Colloids Surf B Biointerfaces [JOURNAL]
Sun
200 papers
NIR-activated nanocomplexes based on novel PEG-Chlorin p6 derivatives for photodynamic therapy.
Nikolaeva ME, Demina PA, Khaydukov KV … +6 more , Trifanova EM, Khochenkov DA, Maslov MA, Generalova AN, Khaydukov EV, Akasov RA
Colloids Surf B Biointerfaces · 2026 May · PMID 42142473 · Publisher ↗
Photodynamic therapy (PDT) is a rapidly evolving approach based on the elimination of target cells with light-activated photosensitizers (PS). The main limitation of PDT is the low penetration of visible light used to ac...
Photodynamic therapy (PDT) is a rapidly evolving approach based on the elimination of target cells with light-activated photosensitizers (PS). The main limitation of PDT is the low penetration of visible light used to activate PS. Upconversion nanoparticles (UCNPs) provide a unique platform for the development of systems based on deep-penetrating near-infrared (NIR) light due to their ability to absorb NIR light and emit visible light capable of activating PS. However, UCNPs are usually hydrophobic and therefore require surface hydrophilization and biofunctionalization. In this work, we have developed a one-step technique to form a NIR-activated PDT nanocomplex consisting of NaYF:YbEr/NaYF particles functionalized with novel amphiphilic chlorin derivatives by hydrophobic self-assembly to address both light penetration and UCNP surface functionalization issues. To this end, three novel chlorophyll a derivatives based on chlorin p with a hydrophobic octadecylamine moiety and a hydrophilic PEG and PEG moieties were synthesized. Förster resonance energy transfer was demonstrated for these nanocomplexes, and one of them, namely the complex with compound 4 (octadecyl-Chlp-PEG) with 19% FRET efficiency, was selected for further investigation. The in vitro cytotoxicity of compound 4 was studied using murine fibroblast L-929, human fibroblast WI-26 and human breast adenocarcinoma MCF-7 cell lines, and a 3-fold higher phototoxicity index was found in comparison with the commercial Photoditazine (Chlorin е). The in vivo study using the Lewis Lung Cancer (LLC) model demonstrated 41.2% tumor growth inhibition efficacy compared to the control group on day 14 after irradiation. We conclude that NIR-activated nanocomplexes of UCNPs-compound 4, as well as water-soluble novel amphiphilic chlorophyll a derivatives, can be promising for PDT purposes. The obtained nanocomplex can be considered as the next generation of PSs which means the coupling of PSs with carriers for targeted delivery of antitumor drugs.
Structure-guided design of ionizable lipids with distinct amine headgroups for muscle-selective mRNA delivery and enhanced antitumor immunotherapy.
Lv H, Liu W, Zhang M … +3 more , Gao X, Liu F, Yang C
Colloids Surf B Biointerfaces · 2026 May · PMID 42142472 · Publisher ↗
The development of messenger RNA (mRNA)-based cancer immunotherapies has gained significant attention due to their potential to activate robust immune responses against tumors. However, a major challenge remains in devel...
The development of messenger RNA (mRNA)-based cancer immunotherapies has gained significant attention due to their potential to activate robust immune responses against tumors. However, a major challenge remains in developing an efficient delivery system that targets mRNA to specific tissues while minimizing off-target accumulation in the liver. Ionizable lipid nanoparticles (LNPs) are a promising platform for mRNA vaccine delivery, but achieving tissue-selective delivery is essential for maximizing both safety and efficacy. In this study, we synthesized a series of ionizable lipids with varying amine head groups and hydrophobic tail lengths using a Michael addition reaction and incorporated them into LNPs. Through systematic screening of physicochemical properties and in vitro mRNA expression profiles, spermine was identified as the optimal amine head group. Further optimization of hydrophobic tail chain length led to the identification of 114-LNP as the most effective formulation. Compared with SM-102-based LNP (SM102-LNP), 114-LNP offers distinct advantages for muscle-selective mRNA delivery, achieving efficient expression in muscle tissue while markedly reducing off-target accumulation in organs such as the liver, thereby minimizing the risk of off-target toxicity. When loaded with ovalbumin (OVA) mRNA, 114-LNP enhanced antigen presentation and dendritic cell maturation, leading to robust anti-tumor immune responses and significant inhibition of tumor growth in E.G7-OVA tumor-bearing mice. Overall, this study demonstrates that rational molecular design and systematic screening of ionizable lipids can yield LNPs with improved tissue-selective mRNA delivery and therapeutic performance, highlighting 114-LNP as a safe and effective platform for mRNA-based cancer immunotherapy.
3D Flower-Like BiOCO/2D rGO composite modified with GCE for electrochemical nanomolar quantification of chloramphenicol in biological and environmental matrices.
Zhu F, Xu K, Ling H … +4 more , Chao J, Li J, Liu J, Yang C
Colloids Surf B Biointerfaces · 2026 May · PMID 42142471 · Publisher ↗
Chloramphenicol (CAP) remains prevalent in aquaculture despite its classification as a Group 2 A carcinogen. Given the associated health risks, establishing robust detection methods for CAP is imperative. Herein, we inno...
Chloramphenicol (CAP) remains prevalent in aquaculture despite its classification as a Group 2 A carcinogen. Given the associated health risks, establishing robust detection methods for CAP is imperative. Herein, we innovatively propose the three-dimensional (3D) flower-like BiOCO/rGO composites for CAP electrochemical sensing. The best sensing parameters (pH, scan rate, accumulation conditions) were systematically investigated. As a result, the BiOCO/rGO/GCE sensor exhibits a peak current density of 381.91 µA/cm, ultra-low detection limit (0.362 nM), and two wide linear ranges (0.001-10 μM, 10-100 μM), enabling both trace and high-concentration quantitative detection of CAP. These high sensing performances could be ascribed to the synergistic effect between rGO and BiOCO, which improves the stability of BiOCO/rGO composites. Their interfacial interaction accelerates electron transfer, lowers activation energy, and forms an efficient 3D electron/mass transport network. This sensor also applied in detecting CAP in lake water and milk samples with satisfied recovery rates. These findings underscore the BiOCO/rGO/GCE as a promising platform for sensitive CAP monitoring in environmental and food safety applications.
De Novo developing nanoplatform encapsuling α-arbutin and α-syn inhibitor for precise treatment of Parkinson's disease.
Yang Y, Ma B, Yuan Y … +8 more , Wang N, Gao Q, Li C, Li X, Wu H, Zhao Y, Guo L, Zhang C
Colloids Surf B Biointerfaces · 2026 May · PMID 42142470 · Publisher ↗
Parkinson's disease (PD), the second most common neurodegenerative disorder, is characterized by progressive degeneration of dopaminergic neurons in the midbrain. Currently, PD remains incurable, though administration of...
Parkinson's disease (PD), the second most common neurodegenerative disorder, is characterized by progressive degeneration of dopaminergic neurons in the midbrain. Currently, PD remains incurable, though administration of levodopa or deep brain stimulation could partially ameliorate symptoms of PD. Mechanistically, PD is underlined by mitochondrial dysfunction, oxidative stress, α-synuclein (α-Syn) aggregation and neuroinflammation. Hence, intervention targeting those pathogenetic factors hold great potential for effective treatment of PD. In present study, we developed one nanoplatform encapsuling natural antioxidant (α-arbutin) and α-synuclein inhibitor (Anle138b) for precise treatment of PD by blood brain barrier (BBB) penetrating peptide facilitated intra brain delivery. The endowed property of as developed nanoplatform, HMPDA@AA/RVG, was were characterized. Both in vitro and in vivo experiments confirmed that HMPDA@AA/RVG exerted neuroprotective effects by ameliorating oxidative stress, mitochondrial dysfunction and α-Syn aggregation. HMPDA@AA/RVG was specifically distributed in the brain, successfully rescued dopaminergic neuron loss and the behavioral deficits of PD mice. In summary, by targeting multiple pathological features of PD, we developed one multiple functional nanoplatform for that effectively mitigated PD phenotypes. Our study paves the way for precise intervention of neurodegenerative diseases by leveraging the nanomaterials facilitated delivery of natural medicine and chemical compounds.
Preparation and mechanism of heterogeneous MX@MIL-125(Ti) towards sonodynamic therapy.
Li J, Wang X, Yang F … +4 more , Diao H, Wang J, Wang Z, Li Z
Colloids Surf B Biointerfaces · 2026 May · PMID 42140135 · Publisher ↗
Sonodynamic therapy (SDT), as a burgeoning non-invasive treatment technology, can utilize ultrasound (US) irradiation to excite sonosensitizers for generating reactive oxygen species (ROS), thereby achieving to kill deep...
Sonodynamic therapy (SDT), as a burgeoning non-invasive treatment technology, can utilize ultrasound (US) irradiation to excite sonosensitizers for generating reactive oxygen species (ROS), thereby achieving to kill deep-seated tumors. However, the development of sonosensitizers with high sonosensitivity and good biocompatibility remains a large challenge. Herein, a novel titanium-based metal-organic frameworks (Ti-MOFs) was successfully fabricated by introducing TiC MXene to replace the traditional titanium (Ti) source of MIL-125(Ti), and served as a heterogeneous sonosensitizer to construct high-efficiency SDT platform. Through structural design and surface modification, the obtained MX@MIL-125(Ti) was optimized to have a size of approximately 112 nm, and its sonosensitive performance and biocompatibility was also enhanced significantly. Both in vitro and in vivo experiments confirmed that such MX@MIL-125(Ti) exhibited superior ROS generation capability under US irradiation compared to traditional MIL-125(Ti), and could achieve high-efficiency anti-tumor activity, with a tumor inhibition rate exceeding 80%. In addition, the excellent biocompatibility of MX@MIL-125(Ti) provided significant support for clinical translation. In a word, this study seeks some breakthroughs for the development of high-sensitivity good-biocompatibility sonosensitizers, and will promote the practical application of SDT in tumor treatment.
An HSP90-inhibitory thermosensitive hydrogel enhances photothermal therapy against triple-negative breast cancer.
Wang K, Xu T, Li R … +7 more , Tao C, Chen W, Mao S, Zhou M, Liao B, Shang J, Deng L
Colloids Surf B Biointerfaces · 2026 May · PMID 42140134 · Publisher ↗
Triple-negative breast cancer (TNBC) is highly aggressive and prone to recurrence, and durable therapeutic efficacy is often difficult to achieve with monotherapy. Photothermal therapy (PTT) offers advantages such as min...
Triple-negative breast cancer (TNBC) is highly aggressive and prone to recurrence, and durable therapeutic efficacy is often difficult to achieve with monotherapy. Photothermal therapy (PTT) offers advantages such as minimal invasiveness and spatiotemporal controllability and induces tumor cell death through localized hyperthermia. However, thermal stimulation simultaneously activates protective heat-stress responses in tumor cells, among which the inducible upregulation of heat shock protein 90 (HSP90) enhances thermotolerance and consequently compromises the efficacy of PTT. To overcome this limitation, we proposed a synergistic strategy based on HSP90 inhibition to induce tumor cell apoptosis while sensitizing tumors to PTT, and constructed an intratumorally injectable thermosensitive hydrogel for localized drug delivery. The HSP90 inhibitor geldanamycin (GA) was loaded into mesoporous polydopamine (MPDA) to form MGA, which was subsequently encapsulated within a PNIPAM/β-glucan composite thermosensitive hydrogel, yielding MGA@Gel. Both in vitro and in vivo studies demonstrated that MGA@Gel markedly enhanced antitumor efficacy under near-infrared (NIR) laser irradiation and effectively suppressed HSP90-associated heat-stress tolerance. Notably, in a 4T1 tumor-bearing mouse model, a single intratumoral injection established a sustained local drug depot. When combined with NIR laser irradiation, this system enabled efficient tumor suppression, pronounced downregulation of HSP90 signaling, increased apoptosis, and reduced tumor cell proliferation. These findings confirm that GA, by blocking the heat-stress protective pathway, effectively potentiates the antitumor effects of PTT. Overall, we developed a thermosensitive in situ hydrogel delivery system that enhances PTT via HSP90 inhibition, thereby overcoming tumor thermoresistance and providing a promising strategy for precise local treatment of TNBC.
Bio-enhanced NGV/QC-Zn@ZSM-5 zeolite nanoplatform for stabilizing the dentin-adhesive interface.
Qiao G, Li H, Liu X … +9 more , Zhang H, Song J, Alshawwa H, Zou X, Han R, Tang W, Shangguan R, Yan W, Zhang Z
Colloids Surf B Biointerfaces · 2026 May · PMID 42140133 · Publisher ↗
Dentin adhesives are essential for minimally invasive restorative dentistry. However, the long-term stability of the resin-dentin interface remains limited due to collagen degradation and bacterial erosion. Achieving syn...
Dentin adhesives are essential for minimally invasive restorative dentistry. However, the long-term stability of the resin-dentin interface remains limited due to collagen degradation and bacterial erosion. Achieving synergistic antibacterial activity, collagen cross-linking, and remineralization at this interface is therefore crucial for durable dentin bonding. Quercetin (QC), a natural polyphenol, possesses both antibacterial activity and collagen cross-linking capability. These properties can be further enhanced through coordination with metal ions to form metal-phenolic networks (MPNs). In this study, we used the NGV peptide, derived from bone sialoprotein (BSP, a non-collagenous protein), as a remineralization motif. We combined NGV with an MPN to construct a bio-enhanced nanoplatform (NGV/QC-Zn@ZSM-5) for dentin adhesives. The nanoplatform was prepared by incorporating Zn into ZSM-5 zeolites, followed by surface coating with a QC-Zn MPN and subsequent functionalization with NGV. ZSM-5 serves as a multifunctional carrier due to its high ion-exchange capacity, tunable surface chemistry, and ability to enable controlled Zn release. The resulting system is designed to stabilize collagen, inhibit bacterial biofilm formation, and promote dentin remineralization. This integrated strategy strengthens the resin-dentin interface against both enzymatic and bacterial degradation, thereby improving bonding durability. Notably, after aging, the modified adhesive exhibited a bond strength approximately 2.03-fold higher than that of the commercial control. Overall, these results highlight the strong potential of this nanoplatform to enhance the longevity and clinical performance of dental bonding restorations.
Antigen-specific IgY as an orally deliverable biomaterial for sustained immunomodulation in ulcerative colitis.
Ma B, Bai G, Li H … +9 more , Wang Y, Yin X, Jiang Y, Ning Y, Wang H, Liu P, Tao M, Liu D, Wang H
Colloids Surf B Biointerfaces · 2026 May · PMID 42140132 · Publisher ↗
Over the past decades, antibodies have become indispensable tools due to their high specificity and versatility. Egg yolk antibody (IgY), a natural protein biomaterial noted for its biosafety, thermal stability, and cost...
Over the past decades, antibodies have become indispensable tools due to their high specificity and versatility. Egg yolk antibody (IgY), a natural protein biomaterial noted for its biosafety, thermal stability, and cost-effective, is particularly suitable for long-term interventions in chronic diseases. Its intrinsic biocompatibility allows safe oral administration, while its structural robustness facilitates diverse delivery formats such as bioactive formulations or coatings. This makes IgY a promising candidate for managing conditions like inflammatory bowel disease (IBD), including ulcerative colitis (UC), which require prolonged, well-tolerated therapeutic strategies. However, the antigen-specific therapeutic potential of IgY in IBD remains underexplored. Here, we propose to use an engineered anti-TNFR1 IgY as an oral therapeutic agent for UC intervention as a TNFR1-selective strategy. Anti-TNFR1 IgY exhibited superior gastrointestinal stability to mammalian IgG and suppressed the production of key inflammatory mediators, such as IL-6 and MMP9, in stimulated RAW264.7 macrophages. In a DSS-induced murine colitis model, oral administration of anti-TNFR1 IgY significantly alleviated inflammation, evidenced by reduced biomarkers (calprotectin, IL-6, TNF-α) and elevated IL-10, alongside improved behavioral manifestations and attenuated histopathological staining. Moreover, it retained bioactivity following heating, freeze-drying, and hydrogel encapsulation, supporting its potential as a scalable robust bioactive building block. Our work highlights antigen-specific IgY as a biocompatible biomaterial-based strategy for long-term immunomodulation in intestinal inflammation. This platform may help connect orally administered targeted therapy with gastrointestinal health management.
Eco-friendly clinoptilolite/NH-chitosan/PVA nanofibers for gentamicin delivery, biofilm disruption, and burn wound healing.
Zendehdel M, Alizadeh N, Komijani M … +1 more , Soltaninejad H
Colloids Surf B Biointerfaces · 2026 May · PMID 42140131 · Publisher ↗
Biofilm-associated infections and delayed wound healing remain major challenges in burn treatment due to limited antibiotic penetration and reduced therapeutic efficacy. In this study, we developed an eco-friendly gentam...
Biofilm-associated infections and delayed wound healing remain major challenges in burn treatment due to limited antibiotic penetration and reduced therapeutic efficacy. In this study, we developed an eco-friendly gentamicin delivery system based on 3-aminopropyltriethoxysilane (APTES)-functionalized clinoptilolite incorporated into chitosan/poly (vinyl alcohol) (PVA) electrospun nanofibers. The nanofibers were fabricated from a homogeneous precursor solution to ensure uniform distribution of all components. Comprehensive physicochemical characterization (FT-IR, XRD, FESEM, TGA, and zeta potential) confirmed successful surface functionalization, high drug-loading efficiency, and uniform fiber morphology. Drug release studies demonstrated sustained and pH-responsive behavior, with gentamicin release exceeding 80% after 8 h for the nanofiber system and approximately 5 h for the Clin/NH₂/Gen powder formulation. Antibacterial and antibiofilm assays revealed rapid biofilm disruption within 30 min and a significant reduction in Staphylococcus aureus viability over 24 h. In vivo evaluation using a third-degree burn model showed that the nanofiber-treated group achieved near-complete re-epithelialization, enhanced angiogenesis, and well-organized collagen deposition, outperforming all control groups. These findings demonstrate that amino-functionalized clinoptilolite-based nanofibers provide an effective and sustainable platform for controlled antibiotic delivery, with strong potential for the treatment of biofilm-associated burn wound infections.
Electrostatically assembled CaO@MPN-HA nanoreactors potentiate anti-PD-1 therapy in thyroid cancer via synergistic ferroptosis and calcium overload.
Hu Y, Liu L, Li Z … +3 more , Liu J, Yang C, Shao L
Colloids Surf B Biointerfaces · 2026 May · PMID 42140130 · Publisher ↗
Immune checkpoint blockade (ICB) therapy has achieved remarkable breakthroughs in clinical cancer treatment; however, its efficacy is often limited by the immunosuppressive characteristics of the tumor microenvironment (...
Immune checkpoint blockade (ICB) therapy has achieved remarkable breakthroughs in clinical cancer treatment; however, its efficacy is often limited by the immunosuppressive characteristics of the tumor microenvironment (TME) and the insufficient infiltration of cytotoxic T lymphocytes. Inducing immunogenic cell death (ICD) to convert "cold tumors" into "hot tumors" is an effective strategy to overcome this barrier. Herein, we propose a new ion-interference immunotherapy strategy based on the synergistic action of "ferroptosis-calcium overload" and construct a multi-responsive nanoreactor (CaO₂@MPN-HA) with a layer-by-layer (LbL) self-assembled architecture. To address the intrinsic electrostatic repulsion between the negatively charged metal-polyphenol network (MPN) and the targeting ligand hyaluronic acid (HA), we innovatively introduce the cationic polymer polyethyleneimine (PEI) as an "electrostatic bridge," enabling surface charge reversal through electrostatic attraction. This strategy successfully constructs a robust core-shell structure and endows the material with lysosomal escape capability. Upon entering tumor cells, the nano-reactor undergoes responsive disassembly in the acidic and glutathione (GSH)-rich TME, releasing tannic acid to facilitate the reduction of Fe³ ⁺ to highly active Fe²⁺ and catalyze the efficient Fenton reaction fueled by self-supplied H₂O₂ from the CaO₂ core. Meanwhile, the burst release of Ca²⁺ induces mitochondrial dysfunction and synergistically amplifies oxidative stress. This cascade assault potently triggers ferroptosis-dominated ICD, promoting dendritic cell maturation and effector T-cell infiltration. In a TtT/GF thyroid tumor-bearing mouse model, the combination of CaO2@MPN-HA with anti-PD-1 therapy significantly suppressed primary tumor growth and promoted systemic antitumor immune activation and immune memory. This work not only provides an efficient "in situ vaccine" strategy for refractory thyroid cancer but also offers a generalizable materials-science solution to overcome electrostatic repulsion in the interfacial assembly of nanomedicines.
Eliciting ferroptosis with a visual magnetic nanogel to sensitize triple negative breast cancer to taxol by blocking extracellular vesicle-mediated iron efflux.
Wu Y, Xu D, Xu J … +3 more , Dong L, Chen X, Zhang W
Colloids Surf B Biointerfaces · 2026 May · PMID 42134156 · Publisher ↗
Although ferroptosis demonstrates significant potential in combating drug-resistant cancers, the efficacy of iron-based nanomaterials is often limited by insufficient iron accumulation within tumors, a problem further ex...
Although ferroptosis demonstrates significant potential in combating drug-resistant cancers, the efficacy of iron-based nanomaterials is often limited by insufficient iron accumulation within tumors, a problem further exacerbated by extracellular vesicle (EV)-mediated iron efflux. To address this challenge, we developed an acid-responsive magnetic nanogel (MNG) via Schiff base crosslinking for the co-delivery of Prominin2 small interfering RNA (siProminin2) and ultrasmall iron oxide nanozymes. In paclitaxel-resistant triple-negative breast cancer (MDA-MB-231/TaxR) cells, siProminin2@MNG promotes ferroptosis through dual mechanisms: direct delivery of iron oxide nanozymes that exhibit peroxidase-like activity to catalyze the Fenton reaction, thereby intensifying reactive oxygen species (ROS) production, and simultaneous suppression of EV-mediated iron export via Prominin2 knockdown. Ferroptosis induction was confirmed by deferoxamine (DFO) rescue assays, along with elevated levels of ROS, lipid peroxidation (LPO), malondialdehyde (MDA), and decreased GPX4 protein expression. Furthermore, nanoparticle tracking analysis (NTA) and downregulated TSG101 expression validated the inhibition of extracellular vesicle secretion following Prominin2 knockdown. The degradation of MNG within acidic lysosomes not only facilitates cargo release but also enhances T1-weighted MRI contrast due to changes in the aggregation state of the nanozymes, enabling non-invasive monitoring of siRNA release. In a tumor-bearing mouse model, siProminin2@MNG effectively sensitized MDA-MB-231/TaxR tumors to paclitaxel, leading to significant tumor growth inhibition. Mechanistic studies confirmed that this synergistic therapeutic outcome was mediated through the ferroptosis pathway. This rationally designed platform provides a powerful theranostic strategy for synergistic ferroptosis therapy.
Supramolecular self-assembly mechanisms, interfacial behavior, multimodal antimicrobial and anti-adhesion activities of the novel Tilapia piscidin-3 (TP3) peptide hydrogel.
Selvaraj SP, Yeh JC, Chang JC … +8 more , Shanmugam R, Raja K, Chen QH, Chang CF, Lin WC, Huang JR, Li TL, Chen JY
Colloids Surf B Biointerfaces · 2026 May · PMID 42134155 · Publisher ↗
Supramolecular self-assembly of antimicrobial peptides (AMPs) from simple monomers to complex colloidal networks holds great potential for functional biomaterials. Self-assembled AMP (SA-AMP) hydrogels can serve dual pur...
Supramolecular self-assembly of antimicrobial peptides (AMPs) from simple monomers to complex colloidal networks holds great potential for functional biomaterials. Self-assembled AMP (SA-AMP) hydrogels can serve dual purposes by providing a physical scaffold and simultaneously eliminating drug-resistant pathogens. Despite the extensive medicinal potential of AMPs, only a few are known to self-assemble into hydrogels. Here, we first report a natural AMP, Tilapia piscidin-3 (TP3), that spontaneously self-assembles into a physically cross-linked hydrogel under physiological conditions without the need for chemical cross-linkers. Cryo-SEM, TEM, and high-resolution microscopy revealed that TP3 nanofibers exhibit twisted β-sheet architectures, forming hierarchical nanostructures with honeycomb-like microstructures. The self-assembly mechanisms were further elucidated by molecular dynamics simulations based on the NMR solution structure, revealing a hierarchical secondary-structure transition mediated by non-covalent interactions in the order: helix → coil/turn → β-bridge → β-sheet. Rheological studies demonstrated that TP3 hydrogel exhibits evident viscoelasticity, shear-thinning, and self-healing properties. Functionally, the hydrogel is biocompatible, non-hemolytic, and anti-inflammatory, demonstrating interfacial selectivity without intrinsic cytotoxicity. TP3 hydrogel exhibits broad-spectrum antimicrobial and antibiofilm activity against drug-resistant pathogens, including Pseudomonas aeruginosa and Candida albicans, via an interfacial driven "trap-and-kill" membranolytic mechanism. Proteomic analysis of the TP3 monomers revealed multimodal actions that disrupt bacterial metabolism, energy production, DNA repair, translation, and virulence pathways, driving catastrophic cellular collapse. The TP3 hydrogel reduced post-operative peritoneal adhesions in vivo. Overall, TP3 hydrogel represents a multifunctional soft nanobiomaterial, highlighting the potential of peptide-driven supramolecular self-assembly and interfacial design for antimicrobial and anti-adhesion applications.
Anisotropic magnetic particles with different dimensions, morphologies and surface grafting for magnetic field-assisted biofilm removal.
Zaveršek N, Caf M, Pautu V … +12 more , Marger L, Esmaeilnejad-Ahranjani P, Janež N, Zupan T, Marger F, Parga A, Haberl-Meglič S, Mekki M, Berlec A, Milošević I, Kralj S, Sabotič J
Colloids Surf B Biointerfaces · 2026 May · PMID 42134154 · Publisher ↗
Microorganisms in biofilms are protected from environmental stressors and therefore exhibit strong resistance to conventional removal strategies, including chemical disinfectants and antibiotics. In this study, we system...
Microorganisms in biofilms are protected from environmental stressors and therefore exhibit strong resistance to conventional removal strategies, including chemical disinfectants and antibiotics. In this study, we systematically evaluated nanomaterial-based removal methods on Listeria innocua biofilms. Anisotropic magnetic particles, composed of iron oxide, and silver nanoparticles, known for their intrinsic antibacterial properties, were used to assess the potential of nanostructure-triggered biofilm disruption. We investigated how particle surface roughness and size affect biofilm removal under magnetic actuation, using both classical colony-forming unit quantification (viability assessment) and fluorescence-based detection via a reporter protein. The surface roughness and size of anisotropic magnetic particles only modestly affected biofilm disruption. Conversely, a synergistic effect was observed when anisotropic magnetic particles were grafted with silver nanoparticles. Furthermore, we used Enterococcus faecalis and Candida albicans biofilms and observed pronounced species-dependent variability of the silver-based treatments. Our results indicate that hybrid magneto-chemical strategies represent a promising and likely necessary approach for reliable and robust biofilm removal.
GPTMS-chitosan-modified dendritic mesoporous silica nanoadjuvants synergistically enhance humoral and cellular immune responses.
Hu X, Tang E, Li F … +4 more , Wang R, Cao S, Wang C, Wu H
Colloids Surf B Biointerfaces · 2026 May · PMID 42127778 · Publisher ↗
Vaccine adjuvants are critical for enhancing immune responses. Although aluminum-based adjuvants are widely used in clinical practice and can improve immunogenicity, they exhibit significant limitations, including limite...
Vaccine adjuvants are critical for enhancing immune responses. Although aluminum-based adjuvants are widely used in clinical practice and can improve immunogenicity, they exhibit significant limitations, including limited immunostimulatory capacity and difficulty in precisely controlling response quality. They primarily induce humoral immunity, show limited ability to activate cellular immunity, and are associated with potential risks of cumulative neurotoxicity. In this study, dendritic mesoporous silica nanoparticles (MSN) with uniform particle size were synthesized and subsequently functionalized with amino groups, epoxy groups, or epoxy-chitosan to generate the adjuvant systems MSN-APTES, MSN-GPTMS, and MSN-GPTMS-CS, respectively. All adjuvant formulations exhibited well-controlled physicochemical properties and favorable biocompatibility. Using ovalbumin (OVA) as a model antigen, the adjuvant activities of MSN, MSN-APTES, MSN-GPTMS, and MSN-GPTMS-CS were evaluated in mice. MSN-GPTMS-CS induced a strong IgM response by day 14 and significantly increased IgG1, IgG2a, and IgG2b titers by day 28, with humoral immunity comparable to that induced by aluminum adjuvants. The elevated IgG2a levels suggest effective activation of cellular immunity, indicating concurrent humoral and cellular immune responses, and no obvious systemic toxicity was observed. Collectively, MSN-GPTMS-CS represents a promising strategy capable of simultaneously eliciting potent humoral and cellular immune responses, thereby providing a foundation for the development of engineered adjuvant-based vaccines that induce coordinated immune activation.
Tuning complexation and functionality of lactoferrin-low methoxyl pectin complexes by chlorogenic acid during acidification.
Fu W, He Y, Tian G … +4 more , Ma Z, Xie G, Zhang Y, Wang C
Colloids Surf B Biointerfaces · 2026 May · PMID 42127777 · Publisher ↗
The interactions between chlorogenic acid (CA) and lactoferrin (LF)-low methoxyl pectin (LMP) electrostatic complexes were examined during gradual acidification starting from an initial alkaline condition (pH 10). CA mod...
The interactions between chlorogenic acid (CA) and lactoferrin (LF)-low methoxyl pectin (LMP) electrostatic complexes were examined during gradual acidification starting from an initial alkaline condition (pH 10). CA modulated the physicochemical and functional properties of LF-LMP complexes, particularly at pH range of 5-8. CA reduced the hydrodynamic diameter of the LF-LMP complexes and decreased the intrinsic fluorescence intensity and surface hydrophobicity, whereas surface hydrophobicity increased with rising pH. No significant changes in the secondary structure were observed. LF-LMP-CA exhibited enhanced emulsifying activity, emulsifying stability, and foam stability, as well as improved DPPH and ABTS radical scavenging capacities compared to LF-LMP. In addition, none of the complexes showed apparent cytotoxic effects on the Caco-2 cells under the conditions tested. These resulted indicated that CA can modulate the complexation behavior, structure, and functionality of LF-LMP complexes. These findings provide a basis for the development of protein-polysaccharide-polyphenol complexes in food systems.
Biocorrosion behavior of high-purity Mg and Mg-Zn-Zr-Nd alloy during exposure to Porphyromonas gingivalis.
Alansy AS, Shi X, Yang Y … +2 more , Wang Q, Wang W
Colloids Surf B Biointerfaces · 2026 May · PMID 42127776 · Publisher ↗
Biodegradable magnesium (Mg) materials are attractive for guided bone regeneration, but oral exposure may trigger microbiologically influenced corrosion. This study compared the early corrosion behavior of high-purity Mg...
Biodegradable magnesium (Mg) materials are attractive for guided bone regeneration, but oral exposure may trigger microbiologically influenced corrosion. This study compared the early corrosion behavior of high-purity Mg (pMg) and Mg-2Zn-0.5Zr-0.5Nd (Mg-Nd) during anaerobic exposure to Porphyromonas gingivalis. Disk specimens were immersed in supplemented brain-heart infusion medium with or without P. gingivalis for 1, 3, and 7 days. Corrosion was evaluated by ICP-MS, pH monitoring, gravimetric mass loss, SEM/EDS, XRD, XPS, 3D optical profilometry, electrochemical impedance spectroscopy, and potentiodynamic polarization. Bacterial survival was assessed by CFU counting and Live/Dead imaging. Both materials rapidly alkalinized the medium and released Mg during immersion. P. gingivalis increased early mass-loss corrosion rates for both substrates and limited the development of a high-resistance interfacial state, with the strongest effect observed for Mg-2Zn-0.5Zr-0.5Nd. After 7 days, the alloy exhibited a substantially lower polarization resistance in the biotic condition than in the sterile control (700 versus 6250 Ωcm). Surface deposits in all groups were dominated by Mg- and O-containing species, but bacterial exposure altered deposit morphology and spatial distribution. Profilometry showed reduced maximum pit depth in the presence of P. gingivalis despite sustained electrochemical activity, consistent with redistribution rather than simple amplification of localized attack. Mg-2Zn-0.5Zr-0.5Nd reduced bacterial viability in suspension relative to pMg, but this antibacterial effect did not correspond to electrochemical stabilization. These findings indicate that P. gingivalis remodels the early corrosion interface of Mg-based biomaterials and should be considered in the preclinical evaluation of oral Mg devices.
Exosome-encapsulated CuPt-S hybrid nanoclusters: Triple enzyme activities for cuproptosis-enhanced low-dose radiotherapy.
Tang H, Lyu M, Quan H … +1 more , Tan ZJ
Colloids Surf B Biointerfaces · 2026 May · PMID 42119355 · Publisher ↗
As a novel form of regulated cell death, cuproptosis holds considerable promise in reversing tumor therapy resistance. However, its application is severely limited by the hypoxic tumor microenvironment (TME), glutathione...
As a novel form of regulated cell death, cuproptosis holds considerable promise in reversing tumor therapy resistance. However, its application is severely limited by the hypoxic tumor microenvironment (TME), glutathione (GSH)-dependent copper chelation, and off-target toxicity. Similarly, conventional low-doses radiotherapy struggles to achieve high treatment efficacy. Herein, we developed exosome-encapsulated CuPt-S hybrid nanoclusters (EM-CPS) to boost enzymatic activity, address these limitations, and enhance low-dose radiotherapy via cuproptosis. To address the problems of insufficient electron transfer and low therapeutic efficacy, sulfur hybridization was employed to engineer abundant copper valence states and ion pairs (Cu⁰/Cu⁺/Cu²⁺ and Pt⁰/Pt²⁺), thereby fundamentally endowing EM-CPS with efficient triple enzyme-mimetic activities (catalase, peroxidase, glutathione oxidase). Meanwhile, to reduce the nonspecific cytotoxicity of inorganic nanoclusters and improve tumor targeting and immune evasion, exosome membranes were strategically engineered onto the nanocluster surfaces via a co-incubation approach, thereby fabricating a novel biomimetic nanoplatform. The nanozyme EM-CPS can significantly modulate TME and influence metabolism. Catalase-like activity alleviated hypoxia by converting H₂O₂ to O₂, promoting lipoylated protein oligomerization. Peroxidase-like activity generated reactive oxygen species to enhance radiosensitivity. Glutathione oxidase-like activity depleted GSH to prevent copper chelation. Notably, low-dose radiotherapy not only causes direct damage to cancer cells but also more significantly serves as a potent inducer of cuproptosis. This biomimetic nanoplatform achieves potent tumor suppression at low radiation doses, offering a precise and safe cancer therapeutic strategy.
Copper-doped carbon dots nanozymes with dual peroxidase/catalase activities for synergistic ROS-mediated ultra-potent MRSA eradication and wound healing.
Bao GM, Zhang QY, Cai ZQ … +11 more , Zhang YY, Zhao K, Liang XY, Idriss MB, Wang MK, Wu JH, Zheng M, He LG, Chen P, Zhao YS, Yuan HQ
Colloids Surf B Biointerfaces · 2026 May · PMID 42119354 · Publisher ↗
Methicillin-resistant Staphylococcus aureus (MRSA) infections threaten global public health, demanding innovative antibiotic-free antibacterial strategies to overcome conventional therapeutic limitations. Herein, copper-...
Methicillin-resistant Staphylococcus aureus (MRSA) infections threaten global public health, demanding innovative antibiotic-free antibacterial strategies to overcome conventional therapeutic limitations. Herein, copper-doped carbon dots (Cu-CDs) were synthesized via a facile one-pot hydrothermal method, featuring dual peroxidase (POD)- and catalase (CAT)-like nanozyme activities. In vitro antibacterial assays demonstrated concentration-dependent MRSA eradication, with minimum bactericidal concentrations (MBCs) of 1.0 μg/mL for Cu-CDs alone and 0.25 μg/mL for Cu-CDs in combination with HO, yielding antibacterial efficacy 2048-fold more potent than vancomycin. Mechanistic studies elucidated a four-stage synergistic bactericidal process: (i) electrostatic adsorption /biofilm disruption; (ii) reactive oxygen species (ROS) -mediated cell membrane damage; (iii) intracellular ·OH generation and Cu-induced bacterial DNA breaks; and (iv) metabolic/antioxidant system collapse with dysregulated drug-resistant (mecA) and cell division genes. Cu-CDs exhibited exceptional biocompatibility (L929 viability >95% at 150 μg/mL; hemolysis <5% at 50 μg/mL). In MRSA-infected mouse wounds, Cu-CDs + HO achieved ∼92% closure by day 7, with intact epidermis and no organ pathology. This work establishes Cu-CDs as a high-performance nanozyme platform for antibiotic-free MRSA therapy, advancing nanozyme clinical translation.
Glycine linker modulates the tunable self-assembly and antibacterial efficacy of octanoyl lipopeptides.
Chen C, Zhang Y, Gu Q … +5 more , Gu C, Zhang X, Jiang X, Song X, Fan X
Colloids Surf B Biointerfaces · 2026 May · PMID 42119353 · Publisher ↗
The linker region of lipopeptides has demonstrated considerable potential in modulating their antibacterial activity. However, a clear consensus regarding the influence of link length on their physicochemical properties...
The linker region of lipopeptides has demonstrated considerable potential in modulating their antibacterial activity. However, a clear consensus regarding the influence of link length on their physicochemical properties and biological activities remains elusive. To address this, we designed a series of octanoyl lipopeptides, CG(VVKK)V-NH (n = 1-3), with varying glycine linker length. Altering the linker length led to distinct physicochemical properties and self-assembly behaviors, enabling an investigation into the structure-property-function relationship. Among the series, CG(VVKK)V-NH exhibited the lowest surface activity and consequently the weakest antibacterial efficacy. In contrast, CG(VVKK)V-NH and CG(VVKK)V-NH displayed high surface activity, and self-assembled into micelles above their critical aggregation concentrations (CACs). Notably, these micelles disassembled upon dilution, facilitating high antibacterial activity through bacterial membrane disruption. Conversely, the micelles formed by CG(VVKK)V-NH were exceptionally stable, allowing them to attach on bacterial surface but not permeate bacterial membrane, leading to low antibacterial activity. Thus, we demonstrate that the incorporating glycine linkers modulates the self-assembly behavior of octanoyl lipopeptides: increased linker length promotes micelle disassembly and enhances antibacterial activity. This study offers deeper insights into the antibacterial mechanisms of self-assembling lipopeptides, facilitating the future exploration of their biomedical applications.
Biomimetic nanozyme mitigates thermal damage in photothermal therapy by targeted disruption of tumor metabolism.
Liu T, Zhang Y, Liu J … +3 more , Wang C, Mao H, Su X
Colloids Surf B Biointerfaces · 2026 May · PMID 42114243 · Publisher ↗
Thermal damage to surrounding healthy tissues poses a challenge in photothermal therapy (PTT) due to the limited targeting efficiency. Here, we report the development of the membrane-functionalized low-temperature photot...
Thermal damage to surrounding healthy tissues poses a challenge in photothermal therapy (PTT) due to the limited targeting efficiency. Here, we report the development of the membrane-functionalized low-temperature photothermal nanozymes M@Au@ZPC (MAZ), thereby mitigating the adverse effects of thermal spillage through simultaneously increasing targeting distribution and decreasing photothermal temperature. The coating of the tumor cell membrane significantly enhanced the accumulation of MAZ at the target site by exploiting the homologous targeting capability of membranes. Furthermore, the catalytic consumption of glucose by Au nanozymes at the tumor site results in the inhibition of ATP production within tumor cells, which subsequently downregulates the expression of heat shock proteins, significantly diminishing the thermotolerance of the tumor cells. As a result, tumor ablation can be effectively achieved at lower temperatures, thereby minimizing the potential for thermal damage. Both in vitro and in vivo experimental results indicated that MAZ nanoparticles can facilitate tumor ablation through targeted low-temperature photothermal therapy. This study informs new therapeutic strategies to decrease adverse effects of thermal spillage in PTT.
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