Colloids And Surfaces. B, Biointerfaces[JOURNAL]
Sun
200 papers
Suppressing angiogenic phenotypes in retinal cell models through light-triggered anti-VEGF release from upconversion nanoparticle-loaded chitosan microgels.
Canbulat Z, Yalcin E, Eroglu Z … +4 more , Khadra R, Hasanreisoglu M, Metin O, Kizilel S
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42372461 · Publisher ↗
Anti-VEGF injections are effective for neovascular age-related macular degeneration (nAMD) but require frequent intravitreal dosing and provide limited spatiotemporal control. We engineered injectable chitosan-methacryla...
Anti-VEGF injections are effective for neovascular age-related macular degeneration (nAMD) but require frequent intravitreal dosing and provide limited spatiotemporal control. We engineered injectable chitosan-methacrylate (ChiMA) microgels that co-localize upconversion nanoparticles (UCNPs) with a photocaged anti-VEGF peptide to enable on-demand, near-infrared (980 nm)-triggered release. Colloidal UCNPs (β-NaYF₄: Yb,Tm core/shell) were synthesized via a thermal decomposition method and subsequently embedded into visible-light-crosslinked ChiMA microgels together with a nitrobenzyl-linked anti-VEGF peptide. We quantified microgel morphology, swelling and injectability, peptide loading/release under near infra-red (NIR) irradiation (20 mW/cm) and durations (30 min), and cytocompatibility in MIO-M1, RPE-1, and HUVEC cells. Anti-angiogenic activity was assessed by VEGF-driven scratch migration, tube formation, and signaling (HIF-1α, p-Akt, p-p38, occludin, ZO-1). Microgels with 10 wt% UCNPs showed the most efficient NIR-triggered release around 85%. Cytocompatibility assays indicated approximately 90% viability for UCNPs-loaded microgels, consistent with minimal cytotoxicity. In vitro, NIR irradiation of anti-VEGF-peptide-functionalized ChiMA microgels reduced endothelial migration and tube formation by 20% and 15%, respectively, relative to VEGF-stimulated controls. VEGF-responsive signaling was concomitantly decreased by 50%, and Akt phosphorylation was likewise halved compared to VEGF alone. These findings indicate that UCNPs-loaded ChiMA microgels enable non-invasive, repeatable, on-demand peptide delivery and attenuate pro-angiogenic pathways, supporting subsequent in vivo evaluation.
Phospholipid membrane interfaces and metal cations, Ca and Cu, modulate insulin amyloid fibrillation.
Tsurumoto S, Goto S, Kasai T … +5 more , Sakaguchi T, Shiratori T, Horizumi Y, Wada T, Tsuchida T
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42372460 · Publisher ↗
Metal ions are widely implicated in amyloid fibril (AF) formation, yet their effects have largely been characterized in solution, while the influence of lipid membrane interfaces has received less attention. To address t...
Metal ions are widely implicated in amyloid fibril (AF) formation, yet their effects have largely been characterized in solution, while the influence of lipid membrane interfaces has received less attention. To address this gap, we herein addressed the question of whether the effects of two divalent cations with contrasting physicochemical properties (Cu and Ca) on AF formation in a model amyloidogenic protein (recombinant human insulin, INS) are the intrinsic properties of these ions in solution or depend on the presence of a lipid membrane interface. AF formation kinetics were monitored using thioflavin T fluorescence, ion accumulation at colloidal surfaces was assessed by zeta potential measurements, and the secondary structural composition of aggregates was characterized by attenuated total reflection Fourier transform infrared spectroscopy and analyzed using singular value decomposition (SVD). In the absence of egg yolk phospholipids small unilamellar vesicles (SUVs), Ca accelerated nucleation without altering AF structure, whereas Cu inhibited AF nucleation and elongation in a concentration-dependent manner. The introduction of SUVs transformed the direction and magnitude of these effects: Ca shifted from being a nucleation promoter to acting as an AF elongation inhibitor, whereas Cu inhibition was amplified to near-complete suppression at 100 μM. Zeta potential analysis revealed an apparent depletion of free Cu consistent with binding via direct INS coordination below 100 μM-a pattern absent for Ca-and SVD analysis showed that Cu progressively disrupted cross-β structure formation, whereas Ca caused no structural change under any condition. These results suggest that the membrane interface does not merely modulate the magnitude of metal ion effects on AF formation but determines their direction. The outcome is governed by the physicochemical identity of each ion: Ca acts through nonspecific electrostatic screening and is redirected at the membrane to suppress elongation via interfacial confinement; Cu acts through direct protein coordination and is amplified at the membrane by geometric concentration at the two-dimensional interface. The role of metal ions in AF formation cannot be assessed using solution-phase measurements alone, as the membrane interface is an integral determinant of the aggregation outcome.
Corrigendum to "Multifunctional piezoelectric surfaces enhanced with layer-by-layer coating for improved osseointegration and antibacterial performance" [Colloids Surf. B: Biointerfaces 243 (2024) 114123].
Carvalho EO, Fernandes MM, Ivanova K … +4 more , Rodriguez-Lejarraga P, Tzanov T, Ribeiro C, Lanceros-Mendez S
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42364955 · Publisher ↗
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Cooperative effects of natural acetylenic acetogenins fromPorcelia macrocarpaon lipid packing and mechanical stability of Langmuir monolayers as protozoan membrane models.
Rosa ME, Brito IA, Lago JHG … +1 more , Caseli L
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42364323 · Publisher ↗
This study investigates the synergistic interfacial behavior of two natural bioactive acetylenic acetogenins, (2S,3R,4R)-3 hydroxy-4-methyl-2-(n-eicos-11'-yn-19'-enyl)butanolide (1) and (2S,3R,4R)-3-hydroxy-4 methyl-2-(n...
This study investigates the synergistic interfacial behavior of two natural bioactive acetylenic acetogenins, (2S,3R,4R)-3 hydroxy-4-methyl-2-(n-eicos-11'-yn-19'-enyl)butanolide (1) and (2S,3R,4R)-3-hydroxy-4 methyl-2-(n-eicos-11'-ynyl)butanolide (2), obtained from Brazilian plant Porcelia macrocarpa (Annonaceae) and their interactions with membrane models using Langmuir films. Previous biological studies demonstrated that mixtures of 1 and 2 exhibit enhanced antiparasitic activity compared to the isolated compounds, although the physicochemical basis for this synergism remained unclear. In the present study, equimolar mixtures of 1 and 2 were investigated through surface pressure-area (π-A) isotherms, surface compressional modulus, Brewster Angle Microscopy (BAM), and rheological analyses, both as pure films and incorporated into dipalmitoylphosphatidylethanolamine (DPPE) monolayers, used as protozoan membrane models. The mixture of 1 and 2 monolayer exhibited distinct interfacial organization compared to the pure compounds, including suppression of phase-transition regions characteristic of 2, delayed onset of pre-collapse instabilities, and enhanced condensation before collapse. BAM images revealed cooperative effects between the compounds, leading to more homogeneous monolayers prior to instability and more interconnected collapse structures after compression. When incorporated into DPPE monolayers, the mixture of 1 and 2 induced expansion, phase separation, and mechanical instabilities at biologically relevant surface, indicating substantial perturbation of membrane packing. The combined results demonstrate that the interplay between the different unsaturation patterns of the studied acetogenins modulates molecular organization, phase behavior, and membrane-disruptive properties at interfaces. These findings provide physicochemical insights into the synergistic bioactivity previously observed for acetogenin mixtures and highlight the importance of collective intermolecular interactions in the membrane activity of natural products.
Multifunctional SiO&PB particles composite coating on a titanium surface enhances the osseointegration of dental implants in diabetes.
Jia S, Zeng D, Zhang M … +9 more , Liao L, Zhou H, Huang L, Jiang Z, Zhang H, Dong Y, Cai C, Shen X, Ding X
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42361593 · Publisher ↗
Titanium (Ti) implants often fail to perform optimally because of biological inertness and limited antimicrobial activity, especially in patients with systemic conditions like diabetes or osteoporosis. In this study, a p...
Titanium (Ti) implants often fail to perform optimally because of biological inertness and limited antimicrobial activity, especially in patients with systemic conditions like diabetes or osteoporosis. In this study, a porous oxide layer doped with silica (SiO) particles was formed on Ti via micro-arc oxidation (MAO), and Prussian blue (PB) particles were immobilized onto the surface using polydopamine (PDA) adsorption to create a SiO&PB composite coating. The coating features micro/nanoscale roughness, strong hydrophilicity, good wear and corrosion resistance, and a near-infrared (NIR) photothermal effect; in vitro it demonstrated antibacterial effects, reactive oxygen species (ROS) scavenging, and promoted osteogenic differentiation. In a diabetic rat extraction-socket model, the coating enhanced early osseointegration, indicating a promising strategy to reduce implant failure in patients with systemic diseases and potential for clinical translation.
Fluorescence-trackable chitosan/UCNPs coatings regulate magnesium alloy corrosion and osteogenesis.
Sun M, Sun D, Yin X … +6 more , Wang Y, Shi Z, Chen J, Xiong G, Zhang Y, Wang C
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42361592 · Publisher ↗
Rapid corrosion and the absence of non-invasive readouts limit the clinical translation of biodegradable magnesium implants. Here, AZ31 magnesium alloy was alkali-treated and coated with chitosan (AZ31-CS) or a chitosan/...
Rapid corrosion and the absence of non-invasive readouts limit the clinical translation of biodegradable magnesium implants. Here, AZ31 magnesium alloy was alkali-treated and coated with chitosan (AZ31-CS) or a chitosan/upconversion nanoparticle composite (AZ31-CN; NaYF₄:Yb⁺,Er⁺ ) to couple corrosion control with fluorescence-guided degradation tracking. In simulated body fluid, chitosan-based coatings reduced corrosion rate, pH increase, and hydrogen evolution compared with AZ31-OH. For AZ31-CN, near-infrared excitation generated visible emission, and fluorescence intensity decreased during immersion, serving as a surrogate indicator of coating degradation. MC3T3-E1 assays demonstrated good cytocompatibility, enhanced proliferation, and increased osteogenic differentiation, evidenced by elevated ALP activity, mineralization, and upregulated ALP, OPN, and RunX2 expression. In a rat calvarial defect model, coated samples showed higher bone volume fraction and trabecular number than AZ31-OH. The chitosan/UCNPs coating provides a multifunctional biointerface integrating degradation regulation with optical monitoring for traceable magnesium-based bone implants.
Quatsome nanovesicles as antibacterial platform: Mechanistic insights into their activity against planktonic and biofilm Staphylococcus aureus.
Köber M, Gallardo-Moreno AM, Ferrer-Tasies L … +17 more , Fernández-Calderón MC, Pujol-Solé N, Tomsen-Melero J, Guasch E, Tamurejo-Alonso P, Mitjans M, Vinardell MP, Domingo-Tafalla B, Giannotti MI, Rancan F, Schaudinn C, Veciana J, Ratera I, Roldán M, González-Mira E, González-Martín ML, Ventosa N
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42361591 · Publisher ↗
The growing threat of antibiotic-resistant pathogens has intensified the demand for alternative antibacterial materials. Quatsomes-nanovesicles composed of cholesterol and quaternary ammonium surfactants (QAS)-emerge as...
The growing threat of antibiotic-resistant pathogens has intensified the demand for alternative antibacterial materials. Quatsomes-nanovesicles composed of cholesterol and quaternary ammonium surfactants (QAS)-emerge as promising candidates due to their intrinsic antimicrobial properties and tunable physicochemical characteristics. Here, we investigate the antibacterial activity of quatsomes incorporating QAS with either tetradecyl (C14) or hexadecyl (C16) alkyl chains against Staphylococcus aureus, a leading cause of hospital-acquired infections. Both quatsome types exhibited potent bactericidal activity in planktonic cultures, with C16-containing formulations showing a 2.5-fold lower minimum bactericidal concentration than C14 counterparts. Confocal microscopy suggested a partial penetration of cationic quatsomes into the bacterial peptidoglycan layer, accompanied by significant increases in ζ-potential, suggesting strong electrostatic interactions without visible membrane disruption, as confirmed by scanning electron microscopy. Both formulations also demonstrated high efficacy against mature S. aureus biofilms, with no significant differences between alkyl chain lengths, indicating a mechanism primarily targeting the extracellular biofilm matrix. In addition, they showed a good antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). A preliminary safety assessment using reconstructed human epidermis (Episkin™) confirmed the non-irritant nature of both formulations. These findings highlight the potential of QAS-based quatsomes as effective and biocompatible nanocarriers for topical antibacterial applications, offering a promising platform for combating antibiotic-resistant infections in both planktonic and biofilm states.
Extracellular matrix-inspired biomimetic construction by IKVAV-functionalized ovalbumin hydrogel for regulating Schwann cells and DRG growth in nerve regeneration.
Wang L, Jia M, Jin X … +8 more , Shang Y, Zhao Y, Shen H, Shi L, Sun J, Guo J, Li G, Wu X
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42361590 · Publisher ↗
Peripheral nerve injury repair remains a prominent clinical challenge owing to the limited self-regenerative capacity of neural tissues and the lack of ideal biomimetic microenvironmental scaffolds. Hydrogels can mimic t...
Peripheral nerve injury repair remains a prominent clinical challenge owing to the limited self-regenerative capacity of neural tissues and the lack of ideal biomimetic microenvironmental scaffolds. Hydrogels can mimic the extracellular matrix but commonly lack specific bioactive signals to efficiently drive neural regeneration. To address this issue, we developed an IKVAV (Isoleucine-Lysine-Valine-Alanine-Valine) peptide-functionalized ovalbumin (OVA) hydrogel via chemical crosslinking and dopamine-mediated surface modification. Physicochemical characterizations demonstrated that the hydrogel possessed favorable gelation efficiency, tunable viscoelasticity, suitable mechanical strength, good hydrophilicity, and controllable swelling and degradation behaviors. Fourier transform infrared spectroscopy verified the successful immobilization of IKVAV peptides on the OVA hydrogel. In vitro cellular evaluations revealed that compared with pure OVA hydrogel, the IKVAV-functionalized hydrogel significantly improved the adhesion, proliferation, migration, and morphological elongation of Schwann cells (RSC96), and markedly promoted axonal growth and directional extension of dorsal root ganglion (DRG) neurons. Mechanistic studies using quantitative PCR and proteomic analysis confirmed that the functional scaffold significantly upregulated the expression of genes and proteins related to cell survival, axonal extension, myelination, and angiogenesis, possibly through activating the IKVAV-integrin-YAP (Yes-associated protein) signaling axis. These results indicate that the IKVAV-functionalized OVA hydrogel constructs a biomimetic neural regenerative microenvironment by integrating physical support and biological cues, thereby synergistically enhancing Schwann cell activity and axonal regeneration. This study provides a novel multifunctional hydrogel strategy for peripheral nerve tissue engineering and injury repair.
Smart responsive microneedles for drug delivery and therapeutic applications.
Liu Y, Wang J, Gao Y … +2 more , Sun C, Chen W
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42341645 · Publisher ↗
Drug delivery systems are essential to modern medicine, aiming to transport drugs precisely to target sites for enhancing drug efficacy and reducing corresponding side effects. As an innovative transdermal technology, mi...
Drug delivery systems are essential to modern medicine, aiming to transport drugs precisely to target sites for enhancing drug efficacy and reducing corresponding side effects. As an innovative transdermal technology, microneedle (MN)-based drug delivery offers minimal invasiveness, painlessness, and facile control. Smart responsive MNs represent a recent advancement, featuring bio-inspired structures, multilayer designs, flexible substrates, and wearable integration, all of which can improve patient compliance. These MNs can be prepared from various materials, and their structures and functions can be tailored by adjusting their compositions, response mechanisms, and fabrication methods to match specific applications. Upon exposure to specific stimuli, smart responsive MNs enable precise, controllable drug release in complex biological environments. Multi-responsive systems further enhance targeting and release control by integrating multiple stimulus-response pathways. Currently, smart responsive MNs are being explored for drug delivery, vaccination, cosmetics, diagnostics, tissue engineering, cancer therapy, and wound healing. However, reviews in this area remain limited. Accordingly, this review outlines the design strategies, emerging technologies, innovative functions, and clinical uses of smart responsive MNs, while discussing key challenges and future directions. It provides insights to support research on novel MN-based drug delivery and therapy.
Poly(glycerol)-modified liposome for co-delivery of pheophorbide a/ topotecan and combination cancer therapy in vivo.
Kim SH, Gi Lim S, Hui Park C … +5 more , Young Cheon S, Chun S, Nam H, Cho B, Koo H
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42341644 · Publisher ↗
The clinical success of nanoparticles (NPs) with surface coating using polyethylene glycol (PEG) has been accompanied by growing concerns regarding PEG-induced hypersensitivity and immunogenicity. These limitations have...
The clinical success of nanoparticles (NPs) with surface coating using polyethylene glycol (PEG) has been accompanied by growing concerns regarding PEG-induced hypersensitivity and immunogenicity. These limitations have spurred the search for PEG alternatives, particularly in cancer therapy, where repeated administration is common. Polyglycerol (PG), a hydrophilic and biocompatible polymer, has emerged as a promising candidate for surface coating of NPs to its antifouling properties and reduced recognition by macrophages. In this study, we developed a PG-coated liposomal nanomedicine loaded with topotecan (Tpt), a hydrophilic anticancer drug, and pheophorbide a (Pba), a hydrophobic photosensitizer. This dual-loading strategy enables two complementary therapeutic mechanisms: DNA repair inhibition by Tpt and reactive oxygen species (ROS)-mediated photodynamic therapy (PDT) by Pba under 671 nm laser irradiation. The dual drug-loaded PG-liposome (Pba/Tpt@PG) exhibited potent cytotoxicity against SCC7 tumor cells, demonstrated reduced macrophage uptake in vitro, and in vivo imaging revealed its favorable tumor accumulation. SCC7 tumor-bearing mice received intravenous injections of Pba/Tpt@PG, followed by localized laser irradiation. This treatment successfully inhibited tumor growth, reducing tumor volume to 0.58% of that in untreated controls, and outperformed single-agent treatments. No significant body weight loss or histological abnormalities were observed, indicating high biocompatibility of the materials. This study underscores the potential of dual-drug-loaded liposomes and PG-based surface modification for safe and effective combination cancer therapy.
A photoactivated nanoreactor cascade amplifies immunogenic endoplasmic reticulum stress for hepatocellular carcinoma immunotherapy.
Zeng X, Zhao Y, Xing S … +2 more , Hu B, Ding C
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42335790 · Publisher ↗
Immunotherapy has emerged as a promising strategy for hepatocellular carcinoma (HCC), its effectiveness is significantly limited by insufficient immunogenic cell death (ICD). Here, we developed a photoactivated nanoreact...
Immunotherapy has emerged as a promising strategy for hepatocellular carcinoma (HCC), its effectiveness is significantly limited by insufficient immunogenic cell death (ICD). Here, we developed a photoactivated nanoreactor (ADDT) by integrating a trans-vaccenic acid (TVA)-coated degradable dendritic mesoporous silica nanoparticle (DMSN) core co-loaded with Au NPs and doxorubicin (DOX) to induce cascade-mediated endoplasmic reticulum (ER) stress in HCC therapy. Near-infrared irradiation melted the TVA through the photothermal conversion of aggregated Au NPs, triggering GSH-responsive DMSN degradation. The released Au NPs deplete glucose, and together with mild photothermal therapy and DOX chemotherapy, significantly increase ROS production and amplify ER stress. This cascade culminates in a potent ICD effect. The released tumor-specific antigens facilitate dendritic cell maturation, while TVA further enhances tumor infiltration by CD8 T cells. These synergistic actions effectively suppress both primary tumors and pulmonary metastasis. This study provides a strategy to overcome the limitations of tumor immunogenicity and activate a potent antitumor immune response.
Genipin based green chemistry immobilization cascade towards enzyme based antibacterial biomaterial surfaces.
Alonso FJF, Huerta JRM, Aguilera-Correa JJ … +6 more , Cruz A, Cortés-Llanos B, de Sousa CT, Zuazo JR, Esteban J, Silván MM
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42335789 · Publisher ↗
Biomedical implants are highly susceptible to rapid bacterial colonization and biofilm formation, a major cause of implant failure and postsurgical complications. Although current antibacterial approaches commonly rely o...
Biomedical implants are highly susceptible to rapid bacterial colonization and biofilm formation, a major cause of implant failure and postsurgical complications. Although current antibacterial approaches commonly rely on metal-ion release or reactive oxygen species, these strategies may induce cytotoxicity or oxidative stress, underscoring the need for safer, biobased alternatives. Here we present a fully aqueous and green chemistry immobilization cascade to endow biomaterial surfaces with stable enzymatic antibacterial activity. Titanate assisted organosilanization provides an aminated surface onto which genipin, a low toxicity natural crosslinker, is covalently linked, enabling the immobilization of two antibacterial enzymes: lysozyme and α-amylase. Functionality assays reveal that genipin crosslinked lysozyme significantly reduces Clostridium perfringens viability, while α-amylase retains its catalytic activity, yielding sustained D-glucose production from maltose. L929 fibroblast cells exposed to 100% extracts obtained from the surfaces at different stages of enzymatic immobilization remained viable (≥80%) even for longer incubation periods (72 h). Altogether, this study establishes a mild, solvent free and biobased immobilization route capable of producing stable enzyme-functionalized surfaces, offering a versatile platform for future development of antibacterial biomaterial coatings.
Solar-simulated light-driven ROS-responsive CuₓO-TiO microneedle patch for intelligent antibacterial and regenerative therapy of infected burn wounds.
Tang Q, Luo WL, Guo AN … +3 more , Chen Y, Liu ZH, Shao JW
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42335788 · Publisher ↗
Burn wounds infected with Staphylococcus aureus (S. aureus) are often characterized by excessive early inflammation, which leads to persistent oxidative stress, pain, and delayed healing. To overcome the limitations of c...
Burn wounds infected with Staphylococcus aureus (S. aureus) are often characterized by excessive early inflammation, which leads to persistent oxidative stress, pain, and delayed healing. To overcome the limitations of conventional photosensitizers, which may induce reactive oxygen species (ROS) overload and subsequent tissue damage, we developed a ROS-responsive microneedle patch (TC@MN). This system consists of chlorogenic acid-based microneedles encapsulating a CuₓO-TiO (TC) nanozyme heterostructure designed for synergistic antibacterial and regenerative therapy. TC@MN exhibits intrinsic peroxidase-like (POD) and catalase-like activities (CAT). Under light irradiation, these catalytic processes participate in ROS regulation and promote Cu²⁺/Cu⁺ redox cycling, leading to the generation of bactericidal •OH and •O₂⁻ radicals while simultaneously producing O₂ to alleviate local hypoxia. In animal experiments, TC@MN scavenges excess ROS and promotes macrophage polarization toward the M2 phenotype thereby facilitating inflammation resolution and tissue repair under light-free conditions. Collectively, this light/dark dual-mode microneedle platform integrates photocatalytic and enzyme-mimetic functions, offering a biocompatible and intelligent strategy for the treatment of infected burn wounds.
Construction of chitosan-coated TiO/InS heterojunction on titanium surfaces for NIR triggered photothermal/photodynamic synergistic enhanced antibacterial.
Dong P, Liu Y, Deng F … +9 more , Zhao A, Ren M, Song L, Zhou Y, Dong Y, Xiang Y, D'Amora U, Xu L, Rao X
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42330607 · Publisher ↗
Implant-associated bacterial infections remain a major challenge in orthopedic and dental applications, often compromising implant integration and longevity. For this reason, the synergistic antibacterial strategy of pho...
Implant-associated bacterial infections remain a major challenge in orthopedic and dental applications, often compromising implant integration and longevity. For this reason, the synergistic antibacterial strategy of photothermal and photocatalysis has become one of the important methods in the field of antibacterial. To synthesize high-efficiency near-infrared (NIR) light-responsive catalysts, a titanium oxide/indium sulfide (TiO/InS) heterojunction antibacterial coating was prepared on the titanium (Ti)-based surface using anodic oxidation and wet chemical deposition methods. Compared with Ti alloy, the TiO/InS heterojunction showed a suitable energy band structure, improved light absorption, and enhanced carrier separation capability, thus enhancing photothermal catalytic activity and promoting the production of bacterial reactive oxygen species (ROS) under NIR light. Indeed, the TiO/InS heterojunction narrowed the bandgap (∼2.5 eV) and efficiently generated ROS (•O₂⁻ and •OH) under NIR irradiation. Furthermore, the successful deposition of chitosan (CS) endowed the TiO/InS heterojunction with osteogenic activity, promoting the proliferation and differentiation of osteoblasts. In vitro, results demonstrated effective antibacterial performance against Escherichia coli and Staphylococcus aureus, inhibited biofilm formation, and supported collagen secretion and mineralization. In vivo, NIR irradiation raised the implant site temperature to below 50 °C, enabling safe and efficient sterilization, and showing no organ toxicity. These results highlight CS-coated TiO/InS heterojunction as a promising multifunctional coating that combines infection control with osteogenic support, providing a potential strategy for improving the safety and longevity of Ti-based implants.
Immunoaffinity membrane for specific removal of hepatitis B virus from plasma.
He Z, He Y, Pan C … +5 more , Wang H, Fan Z, Shen Q, Shi Z, Jia L
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42330606 · Publisher ↗
The high global prevalence of hepatitis B virus (HBV) poses a significant threat to transfusion safety. In this study, we proposed a rationally designed immunoaffinity membrane for specific removal of HBV from plasma. A...
The high global prevalence of hepatitis B virus (HBV) poses a significant threat to transfusion safety. In this study, we proposed a rationally designed immunoaffinity membrane for specific removal of HBV from plasma. A single-chain variable fragment (scFv-1) targeting the HBV surface antigen (HBsAg) with high affinity was selected as the functional ligand for specific HBV capture. The scFv-1 was expressed in an E. coli Shuffle T7 system (yield: 125 ± 6 mg/L), purified to high monomeric purity, and covalently immobilized onto cellulose acetate membranes, obtaining the scFv-1 functionalized immunoaffinity membrane of CA-scFv-1. The CA-scFv-1 membrane exhibited Langmuir-type adsorption against HBsAg with a maximum static binding capacity of 10.3 mg/g (K = 2.11 ×10 mol/L). Under dynamic flow conditions, stacked CA-scFv-1 membranes (25 mm in diameter, 1.0 mm in thickness) achieved complete removal of HBsAg from plasma (20 mL, HBsAg concentration: 300 kIU/mL) at 100 L/m·h. Further study demonstrated that the CA-scFv-1 membrane could reduce HBV in clinical HBV-positive plasma effectively from 1 to 82 kIU/mL to undetectable levels, while normal plasma components and their biological function were well preserved. These results highlight the potential of our CA-scFv-1 immunoaffinity membrane in specific HBV removal for plasma purification.
Hydrogenated black TiO-based core-shell nanocarriers cloaked with erythrocyte membranes for thermal imaging-monitored chemo-photothermal therapy.
Li Y, Wang Y, Yu J … +8 more , Liu M, Mo Q, Zhao X, Chen Y, Liu W, Qin S, Zhang A, Cheng Y
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42330605 · Publisher ↗
Triple-negative breast cancer (TNBC) remains a major therapeutic challenge because of the lack of effective molecular targets, the limited efficacy of monotherapies, and the severe off-target toxicity associated with con...
Triple-negative breast cancer (TNBC) remains a major therapeutic challenge because of the lack of effective molecular targets, the limited efficacy of monotherapies, and the severe off-target toxicity associated with conventional chemotherapy. To address these limitations, we developed a biomimetic core-shell nanoplatform, denoted bT@M/D@P@R-DPF, for targeted chemo-photothermal therapy of TNBC. In this nanosystem, hydrogenated black TiO (bT) provides NIR photothermal conversion and thermal imaging capability; the disulfide-bridged mesoporous organosilica shell enables redox-responsive DOX release; PDA functions as a pH-sensitive gatekeeper and auxiliary photothermal component; and the DSPE-PEG-FA-engineered erythrocyte membrane (R-DPF) coating contributes to biomimetic shielding and folate receptor-mediated tumor targeting. The resulting nanoparticles (NPs) exhibited good colloidal stability, efficient photothermal conversion, pH/GSH-responsive drug release, and minimal premature leakage. In 4T1 cells, folate-functionalized NPs achieved markedly enhanced cellular uptake and cytotoxicity under combined chemo-photothermal treatment compared with non-targeted NPs. In 4T1 tumor-bearing mice, bT@M/D@P@R-DPF significantly increased tumor accumulation and vascular permeability, yielding a tumor inhibition rate of 91% under 808 nm laser irradiation without detectable acute systemic toxicity. In addition, the thermal imaging capability enabled real-time monitoring of tumor localization and therapeutic response. Overall, this study presents an integrated biomimetic chemo-photothermal nanoplatform that may provide a feasible and effective strategy for the multimodal treatment of TNBC.
Laser-programmable glycosaminoglycan-based nanocarriers co-deliver hypocrellin B and doxorubicin for spatiotemporal chemo-photothermal therapy of hepatocellular carcinoma.
Sohail M, Li K, Younas A … +7 more , Iqbal S, Chen X, Yang B, Shu G, Chen M, Yuan H, Ji J
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42322922 · Publisher ↗
Hepatocellular carcinoma (HCC) remains lethal due to systemic chemotherapy is halted by cardiotoxicity, multidrug resistance, and poor tumor penetration. To address these limitations, we developed a novel modified chondr...
Hepatocellular carcinoma (HCC) remains lethal due to systemic chemotherapy is halted by cardiotoxicity, multidrug resistance, and poor tumor penetration. To address these limitations, we developed a novel modified chondroitin sulfate/cystamine nanoparticle, termed CS-DHB NPs, that co-delivers doxorubicin (DOX) and the photosensitizer hypocrellin B (HB) and is encapsulated within a chondroitin sulfate/cystamine nanocarrier. These engineered nanoparticles (∼154 nm) demonstrated high drug-loading efficiencies (DOX: 80%; HB: 60%), exceptional serum stability (>72 h), and dual-drug release in laser- and pH-dependent environments. Upon 660 nm laser irradiation (1.0 W cm⁻²), the CS-DHB NPs produced substantial photothermal, photodynamic effects (¹O₂ quantum yield: 0.56), and excellent photostability. The prepared CS-DHB NPs were biocompatible and showed efficient in vitro cytotoxic effects via CCK-8 assays and deep penetration and inhibition of multicellular tumor spheroids (MTS). Ex vivo cellular studies revealed that the targeted delivery mechanism, clathrin-dependent endocytosis, with laser-triggered uptake, enhanced intracellular DOX accumulation twofold. In Hepa1-6 cells, CS-DHB NPs treatment induced pronounced apoptosis (∼51%). In vivo, five intravenous administrations combined with localized laser irradiation resulted in significant tumor regression, 100% survival, minimal systemic toxicity, and no histopathological damage in a Hepa1-6-bearing C57BL/6 mice model. By integrating chemotherapy, phototherapy, and targeted release within a tumor-targeted nanosystem, CS-DHB NPs offer a promising strategy to overcome chemoresistance while preserving healthy tissues, advancing an effective, and clinically translatable approach for synergistic HCC therapy.
Bio-catalytic nanofibers with sonodynamic therapy for rapid sterilization and enhanced infected wound healing.
He S, Chen Y, Shi H … +6 more , Chen L, He W, Zhou Y, Sun S, Peng L, Deng Y
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42320427 · Publisher ↗
Sonodynamic therapy (SDT) has been considered as a promising way to treat multidrug-resistant (MDR) bacterial infections. However, the efficiency of SDT is usually limited by rapid recombination of electron-hole pairs tr...
Sonodynamic therapy (SDT) has been considered as a promising way to treat multidrug-resistant (MDR) bacterial infections. However, the efficiency of SDT is usually limited by rapid recombination of electron-hole pairs triggered by ultrasound in sonosensitizers. To address this problem, we designed a new wound dressing composing lithium iron phosphate (LFP)/MXene (vanadium carbide) heterojunction (HJ). The constructed HJ exhibits high-efficient ROS production, Fe catalyzed ·OH generation while Fe oxidized consumption of GSH in bacteria. Furthermore, LFP is slowly degraded into non-toxic species including Li, Fe, and PO, while MXene is released from the composite. In vitro evaluations demonstrate that the composite membrane exhibits superior antibacterial performance against both S. aureus and E. coli through irreversible bacterial membrane disruption. Furthermore, in a full-thickness infected wound model, the engineered dressing significantly accelerates wound closure by eliminating infection. This study presents a viable paradigm for designing high-performance heterojunction-based fibrous scaffolds for the clinical management of infected wounds.
Nanozymes for caries and periodontitis: Mechanisms, therapeutic applications, and future perspectives.
Zhu H, Wang Y, Li Q … +3 more , Liu C, Deng J, Gao S
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42320426 · Publisher ↗
Dental caries and periodontitis are the most common chronic oral diseases, their occurrence and progression are closely linked to the complex oral microenvironment. These processes involve pathogenic microbial colonizati...
Dental caries and periodontitis are the most common chronic oral diseases, their occurrence and progression are closely linked to the complex oral microenvironment. These processes involve pathogenic microbial colonization, biofilm formation, inflammatory responses, and oxidative stress imbalance. Reactive oxygen species (ROS) exhibit dual biological roles in oral diseases: they participate in antimicrobial defense while potentially exacerbating tissue damage and inflammation when excessively accumulated, rendering precise redox homeostasis regulation a critical challenge in localized therapy. In recent years, nanozymes have demonstrated unique advantages in oral disease prevention and treatment due to their enzyme-mimicking catalytic functions, high stability, and structural tunability. This review first outlines the catalytic mechanisms of nanozymes and their roles in oral disease-related pathological processes, focusing on ROS regulation, antimicrobial activity, biofilm disruption, and oral microenvironment modulation. The structural classification and mechanisms of different nanozymes are subsequently summarized, including metals and metal compounds, carbon-based materials, and organic framework materials. Importantly, this review compares the applications of ROS-generating and ROS-scavenging nanozymes in dental caries and periodontitis, highlighting their therapeutic mechanisms and disease-specific design strategies. Furthermore, within specific disease contexts, this review examines representative nanozyme applications in caries prevention and periodontitis treatment, encompassing monotherapy and multimodal synergistic strategies. Particular attention is given to three emerging trends in oral nanozyme research, including microenvironment-responsive systems, multimodal therapeutic approaches, and clinical translation potential. Finally, this paper explores key challenges and future directions for the clinical translation of nanozymes in oral diseases, aiming to provide insights into material design and therapeutic optimization strategies.
Interfacial structural-functional modulation of PLA-TPGS macromolecular assemblies for synergistic Tri-modal colorectal cancer therapy.
Tran THT, Mai TTT, Phan KS … +7 more , Doan BT, Do HN, Nguyen TM, Bui TQ, Pham HN, Le TTH, Ha PT
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42314511 · Publisher ↗
The performance of a multifunctional nanotheranostic depends on how its macromolecular assembly controls the loaded agents at the interface. Here, we report a bio-based nanoplatform (M8) built from an amphiphilic poly(la...
The performance of a multifunctional nanotheranostic depends on how its macromolecular assembly controls the loaded agents at the interface. Here, we report a bio-based nanoplatform (M8) built from an amphiphilic poly(lactide)-TPGS matrix and designed for colorectal cancer theranostics. Beyond efficient co-encapsulation (>85%), the controlled assembly of these segments gives a stepwise structural amplification of transverse relaxivity (r), reaching 255.04 mM⋅s (r/r = 455.84). This 3.4-fold gain at 7 T, higher than common clinical iron-oxide agents, comes from local magnetic field inhomogeneities and slower water-proton diffusion. Molecular crowding and homo-FRET first lower the NIR emission inside the core, but this turns into a fluorescence dequenching effect that reveals tumour accumulation in CT26 colorectal cancer models. The lipophilic PPIX, owing to its lipophilicity, is expected to localize preferentially toward the polymer shell, supporting interfacial ROS generation; together with magnetic hyperthermia and doxorubicin chemotherapy, the platform delivers a triggered multimodal cytotoxic response. Under combined laser and alternating magnetic field, the cytotoxicity increased 13-fold for M8 (IC from 12.31 to 0.95 µg/mL) and 29-fold for M4 (from 3.51 to 0.12 µg/mL), while normal Vero cells stayed largely unaffected (IC 23-30 µg/mL), giving an in vitro therapeutic window above 30-fold. In CT26 tumour-bearing mice, M8 gave strong T2 signal darkening in the tumour, with 4.4- and 6-fold rises in the low- and high-signal pixel fractions, and NIR imaging confirmed tumour retention beyond 24 h. These results show how the colloidal structure links imaging and therapeutic performance, and offer a practical design route for colorectal cancer theranostics.
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