Journal Of Oleo Science[JOURNAL]
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200 papers
Research on Fatty Acid Demulsification Technology and Mechanism of Peanut OBs.
Liu C, Chen F
J Oleo Sci · 2025 · PMID 40887261 · Publisher ↗
In this study, different fatty acids were used for demulsification of peanut oil bodies (OBs) extracted by aqueous enzymatic method. Demulsification process was optimized, and mechanism of fatty acid demulsification was...
In this study, different fatty acids were used for demulsification of peanut oil bodies (OBs) extracted by aqueous enzymatic method. Demulsification process was optimized, and mechanism of fatty acid demulsification was studied. By screening of fatty acids and optimizing demulsification process, octanoic acid had the best demulsification effect (98.23%). OBs maintained stability through electrostatic repulsion and steric hindrance. Octanoic acid adjusted pH to isoelectric point (pI) of OBs proteins, which made electrostatic charge on OBs surface was almost zero, reduced electrostatic repulsion, resulted oil droplets aggregation and fusion. With the increase of demulsification time, contents of α-helix and β-folding gradually reduced, contents of β-turning and random coil gradually increased, the maximum fluorescence peak of OBs proteins had obvious redshift, showing internal space conformation of proteins was destroyed and protein molecules were unfolded. Interfacial membrane structure of OBs was destroyed, resulted proteins and phospholipid were no longer evenly distributed on OBs surface. OBs could not maintain original stable form, aggregation and fusion occurred between oil droplets, achieving demulsification. Results of this study suggest that octanoic acid demulsify of peanut OBs was high efficiency, which provided theoretical basis for realizing industrialization of extracting peanut oil by aqueous enzymatic method.
Benefits, Challenges, and an Alternative Model of Fish Oil Enrichment in Animal-Based Food Products:A Review.
Sukoco A, Yoshino T, Yamamoto Y
J Oleo Sci · 2025 · PMID 40887260 · Publisher ↗
Fish oil (FO) has garnered attention in recent decades because of its omega-3 fatty acid (n-3 FAs) content, which is essential for healthy functions. However, the broad application of FO in food products has pros and con...
Fish oil (FO) has garnered attention in recent decades because of its omega-3 fatty acid (n-3 FAs) content, which is essential for healthy functions. However, the broad application of FO in food products has pros and cons because n-3 FAs are highly prone to oxidative deterioration, leading to product rejection. Enriching food products with FO is an effective strategy to boost the accumulation of n-3 FAs in the body. The n-3 FAs are considered essential lipids, and their consumption helps maintain normal triacylglycerol and cholesterol levels in the blood, decreases the risk of cancer and cardiac disorders, and augments brain function. The n-3 FAs obtained from FO can be added to animal diets or food products as free FO or protected FO. In this review, we focus on elucidating the benefits and challenges of adding FO to several animal-based foods, such as meat-, egg-, and milk-based products. In addition, we discuss the preparation of edible film/coating-forming emulsions and the design of FO-enriched double-layered edible films/coatings.
Relationship between the Particle Size and the Oxidative Stability of Aqueous Emulsion Prepared from Triacylglycerol Rich in Docosahexaenoic Acid.
Sato-Ishizaki Y, Iguchi A, Someya K … +4 more , Kusumoto I, Kato S, Nakagawa K, Gotoh N
J Oleo Sci · 2025 · PMID 40754428 · Publisher ↗
The oxidative stability of polyunsaturated fatty acid (PUFA) generally decreases with increasing unsaturation, however, the opposite results have been reported for aqueous emulsions prepared from PUFA. One study showed t...
The oxidative stability of polyunsaturated fatty acid (PUFA) generally decreases with increasing unsaturation, however, the opposite results have been reported for aqueous emulsions prepared from PUFA. One study showed that among PUFA (fish oil) emulsions with varying μm-sized particles, the smaller emulsions had better oxidation stability. Thus, particle size may be involved in the mechanism behind the improved oxidative stability of PUFA by emulsification, and PUFA emulsions with even smaller particle sizes (i.e., nm-sized) may exhibit higher stability. To evaluate these hypotheses, once optimizing conditions under which emulsions with different sizes (100-250 nm) can be prepared using docosahexaenoic acid (DHA)-rich algal oil (DAO), these emulsions were subjected to thermal oxidation (40°C, 5 days under light shielding). Based on peroxide value (POV) data, it is apparent that even DHA, which is particularly prone to oxidation, becomes less susceptible to thermal oxidation when emulsified, and that smaller emulsions lead to greater oxidative stability. The high oxidation stability in the smallest emulsion remained high after removal of the antioxidants (tocopherols) from this emulsion. Considering these results together with preliminary data on triacylglycerol (TG) mono-hydroperoxides measured by high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), the physical properties of the emulsion might account for the greater oxidative stability in smaller emulsions, probably due to the rapid termination of the oxidation reaction within the smaller particle in a more substrate-dense environment, rather than to the action of antioxidant tocopherols. Further research will hopefully lead to a solution to the longstanding problem of oxidation of PUFA such as DHA, which has a variety of benefits.
Phytochemical Compositions and Bioactive Properties of Plantago lanceolata L.: Anti-oxidant, Anti-diabetic, and Anti-cholinergic Potentials.
Işık M, Sümer E
J Oleo Sci · 2025 · PMID 40754427 · Publisher ↗
In this study, the phenolic compound profile, antioxidant capacity, and inhibition potential on enzymes associated with diseases such as Alzheimer's disease (AD) and diabetes of Plantago lanceolata L. (PL) extract were i...
In this study, the phenolic compound profile, antioxidant capacity, and inhibition potential on enzymes associated with diseases such as Alzheimer's disease (AD) and diabetes of Plantago lanceolata L. (PL) extract were investigated in detail. Quantitative analysis of phenolic compounds was performed by advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the highest concentration of the phenolic was observed in fumaric acid (4486.80 µg/L). Vanillic acid, kaempferol, resveratrol and caffeic acid were also detected in significant amounts. The antioxidant activities of PL ethanol extract were determined by DPPH, ABTS, FRAP, and CUPRAC methods and revealed moderate radical scavenging and metal-reducing capacities. The PL extract exhibited notable free radical scavenging activity, with inhibition rates of 37.86 ± 2.42% in the DPPH assay and 34.72 ± 2.86% in the ABTS assay. These results revealed that the extract showed an activity close to standard antioxidants such as α-tocopherol. The extract exhibited inhibitory activity against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glucosidase enzymes, with IC values of 8.77 ± 0.52, 7.37 ± 0.53, and 3.63 ± 0.27 µg mL-1, respectively. These findings may indicate that PL extract may have therapeutic potential for disorders such as Alzheimer's disease and diabetes.
Optimization of the Extraction of Antioxidant Components from Curculigo orchioides Gaertn Rhizomes by Ultrasound-Assisted Enzymatic Extraction.
Tran HT, Tran TTA, Ly CG … +3 more , Tran TH, Tran TT, Tran TKN
J Oleo Sci · 2025 · PMID 40754426 · Publisher ↗
This study investigated the application of ultrasound-assisted enzymatic extraction (UAEE) to obtain bioactive compounds from C. orchioides. The major natural compounds, including phenolics, alkaloids, saponins, flavonoi...
This study investigated the application of ultrasound-assisted enzymatic extraction (UAEE) to obtain bioactive compounds from C. orchioides. The major natural compounds, including phenolics, alkaloids, saponins, flavonoids, and terpenoids, were identified in the extracts. Cellulase concentration, temperature, and material/solvent ratio were determined to be the main factors affecting the extraction efficiency. Using Response surface methodology (RSM), the optimal conditions were established to maximize the bioactive compounds. The major natural compounds, including phenolics, alkaloids, saponins, flavonoids, and terpenoids, were identified in the extracts. The optimal parameters included a cellulase concentration of 2.63%, an extraction time of 10 minutes, a solvent ratio of 75.36, and a temperature of 38.86°C. The total polyphenol content was 48.571 mg GAE/g DW (R=0.9903) and the total flavonoid content was 16.664 mg QE/g DW (R=0.9842). The antioxidant activity assessed by DPPH, ABTS, FRAP, and RP assays showed lower values than the positive controls ascorbic acid and trolox. These findings indicate that although the antioxidant potential of C. orchioides extracts is modest, they are still promising as a source of natural bioactive compounds for potential applications in functional foods and pharmaceuticals.
Discovery and Characterization of a Phosphatidic Acid-hydrolyzing Phosphatase from Streptomyces mirabilis, and its Use for Phospholipid Refinement.
Iwasaki Y, Ishihara K, Damnjanović J
J Oleo Sci · 2025 · PMID 40754425 · Publisher ↗
An acid phosphatase gene from Streptomyces mirabilis NBRC13450 was cloned and expressed in a recombinant strain of Streptomyces lividans TK24. The recombinant enzyme, named SmACP, was secreted into the culture medium, an...
An acid phosphatase gene from Streptomyces mirabilis NBRC13450 was cloned and expressed in a recombinant strain of Streptomyces lividans TK24. The recombinant enzyme, named SmACP, was secreted into the culture medium, and subsequently purified to homogeneity. The purified SmACP exhibited an optimal pH range of 5.5-6.5 for hydrolyzing p-nitrophenyl phosphate. Its activity remained unaffected by Ca, Mg, Zn, and EDTA. Notably, SmACP was capable of hydrolyzing a variety of phosphomonoesters, including long-chain phosphatidic acid (PA) and lyso-PA, in addition to other water-soluble monoesters. However, it showed no activity toward phosphodiester-type phospholipids, such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylserine. This substrate specificity highlights SmACP's potential for selectively removing PA from phospholipid mixtures, which could be valuable in industrial phospholipid production. This is the first report on the identification of a secreted PA-degrading phosphatase, including its DNA and amino acid sequences.
Preparation and Characterization of Luminescent Alkyl β-D-galactoside-curcuminoid Dye Composites via Micelle-to-fiber Transition in Aqueous Systems.
Ogawa S, Ekida K, Ueno A … +3 more , Nomura N, Yamamoto K, Niwa K
J Oleo Sci · 2025 · PMID 40754424 · Publisher ↗
The precise understanding and control of the phase behavior of surfactant aqueous systems have enabled the development of diverse materials in the fields of foods, cosmetics, and pharmaceuticals. In this study, we invest...
The precise understanding and control of the phase behavior of surfactant aqueous systems have enabled the development of diverse materials in the fields of foods, cosmetics, and pharmaceuticals. In this study, we investigated aqueous systems of the bio-based surfactants alkyl β-d-galactosides (CnGals, where n represents the chain length; n = 6, 8, 10, 11, and 12). First, we confirmed that CnGals aqueous systems formed micelle solutions upon heating and hydrogel upon cooling. In particular, decyl β-d-galactoside (C10Gal) exhibited a homogeneous fiber morphology at low concentrations (0.1 wt.%), whereas larger n values afforded an inhomogeneous solid matrix and smaller n values required higher concentrations or did not form a fiber structure. In addition, the C10Gal micelles solubilized curcuminoid dyes such as demethoxycurcumin and curcumin and allowed preparing luminescent fibers upon cooling the solution. The removal of water afforded the corresponding C10Gal-dye solid composite with a high luminescence quantum yield. Powder X-ray diffraction and differential thermal analysis suggested the formation of a stable adduct or cocrystal with a unique interaction between the curcuminoid dyes and C10Gal. Therefore, we successfully prepared an environmentally friendly, highly luminescent organic solid via micelle-to-fiber transition of an environmentally friendly surfactant induced by a mild temperature change.
Effect of Flavor Loading Ratio on d-Limonene Release from Spray-Dried Powders.
Takashige S, Watanabe Y
J Oleo Sci · 2025 · PMID 40754423 · Publisher ↗
This study investigates the effect of flavor loading ratio (FL) on the retention and release properties of d-limonene encapsulated by spray drying using maltodextrin as a wall material. Three powders were prepared with d...
This study investigates the effect of flavor loading ratio (FL) on the retention and release properties of d-limonene encapsulated by spray drying using maltodextrin as a wall material. Three powders were prepared with different d-limonene loading ratios (50% (w/w), 40% (w/w) and 30% (w/w)). As the FL increased, d-limonene retention decreased, and the ratio of surface d-limonene increased. The release rate of d-limonene from the powders was found to increase with both higher storage temperature and FL. The release of d-limonene was analyzed separately for the surface oil, which evaporates immediately, and for the d-limonene encapsulated within the powder. The release rate constant and activation energy were determined by applying the Weibull model to the encapsulated d-limonene content. As the FL increased, the activation energy for d-limonene release from spray-dried powder also increased, suggesting that lower loading ratios provide more effective protection of flavor in the powders.
Novel Co-rendering Process for Enhancing Lard Quality-focusing on Oxidative Stability and Fatty Acid Composition of the Co-rendered Oils.
Jithongtong L, Sombutsuwan P, Chumsantea S … +5 more , Whangsomnuek N, Nakornsadet A, Lilitchan S, Ali MA, Aryusuk K
J Oleo Sci · 2025 · PMID 40754422 · Publisher ↗
This study developed co-rendered oils by extracting sesame seed oil (SSO) using lard as a green extraction solvent during the co-rendering of pork fat and ground sesame seeds at 170°C in an air fryer, with varying fat-to...
This study developed co-rendered oils by extracting sesame seed oil (SSO) using lard as a green extraction solvent during the co-rendering of pork fat and ground sesame seeds at 170°C in an air fryer, with varying fat-to-seed ratios (80:20 to 40:60). Compared to lard, which is high in saturated fatty acids (SFA), and SSO, which is rich in polyunsaturated fatty acids (PUFA), the co-rendered oils demonstrated significantly improved fatty acid composition. As the proportion of sesame seeds increased, the oils showed a marked reduction in atherogenic index (AI) and thrombogenic index (TI), decreasing from 0.51 to 0.29 and from 1.27 to 0.84, respectively. The lowest values were observed at the 40:60 fat to seed ratio, with AI at 0.29 and TI at 0.84. Additionally, bioactive compounds from sesame seeds enhanced the oils' antioxidant activity, as indicated by increased DPPH values from 54.03 to 118.91 µg GEAC/100 g oil and ABTS values from 18.81 to 51.15 µg GEAC/100 g oil, in P80 and P40 samples, respectively. All co-rendered oils complied with Codex Alimentarius standards for acid value (AV), ranging from 0.99 to 2.02 mg KOH/g oil, and peroxide value (PV), ranging from 0.27 to 0.60 meqO/kg oil. Principal component analysis (PCA) revealed positive correlations between sesame seed content and antioxidant activity, oxidative stability, and PUFA levels, while higher pork fat content was associated with increased SFA levels and oil yield. The findings suggest that co-rendering pork fat with sesame seeds produces oils with enhanced nutritional value, oxidative stability, and bioactive compounds content, offering a healthier alternative to conventionally rendered lard for food industry applications.
Camelina Oil as Ethiopia's Next Edible Oil: Insights into Chemical Composition and Agro-Ecological Adaptability- A Review.
Kidane B, Hunde W, Makiso Urugo M … +2 more , Tessema F, Teka TA
J Oleo Sci · 2025 · PMID 40754421 · Publisher ↗
Camelina sativa, commonly known as Camelina, has emerged as a promising oilseed crop due to its remarkable adaptability to various environmental conditions and its rich nutritional profile. The high content of dietary po...
Camelina sativa, commonly known as Camelina, has emerged as a promising oilseed crop due to its remarkable adaptability to various environmental conditions and its rich nutritional profile. The high content of dietary polyunsaturated fatty acids (PUFAs), particularly omega-3 fatty acids, including alpha-linolenic acid (ALA), tocopherols, and phytosterols, distinguishes this oilseed and sets it apart from many traditional vegetable oils. Its advantageous agronomic characteristics, such as strong drought resistance, early maturation, low input requirements, and the ability to be cultivated twice in a single season, position Camelina as a viable cash crop capable of meeting diverse market demands ranging from dietary supplements to cosmetics and high-quality animal feed. In Ethiopia, where the vegetable oil market faces significant challenges, approximately 95% of domestic consumption is met through imports, resulting in substantial foreign exchange expenditures. This reliance highlights the urgent need for local oil sources to enhance nutritional diversity and food security. Camelina's adaptability to Ethiopia's diverse agro-ecological zones offers a unique opportunity to cultivate this crop under various climatic conditions, thereby increasing local production and reducing dependency on imports. Despite the growing interest in Camelina's commercialization, there remains a notable lack of awareness and research regarding its potential applications and benefits within the Ethiopian context. The successful integration of Camelina into Ethiopia's agricultural framework could significantly alleviate the current oil shortage while contributing to sustainable agricultural practices and rural development. Future research should prioritize the development of high-yielding varieties suited to Ethiopia's agro-ecologies and cost-effective, locally adapted post-harvest processing techniques to enhance value addition and market competitiveness. Increased investment in research and development, along with targeted policy support, will be essential to fully realizing the crop's potential in enhancing the country's oilseed production and ensuring a sustainable food supply.
Growth Stage-Dependent Polyphenol Profiles and Antioxidant Properties in Medicinal Plants of Iraqi Kurdistan.
Saadatian M, Abdulwahid-Kurdi SJ, Mohammed KS … +1 more , Abdullah RA
J Oleo Sci · 2025 · PMID 40603080 · Publisher ↗
Medicinal plants, rich in polyphenolic compounds, play a crucial role in traditional and modern medicine due to their antioxidant, anti-inflammatory, and disease-preventing properties. This study investigated the polyphe...
Medicinal plants, rich in polyphenolic compounds, play a crucial role in traditional and modern medicine due to their antioxidant, anti-inflammatory, and disease-preventing properties. This study investigated the polyphenol content and antioxidant activity across various growth stages (pre-flowering, flowering, and post-flowering) of four medicinal plants: Primula auriculata L., Stachys vulgaris L., Verbascum spp., and Ajuga oblongata L., cultivated in the Soran region, Erbil Province, Iraqi Kurdistan. Using high-performance liquid chromatography (HPLC), nine types of polyphenols were identified and quantified, including gallic, caffeic, and chlorogenic acids. The results showed significant variations in polyphenol concentrations across different plant species and growth stages. For example, Primula exhibited the highest levels of gallic acid (41.91 mg/kg) during pre-flowering, while Ajuga showed peak chlorogenic acid content (827.62 mg/kg) post-flowering. Antioxidant activity was highest in Ajuga during flowering (75.33%) and lowest during post-flowering. Overall, polyphenol content was positively correlated with antioxidant activity, highlighting the importance of growth stage in optimizing the medicinal benefits of these plants. These findings contribute to the ethnobotanical knowledge of Kurdish medicinal plants and offer insights into the optimal harvest times for maximizing bioactive compounds.
Antioxidation Performance of Essential Oils of Herbs and Spices in White Mineral Oils.
Meydan I, Uyar B
J Oleo Sci · 2025 · PMID 40603079 · Publisher ↗
White mineral oil (paraffin oil) is a colorless, tasteless and hydrophobic oil used in many different industries. The oil is prone to oxidation, which negatively affects its properties and performance. In this study, six...
White mineral oil (paraffin oil) is a colorless, tasteless and hydrophobic oil used in many different industries. The oil is prone to oxidation, which negatively affects its properties and performance. In this study, six different essential oils obtained from herbs and spices (Sage, Laurel, Basil, Rosemary, Thyme, Oregano) were added to pharmaceutical and technical grade white mineral oils at 0.1 - 0.8 % (w/w) concentrations. Light, heat and air were applied to induce and accelerate oxidation. The total acid number, viscosity, and DPPH radical scavenging activity of the oils were measured in addition to the TGA and DSC tests to evaluate the anti-oxidation performance of the additives. The results showed that combined heat and oxygen treatment was the most effective of the three conditions tested to cause oxidation. The highest DPPH scavenging activities were obtained with oregano and thyme essential oils (up to 59% and 54% DPPH increase, respectively) and DPPH% activity increased linearly with increasing essential oil concentration. Thermal analyses, total acid number and viscosity change data also suggested the essential oils of Thyme, Oregano and Laurel as the best antioxidant additives to be used in white oils.
The Interaction of Lysophosphatidylcholine and Various Tissue-derived Biomaterials on Antimicrobial Effects against Methicillin-resistant Staphylococcus aureus.
Tamura M, Matsumoto T
J Oleo Sci · 2025 · PMID 40603078 · Publisher ↗
The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) bacteremia and local wounds continues to be a major global public health concern. Evidence available for the selection of correct antiseptics for preve...
The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) bacteremia and local wounds continues to be a major global public health concern. Evidence available for the selection of correct antiseptics for preventing and treating wound infections are limited. We here examined whether lysophosphatidylcholine (LPC) remains effective as a wound-compatible antiseptic against in vitro cultivated MRSA in the presence of an organic material. The antimicrobial efficacy of LPC against MRSA was investigated in the presence of various substances, namely bovine serum albumin, defibrinated sheep blood, mucin, and sodium hyaluronate, to evaluate the interactions between LPC and these compounds. We noted the inhibitory effects of 3 mL/L sheep blood and 3 g/L albumin, used to simulate a contaminated wound or environmental surface, on LPC. Two higher concentrations of hyaluronic acid (0.5 and 0.05 g/L) affected LPC activity against MRSA after 3 and 6 h of treatment. In the presence of four concentrations of mucin (30, 3, 0.3, and 0.03 g/L), LPC was almost ineffective against MRSA. We here provided information about interactions between several tissue-derived materials and LPC used against MRSA. In presence of some materials, LPC exhibited relatively stable antimicrobial effect. Thus, LPC may be a promising candidate as a wound antiseptic agent.
Investigation of the Impact of Hydrophobic Chains on the Corrosion Inhibition Properties of Triazine-Based Bisquaternary Ammonium Salts.
Cao G, Gao G, Liang P … +4 more , Wang J, Ruan Y, Hu Z, Zhu H
J Oleo Sci · 2025 · PMID 40603077 · Publisher ↗
Surface activity parameters such as critical micelle concentration (CMC), saturation adsorption (Г), and limiting molecular area (A) of three triazinyl bisquaternary ammonium salts with different hydrophobic chain length...
Surface activity parameters such as critical micelle concentration (CMC), saturation adsorption (Г), and limiting molecular area (A) of three triazinyl bisquaternary ammonium salts with different hydrophobic chain lengths (C-2-C, C-2-C and C-2-C) were analyzed from surface tension tests. The results showed that the CMC value of C-2-C was much lower (0.026 mmol/L) than that of C-2-C and C-2-C. The corrosion inhibition performances of three triazinyl bisquaternary ammonium salts on Q235 in 1 mol/L HCl solution were investigated by weight loss measurements and electrochemistry measurements. The results showed that the longer the hydrophobic chain length, the better the corrosion inhibition performance at the same concentration. The corrosion inhibition efficiencies of C-2-C, C-2-C and C-2-C at 0.2 mmol/L were 98.10%, 96.09% and 94.64%, respectively. Surface analysis and quantum chemical calculations showed that the three triazinyl bisquaternary ammonium salts can be adsorbed on the surface of carbon steel effectively to inhibit the corrosion of the carbon steel surface.
Biophysical Insights into the Interaction of Zwitterionic Surfactant (Sulfobetaine 10) with Aggregation-Prone Protein.
Alamri A, Malik A, Khan JM … +3 more , Alhomida AS, Rehman MT, Ola MS
J Oleo Sci · 2025 · PMID 40603076 · Publisher ↗
Protein misfolding and aggregation play crucial roles in several neurodegenerative disorders. In this study, sulfobetaine-10 (SB10) has been demonstrated to stabilize proteins and inhibit aggregation by preserving solubi...
Protein misfolding and aggregation play crucial roles in several neurodegenerative disorders. In this study, sulfobetaine-10 (SB10) has been demonstrated to stabilize proteins and inhibit aggregation by preserving solubility and retaining native-like structures when exposed to reducing agents such as DTT. We have measured changes in turbidity, secondary, tertiary structures, and ThT fluorescence. When SB10 concentration was used above the critical micelle concentration (CMC), it successfully stops hen egg white lysozyme (HEWL) aggregation. Below CMC, SB10 induces aggregation in the DTT reduced HEWL. However, it does not cause aggregated proteins to regain their native structure. SB10's zwitterionic composition restricts its capacity to aid in protein refolding, highlighting the significance of electrostatic interactions in this process. This research indicates that SB10 has promise in therapeutic protein formulations and as a remedy for protein misfolding diseases.
Investigation of Novel Strategies to Improve the Storage Stability and the Antimicrobial Activity of Ozonized Sunflower Oil.
Gu L, Gao Y, Li W … +9 more , Liu J, Wang D, Chi Y, Zhang J, Gai Q, Nazir H, Jia Q, Wang H, Wang L
J Oleo Sci · 2025 · PMID 40603075 · Publisher ↗
Ozonized sunflower oil has been found to have a valuable antimicrobial activity against bacteria, viruses, and fungi. However, delayed cutaneous wound healing, severe adverse skin reactions such as pain, burns, irritatio...
Ozonized sunflower oil has been found to have a valuable antimicrobial activity against bacteria, viruses, and fungi. However, delayed cutaneous wound healing, severe adverse skin reactions such as pain, burns, irritation, and unsatisfactory storage stability have limited its clinical use as it is known that the performance of ozonized sunflower oil is reflected by core indicators such as iodine value (IV), peroxide value (PV), and acid value (AV). Aiming to improve the above characteristics, ozone oxidation conditions such as the oil water content of raw materials, stirring speed, and ozonized time were successfully optimized, and the optimized conditions were 5%, 300 rpm, and 6 h, respectively. Additionally, the UHPLC-Q-Exactive Orbitrap MS was used to identify the compounds of sunflower oil and ozonation sunflower oil, and 10 fatty acids were successfully identified. The results clearly indicated that the ozonized sunflower oil prepared under optimized ozonation conditions achieved a high degree of ozonation (II) and a satisfactory PV/AV ratio. These optimized conditions further proved to be valuable in improving not only the storage stability of ozonized sunflower oil for up to six months but also in ensuring its remarkable antimicrobial effects on Staphylococcus aureus and Candida albicans, with a PV of 165.75 mmol/kg achieving a 100% antibacterial efficacy.
Modulation of Myofibrillar Protein Emulsion Gels by Controlled Oxidized Chicken Fat: Conformational Structure, Gelation Performance and in vitro Digestion.
Gao X, Liu R, Chang J … +5 more , Liu Z, Zhang C, Wang T, Zhang Y, Wang H
J Oleo Sci · 2025 · PMID 40603074 · Publisher ↗
The gel properties of myofibrillar proteins (MPs) emulsions were significantly affected by the oxidation state. In our study, the effect of chicken fat (CF) at different oxidation levels (0-1.2 h) on the conformational s...
The gel properties of myofibrillar proteins (MPs) emulsions were significantly affected by the oxidation state. In our study, the effect of chicken fat (CF) at different oxidation levels (0-1.2 h) on the conformational structure, intermolecular interaction and gelling performance of MP was investigated. Additionally, the functional characteristics of emulsified gels were explored by scanning electron microscopy (SEM) and in vitro digestion. The results showed that moderately oxidized CF could promote the transformation of α-helix to β-structure, enhanced hydrophobic interaction between proteins and increased the viscoelasticity of MPs gel. In contrast, over-oxidized CF would deteriorate the MPs gel network structure through protein aggregation. Finally, moderately oxidized CF (0.6 h) was used to facilitate the optimal MPs gel, whose gastrointestinal digestion capability increased by 6.85%. This study contributed to a comprehensive understanding of the modification of MPs by oxidized CF on the improvement of physicochemical properties, functional qualities of emulsified gels, which provided a new way of exploring the application of controlled oxidation oils in the emulsion-based meat products and even in the oil-rich meat production industry.
Preparation and Characterization of Emulsifier-free, Submicron-sized Monodisperse Rice Bran Wax Particles and their Applicability.
Ogawa S, Ono M, Sugano S … +2 more , Nakano T, Niwa K
J Oleo Sci · 2025 · PMID 40603073 · Publisher ↗
The development of an optimal material design is crucial for rice bran wax (RBW) to achieve significant advancements in value-added byproducts. In this study, emulsifier-free, submicron-sized, monodisperse RBW particles...
The development of an optimal material design is crucial for rice bran wax (RBW) to achieve significant advancements in value-added byproducts. In this study, emulsifier-free, submicron-sized, monodisperse RBW particles were prepared via melt emulsification using a combination of a high-HLB emulsifier-decaglycerin stearate or Tween 20-and a low-HLB emulsifier-glyceryl stearate or Span 20. High dispersibility was achieved due to the zeta-potential generated by the surrounding surfactants, resulting in monodispersed submicron-sized RBW particles. Surface cleaning with ethanol effectively removed surface-adsorbed emulsifiers, yielding emulsion-free RBW particles. Due to the hydrophobic nature of the wax particles, high solvent repellencies were observed, along with the formation of colloidal assemblies such as liquid marbles and W/O Pickering emulsions using cosmetics-applicable liquids. Based on X-ray diffraction analysis and differential scanning calorimetry, RBW was identified as a promising candidate for emulsifier-free submicron-sized particles due to its high crystallinity, which facilitates the effective elimination of emulsifiers.
Spontaneous Demulsification of Pickering Emulsion by Controlling Interfacial Tensions.
Matsubara H
J Oleo Sci · 2025 · PMID 40603072 · Publisher ↗
In recent years, the applications of Pickering emulsions have expanded in cosmetic and biological systems, leading to increased interest in the adsorption behavior of fine particles on oil-water interfaces. Despite this...
In recent years, the applications of Pickering emulsions have expanded in cosmetic and biological systems, leading to increased interest in the adsorption behavior of fine particles on oil-water interfaces. Despite this interest, there are still many unanswered questions regarding the connection between particle adsorption and interfacial tension. To address these gaps in knowledge, our study focused on three main areas: (1) the relationship between changes in liquid-liquid interfacial tension and the physical properties of Pickering emulsions, (2) competitive adsorption of fine particles and surfactants at the liquid-liquid interface, and (3) the particle size dependence of nanoparticle adsorption capability based on measurements of surface (interfacial) tension and its thermodynamic analysis. In this short review, we will discuss the findings from our experiments, particularly the possibility of spontaneous demulsification of Pickering emulsions through the control of interfacial tension.
Tissue Distribution of Astaxanthin Geometrical Isomers in Male Sprague-Dawley Rats after Oral Administration of Astaxanthin Esters Derived from Haematococcus lacustris.
Ghosh A, Ito M, Nishida Y … +1 more , Honda M
J Oleo Sci · 2025 · PMID 40451824 · Publisher ↗
Recent studies have revealed that the biological activity of astaxanthin differs among E/Z-isomers. Therefore, it is essential to investigate the distribution of astaxanthin isomers in the body to comprehensively elucida...
Recent studies have revealed that the biological activity of astaxanthin differs among E/Z-isomers. Therefore, it is essential to investigate the distribution of astaxanthin isomers in the body to comprehensively elucidate their role. However, owing to the technical complexity of astaxanthin isomer analysis, detailed information regarding the precise distribution of isomers in the body remains limited. In this study, food-grade astaxanthin esters derived from Haematococcus lacustris (total Z-isomer ratio of astaxanthin = 34.5%) were administered to male Sprague-Dawley rats, and their plasma response and tissue distribution were investigated. Astaxanthin isomers were analyzed using normal-phase high-performance liquid chromatography, which can accurately measure the isomers. The maximum plasma concentration (C) and area under the curve from time 0 to 24 h (AUC) values of total astaxanthin isomers were determined to be 20.4 ± 4.6 ng/mL (T: 8.3 ± 2.3 h) and 261.8 ± 16.4 ng·h/mL, respectively. The total Z-isomer ratio of astaxanthin in the plasma increased over time and reached approximately 55% at 24 h post-administration. Astaxanthin accumulated in the liver, kidneys, lungs, and testes exhibited a high total Z-isomer ratio (> 44.0%). In plasma and tissues, the predominant astaxanthin Z-isomer was the 13Z-isomer, and minor quantities of 9Z-, 15Z-, and two unidentified astaxanthin Z-isomers (potentially multi-Z-isomers) were observed. The composition of astaxanthin isomers in the plasma and tissues differed substantially from that of the diet. These findings suggest that astaxanthin Z-isomers, particularly the 13Z-isomer, possess higher absorbability than the all-E-isomer, or that a mechanism regulates astaxanthin isomer composition in the rat body.
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