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Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal Of Applied Physiology[JOURNAL]
Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal Of Applied Physiology[JOURNAL]
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200 papers
Titanium Nanoparticles in Theranostics: A Multifunctional Platform for Targeted Therapy and Advanced Diagnostics.
Khatale SD, Dighe PR
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Oct · PMID 41130733 · Publisher ↗
AIM: To assess the theranostic potential of titanium nanoparticles (TiNPs), especially titanium dioxide nanoclusters, by assessing their distinct physicochemical characteristics and uses in biomedical domains as gene tra...
AIM: To assess the theranostic potential of titanium nanoparticles (TiNPs), especially titanium dioxide nanoclusters, by assessing their distinct physicochemical characteristics and uses in biomedical domains as gene transport, immunotherapy, antimicrobial therapy, cancer, and biosensing. METHODS: The multifunctional properties of TiNPs were reviewed, with particular attention paid to their wide surface area, photo-reactivity, biocompatibility, and simplicity of surface modification. They were evaluated for use in drug delivery, photothermal and photodynamic therapy, biosensing, immunotherapy, gene therapy, antibacterial and antiviral activity, imaging (MRI, CT, fluorescence, optical, and photoacoustic imaging), and immunotherapy. RESULT AND DISCUSSION: TiNPs have been demonstrated to improve imaging accuracy and sensitivity while facilitating real-time therapy monitoring. Through targeted administration, they decreased systemic toxicity and increased therapeutic efficacy in oncology. Their production of reactive oxygen species (ROS) promotes their antiviral, antibacterial, and biofilm-inhibitory properties. TiNPs also make it easier to distribute checkpoint inhibitors for immunotherapy and to deliver genes through targeted uptake and electrostatic interactions. They also have high-resolution molecular imaging capabilities and perform better in biosensing and diagnostics. CONCLUSION: By integrating therapeutic and diagnostic properties on one platform, titanium-based nanostructures demonstrate notable theranostic potential. The development of next-generation targeted medicines and diagnostics, better treatment results, and customized therapies are all made possible by their multifunctional nature.
Degradation Studies and Method Development and Validation of Lanadelumab Using UV Detector in RP-HPLC.
Marakatham S, Kumar MS, Kavya A … +2 more , Ramu B, Mare PB
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Oct · PMID 41077475 · Publisher ↗
The RP-HPLC methodology was used to establish a straightforward, accurate, and precise method for estimating Lanadelumab. The following chromatographic conditions were used: 5. Mobile phase: 0.1% OPA buffer: Acetonitrile...
The RP-HPLC methodology was used to establish a straightforward, accurate, and precise method for estimating Lanadelumab. The following chromatographic conditions were used: 5. Mobile phase: 0.1% OPA buffer: Acetonitrile in a ratio of 65:35; 5. Stationary phase: Agilent C18 250 x 4.6 mm; 5. Detection wavelength: 228.0 nm; column temperature: 30 °C; diluent: 50:50 acetonitrile: water; retention time: 2.280 min. As the most efficient approach, conditions were finalized. The standard was injected six times to study the system appropriateness characteristics, and the results fell well within the acceptable range. An analysis of linearity was conducted at 25% to 150% levels, and the R2 score was 0.999. Standard precision was determined to be 0.8, whereas repeatable precision was found to be 0.5. 0.08µg/ml is the LOD, while 0.24µg/ml is the LOQ. The assay of the marketed formulation was conducted using the described method, and 100.14% was found.
Beyond Hemoglobin: A Review of Hemocyanin and the Biology of Purple Blood.
Tiwari G, Tiwari R
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Sep · PMID 40925714 · Publisher ↗
Hemocyanin is dissolved freely in hemolymph, the invertebrate blood substitute, in contrast to haemoglobin, which is encased in red blood cells. When oxygenated, this pigment gives mollusc and arthropod blood its charact...
Hemocyanin is dissolved freely in hemolymph, the invertebrate blood substitute, in contrast to haemoglobin, which is encased in red blood cells. When oxygenated, this pigment gives mollusc and arthropod blood its characteristic blue or purple hue. This review article delves into the fascinating biology of hemocyanin, the copper-based oxygen-carrying protein responsible for "purple blood" in many invertebrates, contrasting its characteristics with the more familiar iron-based hemoglobin. The review used a variety of sources from 2020 to 2025, including preprint sites (bioRxiv, medRxiv), grey literature/press-release outlets including EurekAlert! and ScienceDaily, PubMed, Embase, Scopus, Web of Science, BIOSIS, and Google Scholar. While hemocyanin's unique properties allow for adaptation to diverse environments, its direct application as an artificial human blood substitute faces significant biological and immunological hurdles. The report then transitions to a comprehensive overview of recent advancements in artificial human blood transfusion, focusing on hemoglobin-based oxygen carriers (HBOCs), perfluorocarbon-based oxygen carriers (PFCs), and stem cell-derived red blood cells. This analysis critically examines their development, clinical trial outcomes, and the persistent challenges in achieving safe, effective, and widely available blood alternatives, highlighting the distinct roles and limitations of hemocyanin-derived products primarily in immunomodulation rather than oxygen transport.
Advancements in Pharmaceutical Nanocrystals:A Comprehensive Review.
Shah AM
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Sep · PMID 40887456 · Publisher ↗
Poorly soluble small compounds pose challenges in drug formulation due to low solubility, bioavailability, and therapeutic efficacy. These compounds often struggle to effectively target the disease due to their limited s...
Poorly soluble small compounds pose challenges in drug formulation due to low solubility, bioavailability, and therapeutic efficacy. These compounds often struggle to effectively target the disease due to their limited solubility. A large particle size further complicates reaching the desired site of action in the body. Reducing particle size through micronization or nanonization can enhance the efficacy of these active substances. Various methods, such as precipitation, milling, and high-pressure homogenization, are used to create nanocrystals, which can be delivered via multiple routes, with oral administration being preferred for safety and patient compliance. Nanonization is a key process in improving bioavailability, transforming micronized particles into nanoparticles under 1000 nm.
Pharmaceutical Cocrystals: A Review on Design, Preparation, Application and Challenges.
Shelke SJ, Bhangare DH, Patil MD
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Sep · PMID 40887434 · Publisher ↗
Pharmaceutical cocrystals have emerged as a transformative approach in drug development, enhancing the physicochemical properties of active pharmaceutical ingredients (APIs) such as solubility, bioavailability, stability...
Pharmaceutical cocrystals have emerged as a transformative approach in drug development, enhancing the physicochemical properties of active pharmaceutical ingredients (APIs) such as solubility, bioavailability, stability, and dissolution rate without altering their pharmacological characteristics. Defined as multicomponent crystalline solids composed of two or more neutral molecules in a stoichiometric ratio, cocrystals are formed through non-ionic interactions like hydrogen bonding and π-π stacking. This review explores the evolution, design, preparation, and applications of pharmaceutical cocrystals, highlighting their ability to improve drug performance, enable controlled release, and offer intellectual property opportunities. Various preparation methods, including solvent-based (e.g., solvent evaporation, cooling crystallization) and solid-based (e.g., grinding, liquid-assisted grinding) techniques, are discussed alongside design approaches like hydrogen bonding propensity and the supramolecular synthonic approach. The review also addresses challenges such as molecular compatibility, thermodynamic barriers, and regulatory considerations. With regulatory acceptance from agencies like the FDA and ongoing advancements in crystal engineering, pharmaceutical cocrystals hold significant promise for optimizing drug delivery and formulation, necessitating further research to fully realize their potential in commercial applications.
Acetylcholinesterase Inhibitor-Based Nose‑to‑Brain Delivery of Donepezil‑Loaded Lipid Nanoemulsion for Alzheimer's Therapy.
Mohanty C, Dubey MR, Panigrahi S … +3 more , Singh S, Rathi S, Nath J
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Aug · PMID 40820521 · Publisher ↗
Intranasal delivery offers a promising route for direct drug transport to the central nervous system, bypassing the blood-brain barrier and improving therapeutic outcomes in neurodegenerative diseases like Alzheimer's di...
Intranasal delivery offers a promising route for direct drug transport to the central nervous system, bypassing the blood-brain barrier and improving therapeutic outcomes in neurodegenerative diseases like Alzheimer's disease. Donepezil, a widely prescribed drug for Alzheimer's, suffers from poor oral bioavailability, delayed onset, and limited nootropic activity due to extensive systemic metabolism. To address these limitations, this study aimed to develop and optimize a Donepezil-loaded lipid-based nanoemulsion for enhanced nose-to-brain delivery. A Box-Behnken Design (BBD) was employed to optimize three formulation variables: drug-to-lipid ratio (1:2 to 1:6), surfactant concentration (1-2% w/v), and stirring speed (1500-2500 rpm), with their effects assessed on particle size, drug entrapment efficiency, and drug loading. Based on solubility and hydrophilic-lipophilic balance, Glyceryl Monostearate and Tween 80 were selected as excipients. Seventeen formulations were prepared and analyzed using Response Surface Methodology. The optimized formulation (Batch 18) exhibited a particle size of 160.12 nm, entrapment efficiency of 80.75%, and drug loading of 19.98%, with a desirability score of 0.977. Predicted and observed values were within ±5% variation, confirming model reliability with high Adjusted R² (>0.95), Predicted R² (>0.90), and a non-significant lack of fit (p > 0.05) by ANOVA. The optimized nanoemulsion showed enhanced brain-targeting efficiency and improved nootropic potential of Donepezil via the intranasal route, presenting a promising strategy for Alzheimer's therapy. However, the study was limited to in vitro assessments, and further in vivo pharmacokinetic, pharmacodynamic, and long-term safety evaluations are warranted to comprehensively establish its therapeutic potential.
CD3-Bispecific Monoclonal Antibodies: A Novel Therapeutic Approach for Complex and Multifactorial Diseases.
Singh S, Rathi S, Singh S … +2 more , Sharma B, Dwivedi V
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Aug · PMID 40754469 · Publisher ↗
Monoclonal antibodies (mAbs) have witnessed significant advancements in recent years, offering promising therapeutic options for the management of complex and multifactorial diseases. Despite their success, conventional...
Monoclonal antibodies (mAbs) have witnessed significant advancements in recent years, offering promising therapeutic options for the management of complex and multifactorial diseases. Despite their success, conventional mAbs exhibit limitations such as restricted targeting capacity and suboptimal immune activation, which has driven the development of bispecific monoclonal antibodies (BsAbs) capable of engaging multiple antigens simultaneously. Among these, CD3-bispecific mAbs have emerged as a potent class of immunotherapeutics, capable of activating T cells and inducing T cell-mediated cytotoxicity against target cells, particularly in cancer immunotherapy. This review highlights several representative formats of BsAbs, elucidates their underlying mechanisms of action, and discusses current design strategies for CD3-bispecific mAbs. Emphasis is placed on optimizing their therapeutic efficacy while minimizing adverse effects, supported by recent drug development examples and clinical applications.
Cancer Research in the 21st Century: Recent Advances and Future Perspectives.
Patole VP, Shinde NV, Chandak SM … +1 more , Satalkar AT
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jul · PMID 40628691 · Publisher ↗
INTRODUCTION: Cancer remains a major global health concern, accounting for nearly 10 million deaths annually. Despite its complexity and heterogeneity, significant advancements in cancer research over the past two decade...
INTRODUCTION: Cancer remains a major global health concern, accounting for nearly 10 million deaths annually. Despite its complexity and heterogeneity, significant advancements in cancer research over the past two decades have transformed the landscape of cancer diagnosis, treatment, and prevention. Notably, the integration of personalized medicine and technological innovations has led to more precise, effective, and individualized care strategies. MATERIALS: This review utilized peer-reviewed articles, clinical trial data, and recent meta-analyses published between 2015 and 2025. Major databases including PubMed, Scopus, and Web of Science were searched using keywords such as "cancer therapy," "personalized medicine," "cancer diagnostics," "immunotherapy," and "cancer prevention. METHODS: A systematic review approach was applied, focusing on studies that reported significant advancements in cancer treatment modalities (e.g., targeted therapy, immunotherapy), diagnostic technologies (e.g., liquid biopsy, AI-based imaging), and preventive strategies (e.g., vaccination, genetic screening). Articles were selected based on relevance, impact, and recency, with a preference for clinical studies and high-impact reviews. RESULTS: Emerging therapies such as immune checkpoint inhibitors, CAR-T cell therapy, and molecularly targeted agents have shown improved survival and response rates in several cancer types. Diagnostic innovations, including next-generation sequencing and non-invasive liquid biopsies, have enhanced early detection and monitoring of treatment response. Preventive measures, such as HPV and HBV vaccination and genetic risk profiling, have reduced the incidence of several preventable cancers. Personalized medicine approaches have enabled treatment decisions based on individual genetic and molecular profiles, leading to improved therapeutic outcomes and reduced adverse effects. DISCUSSION: The integration of genomics, artificial intelligence, and immunotherapy into oncology practice marks a shift toward precision medicine. While these advances have significantly improved patient care, challenges such as treatment resistance, access disparities, and the high cost of novel therapies remain. Continued interdisciplinary research, equitable healthcare policies, and investment in emerging technologies are essential to fully realize the benefits of modern cancer care.
Golden Flaxseed (Linum Usitatissimum): Unveiling Its Botanical Marvels, Therapeutic Potentials, and Nanoscale Applications.
Shinde NV, Sahane NS, Bhosale SK … +2 more , Patole VP, Satalkar AT
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jul · PMID 40628690 · Publisher ↗
Flax seeds, scientifically known as Linum usitatissimum, are nutrient-rich seeds with omega-3 fatty acids, lignans, and fiber. They offer diverse health benefits including cholesterol reduction, cardiovascular support, a...
Flax seeds, scientifically known as Linum usitatissimum, are nutrient-rich seeds with omega-3 fatty acids, lignans, and fiber. They offer diverse health benefits including cholesterol reduction, cardiovascular support, anti-inflammatory properties, and potential in combating chronic diseases like diabetes and cancer. Additionally, their anti-arrhythmic and anti-atherogenic properties benefit vascular health. Studying their botanical features, phytochemical composition, and pharmacological activities could lead to innovative medical treatments. Flax seeds, with their nutritional versatility, present a valuable addition to a balanced diet, potentially contributing to improved well-being and disease prevention. studies have demonstrated its effectiveness in the production of nanoparticles with enhanced pharmacological effects and potential applications in various biomedical fields. Therefore, the main purpose of this review is to explore the importantrole of L. usitatissimum as an important medicinal weed and to study its various pharmacological activities as well as the required chemical components.
A Pharmacological Study of Shirisharishta and Its Antihistaminic Effects in Guinea Pigs with Reference to Urticaria.
Chaurasia M, Nair PP, Saxena A
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jul · PMID 40619249 · Publisher ↗
The effect of Shirisharishta on histamine-induced bronchospasm in guinea pigs was evaluated in this study. Guinea pigs were placed in a histamine chamber, and an aerosol of 0.5% histamine was introduced using a compresso...
The effect of Shirisharishta on histamine-induced bronchospasm in guinea pigs was evaluated in this study. Guinea pigs were placed in a histamine chamber, and an aerosol of 0.5% histamine was introduced using a compressor and nebulizer. The antihistaminic activity of the compound formulation of Albizzia lebbeck bark, i.e., Shirisharishta, as well as the extracts of its individual Prakshepa Dravyas, was assessed against histamine aerosol-induced bronchospasm in guinea pigs. Additionally, a clinical trial of Shirisharishta was conducted on a group of patients suffering from urticaria, wherein its antiallergic effects were observed and compared to a standard treatment group. The study demonstrated promising antiallergic and bronchodilator activity of Shirisharishta, supporting its therapeutic potential in allergic and respiratory disorders.
A Comprehensive Overview of Traditional Chinese Medicine (TCM).
Dubey RK, Kumar B
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jul · PMID 40605540 · Publisher ↗
Traditional Chinese Medicine (TCM) represents a holistic approach to health and wellness that integrates ancient philosophies with diverse therapeutic modalities. Rooted in concepts of Yin-Yang balance, Qi (vital energy)...
Traditional Chinese Medicine (TCM) represents a holistic approach to health and wellness that integrates ancient philosophies with diverse therapeutic modalities. Rooted in concepts of Yin-Yang balance, Qi (vital energy), and the Five Elements theory, TCM aims to harmonize the body, mind, and spirit to achieve optimal health outcomes. This review explores the historical development, philosophical foundations, diagnostic methods, and common practices within TCM. It highlights the integration of acupuncture, herbal medicine, dietary therapy, and mind-body practices as key components of TCM's therapeutic arsenal. Scientific perspectives on TCM emphasize ongoing research efforts to validate its efficacy, mechanisms of action, and integration into modern healthcare systems. Criticisms surrounding TCM include challenges related to scientific validation, safety concerns regarding herbal products, and cultural differences in healthcare practices. Despite these challenges, TCM continues to evolve with advancements in research methodologies, digital health technologies, and integrative medicine approaches. The future of TCM holds promise in personalized and preventive medicine, global standardization, and ethical practices, positioning it as a valuable contributor to comprehensive healthcare models worldwide.
Perspectives on Fecal Microbiota Transplantation: Uses and Modes of Administration.
Tiwari R, Paswan A, Tiwari G … +2 more , Reddy VJS, Posa MK
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jul · PMID 40589142 · Publisher ↗
Fecal microbiota Transplantation (FMT), often referred to as stool transplantation, fecal transfusion, and fecal bacteria therapy, is considered one of the most medical innovations of the 20th century. Fecal microbiota T...
Fecal microbiota Transplantation (FMT), often referred to as stool transplantation, fecal transfusion, and fecal bacteria therapy, is considered one of the most medical innovations of the 20th century. Fecal microbiota Transplantation entails filtering and dilution of a healthy donor's feces before injecting it into the recipient's digestive system. In China, it was first administered orally in the fourth century for diarrhea and food poisoning under the name "Yellow Soup." It has recently been widely employed in a variety of clinical settings, including cases of Clostridium difficile infection that are recurring and resistant. By replacing the unhealthy intestinal microbiota with a healthy bacterial community, the FMT treatment aims to enhance the intestinal flora. It also looks at neurological conditions where alterations in gut microbiota are prevalent. We have discussed FMT in the context of its use in conditions affecting the nerve system, such as neurological and other conditions (multiple sclerosis, Parkinson's disease, Alzheimer's disease, stroke, epilepsy, Amyotrophic lateral sclerosis, Tourette syndrome, neuropathic pain, Huntington's diseases, etc.), as well as the role of gut microbiota in many neurological disorders.
A Brief Review on Transdermal Patches.
Singh S, Rajput DS, Gupta N … +3 more , Sharma B, Rathi S, Singh A
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jun · PMID 40545439 · Publisher ↗
Transdermal drug delivery system was presented to overcome the difficulties of drug delivery especially oral route. A transdermal patch is a medicated adhesive patch that is placed on the skin to deliver a specific dose...
Transdermal drug delivery system was presented to overcome the difficulties of drug delivery especially oral route. A transdermal patch is a medicated adhesive patch that is placed on the skin to deliver a specific dose of medication through the skin and into the bloodstream. It promotes healing to an injured area of the body. An advantage of a transdermal drug delivery route over other types of delivery system such as oral, topical, i.v., i.m., etc. is that the patch provides a controlled release of the medication into the patient, usually through either a porous membrane covering a reservoir of medication or through body heat melting thin layers of medication embedded in the adhesive. The main disadvantage to transdermal delivery systems stems from the fact that the skin is a very effective barrier, as a result, only medications whose molecules are small can easily penetrate the skin, so it can be delivered by this method. This review article describes the overall introduction of transdermal patches including type of transdermal patches, method of preparation of transdermal patches and factor affecting etc.
Unlocking the Potential of Sotagliflozin in Diabetes Mellitus targeting SGLT 1 & SGLT 2: A Comprehensive Review.
Kumbhare M, Chandak S, Shaikh A … +5 more , Velhal S, Dukare A, Gode H, Pagere N, Ide B
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jun · PMID 40545438 · Publisher ↗
Sotagliflozin, a novel dual inhibitor of sodium-glucose cotransporters 1 and 2 (SGLT1/2), represents a promising therapeutic advancement for managing diabetes mellitus. By inhibiting SGLT1 in the small intestine and SGLT...
Sotagliflozin, a novel dual inhibitor of sodium-glucose cotransporters 1 and 2 (SGLT1/2), represents a promising therapeutic advancement for managing diabetes mellitus. By inhibiting SGLT1 in the small intestine and SGLT2 in the kidneys, sotagliflozin uniquely improves glycemic control through reduced postprandial glucose absorption and enhanced urinary glucose excretion. This dual mechanism has shown significant benefits for both type 1 and type 2 diabetes, including reduced insulin requirements, better glycemic control, weight loss, and improved cardiovascular and renal outcomes. Clinical trials have highlighted its potential to mitigate the risks of diabetic complications such as heart failure and chronic kidney disease. However, its use is associated with some side effects, including gastrointestinal disturbances, urinary tract infections, and an elevated risk of diabetic ketoacidosis. This review explores the chemistry, pharmacology, and therapeutic implications of sotagliflozin, emphasizing its unique dual-target approach and potential to address unmet needs in diabetes management.
Epigenetic Regulation in Neuroplasticity: Key to Understanding and Treating Neurological Diseases.
Minal N, Nilesh W, Akanksha K
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jun · PMID 40533395 · Publisher ↗
Epigenetic mechanisms-DNA methylation, histone modifications, and non-coding RNAs-integrate genetic programs with environmental cues to shape neural development, plasticity, and pathology. During neurogenesis, methylatio...
Epigenetic mechanisms-DNA methylation, histone modifications, and non-coding RNAs-integrate genetic programs with environmental cues to shape neural development, plasticity, and pathology. During neurogenesis, methylation patterns and histone marks direct stem cell fate and synapse formation, while microRNAs fine-tune gene expression. In the adult brain, rapid, reversible histone acetylation and activity-dependent non-coding RNAs underlie learning, memory, and injury responses. Environmental stressors, toxins, and diet can trigger maladaptive epigenetic changes, linking exposures to cognitive deficits and psychiatric risk. Aberrant methylation and histone landscapes are implicated in autism, Alzheimer's, and Parkinson's-altering synaptic scaffolding, amyloid processing, and neuronal survival-while dysregulated microRNAs serve as both biomarkers and intervention targets. Advances in single-cell methylome sequencing, ChIP-seq, and multi-omics are clarifying cell-type specificity, and emerging therapies (HDAC inhibitors, methyl donors, RNA-based tools) offer promise, pending precise delivery and safety optimizations.
Evaluation, Characterization of Antioxidant Activity for Novel Benzothiazole Derivatives.
Chandak SM, Kumbhare MR
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jun · PMID 40533394 · Publisher ↗
The present study focuses on the design, synthesis, in-silico studies, characterization, and evaluation of the antidiabetic activity of nitrogen and sulphur-containing heterocyclic compounds, specifically benzothiazole d...
The present study focuses on the design, synthesis, in-silico studies, characterization, and evaluation of the antidiabetic activity of nitrogen and sulphur-containing heterocyclic compounds, specifically benzothiazole derivatives (2a-2e). The synthesized compounds were characterized using spectroscopic techniques such as IR, NMR, and mass spectrometry. Their biological activity was assessed through molecular docking studies, which revealed strong binding interactions with target proteins, with compound 2a exhibiting the highest docking score (-8.7). Swiss ADME analysis was performed to evaluate pharmacokinetic properties, indicating good drug-likeness and bioavailability. In vitro antidiabetic activity was assessed using the α-amylase inhibition method, where compounds 2a and 2e demonstrated significant inhibitory potential with IC₅₀ values of 22.95±2.50µg/mL and 27.80±3.00µg/mL, respectively. These findings suggest that benzothiazole derivatives, particularly compound 2a, hold promise as potential antioxidant agents, warranting further investigation for therapeutic applications.
Bioanalytical Method Development and Validation of Dapagliflozin in Human Plasma Using RP-HPLC Method.
Dighe PR, Kumbhare MR
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jun · PMID 40484655 · Publisher ↗
BACKGROUND: Dapagliflozin is used for controlling blood glucose levels in patients with type 2 diabetes. It is a sodium-glucose cotransporter 2 inhibitor, which enhances the elimination of blood glucose through the urine...
BACKGROUND: Dapagliflozin is used for controlling blood glucose levels in patients with type 2 diabetes. It is a sodium-glucose cotransporter 2 inhibitor, which enhances the elimination of blood glucose through the urine by inhibiting the protein involved in the transport mechanism of SGLT2. Dapagliflozin requires a selective and sensitive bioanalytical RP-HPLC method. AIM: Reverse phase - high performance liquid chromatography technique was used to develop and validate a bioanalytical method for the quantification of dapagliflozin (DAPA) in human plasma. METHODS: The internal standard (IS) used was azilsartan medoxomil. In isocratic mode, the mobile phase consisted of 50:50 v/v acetonitrile and 0.1% orthophosphoric acid in water at a flow rate of 1.0 mL/min. The chromatogram was recorded at 224 nm. For the chromatographic separation, a Kromasil C18 column (250 mm × 4.6 mm; 5μ) was used. The drug was extracted from plasma samples by the protein precipitation method. RESULT AND DISCUSSION: The chromatographic run time was 15 min. Dapagliflozin and IS eluted at 4.6 and 5.7 min, respectively. The method was selective and sensitive, with a limit of quantification of 1.50 µg/mL. The developed method was found to be linear in the range of 1.50-60 µg/mL (r2 = 0.9994). The accuracy and precision obtained from six sets of quality control (QC) samples ranged from 96.23% to 108.67% and 1.35% to 3.19%, respectively. The extraction recovery of dapagliflozin in three QC samples ranged from 87.39% to 90.78%. The bench-top stability, stock solution stability, stability of processed extracted samples at room temperature, and freeze-thaw stability evaluations showed no evidence of degradation of dapagliflozin. CONCLUSION: The stability, selectivity, sensitivity, and reproducibility of the developed method make it suitable for the determination of dapagliflozin in human plasma.
Natural Flavonoids as Anticancer Agents: Targeting the HIF-1α Signaling Pathway.
Shinde N, Satalkar A, Bhosale S … +2 more , Patole V, Agale S
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jun · PMID 40484654 · Publisher ↗
BACKGROUND: The clinical effectiveness of wide range of currently available anticancer drugs is being reduced .HIF-1 alpha is essential for the reprogramming of cancer cells' metabolism,so cancer treatments include inhib...
BACKGROUND: The clinical effectiveness of wide range of currently available anticancer drugs is being reduced .HIF-1 alpha is essential for the reprogramming of cancer cells' metabolism,so cancer treatments include inhibiting the HIF-1α signaling pathway and The evidence underscores the potential of natural flavonoids as HIF-1α inhibitors in cancer therapy. OBJECTIVE: To provide more comprehensive overview of inhibition of flavonoids on HIF-1 which may be useful for developing new compounds with enhanced anticancer properties and also to provide reasearchers with a thought to design potent and low toxic anticancer candidates. MATERIAL AND METHODS: A comprehensive review of recent literature was conducted to identify studies investigating the effects of natural flavonoids and their derivatives on HIF-1α activity. Emphasis was placed on mechanisms of action, efficacy, and toxicity profiles, as well as their impact on tumor hypoxia and associated pathways such as vascularization and glycolysis. RESULT: Recent findings demonstrate that various natural flavonoids effectively downregulate HIF-1α through distinct mechanisms. These compounds exhibit significant anti-cancer properties while maintaining low toxicity, making them viable candidates for further development. Several studies have also highlighted the ability of flavonoids to influence tumor vascularization and glycolytic pathways, thereby enhancing their therapeutic potential. CONCLUSION: In this review in order to increase bioavailability , solubility and to better understand the impact of anticancer flavonoids on HIF-1 alpha, amino acids or amino groups were added to the flavonoid structure. understanding the effect of anticancer flavonoids on HIF-1 α may be relevant in the development of novel compounds with increased anticancer activity.
Targeting FLT3 Mutations in Acute Myeloid Leukemia: The Role of Quizartinib in Precision Medicine.
Kumbhare M, Chandak S, Ide B … +5 more , Dukare A, Velhal S, Gode H, Pagere N, Shaikh A
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 Jun · PMID 40456703 · Publisher ↗
Acute Myeloid leukemia (AML) is a genetically heterogeneous hematologic malignancy that disproportionately affects older individuals. Among the various genetic alterations, FLT3 internal tendem duplication (FLT3-ITD) mut...
Acute Myeloid leukemia (AML) is a genetically heterogeneous hematologic malignancy that disproportionately affects older individuals. Among the various genetic alterations, FLT3 internal tendem duplication (FLT3-ITD) mutations are present in approximately 20-30% of patients and are linked to rapid disease progression and frequent relapses. This review evaluates the role of quizartinib, a second-generation, highly selective FLT3 inhibitor, as a targeted therapeutic option for relapsed or refractory AML. Preclinical studies have demonstrated that quizartinib offers potent inhibition of FLT3 signaling, favorable pharmacokinetic properties, and high bioavailability. Early-phase clinical trials reported promising remission rates in patients harboring FLT3-ITD mutations, while phase III studies further substantiated its efficacy by showing improved overall survival when used alone or alongside standard chemotherapy. Despite these advances, quizartinib's clinical use is limited by challenges such as acquired resistance, off-target effects-including QT interval prolongation-and complex drug-drug interactions. Ongoing research is focused on elucidating resistance mechanisms and developing effective combination regimens to optimize its therapeutic potential. Overall, quizartinib represents a significant breakthrough in precision medicine for AML, offering a promising avenue to improve patient outcomes in this challenging disease.
Mechanistic Insights and Emerging Applications of Nanogel-Based Topical Drug Delivery Systems.
Kumbhare M, Shinde P, Mohite D … +3 more , Rokade V, Chandak S, Dukare A
Zhongguo Ying Yong Sheng Li Xue Za Zhi · 2025 May · PMID 40389329 · Publisher ↗
PURPOSE: Nanogels (NGs) represent a new generation of nanoscale hydrogel particles with immense biomedical applications, especially drug delivery System (DDS). Nanogels (NGs) are soft and water-retentive materials that d...
PURPOSE: Nanogels (NGs) represent a new generation of nanoscale hydrogel particles with immense biomedical applications, especially drug delivery System (DDS). Nanogels (NGs) are soft and water-retentive materials that deliver drugs in controlled and sustained release while maintaining high biocompatibility and biodegradability. METHOD: This review paper primarily focuses on the applications, mechanism, therapies. The capability to react with environmental signals, such as pH, temperature, or enzymatic activity, makes nanogels a promising platform for targeted therapy. Polysaccharide-based nanogels increase biocompatibility and reduce toxicity, allowing for extended therapeutic applications. RESULT: This reviews the structural properties, drug release mechanisms, and newer developments in nanogel formulations. It also presents the future outlook for nanogels in precision medicine, highlighting their potential in transcending pharmacological obstacles towards enhancing drug delivery and therapeutic efficacies in various branches of medicine. CONCLUSION: By overcoming existing drawbacks, nanogels have the potential to revolutionize drug delivery and therapeutic interventions in diverse clinical settings.
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