability and expression levels of the mutant protein were altered. This study underscores the significance of molecular diagnostics in patients experiencing syncope or cardiac arrest triggered by environmental changes or exercise, as early detection can facilitate personalized care and targeted treatment.
Immunity and inflammation contribute to the development of recurrent spontaneous abortion (RSA); tumor necrosis factor inhibitor (TNFi) is a well-known anti-inflammation treatment for immune diseases. This study aimed to...Immunity and inflammation contribute to the development of recurrent spontaneous abortion (RSA); tumor necrosis factor inhibitor (TNFi) is a well-known anti-inflammation treatment for immune diseases. This study aimed to investigate the effect of TNFi on pregnancy outcomes and tolerance in RSA patients. This retrospective, observational study analyzed 115 RSA patients. Forty-three patients received TNFi plus intravenous immunoglobin (IVIG) and low molecular weight heparin (LMWH) treatment (the TNFi group), whereas another 72 patients received IVIG plus LMWH or LMWH alone treatment (the Control group). TNFi agents included certolizumab, etanercept, and adalimumab. The live birth rate was greater in the TNFi group than in the Control group (72.1% vs. 45.8%, P = 0.006), whereas the premature delivery rate tended to be lower in the TNFi group vs. the Control group but did not reach statistical significance (9.7% vs. 24.2%, P = 0.123). Multivariable logistic regression revealed that treatment (TNFi vs. Control) was independently correlated with greater live birth achievement (P = 0.001, odds ratio = 5.470); furthermore, treatment (TNFi vs. Control) tended to be independently associated with a reduced premature delivery risk (P = 0.055, odds ratio = 0.178). The type of labor (P = 0.321), neonatal birth weight (P = 0.159), and newborn Apgar score (P = 0.233) did not differ between the TNFi group and the Control group. Furthermore, adverse events, including rash, gestational hypertension, abnormal liver function index, ecchymosis, fever, and abnormal renal function index were not different between the TNFi and Control groups (all P > 0.05). TNFi improves live birth rate and tends to reduce premature delivery risk in RSA patients, providing a potential treatment option.
Song J, Zhang J, Feng Q
… +3 more, Huang Y, Huang Y, Lian Y
Tohoku J Exp Med
· 2026 May · PMID 40803881
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Resolvin D1 (RvD1) is involved in the pathology of acute myocardial infarction (AMI) by regulating inflammation and atherosclerosis. This study aimed to delve into the potential of RvD1 as a diagnostic marker for AMI. A...Resolvin D1 (RvD1) is involved in the pathology of acute myocardial infarction (AMI) by regulating inflammation and atherosclerosis. This study aimed to delve into the potential of RvD1 as a diagnostic marker for AMI. A total of 149 AMI patients who underwent percutaneous coronary intervention (PCI) and 30 healthy controls (HC) were enrolled in this study. Blood RvD1 was detected by enzyme-linked immunosorbent assay in AMI patients at baseline and after PCI. Blood RvD1 was also detected in HC after enrollment. The median [interquartile range (IQR)] level of RvD1 was lower in AMI patients [1.04 (0.69-1.56) ng/mL], ST-segment elevation myocardial infarction (STEMI) patients [0.95 (0.61-1.28) ng/mL], and non-STEMI patients [1.47 (0.96-2.69) ng/mL] than in HC [2.56 (1.42-4.95) ng/mL] (all P < 0.001). RvD1 was also decreased in STEMI patients compared with non-STEMI patients (P < 0.001). RvD1 had a good ability to discriminate AMI patients from HC [area under the curve (95% confidence interval): 0.815 (0.739-0.892)]. RvD1 exhibited acceptable diagnostic performance for AMI when the cutoff values ranged from 1.1 to 2.5 ng/mL, with Youden indexes ranging from 0.406 to 0.498. The Youden index peaked at 0.498 when the cutoff value of RvD1 was 1.2 ng/mL, with a sensitivity of 0.631 and a specificity of 0.867. RvD1 was negatively related to triglycerides (P = 0.003) and cardiac troponin I (P = 0.012) in AMI patients. RvD1 was increased after PCI compared to its level at baseline in AMI patients (P < 0.001). RvD1 is associated with a lower AMI risk, and its level is increased after PCI.
Tohoku J Exp Med
· 2026 May · PMID 40803880
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The decline in lower limb muscle strength contributes to reduced physical activity and limited quality of life. While lower-intensity physical activities are widely recommended for sedentary adults, they are often insuff...The decline in lower limb muscle strength contributes to reduced physical activity and limited quality of life. While lower-intensity physical activities are widely recommended for sedentary adults, they are often insufficient to maintain strength. Additional muscle-strengthening exercises are not widely adopted due to time constraints and physical constraints. Rei-ho, a traditional practice of the Japanese samurai, is believed to enhance lower limb strength through slow, self-weight-bearing movements incorporated into daily activities like standing, sitting, and walking. We conducted a randomized controlled trial to examine the effect of brief Rei-ho-based training on knee extension strength. Thirty-four healthy adults with no prior experience in Rei-ho were randomly assigned to either a Rei-ho-based training group (n = 17) or a control group (n = 17). The training group performed a 5-minute Rei-ho-based session once per day, for at least 4 days per week, over a period of 3 months. Each session consisted of 10 repetitions of sit-to-stand from a chair and 10 repetitions of squatting down and standing up, increasing to 12 repetitions from the third week. Control group participants continued their normal daily routines without any exercise intervention. Isometric knee extension strength was measured before and after the training period. After 3 months, the Rei-ho-based training group showed a significant increase in knee extension strength, with an average gain of 25.9%, compared to only 2.5% in the control group. Our findings suggest that Rei-ho-based training, easily integrated into daily life, is an effective method for improving knee extension strength in healthy adults.
Elevated histone lactylation is crucial in the initiation and progression of various cancers. Although its role in breast cancer remains unconfirmed, the increased lactate release from enhanced glycolysis in triple-negat...Elevated histone lactylation is crucial in the initiation and progression of various cancers. Although its role in breast cancer remains unconfirmed, the increased lactate release from enhanced glycolysis in triple-negative breast cancer (TNBC) suggests a potential link. In order to investigate the effect of histone lactylation on TNBC, we detected the lactate production and histone lactylation levels. Chromatin immunoprecipitation (ChIP) assay was used to confirm that minichromosome maintenance complex component (MCM) 7 is a downstream target. A variety of experimental techniques including, cell viability, colony formation assay, apoptosis assay, and transwell assay were used to describe the cell proliferation and migration ability. Histone lactylation levels were increased in TNBC tissues. Inhibiting lactate production suppressed the proliferation and migration of TNBC cells and histone lactylation in TNBC cells. Histone lactylation modulated MCM7 expression and translation, and MCM7 participated in histone lactylation regulation of TNBC cell function. These data show that enhanced glycolysis in TNBC cells results in increased lactate production, directly contributing to histone lactylation and subsequent activation of MCM7 promoter. Our results highlight that targeting the feedback loop involving glycolysis, lactate production, histone lactylation, and MCM7 transcription could be a therapeutic strategy for TNBC by inhibiting cell proliferation and migration.
Ebana H, Kurosawa S, Oishi R
… +2 more, Obara S, Inoue S
Tohoku J Exp Med
· 2026 Jun · PMID 40707200
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Low-dose dexamethasone is widely used in perioperative settings for its antiemetic and analgesic effects. The present study aimed to investigate the impacts of low-dose dexamethasone on cell kinetics, cytokine production...Low-dose dexamethasone is widely used in perioperative settings for its antiemetic and analgesic effects. The present study aimed to investigate the impacts of low-dose dexamethasone on cell kinetics, cytokine productions and cytotoxic activity of natural killer (NK) cells in lymphoid organs. Female Balb/c mice were intraperitoneally administered normal saline (Group N), low-dose dexamethasone (0.1 mg/kg) (Group L), or high-dose dexamethasone (20 mg/kg) (Group H). Measurement of cell counts of thymocytes and splenocytes, and phenotypic analyses of the lymphocytes and the cytotoxicity of NK cells in the splenocytes using flow cytometry were performed. The production of cytokines in the splenocytes ex vivo was measured by ELISA. There was a marked decrease in the number of lymphocytes in Group H, while the number of splenocytes was significantly increased in Group L compared to Group N at 24 hours after administration (p = 0.019). The production of IFN-γ was dose-dependently decreased by dexamethasone. The proportions of double-positive thymocytes and CD4 splenocytes were reduced in Group H, but there were no significant changes in lymphocyte subsets between Groups N and L until 48 hours after administration. The cytotoxicity of NK cells in Group L was increased compared to that in Group N at 24 and 48 hours (p = 0.008 and p = 0.018, respectively). Low-dose dexamethasone augmented immune cell mobilization and upregulated cytotoxicity of NK cells while suppressing cytokine production. These results suggested that low-dose dexamethasone have conflicting immunological effects and transiently enhances innate immunity such as NK cell function.
Luo L, Luo M, Xie L
… +3 more, Tang M, Luo M, Cui J
Tohoku J Exp Med
· 2026 Mar · PMID 40670092
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Women with diminished ovarian reserve (DOR) have reduced fertility, but the mechanisms regulating ovarian function remain unclear. This study aims to explore the mechanism by which FOXO1 regulates ovarian granulosa cell...Women with diminished ovarian reserve (DOR) have reduced fertility, but the mechanisms regulating ovarian function remain unclear. This study aims to explore the mechanism by which FOXO1 regulates ovarian granulosa cell function. The ovarian granulosa cell line KGN was cultured and transfected with FOXO1 and LHX8 vectors. Cell proliferation and apoptosis were assessed using CCK-8 and flow cytometry, respectively. mtROS content was determined by immunofluorescence. ATP was detected using an assay kit. E2 was detected by ELISA. ChIP was used to detect the binding of FOXO1 to the LHX8 promoter. Luciferase reporter genes were used to analyze the regulation of FOXO1 on LHX8 promoter activity. RT-qPCR was performed to measure the expression of FOXO1 mRNA, LHX8 mRNA, ND1, and β-globin. Western blotting was used to detect FOXO1, LHX8, and aromatase proteins.FOXO1 knockdown or LHX8 overexpression promoted proliferation, inhibited apoptosis, reduced mitochondrial ROS, and increased ATP, mtDNA, E2, and aromatase levels in KGN cells. FOXO1 overexpression or LHX8 knockdown produced the opposite regulatory effects. FOXO1 bound to the LHX8 promoter and reduced its activity. LHX8 overexpression eliminated FOXO1-induced KGN cell damage. In conclusion, FOXO1 reduces viability and promotes apoptosis in granulosa cells by inhibiting LHX8 transcription to induce mitochondrial dysfunction, which may contribute to DOR.
Liu L, Gao Y, Ao L
… +8 more, Yang C, Zhao W, Deng C, Zhu Q, Chen Y, Lan Q, Hao Q, Wang Y
Tohoku J Exp Med
· 2026 Feb · PMID 40670091
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Vascular endothelial dysfunction plays a critical role in the development of preeclampsia (PE). Secreted protein acidic and cysteine rich like 1 (SPARCL1) plays a role in regulating angiogenesis, yet its role in PE remai...Vascular endothelial dysfunction plays a critical role in the development of preeclampsia (PE). Secreted protein acidic and cysteine rich like 1 (SPARCL1) plays a role in regulating angiogenesis, yet its role in PE remains unclear. This study investigates the involvement of SPARCL1 in the development of PE. Placental tissues from healthy volunteers and patients with PE were collected to detect the expression of SPARCL1. Human umbilical vein endothelial cells (HUVECs) cultured under hypoxic conditions were utilized to investigate the role of SPARCL1 in PE. The biological behaviors of the cells were examined through cell functional assay. The expressions of DLL4/NOTCH1/HES1/VEGF proteins as well as apoptosis-related proteins were evaluated by western blot. The effects of SPARCL1/DLL4 on hypoxia-induced HUVECs were verified via rescue experiments. SPARCL1 was upregulated in placental tissues of PE patients and hypoxia-induced HUVECs. In hypoxia-induced HUVECs, shSPARCL1 facilitated the proliferative, migratory, invasive, and tube-forming capabilities yet inhibited apoptosis. ShSPARCL1 upregulated the protein levels of VEGF, HES1, NOTCH1 and DLL4. However, above-mentioned effects were all reversed by shDLL4. SPARCL1 may influence the proliferative, migratory, invasive, and tube-forming capabilities of hypoxia-induced HUVECs by regulating the DLL4/NOTCH1 axis, thereby facilitating the progression of PE.
Yang W, Zeng R, Song J
… +6 more, Ma N, Zhu W, Fu T, Zhang T, Li F, Wu B
Tohoku J Exp Med
· 2026 Jun · PMID 40670090
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Drug resistance affects the therapeutic effect of adriamycin on gastric cancer (GC). Therefore, this study aimed to explore the mechanism of basic leucine zipper (bZIP) transcription factor ATF-like 2 (BATF2) on adriamyc...Drug resistance affects the therapeutic effect of adriamycin on gastric cancer (GC). Therefore, this study aimed to explore the mechanism of basic leucine zipper (bZIP) transcription factor ATF-like 2 (BATF2) on adriamycin resistant (ADR) in GC cells and the involvement of mitochondrial fission in this process. A BATF2-overexpressed lentiviral vector was transfected into SGC7901/ADR cells, and effects of elevated BATF2 expression on the sensitivity of SGC7901 cells to adriamycin was observed by Transwell assays. The changes in mitochondrial morphology, fission marker expressions, and ATP levels after BATF2 overexpression were evaluated using Mito-Tracker Red staining, laser confocal, western blot and a detection kit. A ERK agonist was added to observe the effect of BATF2 overexpression on ERK phosphorylation levels. Co-immunoprecipitation (Co-IP) was used to confirm protein interactions between BATF2 and p53. PFT-α was adopted to observe the effects of p53 inhibition on BATF2-mediated ADR. In vitro experiments revealed that BATF2 overexpression restored drug sensitivity to adriamycin in SGC7901 cells and inhibited Drp1-dependent mitochondrial fission. Meanwhile, BATF2 overexpression also achieved the reversal of ADR in GC cells by inhibiting the phosphorylation level of ERK. P53 is an upstream regulator of ERK and interacts with BATF2 at the protein level. The suppression of p53 can significantly attenuated the inhibition of BATF2 overexpression on ADR, ERK phosphorylation, and Drp1-dependent mitochondrial fission in SGC7901/ADR cells. To conclude, BATF2 targets p53 and reverses drug resistance to adriamycin in GC cells by inhibiting ERK phosphorylation levels and downstream Drp1-dependent mitochondrial fission.
Presurgical infant orthopedics (PSIO) has been used worldwide to improve orofacial deformations due to cleft lip and palate before primary surgeries. However, their effectiveness remains controversial. This retrospective...Presurgical infant orthopedics (PSIO) has been used worldwide to improve orofacial deformations due to cleft lip and palate before primary surgeries. However, their effectiveness remains controversial. This retrospective study examined the effect of PSIO treatment on the orofacial morphology of patients with non-syndromic unilateral cleft lip and palate (UCLP) up to 5 years of age. The study included 22 patients with UCLP who were treated and followed up from birth to 5 years of age. The patients were divided into two groups. The PSIO group consisted of 11 patients, while the control group consisted of 11 patients who were not treated with PSIO. Maxillary dental casts obtained from birth to 5 years of age were analyzed three-dimensionally. Furthermore, 5-Year-Olds' Index evaluation was performed, along with cephalometric and facial morphometric analyses. The analyses were performed on dental casts, lateral cephalograms, and frontal facial photographs at 5 years of age, respectively. The PSIO group showed significantly superior alveolar cleft width, anterior palatal depth, and posterior palatal depth, as well as less lateral deviation of the incisor point before cheiloplasty than the control group. However, no significant differences were observed between the two groups in terms of occlusion, craniofacial form, or lip morphology at 5 years of age. The present results suggest that PSIO treatment may improve deformations of the maxillary alveolus and palate before cheiloplasty, thereby simplifying surgery. In contrast, PSIO had no long-term effects on the orofacial morphology of patients with UCLP, aged up to 5 years.
Tanaka Y, Konta T, Ono H
… +2 more, Ueno Y, Sakurada K
Tohoku J Exp Med
· 2026 Jun · PMID 40634071
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Iron is required for the synthesis of neurotransmitters that influence emotion and behavior. This study examined the relationship between iron intake and mental health in older adults using data from the Yamagata Cohort...Iron is required for the synthesis of neurotransmitters that influence emotion and behavior. This study examined the relationship between iron intake and mental health in older adults using data from the Yamagata Cohort study, which included a total of 6,184 individuals (3,256 men and 2,928 women) aged 65 years or above. Mental health was evaluated using the Kessler 6 scale (K6). Participants were divided into two groups based on the K6 score: those with a score < 9 and those with a score ≥ 9. Based on iron intake, male participants were divided into two groups: those with an iron intake of < 6 mg/day or more; female participants were classified as those with an iron intake of < 5 mg/day or more. In logistic regression analysis, after adjusting for 10 factors, including age, hypertension, diabetes, dyslipidemia, alcohol consumption history, smoking history, marital status, total energy intake per day, body mass index, and hemoglobin level, the number of men with suspected depression was significantly higher among those with an iron intake of < 6 mg/day. No significant differences were observed among women. These results suggest a potential association between increased iron intake and improved mental health among male older adults.