Colloids Surf B Biointerfaces [JOURNAL]
Sun
200 papers
Plasma etching elevates iohexol-loading capacity of poly(p-dioxanone) for X-ray opaque cardiac occluders.
Lyu H, Liao H, Ruan Q … +8 more , Mo S, Fu RKY, Wu Y, Liu P, Tai F, Kang L, Jiang H, Chu PK
Colloids Surf B Biointerfaces · 2026 May · PMID 42114242 · Publisher ↗
Structural heart diseases are one of the leading health issues. While polydioxanone (PPDO) cardiac occluders can treat this condition, their lack of X-ray visibility makes it challenging to determine device placement dur...
Structural heart diseases are one of the leading health issues. While polydioxanone (PPDO) cardiac occluders can treat this condition, their lack of X-ray visibility makes it challenging to determine device placement during surgery, thereby increasing the risk of complications. Herein, plasma etching (PE) is performed to fabricate micro/nanostructures and introduce active groups on the surface of PPDO occluders, increase the loading of the iohexol (IHX) contrast agent, and prevent rapid detachment. The plasma conditions are optimized to achieve maximum surface roughness. Experimental results show that after plasma etching for 30 min, the PPDO surface can be loaded with up to 14.5 mg/cm. After immersion in the simulated body fluid for 4 h, the grayscale value of the digital subtraction angiography for the IHX-loaded PPDO remains below 100. The PPDO-PE-IHX also exhibits sufficient in vivo X-ray imaging capacity and biosafety. Plasma treatment can improve X-ray imaging without compromising mechanical strength and maintaining the main chemical composition of the occluder, consequently improving diagnostics and success rates in interventional cardiac surgeries.
Microscopic mechanisms of helical protein unfolding under thermal and confinement effects.
He J, He Y, Yu M … +2 more , Sha J, Si W
Colloids Surf B Biointerfaces · 2026 May · PMID 42114241 · Publisher ↗
Protein folding and unfolding remain central problems in biological sciences, yet a unified understanding of native folding mechanisms is still lacking due to structural diversity and complex folding pathways. In this wo...
Protein folding and unfolding remain central problems in biological sciences, yet a unified understanding of native folding mechanisms is still lacking due to structural diversity and complex folding pathways. In this work, molecular dynamics simulations were employed to investigate the high-temperature unfolding behavior of a helical protein. The results reveal that unfolding is initiated preferentially in regions characterized by weaker residue-residue interactions, thereby predefining the unfolding pathway at the molecular level. Comparative simulations further demonstrate that confinement within a silicon nitride nanopore significantly accelerates protein unfolding through electrostatic interactions with the pore surface, while simultaneously suppressing refolding. Beyond confinement effects, the chemical environment of the solution plays a decisive regulatory role. Monovalent cations with small hydration shells facilitate unfolding, whereas high electrolyte concentrations or multivalent ions enhance conformational stability via electrostatic screening. With respect to chemical denaturants, urea promotes unfolding in a monotonic manner, while guanidine hydrochloride exhibits a dual effect, accelerating unfolding at moderate concentrations but stabilizing protein conformations at high concentrations due to dominant screening effects. Overall, this study provides a microscopic picture of how temperature, spatial confinement, and solution chemistry cooperatively regulate protein conformational dynamics, offering new insights into the physical basis of protein folding and stability.
Fabrication of polymeric nanostructures with high aspect ratio for cell differentiation.
Huang L, Li Y, Kontziampasis D … +3 more , Wu W, Jiang B, Zhou M
Colloids Surf B Biointerfaces · 2026 May · PMID 42114240 · Publisher ↗
The regulation of stem cell fate by using the physicochemical signals is a hot research topic in tissue engineering. Compared to biochemical growth factors, mechanical stimulation such as surface nanostructures can remod...
The regulation of stem cell fate by using the physicochemical signals is a hot research topic in tissue engineering. Compared to biochemical growth factors, mechanical stimulation such as surface nanostructures can remodel the microenvironment in which cells are located to via their own physical properties. To fabricate bioresorbable nanostructures with an optimal design for cell differentiation, a robust and versatile process chain needs to be developed. In this work, a process chain combining self-assembled mold inserts and injection molding was proposed to fabricate nanopillars with different aspect ratios as the substrates for cell culture. Herein, the optimal parameters affecting the anodic oxidation process for mold inserts and the injection molding process for nanopillar were investigated. The injection-molded nanopillars were then applied to the differentiation experiments of bone marrow mesenchymal stem cells (BMSCs). The results showed that nanopillar structures with different aspect ratios were precisely fabricated through the process chain. Nanopillars had a significant osteogenic differentiation inducing effect on BMSCs, and the inducing effect became stronger with the increase in the aspect ratio. Compared with the addition of osteogenic inducers, nanopillar substrates exhibited a better effect in inducing differentiation and provided an alternative protocol for differentiation induction.
Fabrication and evaluation of a peptide-magnesium bifunctional coating on 3D printed porous tantalum scaffolds for enhanced bone regeneration.
Xie Q, Jiao H, Cao F … +7 more , Ge B, Yi P, Song L, Wang H, Liu L, Zhao D, Li J
Colloids Surf B Biointerfaces · 2026 May · PMID 42114239 · Publisher ↗
Traditional bone repair strategies face significant limitations. This study proposes a novel strategy by constructing a "QK peptide-magnesium" (QK-Mg) bifunctional coating on selective laser melting (SLM)-fabricated, per...
Traditional bone repair strategies face significant limitations. This study proposes a novel strategy by constructing a "QK peptide-magnesium" (QK-Mg) bifunctional coating on selective laser melting (SLM)-fabricated, personalized porous tantalum (pTa) scaffolds to overcome these issues. A Mg ions sustained-release coating (comprising magnesium calcium phosphate via hydrothermal treatment and magnesium citrate via vacuum impregnation) was first constructed on the scaffold surface. Subsequently, an angiogenic polypeptide (VEGF-mimetic QK peptide) was covalently grafted onto the coating via a polydopamine-mediated reaction. Comprehensive in vitro characterization confirmed the successful formation and chemical composition of the coating. It exhibited a bimodal, sustained release profile of Mg and a pH-responsive release of the QK peptide. Biological evaluations demonstrated that the QK-Mg-pTa scaffolds significantly enhanced the proliferation, adhesion, and spreading of MC3T3-E1 pre-osteoblasts compared to uncoated controls. These scaffolds notably upregulated osteogenic differentiation, as evidenced by increased ALP activity, enhanced mineralized nodule formation, and elevated expression of osteogenic genes (OCN, Runx2, COL-1). Furthermore, the QK-Mg coating promoted robust angiogenesis, as confirmed by enhanced CD31 expression, improved tube formation, and increased migration of C166 vascular endothelial cells. The synergistic effect of Mg ions, known to activate osteogenic pathways and modulate macrophage polarization, and the QK peptide, which mimics VEGF to stimulate angiogenesis, overcomes the limitations of single-functional modifications. This engineered coating endows 3D-printed pTa scaffolds with enhanced osteogenic, angiogenic, and immunomodulatory capabilities, offering a promising solution for the clinical repair of complex bone defects.
Quaternary tannic acid-based colorless coatings with outstanding antimicrobial properties for clear aligners.
Chen S, Chen Z, Cai J … +4 more , Chen L, Cai J, Yuan W, Xu L
Colloids Surf B Biointerfaces · 2026 May · PMID 42114238 · Publisher ↗
BACKGROUND: Surface degradation of clear aligners promotes bacterial adhesion and biofilm formation, which increases the risk of enamel demineralization and compromises aesthetics. This study aimed to improve the antimic...
BACKGROUND: Surface degradation of clear aligners promotes bacterial adhesion and biofilm formation, which increases the risk of enamel demineralization and compromises aesthetics. This study aimed to improve the antimicrobial performance of clear aligners by applying a hydrophobic quaternary tannic acid (QTA) coating that does not alter the mechanical or optical properties. METHODS: Aligners were dip-coated with QTA, and the coating characteristics were evaluated using scanning electron microscopy (SEM)-energy dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and Fourier transform infrared analysis. Properties including water sorption, mechanics, optical transmittance, and color stability were evaluated. Antimicrobial efficacy was tested using mono- and polymicrobial biofilm models with colony forming unit (CFU) counts, crystal violet staining, and SEM imaging. Biocompatibility was assessed using a cell-counting kit-8, hemolysis tests, and a hamster oral-irritation model. RESULTS: QTA was successfully grafted onto aligners without compromising their mechanical or optical properties. Coated aligners exhibited strong antimicrobial activity, with > 99% and 82.9% (p < 0.05) reductions in Streptococcus mutansand Candida albicans CFU counts, respectively. Furthermore, they effectively inhibited mono- and polymicrobial biofilm formation. The antimicrobial effects remained stable after exposure to artificial saliva and storage for 3 months. Biocompatibility was confirmed based on low cytotoxicity, minimal hemolysis, and no mucosal irritation in vivo. CONCLUSION: QTA-based coatings endow clear aligners with potent and sustained antimicrobial activity. Moreover, the favorable biocompatibility and preserved functional properties of QTA-coated aligners suggest a strong potential for clinical orthodontic applications.
Dual-mode ECL/SERS ratiometric sensing of 3CLpro via enzyme-gated, entropy-driven DNA assembly on CsPbBr₃@PDA@Au.
Zhang C, Ren H, Luo J … +5 more , Liang L, Wei Y, Luo Q, Zhang K, Liao X
Colloids Surf B Biointerfaces · 2026 May · PMID 42114237 · Publisher ↗
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 3C-like protease (3CLpro) is a key viral enzyme responsible for polyprotein processing and viral replication, making it an important functional biomarker for i...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 3C-like protease (3CLpro) is a key viral enzyme responsible for polyprotein processing and viral replication, making it an important functional biomarker for infection analysis and antiviral evaluation. Herein, we report a same-interface dual-mode electrochemiluminescence/surface-enhanced Raman scattering (ECL/SERS) biosensor for sensitive 3CLpro detection based on a CsPbBr3@PDA@Au nanocomposite and an entropy-driven DNA assembly strategy. In this design, 3CLpro specifically cleaves a DNA-peptide-DNA precursor to generate an intermediate DNA, which subsequently triggers interfacial strand-displacement assembly and recruits the Fc-labeled reporter strand to the electrode surface. This target-induced interfacial reconstruction leads to opposite signal changes, namely ECL attenuation and SERS enhancement, thereby enabling ratiometric quantification through I/I. The integrated dual-mode platform combines the high ECL activity of CsPbBr, the stabilizing and functional role of PDA, and the plasmonic/DNA-anchoring properties of Au nanoparticles, allowing 3CLpro activity to be transduced into a robust and self-validated analytical output. The proposed biosensor exhibited high sensitivity, good selectivity, satisfactory reproducibility, and favorable stability. According to the standard 3σ criterion, the limit of detection was calculated to be 2.44 aM. Moreover, the ratiometric readout improved analytical robustness by partially compensating for common-mode fluctuations associated with single-channel measurements. The method was further successfully applied to clinical samples, demonstrating its potential for reliable 3CLpro analysis in complex matrices. Overall, this work provides a dual-mode ratiometric biosensing strategy for 3CLpro that integrates activity-based target recognition, entropy-driven interfacial DNA reconstruction, and same-interface ECL/SERS readout, offering a promising platform for sensitive viral protease analysis and practical bioassay applications.
Heat-amplified catalytic BP-CuMOF heterojunction nanozyme for antibiotic-free treatment of drug-resistant infections.
Xu D, Shao W, Wang C … +2 more , Ding X, Hu S
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42107301 · Publisher ↗
Eradicating deep-seated biofilm infections remains a formidable challenge in clinical settings due to the dense extracellular polymeric substance (EPS) barrier and the hypoxic microenvironment. Accordingly, there is an u...
Eradicating deep-seated biofilm infections remains a formidable challenge in clinical settings due to the dense extracellular polymeric substance (EPS) barrier and the hypoxic microenvironment. Accordingly, there is an urgent need to develop nanozyme systems that integrate potent antibacterial efficacy, deep tissue penetration capability, and high biosafety. Herein, a heterojunction nanocatalytic platform is constructed via the in-situ growth of a copper-based metal-organic framework (Cu-MOF) on black phosphorus (BP) nanosheets. Benefiting from the facilitated interfacial charge separation and the localized thermal effect of BP, the resulting nanozyme exhibits a high photothermal conversion efficiency of 54.2% and markedly enhanced peroxidase-like catalytic activity under near-infrared (NIR) irradiation. In the presence of trace amounts of hydrogen peroxide (HO) and NIR laser exposure, BP-CuMOF demonstrates robust biocatalytic antibacterial performance, achieving antibacterial efficiencies of 99.7% against methicillin-resistant Staphylococcus aureus (MRSA) and effective biofilm eradication in vitro. Furthermore, this nanozyme efficiently eliminates MRSA infections in wound models and significantly accelerates wound healing in vivo, while exhibiting excellent biosafety. Collectively, this work presents a photothermally enhanced heterojunction nanozyme as an efficient and safe therapeutic strategy for the treatment of deep-seated bacterial infections, offering a promising approach for MRSA management.
ROS-responsive hydrogel for treating pulpitis: Localized immunometabolic regulation by dimethyl itaconate promotes reparative dentin formation.
Tsai IC, Cai L, Tian C … +5 more , Ni Z, Ding W, Li Y, Li W, Xu Q
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42107300 · Publisher ↗
Pulpitis is a common inflammatory disease of the dental pulp in which excessive inflammation and oxidative stress severely compromise the regenerative capacity of dental pulp stem cells (DPSCs), posing a major challenge...
Pulpitis is a common inflammatory disease of the dental pulp in which excessive inflammation and oxidative stress severely compromise the regenerative capacity of dental pulp stem cells (DPSCs), posing a major challenge for successful vital pulp therapy. Herein, we report an immunometabolic strategy that combines dimethyl itaconate (DMI) with a reactive oxygen species (ROS)-responsive injectable hydrogel to modulate the inflammatory microenvironment and promote functional pulp regeneration. We demonstrated that DMI effectively suppressed lipopolysaccharide (LPS)-induced inflammatory cytokine expression and ROS accumulation in DPSCs. Notably, DMI did not directly enhance odontogenic differentiation under inflammatory conditions; instead, it indirectly restored the odontogenic potential of DPSCs by attenuating macrophage-mediated inflammation. To enable localized and sustained delivery of DMI, a dual-crosslinked SADA/CMBA hydrogel was developed based on dynamic boronic ester bonds and ionic coordination, allowing ROS-triggered drug release while maintaining structural integrity and biocompatibility. In vitro studies confirmed the hydrogel's favorable cytocompatibility, anti-inflammatory and antioxidant effects. Furthermore, in an LPS-induced rat pulpitis model, the DMI-loaded hydrogel significantly reduced pulpal inflammation and facilitated reparative dentin formation at the pulp exposure site. Collectively, this study introduces an immunometabolic and microenvironment-responsive therapeutic design for pulpitis treatment and highlights the potential of ROS-adaptive biomaterials to enhance the outcomes of vital pulp therapy.
Cu²⁺ triggers lipid phase separation in anionic membranes via a bimodal interfacial mechanism.
Zhang J, Wang Y, Zeng J … +5 more , Tang C, Li Y, Huang Y, Hu J, Lü J
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42107299 · Publisher ↗
The cellular membrane serves as the primary interface for sensing environmental cues, yet how it decodes the concentration-dependent toxicity of essential metal ions like copper remains a fundamental question. Here, we u...
The cellular membrane serves as the primary interface for sensing environmental cues, yet how it decodes the concentration-dependent toxicity of essential metal ions like copper remains a fundamental question. Here, we unveil a bimodal molecular switching mechanism by which Cu²⁺ reorganizes anionic lipid membranes, suggesting that the cell membrane could act as a sensor for copper concentration. Using an integrative biophysical approach on phosphatidylcholine/phosphatidylglycerol membranes, we demonstrate that at low concentrations, Cu²⁺ binds individually to anionic lipids, reorienting headgroups and priming the membrane for separation (Stage I). Crucially, beyond a critical threshold, adjacent membrane-bound Cu²⁺ ions form metal-metal bonds, creating rigid [PG-Cu-Cu-PG] bridges that act as molecular clamps (Stage II). This cooperative bridging event forcefully squeezes lipids together, driving extensive phase separation and the formation of Cu-rich domains with markedly enhanced thermodynamic stability. We provide evidence through atomic force microscopy, X-ray scattering, and calorimetry, complemented by the spectroscopic signature of ESR-silent Cu-Cu pairs. This bimodal switch model provides a physicochemical basis for copper's dual biological identity, with direct implications for understanding its antimicrobial mechanism and its potential role in neurotoxicity. Our findings establish a new paradigm for how transition metal coordination chemistry can programmatically control membrane architecture.
Collagen films with controlled surface topology improve corneal cell migration.
Nashchekina Y, Sirotkina M, Inozemtceva A … +5 more , Kriger D, Temnikov O, Prasolov N, Nashchekin A, Mikhailova N
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42107298 · Publisher ↗
Corneal damage is a significant problem in modern ophthalmology, and the repair of this damage through the transplantation of collagen films has become an attractive and promising approach in regenerative ophthalmology....
Corneal damage is a significant problem in modern ophthalmology, and the repair of this damage through the transplantation of collagen films has become an attractive and promising approach in regenerative ophthalmology. We have developed a method for creating collagen films using simple and accessible techniques that enhance the migration of corneal cells. Our research has focused on investigating the effects of variables such as the concentration of chloride anions in collagen solutions and pre-cooling on the properties of these films. Using turbidity measurements, we found that pre-incubation of collagen solutions at low temperatures promotes the formation of fibrillar structures. Atomic force microscopy confirmed these findings, revealing an increase in fibrillar collagen content on the surface of the films. The proposed conditions for film formation contribute to an increase in the rate of migration of corneal cells. These films represent a promising product for use in corneal regenerative medicine.
Pectin/tamarind gum-based edible active films reinforced with supramolecularly self-assembled apigenin nanocrystals for shelf-life extension of strawberries.
Qin R, Xue J, Xu W … +3 more , Wang H, Jiang L, Rhim JW
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42105600 · Publisher ↗
Apigenin nanocrystals (AN) were synthesized through supramolecular self-assembly and incorporated into a pectin/tamarind gum (PTG) matrix to fabricate edible bio-based nanocomposite films. Scanning electron microscopy (S...
Apigenin nanocrystals (AN) were synthesized through supramolecular self-assembly and incorporated into a pectin/tamarind gum (PTG) matrix to fabricate edible bio-based nanocomposite films. Scanning electron microscopy (SEM) confirmed that AN exhibits a rod-like nanostructure. Both simulations and experimental results demonstrated that supramolecular interactions are the primary driving force behind AN self-assembly. The nanocrystals showed excellent biocompatibility. Structural characterization revealed that AN was uniformly dispersed within the PTG matrix. It formed strong intermolecular interactions and a dense three-dimensional network. As a nanofiller, AN significantly enhanced the mechanical properties of the films. The tensile strength (TS) reached 9.89 MPa, and the elongation at break (EAB) reached 56.83%. These values outperform those of films containing non-self-assembled Apigenin (Ap) (TS = 8.52 MPa; EAB = 51.61%). In addition, AN improved the thermal stability, UV-blocking ability, and water vapor barrier properties of the PTG films. Its higher specific surface area also provided superior antioxidant and antibacterial activities compared with untreated Ap. In strawberry preservation tests, PTG films containing AN extended the shelf life to eight days.
A natural COX-2 inhibitor-integrated nanoplatform for TNBC immunotherapy: Mn-PC-PTX synergizes chemotherapy and immune activation.
Wang H, Lin S, Zhou J … +8 more , Bi J, Wang Y, Li X, Yang Q, Wu S, Qi X, Shen S, Cao J
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42102776 · Publisher ↗
Chemotherapy-induced pyroptosis has emerged as a promising strategy to initiate antitumor immunity; however, its clinical translation remains limited by intrinsic immunosuppressive feedback. In particular, paclitaxel (PT...
Chemotherapy-induced pyroptosis has emerged as a promising strategy to initiate antitumor immunity; however, its clinical translation remains limited by intrinsic immunosuppressive feedback. In particular, paclitaxel (PTX)-triggered pyroptosis concurrently upregulates the COX-2/PGE/PD-L1 axis, constraining immune amplification and rendering pyroptosis an inherently unstable immunogenic event. Here, we report a multifunctional nanoplatform (Mn-PC-PTX) designed to resolve this contradiction by synchronizing immunogenic cell death with immune brake release and innate immune amplification. Phycocyanin (PC) is deliberately integrated as a bioactive carrier to selectively suppress COX-2-mediated immunosuppression during PTX-induced pyroptosis, thereby enabling effective antigen presentation and T-cell priming. Concurrently, Mn potentiates cGAS-STING signaling in response to pyroptosis-derived cytosolic DNA, amplifying type I interferon production and innate immune activation. Our results identify COX-2 suppression as a prerequisite for productive pyroptosis-driven immunity, while STING activation functions as an essential amplifier rather than an independent initiator. By hierarchically coupling cell death induction, immune brake release, and innate immune sensing within a single nanosystem, Mn-PC-PTX converts transient chemotherapy-induced inflammation into durable systemic antitumor immunity, effectively inhibiting tumor growth, metastasis, and rechallenge. This work establishes a bioactive carrier-based design principle for overcoming the intrinsic immunological limitations of immunogenic chemotherapy and advancing chemo-immunotherapy-oriented biomaterials.
A nanozyme-engineered implant surface for sequential photodynamic antibacterial and cytoprotective functions.
Yan S, Hu N, Wu B … +4 more , Yang M, Wei J, Wang G, Ma H
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42096975 · Publisher ↗
Addressing the dual challenges of bacterial infection and poor osseointegration in titanium implants is hindered by the "bactericidal vs. osteogenic" conflict. Here, we present a hierarchical nano-architecture with an "a...
Addressing the dual challenges of bacterial infection and poor osseointegration in titanium implants is hindered by the "bactericidal vs. osteogenic" conflict. Here, we present a hierarchical nano-architecture with an "attack-defense" strategy to achieve temporal synergy. We first constructed an NIR-responsive TiO optical superstructure for potent photodynamic killing. On top of this, a CoMnO nanozyme layer with high catalase-like activity was anchored to provide a defensive function. Upon NIR irradiation, the surface first unleashes a powerful bactericidal ROS burst, which is then rapidly scavenged by the nanozyme layer. This process not only ensures high antibacterial efficacy but also transforms the oxidative microenvironment into one conducive to healing. Consequently, osteoblasts cultured on the surface were protected from oxidative damage and exhibited significant upregulation of osteogenic differentiation markers. By temporally decoupling these antagonistic functions, our work provides a novel pathway for developing next-generation intelligent implants.
Development of novel, non-toxic PEG-albumin-AuNPs to promote macrophage migration and polarization.
Haripriya S, Hariharan G, Arivarasan A … +3 more , Seenivasagan R, Yenn TW, Pandian SRK
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42096974 · Publisher ↗
Polyethylene glycol (PEG)-albumin functionalized gold nanoparticles (PEG-albumin-AuNPs) were synthesized via a green, one-pot reduction approach using a fruit bioflavonoid, rutin, as a reducing and stabilizing agent. PEG...
Polyethylene glycol (PEG)-albumin functionalized gold nanoparticles (PEG-albumin-AuNPs) were synthesized via a green, one-pot reduction approach using a fruit bioflavonoid, rutin, as a reducing and stabilizing agent. PEG-albumin-AuNPs were evaluated for their immunomodulatory potential in vitro. Surface plasmon resonance analysis confirmed nanoparticle formation. Comprehensive physicochemical characterization using zeta potential, FT-IR, XRD, and TEM demonstrated enhanced colloidal stability, crystallinity, and uniform spherical morphology. The average particle size of AuNPs ranged from 5 to 20 nm and increased to 15-20 nm following PEG-albumin functionalization. A high positive zeta potential (+49.6 mV) indicated high colloidal stability suitable for long-term storage and biomedical applications. The immunomodulatory potential of PEG-albumin-AuNPs was assessed using murine macrophage (RAW 264.7) cells. MTT assay identified 75 µg/mL as the optimal non-cytotoxic concentration. NBT reduction and LDH release assays confirmed minimal toxicity. Functional analyses, including cell-sprouting and migration assays, revealed enhanced macrophage activation, indicating a shift toward an anti-inflammatory phenotype. Overall, PEG-albumin functionalization greatly enhances the physicochemical stability and biological activity of gold nanoparticles. These findings support the potential of PEG-albumin-AuNPs as biocompatible immunomodulatory nanomaterials, although further mechanistic and in vivo validation is required.
A review of nucleic acid aptamer-functionalized metal-organic frameworks for cancer biomarker detection.
Li Q, Meng G, Ga L … +3 more , Su A, Yu L, Ai J
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42096973 · Publisher ↗
Timely detection of cancer is an important step towards better cure rates, and patient prognosis, and the sensitive detection of biomarkers is a significant milestone in this direction. Biosensing uses of metal-organic f...
Timely detection of cancer is an important step towards better cure rates, and patient prognosis, and the sensitive detection of biomarkers is a significant milestone in this direction. Biosensing uses of metal-organic frameworks (MOFs) due to the high specific surface area, tunable pore size, and excellent functionalization capability have unique benefits. Through the combination of high-affinity nucleic acid aptamers and MOFs, the resulting sensors could have a better sensitivity and sensing performance. This review is a summary of the research development in the last five years on aptamer-MOF composite systems in detecting cancer biomarkers. Particular emphasis is placed on immobilization strategies, signal transduction mechanisms, and the development of electrochemical、optical、photoelectrochemical、 electrochemiluminescent and other emerging sensing platforms, as well as their use in the detection of biomarkers, including ATP、CEA、CA15-3 and ctDNA. Lastly, the performance, constraints, and prospects of aptamer-MOF biosensors are addressed, including aptamer-MOF sensor sensitivity, aptamer-MOF sensor selectivity, aptamer-MOF sensor reproducibility, and aptamer-MOF sensor translatability.
Gluten content, composition, and structural characteristics dominate chinese steam bun quality: Insights from varied wheat varieties.
Sharafeldin S, Akbar T, Zhu Y … +5 more , Liangxing Z, Sang L, Chen R, Dai Z, Qun S
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42096972 · Publisher ↗
Chinese steam bun (CSB), a 1,700-year-old staple food, remains highly valued for its nutritional and sensory qualities. Although gluten content influences CSB quality, the roles of gluten composition, structural organiza...
Chinese steam bun (CSB), a 1,700-year-old staple food, remains highly valued for its nutritional and sensory qualities. Although gluten content influences CSB quality, the roles of gluten composition, structural organization, and intermolecular interactions remain less clearly defined. This study investigated these factors using seven wheat varieties, namely Canadian Spring (CS), Yongliang No.4 (Y4), You-mai 02-1 (Y2), Local Xinpu (LX), Hard Australian (HA), White Australian (WA), and French Wheat (FR), selected to capture gluten variability across diverse geographic origins. Wet gluten content (22.7%-37.0%, w/w) showed a non-linear relationship with specific volume, with moderate levels (32.3-33.1 g/100 g flour) being most favorable for dough expansion and bun volume. Glutenin content (r = 0.73) and the gliadin-to-glutenin ratio (r = 0.50) were positively correlated with specific volume. Secondary structure analysis showed that elevated antiparallel β-sheet and β-turn contents were positively associated with specific volume (r = 0.54 and r = 0.30, respectively), indicating the importance of a balanced gluten network with both stability and flexibility. In contrast, higher free sulfhydryl (SH) contents in WA and FR were associated with weaker gluten networks and lower specific volume (r = -0.44). Higher absolute zeta potential values, particularly in HA, were associated with a more organized gluten network and improved expansion performance (r = 0.49). These findings demonstrate that CSB quality depends not only on gluten quantity but also, more importantly, on gluten composition, structural characteristics, and intermolecular interactions.
Triple-targeted artificial extracellular vesicles for enhanced glioma therapy via synergistic chemomagnetic penetration of the blood-brain barrier.
Yi G, Huang Z, Hua Y … +6 more , Wu S, Yu H, Wu J, Zhao Z, Yang Y, Liu Y
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42090930 · Publisher ↗
Malignant glioma remains one of the most aggressive and therapy-resistant brain tumors, with limited treatment options and a poor prognosis. Overcoming the blood-brain barrier (BBB) is a major challenge for effective dru...
Malignant glioma remains one of the most aggressive and therapy-resistant brain tumors, with limited treatment options and a poor prognosis. Overcoming the blood-brain barrier (BBB) is a major challenge for effective drug delivery. Here, we developed a triple-targeted nanodelivery system, denoted BCNU-FeSeH@EV, which integrates magnetic guidance, chemotactic homing, and receptor-mediated transport for enhanced brain penetration and glioma treatment. The system consists of carmustine (BCNU)-loaded, hyaluronic acid-modified superparamagnetic FeO nanoparticles with a mesoporous selenium shell (FeSeH NPs), which are further encapsulated within lactoferrin-engineered extracellular vesicles (EV) derived from bone marrow mesenchymal stem cells. In vitro and in vivo studies demonstrated that BCNU-FeSeH@EV exhibited excellent BBB-crossing ability under magnetic field exposure, targeted accumulation in glioma tissues, and pH-responsive drug release within the tumor microenvironment. Selenium and BCNU act synergistically to induce reactive oxygen species generation, DNA damage, and apoptosis, leading to potent antiglioma efficacy. Importantly, the system showed high biocompatibility and negligible systemic toxicity. This work presents a robust and safe strategy for targeted glioma therapy via a multifunctional extracellular vesicle-based platform.
Mechanism underlying the tolerance of lipase immobilized in porous organic frameworks in organic solvents.
Pei R
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42090929 · Publisher ↗
Enzymes represent promising biocatalysts; however, their industrial application is often limited by instability under harsh conditions, particularly their low tolerance to organic solvents. Although immobilization within...
Enzymes represent promising biocatalysts; however, their industrial application is often limited by instability under harsh conditions, particularly their low tolerance to organic solvents. Although immobilization within porous organic frameworks (POFs) offers a potential solution, systematic investigations remain limited. In this work, Aspergillus niger lipase (ANLP) was immobilized in three POFs (ZIF-8, COF-LZU1, and HOF-101), and the tolerance of these composites was evaluated in six common organic solvents, including n-hexane and dichloromethane. The underlying mechanisms governing solvent tolerance were further investigated. Free ANLP exhibited progressive aggregation with increasing solvent polarity, resulting in reduced activity, whereas immobilization within POFs prevented aggregation through spatial confinement. In addition, hydrophobic carriers promoted interfacial interactions with ANLP that induced opening of the catalytic "lid," thereby enhancing substrate accessibility and enzymatic activity. In contrast, polar solvents promoted lid closure and reduced activity. Hydrophobic interfaces, therefore, contribute to stabilizing the active conformation of ANLP and counteracting the lid-closing effects of polar solvents. Overall, the organic solvent tolerance of immobilized ANLP is governed by both the porosity and hydrophobicity of the POF host. This study provides systematic insight into enzyme-POF-solvent interactions and offers perspectives for the rational design of efficient enzyme catalytic systems tailored to specific industrial environments.
Muscle-inspired, high-speed hydrogel based on magnetic carrageenan/mesona gum interpenetrating network for integrated actuation and strain sensing in motion monitoring.
Fu Y, Yang C, Wan Z … +4 more , Liu P, Zhu D, Chen X, Wei R
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42090928 · Publisher ↗
The development of high-performance magnetic carrageenan/mesona gum (CMG) hydrogel-based soft actuators that integrate rapid response, autonomous motion, and real-time sensing capabilities remains a significant challenge...
The development of high-performance magnetic carrageenan/mesona gum (CMG) hydrogel-based soft actuators that integrate rapid response, autonomous motion, and real-time sensing capabilities remains a significant challenge. Inspired by the muscle fiber structure, a hamburger-like conductive composite is presented that achieved a synergistic combination of high biocompatibility, high-speed, integrated magnetic actuation and self-sensing functionality via an eco-friendly fabrication strategy. The magnetic actuation hydrogel exhibits excellent flexibility and magnetically actuated capability attributed to the gradient distribution of magnetic particles (MPs)@multi-walled carbon nanotube (MWCNTs) within the CMG interpenetrating network. Meanwhile, benefiting from the satisfied electrochemical performance, the MWCNTs/sodium alginate (SA) electrodes on both sides enhance the electrical responsiveness and reliability to external stimuli. The developed device demonstrates satisfied biocompatibility, high strain sensitivity (gauge factor of 3.06), fast response (107 ms), remarkable cycling durability (6000 cycles), and rapid magnetic actuation speed of 26.7°/s. The core achievement is that the co-localization of actuation and sensing within a monolithic structure, which allows simultaneous actuated deformation and real-time motion monitoring. This has been demonstrated in bio-inspired systems, including a gripper capable of detecting its own position and grip, and a fish-like actuator that can monitor its own movement. Therefore, this work offers a novel strategy and inspiration for designing intelligent flexible actuation with self-feedback loops, displaying great potentials in next-generation wearable electronics, soft bionic robots, and human-machine interaction.
Bioinspired adhesive hydrogel incorporated with PDA@CuMOF for infected wound therapy.
Chen H, Shen Z, Shen D … +3 more , Wang X, Mei L, Yang B
Colloids Surf B Biointerfaces · 2026 Sep · PMID 42090927 · Publisher ↗
Infected wounds pose a significant clinical challenge due to bacterial colonization, excessive inflammation, and impaired tissue regeneration. Inspired by the adhesive properties of mussel foot proteins, this study devel...
Infected wounds pose a significant clinical challenge due to bacterial colonization, excessive inflammation, and impaired tissue regeneration. Inspired by the adhesive properties of mussel foot proteins, this study developed a novel multifunctional wound dressing by incorporating polydopamine (PDA)-modified copper-based metal-organic framework (CuMOF) into a polyethylene glycol (PEG) hydrogel network. The synthesized PEG/PDA@CuMOF (PPCM) hydrogel combines the extracellular matrix (ECM)-mimetic properties of PEG with the robust wet adhesion, antimicrobial, and pro-regenerative activities of CuMOF. Comprehensive characterization confirmed successful PDA coating on CuMOF and covalent integration into the hydrogel, which exhibited excellent porosity and swelling capacity. In vitro assessments demonstrated outstanding biocompatibility, significant promotion of cell proliferation and migration, and powerful antibacterial efficacy against both methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). In a rat model of full-thickness MRSA-infected wounds, the PPCM significantly accelerated wound closure, enhanced collagen deposition, promoted neovascularization, and effectively reduced bacterial burden, all while maintaining excellent systemic biocompatibility. These findings highlight the PPCM hydrogel as a promising multifunctional platform for advanced infected wound management.
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