Kokubyo Gakkai Zasshi. The Journal Of The Stomatological Society, Japan[JOURNAL]
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200 papers
[Lymphoepithelial cyst arising in the parotid gland; a report of 3 cases].
Sato Y, Omura K, Harada H … +2 more , Shimamoto H, Sawai T
Kokubyo Gakkai Zasshi · 2008 Oct · PMID 19044017 · Publisher ↗
Lymphoepithelial cysts occur mainly in the lateral cervical region, but rarely in the parotid gland. We report three cases of lymphoepithelial cysts arising in the parotid gland. These patients presented with a mass in t...
Lymphoepithelial cysts occur mainly in the lateral cervical region, but rarely in the parotid gland. We report three cases of lymphoepithelial cysts arising in the parotid gland. These patients presented with a mass in the pre-auricular or infra-auricular region. All masses were mobile and non-tender. The lesions were removed by superficial partial parotidectomy. A definite diagnosis could not be made at initial evaluation, including clinical findings, ultrasound sonography, computed tomography, magnetic resonance imaging, nor radioisotope scintigraphy. Final diagnoses were made by histopathologic evaluation of the excised specimen. In these three cases, histopathologic examination of the mass showed a cystic lesion to be lined by stratified squamous or cuboidal epithelium that was surrounded by a lymphoid tissue with lymphoid follicles, leading to a histopathological diagnosis of lymphoepithelial cyst. The postoperative course was uneventful, and there has been no evidence of recurrence in any of the three cases reported. A review of 52 cases of lymphoepithelial cyst arising in the parotid gland reported in Japanese literature was also made together with the three cases of lymphoepithelial cyst arising in the parotid gland reported here. The results of this review were considered clinical presentation and histopathogenesis of the three cases of lymphoepithelial cyst arising in the parotid gland reported here.
[Effect of high mobility group box 1 (HMGB1) in cultured human periodontal ligament cells].
Hasegawa N
Kokubyo Gakkai Zasshi · 2008 Oct · PMID 19044016 · Publisher ↗
The periodontal ligament (PDL) is a fibrous connective tissue that exists in the cementum and the alveolar bone. Periodontitis is a chronic inflammatory disease that is caused by a bacterial infection in the periodontal...
The periodontal ligament (PDL) is a fibrous connective tissue that exists in the cementum and the alveolar bone. Periodontitis is a chronic inflammatory disease that is caused by a bacterial infection in the periodontal region. This infection increases the production of inflammatory cytokines and causes the destruction of periodontal tissue. High mobility group box 1 (HMGB1) is a nuclear nonhiston DNA-binding protein that is present in many eukaryotic cells. HMGB1 is released actively from macrophages and monocytes stimulated by lipopolysaccharide or tumor necrosis factor-alpha and passively from damaged cells and necrotic cells. Extracellular HMGB1 signals through a specialized receptor for advanced glycation end products (RAGE), toll-like receptor 2 (TLR2), and toll-like receptor 4 (TLR4). According to a recent report, HMGB1 is of concern in periodontitis. The purpose of this study was to examine the effect of HMGB1 in PDL cells. To investigate RAGE, TLR2 and TLR4 mRNA in PDL cells, reverse transcript-polymerase chain reaction experiments were performed. PDL cells were stimulated with HMGB1, with or without anti-RAGE, TLR2 and TLR4 antibodies. IL-6 and IL-11 production was measured using an enzyme-linked immunosorbent assay, and mRNA expression was quantified by real-time PCR. PDL cells expressed RAGE, TLR2 and TLR4 mRNA. Production and mRNA expression of IL-6 and IL-11 were augmented in PDL cells stimulated with HMGB1. In addition, they were also suppressed by anti-RAGE, TLR2 and TLR4 antibodies. In conclusion, PDL cells produce IL-6 and IL-11 in response to HMGB1 via RAGE, TLR2 and TLR4.
[Improvement of outcomes by primary treatment for temporomandibular disorders].
Aoyama S, Kino K, Iwaki H … +1 more , Amagasa T
Kokubyo Gakkai Zasshi · 2008 Oct · PMID 19044015 · Publisher ↗
The aim of this study was to survey the current state of the temporomandibular joint disorder treatment in our clinic and to confirm the therapeutic outcomes. The subjects in this study were recruited from among the pati...
The aim of this study was to survey the current state of the temporomandibular joint disorder treatment in our clinic and to confirm the therapeutic outcomes. The subjects in this study were recruited from among the patients with temporomandibular disorders who attended the Temporomandibular Disorder Special Clinic, Department of Oral Surgery, Hitachi Yokohama Hospital during one year period from February 2007 to January 2008. A total of 100 consecutive outpatients diagnosed with temporomandibular disorders were selected for the study. Pain intensity and the degree to which daily function was limited before and after treatment were evaluated using the Visual Analogue Scale (VAS). The Beck Depression Inventory-II (BDI-II) score was evaluated simultaneously, and the correlation with changes in symptom was evaluated. The median (25th, 75th percentiles) of pain intensity (VAS value) before treated was 45 (25.0, 65.0) and the degree of limitation of daily function (VAS value) was 15 (0.0, 50.0). The median (25th, 75th percentiles) of pain intensity at 4 weeks after was 10 (0.0, 30.0), and significantly lower than that before treatment (p = 0.000). The degree of limitation of daily function was 0 (0.0, 10.0), also significantly lower than that before treatment (p = 0.000). The BDI-II score decreased from 8 (2.0, 11.8) before treatment to 5 (2.0, 11.0, p = 0.024) and showed no significant correlation with the improvement in outcomes by primary treatment for temporomandibular disorders.
[Dynamic state of soft tissues around dentures during oral functions].
Minakuchi S
Kokubyo Gakkai Zasshi · 2008 Oct · PMID 19044014 · Publisher ↗
Abstract loading — click title to view on PubMed.
[Structural features of ectopic bone-like tissue in porous hydroxyapatite blocks].
Hattori S
Kokubyo Gakkai Zasshi · 2008 Jun · PMID 18634458 · Publisher ↗
Calcium phosphate ceramics are widely used as bone substitutes because of their favorable biocompatibility and osteoconductivity. Some studies have reported that porous hydroxyapatite (HA), with its special structure, im...
Calcium phosphate ceramics are widely used as bone substitutes because of their favorable biocompatibility and osteoconductivity. Some studies have reported that porous hydroxyapatite (HA), with its special structure, implanted at ectopic (non-bone) sites, exhibits osteoinductive activity. However, few studies have examined the detailed ultrastructure of mineralized tissue formed in biomaterials like HA. Therefore, it is important to examine whether the mineralized tissue exhibits structural characteristics of normal bone tissue. Thus, in this study, we subcutaneously implanted hydroxyapatite blocks (5 x 5 x 7 mm) into the backs of adult dogs, and performed detailed morphological examination of the bone-like tissue formed, which showed a lamellar structure. Immunohistochemical staining revealed that the matrix fibers of the bone-like tissue were mainly composed of type I collagen. The pattern of crystal deposition in matrix fibers and the structure of osteoblast-, osteocyte-, bone-lining-, and osteoclast-like cells were similar to those of normal bone tissue and osteogenic cells, respectively. These results indicate that cellular events observed in normal bone formation and remodeling occur in bone-like tissue as well, and suggest that bone-like tissue in HA blocks is very similar to bone tissue.
[Observations on the dentino-enamel junction--net-like structures on decalcified dentin surface].
Niimi T
Kokubyo Gakkai Zasshi · 2008 Jun · PMID 18634457 · Publisher ↗
As part of morphological research to investigate the development and function of the human dentino-enamel junction, structural features of net-like structures on the decalcified dentin surface of upper and lower incisors...
As part of morphological research to investigate the development and function of the human dentino-enamel junction, structural features of net-like structures on the decalcified dentin surface of upper and lower incisors were observed by using stereoscopic microscope, and scanning and transmission electron microscopes. The results were as follows : 1) The net-like structures on the decalcified dentin surface were stained by hematoxylin, orange G phosphomolybdic acid, toluidine blue and aniline blue. 2) The majority of net-like structures were covered with a membrane-like structure. 3) An individual reticulation of net-like structures corresponds to the margin of each dentin foveolus. 4) The dentin crystals situated in the vicinity of the dentinoenamel junction were larger than the crystals of usual dentin and were more closely aligned. On the other hand, the enamel crystals in this region were smaller than those in other layers of enamel. The surface dentin in the vicinity of the dentino-enamel junction seemed to be highly calcified. 5) It was possible to clearly demonstrate the outline and distribution of dentin foveolus by using certain dyes on the decalcified specimen. 6) By observing the distribution and structural features of net-like structures in detail by applying this dye method to human and animal teeth, the formation mechanism of the dentino-enamel junction and functions of those teeth were elucidated morphologically.
[Structual changes of the apex region of the tongue in the elderly].
Takahashi T
Kokubyo Gakkai Zasshi · 2008 Jun · PMID 18634456 · Publisher ↗
The purpose of this research was to clarify the morphological changes of the apex region of the tongue in the elderly. Apexes of tongue were removed from 44 Japanese adult cadavers (64-99 years) which were contributed fo...
The purpose of this research was to clarify the morphological changes of the apex region of the tongue in the elderly. Apexes of tongue were removed from 44 Japanese adult cadavers (64-99 years) which were contributed for dental studies at Tokyo Medical and Dental University. These specimens were fixed again with 10% formaldehyde, and then paraffin sections were made from these specimens according to a general method. After several stainings, these sections were observed with a light microscope. The results were as follows : 1) The epithelium, propria mucosae and muscle layers became thin with aging, and the boundaries between these layers became unclear. 2) The tendencies of fibrosis also became remarkable, and the arrangement and distribution of collagen fibers and elastic fibers became disordered in propria mucosae. 3) The numbers of myocytes and myofibrils tended to decrease. 4) The connective tissue which is the outer layer of nerves tended to increase. Moreover, in some nerves, the paths of each neurofibril became tangled. 5) These findings are structural features in the apex of the tongue of the elderly, involving morphological changes of layers, fibrosis, structural changes of myocytes and myofibrils, nerves and neurofibrils in this region. These changes suggest some relationships to functional disorder in motion, pain and paresthesia of the tongue.
[Effects of hyperbaric oxygen treatment on healing of maxillary distraction osteogenesis in beagle dogs].
Kudoh A
Kokubyo Gakkai Zasshi · 2008 Mar · PMID 18421952 · Publisher ↗
Distraction osteogenesis has been widely used even in the craniofacial region. A long fixation time during the consolidation period, however, is a major clinical disadvantage. Hyperbaric oxygen (HBO) has been used to imp...
Distraction osteogenesis has been widely used even in the craniofacial region. A long fixation time during the consolidation period, however, is a major clinical disadvantage. Hyperbaric oxygen (HBO) has been used to improve healing in ischemic wounds. We have recently started applying hyperbaric oxygen to cleft palate patients after maxillary distraction, but there is little basic evidence. We hypothesized that hyperbaric oxygen would enhance the healing of distraction osteogenesis in the cleft palate model in dogs. A bony segment including a canine was transported proximally into an artificial bone defect in the left palate. Three dogs were treated with hyperbaric oxygen for 20 days just after the distraction and three other dogs underwent only the distraction process (control group). Blood flow of the canine pulp in the bone segment was monitored using a laser Doppler flowmeter throughout the experiment. All the dogs were sacrificed on day 100, and radiological analysis using peripheral quantitative CT and histomorphometric evaluations were performed. Blood flow in the HBO-treated group recovered to the original level about 30 days faster than in the control group (p<0.05). Cortical bone mineral density was significantly higher at the distraction site in the HBO-treated group than in the control group (p<0.05). The histomorphometric analysis revealed that the newly formed bone area was also larger in the HBO-treated group than in the control group (p<0.05). These results suggest that hyperbaric oxygen treatment could be useful for early removal of the distraction device in distraction osteogenesis.
[Local application of simvastatin to rat incisor sockets augments bone].
Nishimura K
Kokubyo Gakkai Zasshi · 2008 Mar · PMID 18421951 · Publisher ↗
Decrease of the alveolar ridge after tooth extraction causes difficulties in prosthodontic treatment, especially in implant supported prostheses. Statins, which are inhibitors of cholesterol synthesis and therapeutic dru...
Decrease of the alveolar ridge after tooth extraction causes difficulties in prosthodontic treatment, especially in implant supported prostheses. Statins, which are inhibitors of cholesterol synthesis and therapeutic drugs for hypercholestemia, stimulate BMP-2 expression in osteoblasts. The purpose of the present study was to examine how local application of simvastatin, one of the statins, to the tooth socket after tooth extraction affects alveolar bone. A mixture of lactic acid/glycolic acid copolymer (PLGA), alpha-tricalcium phosphate (alpha-TCP), calcium carbonate was prepared as a carrier for simvastatin. Ninety-six male Wistar rats, 10 weeks old, were used. After right mandibular incisor extraction, they were divided into eight groups and tooth sockets were treated differently: no treatment (control), PLGA carrier only, or PLGA containing 0.1, 0.25, 0.5, 1.0, 2.0, and 4.0 mg simvastatin, respectively. They were sacrificed at 4 and 8 weeks. Bone mineral content (BMC) of the alveolar ridge was measured with dual-energy X-ray absorptiometry and histological analyses were performed. All groups treated with simvastatin showed higher BMC compared to the control group and PLGA carrier group at 4 and 8 weeks. Histological analyses revealed that the thickness of cortical bone of the statin group increased compared to the control group at 4 and 8 weeks; however, newly-formed bone in the tooth socket of the statin group was less than in those of the control group and PLGA carrier group. The results of this study show that local application of simvastatin may enhance bone formation and preserve the alveolar ridge after tooth extraction.
[Effects of oral streptococci on biofilm formation by cariogenic bacteria in dual species cultures].
Tamura S
Kokubyo Gakkai Zasshi · 2008 Mar · PMID 18421950 · Publisher ↗
The effects of oral commensal streptococci (Streptococcus sanguinis, Streptococcus gordonii, Streptococcus mitis, and Streptococcus salivarius) on biofilm formation by cariogenic mutans streptococci (Streptococcus mutans...
The effects of oral commensal streptococci (Streptococcus sanguinis, Streptococcus gordonii, Streptococcus mitis, and Streptococcus salivarius) on biofilm formation by cariogenic mutans streptococci (Streptococcus mutans and Streptococcus sobrinus) were investigated. Cell suspensions were cultured on 96-well microtiter plates coated with or without salivary components (SC), and in flow cell systems coated with SC in tryptic soy broth including 0.25% sucrose without dextrose (TSB). The resultant biofilm formations were stained using safranin or a LIVE/DEAD BacLight Viability Kit, and examined with absorbance at 492 nm or using confocal laser scanning microscopy. Mutans streptococci and S. sanguinis biofilms were formed significantly on the polystyrene surfaces in TSB. Further, in combination cultures, S. sanguinis formed a sufficient biofilm when cultured with S. mutans. However, when S. sanguinis was cultured with S. sobrinus, biofilm formation was slightly inhibited. S. gordonii also inhibited biofilm formation in the culture with S. sobrinus, but not when cultured with S. mutans. S. mitis and S. salivarius collapsed the biofilm morphology and inhibited volume development in some conditions when cultured with S. mutans or S. sobrinus. Biofilm formation by mutans streptococci was challenged and collapsed on the whole by culturing with each of the other oral streptococci. These results indicate that co-culturing of multiple species of mutans streptococci and other oral streptococci has physical effects related to previous attachment and colonization on the surface, as well as biological effects to regulate biofilm formation.
[Tissue regeneration induced by a transgene for bone morphogenetic protein-12 (BMP-12) in a bone defect].
Goto N
Kokubyo Gakkai Zasshi · 2008 Mar · PMID 18421949 · Publisher ↗
BMP-12, other than BMP-2, 4, and 7, is known to induce the formation of tendon/ligament-like tissues. However, the role of the protein in the process of bone or cartilage healing has not been adequately examined. The pur...
BMP-12, other than BMP-2, 4, and 7, is known to induce the formation of tendon/ligament-like tissues. However, the role of the protein in the process of bone or cartilage healing has not been adequately examined. The purpose of the present study was to examine if a plasmid vector encoding BMP-12 would induce tendon/ ligament formation in a rat bone defect model. A lyophilized atelocollagen pellet with 100 microg of a plasmid vector encoding BMP-12 or BMP-2 was implanted in a bone defect of a rat femoral metaphysis. The samples were retrieved at 2, 4 and 8 weeks post-operation and subjected to histology and real-time PCR analysis. BMP-12 transgene indicated prominent fibrogenesis at 2 weeks. Consequently, the tissue was substituted by bone at 8 weeks. The gene expression of collagen type III was up-regulated by BMP-12 gene transfer after 2 weeks, followed by an increase of elastin and Six1 genes after 4 weeks. These genes, which are intimately related to tendon/ligament formation, decreased in expression at 8 weeks in preparation for osteogenesis afterward. On the other hand, the BMP-2 transgene quickly induced the synthesis of bone-related genes such as collagen type I and ALP. It is concluded that BMP-12 can initiate the development of tendon/ligament-like tissue in a rat bone defect at early healing stages. Maintaining such generation of tissue on bone could be useful for modifying periodontal tissue engineering.
[Comparison of gene expression profile of cementoblasts with periodontal ligament cells in mouse mandible with laser capture microdissection].
Yokoyama Y
Kokubyo Gakkai Zasshi · 2008 Mar · PMID 18421948 · Publisher ↗
Cementum is an essential tissue to maintain tooth function and should be closely correlated to tooth root development and periodontal tissue regeneration. However, detailed features of the periodontium including cementum...
Cementum is an essential tissue to maintain tooth function and should be closely correlated to tooth root development and periodontal tissue regeneration. However, detailed features of the periodontium including cementum and specific markers for cementoblasts are unknown. Moreover, the molecular mechanism of periodontal tissue development, homeostasis and regeneration remains unknown. Previous studies have usually examined cementum or periodontalligament (PDL) tissue obtained by manual curettage, resulting in difficulties in isolating pure cementum or PDL. We employed laser capture microdissection (LCM) to isolate cementoblasts and PDL cells from undecalcified frozen sections of murine mandible and to obtain RNA of good quality for subsequent genetic analysis. Over 500 cementoblasts and PDL cells were separately laser captured under microscopy. A bioanalyzer detected peaks of 18S and 28S rRNA both in the laser-dissected cementoblasts and in PDL cells, suggesting that the RNA was of sufficient quality. The RNA samples were amplified due to their small amount and a comparative analysis of mRNA expression by GeneChip showed that about 2,000 genes were differentially expressed between cementoblasts and PDL cells. Both cementoblast-positive and PDL cell-negative genes were serially analyzed by quantitative RT-PCR using RNA samples obtained from mandibles and femurs. Several genes were expressed at higher levels in the mandible than in the femur, suggesting that some might be cementoblast-specific markers. We established a novel experimental system with which to isolate target tissues from single cells in undecalcified frozen sections and to obtain intact RNA. These methodologies could be useful for further investigation of mineralized tissues and to explore tissue-specific factors.
[Influence of residual stress in wrought wire clasps on bending properties].
Shirasu K
Kokubyo Gakkai Zasshi · 2008 Mar · PMID 18421947 · Publisher ↗
The purpose of this study was to assess the influence of residual stress produced after the bending manipulation of a straight wire on flexibility and permanent deformation of wrought-wire clasps. A three-dimensional fin...
The purpose of this study was to assess the influence of residual stress produced after the bending manipulation of a straight wire on flexibility and permanent deformation of wrought-wire clasps. A three-dimensional finite element model of a straight wire (12 mm in length and 1.0 mm in diameter) was created. Elastic and plastic properties of a commercial Co-Cr straight wire measured by means of tensile test were input into the program. We simulated the bending of the clasp shoulder and clasp arm to an angle of 90 degrees or 120 degrees and subsequent unloading processes with spring-back phenomenon. The maximum residual tensile stress appeared at the inside of the bending angle after unloading of the bending force. Thereafter, the clasp models were deflected 0.25, 0.50 mm, and 0.75 mm at the tip to simulate the removal and insertion of a denture. The maximum tensile stress at the inside corner of the bending angle under the deflection was considerably higher than the residual stress before deflection. The stress increase by the deflection was larger at the shoulder than the arm. The load required for deflection was larger in the models with the arm of 120 degrees angle than models with the arm of 90 degrees angle. The results suggest that the permanent deformation of wrought-wire clasps is likely to initiate at the clasp shoulder, while the clasp flexibility is dependent on the bending angle of the clasp arm.
[Response properties of the jaw-closing muscle spindle during decreased occlusal vertical dimension in rats].
Fujita K
Kokubyo Gakkai Zasshi · 2008 Mar · PMID 18421946 · Publisher ↗
The masseter-muscle spindle is regarded as being highly adaptable to increases in the occlusal vertical dimension (iOVD), it is hypothesized that spindle function would adapt to a decrease in occlusal vertical dimension...
The masseter-muscle spindle is regarded as being highly adaptable to increases in the occlusal vertical dimension (iOVD), it is hypothesized that spindle function would adapt to a decrease in occlusal vertical dimension (dOVD) as well. Seventy-five 5-week-old female Wistar rats were divided into Control (n = 25) and Experimental (n = 50) groups; those in the Experimental group received a 2.0-mm composite resin build-up to the maxillary molars. The Experimental group was divided into the resin-removal group (n = 25, build-up resin was removed) and the non resin-removal group (n = 25) 8 weeks later; i. e., when the animals were 13 weeks old. Electrophysiological recordings were obtained from masseter-muscle spindle afferents in 13, 14, 15, 19, 21-week-old rats (n = 5 rats each) under general anesthesia Masseter-muscle spindle sensitivity was significantly lower in the resin-removal group 1 week after resin-removal and for the rest of the observation period. The present results indicate that masseter-muscle spindles may not completely adapt to dOVD and may affect jaw function.
[A clinical perspective of craniosynostosis from an orthodontic point of view].
Moriyama K
Kokubyo Gakkai Zasshi · 2007 Oct · PMID 18027581 · Publisher ↗
Abstract loading — click title to view on PubMed.
[Periodontal tissue regeneration therapy].
Izumi Y
Kokubyo Gakkai Zasshi · 2007 Oct · PMID 18027580 · Publisher ↗
Abstract loading — click title to view on PubMed.
[From psychosomatic dentistry to brain dentistry].
Toyofuku A
Kokubyo Gakkai Zasshi · 2007 Oct · PMID 18027579 · Publisher ↗
Abstract loading — click title to view on PubMed.
[Educational effects of PBL for students in the Faculty of Dentistry--with special reference to the educational effects on dysphagia--].
Uchimiya Y
Kokubyo Gakkai Zasshi · 2007 Mar · PMID 17722468 · Publisher ↗
PURPOSE: Although the importance of student education has long been emphasized and various educational strategies have been attempted, the PBL method is deemed to be effective among them. Recently, the PBL method was the...
PURPOSE: Although the importance of student education has long been emphasized and various educational strategies have been attempted, the PBL method is deemed to be effective among them. Recently, the PBL method was therefore employed in our lecture on dysphagia in order to examine its efficacy. SUBJECTS AND METHODS: Thirty-one female and 35 male students (66 in total, 6th-grade students in the Department of Dentistry) were enrolled in this study which was performed from February 9 to June 29, 2006. The students were allocated into 2 groups, 3-4 per group. Two kinds of educational training program on dysphagia were given by a director/tutor experienced in clinical practice for dysphagia. The first group received a lecture of the conventional type (conventional group), while the latter group received a lecture of PBL (PBL group). In both groups, an identical X-ray film taken during swallowing was evaluated. In addition, the students' impression on the lecture they received was obtained by means of questionnaires. RESULTS: The coincident rate of evaluation on the swallowing image was better in the PBL group than the conventional group on average. Among 23 evaluation items, a coincident rate more than moderate was obtained on 4 and 8 items in the conventional and PBL groups respectively. From the results of the questionnaire survey, it was revealed that the item "motivation" was rated to be significantly better in the PBL than in the conventional group. DISCUSSION: A lecture on dysphagia was given by 2 kinds of educational strategy. From the results of this study, it was revealed that the PBL method was more effective for enhancing the students' motivation on the lecture. In the PBL group, the lecture was given by individual cases with subsequently increased questions and answers and promoted positive participation in the lecture as compared to the conventional group. Furthermore, students' motivation was raised probably due to a good understanding of the case details upon evaluation of the swallowing image with the resultant enhanced rate of coincidence. In order to obtain both educational and enlightening effects simultaneously, an educational program by the PBL method was thus considered to be an effective strategy.
[An effective biological marker to detect oral squamous cancer cells--expression patterns of CK 10, 17, 19 and SCCA mRNA--].
Yoshida Y
Kokubyo Gakkai Zasshi · 2007 Mar · PMID 17722467 · Publisher ↗
A variety of techniques have been employed for the detection of tumor cells in the lymph nodes of patients with oral squamous cell carcinoma (OSCC). Molecular analysis has been applied to detect metastases and several re...
A variety of techniques have been employed for the detection of tumor cells in the lymph nodes of patients with oral squamous cell carcinoma (OSCC). Molecular analysis has been applied to detect metastases and several reports have presented examinations of tumor markers, but no target gene that is completely reliable as a molecular biological tumor marker has been found. This study investigated whether a marker exists that is effective to detect OSCC. A total of 134 samples (biopsy and surgical specimens) from 102 OSCC patients were analyzed. Expression patterns of Cytokeratin (CK) 10, 17, 19 and SCCA mRNA in the normal oral mucosa and OSCC samples were examined using RT-PCR. Statistical analyses showed that significant differences existed in expressions of CK 10, 17, 19 and SCCA between OSCC and the normal mucosa (p<0.05). No correlation was observed between the degrees of histological differentiation of the tumor and CK 17 expression. The CK 17 expression also showed no significant differences depending on sites of primary tumors. Among the CK 10, 17, 19 and SCCA investigated, only CK 17 showed high sensitivity and negative predictive value (NPV). CK 17 might be a good biomarker because of the performance of RT-PCR in detecting mRNA of CK 17 with such high sensitivity and NPV. Further studies using a larger number of OSCC patients and other CKs should be undertaken to establish CK 17 as a useful biomarker.
[Effect of the viscosity of alginate impression materials on the preliminary impression for complete dentures].
Aoyama N, Akiba N, Uchida T
Kokubyo Gakkai Zasshi · 2007 Mar · PMID 17722466 · Publisher ↗
The purpose of this study was to clarify the effect of the viscosity of alginate impression materials on the results of the preliminary impressions for complete dentures. Five experimental alginate impression materials w...
The purpose of this study was to clarify the effect of the viscosity of alginate impression materials on the results of the preliminary impressions for complete dentures. Five experimental alginate impression materials with different viscosity were prepared based on a conventional material. Fifteen complete denture wearers were selected and impressions on using the experimental materials were taken. Impressions were evaluated by the success rates at the mylohyoid ridge area (MR), buccal area (BL) and retromolar pad area (RP). The success rates had a tendency to increase as the viscosity rose at MR and RP. The success rates at BL were not significantly different among the materials. Our results indicate that the viscosity of alginate impression materials has a great effect on the results of preliminary impression for complete dentures, especially at MR. Impression materials with high viscosity may be suitable for recording the anatomical form of the alveolar ridge better than those with low viscosity.
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