Searches
/
Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
Sun
200 papers
[Differences in Oral and Gut Microbiome Composition Among Preschool Children in Urumqi During the Deciduous and Mixed Dentition Periods].
Ma T, Lian B, Wu Z … +1 more , Zhao J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834964 · Full text
OBJECTIVE: To analyze the oral and gut microbial composition in preschool children during the deciduous and mixed dentition stages, and to investigate the characteristics of microbiota across different dentition stages....
OBJECTIVE: To analyze the oral and gut microbial composition in preschool children during the deciduous and mixed dentition stages, and to investigate the characteristics of microbiota across different dentition stages. METHODS: Using 16S rRNA gene-based third-generation sequencing, this study analyzed saliva and fecal samples collected from 10 children with deciduous dentition and 10 with mixed dentition. The composition and differences in oral and gut microbiota between the two groups were compared. RESULTS: The differences in microbial richness (Chao1 index) and evenness (Shannon index) between the oral cavity and intestine were statistically significant ( = 0.001). Firmicutes, Proteobacteria, and Bacteroidetes were the three dominant phyla at both sites. At the genus level, and were abundant in the oral cavity, while and predominated in the gut. o_Lactobacillus (LDA = 5.04, < 0.001) was identified as a marker genus in the oral cavity during the deciduous dentition period, while g_ (LDA = 5.15, < 0.001) served as an oral marker genus in children with mixed dentition. In the gut, marker genera during the deciduous dentition period included o_Bacteroides (LDA = 5.17, < 0.001) and f_Lachnospiraceae (LDA = 5.16, < 0.001), whereas f_Ruminococcus (LDA = 5.09, < 0.001) and g_ (LDA = 5.03, < 0.001) were marker genera during the mixed dentition period. Upregulation of pathways including lactose degradation and nitrate reduction was observed in the oral microbiota, in contrast to the gut microbiota, where enhanced activity of the methanogenesis from acetate pathway was noted. CONCLUSION: The composition of oral and gut microbiota varies among children at different dentition stages. As dentition changes, the structural diversity and metabolic functions of both oral and gut microbiota also change.
[Oral Health Status of Obese Children andIntervention Strategies].
Cheng R, Lei L, Zhang R … +2 more , Cai H, Hu T
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834963 · Full text
Against the backdrop of the "Healthy China 2030" strategy and the advancement of the "Three Reductions and Three Healthinesses" initiative, the oral health issues of obese children have received significant attention. Re...
Against the backdrop of the "Healthy China 2030" strategy and the advancement of the "Three Reductions and Three Healthinesses" initiative, the oral health issues of obese children have received significant attention. Research has shown that obese children generally have poorer oral health, with significantly higher rates of dental caries, gingivitis, dental malocclusion, and dental trauma compared to children of normal weight. This paper proposes multidimensional measures to improve oral health in obese children, using the "Three Reductions and Three Healthinesses" as the core framework: "reducing salt, oil, and sugar" can lower the risk of oral diseases at the source, while the coordinated promotion of "oral health, healthy body weight, and bone health" can create synergy between oral and overall health. Future efforts will leverage multidisciplinary collaboration, artificial intelligence, and "Internet Plus" technologies to establish a health education system that integrates communities, schools, and families. Implementing tiered management and targeted prevention will enhance both oral and overall health among obese children, contributing to the strategic goals of "Healthy China".
[Whole Exome Sequencing Identified Novel Pathogenic Mutations of in Patients With Oligoasthenozoospermia].
Wang Q, Yang Y, Jiang C … +1 more , Shen Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834962 · Full text
OBJECTIVE: To systematically analyze the mutation spectrum of the androglobin gene () in infertile males and investigate the association between its genotypes and phenotypes related to male infertility, thereby elucidati...
OBJECTIVE: To systematically analyze the mutation spectrum of the androglobin gene () in infertile males and investigate the association between its genotypes and phenotypes related to male infertility, thereby elucidating the critical role of ADGB in male reproductive function. METHODS: This study recruited 781 Chinese males diagnosed with primary infertility. Whole exome sequencing (WES) combined with Sanger sequencing was used to screen for and identify potential pathogenic mutations in the gene among infertile males. Bioinformatics analysis of ADGB evolutionary conservation was conducted using MEGA11 software. Western blotting was performed to detect ADGB expression in patients' sperm. Semen analysis and modified Papanicolaou staining were used to evaluate sperm motility and morphological characteristics, while transmission electron microscopy (TEM) was used to observe abnormalities in sperm ultrastructure. Additionally, real-time fluorescence quantitative PCR was used to detect mRNA expression levels in various mouse tissues and testicular developmental stages. Immunofluorescence staining was used to analyze the localization and expression distribution of ADGB in human and mouse germ cells across different developmental stages. Finally, proteins associated with ADGB were screened using STRING data and validated by co-immunoprecipitation. RESULTS: Among 781 infertile men, 148 gene mutation sites were identified. According to the American College of Medical Genetics and Genomics (ACMG) Variant Classification Guidelines, 45 sites were classified as benign mutations, one site as a potentially benign mutation, 96 sites as variants of uncertain significance, and 6 sites as potentially pathogenic mutations. Two potentially pathogenic mutations (c.4859G > A and c.4981A > G) were identified in a compound heterozygous state in a patient with idiopathic oligoasthenoteratozoospermia (OAT). Conservation analysis suggested that these mutations may disrupt functionally conserved domains of the gene across species, indicating potential pathogenicity. Western blot analysis revealed absent ADGB protein expression in the patient's sperm. Morphological analysis showed marked head abnormalities (microcephaly, conical heads) and tail defects (short tails, acutely angled curved tails, coiled tails, and tailless sperm) in the patient's sperm. TEM observations showed spermatozoa with acrosome loss, disorganized mitochondrial sheath helical structures, and disrupted or partially absent "9 + 2" microtubule structures in the axoneme. Additionally, ADGB was found to be highly expressed after puberty in both human and mouse testes and was localized to the acrosome and flagella of spermatogenic cells. Co-immunoprecipitation experiments confirmed TTC29 and CFAP47 as interacting proteins of ADGB. The patient carrying the pathogenic mutation achieved a successful pregnancy through intracytoplasmic sperm injection (ICSI) treatment. CONCLUSION: This study expands the mutation and phenotypic spectrum of ADGB in male infertility, confirming its biological significance in maintaining human fertility.
[Quantitative Assessment of Microvascular Changes in Diabetic Retinopathy and Their Association With Blood-Retinal Barrier Impairment].
Lei Y, Lü H, Zhou Q … +2 more , Tian M, Cao Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834961 · Full text
OBJECTIVE: To quantitatively evaluate retinal microvascular changes in patients with diabetic retinopathy (DR) using optical coherence tomography angiography (OCTA), and to explore their association with blood-retinal ba...
OBJECTIVE: To quantitatively evaluate retinal microvascular changes in patients with diabetic retinopathy (DR) using optical coherence tomography angiography (OCTA), and to explore their association with blood-retinal barrier (BRB) disruption. METHODS: A total of 208 patients with type 2 diabetes and DR underwent OCTA to obtain microvascular parameters. Serum vascular endothelial growth factor (VEGF) and intercellular adhesion molecule 1 (ICAM-1) levels were measured. Correlations were analyzed, risk factors were identified using logistic regression, and diagnostic efficacy was evaluated with ROC curves. RESULTS: The superficial capillary density (SCP-D) and deep capillary density (DCP-D) of the 208 DR patients were (42.67 ± 4.35)% and (47.89 ± 5.02)%, respectively. The mean values for the area, perimeter, and circularity index of the foveal avascular zone (FAZ) were (0.38 ± 0.10) mm², (2.04 ± 0.28) mm, and 0.72 ± 0.08, respectively. The mean area of the non-perfusion zone was (1.87 ± 0.45) mm². Among these patients, 121 (58.17%) cases had abnormal SCP-D (< 45%), 114 (56.25%) cases had abnormal DCP-D (< 50%), 88 (42.31%) cases had abnormal FAZ area, 77 (37.02%) cases had abnormal FAZ perimeter, 69 (33.17%) cases had abnormal FAZ circularity index, and 142 (68.27%) cases had abnormal non-perfusion zone area. The FAZ area was positively correlated with VEGF ( = 0.559, 95% CI: 0.457-0.661) and ICAM-1 ( = 0.411, 95% CI: 0.289-0.533). The FAZ circularity index, SCP-D, and DCP-D were negatively correlated with VEGF and ICAM-1 ( < 0.05). The area of the non-perfusion zone was positively correlated with both. Logistic regression showed that the duration of diabetes (odds ratio [OR] = 1.159, 95% CI: 1.060-1.267) and VEGF (OR = 1.013, 95% CI: 1.005-1.022) were independent risk factors for severe retinal microvascular changes ( < 0.05). Among the four OCTA assessment indicators, the area of the non-perfusion zone had the highest predictive value, with an area under the curve (AUC) of 0.879 (95% CI: 0.820-0.938). CONCLUSION: The OCTA assessment indicators in DR patients are closely related to BRB-related markers. The area of the non-perfusion zone has the highest predictive value for severe retinal microvascular changes in DR patients.
[Analysis and Projection of the Burden of Chronic Respiratory Diseases Attributable to Smoking in China, 1990-2021].
Xie X, Lin D, Li M … +4 more , Ye X, Fang J, Zhang X, Xue A
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834960 · Full text
OBJECTIVE: To analyze trends in the disease burden of chronic respiratory diseases (CRD) attributable to smoking in China from 1990 to 2021 and to project its development from 2022 to 2035, providing scientific evidence...
OBJECTIVE: To analyze trends in the disease burden of chronic respiratory diseases (CRD) attributable to smoking in China from 1990 to 2021 and to project its development from 2022 to 2035, providing scientific evidence for optimizing tobacco control strategies and public health decision-making. METHODS: Using Global Burden of Disease (GBD) 2021 data, epidemiological data on smoking-attributable CRD were collected for China, Japan, the European Union, the United States, and the global population from 1990 to 2021. Indicators included years lived with disability (YLDs), years of life lost (YLLs), and disability-adjusted life years (DALYs), covering individuals aged 30 years and older. Joinpoint regression models estimated average annual percentage changes (AAPCs), while Bayesian age-period-cohort (BAPC) models projected future trends. RESULTS: In 2021, smoking-attributable CRD DALYs in China reached 10320279 (95% uncertainty interval [UI]: 7806610-12875089) person-years, with an age-standardized disability-adjusted life years rate (ASDR) of 522.34 (95% UI: 394.50-653.32) per 100000 person-years. The number of deaths was 566446 (95% UI: 416802-720431), with an age-standardized mortality rate (ASMR) of 31.34 (95% UI: 23.10-39.69) per 100000 population. YLLs and YLDs were 8983486 (95% UI: 6547449-11421727) person-years and 1336793 (95% UI: 981768-1699656) person-years, corresponding to age-standardized rates (ASR) of 458.96 (95% UI: 337.53-582.19) per 100000 person-years and 63.38 (95% UI: 46.17-80.72) per 100000 person-years, respectively. The disease burden showed significant differences by gender and age, with higher risks among males and middle-aged and elderly populations. From 1990 to 2021, all burden indicators in China showed a declining trend, with ASMR and ASDR projected to continue decreasing over the next decade. CONCLUSION: China has made positive progress in CRD prevention and tobacco control, but challenges such as population aging require continued attention. Targeted prevention interventions, particularly for high-risk populations, should be strengthened to reduce disease burden. The experience and challenges documented in China could offer valuable lessons and policy insights for other countries undergoing similar epidemiological transitions.
[Periodontitis and Metabolic Dysfunction-Associated Fatty Liver Disease: From Oral Dysbiosis to Hepatic Metabolic Dysregulation].
Wang M, Chen R, Ke X … +3 more , Gu Y, Zhang Y, Yan F
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834959 · Full text
Periodontitis and metabolic dysfunction-associated fatty liver disease (MAFLD) are both highly prevalent chronic diseases that impose a significant global burden. Recent studies have revealed a close association between...
Periodontitis and metabolic dysfunction-associated fatty liver disease (MAFLD) are both highly prevalent chronic diseases that impose a significant global burden. Recent studies have revealed a close association between them. This article systematically reviews the epidemiological link between periodontitis and MAFLD, as well as their potential underlying mechanisms. Regarding the investigation of underlying mechanisms, the article focuses on two primary pathways. Firstly, the classical hematogenous transmission pathway, in which periodontal pathogens and their mediated inflammatory responses affect the liver through the systemic circulation; secondly, the recently proposed "oral-gut-liver axis" pathway, in which periodontitis alters the oral microbiota, subsequently disrupting gut microbiota and intestinal barrier function, ultimately leading to hepatic metabolic dysregulation. These studies offer new perspectives for understanding the complex relationship between these diseases and provide potential novel strategies for the prevention and treatment of MAFLD.
[The Impact of Oral Microecology on the Development of Inflammatory Bowel Disease].
Wan K, Yang C, Chen N … +1 more , Chen F
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834958 · Full text
This article systematically reviews the associations and mechanisms between the oral microbiome and inflammatory bowel disease (IBD). Relevant literature was retrieved from the PubMed database from January 2000 to Octobe...
This article systematically reviews the associations and mechanisms between the oral microbiome and inflammatory bowel disease (IBD). Relevant literature was retrieved from the PubMed database from January 2000 to October 2025, and 77 articles meeting the criteria for inductive analysis were selected. The results confirm a bidirectional causal relationship between oral dysbiosis and IBD: patients with IBD exhibit reduced oral microbial diversity, increased abundance of pathogenic bacteria (such as Fusobacterium nucleatum and Porphyromonas gingivalis), and a more active oral immune environment. Oral pathogens can migrate to the gut via the oral-gut axis, directly disrupting the intestinal barrier and microbial homeostasis or activating systemic and intestinal immune responses through pathways such as miRNAs carried by exosomes and immune cell migration, thereby exacerbating IBD. However, current studies have several limitations, including small sample sizes, high heterogeneity in conclusions, lack of longitudinal data, and insufficient technical compatibility. Future research should expand investigations into the roles of oral fungi and viruses and integrate multiomics technologies to advance the clinical translation of oral microbiota in IBD diagnosis and treatment.
[Mendelian Randomization Analysis of Potential Molecular Targets for Type 2 Airway Inflammatory Diseases].
Jiang Z, Meng J, Liu S
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834957 · Full text
OBJECTIVE: To systematically identify potential pathological molecular and therapeutic targets for type 2 airway inflammatory diseases using Mendelian randomization (MR) and co-localization analysis. METHODS: This study...
OBJECTIVE: To systematically identify potential pathological molecular and therapeutic targets for type 2 airway inflammatory diseases using Mendelian randomization (MR) and co-localization analysis. METHODS: This study analyzed 4,302 druggable plasma proteins as exposure factors and performed transcriptional MR analysis using their cis-expression quantitative trait loci (cis-eQTL) as instrumental variables. Disease outcome datasets from the UK Biobank and Finnish Cohortwere used for discovery and replication validation, respectively. For proteins successfully validated, cis-protein quantitative trait loci (cis-pQTL) were further used for protein-level MR analysis. By combining co-localization analysis, reverse MR analysis, and mediation analysis, we investigated the association of these plasma proteins with allergic rhinitis (AR), asthma (AS), and nasal polyps (NP). RESULTS: cis-eQTL MR analysis of 2528 proteins identified 10 proteins associated with AR (TLR10, ERBB3, PNMT, etc.), 7 associated with AS (ERBB3, SLC40A1, PRKCQ, etc.), and 3 associated with NP (IL18RAP, AXL, ERBB3). cis-pQTL MR analysis showed that IL18RAP was associated with lower NP disease risk, while ERBB3 was associated with lower risks of AR, AS, and NP. Co-localization analysis supported the association between ERBB3 and AR (pp.H4 = 0.910). Mediation analysis revealed that the associations between ERBB3 and AR/AS were mediated by eosinophils, with mediation effects accounting for 12.51% and 17.64% of the observed associations, respectively. CONCLUSION: This study identified unique and shared molecular targets for type 2 airway inflammatory diseases, with ERBB3 potentially serving as a shared protective factor and biomarker for AR, AS, and NP.
[Ethanol Extract of de Boiss. Improves Chronic Atrophic Gastritis in Rats via the HO-1/Nrf2 and Myd88/AKT/PI3K Signaling Pathways].
Wangjieciren, Lan J, Dawazhuoma … +3 more , Chen M, DU J, Ye B
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834956 · Full text
OBJECTIVE: The ameliorative effects of the ethanol extract of de Boiss. (VTDB) on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced chronic atrophic gastritis (CAG) in rats were investigated, and its mechanism of acti...
OBJECTIVE: The ameliorative effects of the ethanol extract of de Boiss. (VTDB) on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced chronic atrophic gastritis (CAG) in rats were investigated, and its mechanism of action was preliminarily explored. METHODS: Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to analyze the chemical constituents of VTDB. A rat CAG model was established using MNNG induction combined with irregular feeding. Seventy-two healthy male SD rats were randomly divided into six groups: normal control, model, low-dose VTDB (157 mg/[kg·d]), medium-dose VTDB (314 mg/[kg·d]), high-dose VTDB (628 mg/[kg·d]), and positive control (Vatacoenayme) (216 mg/[kg·d]), with 12 rats in each group. HE staining was used to observe pathological changes in the gastric mucosa of each group. ELISA was performed to measure serum levels of pepsinogen Ⅰ (PGⅠ), pepsinogen Ⅱ (PGⅡ), and inflammatory factors (TNF-α, IL-6, and IL-10). Levels of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in gastric tissues were determined. Western blot was used to measure the expression levels of heme oxygenase-1 (HO-1)/nuclear factor erythroid-2-related factor 2 (Nrf2) and myeloid differentiation primary response protein 88 (MyD88)/protein kinase B (AKT)/phosphatidylinositide 3-kinase (PI3K) signaling pathway proteins in gastric tissues. RESULTS: By comparing retention times, mass spectral fragmentation patterns, and matching with the ESI (±)-MS/MS database, a total of 89 chemical constituents-primarily fatty acids, phenolic acids, and coumarins-were identified in VTDB. HE staining indicated that VTDB partially improved gastric mucosal damage in CAG rats. ELISA showed that, compared with the model group, the VTDB-treated groups had increased serum levels of PGⅠ, PGⅡ, and PGR (PGⅠ/PGⅡ) ( < 0.05) and decreased levels of gastrin 17 (G-17) ( < 0.05). In the low-, medium-, and high-dose groups, serum TNF-α levels decreased, and in the low- and high-dose groups, serum IL-6 secretion decreased ( < 0.05), while serum IL-10 secretion increased in the high-dose group ( < 0.001). In the VTDB medium- and high-dose groups, MDA levels in gastric tissue decreased ( < 0.01), and SOD activity increased in the VTDB low-, medium-, and high-dose groups ( < 0.05). Western blot results showed that HO-1 levels increased in the high-dose VTDB group and Nrf2 levels increased in the low-, medium-, and high-dose VTDB groups ( < 0.001). MyD88 levels decreased in the VTDB low- and high-dose groups, while AKT and PI3K levels decreased in the VTDB medium- and high-dose groups ( < 0.05), indicating that VTDB can modulate the expression of HO-1/Nrf2 and MyD88/AKT/PI3K signaling pathway proteins. CONCLUSION: VTDB reduces gastric damage and inflammation in CAG rats by inhibiting lipid peroxidation and inflammatory processes through its effects on the HO-1/Nrf2 and MyD88/AKT/PI3K signaling pathways.
[The Oral Microecological Theory of Dental Caries].
Zhou X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834955 · Full text
The microecological theory represents the current understanding of dental caries etiology, emphasizing that the dynamic balance of the structure and function of the oral microbial community plays a central role in the in...
The microecological theory represents the current understanding of dental caries etiology, emphasizing that the dynamic balance of the structure and function of the oral microbial community plays a central role in the initiation and progression of caries. This theory posits that dental caries is not caused by a single specific pathogen, but rather results from dysbiosis-an imbalance-of the entire oral microecosystem. By redefining caries from an "infectious disease" to an "ecological imbalance disorder," the microecological theory offers a novel perspective for caries prevention, early intervention, and precision treatment. It underscores that maintaining the homeostasis of the oral microecology is more critical than simply eradicating bacteria, and that ecological approaches represent a key strategy for population-level caries prevention. Homeostatic medicine emphasizes that the dynamic equilibrium of the body's internal environment is fundamental to health. As a major microbial habitat and immunological interface, the oral cavity plays a pivotal role in the body's overall homeostatic network. The stability of the oral microbiome is thus a crucial node in systemic homeostasis. Homeostatic medicine provides a systems-oriented framework for understanding dental caries, shifting the paradigm ofcaries management from "fighting pathogens "toward "preserving ecological balance". This integrative approach aims to achieve the broader goal of promoting systemic homeostasis through local oral health promotion.
[Research Advances on Natural Products in Maintaining Oral Microecological Balance].
Liu Y, Ma Q, Meng M … +2 more , Wu Y, Zou J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834954 · Full text
The oral cavity is a complex microecosystem that provides a suitable habitat for diverse microorganisms, including bacteria, archaea, fungi, viruses, and protozoa. The microbial communities in the oral cavity form a stab...
The oral cavity is a complex microecosystem that provides a suitable habitat for diverse microorganisms, including bacteria, archaea, fungi, viruses, and protozoa. The microbial communities in the oral cavity form a stable microecology through complex interactions and environmental adaptation. In a healthy oral microenvironment, various microorganisms maintain microecological balance through signal communication, material exchange, competition and cooperation for resources and environmental conditions, quorum sensing, and environmental adaptation. In contrast, an imbalanced oral microecology can lead to oral diseases such as endodontic and periodontal diseases, and may even affect systemic health. Natural products have attracted significant attention for maintaining oral microecological balance due to their potential antibacterial, anti-inflammatory, biofilm-inhibiting, and microecology-regulating properties. This article systematically reviews the characteristics of natural products, their mechanisms for regulating oral microecological balance, and their application prospects and limitations in oral disease prevention and treatment, providing a reference for the use of natural products in maintaining oral microecological balance.
[Tumor Necrosis Factor-α Induces Activation of the NOD1-RICK-NF-κB Signaling Axis and Inflammatory Cytokine Release in Trophoblasts].
Deng C, Kang H, Li N … +7 more , Xue Z, Deng Y, Wang M, Li L, Li Y, Zhou S, Yu P
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834953 · Full text
OBJECTIVE: To analyze the differential expression of nucleotide-binding oligomerization domain 1 (NOD1) in maternal-fetal interface cells during early and late pregnancy, and to investigate its molecular mechanisms in me...
OBJECTIVE: To analyze the differential expression of nucleotide-binding oligomerization domain 1 (NOD1) in maternal-fetal interface cells during early and late pregnancy, and to investigate its molecular mechanisms in mediating inflammatory responses. METHODS: Immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect mRNA and protein expression in normal first-trimester villi tissues ( = 30), normal early pregnancy decidua tissues ( = 30), and normal full-term placental tissues ( = 30). Human chorionic trophoblast HTR8/SVneo cells were stimulated with tumor necrosis factor-alpha (TNFα) at various concentrations (0, 0.01, 0.1, 1, 10, and 100 ng/mL) and exposure times (24, 48, and 72 h). Real-time quantitative PCR (qRT-PCR) and western blot analysis were performed to detect differential expression of NOD1, receptor-interacting protein (RIP)-like interacting CLARP kinase (RICK), and p65. ELISA was used to analyze the secretion levels of inflammatory cytokines IL-6 and IL-8. Electrophoretic mobility shift assay (EMSA) was conducted to assess the DNA-binding activity of nuclear factor-kappa B (NF-κB) and to investigate the regulatory mechanisms of NOD1 signaling pathways in trophoblasts. RESULTS: NOD1 was expressed in normal first-trimester villi, decidua, and full-term placental tissues, primarily localized in the cytoplasm of trophoblasts, vascular endothelial cells, and decidual cells. The difference in NOD1 expression between full-term placental tissues and first-trimester villi and decidua was statistically significant ( < 0.05), with higher NOD1 expression observed in full-term placental tissues than in first-trimester villi and decidua. TNFα upregulated NOD1 expression in HTR8/SVneo cells in a dose- and time-dependent manner. IL-6 secretion levels positively correlated with TNFα concentration, while IL-6 and IL-8 release exhibited time-dependent responses to TNFα stimulation. Furthermore, TNFα stimulation enhanced RICK and p65 expression and increased NF-κB DNA-binding activity. CONCLUSION: NOD1 is constitutively expressed in trophoblasts and decidual cells at the maternal-fetal interface during normal pregnancy, participating in the regulation of the gestational process. Changes in NOD1 expression correlate with TNFα-induced inflammatory responses. TNFα may trigger inflammatory cytokine release via the NOD1-RICK-NF-κB signaling axis, mediating inflammatory cascades in trophoblasts. These findings provide novel insights into immune regulatory mechanisms during pregnancy.
[Analysis of the Characteristics of the Oral Virome in Metabolic Dysfunction-Associated Fatty Liver Disease].
Tu Y, Niu C, Huang Z
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834952 · Full text
OBJECTIVE: To investigate the characteristics of salivary and supragingival plaque viromes in patients with metabolic dysfunction-associated fatty liver disease (MAFLD), and provide new insights for noninvasive oral scre...
OBJECTIVE: To investigate the characteristics of salivary and supragingival plaque viromes in patients with metabolic dysfunction-associated fatty liver disease (MAFLD), and provide new insights for noninvasive oral screening and ecological intervention for MAFLD. METHODS: This study included 21 MAFLD patients and 20 healthy controls. Saliva and supragingival plaque samples were collected, and metagenomic sequencing was used to analyze the characteristics of the oral virome. RESULTS: The α-diversity and β-diversity of the salivary virome did not differ significantly between MAFLD patients and healthy individuals ( > 0.05). However, compared with healthy individuals, the α-diversity (Shannon index) and β-diversity (Bray-Curtis distance) of the supragingival plaque virome showed significant differences ( = 0.0303, = 0.001). For species with a relative abundance greater than 0.1%, 14 viral species in saliva and 5 in supragingival plaque differed significantly in relative abundance between the two groups ( < 0.05), with multiple Streptococcus phages enriched in the saliva of MAFLD patients. LEfSe and random forest analyses identified potential biomarkers in saliva and supragingival plaque. Receiver operating characteristic (ROC) curve analysis showed strong diagnostic performance for these biomarkers in both saliva (area under the curve [AUC] = 0.9548, 95% CI: 0.8898-1.0000) and supragingival plaque (AUC = 0.8952, 95% CI: 0.7774-1.0000). Spearman correlation analysis revealed associations between viral species in saliva or supragingival plaque and various disease indicators ( < 0.05). Compared with healthy individuals, MAFLD patients showed higher node counts, significant relationship numbers, and average node degrees in the co-occurrence networks of salivary and supragingival plaque viromes. CONCLUSION: Differences in the species composition and structure of the oral virome between MAFLD patients and healthy individuals suggest that oral viral species could serve as potential biomarkers for diagnosing MAFLD.
[Recent Advances in the Interorgan Regulatory Roles of Adipose Tissue].
Li Z, Xu C, Xu R … +2 more , Zhou C, Fan Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834951 · Full text
Adipose tissue, a vital component of the human body, serves multiple functions, including energy storage, thermal insulation, and protection. Fat is a highly active, dynamic organ that communicates with other organs, pla...
Adipose tissue, a vital component of the human body, serves multiple functions, including energy storage, thermal insulation, and protection. Fat is a highly active, dynamic organ that communicates with other organs, playing a crucial role in maintaining health and is closely linked to the onset and progression of diseases. Chronic diseases associated with adipose tissue result from dysregulated inter-organ interactions and have become a major public health challenge. A systematic review of regulatory networks centered on adipose tissue could help overcome the limitations of single-organ studies and identify key inter-organ communication pathways. This article reviews recent advances regarding adipose tissue as a pivotal signaling hub that facilitates inter-organ communication with the liver, gut, brain, and bone, as well as its connection to tumor progression. The review also highlights the applications of cutting-edge technologies such as single-cell sequencing and artificial intelligence in adipose tissue research. Continued exploration of the crosstalk between adipose tissue and other organs holds great potential for disease treatment and health management.
[Research on the Construction of Aesthetic Education in Medical Universities Under the Background of New Medicine].
Li Y, Yang L, Liu X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834950 · Full text
In response to the demand for cultivating interdisciplinary medical talent under the "New Medical Science" initiative and to address challenges such as the marginalization of aesthetic education and its inadequate integr...
In response to the demand for cultivating interdisciplinary medical talent under the "New Medical Science" initiative and to address challenges such as the marginalization of aesthetic education and its inadequate integration with specialized training in medical universities, this study aims to develop a systematic framework for implementing aesthetic education. Grounded in policy directives and theoretical analysis, this research proposes a tripartite aesthetic education model centered on "Life Aesthetics - Medical Humanities - Practical Innovation." This model is fundamentally guided by the spirit of Chinese aesthetic education, seeking to integrate the life aesthetics of "the beauty of vitality" with the medical ethical traditions of "benevolent heart and art" and "great virtue and sincerity," thereby enhancing its cultural identity and practical applicability. Through four practical pathways - curriculum restructuring, pedagogical innovation, cultural immersion, and institutional coordination - aesthetic education is integrated throughout the entire process of medical professional education. The goal is to foster the synergistic development of medical students' aesthetic cognition, humanistic sensibility, and innovative capabilities, ultimately providing a theoretical and practical framework for cultivating a new generation of medical professionals who are clinically competent, ethically grounded, culturally confident, and innovative.
[Epidemiology, Mechanisms, and Clinical Challenges of Respiratory Syncytial Virus Co-infections in Children].
Huang H, Chen H, Qiao L
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 Jan · PMID 41834949 · Full text
Respiratory syncytial virus (RSV) infection remains a leading cause of respiratory illness in infants and young children worldwide, with no widely available specific treatment. Advances in diagnostic technologies have in...
Respiratory syncytial virus (RSV) infection remains a leading cause of respiratory illness in infants and young children worldwide, with no widely available specific treatment. Advances in diagnostic technologies have increased the detection rate of RSV co-infections, but their impact on disease outcomes remains controversial. This work examines the epidemiological characteristics, clinical implications, and potential mechanisms of RSV co-infections. Findings indicate that RSV co-infections with bacteria or adenoviruses lead to more severe clinical symptoms and more complex treatment, while co-infections with other viruses may prolong illness without increasing severity. The specific mechanisms underlying RSV co-infections are still poorly understood. In recent years, significant breakthroughs in RSV vaccine and long-acting monoclonal antibody development have provided new tools for active prevention. Future research should focus on clarifying the molecular mechanisms of co-infections, developing targeted therapeutic drugs and vaccines, and optimizing diagnostic and treatment strategies to reduce the incidence and mortality of RSV-related diseases.
[Protective Effects of Secoisolarciresinol Digucoside on Trans Fatty Acid-Induced Brain Inflammation and Oxidative Stress in Offspring Mice and Changes in Brain-Derived Neurotrophic Factor 28 and Tropomyosin Receptor Kinase B].
Ma S, Zhu R, Jiang M … +1 more , Zhao W
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Nov · PMID 41536687 · Full text
OBJECTIVE: To investigate the protective effects of secoisolarciresinol digucoside (SDG) on trans fatty acid (TFA)-induced brain inflammatory response and oxidative stress in offspring mice, and to explore the roles of b...
OBJECTIVE: To investigate the protective effects of secoisolarciresinol digucoside (SDG) on trans fatty acid (TFA)-induced brain inflammatory response and oxidative stress in offspring mice, and to explore the roles of brain-derived neurotrophic factor (BDNF) 28 and tropomyosin receptor kinase B (TrkB) in this process. METHODS: Female C57BL/6 mice were used in the study. First, pregnant C57BL/6 mice were divided into 5 groups, receiving a normal diet, TFA, low-dose SDG, medium-dose SDG, and high-dose SDG, respectively. After birth, the offspring of the normal diet and TFA groups were subdivided into 2 groups, the normal diet during pregnancy group and the TFA during pregnancy group. The offspring of the low, medium, and high-dose SDG during pregnancy groups were subdivided into 3 groups of low, medium, and high-dose SDG. As a result, the offspring were divided into 13 groups during the lactation period. Only the mother mice were exposed to TFA or SDG intervention. The growth status of the offspring was monitored. After 21 days of lactation, the offspring were sacrificed and the relevant indicators, including pathological changes in the hippocampal region of the brain, levels of tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ), antioxidant levels, and BDNF and TrkB mRNA and protein expression levels, were measured. RESULT: Maternal TFA exposure and SDG intervention did not result in significant differences in the weight, brain weight, and brain weight coefficient of offspring ( > 0.05). TFA exposure during pregnancy and TFA exposure during pregnancy + lactation led to pathological changes in offspring brain tissue, which were ameliorated by SDG intervention. TFA exposure increased the levels of inflammatory factors (IFN-γ and TNF-α) and oxidative stress markers (increased malondialdehyde [MDA] levels and decreased superoxide dismutase [SOD] activity and glutathione/total glutathione ratio [GSH/t-GSH], P < 0.05) in offspring brains. In addition, TFA exposure upregulated the expression of BDNF mRNA (during pregnancy and pregnancy + lactation) and BDNF protein (during pregnancy, lactation, and pregnancy + lactation), while downregulated TrkB protein expression (during pregnancy and pregnancy + lactation). SDG intervention attenuated these abnormalities by reducing the levels of inflammatory factors (low, medium, and high doses during pregnancy and lactation) and oxidative stress (medium and high doses during pregnancy and lactation), downregulating BDNF mRNA and protein expression, and upregulating TrkB protein expression ( < 0.05). CONCLUSION: Maternal exposure to a TFA-enriched environment during pregnancy and lactation can induce varying degrees of structural and functional impairment in the brains of offspring and alter the expression levels of BDNF and TrkB proteins in the offspring brain. SDG intervention during TFA exposure exerts protective effects against brain injury in offspring mice, potentially by regulating BDNF and TrkB protein expression to appropriate levels, reactivating BDNF-TrkB downstream signaling pathways, and alleviating inflammatory and oxidative damage.
[Nuclear Factor I-X Regulates Molecular Pathways Related to Craniofacial Development Through Phase Separation].
Wang J, Fan Y, Xu R … +2 more , Jing J, Zhou X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Nov · PMID 41536686 · Full text
OBJECTIVE: To investigate whether nuclear factor I-X (NFIX) possesses phase separation capability and to elucidate its mechanism in regulating biological functions related to craniofacial development through phase separa...
OBJECTIVE: To investigate whether nuclear factor I-X (NFIX) possesses phase separation capability and to elucidate its mechanism in regulating biological functions related to craniofacial development through phase separation. METHODS: An enhanced green fluorescent protein (EGFP)-tagged full-length NFIX recombinant plasmid was transfected into U2OS cells, and its subcellular localization was observed using confocal fluorescence microscopy. The protein structure of NFIX was analyzed using the Predictor of Natural Disordered Regions (PONDR), a protein analysis instrument, with a focus on identifying intrinsically disordered regions (IDRs) associated with weak interactions required for biomolecular condensate formation. A truncated NFIX-EGFP plasmid lacking the IDR was constructed, and changes in its intracellular localization were observed. Immunoprecipitation-mass spectrometry (IP-MS) was utilized to analyze differences in protein interactions with downstream factors between full-length and truncated NFIX. RESULTS: In the nucleus, full-length EGFP-NFIX formed biomolecular condensates resembling membraneless organelles. PONDR analysis confirmed that NFIX contains an IDR (aa245-494) (IDR2), which is critical for biomolecular condensate formation. Truncation of IDR2 resulted in a diffuse protein distribution in the nucleus. IP-MS revealed that multiple proteins exhibited IDR2-dependent binding specificity and were significantly enriched in pathways related to craniofacial development, such as the MAPK pathway. CONCLUSION: NFIX regulates bone developmental homeostasis not only through canonical transcriptional mechanisms but also through IDR-mediated phase separation. This biomolecular condensate formation mechanism provides new insights into the pathogenesis of NFIX-related craniofacial dysmorphogenesis syndromes.
[Research Progress on the Role and Regulatory Mechanisms of Lipid Metabolism in the Osteogenic/Odontogenic Differentiation of Dental Stem Cells].
An K, Xie J, Zhou X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Nov · PMID 41536685 · Full text
Dental stem cells (DSCs) are a type of adult stem cells derived from teeth and their surrounding tissues. DSCs have attracted significant attention in tooth and bone regeneration research due to their strong osteogenic/o...
Dental stem cells (DSCs) are a type of adult stem cells derived from teeth and their surrounding tissues. DSCs have attracted significant attention in tooth and bone regeneration research due to their strong osteogenic/odontogenic differentiation potential. Recent studies have shown that lipid metabolism plays a crucial role in stem cell biological activities and may even serve as a key determinant of cell fate. However, the specific roles and regulatory mechanisms of lipid metabolism in the osteogenic/odontogenic differentiation of DSCs have not yet been systematically summarized or comprehensively elucidated. Based on the latest research advances, we systematically analyzed the changes in lipid metabolism during the osteogenic/odontogenic differentiation of DSCs and explored the key regulatory functions involved in this process, aiming to provide new perspectives on metabolic regulation and potential intervention strategies for regenerative medicine research.
[Current Status and Effect Pathways of Knowledge, Attitude, and Practice of Medical Care and Prevention Integration Among Medical Professionals].
Li Q, Wang T, Jian W
Sichuan Da Xue Xue Bao Yi Xue Ban · 2025 Nov · PMID 41536684 · Full text
OBJECTIVE: To investigate the changes in knowledge, attitude, and practice (KAP) regarding the integration of medical care and prevention among medical professionals in medical institutions of pilot cities of the collabo...
OBJECTIVE: To investigate the changes in knowledge, attitude, and practice (KAP) regarding the integration of medical care and prevention among medical professionals in medical institutions of pilot cities of the collaboration and integration of medical care and prevention, a national pilot program for infectious disease prevention and control, to examine the effect pathways, and to provide empirical evidence for promoting the implementation of the integration of medical care and prevention and improving relevant policies. METHODS: The participants in this study were medical professionals involved in medical care and prevention integration work. The participants were all from medical institutions of pilot cities for the collaboration and integration of medical care and prevention. The study sample was selected through multi-stage sampling. A questionnaire based on the theoretical framework of the KAP model was designed. Two cross-sectional surveys were conducted-once before the initiation of the pilot program (baseline) and again 12 months after implementation (after implementation). After propensity score matching (PSM), descriptive statistics and hypothesis testing were used to statistically analyze the questionnaire data. In addition, a structural equation model (SEM) was applied to assess the current status and effect pathways of the participants' KAP regarding the integration of medical care and prevention. RESULTS: A total of 11472 responses were collected, and 10627 were included in analysis after PSM, including 5007 before the implementation of the policy and 5620 after the implementation of the policy. No significant differences were observed in demographic variables between the two samples. Baseline scores for knowledge, attitude, and practice regarding the integration of medical care and prevention were 13.32 ± 0.05, 15.62 ± 0.04, and 6.37 ± 0.02 respectively, while the scores at the end of the study were 13.69, 15.74, and 6.51, respectively, all show an increase to a certain degree ( < 0.05). Stratified analysis by institution level revealed relatively significant improvements in knowledge and practice ( < 0.05), but no significant improvement in attitudes ( > 0.05) among medical professionals. Medical professionals in primary medical institutions generally demonstrated superior knowledge, attitudes, and practice frequency compared with those in secondary and tertiary hospitals ( < 0.001). No significant changes in KAP were observed among medical professionals in secondary hospitals ( > 0.05). Regarding the integration of medical care and prevention, the effect of knowledge on practice (standardized path coefficient = 0.496, 95% CI: 0.482, 0.508) was stronger than that of attitude on practice (standardized path coefficient = 0.267, 95% CI: 0.244, 0.290). The direct effect of knowledge on practice (78.0%) was greater than the indirect effect mediated by attitude (22.0%). CONCLUSION: The national pilot program for the collaboration between and integration of medical care and prevention in infectious disease prevention and control has a positive effect on improving the KAP regarding the integration of medical care and prevention among medical professionals. Knowledge of the integration of medical care and prevention is the primary factor influencing practice.
About
- Frequency
- Sun
- Papers found
- 200
- RSS feed
- Subscribe