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Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
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200 papers
[Association Study of Parity With Adverse Pregnancy Outcomes].
Yao J, Guo X, Dong X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021889 · Full text
OBJECTIVE: To investigate the relationship between parity and adverse pregnancy outcomes. METHODS: Keyword searches were conducted by 'parity', followed by random sampling to select 1200 patients: 400 from each of the fi...
OBJECTIVE: To investigate the relationship between parity and adverse pregnancy outcomes. METHODS: Keyword searches were conducted by 'parity', followed by random sampling to select 1200 patients: 400 from each of the first, second and third childbirths, designated as Groups 1, 2 and 3, respectively. A retrospective cohort study examined pregnancy outcomes across these three groups. Logistic regression was used to estimate the association between parity and pregnancy outcomes after adjusting for other confounding factors. We assessed the impact of parity on adverse pregnancy outcomes and the corresponding risk coefficients using receiver operating characteristic (ROC) curves and the area under the curve (AUC). RESULTS: Multiparity (parity ≥ 2) is a risk factor for preterm birth (odds ratio [OR] = 1.602, 95% CI: 1.181-2.173), perinatal anemia (OR = 1.468, 95% CI: 1.099-1.963), and uterine rupture (OR = 2.752, 95% CI: 1.261-6.007). It is a protective factor against low birth weight (OR = 0.564, 95% CI: 0.321-0.992), meconium-stained amniotic fluid (OR = 0.556, 95% CI: 0.418-0.739), and fetal distress (OR = 0.171, 95% CI: 0.080-0.365) ( < 0.05). CONCLUSION: Increased parity is associated with a higher risk of certain adverse pregnancy outcomes, so it is important to be vigilant about the increased risk of specific complications, such as uterine rupture. Strictly controlling the indications for cesarean section and maintaining reasonable weight gain during pregnancy are effective measures to prevent adverse pregnancy outcomes in multiparous women.
[Research Progress of Peripheral Immune Tolerance in Oral Diseases].
Wen K, Zhang P, Peng X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021888 · Full text
Peripheral immune tolerance serves as a core mechanism for maintaining immune homeostasis within the body. Regulatory T cells (Tregs) are the key cells responsible for performing this function. Through cell-contact-depen...
Peripheral immune tolerance serves as a core mechanism for maintaining immune homeostasis within the body. Regulatory T cells (Tregs) are the key cells responsible for performing this function. Through cell-contact-dependent mechanisms, secretion of inhibitory cytokines, and metabolic interference, Tregs actively suppress the activation of effector immune cells. In the local oral environment, the dynamic balance of the Treg/T helper cell 17 (Th17) axis is of crucial importance for maintaining oral health and represents a common immunopathological basis for various oral diseases. In periodontitis, the excessive activation of Th17 cells and the suppression of Treg function trigger chronic destructive inflammation and alveolar bone resorption. In mucosal diseases such as oral lichen planus and recurrent aphthous ulcers, the disorder of the Treg/Th17 axis results in pathological autoimmune attacks. In oral squamous cell carcinoma, the tumor microenvironment recruits Tregs to establish abnormal immune tolerance, which mediates immune escape and promotes tumor progression. Based on these findings, therapeutic strategies targeting the precise regulation of Treg function or the reshaping of the Treg/Th17 balance are emerging, including cytokine modulators, small molecule drugs, and engineered Treg cell therapies. However, current research continues to encounter bottlenecks, including the unclear mechanism of Tregs heterogeneity and the challenge of achieving both high targeting efficiency and safety in clinical translation. In the future, it is essential to integrate multi-omics technologies to comprehensively analyze the immune regulatory network within the oral microenvironment and develop precise delivery systems to facilitate clinical translation. This article conducts a systematic review of the mechanism of peripheral immune tolerance and the role of Tregs in oral diseases, thoroughly analyzes the characteristics of immune dysregulation in different diseases, and deliberates on the therapeutic strategies based on immune tolerance regulation and their clinical application prospects, aiming to provide a theoretical foundation for precise immune therapy of oral diseases.
[Mechanism Study of Aconitase 1 Promoting Hepatocellular Carcinoma Growth via the Itaconic Acid-Autophagy Axis].
Lü S, Yang Y, Jiang K … +3 more , Cheng C, Wang K, Li C
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021887 · Full text
OBJECTIVE: To investigate the expression level of aconitate decarboxylase 1 (ACOD1) in hepatocellular carcinoma (HCC) and its role in HCC cell growth, as well as the underlying mechanisms. METHODS: The expression of the...
OBJECTIVE: To investigate the expression level of aconitate decarboxylase 1 (ACOD1) in hepatocellular carcinoma (HCC) and its role in HCC cell growth, as well as the underlying mechanisms. METHODS: The expression of the 1 gene in HCC and its correlation with patient survival were analyzed using the GTEx and TCGA databases. ACOD1 protein expression in four paired HCC and adjacent non-tumor tissues was detected by Western blotting. After 1 knockdown in HepG2 and PLC/PRF/5 HCC cells, cell proliferation and death were assessed by MTT and LDH assays, respectively. Following 1 knockdown, 4-octyl itaconate (4-OI) was administered to observe changes in cell proliferation. Potential mechanisms were predicted using the cBioPortal database through KEGG enrichment analysis, and the expression of autophagy-related proteins LC3-Ⅱ and p62 was measured by Western blotting. After 1 knockdown, cells were treated with chloroquine (CQ) or supplemented with 4-OI to observe changes in autophagy marker proteins and cell proliferation. RESULTS: ACOD1 protein expression was significantly overexpressed in HCC tissues than in adjacent tissues (approximately 2-fold). Patients with ACOD1 overexpression had shorter overall survival ( < 0.05). Knocking down 1 in HepG2 and PLC/PRF/5 cells, which expressed high levels of ACOD1, inhibited cell proliferation (by approximately 20% and 30%, respectively; both < 0.05) and increased cell death (by approximately 30% in both cell lines; both < 0.05). 4-OI promoted cell growth in a concentration-dependent manner and reversed the decrease in cell viability caused by 1 knockdown, restoring it to 93% and 90% of control levels, respectively (both < 0.05). KEGG analysis suggested a potential association between ACOD1 and the autophagy pathway. 1 knockdown significantly increased LC3-Ⅱ levels (by approximately 7-fold and 10-fold, respectively; both < 0.05) and decreased p62 levels (by 40% and 30%, respectively; both < 0.05) in HepG2 and PLC/PRF/5 cells. CQ treatment increased the LC3-Ⅱ/LC3-Ⅰ ratio and p62 protein expression (both by approximately 2-fold; < 0.05). Combined knockdown further increased the LC3-Ⅱ/LC3-Ⅰ ratio (by approximately 10%; < 0.05), while p62 protein expression did not increase significantly ( > 0.05). Supplementation with 4-OI reversed the changes in LC3-Ⅱ and p62 protein expression induced by knockdown. Furthermore, CQ treatment partially rescued the decrease in viability of HepG2 and PLC/PRF/5 cells caused by knockdown (restoring viability to 75% and 80%, respectively; both < 0.05). CONCLUSION: ACOD1 is overexpressed in HCC and its' overexpression is associated with poor prognosis. ACOD1 promotes HCC cell growth by synthesizing itaconate, which inhibits cellular autophagy.
[Tibetan Medicine Classic Formula Srolo Bzhtang Granules Ameliorates Pulmonary Fibrosis via Dual Pathways of Nrf2/HO-1 and PI3K/AKT/mTOR Regulating Oxidative Stress].
DU J, Baimalazong, Dejibaizhen … +3 more , Basangdeji, Bianbaciren, Ye B
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021886 · Full text
OBJECTIVE: To investigate how Srolo Bzhtang (SBT), a classical Tibetan medicine formula, improves oxidative stress and ultimately alleviates pulmonary fibrosis in rats through the Nrf2/HO-1 and PI3K/AKT/mTOR pathways. ME...
OBJECTIVE: To investigate how Srolo Bzhtang (SBT), a classical Tibetan medicine formula, improves oxidative stress and ultimately alleviates pulmonary fibrosis in rats through the Nrf2/HO-1 and PI3K/AKT/mTOR pathways. METHODS: Seventy-two SD rats were randomly assigned to 12 sham surgery groups (Sham group, receiving equal volumes of normal saline) and 60 model groups (established by intratracheal instillation of bleomycin to induce pulmonary fibrosis). Twenty-four hours after modeling, the model groups were randomly divided into the model group (Model), the positive drug group (pirfenidone, 150 mg/kg), and low, medium, and high dose Soroxisol groups (SBT-L 0.5 g/kg, SBT-M 1.5 g/kg, SBT-H 4.5 g/kg), with 12 rats in each group. Each group received the drug by gavage once daily for 21 days.The Sham and Model groups received equal volumes of normal saline. HE and Masson staining were used to observe the pathological changes of pulmonary fibrosis in each group. ELISA was used to measure the levels of inflammatory factors (TNF-α, IL-8) and matrix metalloproteinases (MMP-2, MMP-9) in serum. The activities of malondialdehyde (MDA) and superoxide dismutase (SOD) in serum were also measured. The expression levels of Nrf2/HO-1 and proteins in the PI3K/AKT/mTOR signaling pathway in lung tissue were determined by Western blot. RESULTS: HE and Masson staining showed that pulmonary fibrosis was more severe in the Model group than in the Sham group, and each drug administration group could reverse this process to varying degrees. SBT inhibited the levels of inflammatory factors TNF-α and IL-8 (all < 0.05), and compared with the Model group, the levels of matrix metalloproteinases MMP-2 and MMP-9 were decreased (all < 0.05). Pathological section results showed that SBT improved lung tissue damage in pulmonary fibrosis rats to some extent. Compared with the Model group, MDA levels in the low-, medium-, and high-dose SBT groups decreased (all < 0.05), and SOD enzyme activity showed an increasing trend. Western blot results indicated that, compared with the Model group, the low-, medium-, and high-dose SBT groups activated the protein expression of Nrf2 and HO-1 (all < 0.05) and downregulated the expression levels of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR (all < 0.05). CONCLUSION: SBT affects the Nrf2/HO-1 and PI3K/AKT/mTOR signaling pathways, inhibits oxidative stress, and thereby delays the progression of pulmonary fibrosis in rats.
Nanoparticle Delivery Systems in Rheumatoid Arthritis: More Than Vehicles.
Zhang K, Guo Y, Zeng X … +4 more , Cai B, Zhang J, Li Y, Niu Q
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021885 · Full text
Rheumatoid arthritis (RA) is one of the most prevalent systemic autoimmune inflammatory diseases worldwide, causing chronic, progressively worsening arthritis that may ultimately lead to disability. Despite the availabil...
Rheumatoid arthritis (RA) is one of the most prevalent systemic autoimmune inflammatory diseases worldwide, causing chronic, progressively worsening arthritis that may ultimately lead to disability. Despite the availability of numerous therapeutic agents, limitations exhibit, including poor aqueous solubility, suboptimal stability, inadequate permeability, short half-lives, and multi-organ toxicity during long-term or high-dose administration. Nanoparticle-based drug delivery offers a robust strategy to mitigate these deficiencies while maximizing therapeutic efficacy through controlled-release mechanisms and rational administration route design. This review systematically summarizes recent advancements in nanoparticle drug delivery strategies for RA treatment from the perspective of three distinct mechanisms. It details the design rationales, therapeutic principles, and effects of various delivery systems, with particular emphasis on their interactions with the disease microenvironment and the entire body.
[ Axis Promotes Hepatocellular Carcinoma Progression and Therapeutic Resistance by Regulating Cell Cycle Proteins].
Zhou Y, Zhang H, Zhu Y … +4 more , Jiang P, Feng T, Wu Z, Chen J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021884 · Full text
OBJECTIVE: To investigate the expression pattern, prognostic significance, and underlying molecular mechanisms of mutY homolog () in hepatocellular carcinoma (HCC), and to evaluate its clinical potential as a novel bioma...
OBJECTIVE: To investigate the expression pattern, prognostic significance, and underlying molecular mechanisms of mutY homolog () in hepatocellular carcinoma (HCC), and to evaluate its clinical potential as a novel biomarker and therapeutic target. METHODS: The differential expression of between HCC and normal tissues was compared using the TCGA and GEO databases. Associations with clinicopathological parameters, mutation status, diagnostic efficacy of alpha-fetoprotein (AFP), and sorafenib resistance were analyzed. Prognostic impact was evaluated using the Kaplan-Meier method with the log-rank test, and univariate and multivariate Cox proportional hazards regression models were used to verify its independent prognostic value. Molecular mechanisms were explored through GO, KEGG, and GSEA enrichment analyses as well as protein-protein interaction (PPI) network construction. The correlations of with immune cell infiltration and the immunotherapeutic efficacy of immune checkpoint inhibitors were assessed using the CIBERSORT algorithm and the BEST database. Quantitative real-time PCR (qPCR) was performed to validate the expression differences of and its core interacting molecules between HCC and normal tissues. RESULTS: was significantly upregulated in HCC ( < 0.05), clinical sample tests have confirmed that it can serve as a biomarker for diagnosing HCC (area under the curve [AUC] = 0.824, 95% CI: 0.762-0.886, < 0.001), its diagnostic value remains high even in the HCC subgroup with low AFP expression (GSE25097, AUC = 0.716, < 0.001; GSE63898, AUC = 0.624, < 0.001). High expression correlated with sorafenib resistance ( < 0.05) and was an independent risk factor for poor overall survival (hazard ratio [HR] = 1.92, < 0.05). Mechanistically, MUTYH was positively associated with apurinic/apyrimidinic endonuclease 1 (APEX1) ( = 0.83, < 0.05), potentially facilitating G/S transition by modulating cyclin-dependent kinase 4, cyclin-dependent kinase 7, and cyclin E2. Immune analysis identified as a predictor for anti-PD-1/PD-L1 response (IMvigor210 AUC = 0.637; Cho2020 AUC = 0.782), though no association was found with anti-CTLA-4 therapy. CONCLUSION: is significantly overexpressed in HCC and may promote HCC progression by regulating and key cell cycle molecules. Compared with the conventional marker AFP, demonstrates superior diagnostic and prognostic evaluation efficacy and is associated with anti-PD-1/PD-L1 therapeutic response and sorafenib resistance. Overall, has potential as a novel biomarker and therapeutic target for HCC.
[The Influence of Hepatitis B Virus on Sex Hormone Levels and Quality of Life in Female Hepatitis B Patients With Different Alanine Aminotransferase States].
Gao P, Zhou W, Li J … +1 more , Yao L
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021883 · Full text
OBJECTIVE: This study examines the effects of hepatitis B virus on sex hormone levels, menopause, sleep, depression, anxiety, and quality of life in female hepatitis B patients under different alanine aminotransferase (A...
OBJECTIVE: This study examines the effects of hepatitis B virus on sex hormone levels, menopause, sleep, depression, anxiety, and quality of life in female hepatitis B patients under different alanine aminotransferase (ALT) conditions. METHODS: A total of 306 female patients with chronic hepatitis B, exhibiting different viral loads and varying ALT statuses, were randomly selected for stratified sampling. The effects of hepatitis B virus on sex hormone levels, menopause, sleep, depression, anxiety, and quality of life were compared and analyzed across different ALT statuses. Among these, 110 female patients with chronic hepatitis B and ALT levels ≥ 2 times the upper limit of normal received antiviral treatment and were followed for 24 weeks. Changes in the above indicators before and after antiviral treatment were compared and analyzed. RESULTS: In the ALT-normal group and the group with ALT levels ≤ 2 times the upper limit of normal, there was no significant difference in the effects of different hepatitis B virus viral loads on sex hormone levels, menopause, sleep, depression, anxiety, or quality of life in female patients with chronic hepatitis B. However, in the group with ALT ≥ 2 times the upper limit of normal, as the viral load increased (from the low replication group to the high replication group), the estradiol concentration decreased from (84.20 ± 7.78) pg/mL to (64.60 ± 9.18) pg/mL, testosterone increased from (0.33 ± 0.02) ng/mL to (0.45 ± 0.04) ng/mL, follicle-stimulating hormone increased from (47.82 ± 7.62) mIU/mL to (59.68 ± 7.19) mIU/mL, the menopause rate increased from 55.56% to 86.11%, the Pittsburgh Sleep Quality Index increased from 11.02 ± 0.52 to 15.93 ± 0.71, the Self-Rating Anxiety Scale score increased from 46.06 ± 4.92 to 64.66 ± 6.18, the Self-Rating Depression Scale score increased from 44.14 ± 5.47 to 67.08 ± 4.57, and all dimensions of quality of life significantly decreased, with all differences being statistically significant ( < 0.05). After antiviral treatment in 110 female patients with ALT ≥ 2 times the upper limit of normal, estradiol significantly increased, testosterone and follicle-stimulating hormone significantly decreased, sleep indicators and depression and anxiety scores significantly decreased, and all quality of life indicators significantly increased (all < 0.05). CONCLUSION: The impact of hepatitis B virus on female patients varies under different ALT conditions. For patients with ALT levels at or above twice the upper limit of normal, actively initiating antiviral treatment can significantly improve sex hormone levels and various physiological and psychological indicators in female patients, thereby enhancing their quality of life.
[Detection and Distribution Characteristics of Antibiotic Resistance Genes in Companion Animals].
Wang R, An L, Pei X … +1 more , Chen J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021882 · Full text
OBJECTIVE: To explore the distribution characteristics of antimicrobial resistance genes (ARGs) in companion animals and accumulate scientific data for preventing the transmission of ARGs between companion animals and hu...
OBJECTIVE: To explore the distribution characteristics of antimicrobial resistance genes (ARGs) in companion animals and accumulate scientific data for preventing the transmission of ARGs between companion animals and humans. METHODS: From December 2023 to October 2024, fecal or rectal swab samples were collected from companion animals at a large pet hospital in Chengdu. PCR and qPCR were used to detect 9 ARGs types (including 20 ARGs). The distribution characteristics of ARGs in companion animals with different species, gender, age, clinical status and the mobile genetic element carrying status were analyzed based on the data of ARGs quantities, detection rates and relative abundance. RESULTS: A total of 136 samples were collected. The median (interquartile range) of ARGs detected per animal was 6.5 (5, 9). Tetracycline and aminoglycoside resistance genes had higher detection rates, with and having the highest detection rates (both at 90.44%), and relative abundance > 10. This was followed by (67.65%) and (58.82%), with relative abundance > 10. Female animals had higher relative abundance of , , and than male animals. The detection rate of in animals aged 0-2 months was higher than that in animals aged 12 months and above ( = 0.007). The detection rates of macrolide resistance genes and in animals aged 3-5 months were higher than those in animals aged 12 months and above ( < 0.001). Animals carrying had higher ARGs detection quantities, detection rates and relative abundance of multiple ARGs than those not carrying , with all differences being statistically significant. CONCLUSION: Companion animals commonly carry ARGs, with tetracycline and aminoglycoside resistance genes having high detection rates. Age and the carriage of are important factors influencing the distribution of ARGs in companion animals.
[A Randomized Controlled Clinical Trial of Acupuncture at the Sphenopalatine Ganglion for the Treatment of Allergic Rhinitis].
Huang J, Zhong B, Wang F … +5 more , Zhang Y, Qiu Y, Wei X, Deng J, Song H
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021881 · Full text
OBJECTIVE: To evaluate the clinical efficacy and safety of acupuncture at the sphenopalatine ganglion (SPG) for the treatment of allergic rhinitis (AR). METHODS: A randomized controlled trial design was used. A total of...
OBJECTIVE: To evaluate the clinical efficacy and safety of acupuncture at the sphenopalatine ganglion (SPG) for the treatment of allergic rhinitis (AR). METHODS: A randomized controlled trial design was used. A total of 120 patients with AR were randomly assigned in a 1∶1 ratio to either the experimental group (acupuncture on the SPG, twice a week for 2 weeks) or the control group (oral lupatadine fumarate tablets, 10 mg daily for 2 weeks). The primary outcome measures were nasal symptom and sign scores at 1 and 2 weeks after treatment. Secondary outcome measures included the total non-nasal symptom score (TNNSS) and the rhinoconjunctivitis quality of life questionnaire (RQLQ) score. RESULTS: Four cases were excluded from both the experimental group and the control group. Ultimately, 56 cases in each group completed the study. After two weeks of treatment, the total effective rates were 82.1% for the experimental group and 87.5% for the control group, with no statistically significant difference. At each time point after treatment, the symptom scores, sign scores, TNNSS, and RQLQ scores in both groups decreased compared to baseline, and these differences were statistically significant ( < 0.001). Generalized estimating equation (GEE) analysis showed that the improvement trends for most indicators were consistent between the two groups, but there was a "group × time" interaction, indicating that improvement in nasal congestion symptoms in the experimental group was greater than in the control group during the first week of treatment (experimental group: 2.0 [1.0, 2.0] vs. control group: 2.0 [1.0, 2.0], interaction = 0.023). Forest plot analysis further showed that the standardized mean difference (SMD) for nasal congestion symptoms at one week of treatment was －0.420 (95% confidence interval: －0.795, －0.046), and the 95% confidence intervals for all indicators' SMD at two weeks crossed zero. Regarding safety, there was no statistically significant difference in the incidence of adverse events between the experimental and control groups. CONCLUSION: Acupuncture on the SPG and oral administration of lupatadine fumarate can both effectively alleviate symptoms and improve the quality of life for patients with AR. The overall short-term efficacy is comparable. However, acupuncture on the SPG is more effective in relieving nasal congestion and has a good safety profile.
[Construction and Efficacy Evaluation of a Highly Efficient Diagnostic Model for Neurosyphilis Driven by the Cerebrospinal Fluid/Serum Free Light Chain Index].
Deng J, Wu S, Ye F … +3 more , Huang X, Li D, Liu J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021880 · Full text
OBJECTIVE: To determine free light chains (FLC) in cerebrospinal fluid (CSF) and serum, calculate the FLC index, and establish a model to evaluate its application value in diagnosing neurosyphilis. METHODS: This study in...
OBJECTIVE: To determine free light chains (FLC) in cerebrospinal fluid (CSF) and serum, calculate the FLC index, and establish a model to evaluate its application value in diagnosing neurosyphilis. METHODS: This study included 87 syphilis patients, comprising 48 neurosyphilis patients (NS group) and 39 non-neurosyphilis patients (n-NS group). CSF and serum samples were collected from all patients to measure kappa free light chains (FLC-κ) and lambda free light chains (FLC-λ). The FLC index, FLC-κ index, and FLC-λ index were calculated. Receiver operating characteristic (ROC) curves were used to evaluate these indices. A neurosyphilis diagnostic model based on the FLC index was established and its visualization was verified. RESULTS: Statistically significant differences were observed between the two groups in serological syphilis screening, CSF nucleated cell count, CSF microprotein, CSF IgG, IgG synthesis rate, IgG index, and albumin quotient ( < 0.05). The areas under the ROC curves (AUC) for the FLC-κ index, FLC-λ index, and albumin quotient were 0.998 (95% CI: 0.990-1.000), 0.988 (95% CI: 0.961-1.000), and 0.635 (95% CI: 0.496-0.751), respectively. In the FLC-κ index model, the FLC-κ index ( < 0.05) significantly affected the diagnosis of neurosyphilis, while in the FLC-λ index model, only the FLC-λ index ( < 0.05) had a significant effect. The training ROC curve AUC for the diagnosis model based on the FLC-κ index was greater than 0.950. Its calibration curve showed good predictive performance when the predicted probability was close to 80%, but poor performance when the predicted probability was 40%-60%. The AUC for the diagnosis model based on the FLC-λ index was 0.926, with good predictive performance when the predicted probability was close to 85%, and poor performance when the predicted probability was 40%-70%. CONCLUSION: FLC-κ and FLC-λ have high diagnostic performance for neurosyphilis, and the FLC index has certain application value in the diagnosis of neurosyphilis.
[ROMO1 Promotes the Repair of Radiation-induced Skin Injury by Regulating Mitochondrial Function and Keratinocyte Proliferation].
Yang T, Tu W, Tang X … +6 more , DU M, Maheliya A, Yu Z, Qu S, Bai H, Zhang S
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021879 · Full text
OBJECTIVE: To investigate the molecular mechanisms of early mitochondrial stress in keratinocytes induced by ionizing radiation (IR), focusing on the key role of ROMO1 in radiation-induced skin injury (RISI). METHODS: Ha...
OBJECTIVE: To investigate the molecular mechanisms of early mitochondrial stress in keratinocytes induced by ionizing radiation (IR), focusing on the key role of ROMO1 in radiation-induced skin injury (RISI). METHODS: HaCaT keratinocytes were used to assess cellular status, mitochondrial dysfunction and oxidative stress at various time points after different irradiation doses. Mitochondrial proteomics at 24 hours post-20 Gy irradiation identified ROMO1 as the protein with the greatest reduction in expression. -overexpressing HaCaT cells and skin-specific knockout (-sKO) mice were established to investigate its functional mechanisms. ROMO1 expression and localization were analyzed by Western blot and immunofluorescence; flow cytometry measured ROS, mtROS, and mitochondrial membrane potential in -overexpressing cells, while CCK-8, LDH release, and colony formation assays evaluated viability and proliferation. The role of in the progression and repair of radiation-induced skin injury was further examined in -sKO mice. RESULTS: At 24 hours post-20 Gy irradiation, HaCaT cells exhibited inhibited proliferation ( < 0.05) and mitochondrial dysfunction ( < 0.0001). Mitochondrial proteomics identified 43 differentially expressed proteins (26 upregulated, 17 downregulated), with enrichment analysis indicating their involvement in oxidative phosphorylation, mitochondrial assembly and stress response. Among these, reactive oxygen species modulator 1 (ROMO1) was identified as the most significantly downregulated key protein in the early radiation response ( < 0.01, FDR = 0.03). , ROMO1 overexpression increased mtROS levels and mitochondrial membrane potential ( < 0.0001) but suppressed cell viability and promoted reproductive death ( < 0.05) after radiation. , -sKO mice showed accelerated wound healing, with a reduction in skin injury score by approximately 1 point, along with enhanced tissue regeneration at 25 days post-irradiation compared to control mice. CONCLUSION: The mitochondrial protein ROMO1 is a key regulator of the early keratinocyte stress response to radiation. It impedes the repair of radiation-induced skin injury by suppressing cell proliferation via modulation of mitochondrial function. Targeted inhibition of ROMO1 may represent a novel therapeutic strategy to promote healing and prevent chronic progression of radiation-induced skin injury.
[Protein Expression Levels of the DCAF4-MEN1-hTERT Axis in Patients With Colon Adenocarcinoma and Their Relationship With Clinicopathological Characteristics and Prognosis].
Zhu S, Chen S, Zheng Q … +1 more , Liu H
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021878 · Full text
OBJECTIVE: To explore the relationship between the expression levels of DNA damage binding protein 1 and cullin 4-related factor 4 (DCAF4) complex, which degrades the tumor suppressor menin 1 (MEN1)-human telomerase reve...
OBJECTIVE: To explore the relationship between the expression levels of DNA damage binding protein 1 and cullin 4-related factor 4 (DCAF4) complex, which degrades the tumor suppressor menin 1 (MEN1)-human telomerase reverse transcriptase (hTERT) axis protein, and the clinicopathological characteristics and prognosis of patients with colon adenocarcinoma. METHODS: Ninety-four patients with colon adenocarcinoma were included. The expression of DCAF4, MEN1, and hTERT in cancer tissues and adjacent tissues was detected by immunohistochemistry. The expression levels of DCAF4, MEN1, and hTERT in patients with different pathological grades and T stages were compared. Spearman rank correlation was used for correlation analysis, and survival analysis was conducted to investigate the impact of DCAF4, MEN1, and hTERT expression levels on the overall survival of patients with colon adenocarcinoma. RESULTS: The expression levels of DCAF4 and MEN1 proteins in cancer tissues of colon adenocarcinoma patients (10.83 ± 2.89; 9.71 ± 3.57) were higher than those in adjacent tissues (2.39 ± 1.57; 4.92 ± 2.71, < 0.001). The expression level of DCAF4 in patients with grade Ⅱ colon adenocarcinoma (14.16 ± 4.67) was lower than in patients with grade Ⅲ (20.79 ± 5.06, < 0.01), while the expression of MEN1 (10.74 ± 3.06) was higher than in patients with grade Ⅲ (8.07 ± 2.88, < 0.001), while there was no statistically significant difference in the protein expression scores of MEN1 and hTERT between the two groups. Compared with colon adenocarcinoma patients carrying the wild-type gene (5.47 ± 1.81), those with the mutant-type gene had a higher expression score of DCAF4 protein (9.30 ± 0.42, < 0.001). The expression of MEN1 was correlated with the positive rate of PD1 (Spearman = 0.219, = 0.034). Compared with patients in stage T2 (3.93 ± 2.47), patients in stage T3 had a higher expression score of hTERT protein (6.25 ± 3.04, < 0.05). Survival analysis showed that the expressions of MEN1, hTERT, and DCAF4 were not significantly associated with overall survival. CONCLUSION: High expression of DCAF4 and hTERT, as well as low expression of MEN1, is associated with unfavorable clinical and pathological features but has no significant relationship with prognosis.
[Screening of High-quality Spermatozoa Using Transwell Membrane Pores and Assessment of Their Performance].
Li Y, Liu Y, Zhang S … +5 more , Huang X, Weng Z, Zhou S, Liu Z, Li R
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021877 · Full text
OBJECTIVE: The feasibility of using Transwell, based on the molecular sieve effect of its membrane pores, to select high-quality human sperm was explored. METHODS: A total of 162 fresh semen samples were collected from t...
OBJECTIVE: The feasibility of using Transwell, based on the molecular sieve effect of its membrane pores, to select high-quality human sperm was explored. METHODS: A total of 162 fresh semen samples were collected from the Department of Andrology at the First Affiliated Hospital of Guangzhou University of Chinese Medicine between November 2023 and May 2024. Differences in sperm selection using 5 μm and 8 μm pore Transwell filters were analyzed, and microscopic structural differences were observed by transmission electron microscopy (TEM). Changes in semen parameters after 30, 60, and 80 minutes of Transwell sperm selection were compared. Differences in semen parameters after optimization of sperm of varying qualities by Transwell, density gradient centrifugation (DGC), and swim-up (SU) methods were also compared. In this study, the percentage of progressive motility (PR) of sperm was set as the primary outcome measure, while sperm motility, normal sperm morphology rate (NSMR), DNA fragmentation index (DFI), sperm concentration, and recovery rate were set as secondary outcome measures. RESULTS: When the aperture is 5 μm and the preferred time is 60 minutes, the Transwell method yields better results for sperm selection. In the normal semen group, compared with the DGC method, the median percentage of PR in sperm after Transwell selection increased from 77.50% to 92.05% ( < 0.05), the median NSMR increased from 9.00% to 13.25% ( < 0.01), and the median DFI decreased from 2.16% to 0.25% ( < 0.01). Compared with the SU method, the median sperm concentration after Transwell selection increased from 4.70 × 10 mL to 14.15 × 10 mL ( < 0.01), the median DFI decreased from 0.47% to 0.25% ( < 0.01), and the median recovery rate increased from 2.50% to 6.48% ( < 0.01). but there were no statistically significant differences in sperm motility, PR percentage, or NSMR. In the abnormal semen group, compared with the DGC method, the median PR percentage of sperm after Transwell selection increased from 37.20% to 88.75% ( < 0.01), the median sperm motility increased from 53.00% to 95.00%, and the median DFI decreased from 12.49% to 0.92% ( < 0.01). Compared with the SU method, the median PR percentage of sperm after Transwell selection increased from 86.30% to 88.75% ( < 0.05), and the median DFI decreased from 1.63% to 0.92% ( < 0.05). CONCLUSION: The Transwell preferred method demonstrates excellent performance in obtaining sufficient sperm with high viability, good morphology, and good DNA integrity. This makes it a promising, simple, and practical method for selecting high-quality sperm in assisted reproductive technologies.
[Establish Standardized Pathological and Genetic Examinations to Reduce the Incidence of Stillbirth].
Zhou F, Yu H, Wang W … +2 more , Wan L, Wang X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021876 · Full text
Stillbirths represent the most severe adverse fetal outcomes and constitute a significant public health issue. China experiences considerable regional disparities in stillbirth rates, with the total number of stillbirths...
Stillbirths represent the most severe adverse fetal outcomes and constitute a significant public health issue. China experiences considerable regional disparities in stillbirth rates, with the total number of stillbirths ranking fourth globally. Major challenges remain in improving the standardization of stillbirth management and reducing stillbirth incidence. This article presents a comprehensive review of the international classification systems for stillbirth causes, with particular emphasis on key components such as placental and fetal pathological examinations and genetic testing strategies. It highlights the critical role of pathological and genetic investigations in identifying the etiology and guiding the clinical management of stillbirth. Furthermore, the article advocates for the establishment of a tiered management framework for stillbirth etiology tailored to China's national context, promotes the standardization and clinical implementation of pathological and genetic testing, enhances understanding of the underlying pathophysiological mechanisms and causes of stillbirth, and thereby enables the development of preventive strategies at the causal level to effectively reduce stillbirth rates.
[Advances in the Application of Artificial Intelligence in Clinical Microbiological Testing].
Li H, Song Z, Zhao Y … +1 more , Li M
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021875 · Full text
Traditional microbiological detection methods have inherent limitations in detection speed, sensitivity, and specificity, making them increasingly unable to meet growing clinical demands. In recent years, artificial inte...
Traditional microbiological detection methods have inherent limitations in detection speed, sensitivity, and specificity, making them increasingly unable to meet growing clinical demands. In recent years, artificial intelligence (AI) has been rapidly integrated into clinical microbiological testing, with numerous studies demonstrating its significant potential to enhance pathogen identification, predict antimicrobial susceptibility testing, and advance laboratory automation. This article systematically reviews classical AI algorithms and their latest advancements in this field. For visual data applications, deep learning-based models are used to automatically analyze microscopy images or colony morphology, significantly improving recognition efficiency and diagnostic accuracy. For non-visual data, AI has achieved breakthroughs in analyzing multi-omics data such as genomics, transcriptomics, and metagenomics, and is widely used for rapid pathogen identification and prediction of antimicrobial resistance. Despite its promising prospects, the application of AI in clinical microbiological testing remains in the early stages of transitioning from scientific research to clinical practice. This paper further discusses the key challenges and opportunities encountered during this technological translation, aiming to help clinical professionals comprehensively understand the current status, future trends, and potential impact of AI in this field, thereby promoting its development into reliable and scalable routine diagnostic methods.
[Analysis of the Advantages and Clinical Validation of Real-Time Fluorescence Nucleic Acid Isothermal Amplification Detection Technology in Detecting Low Viral Load Samples From HBV-Infected Patients].
Huang Q, Xie R, Lan Y … +3 more , Xun Z, Ou Q, Liu C
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Mar · PMID 42021874 · Full text
OBJECTIVE: This paper examines the advantages and clinical application value of simultaneous amplification and testing (SAT) real-time fluorescent nucleic acid isothermal amplification detection technology for detecting...
OBJECTIVE: This paper examines the advantages and clinical application value of simultaneous amplification and testing (SAT) real-time fluorescent nucleic acid isothermal amplification detection technology for detecting low viral load samples in individuals infected with hepatitis B virus (HBV). METHODS: Using reverse transcription real-time fluorescence quantitative PCR (RT-qPCR) as the reference, the detection performance of real-time fluorescence nucleic acid isothermal amplification detection technology (SAT) was evaluated, including linear range, precision, and detection limit. Both methods were used to detect samples, which were the national standard substances of hepatitis B virus ribonucleic acid (HBV RNA). A total of 170 patients with chronic HBV infection were included for methodological comparison. They were divided into a high-level group (serum HBV DNA > 100 IU/mL, = 111) and a low-level group (serum HBV DNA ≤ 100 IU/mL, = 59) based on serum HBV DNA levels. The correlation and consistency of the test results from the two methods were evaluated. Additionally, the distribution characteristics of HBV RNA and its correlation with HBV markers were analyzed using data from 1006 patients with chronic HBV infection. RESULTS: Compared with the RT-qPCR method for detecting HBV RNA, the SAT method demonstrated a wider linear range (10-10 copies/mL vs. 10-10 copies/mL), higher precision for low-concentration samples (intra-assay coefficient of variation: 4.23% vs. 12.82%), and a lower detection limit (50 copies/mL vs. 500 copies/mL). In clinical sample testing, the overall detection rate of the SAT method was higher than that of the RT-qPCR method (72.35% vs. 57.64%, < 0.01), and the detection rate of the SAT method was also higher in the HBV DNA low-level group (50.85% vs. 28.81%, = 0.007). Large-sample analysis showed that among patients with HBV DNA < 20 IU/mL, 40.4% still had detectable HBV RNA, and the positive rate for HBsAg ≥ 100 IU/mL was 55.5%. Correlation analysis indicated that HBV RNA was moderately positively correlated with HBsAg ( = 0.506) and HBeAg ( = 0.454), and weakly negatively correlated with ALT ( = －0.098) and AST ( = －0.082) (all < 0.05). CONCLUSION: The SAT method offers higher sensitivity and stability in detecting low-level HBV RNA. HBV RNA can serve as a serological marker for evaluating viral transcription activity and has clinical application value in managing patients who are HBV DNA-negative or have low HBV DNA levels.
[Exercise Load Classification and Perceptual Difference Awareness Training Based on Exercise Load Entropy Theory in University Students].
Li X, Yang P, Wang L … +4 more , Han H, Bai P, Li J, Xiao X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834988 · Full text
OBJECTIVE: Based on exercise load entropy measurement theory, this study aimed to investigate patterns of change in exercise load classification, perceptual difference awareness, and Weber fraction among university stude...
OBJECTIVE: Based on exercise load entropy measurement theory, this study aimed to investigate patterns of change in exercise load classification, perceptual difference awareness, and Weber fraction among university students across different exercise load levels, thereby providing theoretical support for personalized exercise load assessment. METHODS: From January to April 2025, 138 college students completed six incrementally increasing load tests on a cycle ergometer. Based on self-perceived load staging and perceptual discrimination, discrimination thresholds and Weber fractions were calculated for each load level. Data were analyzed using univariate analysis, correlation analysis, and multiple linear regression analysis. RESULTS: With increasing exercise load levels, differential perception showed an overall downward trend, but the difference was not statistically significant ( = 0.156). The Weber fraction decreased progressively with increasing load level, from 0.49 at load level 2 to 0.11 at load level 6 ( = 0.034). Multiple linear regression analysis indicated that for each one-level increase in exercise load, the Weber fraction decreased by an average of 0.07 (95% CI: －0.12 to －0.02, = 0.009). In addition, males had a lower Weber fraction than females (95% CI: －0.12 to －0.06, < 0.001). CONCLUSION: Among college students, the perception of exercise load changes nonlinearly as the load level increases, and the Weber fraction decreases as the load intensifies.
[Electroacupuncture Alleviates Acute Kidney Injury in Septic Mice By Inhibiting the P2RX7/NLRP3 Signaling Pathway].
Wang M, Zhao X, Hou S … +2 more , Xiao X, Yin H
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834987 · Full text
OBJECTIVE: To investigate the protective effects and mechanisms of electroacupuncture on the P2X7 purinergic receptor/NLRP3 signaling pathway in sepsis-associated acute kidney injury (SA-AKI). METHODS: Healthy male C57BL...
OBJECTIVE: To investigate the protective effects and mechanisms of electroacupuncture on the P2X7 purinergic receptor/NLRP3 signaling pathway in sepsis-associated acute kidney injury (SA-AKI). METHODS: Healthy male C57BL/6J mice, 6-8 weeks old, were randomly assigned to the following groups: control, model, electroacupuncture, P2RX7 antagonist + model, and P2RX7 antagonist + model + electroacupuncture. The SA-AKI model was established by intraperitoneal injection of lipopolysaccharide (LPS). The P2RX7 antagonist A438079 was administered intraperitoneally 1 hour before LPS injection. Electroacupuncture (10 Hz, 0.5 mA, 30 min) was performed 1.5 hours after LPS injection. Mouse survival rates were assessed within 24 hours after modeling. Serum creatinine (Scr) levels were measured by blood biochemistry, IL-1β and IL-18 levels in serum and kidney tissues were measured with ELISA. Renal histopathological changes were observed by HE staining. Real-time fluorescent PCR and immunofluorescence assays were used to assess renal P2RX7 and NLRP3 expression levels. RESULTS: The 24-hour survival rate in the electroacupuncture group was 45%, a 15% improvement over the model group. Electroacupuncture treatment reduced renal histopathological damage, lowered Scr levels in SA-AKI ( < 0.05), and decreased serum inflammatory mediators IL-1β and IL-18 (both < 0.0001). Electroacupuncture also reduced renal tissue expression levels of IL-1β ( < 0.0001), IL-18 ( < 0.001), P2RX7, and NLRP3 (both < 0.05). CONCLUSION: The mechanism by which electroacupuncture ameliorates SA-AKI may involve inhibition of the P2RX7/NLRP3 signaling pathway and attenuation of systemic inflammatory responses.
[Role of Galectin-9 and Sonic Hedgehog Expressions in the Early Diagnosis and Prognosis of Colorectal Cancer].
He Q, Liu X, Lin T … +1 more , Dong X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834986 · Full text
OBJECTIVE: To investigate the association between the expression levels of galectin-9 and sonic hedgehog () and the risk of occurrence and prognosis in patients with colorectal cancer (CRC). METHODS: A total of 210 patie...
OBJECTIVE: To investigate the association between the expression levels of galectin-9 and sonic hedgehog () and the risk of occurrence and prognosis in patients with colorectal cancer (CRC). METHODS: A total of 210 patients with CRC treated at Qingdao Traditional Chinese Medicine Hospital between May 2020 and May 2022 were retrospectively included. The expressions of galectin-9 and in tumor tissues were measured. Logistic regression was used to analyze the relationship between galectin-9/ expression and CRC risk. Receiver operating characteristic (ROC) curves were plotted to evaluate the predictive value of galectin-9 and for CRC risk and prognosis. RESULTS: The expression levels of galectin-9 and differed significantly between cancer tissues and adjacent normal tissues (<0.05). ROC analysis showed that the AUCs for galectin-9 and in evaluating CRC risk were 0.809 (95% CI: 0.751-0.868) and 0.865 (95% CI: 0.817-0.912), respectively. Galectin-9 expression was lower in patients with T stage, N stage, M stage, or poor differentiation (<0.05), whereas SHH expression was higher in these patients(<0.05). Galectin-9 expression was negatively correlated with expression and TNM stage (=－0.184 and －0.362, respectively; <0.001). expression was positively correlated with TNM stage ( = 0.407, < 0.001). Significant differences in galectin-9 and expression were observed between patients with poor prognosis and those with good prognosis (< 0.05). Logistic regression analysis indicated that T stage (OR= 4.609, 95% CI: 1.461-14.535), poor differentiation (OR=3.337, 95% CI: 1.297-8.582), and high SHH expression (OR= 2.067, 95% CI: 1.162-3.678) were risk factors for poor prognosis (< 0.05), while high galectin-9 expression (OR = 0.652, 95% CI: 0.437-0.975) was a protective factor (< 0.05). The AUCs of galectin-9 and for evaluating CRC prognosis were 0.769 (95% CI: 0.550-0.798) and 0.734 (95% CI: 0.603-0.866), respectively. CONCLUSION: Galectin-9 and SHH are lowly and highly expressed, respectively, in CRC. Their expression levels are associated with tumor differentiation, invasion, and metastasis, and hold predictive value for the risk and prognosis of CRC patients.
[Efficacy of Norvancomycin in the Treatment of Acute Hematogenous Osteomyelitis in Children and Its Effect on Inflammatory Indicators].
Zhang X, Zhang N, Pei Y … +1 more , Zhao Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834985 · Full text
OBJECTIVE: To evaluate the clinical efficacy of norvancomycin in pediatric patients with infection-related acute hematogenous osteomyelitis (AHO) and its impact on inflammatory markers. METHODS: This study retrospectivel...
OBJECTIVE: To evaluate the clinical efficacy of norvancomycin in pediatric patients with infection-related acute hematogenous osteomyelitis (AHO) and its impact on inflammatory markers. METHODS: This study retrospectively analyzed children with infection-related AHO admitted to Hebei Children's Hospital from January 2016 to December 2024. Patients were divided into the vancomycin group (Group A, = 103) and the norvancomycin group (Group B, = 107) based on medication regimens. Baseline characteristics, including age, gender, weight, and lesion location, were adjusted for confounding factors using propensity score matching and multivariate regression analysis. Clinical efficacy and changes in inflammatory markers were compared between groups, including white blood cell (WBC) count, neutrophil (NE) count, C-reactive protein (CRP), and serum amyloid A (SAA) levels. RESULTS: There was no statistically significant difference in clinical cure rates between the two groups ( > 0.05). At 1 and 3 weeks post-treatment, CRP and SAA levels showed statistically significant time effects ( = 503.00 and 703.400, respectively, < 0.05). Both WBC and NE levels showed statistically significant time effects and between-group effects ( = 259.100 and 203.500, respectively; = 8.403 and 6.884, respectively; < 0.05), WBC, NE, CRP, and SAA levels gradually declined at both 1 week and 3 weeks post-treatment ( < 0.05). Group A had higher WBC levels than Group B at 1 week post-treatment ( < 0.05) and higher NE levels at 3 weeks post-treatment ( < 0.05). The time required for WBC and NE to return to normal levels was longer in Group A than in Group B ( = 2.051, 2.001, < 0.05), while the difference in recovery time for CRP and SAA between the two groups was not statistically significant ( > 0.05). Group A had a longer duration of fever resolution than Group B ( = 2.010, < 0.05). No statistically significant intergroup difference was observed in the time to resolution of clinical symptoms such as pain and swelling ( > 0.05). The overall incidence of adverse reactions was 14.56% in Group A and 7.48% in Group B, with no statistically significant intergroup difference ( > 0.05). The per-patient treatment cost and cost-effectiveness ratio were higher in Group A than in Group B ( = 14.385, < 0.05). CONCLUSION: Norexvancomycin achieved clinical cure rates comparable to those of vancomycin in treating infection-associated AHO. Furthermore, it demonstrated advantages in accelerating the recovery of WBC and NE counts and the resolution of fever. These clinical benefits were coupled with lower per-patient costs and more favorable cost-effectiveness ratios compared to vancomycin.
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