Searches
/
Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
Sun
200 papers
[Development and Validation of a Risk Prediction Model for Secondary Pulmonary Infarction in Elderly Patients With Acute Pulmonary Embolism].
Chen S, Weng M, Lü Y … +2 more , Jiang Y, Pang Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834984 · Full text
OBJECTIVE: To investigate the risk factors for secondary pulmonary infarction (PI) in elderly patients with acute pulmonary embolism (PE) and to construct a risk prediction model based on these factors. METHODS: This stu...
OBJECTIVE: To investigate the risk factors for secondary pulmonary infarction (PI) in elderly patients with acute pulmonary embolism (PE) and to construct a risk prediction model based on these factors. METHODS: This study enrolled 147 elderly PE patients hospitalized at our institution from July 2018 to September 2022. Patients were divided into a secondary PI group ( = 44) and a non-secondary PI group ( = 103) based on the occurrence of secondary PI. Stepwise regression analysis was used to identify risk factors for secondary PI, and a risk prediction model was constructed using R software. The model was validated with 63 elderly PE patients admitted between September 2022 and December 2023. Stepwise regression analysis identified alcohol consumption (odds ratio [OR] = 8.586, 95% CI: 2.430-30.361), chronic bronchitis (OR = 9.831, 95% CI: 2.701-35.782), emphysema (OR = 6.990, 95% CI: 1.987-24.582), coronary heart disease (OR = 15.603, 95% CI: 3.470-41.144), diabetes (OR = 11.955, 95% CI: 1.097-130.860), and D-dimer (OR = 1.021, 95% CI: 1.002-1.037) as independent risk factors for secondary PI in elderly PE patients ( < 0.05). Receiver operating characteristic (ROC) curve analysis showed an area under the curve (AUC) of 0.936 (95% CI: 0.901-0.976) for the modeling group and 0.917 (95% CI: 0.852-0.990) for the validation group. Calibration curve results indicated that the model demonstrated high accuracy in both the modeling and validation cohorts. Clinical decision curve analysis showed the model has high clinical utility. CONCLUSION: Elderly PE patients with alcohol consumption, chronic bronchitis, coronary heart disease, emphysema, diabetes, or elevated D-dimer levels have a higher risk of secondary PI. The predictive model demonstrates high discriminatory power and accuracy, aiding clinical assessment of secondary PI in PE patients.
[Research Progress on Microbiome-Driven Mechanisms and Intervention Strategies for Oral Malodor].
Shang X, Wang Z, Ge S
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834983 · Full text
Oral malodor, a common oral symptom, is primarily caused by the metabolic activities of oral microorganisms. The characteristic odors mainly originate from volatile sulfur compounds produced and released by oral bacteria...
Oral malodor, a common oral symptom, is primarily caused by the metabolic activities of oral microorganisms. The characteristic odors mainly originate from volatile sulfur compounds produced and released by oral bacteria (such as and ) through specific enzymatic systems that break down sulfur-containing amino acids in saliva, gingival crevicular fluid, and food debris. Research indicates that various factors, including periodontitis, fungal infections, smoking, and obesity, can worsen oral malodor by altering the oral microenvironment. For prevention and treatment, novel interventions such as plant extracts, antimicrobial peptides, and probiotics demonstrate superior microbiological safety compared to traditional antimicrobial drugs. These approaches specifically inhibit pathogenic bacteria, disrupt biofilm structures, and regulate oral microbial balance. This review summarizes relevant research advances to provide new theoretical foundations and practical directions for the precise prevention and treatment of oral malodor.
[Clinical Progress and Prospects of mRNA Tumor Drugs].
Chen J, Tang X, Liu S … +3 more , Guo X, Zhao C, Song X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834982 · Full text
mRNA-targeted tumor drugs, featuring various types and distinct antitumor mechanisms of action, have demonstrated significant potential in tumor immunotherapy. Currently, nearly one hundred registered clinical trials ini...
mRNA-targeted tumor drugs, featuring various types and distinct antitumor mechanisms of action, have demonstrated significant potential in tumor immunotherapy. Currently, nearly one hundred registered clinical trials initiated by pharmaceutical companies or sponsors are being conducted worldwide, and these trials have shown impressive efficacy. In China, multiple registered clinical trials have been conducted in the past two years, reflecting rapid development. Although mRNA tumor drugs still face certain challenges, the advantages of mRNA technology are clear, promoting the integrated development of immunotherapy, gene therapy, and cell therapy. With ongoing technological innovation and optimization, the demand for "mRNA therapy" for tumor prevention and treatment is expected to be met. This review discusses the distinct mechanisms of action, progress in basic research, clinical advancements, challenges, and future perspectives of both prophylactic and therapeutic mRNA cancer vaccines. It aims to enhance understanding of research progress in mRNA technology for oncological drug applications and to provide insights for future research and product development of mRNA-based interventions in oncology.
[The Association Between Depressive Symptoms and Multimorbidity Among Middle-Aged and Older Adults in Rural China: Sequential Mediation Effects of Pain and Activities of Daily Living].
Li C, Lai H, Pan J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834981 · Full text
OBJECTIVE: To investigate the sequential mediation effects of pain and activities of daily living (ADLs) on the relationship between multimorbidity and depressive symptoms among middle-aged and older adults in rural Chin...
OBJECTIVE: To investigate the sequential mediation effects of pain and activities of daily living (ADLs) on the relationship between multimorbidity and depressive symptoms among middle-aged and older adults in rural China, providing evidence to optimize health management strategies. METHODS: Using four waves of data (2011-2018) from the China Health and Retirement Longitudinal Study (CHARLS), cross-sectional logistic models and panel random-effects models were used to examine the associations and effects of multimorbidity, pain, ADLs, and depression. Structural equation modeling (SEM) combined with the Bootstrap method was applied to analyze the mediation pathways. RESULTS: A total of 2949 middle-aged and older participants were included in the study. When instrumental activities of dailyliving (IADLs) were used as the mediator, approximately 48.44% of the association between comorbidity and depression was attributable to the mediating path of physical pain and IADLs (0.051, 95% CI: 0.045-0.056). When basic activities of daily living (BADLs) were used as the mediator, approximately 47.91% of the association between comorbidity and depression was attributable to the mediating path of physical pain and BADLs (0.050, 95% CI: 0.045-0.056). The chain mediating pathways involving physical pain and the development of daily living abilities were both statistically significant (0.005, 95% CI: 0.004-0.006). CONCLUSION: Multimorbidity directly increases the risk of depression and also exacerbates this risk through pain and subsequent declines in ADLs, either independently or sequentially.
[Research on Bacteriophage Resistance: Coevolutionary Arms Race Between Bacteria and Phages Drives Novel Antibacterial Therapies].
Li R, Wan F, Wei J … +5 more , Wen Y, Jia P, Lin P, Liu L, Wu M
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834980 · Full text
The growing threat of antimicrobial resistance requires the urgent development of new therapeutic strategies and agents. Bacteriophage therapy offers a promising alternative, utilizing phages' ability to specifically rec...
The growing threat of antimicrobial resistance requires the urgent development of new therapeutic strategies and agents. Bacteriophage therapy offers a promising alternative, utilizing phages' ability to specifically recognize and lyse bacterial cells. The ubiquity of bacteriophages subjects bacteria to constant evolutionary pressure, driving the emergence of diverse systems that defend against phage infection. The repertoire of phage defense genes includes a wide range of functions, such as nucleases, helicases, and ATPases. Host-phage interactions are complex and multifaceted. Bacterial defense mechanisms operate at various levels: initial innate defenses that inhibit phage adsorption, block nucleic acid injection, and interfere with virion assembly; early vesicle rupture targeting phage nucleic acids; systems that specifically target phage DNA and RNA; and abortive infection, which results in the degradation of bacterial nucleic acids, depletion of NAD, and changes in cell membrane integrity. Notably, abortive infection prevents phage propagation, though at the cost of bacterial cell death. Although many defense systems have been predicted and identified through bioinformatics, the precise molecular mechanisms and detailed pathways of most systems remain poorly understood. Future research should focus on clarifying the exact molecular mechanisms, regulatory networks, distribution patterns, and roles in bacterial fitness for both newly discovered and established defense systems. Such insights are essential for developing innovative strategies to combat bacterial infections. This review examines the core mechanisms and application potential of bacterial antiphage defense. It systematically summarizes four key aspects of the bacterial antiphage defense system: early infection defense, phage nucleic acid-targeted defense, abortive infection defense, and transferable defense strategies. It also highlights current bottlenecks in the field, such as unclear defense mechanisms, insufficient clinical transformation technologies, and risks associated with the transferability of defense systems. Corresponding countermeasures and suggestions are proposed, including in-depth mechanistic research, construction of defense profiles for pathogenic bacteria, and development of engineered phages and synergistic therapies, to provide references for optimizing phage therapy and innovating bacterial infection treatment, thereby offering new perspectives for treating bacterial infections.
[Aging-Associated Oral Microbiota Dysbiosis and Hypofunction: Their Role in Alzheimer's Disease Pathogenesis].
Li Y, Chen Y, Chen S … +1 more , Huang X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834979 · Full text
Alzheimer's disease (AD), a multifactorial neurodegenerative condition, imposes a major burden on societies with aging populations. Recent research indicates that oral cavity health is a critical factor influencing AD pa...
Alzheimer's disease (AD), a multifactorial neurodegenerative condition, imposes a major burden on societies with aging populations. Recent research indicates that oral cavity health is a critical factor influencing AD pathology, making proactive investigation of this modifiable risk factor essential. This review proposes that aging-related oral microecological dysbiosis and oral hypofunction may promote AD progression by inducing or exacerbating systemic inflammation and disrupting the homeostasis of the "oral-gut-brain" axis. Moreover, each factor may worsen damage through distinct biological pathways: oral microbiota dysbiosis allows direct invasion of the central nervous system by oral pathogens, promoting amyloid β-protein (Aβ) deposition and Tau hyperphosphorylation, while chronic sensory deprivation from oral dysfunction triggers neuronal degeneration and adverse remodeling in key cognitive brain regions. This review aims to systematically elucidate the roles of oral microbiota dysbiosis and oral hypofunction in AD pathogenesis in the context of aging, clarify their underlying biological mechanisms, and explore the potential value of integrating oral cavity health management into comprehensive AD prevention and treatment strategies.
[Exploration of Different Methods for Constructing Animal Models of Melasma in Plateau Regions].
Danzengdeqiong, Fang L, Nizhen … +6 more , Wang J, Duojizhuoma, Yangla, Suolangwangdui, Deyang, Basangzhuoma
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834978 · Full text
OBJECTIVE: To investigate suitable methods and efficacy for establishing a melasma animal model under natural high-altitude conditions (Lhasa City, 3650 m) using three approaches: the commonly used progesterone injection...
OBJECTIVE: To investigate suitable methods and efficacy for establishing a melasma animal model under natural high-altitude conditions (Lhasa City, 3650 m) using three approaches: the commonly used progesterone injection plus UV lamp irradiation (Model Ⅰ), and our research group's novel methods of progesterone injection plus natural light irradiation (Model Ⅱ) and natural light irradiation alone (Model Ⅲ). The goal is to provide experimental technical support for subsequent basic research. METHODS: Twenty female SPF Kunming mice, aged 6-8 weeks and weighing 18-22 g, were selected in the plateau environment. The mice were randomly divided into four groups: progesterone injection plus UV lamp irradiation (Model Ⅰ), progesterone injection plus natural light irradiation (Model Ⅱ), natural light irradiation alone (Model Ⅲ), and a control group (Control), with five mice per group. The apparent status of mice in each group was observed. From days 29 to 40 after modeling, skin physiological parameters of all mice were measured every 2 days, including erythema index (E), melanin (M), skin type angle (ITA), skin lightness (L), redness (a), yellowness (b), and stratum corneum moisture content (CM). After 40 days of modeling, the following biochemical indicators were measured in serum, liver tissue homogenate (LTH), and skin tissue homogenate (STH) from each group: malondialdehyde (MDA), superoxide dismutase (SOD), tyrosinase (TYR), and other biochemical indicators. Correlation analysis was performed between skin physiological parameters and biochemical indicators (based on Model group Ⅰ data). RESULTS: Compared with the Control group, the skin irradiation sites in the three Model groups showed dryness and marked hyperpigmentation. Mice in the Model groups also showed blunted responses, wet bedding, increased food intake, and increased urination, while the Control group showed no skin lesions. Skin physiological parameter results indicated that CM values in Model groups Ⅰ, Ⅱ, and Ⅲ were lower than those in the Control group ( < 0.05); ITA values in Model groups Ⅰ and Ⅲ were lower than in the Control group ( < 0.05); E and a values in all three Model groups were higher than in the Control group ( < 0.05); L values in Model group Ⅱ were lower than in the Control group ( < 0.05); and b values in Model groups Ⅰ and Ⅲ were higher than in the Control group (<0.05). Biochemical indicators showed that, compared with the Control group, Model group Ⅰ had increased MDA levels ( < 0.001), while Model group Ⅲ had elevated MDA levels ( < 0.05). Additionally, Model group Ⅰ had lower SOD and SOD activities than Model Ⅱ ( < 0.05). Correlation analysis indicated that MDA was positively correlated with E value ( = 0.72), while MDA and MDA levels were negatively correlated with L value ( = －0.63, －0.72). Additionally, SOD activity was positively correlated with ITA ( = 0.59). CONCLUSION: Model Ⅰ demonstrated superior modeling efficacy, while Models Ⅱ and Ⅲ remained incompletely stabilized.
[Study of the Protective Effect and Mechanism of Inclisiran on Renal Tissue in a Type 2 Diabetes Mouse Model via the Transforming Growth Factor-β Pathway].
Li H, Chen B, Xiang X … +7 more , Qi L, Zhang D, Tang Y, Zhang L, Ou S, Xue L, Wu W
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834977 · Full text
OBJECTIVE: To investigate whether the novel proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor Inclisiran exerts protective effects on the kidneys under high-glucose conditions, and to predict whether its me...
OBJECTIVE: To investigate whether the novel proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor Inclisiran exerts protective effects on the kidneys under high-glucose conditions, and to predict whether its mechanism involves the transforming growth factor-β (TGF-β) pathway using proteomic techniques, while constructing its regulatory network. METHODS: Healthy male C57BL/6J mice were randomly assigned to four groups: Group A (control, = 9), Group B (diabetes model, = 9), Group C (diabetes + low-dose Inclisiran, = 9), and Group D (diabetes + high-dose Inclisiran, = 9). Groups B, C, and D were induced with type 2 diabetes using a high-fat diet combined with streptozotocin (STZ). Diabetes was confirmed by three consecutive days of fasting blood glucose levels > 16.7 mmol/L after modeling. The experiment ended 8 weeks after modeling. Renal tissue changes were evaluated using hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining. Serum creatinine, low-density lipoprotein (LDL), cholesterol, and PCSK9 levels were measured, along with 24 h urinary protein-to-creatinine ratios. Renal tissue samples from Groups A, B, and D (4 mice per group) underwent transcriptomic sequencing to identify differentially expressed proteins. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses were performed to assess the potential of Inclisiran to protect the kidneys via the TGF-β pathway. RESULTS: After modeling, blood glucose, urine protein/creatinine ratio, blood creatinine, cholesterol, and other indicators in groups B, C, and D were significantly higher than those in group A, with group B showing the highest values ( < 0.05). In the renal tissues of groups B, C, and D, focal tubular cell degeneration and mesangial proliferation were observed. The glomerular proliferation index in group B was significantly higher than in the other groups. Proteomics identified 1096 differentially expressed proteins (579 upregulated and 517 downregulated) between groups A and B, and 911 differentially expressed proteins (475 upregulated and 436 downregulated) between groups B and D. KEGG enrichment analysis showed that the TGF-β pathway was enriched in both the A-B and B-D group comparisons. There were 11 downregulated differentially expressed proteins (P45481: Crebbp, P70387: Hfe, Q61502: E2f5, Q62312: Tgfbr2, Q62432: Smad2, Q8BSK8: Rps6kb1, Q8BUN5: Smad3, Q8CG19: Ltbp1, Q9CUN6: Smurf1, Q9JKX3: Tfr2, Q9Z1M4: Rps6kb2) related to this pathway between groups B and D. CONCLUSION: Inclisiran may improve the lipid profile of type 2 diabetic mice and reduce the activity of the TGF-β pathway. Its mechanism of action may be related to effects such as extracellular matrix proliferation.
[Research Progress on Quorum Sensing-Regulated Oral Microbial Interaction Networks in Periodontitis].
Li L, Yu Z, Xu Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834976 · Full text
Quorum sensing (QS) is a mechanism by which bacteria communicate and coordinate group behaviors through the secretion of signaling molecules, playing a critical role in oral microbial interactions and the pathogenesis of...
Quorum sensing (QS) is a mechanism by which bacteria communicate and coordinate group behaviors through the secretion of signaling molecules, playing a critical role in oral microbial interactions and the pathogenesis of periodontitis. This review summarizes recent advances in QS-mediated regulation of oral microbial interaction networks in periodontitis, focusing on the molecular mechanisms that modulate biofilm formation, virulence factor expression, and pathogen community dynamics. In addition, novel QS-targeted therapeutic strategies are discussed. Although periodontitis is fundamentally a chronic inflammatory disease driven by oral dysbiosis, the precise mechanisms by which microbes trigger and amplify inflammatory damage through the "community dynamics-host interaction-microenvironment" axis remain unclear. Therefore, exploring novel therapeutic strategies targeting the QS system offers potential targets and innovative approaches for the clinical treatment of periodontitis.
[Advances in the Regulation of Protein Phosphorylation in Streptococcal Cell Wall Synthesis].
Yi S, Peng X, Wang S
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834975 · Full text
Protein phosphorylation is a key mechanism regulating cell wall synthesis in . Serine/threonine kinases (STKs) and the two-component system dynamically regulate peptidoglycan synthesis and assembly through phosphorylatio...
Protein phosphorylation is a key mechanism regulating cell wall synthesis in . Serine/threonine kinases (STKs) and the two-component system dynamically regulate peptidoglycan synthesis and assembly through phosphorylation, profoundly influencing bacterial shape maintenance, division, and drug resistance. Studies indicate that serine/threonine kinases form a core network regulating cell wall stability by phosphorylating key scaffold proteins such as DivIVA and GpsB, making them important potential targets for novel antimicrobial agents. Despite significant research progress, the spatiotemporal dynamics of this phosphorylation network and its interactions with other modification systems, such as acetylation and ubiquitination, remain to be thoroughly elucidated. Future research should integrate frontier technologies such as high-throughput proteomics and AI-based structural prediction to comprehensively elucidate this complex regulatory system, thereby providing novel strategies to address antimicrobial resistance challenges.
[Study on the Inhibitory Effect and Mechanism of Trans-2-Decenal on ].
He L, He Q, Tian H … +3 more , Fu Y, Cai L, Liao J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834974 · Full text
OBJECTIVE: To investigate the inhibitory and bactericidal activities of the trans-2-decenal compound against () and explore its potential mechanism. METHODS: The minimal inhibitory concentration (MIC) and minimal bacte...
OBJECTIVE: To investigate the inhibitory and bactericidal activities of the trans-2-decenal compound against () and explore its potential mechanism. METHODS: The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of trans-2-decenal against clinical isolates and the standard strain ATCC43504 were determined using the broth microdilution method. Changes in MIC values were monitored to assess drug resistance in induced by continuous treatment with trans-2-decenal. The synergistic antibacterial effect of combining trans-2-decenal with antibiotics such as metronidazole (MTZ), clarithromycin (CLA), amoxicillin (AMX), and levofloxacin (LEV) was evaluated using the fractional inhibitory concentration index (FICI). After treating standard strains with trans-2-decenal, scanning electron microscopy, transmission electron microscopy, crystal violet staining, the semi-solid puncture method, and the Berthelot method were used to assess the effects of trans-2-decenal on the morphology, cell structure, migration, and urease activity of . RESULTS: The MIC range for clinically resistant strains C907, C101, R1, R2, R4, R10, R16, R24, R36, and R40 was 8-16 μg/mL, with an MBC range of 16-32 μg/mL. The MIC for the standard strain ATCC43504 was 16 μg/mL, with an MBC of 16 μg/mL.Trans-2-decenal inhibits at low concentrations for short durations and does not easily induce resistance. Trans-2-decenal reduces the required dosage of antibiotics (metronidazole and levofloxacin), showing an additive effect. Trans-2-decenal alters the shape of and promotes bacterial rupture. Furthermore, Trans-2-decenal exerts its effects by altering morphology, inducing bacterial rupture, inhibiting biofilm formation, reducing the number of mature biofilms, and decreasing urease activity and migration ability. CONCLUSION: Trans-2-decenal may inhibit through multiple mechanisms and is unlikely to promote the development of resistance.
[Recent Research Progress and Prospects on Periodontitis Affecting Systemic Comorbidities via the Oral-Gut Axis].
Zhang S, Li Y, Yang Z … +2 more , Huang H, Pan Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834973 · Full text
Periodontitis is a prevalent chronic infectious and inflammatory disease worldwide, which imposes harms extending far beyond the oral cavity. A large body of research has demonstrated that periodontitis is closely associ...
Periodontitis is a prevalent chronic infectious and inflammatory disease worldwide, which imposes harms extending far beyond the oral cavity. A large body of research has demonstrated that periodontitis is closely associated with various systemic diseases, such as diabetes mellitus, cardiovascular diseases, inflammatory bowel disease, and rheumatoid arthritis. Serving as a crucial pathway connecting the oral cavity to the entire body, the oral-gut axis becomes the core mechanism through which periodontitis affects systemic health, primarily via the ectopic colonization of salivary microbiota, intestinal dysbiosis, intestinal barrier disruption, and systemic inflammation. This review summarizes recent studies focusing on how periodontitis influences systemic comorbidities via the oral-gut axis, encompassing clinical studies, animal experimental and research. We summarize the research progress regarding how periodontitis perturbs intestinal homeostasis through ectopic colonization of oral pathogenic bacteria, immunoinflammation, host factor regulation, and metabolic disorders, and eventually affects systemic diseases via the oral-gut axis. This review aims to provide a new perspective for the prevention and treatment of periodontitis-related systemic comorbidities.
[Diagnostic Value of Serological Markers and Quantitative Real-Time PCR in Patients With Invasive Candidiasis].
Md Ali H, Peng L, Yuan Y … +2 more , Zhou X, Ma Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834972 · Full text
OBJECTIVE: To evaluate the clinical diagnostic value of serological markers, including the 1, 3-β-D-glucan test (G-test), mannan (Mn) antigen, Candida mannan IgG antibody (Mn-IgG), and quantitative real-time PCR(qPCR) i...
OBJECTIVE: To evaluate the clinical diagnostic value of serological markers, including the 1, 3-β-D-glucan test (G-test), mannan (Mn) antigen, Candida mannan IgG antibody (Mn-IgG), and quantitative real-time PCR(qPCR) in patients with invasive candidiasis (IC). METHODS: This study included 79 patients with culture-proven IC (IC group) and 83 patients without IC (non-IC group) at West China Hospital, Sichuan University between December 2022 and December 2024. Serum samples were tested for the G test (chemiluminescence), Mn antigen (ELISA), Mn-IgG (chemiluminescence), and qPCR. The diagnostic sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of individual and combined tests were calculated. Diagnostic performance was assessed using receiver operating characteristic (ROC) curve analysis. RESULTS: The sensitivity, specificity, PPV, and NPV of the three serological markers for IC diagnosis when used individually were as follows: G test, 62.03%, 94.87%, 92.45%, and 71.15%; Mn antigen, 65.63%, 86.59%, 79.25%, and 76.34%; Mn-IgG antibody, 31.65%, 87.95%, 71.43%, and 57.48%. Combined detection of the G test, Mn antigen, and Mn-IgG increased sensitivity to 73.44%. Based on ROC curve analysis, the AUCs for diagnosing IC using each serological marker were: G test, 0.862 (95% CI: 0.803-0.922); Mn test, 0.853 (95% CI: 0.790-0.915); Mn-IgG, 0.603 (95% CI: 0.514-0.692). The combined AUCs for G test + Mn test, G test + Mn-IgG, and G test + Mn test + Mn-IgG were 0.875 (95% CI: 0.809-0.925), 0.869 (95% CI: 0.805-0.917), and 0.875 (95% CI: 0.809-0.924), respectively, demonstrating superiority over individual tests. The sensitivity, specificity, PPV, and NPV of qPCR for IC diagnosis were 100.00%, 98.80%, 98.75%, and 100.00%, respectively. Using culture strain identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as the gold standard, qPCR demonstrated diagnostic concordance rates of 100.00% for , 100.00% for , 94.40% for , and 90.00% for . CONCLUSION: The combined use of the G test, Mn antigen, and Mn-IgG antibody increases diagnostic sensitivity and facilitates early diagnosis of IC. qPCR demonstrates excellent diagnostic performance for IC, enabling species-level identification of common species associated with IC and detection of other species. It also shows consistency with clinical culture methods.
[Retrospect and Prospect of Immune Caries Prevention Research in China].
Xu Q, Fan M
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834971 · Full text
Dental caries is one of the most prevalent oral diseases, significantly affecting public oral health. Current preventive measures, such as oral hygiene practices, fluoride application, and pit and fissure sealants, have...
Dental caries is one of the most prevalent oral diseases, significantly affecting public oral health. Current preventive measures, such as oral hygiene practices, fluoride application, and pit and fissure sealants, have not substantially reduced the incidence of dental caries in China. Immunological research on caries prevention in China has a history of over forty years, with considerable achievements and experience, and shows promise for advancing biological caries prevention. This paper reviews immunological research on caries prevention in China from multiple perspectives, including active immunity, passive immunity, and the relationship between steady-state medicine and caries vaccines. It also discusses future development prospects, suggesting that novel adjuvants and carriers are essential for breakthroughs in caries vaccine research.
[Association of Serum Sodium and Potassium Levels With the Risk of Depressive Symptoms During the Second Trimester of Pregnancy : A Cross-Sectional Study].
Cai J, Dong T, Yu C
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834970 · Full text
OBJECTIVE: To investigate the association between serum sodium and potassium levels and the risk of depressive symptoms during the second trimester of pregnancy. METHODS: This study was based on a prospective birth cohor...
OBJECTIVE: To investigate the association between serum sodium and potassium levels and the risk of depressive symptoms during the second trimester of pregnancy. METHODS: This study was based on a prospective birth cohort of pregnant women who registered at Chongqing Maternal and Child Health Hospital, China, between 2018 and 2020. Depressive symptoms were assessed during the second trimester using the Edinburgh Postnatal Depression Scale (EPDS). Serum sodium and potassium levels were extracted from electronic health records. Logistic regression models were used to analyze the association between serum sodium and potassium levels and the risk of depressive symptoms. RESULTS: Among the 1429 participants, the prevalence of depressive symptoms was 52.76%. A significant negative association was observed for serum potassium, while no significant association was found between serum sodium levels and the risk of depressive symptoms. In the fully adjusted model, the highest quartile (Q4) of serum potassium was associated with a lower risk of depressive symptoms compared to the lowest quartile (Q1) (odds ratio [OR] = 0.715, 95% CI: 0.531 to 0.963). CONCLUSION: Within the normal physiological range, serum potassium levels were associated with the risk of depressive symptoms during the second trimester of pregnancy, whereas no significant association was found for serum sodium levels.
[Neutrophil-derived Microvesicles Regulate DNA Methylation of the Gene in Human Fibroblast-like Synoviocytes].
Zhao Z, Tang M, Liang Y … +6 more , Zhang T, Ren Q, Cheng Q, Xiong Y, Ma K, Zhan D
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834969 · Full text
OBJECTIVE: To investigate how neutrophil-derived microvesicles (NDMV) promote gene hypermethylation in fibroblast-like synoviocytes (FLS) by regulating DNA methyltransferases (DNMT). METHODS: Neutrophils were isolated f...
OBJECTIVE: To investigate how neutrophil-derived microvesicles (NDMV) promote gene hypermethylation in fibroblast-like synoviocytes (FLS) by regulating DNA methyltransferases (DNMT). METHODS: Neutrophils were isolated from the peripheral blood of healthy volunteers and stimulated with tumor necrosis factor α (TNFα) to produce NDMV. Primary FLS were cultured to the fourth passage for use. MTT assays determined the optimal NDMV treatment ratios and durations for FLS. Experiments groups included control, TNFα, NDMV, and TNFα + NDMV. Combined bisulfite restriction analysis (COBRA) assessed global methylation levels and methylation at two specific sites. RT-qPCR and Western blot analyzed phosphorylated and non-phosphorylated DNA methyltransferase 1 (DNMT1) expression in each group. RESULTS: No significant cytotoxicity was observed at NDMV∶FLS ratios ≤ 100∶1 ( > 0.05). NDMV treatment for 24 hours had no significant effect on FLS cell viability ( > 0.05). The overall methylation level of the gene in the TNFα group was (37.62 ± 2.38)%, which differed significantly from the control group (58.97 ± 1.50)%, NDMV group (59.59 ± 1.15)%, and TNFα + NDMV group (59.11 ± 0.85)%, < 0.001. Compared with the TNFα group, methylation levels at sites 1 and 2 in the control, NDMV, and TNFα + NDMV groups also showed statistically significant differences ( < 0.01). The relative expression levels of phosphorylated DNMT1 protein in the control group, TNFα group, NDMV group, and TNFα + NDMV group were 1.00 ± 0.01, 0.40 ± 0.01, 2.18 ± 0.02, and 1.58 ± 0.05, respectively ( < 0.001). CONCLUSION: NDMV enhances gene methylation by regulating phosphorylated DNMT1 expression in FLS.
[Association Between Obesity and Dyslipidemia Among Adults in Three Counties (Cities) of Sichuan Province].
Jiang L, Chen Y, Xu X … +1 more , Zhao L
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834968 · Full text
OBJECTIVE: To investigate the association between obesity and dyslipidemia among adult residents in three counties (cities) of Sichuan Province, and to reveal the potential non-linear dose-response relationship between b...
OBJECTIVE: To investigate the association between obesity and dyslipidemia among adult residents in three counties (cities) of Sichuan Province, and to reveal the potential non-linear dose-response relationship between body mass index (BMI) and waist circumference (WC) and the risk of dyslipidemia, providing an empirical basis for developing precise weight management strategies. METHODS: This study was conducted in Dujiangyan City, Pujiang County, and Jiange County of Sichuan Province. A multi-stage cluster random sampling method was used to select communities (townships), households, and participants within each county (city). The survey was conducted in 2023 and included 11561 permanent residents aged ≥18 years. A binary logistic regression model was used to analyze the association between different types of obesity and dyslipidemia. A restricted cubic spline (RCS) model was employed to explore the dose-response relationship between BMI, WC, and dyslipidemia, with the model's goodness of fit assessed by the AIC value. RESULTS: Among the study subjects, the prevalence of dyslipidemia was 29.07%, overweight was 33.50%, obesity was 11.95%, and abdominal obesity was 29.79%. Logistic regression analysis showed that, after adjusting for confounding factors, both obesity (odds ratio [OR] = 1.22, 95% CI: 1.05-1.42) and abdominal obesity (OR = 1.23, 95% CI: 1.11-1.36) were positively associated with dyslipidemia compared to individuals with normal weight. After stratification by gender, the association between abdominal obesity and dyslipidemia was more significant in females (OR = 1.36, 95% CI: 1.18-1.57). The RCS model further revealed that the relationship between BMI and dyslipidemia followed a J-shaped curve, with an inflection point at a BMI of 24.87 kg/m². The relationship between WC and dyslipidemia showed an S-shaped curve, with an inflection point at a WC of 86 cm. This indicates that the strength of the association changes non-linearly as the values of these indicators increase. CONCLUSION: Among adult residents of three counties (or cities) in Sichuan Province, obesity and abdominal obesity are independently associated with dyslipidemia, and the association exhibits non-linearity and female specificity. Weight management strategies should focus on individuals with general and abdominal obesity, particularly females with abdominal obesity. The risk inflection points for BMI and WC should be used as key indicators for early intervention.
[The Relationship Between Serum Very Long-chain Saturated Fatty Acids and Chronic Kidney Disease].
Liu K, Wang Y, Wang L
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834967 · Full text
OBJECTIVE: To investigate the relationship between very long chain saturated fatty acids (VLCSFAs) levels in the serum of American adults and chronic kidney disease (CKD), thereby providing new insights for the preventio...
OBJECTIVE: To investigate the relationship between very long chain saturated fatty acids (VLCSFAs) levels in the serum of American adults and chronic kidney disease (CKD), thereby providing new insights for the prevention and treatment of CKD. METHODS: Using data from the 2011-2014 National Health and Nutrition Examination Survey (NHANES), individuals under 20 years of age and those lacking serum creatinine, blood urea nitrogen, urine albumin-to-creatinine ratio (uACR), or other covariate data were excluded. Estimated glomerular filtration rate (eGFR) was calculated using the CKD-EPI (2009) equation. The independent relationship between VLCSFAs and CKD was examined using weighted multivariate logistic regression, the XGBoost machine learning model, and subgroup analyses. Restricted cubic splines (RCS) were used to test for nonlinear associations. RESULTS: A total of 4164 participants were analyzed. Serum VLCSFAs levels differed significantly between the CKD and non-CKD groups ( < 0.05). In fully adjusted models, weighted multivariate logistic regression revealed a decreasing trend in CKD risk with increasing serum VLCSFAs levels, particularly for docosanoic acid, tricosanoic acid, and lignoceric acid, which significantly reduced CKD risk (docosanoic acid: odds ratio [OR] = 0.17, 95% CI: 0.06-0.43, < 0.001; tricosanoic acid: OR = 0.02, 95% CI: 0.002-0.15, < 0.001; lignoceric acid: OR = 0.13, 95% CI: 0.04-0.40, < 0.001). RCS analysis showed no nonlinear association between VLCSFAs and CKD. The XGBoost machine learning model identified triacontanoic acid as the most important factor for CKD risk. Subgroup analysis indicated that docosanoic acid, tricosanoic acid, and lignoceric acid exerted protective effects only in CKD participants with concomitant hypertension, while showing no impact on those with diabetes, coronary heart disease, or stroke. CONCLUSION: Elevated circulating VLCSFAs levels in US adults are associated with reduced CKD risk. Further large-scale prospective studies are needed to validate these findings.
[Chlorhexidine and Polident Induce the Formation of Persistence].
Lin Y, Cheng L, Zhou X … +3 more , Liao G, Zhu Z, Ren B
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834966 · Full text
OBJECTIVE: To investigate the mechanism by which chlorhexidine and Polident, commonly used denture cleansers, induce the formation of persistence in (), which is the causative agent of denture stomatitis. METHODS: biof...
OBJECTIVE: To investigate the mechanism by which chlorhexidine and Polident, commonly used denture cleansers, induce the formation of persistence in (), which is the causative agent of denture stomatitis. METHODS: biofilms were formed and treated with 0-40000 μg/mL chlorhexidine and Polident. Surviving colonies were counted, and dose- and time-dependent curves were plotted. The distribution of persisters was observed via vital staining, and retention was verified by isolating surviving colonies. Transcriptomic analysis was performed on strains treated with chlorhexidine for 2 and 6 hours to explore the mechanisms of persister formation. Metabolic changes in persisters were assessed using the MTT assay. Exogenous glucose was added to validate the role of denture cleansers in regulating persister metabolism. RESULTS: After treating biofilms with chlorhexidine and Polident, the dose- and time-response curves of viable cells both exhibited a biphasic bactericidal pattern, indicating the formation of persisters. The biofilm structure was disrupted, and persisters were mostly distributed in the middle and upper layers. Transcriptomic analysis showed marked downregulation of the TCA cycle and aerobic respiration, but upregulation of glycolysis. The MTT assay verified that chlorhexidine and Polident suppressed persister metabolism, while the addition of glucose reduced the persister populations; there was a statistically significant difference compared to the control group ( < 0.05). CONCLUSION: High concentrations of chlorhexidine and Polident can induce the formation of persisters, which are mainly distributed in the upper and middle layers of biofilms. The persisters can resuscitate after drug removal, increasing the risk of recurrent infections. shifts from the tricarboxylic acid cycle and aerobic respiration to glycolysis to maintain a low metabolic level. Exogenous glucose can activate their metabolism and decrease the number of persisters.
[Clinical Manifestations of Childhood Systemic Lupus Erythematosus-associated Acute Pancreatitis and Evaluation of the Efficacy of Plasma Exchange Combined With Glucocorticoids].
Xiong L, Guo H
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834965 · Full text
OBJECTIVES: To summarize and analyze the clinical manifestations of acute pancreatitis associated with childhood systemic lupus erythematosus (cSLE) and to evaluate the therapeutic efficacy of plasma exchange combined wi...
OBJECTIVES: To summarize and analyze the clinical manifestations of acute pancreatitis associated with childhood systemic lupus erythematosus (cSLE) and to evaluate the therapeutic efficacy of plasma exchange combined with glucocorticoids. METHODS: This retrospective study analyzed clinical data from cSLE patients diagnosed and treated at West China Second University Hospital from January 2018 to December 2020. Patients were divided into a pancreatitis group (diagnosed with cSLE-associated acute pancreatitis) and a non-pancreatitis group (without this diagnosis), and differences between groups were compared. RESULTS: Among 170 cSLE patients enrolled, 9 were in the pancreatitis group and 161 were in the non-pancreatitis group. The median SLEDAI score was 24.0 (P25-P75: 20.5-29.0) in the pancreatitis group and 18.0 (P25-P75: 14.0-20.0) in the non-pancreatitis group, with a statistically significant difference ( < 0.05). The incidence rates of abdominal pain, vomiting, abdominal distension, diarrhea, acute pericarditis, and macrophage activation syndrome were higher in the pancreatitis group than in the non-pancreatitis group, with statistically significant differences ( < 0.05). Differences in C-reactive protein, hemoglobin, blood urea nitrogen, serum albumin, serum amylase, serum lipase, and lipid levels between the two groups were also statistically significant ( < 0.05). Multivariate logistic regression analysis indicated that each 1-point increase in SLEDAI score was associated with a 37.5% higher risk of developing pancreatitis (OR 1.375, 95% CI 1.121-1.686, =0.002). All children in the pancreatitis group received high-dose glucocorticoids combined with multiple plasma exchanges, followed by immunosuppressive therapy. Nine patients achieved remission from acute pancreatitis within two weeks of treatment. Two patients died within 30 days, but the cause of death was unrelated to acute pancreatitis. CONCLUSION: Acute pancreatitis is a rare but severe complication of cSLE, with higher SLEDAI scores correlating with increased risk of pancreatitis. Early diagnosis and aggressive treatment with glucocorticoids combined with plasma exchange may be associated with improved short-term outcomes.
About
- Frequency
- Sun
- Papers found
- 200
- RSS feed
- Subscribe