Tohoku J Exp Med
· 2026 Mar · PMID 41192874
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Currently, the pathogenesis of sepsis has not been fully elucidated. This study aimed to explore the role of cytochrome P450 oxidoreductase (POR) in this disease. Specifically, two-sample Mendelian randomization, multiva...Currently, the pathogenesis of sepsis has not been fully elucidated. This study aimed to explore the role of cytochrome P450 oxidoreductase (POR) in this disease. Specifically, two-sample Mendelian randomization, multivariate Mendelian randomization, and summary data-based Mendelian randomization methods were employed to assess the genetic impact of peripheral POR protein and POR genes from multiple tissue sources on the risk of streptococcal sepsis (ICD-10 A40) and other sepsis (ICD-10 A41). Subsequently, a mouse model of sepsis (based on lipopolysaccharide) was constructed, and the expression of POR was specifically upregulated and downregulated through AAV9-mediated overexpression and shRNA interference, respectively. Various methods were used to evaluate physiology, liver and kidney function, peripheral inflammation, and oxidative stress levels of the mice. The results of Mendelian randomization analysis showed that elevated levels of peripheral POR protein and POR genes from multiple tissues were associated with a reduced risk of other sepsis, but not with the risk of streptococcal sepsis. Animal experiments demonstrated that specific upregulation of POR expression improved the moving distance, heart rate, blood pressure, liver and kidney functions, peripheral inflammation, and oxidative stress levels in septic mice, while specific downregulation of POR expression exacerbated the above indicators. In conclusion, evidence from both genetic and animal studies indicates that POR may play an important protective role in the occurrence and development of sepsis (excluding streptococcal sepsis).
Okada N, Saito R, Cha K
… +9 more, Suzuki S, Ogasawara H, Shono Y, Matsumura M, Sasaki K, Fujio A, Tokodai K, Unno M, Kamei T
Tohoku J Exp Med
· 2026 Jun · PMID 41192873
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Intra-abdominal complications related to spinal surgery are uncommon but may result in serious morbidity. Prone positioning, often used in scoliosis correction procedures, has been implicated in compression-induced injur...Intra-abdominal complications related to spinal surgery are uncommon but may result in serious morbidity. Prone positioning, often used in scoliosis correction procedures, has been implicated in compression-induced injuries of visceral organs, particularly in patients with anatomical deformities such as thoracic asymmetry and lumbar lordosis. We report a rare case of concurrent liver injury, pancreatic damage, and renal dysfunction following posterior spinal fusion in a 12-year-old male with idiopathic scoliosis. Despite intraoperative decompression precautions and stable hemodynamics, the patient developed acute abdominal symptoms and elevated hepatic, pancreatic enzymes, and creatinine indicating renal damage on postoperative day 1. Imaging revealed a liver contusion, ascites, and reduced enhancement of the left kidney, suggestive of organ compression. Renal findings were attributed to venous congestion likely caused by compression of the left renal vein between the aorta and the superior mesenteric artery, resembling nutcracker syndrome. Conservative management led to rapid clinical improvement, with full recovery observed by postoperative day 18. This case highlights the importance of recognizing position-related organ injury during scoliosis surgery. Awareness of risk factors-including low body mass index and skeletal deformities-and early assessment of blood flow are essential for accurate diagnosis and appropriate management. Preoperative simulation of prone positioning may help mitigate the risk in anatomically vulnerable patients.
Khaing PT, Han YMZ, Zaww K
… +8 more, Kyaw MS, Thwin KM, Orui M, Kunii Y, Hamaie Y, Kyaw SS, Tsuboi M, Egawa S
Tohoku J Exp Med
· 2025 Nov · PMID 41161774
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On March 28, 2025, a 7.7-magnitude earthquake struck central Myanmar (GLIDE #EQ-2025-000043-MMR), compounding the country's existing political instability, economic fragility, and infrastructural weaknesses. Although sei...On March 28, 2025, a 7.7-magnitude earthquake struck central Myanmar (GLIDE #EQ-2025-000043-MMR), compounding the country's existing political instability, economic fragility, and infrastructural weaknesses. Although seismic events have recurred throughout history, limited knowledge exists regarding how working-age adults (18-60), who play a central role in recovery, mobilize personal, cultural, and community resources to cope with adversity and reconstruct their lives. This is a protocol clarification for a remote access qualitative study using semi-structured interviews with 30 purposively sampled participants, half from heavily impacted zones (Sagaing, Mandalay) and half from nearby regions experiencing secondary disruption (Yangon). Using a remote system, interviews will be audio-recorded, transcribed verbatim, and analyzed thematically following Graneheim and Lundman's approach. This study aims to identify key coping strategies, including social support networks, spiritual practices, and local initiatives of working-age adults in Myanmar, and to compare how exposure severity shapes adaptive responses. Anticipated themes based on existing disaster and resilience literature include community-led resilience, hope through faith, and resource-sharing practices. Findings are expected to offer in-depth, culturally grounded insights that can inform disaster recovery programs, psychosocial support services, and policy frameworks aimed at strengthening resilience in Myanmar and similar low-resource, low-accessibility, crisis-affected settings.
The relationship between Gestational Diabetes Mellitus (GDM) and Retinopathy of Prematurity (ROP) is not fully understood, but both conditions may share critical molecular biomarkers. This study integrated datasets to id...The relationship between Gestational Diabetes Mellitus (GDM) and Retinopathy of Prematurity (ROP) is not fully understood, but both conditions may share critical molecular biomarkers. This study integrated datasets to identify genes associated with GDM and ROP. Weighted Gene Co-expression Network Analysis (WGCNA) was employed to construct networks and identify gene modules related to GDM and ROP. Three machine learning models were utilized to filter for core co-morbid genes. Immune infiltration analysis was performed to investigate the correlations of these genes with immune cells. Finally, a diagnostic nomogram model was constructed based on the expression levels of the three core co-morbid genes, and Receiver Operating Characteristic (ROC) curve analysis was conducted to assess its diagnostic capabilities. We identified 16 co-morbid genes associated with GDM and ROP. Through machine learning model filtering, chorionic gonadotropin beta subunit 7 (CGB7), MYCN upstream transcript (MYCNUTR), and G protein-coupled receptor 32 (GPR32) were confirmed as core genes. These genes exhibited significantly elevated expression levels in patients with GDM and ROP, positively correlating with activated cytotoxic T lymphocytes (CD8 T) cells and negatively correlating with central memory CD8 T cells. The diagnostic nomogram model achieved an Area Under the Curve (AUC) value of 0.913 in the GDM training set and 0.875 in the ROP training set. This study reveals a potential shared critical role for CGB7, MYCNUT, and GPR32 in the interplay between GDM and ROP. The constructed diagnostic nomogram model shows promising predictive capability.
Bian M, Du X, Fan X
… +4 more, Wei Y, Li B, Pang L, Han J
Tohoku J Exp Med
· 2026 Jan · PMID 41125359
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Septic lung injury is a severe clinical problem with high mortality, and oxidative stress plays a critical role in its pathogenesis. This research focused on investigating how IGF2BP3 stabilizes CCL25 mRNA and its subseq...Septic lung injury is a severe clinical problem with high mortality, and oxidative stress plays a critical role in its pathogenesis. This research focused on investigating how IGF2BP3 stabilizes CCL25 mRNA and its subsequent effects on cellular oxidative stress and septic lung injury. An in vitro cell injury model was established using LPS. The CCK-8 assay, flow cytometry and ELISA were employed to evaluate cell viability, death and inflammatory cytokine levels respectively. ROS accumulation, MDA content and antioxidant enzyme activities (SOD and GSH-Px) were quantified. Analysis of IGF2BP3 and CCL25 expression was conducted through qPCR and Western Blot. To confirm the interaction between IGF2BP3 and CCL25 mRNA, RIP and RNA pull-down were conducted. CCL25 mRNA stability was assessed following actinomycin D administration. Sepsis-caused lung injury model was created using CLP in mice. Further validation of CCL25's involvement in septic lung injury was conducted through qPCR, Western Blot, HE, ELISA and measurement of oxidative stress indicators. After LPS treatment, the cell viability was significantly decreased and the cell death, inflammation as well as oxidative stress were induced, the expressions of IGF2BP3 as well as CCL25 were markedly increased. Knockdown of CCL25 alleviated cellular oxidative stress and cellular damage caused by LPS. In addition, IGF2BP3 bound CCL25 and stabilized CCL25 mRNA, thus regulating LPS-induced cellular oxidative stress and cellular damage. In vivo, knockdown of CCL25 alleviated oxidative stress and inflammatory injury of lung tissue. CCL25 stabilized by IGF2BP3 could promote septic lung injury by inducing cellular oxidative stress.
Tohoku J Exp Med
· 2026 May · PMID 41125358
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Live Combined Bacillus subtilis and Enterococcus faecium (LCBE) is a pioneer probiotic restoring intestinal microecological balance by improving anaerobic environment. This randomized controlled trial (RCT)-based meta-an...Live Combined Bacillus subtilis and Enterococcus faecium (LCBE) is a pioneer probiotic restoring intestinal microecological balance by improving anaerobic environment. This randomized controlled trial (RCT)-based meta-analysis intended to comprehensively explore the efficacy of LCBE in treating and preventing antibiotic-associated diarrhea (AAD) in pediatric and adult patients undergoing antibiotic treatment. A comprehensive search was performed in Cochrane Library, PubMed, Web of Science, China Science and Technology Journal Database, Wan Fang, and China National Knowledge Infrastructure from establishment to December 2, 2024. The treatment efficacy (reflected by total effective rate and time to relief of symptom) and preventive efficacy (reflected by incidence of diarrhea) for AAD were assessed. Thirty-three studies involving 2919 cases with LCBE plus symptomatic therapy (experimental group) and 2781 cases with symptomatic therapy alone (control group) were included. Experimental group reached a higher total effective rate [odds ratio (OR) {95% confidence interval (CI)}: 6.541 (4.850, 8.822), P < 0.001], shorter time to relief of symptom [mean difference (95% CI): -1.798 (-2.364, -1.232) days, P < 0.001], and lower incidence of diarrhea [OR (95% CI): 0.275 (0.234, 0.324), P < 0.001] vs. control group. Subgroup analyses based on LCBE granule and capsule form showed similar results. All studies were high-quality and there was no publication bias reflected by ROB 2.0 tool and Begg's test analyses, respectively. Sensitivity analyses indicated high robustness. In conclusion, LCBE increases total effective rate (OR = 6.541) for AAD treatment and decreases diarrhea risk (OR = 0.275) for AAD prevention, which may be a promising probiotic for pediatric and adult patients undergoing antibiotic treatment.
Tohoku J Exp Med
· 2026 Jun · PMID 41093593
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Osteoglophonic dysplasia (OD) is a rare skeletal disorder marked by craniosynostosis, craniofacial anomalies, and abnormal bone development, primarily caused by FGFR gene mutations. We present the case of a 26-year-old C...Osteoglophonic dysplasia (OD) is a rare skeletal disorder marked by craniosynostosis, craniofacial anomalies, and abnormal bone development, primarily caused by FGFR gene mutations. We present the case of a 26-year-old Chinese female, who has been followed since age 11 for recurrent central giant cell granulomas (CGCG) and non-ossifying fibroma affecting her jaws and lower limbs. While her craniofacial and limb features have been described in two earlier publications, this report provides a detailed account of the aggressive and recurrent maxillary CGCG that resulted in significant clinical morbidity. Despite treatment with Alendronate targeting femoral lesions, the patient sustained a pathological fracture, resulting in long-term wheelchair dependency. Additionally, the maxillary lesion persisted and extended into the ethmoid sinus, causing recurrent epistaxis and anemia that required multiple surgical interventions. A multidisciplinary team initiated treatment with Denosumab, a monoclonal antibody that inhibits receptor activator of nuclear factor-κB ligand (RANKL) and directly targets osteoclast-like giant cells. Compared to bisphosphonates like Alendronate, which reduce bone resorption indirectly, Denosumab acts more selectively and showed superior clinical response in this case. The therapeutic switch resulted in marked tumor regression, effectively reducing surgical burden and improving quality of life. This case highlights the complexity of managing OD-associated CGCGs and the potential of Denosumab as a more effective therapeutic option than Alendronate. However, ongoing surveillance for adverse effects such as medication-related osteonecrosis of the jaw, hypocalcemia, and atypical fractures is essential.
Ebata A, Tada H, Harada-Shoji N
… +4 more, Hamanaka Y, Miyashita M, Sato M, Ishida T
Tohoku J Exp Med
· 2026 Jun · PMID 41093592
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Since BRCA1/2 genetic testing has been covered by public insurance for certain breast cancer patients in Japan from April 2020, it has led to a rapid increase in testing frequency and the identification of many BRCA1/2 p...Since BRCA1/2 genetic testing has been covered by public insurance for certain breast cancer patients in Japan from April 2020, it has led to a rapid increase in testing frequency and the identification of many BRCA1/2 pathogenic variant (PV) carriers. BRCA1/2 PV carriers in Japan are reported to be also more susceptible to esophageal, gastric, and biliary tract cancers. We retrospectively analyzed 337 breast cancer patients who underwent BRCA1/2 genetic testing at Tohoku University Hospital. We extracted medical records such as past medical histories and family histories of malignancies based on questionnaires written by patient himself at first visit to our department. Compared to non-PV patients (n = 304), BRCA1/2 PV patients (n = 33) tended to have higher family history of breast cancer (P = 0.098) and had significantly higher family history of ovarian cancer (P = 0.005). However, pancreas, prostate, esophagus, gastric, and biliary tract cancer are not significantly more prevalent with family history among BRCA1/2 PV patients than non-PV patients. In following up on breast cancer patients with BRCA1/2 PV, at present, although there is a suggestion that esophageal, gastric, and biliary tract cancers are more likely to occur, the number of cases remains small, and it is not easy to perform these cancer screening on all BRCA1/2 PV cases.
Suzuki N, Takahashi T, Furusawa Y
… +18 more, Miyagi M, Miura T, Takanaka S, Yokouchi S, Ataka K, Soma S, Sekiguchi Y, Oya H, Ikeda K, Izumi R, Nakamura N, Shijo T, Kato M, Nakazawa C, Obata K, Okazaki T, Aoki M, Ebihara S
Tohoku J Exp Med
· 2026 Jun · PMID 41093591
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GNE myopathy is a progressive distal myopathy typically manifested in late teens to thirties. We described the effects of bilateral upper-extremity hybrid assistive limb (HAL) rehabilitation in patients with GNE myopathy...GNE myopathy is a progressive distal myopathy typically manifested in late teens to thirties. We described the effects of bilateral upper-extremity hybrid assistive limb (HAL) rehabilitation in patients with GNE myopathy. We described a case series of 6 patients with GNE myopathy. Among them, five patients underwent bilateral upper-extremity HAL rehabilitation during a two-week hospitalization. The rehabilitation program consisted of elbow flexion-extension exercises with gradually adjusted assistance levels. HAL rehabilitation resulted in significant improvements in upper extremity strength scores (p = 0.0311), and overall upper limb motor function assessed by Simple Test for Evaluating Hand Function (STEF) (p = 0.0014). No significant changes in creatine kinase (CK) levels were observed, indicating that the exercise intensity did not cause muscle damage. Patients reported functional improvements including increased ability to perform daily activities requiring elbow flexion. While further research is needed to assess long-term effects and underlying mechanisms, these findings suggest potential benefits of HAL rehabilitation for neuromuscular diseases with upper-limb weakness.
Lin X, Lin H, Luo M
… +4 more, Han J, Hu Y, Xiang S, Fang Z
Tohoku J Exp Med
· 2026 Jun · PMID 41062301
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ACTR2 is highly expressed and autophagy is enhanced in adriamycin (ADR)-induced nephropathic rats, and KLF5/ITCH is predicted to be the upstream axis of ACTR2. Accordingly, we investigated the regulation of excessive aut...ACTR2 is highly expressed and autophagy is enhanced in adriamycin (ADR)-induced nephropathic rats, and KLF5/ITCH is predicted to be the upstream axis of ACTR2. Accordingly, we investigated the regulation of excessive autophagy by KLF5/ITCH/ACTR2 in inflammatory ADR-induced podocytes and preliminarily elucidated the molecular mechanisms of podocyte injury. ADR was used to establish a rat model of nephropathy as well as a model of podocyte injury. Proteinuria, albumin, serum creatinine, triglyceride and total cholesterol levels, histopathologic staining were assessed. Transmission electron microscopy was applied to observe autophagy, and Western blot to detect protein levels in vivo. Quantitative real-time polymerase chain reaction, Western blot, flow cytometry, Co-immunoprecipitation, chromatin immunoprecipitation, dual luciferase reporter genes, and bioinformatics analyses were performed for in vitro mechanistic and functional exploration. ADR rat model was successfully constructed, showing abnormal biochemical indices, pathological damage and autophagic vesicle formation, increased expressions of autophagy-related proteins and ACTR2 protein, and decreased expressions of KLF5 and ITCH. ACTR2 silencing reversed ADR-induced apoptosis and inflammatory factors, decreased LC3 II/LC3 I, Beclin 1, Bax and C-caspase 3 levels, and elevated Bcl-2 level in vitro. KLF5 activated ITCH to promote ubiquitinated degradation of ACTR2. KLF5 overexpression attenuated the promoting effects of ACTR2 on autophagy, apoptosis, and inflammation in podocytes. The mechanism by which KLF5 protects podocytes from injury is to promote ITCH transcription, thereby facilitating ITCH-mediated ubiquitination of ACTR2 and inhibiting podocyte autophagy.
Tateda Y, Suzuki T, Sato T
… +4 more, Ise K, Shimada H, Nakamura Y, Ohta N
Tohoku J Exp Med
· 2025 Dec · PMID 41034039
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The vasohibin (VASH) family includes vasohibin-1 (VASH1), whose expression is induced in vascular endothelial cells by angiogenic factors such as vascular endothelial growth factor (VEGF) to inhibit angiogenesis via a ne...The vasohibin (VASH) family includes vasohibin-1 (VASH1), whose expression is induced in vascular endothelial cells by angiogenic factors such as vascular endothelial growth factor (VEGF) to inhibit angiogenesis via a negative feedback loop, and vasohibin-2 (VASH2), a homolog of VASH1, which is mainly expressed in cancer cells and promotes angiogenesis. VASH proteins have been associated with tumor angiogenesis and prognosis in various tumor types, drawing attention to their role in tumor biology. To date, no studies have reported on the expression of VEGF, VASH1, and VASH2 in benign parotid gland tumors. Therefore, in this study, we aimed to investigate their expression in these tumors. Our cohort consisted of 28 patients with benign parotid gland tumors, aged 31-79 (mean age 60.0 ± 13.8) years. Tumors were histologically diagnosed as pleomorphic adenoma in 15 patients (15 specimens) and Warthin's tumor in 13 patients (15 specimens). Immunohistochemical staining for VEGF, VASH1, and VASH2 was assessed based on the percentage of stained cells per slide (0-100%) and staining intensity, quantified using the H score. Immunostaining for VEGF, VASH1, and VASH2 demonstrated that Warthin's tumors had statistically significantly higher H-scores compared to pleomorphic adenomas. In addition, CD31 immunostaining showed a higher mean microvessel density (MVD) in the tumor stroma in Warthin's tumors than in pleomorphic adenomas. Similarly, CD34 immunostaining showed a higher number of spindle cells in the tumor stroma in Warthin's tumors than in pleomorphic adenomas. These findings suggest that the overexpression of VEGF, VASH1, and VASH2 is likely involved in the pathogenesis of benign parotid gland tumors.
Wu Z, Jin H, Shuai B
… +4 more, Zhang X, Wang S, Chen L, Huang B
Tohoku J Exp Med
· 2025 Dec · PMID 41034038
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The infection of Enterovirus A 71 (EV-A71) could cause severe hand-foot-mouth disease (HFMD), which poses a grave threat to the lives of children. Moreover, in some cases where the children survive, long-term neurologica...The infection of Enterovirus A 71 (EV-A71) could cause severe hand-foot-mouth disease (HFMD), which poses a grave threat to the lives of children. Moreover, in some cases where the children survive, long-term neurological sequelae may occur. Acupuncture can alleviate cognitive dysfunction, however, its role in HFMD has not been investigated. HFMD models caused by EV-A71 infection were constructed in Sprague-Dawley infant rats. Morris water maze test was employed to evaluate the spatial learning and memory function administered by the interventions of SB203580 or/and acupuncture. The changes and apoptosis degree of hippocampus tissues were evaluated by H&E staining and TUNEL staining assay. Furthermore, the western blot was utilized to detect the apoptosis-related proteins as well as the proteins involved in the p38 MAPK/CREB pathway within the tissues. After induction with EV-A71, compared to the control group, the rat's escape latency increased noticeably, while the time duration spent on the platform and the number of platform-crossing times decreased significantly. Moreover, in the hippocampal tissues of EV-A71-infected rats, pyramidal cells were loosely arranged, of different sizes and disorganized, as well as the numbers of neuronal cell apoptosis were significantly elevated, accompanied by increased expression of phosphorylated p38 and down-regulations of phosphorylated CREB. Interestingly, these effects could be relieved by acupuncture or SB203580 intervention, and acupuncture could additionally enhance the influence of SB203580. Acupuncture might modulate the p38 MAPK/CREB pathway, thus alleviating the impairment of spatial learning and memory, as well as the apoptosis in the hippocampus of rats infected with EV-A71.
The growth of cancer is one of the many physiological and pathological processes that depend on ferroptosis, an iron-dependent kind of programmed cell death. This study examines how ferroptosis is regulated and how it af...The growth of cancer is one of the many physiological and pathological processes that depend on ferroptosis, an iron-dependent kind of programmed cell death. This study examines how ferroptosis is regulated and how it affects the development of hepatocellular carcinoma (HCC) by means of the sterol carrier protein 2 (SCP2)/heat shock protein family B (small) member 1 (HSPB1) axis. The effects of SCP2 overexpression and the ferroptosis inhibitor Ferrostain-1 on cell viability, proliferation, invasion, and the expression of HSPB1 and ferroptosis-related genes were evaluated using cell counting kit-8 (CCK-8) assays, 5-Ethynyl-2'-deoxyuridine (EdU) assays, transwell assays, and protein expression analysis. Overexpression experiments were conducted to further explore the impact of HSPB1 and SCP2 on HCC cell function and ferroptosis. In HCC cell lines, overexpression of SCP2 significantly inhibited cell proliferation and invasion, while enhancing ferroptosis through the upregulation of malondialdehyde (MDA) and downregulation of antioxidant proteins glutathione peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) and nuclear factor erythroid 2-related factor 2 (Nrf2). Notably, overexpression of HSPB1 reversed the effects of SCP2 on the viability, proliferation, invasion and ferroptosis of HCC cells. The SCP2/HSPB1 axis plays a crucial role in HCC progression, SCP2 inhibits the progression of HCC by suppressing the expression of HSPB1 to inducing ferroptosis. According to our results, focusing on the SCP2/HSPB1 axis may offer a unique treatment strategy to manage the evolution of HCC and get past ferroptosis-related resistance.
Tohoku J Exp Med
· 2026 May · PMID 40993091
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This study aimed to assess the effectiveness of information-motivation-behavioral (IMB) skills model of hypothyroidism self-management integrated into prenatal care in pregnant women with newly diagnosed hypothyroidism....This study aimed to assess the effectiveness of information-motivation-behavioral (IMB) skills model of hypothyroidism self-management integrated into prenatal care in pregnant women with newly diagnosed hypothyroidism. Participants were 119 women with newly diagnosed hypothyroidism during pregnancy, with 59 cases receiving prenatal care based on IMB skills model (IMB group) and 60 cases receiving prenatal usual care (PUC group). The IMB group had a higher proportion of high adherence than the PUC group (48 vs. 35, P = 0.022). The incidence rate of preterm birth was significantly different between the IMB group and the PUC group (0 vs. 5, P = 0.024). The IMB group had fewer proportions of pregnant women with maternal stress, anxiety and depression than the PUC group (P < 0.05). Increased GSES scores indicating better self-efficacy and high patient satisfaction to prenatal care were noted in the IMB group compared to the PUC group post intervention (P < 0.05). The study demonstrates IMB skills model of hypothyroidism self-management integrated into prenatal care is found to be effective in improving medication adherence, mental health and self-efficacy in pregnant women with newly diagnosed hypothyroidism.
Yusufu A, Du R, Mardan M
… +5 more, Xie X, Cai X, Xu T, Sheng W, Mamat M
Tohoku J Exp Med
· 2026 May · PMID 40993090
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Spinal tuberculosis (STB) is more common in children and young adults and can lead to neurological deficits and spinal deformity in severe cases. Therefore, it is important to find a biomarker for studying the pathogenes...Spinal tuberculosis (STB) is more common in children and young adults and can lead to neurological deficits and spinal deformity in severe cases. Therefore, it is important to find a biomarker for studying the pathogenesis of STB. RT-qPCR was utilized to measure the expression of miR-199b-5p in STB. Moreover, the GPL33147 database was utilized to validate the expression of miR-199b-5p in both STB patients and healthy individuals. Pearson's correlation analysis was employed to explore the correlation between miR-199a-5p and Glucosaminyl (N-acetyl) transferase 2 (Gcnt2) in peripheral blood mononuclear cells (PBMCs) and tissues from STB. The Mycobacterium tuberculosis (Mtb) infection model was constructed by infecting THP-1 cells with 10 multiplicity of infection (MOI) Mtb for 24 h. Overexpressed and inhibited miR-199b-5p and Gcnt2 in the Mtb infection model were realized by cell transfection. ELISA was utilized to measure the expression of inflammatory factors. The target of miR-199b-5p was forecasted by TargetScan. Additionally, the interaction between miR-199b-5p and Gcnt2 was elucidated through the implementation of a dual-luciferase assay system. The expression of miR-199b-5p was increased in PBMCs and tissues from STB, and the two were positively correlated. Gcnt2 was the downstream target of miR-199b-5p, and they negatively correlated in PBMCs and tissues from STB. In the Mtb infection model, overexpressed miR-199b-5p increased inflammatory factors, and overexpression of Gcnt2 reduced this increase. miR-199b-5p was increased in STB, which was a potential biomarker of STB. Notably, miR-199b-5p and Gcnt2 exhibited a collaborative role in STB, displaying an inverse correlation with one another within this context.
Tohoku J Exp Med
· 2026 May · PMID 40993089
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MicroRNA (miRNA), as a molecule with regulatory functions, has become a research hotspot for diagnosis or prognosis in various diseases. This study investigates the predictive value of miR-205-5p concerning abdominal adh...MicroRNA (miRNA), as a molecule with regulatory functions, has become a research hotspot for diagnosis or prognosis in various diseases. This study investigates the predictive value of miR-205-5p concerning abdominal adhesions and postpartum complications in women undergoing secondary cesarean sections with a scarred uterus. Before the second cesarean section, the condition of the scar was evaluated using the Patient and Observer Scar Assessment Scale (POSAS). The serum levels of miR-205-5p were quantified through RT-qPCR, and the pregnant women were divided into the high-expression group and the low-expression group based on the average level of miR-205-5p. Subsequent analyses compared the abdominal scar scores, pelvic floor muscle fiber strength, incidence of abdominal adhesions, and the rate of postpartum complications. The predictive value of miR-205-5p for abdominal adhesions and complications was assessed via receiver operating characteristic (ROC) curve. The findings revealed a negative correlation between miR-205-5p levels and POSAS scores. Furthermore, the incidence of abdominal adhesions and postpartum complications was significantly lower in the high-expression group compared to their low-expression counterparts. The area under the curve (AUC) for predicting abdominal adhesions and postpartum complications using miR-205-5p was determined to be 0.822 and 0.831, respectively. Notably, no significant differences were observed in pelvic floor muscle function between the two groups. In conclusion, this study posits that miR-205-5p holds substantial promise as a predictive biomarker for abdominal adhesions and postpartum complications in women with a scarred uterus.