International Journal Of Biomedical Science[JOURNAL]
Sun
200 papers
Nitric Oxide on Pathophysiology of SARS-CoV 19: Toward Possible Role of Acupuncture Treatment.
Ma SX
Int J Biomed Sci · 2021 Nov · PMID 35018143
The ongoing outbreak of COVID-19 has quickly become a daunting challenge to global health. In the absence of satisfied therapy, effective treatment interventions are urgently needed. Previous studies have demonstrated th...
The ongoing outbreak of COVID-19 has quickly become a daunting challenge to global health. In the absence of satisfied therapy, effective treatment interventions are urgently needed. Previous studies have demonstrated that acupuncture is effective at relieving common symptoms of COVID-19 including breathlessness, nausea, insomnia, leukopenia, fatigue, vomiting, and abdominal pain. Experiments have shown that nitric oxide (NO) inhibits the replication cycle of severe acute respiratory syndrome (SARS) coronavirus with similar structures of COVID-19. Increase in level of NO by using NO gas inhalation has been shown to restore lung function by reducing airway resistance and improving virus-induced lung infections in SARS patients. Recent case report showed that a medical acupuncturist with symptoms consistent with severe COVID pneumonia achieved full recovery by self-administered medical acupuncture and cupping therapy at home. Clinical features and pathophysiology demonstrated that NO deficiency and endothelial dysfunction contribute to the development of COVID-19. Several studies from different groups consistently demonstrated that acupuncture increases NO synthase expression and induces an elevation of NO production and release in plasma and the local skin regions in both animals and humans. It is suggested that exogenous NO supplies or interventions that induce increasing levels of NO can play an important role in protective effects against inflammation and acute lung injury. This article reviews the rationale for mechanisms of NO induction induced by acupuncture in the possible treatment of COVID-19 and highlights its potential for contributing to better clinical outcomes and improving future clinical studies of acupuncture on treatment of COVID-19.
Successful Myomectomy during Cesarean Section: Case Report & Literature Review.
Ghaemmaghami F, Karimi-Zarchi M, Gharebaghian M … +1 more , Kermani T
Int J Biomed Sci · 2017 Jun · PMID 28824348
Myomectomy is the most common surgery with cesarean section. There is controversy between obstetricians about doing myomectomy with cesarean section. A 29 years old primigravida patient presented with a large lower segme...
Myomectomy is the most common surgery with cesarean section. There is controversy between obstetricians about doing myomectomy with cesarean section. A 29 years old primigravida patient presented with a large lower segment myoma (20 cm.) who underwent myomectomy during cesarean section at the term pregnancy. Myoma weighted 1500 gr. She didn't have intra-operative hemorrhage or any post-partum complications. Seems that there is no absolute contra-indication for myomectomy during cesarean section specially if the surgeon has enough experience and the myoma is large, located at the lower segments.
The Impact of Biophysical Properties of Erythrocytes on their Aggregation.
Elblbesy MA, Moustafa ME
Int J Biomed Sci · 2017 Jun · PMID 28824347
Erythrocytes aggregation takes places under low shear conditions or at stasis. All suggested mechanisms of erythrocytes aggregation indicated the importance role of fibrinogen and other blood proteins in enhanced erythro...
Erythrocytes aggregation takes places under low shear conditions or at stasis. All suggested mechanisms of erythrocytes aggregation indicated the importance role of fibrinogen and other blood proteins in enhanced erythrocyte aggregation. Recently a special attention is given to the cellular factors that may effect on erythrocytes aggregation. The present study inferred the effect of the cellular properties of erythrocytes on their aggregation. In the present study, aggregation index was calculated by a simple microscopic method. Correlations between erythrocytes aggregation index and mean cell volume, osmotic fragility, electrophoretic mobility, and magnetophoretic mobility were studied. The findings of this study indicated that the aggregation index is significatly correlated to mean cell volume, magnetophoretic mobility, osmotic fragility and electrophoretic mobility. Thus, It is concluded that cellular factors should be taken into consideration when studying the mechanism of erythrocytes aggregation.
To Evaluate Effect of Airborne Particle Abrasion using Different Abrasives Particles and Compare Two Commercial Available Zirconia on Flexural Strength on Heat Treatment.
Prasad HA, Pasha N, Hilal M … +4 more , Amarnath GS, Kundapur V, Anand M, Singh S
Int J Biomed Sci · 2017 Jun · PMID 28824346
BACKGROUND AND OBJECTIVE: The popularity of ceramic restorations can be attributed to its life-like appearance, durability and biocompatibility and therefore ceramic restorations have been widely used for anterior and po...
BACKGROUND AND OBJECTIVE: The popularity of ceramic restorations can be attributed to its life-like appearance, durability and biocompatibility and therefore ceramic restorations have been widely used for anterior and posterior teeth. Ceramic restorations have esthetic and biocompatible advantages but low fracture resistance. Since it has high flexural strength and fracture resistance, yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) is the dental material most commonly used for the core of ceramic crowns and fixed dental prosthesis. In spite of improved mechanical properties, acceptable marginal adaptation and biocompatibility the whitish opacity of zirconia is an obvious esthetic disadvantage. The zirconia framework is often veneered with conventional feldspathic porcelain to achieve a natural appearance. However it is difficult to achieve sufficient bond strength between zirconia and the veneering material. Achieving sufficient bond strength between the veneering ceramic and the zirconia core is a major challenge in the long term clinical success of veneered zirconia restorations. The main objective of this study is to evaluate the effect of different surface treatments on the fracture strength of the two commercially available Zirconia namely Ceramill and ZR-White (AMANNGIRRBACH and UPCERA) respectively. METHOD: Two commercially available pre-sinteredyttrium stabilized Zirconia blanks (ZR-White and Ceramill) from AMANNGIRRBACH and UPCERA respectively are used to produce the disc shaped specimens of size (15.2 ± 0.03 mm in diameter and 1.2 ± 0.03 mm thick) from each Zirconia blank. All disc shaped specimens are heated at 1200°C in a furnace for 2 hours to form homogenous tetragonal ZrO. The dimensions of the specimens are measured with a digital caliper (aerospace). The thickness and diameter of each specimen are calculated as the means of 3 measurements made at random sites. 80 discs from each Zirconia blank are divided into ten groups of 8 specimens each. Heat treatment after airborne-particle abrasion using 50 µm AlO particles and 50 µm silica coated AlO are applied to the upper and lower surfaces of the specimens. Each specimen is held under a pressure of 30 psi for 15 seconds at a direction perpendicular to the surface and at a distance of 30mm with an airborne particle abrasion device for the specimens in the airborne particle abraded groups. Heat treatments were performed at a starting temperature of 500°C, heating rate of 100°c/ min, ending at a temperature of 1000°C and 15 minutes holding time without vacuum for the specimens in the group 4, 5, 9 and 10. Airborne-particle abrasion mimicking the preparation for cementation was applied to the lower surfaces with 50 µm alumina and silica coated alumina particles for the specimens in the groups 6, 7, 8, 9 and 10. The specimens were cleaned for 15 minutes in an ultrasonic bath containing distilled water. To determine the fracture strength, a disc of 10 mm diameter was used to place 3 hardened steel balls of 3 mm diameter separated each other by 120 degrees (described in the ISO standard 6872 for dental ceramics). Each specimen was centrally placed on this disc. The lower surface mimicking the internal surface of zirconia was the tension side, facing the supporting device testing, while the upper surface mimicking the external surface of the zirconia core was loaded with a flat punch (1 mm in diameter). A universal testing machine was used to perform the test at a cross head speed of 1mm/min. The failure stress was calculated with the equation listed in ISO 6872. The results were then statistically analyzed. A post hoc test was used for pair wise comparisons. RESULT: The mean fracture strength of commercially available Zirconia Ceramill (AMANNGIRBACH) showed a significant higher value compared to the ZR-White (UPCERA) Zirconia (<0.001), Airborne abrasion treatment to the specimens showed a significant difference between the abraded groups and the control group (<0.001); further AMANNGIRRBACH specimens gave a higher value compare to the UPCERA specimens. The study also revealed that the heat treatment of the specimens gave significant value (<0.001) compared to the control group, but heat treatment following the air abrasion reduces the fracture strength of the sample than the air abraded group. CONCLUSION: Within the limitation of this study, it is concluded that, the fracture strength of Zirconia specimens treated with an airborne abrasion both on the veneering surface (50 µm silica coated Al2O3) and the cementing surface (50 µm AlO) was significantly higher than the heat treated and the control group. Airborne particle abrasion followed by the heat treatment reduces the fracture strength of the specimen than that ofthe group treated only by the air abrasives. The fracture strength of a commercially available Ceramill (AMANNGIRRBACH) is greater than that of a Zirconia from ZR-White (UPCERA) variety.
Passionflower Extract Induces High-amplitude Rhythms without Phase Shifts in the Expression of Several Circadian Clock Genes and .
Toda K, Hitoe S, Takeda S … +2 more , Shimizu N, Shimoda H
Int J Biomed Sci · 2017 Jun · PMID 28824345
Circadian rhythms play key roles in the regulation of physiological and behavioral systems including wake-sleep cycles. We evaluated the effects of passionflower (aerial parts of ) extract (PFE) on circadian rhythms usin...
Circadian rhythms play key roles in the regulation of physiological and behavioral systems including wake-sleep cycles. We evaluated the effects of passionflower (aerial parts of ) extract (PFE) on circadian rhythms using NIH3T3 cells and mice. PFE (100 μg/mL) induced high-amplitude rhythms in the expression of period circadian protein (Per) 2, cryptochrome (Cry) 1, superoxide dismutase (SOD) 1, and glutathione peroxidase (GPx) from 12 h after a treatment with serum-rich medium. Isovitexin 2"--glucoside, isoschaftoside, and homoorientin, which were purified from PFE, also significantly enhanced Per2 mRNA expression at 20 h. An oral treatment with PFE (100 mg/kg/day) at zeitgeber time (ZT) 0 h for 15 days improved sleep latencies and sleeping times in the pentobarbital-induced sleep test in mice, similar to muscimol (0.2 mg/kg, ). PFE induced high-amplitude rhythms without obvious phase shifts in serum corticosterone levels and the expression of Per1, Per2, and Cry1 in the liver as well as NIH3T3 cells. However, in the cerebrum, PFE enhanced the circadian expression of brain-muscle ARNT-like protein (Bmal) 1, circadian locomotor output cycles kaput (Clock), and Per1. Regarding this difference, we suggest the involvement of several neurotransmitters that influence the circadian rhythm. Indeed, PFE significantly increased dopamine levels at ZT 18 h, and then affected the mRNA expression of the synthetic and metabolic enzymes such as monoamine oxidase (MAO), catechol--methyltransferase (COMT), and glutamic acid decarboxylase (GAD). The results obtained show that PFE positively modulates circadian rhythms by inducing high-amplitude rhythms in the expression of several circadian clock genes.
Lack of Association between Mannose-binding Lectin 2 Codons 54 and 57 Gene Polymorphisms and Cervicovaginal Infections in Mexican Women.
Velazquez-Hernandez N, Aguilar-Duran M, Perez-Alamos AR … +6 more , Estrada-Martinez S, Salas-Pacheco JM, Sanchez-Anguiano LF, Sandoval-Carrillo AA, Lazalde-Medina B, Alvarado-Esquivel C
Int J Biomed Sci · 2017 Jun · PMID 28824344
The mannose-binding lectin (MBL) 2 gene has an important function in the innate immune response and activation of the third pathway of the complement system. Some studies have assessed the association of the MBL2 gene po...
The mannose-binding lectin (MBL) 2 gene has an important function in the innate immune response and activation of the third pathway of the complement system. Some studies have assessed the association of the MBL2 gene polymorphisms with cervicovaginal infections (CVI); however, there is no information about this association in Mexican women. This study aimed to determine the association between the MBL2 codons 54 and 57 gene polymorphisms with CVI in a sample of Mexican women. Through a cross-sectional study, blood samples and cervicovaginal cultures were obtain from 354 women. MBL2 genotyping was performed by real-time polymerase chain reaction with Taqman probes. Of the 354 women studied, 128 (36.2%) had CVI and 226 (63.8%) were healthy. The frequencies of the C and T variants in codon 54 in women with CVI were 83% and 17%, respectively; whereas the frequencies of these variants in healthy women were 82% and 18%, respectively. The frequencies of variants C/C, C/T, and T/T in women with CVI were 68%, 31%, and 1%, respectively; whereas the frequencies of these variants in healthy women were 68%, 29%, and 3%, respectively. With respect to codon 57, the frequencies of variants C and T were identical in women with CVI and in healthy women (97% and 3%, respectively). The frequencies of variants C/C, C/T, and T/T were identical in women with CVI and in healthy women (94%, 6%, and 0%, respectively). We conclude that MBL2 codons 54 and 57 gene polymorphisms do not associate with CVI in Mexican women.
Lack of Serological and Molecular Association between Exposure and Obesity: A Case-Control Study.
Alvarado-Esquivel C, Maldonado-Soto E, Sanchez-Anguiano LF … +8 more , Hernandez-Tinoco J, Ramos-Nevarez A, Cerrillo-Soto SM, Sandoval-Carrilo AA, Salas-Pacheco JM, Antuna-Salcido EI, Estrada-Martinez S, Guido-Arreola CA
Int J Biomed Sci · 2017 Jun · PMID 28824343
The association between infection and obesity has been scantly studied. Through an age-, and gender-matched case-control study, we determined the association of infection and obesity using serological and molecular met...
The association between infection and obesity has been scantly studied. Through an age-, and gender-matched case-control study, we determined the association of infection and obesity using serological and molecular methods. Cases included 203 persons with obesity, and controls included 203 persons without obesity. Participants were tested for the presence of anti- IgG antibodies using an enzyme-linked immunoassay (EIA). IgG seropositive individuals were further tested for the presence of anti- IgM antibodies using an EIA, and DNA by polymerase chain reaction (PCR). Anti- IgG antibodies were found in 16 (7.9%) of the 203 cases and in 18 (8.9%) of the 203 controls (OR=0.87; 95% CI: 0.43-1.77; =0.72). One (6.3%) of the 16 anti- IgG seropositive cases and 6 (33.3%) of the 18 anti- IgG seropositive controls were positive for IgM (=0.09). Mean body mass index (35.5 ± 4.5) in seropositive cases was similar (=0.57) to that (36.1 ± 4.5) found in seronegative cases. Stratification by obesity classes (I, II, and III) did not reveal differences (>0.05) in seroprevalences (7.8%, 7.9%, and 8.1%, respectively) or high (>150 IU/ml) IgG antibody levels (3.3%, 3.9%, and 2.7%, respectively). PCR was positive in 5 (31.3%) of 16 cases, and in 5 (27.8%) of 18 controls examined (=1.0). We found no serological or molecular evidence of an association between infection and obesity in people attending a public health center in the northern Mexican city of Durango.
Peruvian Maca () - III: The Effects of Cultivation Altitude on Phytochemical and Genetic Differences in the Four Prime Maca Phenotypes.
Meissner HO, Mscisz A, Baraniak M … +4 more , Piatkowska E, Pisulewski P, Mrozikiewicz M, Bobkiewicz-Kozlowska T
Int J Biomed Sci · 2017 Jun · PMID 28824342
In two trials, dietary and Glucosinolates' characteristics in four Maca phenotypes have been examined with an extension into the determination of DNA sequences. Hypocotyls of the four prime phenotypes of Peruvian Maca -...
In two trials, dietary and Glucosinolates' characteristics in four Maca phenotypes have been examined with an extension into the determination of DNA sequences. Hypocotyls of the four prime phenotypes of Peruvian Maca - Chacon, labelled as "Yellow", "Black", "Red" and "Purple" were separated from mixed Maca crops cultivated in four geographically-distant locations in the Peruvian Andes at altitudes between 2,800m and 4,300 m a.s.l. It was found that at higher altitudes where Red and Purple Maca phenotypes were grown, the significantly higher (<0.05) Glucosinolates' concentrations, adopted as the marker of Maca physiological activity, were observed with the Purple phenotype showing the highest Glucosinolates' content at 4,300m a.s.l., followed by the Red-coloured hypocotyls. Black Maca showed a reversal, but also a significant (<0.05) trend, while the Yellow phenotype showed no visible altitude-inflicted response (>0.05) and has consistently the lowest Glucosinolates content. Thus, it is reasonable to assume that the altitude at which Red, Purple and Black phenotypes of are grown, may be responsible for the variation in physiologic functionalities, leading to different than expected specific therapeutic and health benefits induced by Maca phenotypes grown at diverse altitudes. Although promising, insufficiently precise differences in DNA sequences failed to distinguish, without any reasonable doubt, four Maca phenotypes cultivated either in the same or geographically-distant locations, and harvested at different altitudes a.s.l. Further research on DNA sequences is needed, with more primers and larger number of Maca phenotypes, considering biosynthesis of secondary metabolites and adaptation pathways induced by harsh environment at altitudes where Maca is cultivated.
Molecular Mechanisms and Biological Functions of siRNA.
Dana H, Chalbatani GM, Mahmoodzadeh H … +11 more , Karimloo R, Rezaiean O, Moradzadeh A, Mehmandoost N, Moazzen F, Mazraeh A, Marmari V, Ebrahimi M, Rashno MM, Abadi SJ, Gharagouzlo E
Int J Biomed Sci · 2017 Jun · PMID 28824341
One of the most important advances in biology has been the discovery that siRNA (small interfering RNA) is able to regulate the expression of genes, by a phenomenon known as RNAi (RNA interference). The discovery of RNAi...
One of the most important advances in biology has been the discovery that siRNA (small interfering RNA) is able to regulate the expression of genes, by a phenomenon known as RNAi (RNA interference). The discovery of RNAi, first in plants and and later in mammalian cells, led to the emergence of a transformative view in biomedical research. siRNA has gained attention as a potential therapeutic reagent due to its ability to inhibit specific genes in many genetic diseases. siRNAs can be used as tools to study single gene function both in vivo and in-vitro and are an attractive new class of therapeutics, especially against undruggable targets for the treatment of cancer and other diseases. The siRNA delivery systems are categorized as non-viral and viral delivery systems. The non-viral delivery system includes polymers; Lipids; peptides etc. are the widely studied delivery systems for siRNA. Effective pharmacological use of siRNA requires 'carriers' that can deliver the siRNA to its intended site of action. The carriers assemble the siRNA into supramolecular complexes that display functional properties during the delivery process.
Telescopic Overdenture: A Case Report.
Shruthi CS, Poojya R, Ram S … +1 more , Anupama
Int J Biomed Sci · 2017 Mar · PMID 28533736
PATIENT: This report describes the case of a 68 year old female patient who presented with the chief complaint of difficulty in chewing and poor aesthetics due to missing teeth. The patient was interested in saving the r...
PATIENT: This report describes the case of a 68 year old female patient who presented with the chief complaint of difficulty in chewing and poor aesthetics due to missing teeth. The patient was interested in saving the remaining natural teeth and desired minimal tissue coverage from the prosthesis. After consideration of all the factors involved, it was deemed advisable to resort to a palate free maxillary telescopic complete denture and a mandibular removable partial denture. DISCUSSION: Considering the age of the patient and the cost involved, implant supported prosthesis was ruled out as a treatment option for the patient. A telescopic denture was chosen as a favourable treatment option since it overcomes many of the problems posed by conventional complete dentures like progressive bone loss, lower stability and retention, loss of periodontal proprioception and low masticatory efficiency. It also provides minimal tissue coverage and better distribution of forces. Evaluation of occlusion, esthetics, phonetics and comfort after 24 hours, 1 week and 1 month of treatment showed that the patient was happy with the prosthesis and was able to speak and chew well. CONCLUSION: Telescopic overdentures have better retention and stability as compared to conventional complete dentures. They improve the chewing efficiency, patient comfort and also decrease the alveolar bone resorption. As such they are an excellent alternative to conventional complete denture treatment.
ESM-1 siRNA Knockdown Decreased Migration and Expression of CXCL3 in Prostate Cancer Cells.
Rebollo J, Geliebter J, Reyes N
Int J Biomed Sci · 2017 Mar · PMID 28533735
Endothelial cell-specific molecule-1 (ESM-1), also known as endocan, is a soluble proteoglycan expressed by the vascular endothelium, which also circulates in the bloodstream. Inflammatory cytokines and proangiogenic gro...
Endothelial cell-specific molecule-1 (ESM-1), also known as endocan, is a soluble proteoglycan expressed by the vascular endothelium, which also circulates in the bloodstream. Inflammatory cytokines and proangiogenic growth factors increase its expression, and increased serum levels have been reported in several cancer types and immunocompetent patients with sepsis. The aim of this study was to analyze the expression profile of CXC-chemokines and the effects of ESM-1 gene knockdown in proliferation, migration and CXC-chemokine expression in highly metastatic human prostate PC-3 cells. Expression profiles of CXC-chemokines were analyzed in metastatic PC-3 and non-tumorigenic PWR-1E cells. siRNA-mediated knockdown of ESM-1 was performed into PC-3 cells, which were subsequently tested for cell migration and proliferation. Effect of siRNA transfection on CXC-chemokine expression was further quantified at the transcript and protein level. RT-qPCR analysis and sandwich ELISA assay revealed higher levels of ESM-1 and several CXC-chemokines in metastatic PC-3 cells compared to non-tumorigenic PWR-1E. Transfection of PC-3 cells with ESM-1-siRNA decreased cell migration with no effect on proliferation, and it was accompanied by decrease in the transcript and protein levels of the angiogenic chemokine CXCL3. We report here for the first time the ESM-1 targeting in PC-3 cells, which resulted in decreased migration, which may be related, at least in part, to decreased expression of the angiogenic CXCL3 chemokine, whose expression was found to be reduced in ESM-1-siRNA transfected cells. Additional studies are required to ascertain the biological role of ESM-1 in prostate cancer cells and the link with the expression of CXCL3.
Gene Expression Profiling associated with Hepatoxicity in Pregnant Rats treated with Ubi Gadong () Extract.
Faradianna Lokman E, Muhammad H, Awang N … +3 more , Hasyima Omar M, Mansor F, Saparuddin F
Int J Biomed Sci · 2017 Mar · PMID 28533734
() is the most well-known starchy tuber in Malaysia and called 'ubi gadong'. Despite concerns over toxicity effects, the tuber is known to possess therapeutic values due to the presence of bioactive compounds such as sap...
() is the most well-known starchy tuber in Malaysia and called 'ubi gadong'. Despite concerns over toxicity effects, the tuber is known to possess therapeutic values due to the presence of bioactive compounds such as saponins. This study was performed to identify the changes in gene expression profiles associated with hepatoxicity in pregnant rat treated with using RT² Profiler PCR Array. The identification of steroidal saponins from was carried out by UHPLC/MS method. Treatment of caused mortality when dosage above 2000 mg/kg b.w. was given to pregnant rats. The PCR array showed that several genes were significantly up and down-regulated upon treatment with . Treatment of at 2000 mg/kg b.w leads to significant upregulation of several genes such as Btg2, Gsr, L2hgdn, S100a8, Slc17a3, Bhmt, Cd68, Cyp1a2 whereas several genes were downregulated such as Abcb1a, Aldoa, Cdc14b, Icam1, Krt18, Hpn and Maob. The consumption of extract when taken at lower dosage of 2000 mg/kg may not be harmful to rats. extract given at the highest dosage to pregnant rats caused alterations of several genes categorized in different hepatotoxic group functions such as necrosis, cholestasis and phospholipodisis.
Tobacco Smoke Exposure Reduces Paraoxonase Activity in a Murine Model.
Reed RM, Borgan SM, Eberlein M … +5 more , Goldklang M, Lewis J, Miller M, Navab M, Kim BS
Int J Biomed Sci · 2017 Mar · PMID 28533733
AIM: To demonstrate a direct inhibitory effect of cigarette smoke exposure on paraoxonase 1 activity in a murine model. METHODS: At 8 weeks old, we randomized 10 C57/bl6 mice to an environment consisting of either filte...
AIM: To demonstrate a direct inhibitory effect of cigarette smoke exposure on paraoxonase 1 activity in a murine model. METHODS: At 8 weeks old, we randomized 10 C57/bl6 mice to an environment consisting of either filtered air or cigarette smoke for 6 months. Smoke exposure (7 hours per day, 5 days per week) was standardized using a model TE-10 smoking machine and adjusted to maintain constant sidestream and mainstream smoke. After 6 months of exposure, we assessed differences in lung air space, cholesterol, lipid, and lipoprotein profiles, as well as paraoxonase activity in mice exposed to cigarette smoke extract compared to unexposed control mice. RESULTS: Cigarette smoke exposure by the protocol used was sufficient to result in pathologic changes in lung architecture consistent with emphysema. Specifically, we observed that mice exposed to cigarette smoke had a significantly higher mean linear chord length compared to animals that were exposed to filtered air (<0.02). Despite this exposure, no differences in total HDL-cholesterol levels or HDL-cholesterol sub-fractions (i.e. HDL2 and HDL3 fractions) were noted between smoke-exposed and unexposed animals (=1.00, 0.6, and 0.4, respectively). Notably, mean HDL-cholesterol levels were identical between groups (92.8 vs 92.8 mg/dL, =1.0). Paraoxonase activity, however, was markedly reduced in mice exposed to cigarette smoke compared to those who were not exposed (102, SD=9.6 vs 144, SD=4.1 units of activity, respectively, =0.002). CONCLUSION: In this murine model, tobacco smoke exposure directly inhibits paraoxonase activity independently of HDL-cholesterol levels rather than indirectly via reduction in HDL-cholesterol levels.
E-Cadherin and FGFR1 Expression in Mouse Osteoblastogenesis in Normoxic Cultures.
Al-Amer O
Int J Biomed Sci · 2017 Mar · PMID 28533732
E-cadherin is a cell surface adhesion molecules that play an important role in tissue differentiation. FGFR1 is expressed in the developing and mature skeleton in patterns suggestive of both unique and redundant function...
E-cadherin is a cell surface adhesion molecules that play an important role in tissue differentiation. FGFR1 is expressed in the developing and mature skeleton in patterns suggestive of both unique and redundant function. Expression levels of E-cadherin and FGFR1 during osteoblastogenesis unclear. In this study primary calvarial mouse osteoblasts were differentiated to mature osteoblasts in osteogenic medium. Alkaline phosphatase (ALP) activity, alizarin red staining, gene expression (Runt-related transcription factor 2 (Runx2), collagen 1 (COL1A2), osteocalcin, E-cadherin and FGFR1) and protein expression (E-cadherin and FGFR1) of osteogenic-cultured primary mouse osteoblast were analysed in this study. The osteogenesis capacity of primary osteoblasts was significantly promoted as ALP activity, alizarin red staining, and the relative expression of Runx2 mRNA and COL1A2 mRNA significantly increased during osteoblastogenesis. The results demonstrated that E-cadherin mRNA and protein were expressed in immature osteoblasts (day 7), but not in mature osteoblasts (day 28). In contrast, the expression of FGFR1 mRNA and protein significantly highly expressed in mature osteoblasts (day 28) compared with immature osteoblasts (day 7). In conclusion, this study demonstrated that E-cadherin could be used as a marker for immature osteoblasts, whereas FGFR1 could be used as a marker for mature osteoblasts during osteoblastogenesis.
Effect of Loss of Teeth and its Association with General Quality of Life using Geriatric Oral Health Assessment Index (Gohai) among Older Individuals Residing in Rural Areas.
Kundapur V, Hegde R, Shetty M … +3 more , Mankar S, Hilal M, Prasad A H
Int J Biomed Sci · 2017 Mar · PMID 28533731
The development of measures for assessing oral health status is essential to the evolution and maturation of a scientific knowledge based in geriatric dentistry. Their development as branches of clinical care depends in...
The development of measures for assessing oral health status is essential to the evolution and maturation of a scientific knowledge based in geriatric dentistry. Their development as branches of clinical care depends in part as ability to demonstrate an effective grasp of the problems of elderly and their solutions. Any strategy for altering the health status of elderly requires a technology for first assessing that health status and then detecting increments of progress. Development of geriatric oral health assessment index (GOHAI) is a self reported measure designed to assess the oral health problems of older individuals. The goal of geriatric assessment is to collect information that will facilitate diagnosis and suggest appropriate interventions. Such a measure would facilitate estimation of prevalence of oral functional problems in older individuals, It would also increase understanding of psychological impact of oral disease and provide a means for comparing the effectiveness of various dental treatment. It also emphasizes on social well being and reflects quality of life following replacement of missing teeth.
Forensic Odontology: The New Dimension in Dental Analysis.
Divakar KP
Int J Biomed Sci · 2017 Mar · PMID 28533730
Forensic Odontology a branch of Forensic sciences uses the skill of the dentist in personal identification during mass calamities, sexual assault and child abuse to name a few. This branch not stranger to many has been g...
Forensic Odontology a branch of Forensic sciences uses the skill of the dentist in personal identification during mass calamities, sexual assault and child abuse to name a few. This branch not stranger to many has been growing tenfold in its potential and its ability to bring the forlorn to justice where a dental remains is the only available evidence. It's role and importance in the judiciary is fast growing and hence in depth knowledge in this field seems more than justified.
A Comparison between the Tie-over and Closed Suction Drainage Therapeutic Strategies in Patients Suffering from Sacral Pilonidal Sinus.
Ahmadinejad M, Ahmadi K, Ahmadinejad I … +2 more , Masoud Hashemian A, Khademhoseini P
Int J Biomed Sci · 2016 Dec · PMID 28190985
BACKGROUND AND OBJECTIVES: Pilonidal sinus is a disease in the sacrococcygeal region diagnosed through the purulent discharges of the above-said region. Although the exact pathology and etiology of those suffering from p...
BACKGROUND AND OBJECTIVES: Pilonidal sinus is a disease in the sacrococcygeal region diagnosed through the purulent discharges of the above-said region. Although the exact pathology and etiology of those suffering from pilonidal is not clear yet, the presence of hair seemingly plays a major role in the process of infection and the granulation tissue. Several techniques have been identified for pilonidal surgery. These techniques primarily fall within two categories: Primary repair and Lay open. One of the setbacks of the primary repair method is the creation of a dead area under the wound which can result in blood accumulation and seroma. To solve the problem of removing the dead space, there are two solutions. The first method utilizes a close suction drain, when the wound discharges are over, the drain is removed. The second technique is called Tie-over where different layers of the wound are pushed close to one another and the dead region vanishes. The present research seeks to compare Tie-over and Closed Suction Drainage methods through random clinical trial in order to introduce the superior technique for faster recovery and reduction of the economic load on the patient. METHODS: Some 64 patients suffering from sacral plonidal sinus aging from 15 to 50 in Shohadaye Ashayer Hospital of Khoram Abad in 2013 were selected for the research based on the inclusion criteria. The demographic information of them was collected through questionnaires. The patients were randomly divided into two groups undergoing (A) Tiover and (B) Closed Suction Drainage surgeries. Other information such as return to normal activity and total recovery time was also completed through the questionnaire. SPSS software was used to conduct statistical analysis. RESULTS: The results of the statistical analysis showed that the two groups were similar to one another in terms of age, gender, marital status, job, and literacy. A significant difference was observed only between the level of satisfaction (-value = 0.035) in groups A and B concerning the factors studied after the operation. The post-operation recurrence of pilonidal sinus among those who smoked cigarettes and had undergone Closed Suction Drainage was significantly greater than the non-smokers (-value=0.011). As of the group undergoing Tie-over surgery, the difference between the patients' satisfaction in terms of their age was statistically significant and the highest level of satisfaction was observed among those aging 25 to 34 (90%) (-value=0.023). CONCLUSION: In sacrococcygeal pilonidal cyst surgery, no difference was observed except for the difference in the level of satisfaction. More satisfaction was observed using the Tiover method.
Study of High Sensitive C-Reactive Protein (HS-CRP) After Cardiac Rehabilitation Program in Patients Undergoing Isolated CABG.
Johari Moghadam A, Azizinejad S
Int J Biomed Sci · 2016 Dec · PMID 28190984
INTRODUCTION: Although cardiac rehabilitation is known as a tool to reduce the overall risk of cardiovascular complications, its specific role in the reduction of hs-CRP as a marker of inflammation and a proven marker of...
INTRODUCTION: Although cardiac rehabilitation is known as a tool to reduce the overall risk of cardiovascular complications, its specific role in the reduction of hs-CRP as a marker of inflammation and a proven marker of cardiovascular risk needs further investigation. AIM: The present study aims at elucidating the effects of a full course of conventional cardiac rehabilitation program for the period of eight weeks, on the levels of hs-CRP in patients who underwent isolated coronary artery bypass surgery. MATERIAL AND METHODS: In this case study, 30 consecutive patients who underwent isolated coronary artery bypass surgery (isolated CABGS), and a full 8-week cardiac rehabilitation program in Tehran Heart Center, were investigated. A group of 30 similar patients, who enrolled in the same period of rehabilitation program but did not participate in practice, was considered as a control group. Serum levels of hs-CRP in both groups were measured retrospectively and in similar days before the start of rehabilitation program and at the end of it (or 8 weeks after initial registration for the control group). RESULTS: Levels of hs-CRP in the rehabilitation group and control group were 5.9 7.7 and 6.3 6.9 respectively before start of the program which was not statistically meaningful (-Value = 0.833). However, after the program, level of hs-CRP in the two tested groups changed to 2.3 5.1 and 5.7 6.1 respectively which showed a meaningful correlation (-Value = 0.023). These results also showed that decrease in hs-CRP level in the rehabilitated group but not in the control group was statistically meaningful (with -Value of 0.037 and 0.0723 respectively). CONCLUSION: In patients undergoing coronary bypass surgery, participating in a full course of cardiac rehabilitation for 8 weeks has resulted in a significant reduction in hs-CRP levels as a marker of cardiovascular risk.
Evaluate the Effect of Commercially Available Denture Cleansers on Surface Hardness and Roughness of Denture Liners at Various Time Intervals.
Mohammed HS, Singh S, Hari PA … +4 more , Amarnath GS, Kundapur V, Pasha N, Anand M
Int J Biomed Sci · 2016 Dec · PMID 28190983
BACKGROUND AND OBJECTIVE: Chemical cleansing by denture cleansers is first choice for denture plaque control. The most common problems while using denture cleansers are hardening, porosity, odor sorption, water sorption,...
BACKGROUND AND OBJECTIVE: Chemical cleansing by denture cleansers is first choice for denture plaque control. The most common problems while using denture cleansers are hardening, porosity, odor sorption, water sorption, solubility, and colour change, bacterial and fungal growth. Chemical cleansing procedures have been found to have an effect on the physical and mechanical properties of denture liners. Thus, this study was conducted to evaluate the effect of commercially available denture cleansers on surface hardness and roughness of acrylic and silicon based denture liners at various time interval. METHOD: Two autopolymerising denture liners Kooliner (acrylic) and GC reline soft (silicon) were tested with two commercially available denture cleansers, polident and efferdent plus. Total of 120 specimens were prepared and all the specimens were divided into six groups based on the relining materials and denture cleansers used. Surface hardness and surface roughness was tested using Shore A durometer and profilometer respectively at the end of day 1, day 7, day 30 and day 90. All the specimens were stored in artificial saliva throughout the study. Cleanser solution was prepared daily by adding Polident and Efferdent plus denture cleanser tablet into 250ml of enough very warm (not hot) water. Acrylic and silicon liner groups were cleansed in a solution of denture cleanser and water for 15 minutes daily, rinsed with water and stored in artificial saliva at room temperature. The data was analyzed with one way ANOVA and independent t-test. RESULT: The acrylic soft lining showed gradual hardening and increase in surface roughness after immersion in denture cleanser and also with time. Acrylic liner material showed maximum hardness and roughness with Polident followed by Efferdent plus and water (control group). Silicone lining material showed a slight difference in hardness and roughness between the test group and control group. There was a slight increase in hardness in all the groups with time. Very slight increase in mean surface roughness of all the silicon liner groups from day 1 to day 90 was observed. A statistically significant change was noted between and within the all silicon liner groups on day 7, day 30 and day 90. CONCLUSION: The average surface hardness and surface roughness were lower in silicon liner material than acrylic liner material. Maximum surface roughness was noted by Polident followed by Efferdent Plus and Water for both acrylic liner group and silicon liner group.
Dermatomyositis Leading to Necrotizing Vasculitis: A Perfect Response to Applied Therapy.
Akbaryan M, Darabi F, Soltani Z
Int J Biomed Sci · 2016 Dec · PMID 28190982
Dermatomyositis is an idiopathic inflammatory myopathy that cause skin and muscle complications. The ethiology is not understood well yet. Released cytokines including interferon and interleukins are suggested to make in...
Dermatomyositis is an idiopathic inflammatory myopathy that cause skin and muscle complications. The ethiology is not understood well yet. Released cytokines including interferon and interleukins are suggested to make inflammatory responses in the skin or muscle. Muscle weakness and skin lesions including heliotrope rash, shawl sign and Gottron's papules are the most common symptoms. A biopsy (muscle or skin) is always the most reliable method for diagnosis. Corticosteroids in association with immunosuppressive agents are used as standard treatment. The patient was a 30 years old woman who got involved with dermatomyositis for 10 years. She has been under therapy with Methotrexate, Prednisolon and Azathioprine until she came to us suffering from progressive skin lesions. Experiments and examinations were normal except the lesions and detected lipoatrophy. Because of immune cells infiltration and observations necrotizing vasculitis was diagnosed. After three month of high dose prednisolon and intravenous cyclophosphamide therapy the lesions vanished remarkable. True and immediate diagnosis gives physicians the chance not only to assess the best treatment but have adequate time to apply the procedure. However shortening the therapy and diminishing morbidity of the disease need more investigations and efforts.
About
- Frequency
- Sun
- Papers found
- 200
- RSS feed
- Subscribe