Nigerian Journal Of Physiological Sciences[JOURNAL]
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200 papers
Phantom Limb Neuroplasticity and Maladaptive Sensory Perceptions: Why Phantom Limb Paresthesia Should Be Integrated into Referred Pain Discussions.
Niladri Kumar Mahato
Niger J Physiol Sci · 2023 Dec · PMID 38696692
The concept of referred pain is an integral part of the anatomy didactic content taught and discussed in all medical school curricula. However, this discussion has excluded the topic of phantom limb pain, despite the ex...
The concept of referred pain is an integral part of the anatomy didactic content taught and discussed in all medical school curricula. However, this discussion has excluded the topic of phantom limb pain, despite the existence of parallels in neurophysiological explanations between these conditions. This brief viewpoint attempts to reason why phantom limb paresthesia or pain should be included in the fold of refereed pain discussions.
Cerebellar and Olfactory Bulb Perturbations Induced by Vanadium Neurotoxicity in the African Giant Rat (Cricetomys gambianus, Waterhouse).
Mustapha O, Omojola F, Olaolorun F … +1 more , Olude M
Niger J Physiol Sci · 2023 Dec · PMID 38696691 · Publisher ↗
The African giant rat, AGR (Cricetomys gambianus) is a unique rodent known for its keen sense of smell which has enabled its use in the diagnosis of tuberculosis and demining activities in war torn countries. This keen s...
The African giant rat, AGR (Cricetomys gambianus) is a unique rodent known for its keen sense of smell which has enabled its use in the diagnosis of tuberculosis and demining activities in war torn countries. This keen sense of smell and the ability to navigate tight spaces are skills modulated by the olfactory bulb and cerebellum. While the brain is generally susceptible to environmental pollutants such as heavy metals, vanadium has predilection for these two brain regions. This work was thus designed to investigate the probable neurotoxic effect of vanadium on the neuronal cytoarchitecture of the cerebellum and olfactory bulb in this rodent. To achieve this, twelve adults male AGRs were divided into two groups (vanadium and control groups) and were given intraperitoneal injections of 3mg/kg body weight sodium metavanadate and normal saline respectively for 14 days. After which they were sacrificed, and brains harvested for histological investigations using Nissl and Golgi staining techniques. Results from our experiment revealed Purkinje cell degeneration and pyknosis as revealed by a lower intact-pyknotic cell (I-P) ratio, higher pyknotic Purkinje cell density and poor dendritic arborizations in the molecular layer of the cerebellum in the vanadium treated group. In the olfactory bulb, neuronal loss in the glomerular layer was observed as shrunken glomeruli. These neuronal changes have been linked to deficits in motor function and disruption of odor transduction in the olfactory bulb. This work has further demonstrated the neurotoxic effects of vanadium on the cerebellum and olfactory bulb of the AGR and the likely threat it may pose to the translational potentials of this rodent. We therefore propose the use of this rodent as a suitable model for better understanding vanadium induced olfactory and cerebellar dysfunctions.
Ameliorative Effects of Coconut Water on Hematological and Lipid Profiles of Phenylhydrazine-treated Rats.
Adeleye GS, Odesanmi EO, Ajeigbe KO … +3 more , Omayone T, Odetola A, Sobanke AO
Niger J Physiol Sci · 2023 Dec · PMID 38696690 · Publisher ↗
Anaemia is a widespread health issue affecting young children and pregnant women, characterized by reduced red blood cells or haemoglobin levels. Coconut water, rich in nutrients such as L-arginine, iron, vitamin C, vita...
Anaemia is a widespread health issue affecting young children and pregnant women, characterized by reduced red blood cells or haemoglobin levels. Coconut water, rich in nutrients such as L-arginine, iron, vitamin C, vitamin B6, folic acid and fatty acids, is believed to aid in blood formation (hematopoesis). The study aimed to examine the impact of coconut water on hematological indices and lipid profiles in rats with phenylhydrazine-induced anemia. 30 rats were divided into 5 groups: a normal control, phenylhydrazine untreated, coconut water (0.5ml/kg), iron, and ferrous treated groups. Hemoglobin, hematocrit, and erythrocyte levels were measured using a Hematology Analyzer. Results showed a significant decrease in LDL and TG levels, and an increase in HDL levels in phenylhydrazine induced anemia compared to the control group. Coconut water administration at 0.5ml/kg reduced LDL, VLDL and TG levels, and increased HDL levels in rats with induced anemia. The study found that coconut water had a positive effect on hematological indices, as it increased hemoglobin and erythrocyte levels in rats with induced anemia. These findings suggest that coconut water may have potential therapeutic benefits for individuals with anemia, particularly in lowering lipid levels and improving blood formation. However, further research is needed to fully understand the mechanisms underlying these effects and to determine the most effective dosage and duration of treatment. Overall, the study highlights the importance of coconut water as a potentially beneficial alternative treatment for anemia.
Effect of Acute Caffeine Exposure on Blood Glucose and Hepatic Glycogen Content in Normal and Thyroidectomized Male Wistar Rats.
Shittu ST, Isehunwa GO, Alada AA
Niger J Physiol Sci · 2023 Dec · PMID 38696689 · Publisher ↗
Acute caffeine exposure had been shown to induce hyperglycemia however; the influence of thyroid hormones on the caffeine-induced hyperglycemia is yet to be established. The present study was therefore designed to invest...
Acute caffeine exposure had been shown to induce hyperglycemia however; the influence of thyroid hormones on the caffeine-induced hyperglycemia is yet to be established. The present study was therefore designed to investigate the effect of caffeine exposure on blood glucose and hepatic glycogen content in thyroidectomized rats. Sixty adult male Wistar rats were randomly divided into 10 groups as I-X (n=6). Rats in groups I, III, V, VII and IX were given normal saline, caffeine, prazosin + caffeine, propranolol +caffeine, combined prazosin+ propranolol+caffeine injections respectively while rats in groups II, IV, VI, VIII and X were thyroidectomized and treated in similar manner as the normal rats respectively. Surgical removal of the thyroid gland was done in the thyroidectomised groups while sham-operation was done in Normal group to serve as control. After healing and following an overnight fast, the rats were anaesthetized and the femoral vein and carotid artery were cannulated for drug administration and blood glucose measurement respectively. After stabilization, following basal measurements, rats from each group were injected normal saline or caffeine (6mg/kg) while another sets were pre-treated prazosin (0.2 mg/kg), propanolol (0.5 mg/kg) or their combination before caffeine administration. Blood glucose was then monitored for 60 minutes post-injection of caffeine at 5 minutes interval. Liver samples were collected at the end of the observation period for glycogen content determination. Caffeine caused significant increased blood glucose levels in both normal and thyroidectomized rats which were up to 210% and 180% respectively at the peak of their responses. Liver glycogen content of the thyroidectomized rats (3.11 ± 0.20 mg/100g tissue weight) was significantly higher than the normal rats (1.91 ± 0.43 mg/100g tissue weight). These glycogen contents were significantly reduced by caffeine in both normal (0.25 ± 0.04 mg/100g tissue weight) and thyroidectomized rats (1.65 ± 0.16 mg/100g tissue weight) when compared with their controls. The caffeine effects on blood glucose and hepatic glycogen content were abolished by pretreatment with propanolol or a combination of prazosin and propanolol in both normal and thyroidectomized rats but pretreatment with prazosin caused only significant reduction in hyperglycemic response to caffeine. The findings of this study suggest that caffeine-induced hyperglycemia in both normal and thyroidectomized rats are mediated through both alpha and beta adrenoceptors.
Brain antioxidant status and gene expressions of nicotinic and dopamine receptors are improved by black seed oil administration in cigarette smoke or nicotine vapour-exposed rats.
Adejare A, Oloyo AK, Ishola IO … +5 more , Busari AA, Ismail-Badmus KB, Abdulrazaq MM, Osifala OO, Salami MO
Niger J Physiol Sci · 2023 Dec · PMID 38696688 · Publisher ↗
BACKGROUND: Smoking is associated with dysregulation of the antioxidant system and addiction. AIM: This study sought to ascertain the effect of Nigella Sativa (NS) oil on the antioxidant system, nicotine/tobacco addictio...
BACKGROUND: Smoking is associated with dysregulation of the antioxidant system and addiction. AIM: This study sought to ascertain the effect of Nigella Sativa (NS) oil on the antioxidant system, nicotine/tobacco addiction as well as the expressions of α4β2 nicotinic (nAChR) and dopamine type-2 (DRD2) receptors in selected brain regions of the rat. METHODS: Thirty male Sprague-Dawley rats were divided into 6 groups comprising of vehicle-treated control, NS oil only, Smoke only, Smoke + NS oil, Nicotine only and Nicotine + NS oil. Animals were passively exposed to cigarette smoke or nicotine vapour for 12 weeks, however, NS oil treatment commenced from 9th-12th week of the experimental duration. RESULTS: Nicotine vapour and cigarette smoke-induced increase in cotinine level were significantly ameliorated by NS treatment. Cigarette smoke or nicotine vapour exposure significantly (p<0.05) decreased the level of antioxidant enzymes while increasing malondialdehyde level in the brain homogenates of the rats. Administration of NS oil significantly (p<0.05) reversed the reduced antioxidant level. Cigarette-smoke also significantly increased α4-nAChR expression in the frontal cortex and olfactory bulb compared to control. Nicotine vapour significantly increased DRD2 expression only in the olfactory cortex. NS oil administration reduced both the cigarette-smoke-induced increase in α4-nAChR and nicotine vapour-induced increase in DRD2 gene expression only in the olfactory cortex. CONCLUSION: Findings from this study suggest that NS oil improves brain antioxidant status while ameliorating nicotine vapour and cigarette smoke addiction through down-regulation of α4-nAChR and DRD2 gene expressions in discrete brain regions in Sprague-Dawley rats.
Quercetin Exerts Anticonvulsant Effect through Mitigation of Neuroinflammatory Response in Pentylenetetrazole-induced Seizure in Mice.
Bakre AG, Adeoluwa OA, Adeoluwa GO … +4 more , Adeniyi FR, Oni JO, Akinluyi ET, Olojede SO
Niger J Physiol Sci · 2023 Dec · PMID 38696687 · Publisher ↗
Epilepsy is a chronic disease of the brain characterized by seizures. The currently available anticonvulsants only treat symptoms with serious adverse drug reactions. Therefore, there is need for new therapeutic interven...
Epilepsy is a chronic disease of the brain characterized by seizures. The currently available anticonvulsants only treat symptoms with serious adverse drug reactions. Therefore, there is need for new therapeutic intervention that will prevent epileptogenesis with greater therapeutic success. Quercetin (QT) is a flavonoid with known neuroprotective and anti-inflammatory properties. The study aimed to investigate its effects against pentylenetetrazole (PTZ)-induced seizures. Animals were divided into four groups (n = 10). Group 1(control) only received vehicle (10 mL/kg), group 2 received vehicle, groups 3 and 4 received QT 12.5 mg/kg and 25 mg/kg respectively. Sixty minutes after treatments, animals in groups 2 to 4 were injected with sub-convulsive dose of pentylenetetrazole (35 mg/kg, i.p.) on every alternate day (48±2h) for 21 days. The mice were observed for 30 minutes after each PTZ injection for seizure activity. Brain samples were collected for biochemical assays. Administration of PTZ caused significant increase in the intensity of seizures, neuronal degeneration and level of proinflammatory cytokines in animals compared to control. These behavioural alterations were attenuated significantly by QT (12.5 and 25 mg/kg). The PTZ-induced increase in IL-12, TNF-α and IFN-ɣ were significantly reduced by pre-treatment with the QT (12.5 and 25 mg/kg, p.o). Quercetin also reduced neuronal loss compared to control. Quercetin attenuates seizures in kindled mice and reduces neuroinflammation and neurodegeneration. This neuroprotective effect may be attributed to its ability to inhibit inflammatory mediators in the brain.
The Use of Levels One and Two Dermatoglyphics for Sex Identification in University of Ibadan Community, Southwest Nigeria.
Adetona MO, Preyor E
Niger J Physiol Sci · 2023 Dec · PMID 38696686 · Publisher ↗
Dermatoglyphic traits are genetically determined and remain constant until death. Dermatoglyphics features are arranged from patterns, minutiae and ridgeology. This study utilized patterns and minutiae details of fingerp...
Dermatoglyphic traits are genetically determined and remain constant until death. Dermatoglyphics features are arranged from patterns, minutiae and ridgeology. This study utilized patterns and minutiae details of fingerprints as a means of sexual differentiation amongst the University of Ibadan community. Three hundred and eighty-four (192 males and 192 females) participants from the University of Ibadan community were recruited using multistage sampling technique. Fingerprints were obtained using fingerprint scanner Dermalog LF10, Hamburg, Germany. GraphPad Prism 7.0 was used for the test of mean of variables. Ulnar loop, whorl and radial loop patterns were found to be predominantly distributed in both male and female in that order. However, the arch pattern was significantly different between female and male. The male subjects had significantly higher total finger ridge count (TFRC). All the analysed minutiae were significantly different between male and female except bridge. The arch pattern, TFRC and level 2 details (minutiae) of dermatoglyphics could be used as markers for sexual differentiation.
Low-dose Potassium bromate enhances ischemia-reperfusion-induced gastric ulcer healing in Thyroidectomised Rats.
Salami AT, Chukwukaeme C, Olagoke O … +1 more , Olaleye SB
Niger J Physiol Sci · 2023 Dec · PMID 38696685 · Publisher ↗
Gastric ulcer healing is impaired in both hypothyroid and hyperthyroid conditions. Thyroid hormones regulate growth, energy metabolism and mitochondrial oxidative metabolism. Xenobiotics have been documented to negative...
Gastric ulcer healing is impaired in both hypothyroid and hyperthyroid conditions. Thyroid hormones regulate growth, energy metabolism and mitochondrial oxidative metabolism. Xenobiotics have been documented to negatively impact the thyroid gland at high doses but the redox and cellular interactions at low doses during wound healing process remains unclear. Potassium bromate has been documented to be toxic at high doses but there is dearth of information on its activities at a low dose in varied thyroid states which was evaluated in this study. 60 male Wistar rats (g, n=10) were randomised into 2 conditions: Normal, ulcerated untreated, ulcerated treated with 12.5mg/kg p.o KBrO3 and thyroidectomised groups: thyroidectomised ulcerated, thyroidectomised ulcer treated with KBrO3 and thyroidectomised treated with thyroxine (100µg/kg) Total thyroidectomy was used to model hypothyroidism, and ischaemia-reperfusion-induced gastric ulcers were monitored for healing. Daily body weights, Levels of thyroxine, Gastric mucin content, redox and sodium pump activity were examined alongside other markers of hepatic and haematological toxicity by days 3 and 7 post ulceration. Data were analysed using descriptive statistics and ANOVA α 0.05. The bromate-exposed hypothyroid rats showed increased gastric ulcer healing potential with reduced gastric epithelial oedema and inflammation; hepatic steatosis, and periportal inflammation. Haematological variables and markers of hepatic functions were normal. There were reduced levels of gastric and hepatic malondialdehyde levels. Thyroxine and potassium bromate treatment resolved the redox and cellular toxicity possibly via increasing catalase and sulfhydryl levels and increased Na+ K+ pump activity. We conclude that potassium bromate enhanced gastric ulcer healing in hypothyroid state, similar to thyroxine treatment.
Electrocardiography, Blood Pressure Measurements, Vital Parameters and Anaesthetic Indices in the African Giant Rat (Cricetomys Gambianus Waterhouse) Immobilized with Diazepam or Ketamine.
Igado O, Abiola JO, Anifowose OR … +3 more , Alaba BA, Nottidge HO, Omobowale TO
Niger J Physiol Sci · 2023 Dec · PMID 38696684 · Publisher ↗
In spite of the increasing use and importance of the African giant rat (Cricetomys gambianus Waterhouse) in research, and other fields, like location of landmines, there is still not enough information on their physiolog...
In spite of the increasing use and importance of the African giant rat (Cricetomys gambianus Waterhouse) in research, and other fields, like location of landmines, there is still not enough information on their physiology. In this study, we assessed the electrocardiogram, blood pressure, vital parameters and anaesthetic indices of the African giant rat (Cricetomys gambianus Waterhouse), both genders, using diazepam or ketamine as chemical restraints. A total of 24 adult African Giant Rats (AGR), 12 males and 12 females were used in this experiment. The animals were divided into two groups of twelve animals each (6 males and 6 females). One group was assessed for the effect of diazepam, and the other group ketamine. Diazepam (Roche®, Switzerland) was administered intraperitoneally at a dose rate of 7.5 mg/kg, while ketamine was administered intraperitoneally at a dose rate of 45 mg/kg. Parameters measured were recorded from the time desirable sedation was achieved, and every 15 minutes till the animal was awake. Animals administered diazepam took a longer time to sleep or achieve desirable sedative state, a longer time to respond to stimuli before waking up fully and a longer time to be fully awake, relative to ketamine-induced sedation. Ketamine caused a continuous increase in respiratory rate and blood pressure, while diazepam caused a continuous decrease in the respiratory rate. The electrocardiogram showed tachycardia throughout the experiment with the use of both drugs, although this was more pronounced with the use of diazepam, causing a decrease in QRS interval and a decrease in QT interval. Gender differences were observed in most parameters measured. The results obtained gave baseline values for electrocardiogram and blood pressure readings, while also detailing the changes and gender differences observed with sedation. In addition, results indicated ketamine is best used for short procedures and diazepam at a higher dose used for procedures requiring longer time in the African giant rat.
Methanolic Extract of Ricinus Communis ameliorated cardiovascular dysfunction in dichlorvos-exposed rats.
Adeoye SWA, Mayowa MF, Akano FM … +1 more , Sultan AO
Niger J Physiol Sci · 2023 Dec · PMID 38696683 · Publisher ↗
Cardiovascular diseases are the leading causes of death globally resulting in 17-19 million death every year. The search for an effective medicine to manage cardiovascular disorder without any side effect has led to the...
Cardiovascular diseases are the leading causes of death globally resulting in 17-19 million death every year. The search for an effective medicine to manage cardiovascular disorder without any side effect has led to the use of traditional based medicine. 75% of the world's population has been reported to depend on traditional medicine as their basic form of health care and this has resulted to the use of herbal medicine in the treatment and management of metabolic diseases. The study evaluated the effect of methanolic extract of Ricinus communis on DDVP-induced cardiotoxicity in male Wistar rats. Thirty-two (32) male Wistar rats were randomly divided into four groups of eight (8) rats each. Group A served as control rats, received 10mL/Kg of dimethyl sulfoxide (DMSO) and distilled water solution (vehicle) for six weeks. Group B served as DDVP-induced rats and were exposed to DDVP (15 minutes daily) for 3 weeks without any treatment. Group C rats received DDVP as in group B and then administered 300mg/kg of R. communis extract for 42days. While Group D rats were administered 300mg/kg of R. communis extract daily, for 6 weeks in addition to normal feed and water. Exposure to DDVP caused significant cardiac dysfunction evidence by alteration in cardiovascular variables and electrocardiac function, compromised lipid profile and reduced antioxidant enzymes. However, treatment with methanolic extract of Ricinus communis improved antioxidant defense system, attenuate hemodynamic impairment and left ventricular dysfunction, as well as inhibit lipid peroxidation and prevent hyperlipidemia in rats. In addition, histopathology observation showed that Ricinus communis extract was able to regenerate the myocardial injury caused by exposure to dichlorvos. In conclusion, Ricinus communis exhibited cardioprotective properties and may be a potential remedy for cardiovascular diseases with low risk of toxicity.
Morphological and morphometrical studies on the tongue of the juvenile cattle egret (Bulbucus ibis).
Olopade JO, Azeez IA, Omirinde JO … +2 more , Elisha J, Plang NJ
Niger J Physiol Sci · 2023 Dec · PMID 38696682
Cattle egret (Bubulcus ibis) is a cosmopolitan heron species, with least concern conservation status. There are limited literatures on the anatomy of this bird, especially in relation to its sensory organs, hence we here...
Cattle egret (Bubulcus ibis) is a cosmopolitan heron species, with least concern conservation status. There are limited literatures on the anatomy of this bird, especially in relation to its sensory organs, hence we here investigated the gross morphological and histomorphometric features of its tongue. The tongues of twelve healthy juvenile cattle egrets were examined in situ for morphological appearance and gross morphometric measurements were determined ex situ. Routine histology was conducted on the tongue tissue with parameters such as epithelial and lamina propia heights, lingual muscle and entoglossal cartilage heights evaluated. Grossly, the tongue was divided into three parts name; apex, body and the root. It was arrow shaped, conforming to the shape of the beak, with a laryngeal mound bounded caudally by the pharyngeal papillae at its root. A massive entoglossal cartilage formed the core of the cranial apex, ventral body portion, and caudal aspect of the root. Histologically, the lingual mucosa possessed keratinized squamous epithelium in all its divisions, with spinous conical papillae being characteristic of the cranial apical mucosa. The body lingual mucosa possessed foliate papillae on the dorsal aspects, while filiform papillae were prominent in the ventral portions. The lingual root uniquely possessed numerous glandular ducts in its lamina propia as well as localized adipocytes. Overall, the regression analysis data showed that the body weight can be conveniently predicted from tongue parameters. This study has thus provided additional knowledge on the anatomy of the birds and the generated data could prove useful in comparative regional anatomy.
Protocatechuic acid modulates hepatic oxidative stress and inflammation linked to DMN exposure in rat.
Asejeje F, Etim S, Asejeje G … +4 more , Iwuoh BC, Akintade SI, Adedara I, Farombi EO
Niger J Physiol Sci · 2023 Dec · PMID 38696681 · Publisher ↗
Dimethyl nitrosamine (DMN), a potent hepatotoxin, exerts carcinogenic effects and induces hepatic necrosis in experimental animals via CYP2E1 metabolic activation, and generation of reactive oxygen species (ROS). Protoca...
Dimethyl nitrosamine (DMN), a potent hepatotoxin, exerts carcinogenic effects and induces hepatic necrosis in experimental animals via CYP2E1 metabolic activation, and generation of reactive oxygen species (ROS). Protocatechuic acid (PCA), a plant-based simple phenolic compound and potent antioxidant, has been shown to affect the development of neoplasia in the rat liver and inhibit the initiation or progression phases of most cancers. In this study, the modulatory effects of PCA on DMN-induced hepatotoxicity, oxidative stress, inflammation, and selected phase I xenobiotic metabolizing enzymes were investigated in male Wistar rats. This study assessed biomarkers of hepatic injury (alanine transaminase, aspartate aminotransferase, alkaline phosphatase, and gamma- glutamyl transferase); oxidative stress (hydrogen peroxide concentration, lipid peroxidation, and reduced glutathione levels); measured activities of antioxidant enzymes (catalase, sodium dismutase, glutathione peroxidase, glutathione S-transferase); and inflammation (Tumor necrosis factor (TNF)-α, interleukin-1-Beta (IL-1β) and iNOS). The results of our investigation demonstrated that pretreatment with PCA at 50 and 100 mg/kg body weight p.o. reduced DMN (20 mg/kg bw) i.p. mediated hepatic injury, oxidative stress, and inflammation in a dose-dependent manner. In addition, the activities of phase I metabolizing enzymes were significantly induced except for aminopyrine-N-demethylase in the DMN-treated rats when compared with the DMN alone control group. This induction was also reversed by pre-treatment with PCA. The result of this study suggests that PCA is hepatoprotective against DMN-induced hepatic damage by its ability to suppress oxidative stress, inflammation, and modulate the activities of the selected phase I drug metabolizing enzymes. Thus, PCA may prove useful in combating DMN-induced hepatic damage.
Evaluation of Testicular Function and Structural Changes of Wistar Rats Following Antiretroviral Exposure: Protective Role of Cyperus Esculentus.
Ogedengbe OO, Bature A, Fafure AA … +5 more , Kehinde SO, Adekeye AO, Akintayo CO, Ajiboye BO, Adeeyo OA
Niger J Physiol Sci · 2023 Dec · PMID 38696680 · Publisher ↗
Long-term antiretroviral drug toxicity may exacerbate the impact of HAART-Cyperus esculentus (C. esculentus) interactions on testicular function in HIV-infected individuals. This study examined the ability of C. esculent...
Long-term antiretroviral drug toxicity may exacerbate the impact of HAART-Cyperus esculentus (C. esculentus) interactions on testicular function in HIV-infected individuals. This study examined the ability of C. esculentus plants to treat testicular dysfunction, which is thought to be a probable side effect of antiretroviral toxicity. Adult Wistar male rats weighing 90-110 g were divided into six groups and administered the prescribed treatments. In addition to testicular histology and stereological parameters, testosterone levels, follicle-stimulating hormone levels, antioxidant markers, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione levels were also evaluated. The adverse consequences of highly active antiretroviral therapy (HAART) include considerable loss of germ cells, enlargement of the tubular lumen, widening of interstitial gaps, and severe hypocellularity. Compared to the other treatment groups, MDA levels dramatically increased, whereas GSH and antioxidant enzyme (SOD) levels significantly decreased. Testicular architecture was largely conserved after treatment with C. esculentus, with a notable increase in the cellular densities of germinal and interstitial cells and a notable decrease in the tubular lumen. Vacuolation, architectural malformations, and hypoplastic changes were reduced. Significant improvements were also observed in C. esculentus in terms of elevated antioxidant SOD and GSH levels and decreased MDA levels. C. esculentus reduced architectural distortions and testicular dysfunction caused by HAART, and improved testicular morphology. Further exploration of these pathways is required.
Gestational administration of aqueous leaf extract of Jatropha tanjorensis alleviated postpartum emotional and cognitive dysfunction in rats (Wistar strain).
Antai A, Ukoh I, Sunday B … +2 more , Edet E, Johnny M
Niger J Physiol Sci · 2023 Dec · PMID 38696679 · Publisher ↗
This study examines the effect of gestational administration of aqueous leaf extract of Jatropha tanjorensis (JT) on postpartum-like behavioural outcomes to delineate its possibility as a prophylactic, therapeutic agent...
This study examines the effect of gestational administration of aqueous leaf extract of Jatropha tanjorensis (JT) on postpartum-like behavioural outcomes to delineate its possibility as a prophylactic, therapeutic agent in the treatment of postpartum symptoms. Inseminated female rats (120-150g) were grouped into two-control and JT group (n=10). Control received 20 ml/kg of distilled water and JT group received 500 mg/kg of JT orally once daily for 21 days in gestation. Non-pregnant rats were excluded from the study. Parameters assessed at postpartum include antidepressant-like (force swim test, FST; tail suspension-test, TST), locomotor (open field test, OFT), anxiolytic-like (elevated plus maze, EPM; light-dark box, LDB), learning and memory (T-maze; novel object recognition task, NORT), social (nest score) and analgesic-like (hot plate test, HPT; tail flick test, TFT) behaviours. JT increased (P<0.05) mobility and latency to immobility durations in FST and TST; open arm entry (P<0.001) and duration (P<0.01) in EPM and light box duration (P<0.05) in LDB; locomotion and exploration, but reduced anxiety-like levels in EPM, LDB and OFT. It increased nest score (P<0.05); mean retraction time (P<0.01) of TFT. JT showed positive score for short and long term memory in NORT and improved percentage alternation in T-maze though not significant compared to control. In conclusion, the aqueous extract has a therapeutic effect that reduces postpartum-like depression and anxiety, and improves locomotor activity. JT can be a preventive and adjuvant therapeutic option for pregnant women.
Antidepressant-Like Effects of Cinnamomum verum on Open-Space Forced Swim-Induced Depression in Mice.
Yusha'u Y, Aminu Hanafi A, Muhammad Adam U
Niger J Physiol Sci · 2023 Dec · PMID 38696678 · Publisher ↗
Depression is a mental disorder characterized by depressive episodes, such as low mood, low self-esteem, feeling of guilt, and poor concentration. Depression has a high comorbidity with cognitive impairments. Studies hav...
Depression is a mental disorder characterized by depressive episodes, such as low mood, low self-esteem, feeling of guilt, and poor concentration. Depression has a high comorbidity with cognitive impairments. Studies have shown that cinnamon has anti-inflammatory antiviral, antihypertensive, antioxidant and anti-diabetic potentials. Therefore, the aim of the research was to assess the antidepressant effect of cinnamon on open-space forced swim-induced depression in mice. Twenty-five (25) Swiss albino mice were grouped into five groups (n=5). Group I: control (negative control) exposed to open-space forced swim test (OSFST) without any treatment, Groups II, III and IV received graded doses of Cinnamon 12.5, 25, and 50 mg/kg, group 5 (positive control) received fluoxetine 20 mg/kg orally. The animals were subjected to OSFST, Open Field Test (Line Crossing) and Novel Object Recognition Test (NORT). Administration of cinnamon showed decreased immobility time (behavioural despair) in OSFST compared to control and fluoxetine groups (p < 0.05). However, no statistically significant effect was observed in line crossing (locomotor activity) and the discrimination ratio of NORT (non-spatial short-term memory) between cinnamon administered groups and the control group. In conclusion, cinnamon has shown antidepressant-like effect in open-space forced swim-induced depression in mice. Keywords: Cinnamon, Depression, Cognitive impairment, Immobility time, Behavioural despair.
Antioxidant and Chemopreventive Effects of Azadirachta indica on Lead Acetate-induced Hepatotoxicity in Male Wistar Rats.
Asenuga E, Ajibade TO, Adejumobi A … +6 more , Alaba B, Nabofa W, Oyagbemi A, Omobowale T, Badejo J, Akinboye O
Niger J Physiol Sci · 2022 Dec · PMID 38243572
Toxic metals such as lead (Pb) cause severe liver damage in humans and animals, with oxidative stress prominently implicated in the pathogenesis of lead acetate‑induced liver injury. Azadirachta indica is hepatoprotectiv...
Toxic metals such as lead (Pb) cause severe liver damage in humans and animals, with oxidative stress prominently implicated in the pathogenesis of lead acetate‑induced liver injury. Azadirachta indica is hepatoprotective due to its antioxidative effect. This study investigated the antioxidative role of A. indica (AI) and its chemopreventive effect on lead acetate (LA)-induced hepatocellular dysfunction with seventy adult male rats classified into group A- Control (distilled water), group B 0.1% LA only, group C and D- 0.1% LA + 100 mg/kg and 0.1% LA + 200 mg/kg AI respectively, group E- 0.2% LA, group F and G- 0.2% LA + 100 mg/kg and 0.2% LA + 200 mg/kg AI. Oxidative stress markers (MDA and H2O2), antioxidant parameters (GSH, SOD, CAT, GPx, GST), inflammatory markers (MPO and NO), alanine aminotransferase (ALT) and histopathological studies of the liver were evaluated. The results showed that LA administration caused a decrease in GSH, GPx, and GST while AI co-administration increased the activities of the antioxidants. Moreover, LA administration increased MPO, NO, MDA, and H2O2 levels whereas AI significantly reduced (P<0.05) these parameters. Histopathological examination revealed necrosis and mild infiltration by inflammatory cells in LA administered rats, whereas these lesions were absent in AI administered rats. In conclusion, A. indica demonstrates a protective role in lead acetate-induced hepatotoxicity, mainly via oxidative stress inhibition.
Haematological and biochemical changes in arsenic trioxide-induced toxicity in Wistar rats treated with methanol extract of Parquetina nigrescens leaf.
Daramola OO, Oyeyemi WAO, Akinola AOA … +1 more , Raji YR
Niger J Physiol Sci · 2022 Dec · PMID 38243571 · Publisher ↗
This study was designed to investigate the phytochemical composition and protective effects of methanol extract of Parquetina nigrescens leaf (MEPL) in male Wistar rats. Phytochemical screening, in vitro antioxidant assa...
This study was designed to investigate the phytochemical composition and protective effects of methanol extract of Parquetina nigrescens leaf (MEPL) in male Wistar rats. Phytochemical screening, in vitro antioxidant assay, gas chromatography-mass spectrometry (GC-MS) and LD50 were determined. Forty male Wistar rats were grouped into eight and orally treated for 54 days as follows: Group 1 (10% tween 80), Group 2 (3 mg/kg As2O3) Groups 3, 4 and 5 (250, 500 and 1000 mg/kg MEPL) and groups 6, 7 and 8, (250 mg/kg+As2O3, 500 mg/kg+As2O3 and 1000 mg/kg+As2O3). The animals were sacrificed on day 55 under anaesthesia. Blood was collected by cardiac puncture for heamatological studies. Liver concentrations of malondialdehyde (MDA), superoxide dismutase (SOD), aspartate aminotransferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) activities were determined spectrophotometrically. Liver histology was also assessed. Flavonoids, tannin, alkaloids, saponin, and anthraquinone were present in MEPL, also, MEPL scavenged 2,2 diphenyl-1-picrylhydrazyl hydrate (DPPH) and Azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS+). The IC50 of MEPL required to chelate metal was also low. The GC-MS revealed the presence of 24 essential oil. The LD50 was > 5000 mg/kg. Packed cell volume and red blood cell count were significantly reduced in 1000 mg/kg MEPL group, white blood cell count and SOD activity reduced (P<0.05) in 3 mg/kg As2O3 when compared with control but increased in groups co-treated with As2O3 and 250, 500 or 1000 mg/kg + As2O3. MDA concentration, AST, ALT and ALP activities increased significantly in 3 mg/kg As2O3 group but decreased (P<0.05) in groups co-treated with As2O3 and 250, 500 or 1000 mg/kg. The methanol extract of Parquetina nigrescens leaf in male Wistar rats has antioxidant, hepatoprotective and white blood cell protective effects.
Neuroprotective Effects of Virgin Coconut Oil Supplemented Diet against Sodium Benzoate-induced Acetylcholinesterase Dysfunction and Cognitive Impairment: Role of Nrf2/NfKb Signaling Pathway.
Asuku A
Niger J Physiol Sci · 2022 Dec · PMID 38243570 · Publisher ↗
The effect of virgin coconut oil (VCO) supplemented diet on sodium benzoate (SB) - induced neurotoxicity in male Wistar rats was investigated. Twenty (20) male Wistar rats weighing 160-180g were divided into four (4) gro...
The effect of virgin coconut oil (VCO) supplemented diet on sodium benzoate (SB) - induced neurotoxicity in male Wistar rats was investigated. Twenty (20) male Wistar rats weighing 160-180g were divided into four (4) groups: Control which received 1ml of normal saline, SB-treated; received 200 mg/kg b.w, SB + Low Dose VCO-treated (SB + 5% VCO mixed with 95g of rat chow), and SB + High Dose VCO-treated (SB+ 15% VCO mixed with 85g of rat chow). The brain was processed for NRF-2, NF-kB, and acetylcholine esterase (AchE) gene expression levels. Also, the blood sample was processed for assessment of superoxide dismutase (SOD), catalase (CAT), and IL1B levels. One-way ANOVA and Tukey post hoc tests were used to analyze data. SB-treated rats with no intervention showed anxiety-like behavior and impaired memory as depicted by a significant (p<0.0001) increase in anxiety index, increase in brain NF-KB, increase in serum IL1B and increase in AchE gene expression level, reduction in the recognition ratio, decreased spontaneous alternation performance, decreased CAT and SOD levels and decreased NRF-2 expression level when compared to other groups (especially control and SB + 5% VCO). VCO supplemented diet (both 5% and 15%) significantly (p<0.0001) increased the CAT and SOD levels, increased the NRF-2 gene expression level, and significantly (p <0.0001) decreased the IL1-B level. Moreover, 5% VCO significantly (p<0.0001) decreased the anxiety index, decreased AchE and NFkB gene expression levels, increased spontaneous alternation performance, and increased recognition ratio compared to 15% VCO. VCO shows a neuroprotective effect in attenuating cognitive impairment and anxiety-like behavior in SB-induced model by modulating oxidative stress and inflammatory pathways, and also enhancing cholinergic neurotransmission. Keywords: Virgin coconut oil; sodium benzoate; acetylcholinesterase; catalase; superoxide dismutase; oxidative stress.
Consumption of Aqueous Leaf Extract of Jatropha tanjorensis Improves Fertility Potential and Gestational Outcome in Virgin Female Wistar Rats.
Ukoh I, Sunday B, Antai A … +3 more , Arikpo S, Nsa H, David I
Niger J Physiol Sci · 2022 Dec · PMID 38243569
In spite of the overwhelming patronage of the plant Jatropha tanjorensis (J.T) popularly called "Hospital Too Far" by pregnant and women of reproductive age for its supposed reproductive benefits, the scientific evidence...
In spite of the overwhelming patronage of the plant Jatropha tanjorensis (J.T) popularly called "Hospital Too Far" by pregnant and women of reproductive age for its supposed reproductive benefits, the scientific evidence are hardly known. This study thus sought to examine the effect of consumption of aqueous leaf of J.T extract on female fertility potential and gestational outcome using forty (40) virgin female Wistar rats weighing 120-150g. The extract LD50 was >5000 mg/kg. The female rats were divided into unmated and mated groups (n=10 rats per subgroups). Group A (unmated group) consisted of control which received 0.2mL of normal saline (vehicle) and J.T-treated which received 500mg/kg (orally) once daily for 28 days. For Group B (mated group), the treated dams received 500 mg/kg (orally) once daily prior to mating (2 weeks), during mating (1 week) and throughout gestation, while the control received 0.5ml of physiological saline orally during the same period. All animals had free access to food and water. For the unmated group, after 28 days of treatment samples were collected for hormonal assay. J.T increased follicle stimulating hormone (FSH) and estrogen. For subgroup B, the extract was assessed for it reproductive and gestational performance, as well as pregnancy outcome. Nine (9) J.T-treated rats against seven (7) of control got pregnant. J.T increased mean weight gain, food and water intake. The Birth weight, body length and tail length of pups whose mothers were treated with the aqueous leaf extract of J.T increased significantly. Intake of J.T was effective in boosting female reproductive health, pregnancy health and outcome.
Comparative influence of kolaviron and coenzyme Q10 on complex I activity, glutamate clearance, 3,4-dihydroxyphenethylamine metabolism, and redox stress in rotenone-induced neurotoxicity.
Akinmoladun AC, Saliu I, Abilogun O … +5 more , Ajibola OH, Amoo ZA, Ojo OB, Farombi EO, Olaleye MT
Niger J Physiol Sci · 2022 Dec · PMID 38243568 · Publisher ↗
3,4-dihydroxyphenethylamine (dopamine) depletion, inhibition of complex I activity, oxidative stress, and glutamate excitotoxicity are cardinal biochemical features of neurotoxicity induced by systemic unilateral infusio...
3,4-dihydroxyphenethylamine (dopamine) depletion, inhibition of complex I activity, oxidative stress, and glutamate excitotoxicity are cardinal biochemical features of neurotoxicity induced by systemic unilateral infusion of rotenone. Kolaviron (KV), a biflavonoid from Garcinia kola seeds, has been proven to have pharmacological effects against neurotoxicity. Coenzyme Q10 plays an essential role in mitochondrial oxidative phosphorylation and as an antioxidant. This study examined the comparative influence of kolaviron and coenzyme Q10 on complex I activity, dopamine metabolism, glutamate clearance, and redox stress in rotenone-induced neurotoxicity in the cortex, hippocampus, and striatum of the brain of rats. Adult Male Wistar rats were pretreated with 200 mg/kg KV or 100 mg/kg coenzyme Q10 for 7 days followed by administration of a progressive six doses of 1.5 mg/kg rotenone within the next 48 h after which the animals were euthanized and the brain excised. On the cortical, hippocampal, and striatal regions of the brain, complex I activity, dopamine metabolism, oxidative stress markers, as well as glutamate metabolism were carried out and analyzed. In all brain regions examined, KV and coenzyme Q10 pretreatment modulated complex I activity, ameliorated redox imbalance, and enhanced dopamine metabolism via increasing the activity of tyrosine hydroxylase and decreasing monoamine oxidase activity. KV facilitated glutamate clearance through augmentation of glutamate dehydrogenase and glutamine synthetase activities. The activity of KV was comparable to that of the mitochondrial membrane antioxidant compound, coenzyme Q10, this indicates that KV is a promising therapeutic agent in the treatment of Parkinson's disease and its activity compares well with coenzyme Q10.
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