Bioinorganic Chemistry And Applications[JOURNAL]
Sun
200 papers
Plant Extract Based on Deep Eutectic Solvent-Mediated Biosynthesis of Silver Nanoparticles: Cytotoxicity and Antibacterial Effects.
Vorobyova VI
Bioinorg Chem Appl · 2023 · PMID 36691666 · Full text
Deep eutectic solvent DES-based grape pomace extracts (GPE) were used to synthesize silver nanoparticles (AgNPs) for the first time. This paper presents a synthesis of AgNPs by a novel method with GPE obtained by an eco-...
Deep eutectic solvent DES-based grape pomace extracts (GPE) were used to synthesize silver nanoparticles (AgNPs) for the first time. This paper presents a synthesis of AgNPs by a novel method with GPE obtained by an eco-friendly "green" solvent, namely, betaine-lactic acid and proline-lactic acid DESs. Compared with the water-based GPE, the DES-based grape pomace extracts contain lower reducing powers but additionally act as capping agent, which is the more important property for the creation of necessary particle nanosize and dispersion with colloidal stability. DESs were synthesized using a heating method, and Fourier transform infrared spectroscopy (FTIR) was carried out to confirm the formation of the DES. The phytochemical profile of GPE exhibits a high amount of hydroxycinnamic acids (23%), followed by anthocyanins (19%). The silver nanoparticles with a round shape were noticed on the scanning electron microscopy micrographs with the particle size in the range of 10-20 nm. The disc diffusion technique (DDT) showed that the AgNPs exhibited significant antibacterial activity against Gram-negative bacteria (. ) UKM В-906 and Gram-positivespore-forming (. ) UCМ В-506T. Mitotic index (MI) and chromosomal aberrations (CAs) were assessed by . assay. The synthesized silver nanoparticles do not induce cytogenotoxic and genotoxic changes in L. with nanoparticles at concentrations up to 10%.
The Strange Case: The Unsymmetric Cisplatin-Based Pt(IV) Prodrug [Pt(CHCOO)Cl(NH)(OH)] Exhibits Higher Cytotoxic Activity with respect to Its Symmetric Congeners due to Carrier-Mediated Cellular Uptake.
Gabano E, Zanellato I, Pinton G … +3 more , Moro L, Ravera M, Osella D
Bioinorg Chem Appl · 2022 · PMID 36620349 · Full text
The biological behavior of the axially unsymmetric antitumor prodrug (-6-44)-acetatodiamminedichloridohydroxidoplatinum(IV), , was deeply investigated and compared with that of analogous symmetric Pt(IV) complexes, namel...
The biological behavior of the axially unsymmetric antitumor prodrug (-6-44)-acetatodiamminedichloridohydroxidoplatinum(IV), , was deeply investigated and compared with that of analogous symmetric Pt(IV) complexes, namely, dihydroxido and diacetato , which have a similar structure. The complexes were tested on a panel of human tumor cell lines. Complex showed an anomalous higher cytotoxicity (similar to that of cisplatin) with respect to their analogues and . Their reduction potentials, reduction kinetics, lipophilicity, and membrane affinity are compared. Cellular uptake and DNA platination of Pt(IV) complexes were deeply investigated in the sensitive A2780 human ovarian cancer cell line and in the corresponding resistant A2780cisR subline. The unexpected activity of appears to be related to its peculiar cellular accumulation and not to a different rate of reduction or a different efficacy in DNA platination and/or efficiency in apoptosis induction. Although the exact mechanism of cell uptake is not fully deciphered, a series of naïve experiments indicates an energy-dependent, carrier-mediated transport: the organic cation transporters (OCTs) are the likely proteins involved.
Glutaraldehyde-Polymerized Hemerythrin: Evaluation of Performance as an Oxygen Carrier in Hemorrhage Models.
Stoica AD, Toma VA, Roman I … +3 more , Sevastre B, Scurtu F, Silaghi-Dumitrescu R
Bioinorg Chem Appl · 2022 · PMID 36620348 · Full text
Hemoglobin-based oxygen carriers (HBOCs) have been proposed and tested for several decades for the treatment of hemorrhage. We have previously proposed replacing hemoglobin (Hb) in HBOC with the oxygen-carrying protein h...
Hemoglobin-based oxygen carriers (HBOCs) have been proposed and tested for several decades for the treatment of hemorrhage. We have previously proposed replacing hemoglobin (Hb) in HBOC with the oxygen-carrying protein hemerythrin (Hr), from marine worms, showing that Hr-based derivatives can perform at least as well or even better than Hb-based HBOC in a range of in vitro assays involving oxidative and nitrosative stress as well as in top-up animal models, where small amounts of Hr- or Hb-HBOC were injected into rats. Here, these experiments are extended to a hemorrhage experiment, in which Hr polymerized with glutaraldehyde, alone or conjugated with human serum albumin, is administered after a loss of 20-30% blood volume. The performance of these preparations is compared with that of Hb-based HBOC measured under the same conditions. Polymerized Hr is found to decrease the survival rate and can hence cannot be used as an oxygen carrier in transfusions. On the other hand, an Hr-albumin copolymer restores survival rates to 100% and generally yields biochemical and histological parameters similar to those of glutaraldehyde-polymerized bovine hemoglobin, with the exception of an acid-base imbalance. The latter may be solved by employing an allogeneic albumin as opposed to the human albumin employed in the present study.
Targeting Tumor Microenvironment by Metal Peroxide Nanoparticles in Cancer Therapy.
Mbugua SN
Bioinorg Chem Appl · 2022 · PMID 36568636 · Full text
Solid tumors have a unique tumor microenvironment (TME), which includes hypoxia, low acidity, and high hydrogen peroxide and glutathione (GSH) levels, among others. These unique factors, which offer favourable microenvir...
Solid tumors have a unique tumor microenvironment (TME), which includes hypoxia, low acidity, and high hydrogen peroxide and glutathione (GSH) levels, among others. These unique factors, which offer favourable microenvironments and nourishment for tumor development and spread, also serve as a gateway for specific and successful cancer therapies. A good example is metal peroxide structures which have been synthesized and utilized to enhance oxygen supply and they have shown great promise in the alleviation of hypoxia. In a hypoxic environment, certain oxygen-dependent treatments such as photodynamic therapy and radiotherapy fail to respond and therefore modulating the hypoxic tumor microenvironment has been found to enhance the antitumor impact of certain drugs. Under acidic environments, the hydrogen peroxide produced by the reaction of metal peroxides with water not only induces oxidative stress but also produces additional oxygen. This is achieved since hydrogen peroxide acts as a reactive substrate for molecules such as catalyse enzymes, alleviating tumor hypoxia observed in the tumor microenvironment. Metal ions released in the process can also offer distinct bioactivity in their own right. Metal peroxides used in anticancer therapy are a rapidly evolving field, and there is good evidence that they are a good option for regulating the tumor microenvironment in cancer therapy. In this regard, the synthesis and mechanisms behind the successful application of metal peroxides to specifically target the tumor microenvironment are highlighted in this review. Various characteristics of TME such as angiogenesis, inflammation, hypoxia, acidity levels, and metal ion homeostasis are addressed in this regard, together with certain forms of synergistic combination treatments.
Antimicrobial Effects of Gum Arabic-Silver Nanoparticles against Oral Pathogens.
Ahmed O, Sibuyi NRS, Fadaka AO … +5 more , Madiehe AM, Maboza E, Olivier A, Meyer M, Geerts G
Bioinorg Chem Appl · 2022 · PMID 36561898 · Full text
Dental caries is considered one of the most prevalent oral diseases worldwide, with a high rate of morbidity among populations. It is a chronic infectious disease with a multifactorial etiology that leads to the destruct...
Dental caries is considered one of the most prevalent oral diseases worldwide, with a high rate of morbidity among populations. It is a chronic infectious disease with a multifactorial etiology that leads to the destruction of the dental tissues. Due to their antimicrobial, anti-inflammatory, antifungal, and antioxidant properties; silver nanoparticles (AgNPs) are incorporated in dental products to help prevent infectious oral diseases. In this study, the antimicrobial effects of AgNPs synthesized using Gum Arabic extracts (GAE) were examined. The GA-AgNPs were synthesized and characterized using ultraviolet-visible (UV-Vis) spectrophotometer, dynamic light scattering (DLS), transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. The antimicrobial activity of the GA-AgNPs was evaluated on (. ), (. ), (. ), and (. ) using agar disc diffusion and microdilution assays. The antibiofilm of GA-AgNPs was evaluated on the surface of human tooth enamel that had been exposed to . with and without the GA-AgNPs using scanning electron microscopy (SEM). GA-AgNPs were spherical in shape with a particle size distribution between 4 and 26 nm. The GA-AgNPs exhibited antimicrobial activity against all the tested oral microbes, with GA-AgNPs_0.4g having higher antimicrobial activity. The GA-AgNPs_0.4g inhibited . adhesion and biofilm formation on the surface of the tooth enamel. Therefore, this study supports the prospective implementation of the plant extract-mediated AgNPs in dental healthcare.
Comprehensive Assessment of Biomolecular Interactions of Morpholine-Based Mixed Ligand Cu(II) and Zn(II) Complexes of 2,2'-Bipyridine as Potential Anticancer and SARS-CoV-2 Agents: A Synergistic Experimental and Structure-Based Virtual Screening.
Sakthikumar K, Krause RWM, Isamura BK
Bioinorg Chem Appl · 2022 · PMID 36545430 · Full text
A new class of pharmacologically active mixed-ligand complexes (-) [M(L) (bpy)], where = 2-(4-morpholinobenzylideneamino)phenol), bpy = 2,2'-bipyridine, M = Cu (), and Zn (), were assigned an octahedral geometry by anal...
A new class of pharmacologically active mixed-ligand complexes (-) [M(L) (bpy)], where = 2-(4-morpholinobenzylideneamino)phenol), bpy = 2,2'-bipyridine, M = Cu (), and Zn (), were assigned an octahedral geometry by analytical and spectral measurements. Gel electrophoresis showed that complex () demonstrated the complete DNA cleavage mediated by HO. The overall DNA-binding constants observed from UV-vis, fluorometric, hydrodynamic, and electrochemical titrations were in the following sequence: () > () > (), which suggests that the complexes might intercalate DNA, a possibility that is further supported by the biothermodynamic characteristics. The binding constant results of BSA by electronic absorption and fluorometric titration demonstrate that complex () exhibits the highest binding effectiveness among others, which means that all compounds could interact with BSA through a static approach, additionally supported by FRET measurements. Density FunctionalTheory (DFT) and molecular docking calculations were relied on to unveil the electronic structure, reactivity, and interacting capability of all substances with DNA, BSA, and SARS-CoV-2 main protease (Mpro). These observed binding energies fell within the following ranges: -7.7 to -8.6, -7.2 to -10.2, and -6.7 to -8.2 kcal/mol, respectively. The higher reactivity of the complexes compared to free ligand is supported by the Frontier MolecularOrbital (FMO) theory. The antibacterial, cytotoxic, and radical scavenging characteristics revealed that complex () has the best biological efficacy compared to others. This is encouraged because all experimental findings are closely correlated with the theoretical measurements.
Deferiprone-Resveratrol Hybrid, an Iron-Chelating Compound, Acts as an Antimalarial and Hepatoprotective Agent in -Infected Mice.
Chuljerm H, Maneekesorn S, Punsawad C … +5 more , Somsak V, Ma Y, Ruangsuriya J, Srichairatanakool S, Koonyosying P
Bioinorg Chem Appl · 2022 · PMID 36466999 · Full text
Free heme in plasma acts as a prooxidant; thus, it is bound to hemopexin and eliminated by the liver. High iron content in the liver can support growth and cause oxidative liver injury. Inversely, the withholding of exc...
Free heme in plasma acts as a prooxidant; thus, it is bound to hemopexin and eliminated by the liver. High iron content in the liver can support growth and cause oxidative liver injury. Inversely, the withholding of excessive iron can inhibit this growth and protect the liver against malaria infection. This study examined the effects of a deferiprone-resveratrol (DFP-RVT) hybrid on malaria parasites and its relevant hepatoprotective properties. Mice were infected with , gavage DFP-RVT, deferiprone (DFP), and pyrimethamine (PYR) for 8 consecutive days. Blood and liver parameters were then evaluated. The presence of blood-stage parasites was determined using the microscopic Giemsa staining method. Subsequently, plasma liver enzymes, heme, and concentrations of thiobarbituric acid-reactive substances (TBARS) were determined. The liver tissue was examined pathologically and heme and TBARS concentrations were then quantified. The results indicate that the suppression potency against growth occurred as follows: PYR > DFP-RVT hybrid > DFP. Importantly, DFP-RVT significantly improved RBC size, restored alanine aminotransferase and alkaline activities, and increased heme and TBARS concentrations. The compound also reduced the liver weight index, heme, and TBARS concentrations significantly when compared to mice that were untreated. Our findings support the contention that the hepatoprotective effect of DFP-RVT is associated with parasite burden, iron depletion, and lipid peroxidation in the host.
Evaluation of Behavior of 13X Zeolite Modified with Transition Metals for Catalytic Applications.
David E
Bioinorg Chem Appl · 2022 · PMID 36340969 · Full text
This work was intended to develop catalysts based on 13X zeolite modified with transition metals for catalytic applications. In this regard, 13X zeolite was modified by loading of transition metals such as Fe, Co, Cu and...
This work was intended to develop catalysts based on 13X zeolite modified with transition metals for catalytic applications. In this regard, 13X zeolite was modified by loading of transition metals such as Fe, Co, Cu and various types of catalysts such as Fe-, Co-, Cu-, Fe-Co-, Fe-Cu-, and Co-Cu/13X zeolite were obtained. To prepare these catalysts, the wet impregnation method and metallic precursors were used. The catalysts were characterized by SEM, XRD, BET, and ammonia adsorption. Then the catalytic performance was investigated during upgrading of rapeseed residual biomass pyrolysis vapors using this catalysts and a fixed-bed reactor in two stages. Experimental results showed that the addition of transition metals improved the catalytic selectivity towards aromatic hydrocarbons and Fe-Cu/13X zeolite catalyst was the best and had a high deoxygenation activity (from 62.45% to 20.56%), produced maximum monoaromatic hydrocarbons (of 27.45%), the oxygen content in bio-oil was reduced from 34.98 wt% to 16.06 wt%, the calorific value increased and thus the bio-oil quality was improved.
Molecular Docking and Green Synthesis of Bioinorganic TiO Nanoparticles against and .
Al-Serwi RH, El-Sherbiny M, Kumar TVA … +7 more , Qasim AA, Khattar T, Alghazwani Y, Alqahtani A, Krishnaraju V, Muthu Mohamed JM, Sundramurthy VP
Bioinorg Chem Appl · 2022 · PMID 36285040 · Full text
This study used a simple solution evaporation approach to make a bioinorganic titanium dioxide (Bi-TiO) photocatalyst for dye contaminant degradation. A variety of techniques, including -ray diffraction (XRD), Fourier-tr...
This study used a simple solution evaporation approach to make a bioinorganic titanium dioxide (Bi-TiO) photocatalyst for dye contaminant degradation. A variety of techniques, including -ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) coupled with energy dispersive -ray analysis (EDAX), and differential reflectance spectroscopy, had been employed to classify the structural and optical properties of the prepared bioinorganic photocatalyst (UV-DRS). Using simulated solar irradiation, the photocatalytic activity of the produced Bi-TiO nanoparticles was examined by detecting the degradation of a solution of methylene blue (MB) as a model dye molecule. The developed Bi-TiO photocatalyst demonstrates superior photocatalytic action than commercially available powder TiO, according to photo-degradation experiments. E. and S. bacterial strains were employed to assess the antibacterial activity of Bi-TiO nanoparticles. The most active molecules that gain antibacterial activity were examined in isolated or extracted components from the tulsi plant. The chosen compounds were docked with thymidylate kinase (TMPK), a potential therapeutic goal for the preparation of novel antibacterial drugs with the PDB ID of 4QGG. Five compounds, namely rosmarinic acid, vicenin-2, orientin, vitexin, and isoorientin, out of the 27 chosen compounds, showed a higher docking score and may aid in boosting antibacterial activity. The synthesized Bi-TiO nanoparticles produced antibacterial activity that was effective against Gram-positive bacteria. The nanomaterials that have been synthesized have a lot of potential in wastewater treatment and biomedical management technologies.
Facile Fabrication of CuO Nanoparticles Embedded in N-Doped Carbon Nanostructure for Electrochemical Sensing of Dopamine.
Sobahi N, Imran M, Khan ME … +7 more , Mohammad A, Alam MM, Yoon T, M Mehedi I, Hussain MA, Abdulaal MJ, A Jiman A
Bioinorg Chem Appl · 2022 · PMID 36276988 · Full text
In the present study, a highly selective and sensitive electrochemical sensing platform for the detection of dopamine was developed with CuO nanoparticles embedded in N-doped carbon nanostructure (CuO@NDC). The successfu...
In the present study, a highly selective and sensitive electrochemical sensing platform for the detection of dopamine was developed with CuO nanoparticles embedded in N-doped carbon nanostructure (CuO@NDC). The successfully fabricated nanostructures were characterized by standard instrumentation techniques. The fabricated CuO@NDC nanostructures were used for the development of dopamine electrochemical sensor. The reaction mechanism of a dopamine on the electrode surface is a three-electron three-proton process. The proposed sensor's performance was shown to be superior to several recently reported investigations. Under optimized conditions, the linear equation for detecting dopamine by differential pulse voltammetry is (A) = 0.07701 (M) - 0.1232 ( = 0.996), and the linear range is 5-75 M. The limit of detection (LOD) and sensitivity were calculated as 0.868 M and 421.1 A/M, respectively. The sensor has simple preparation, low cost, high sensitivity, good stability, and good reproducibility.
The Adsorption Potential of Cr from Water by ZnO Nanoparticles Synthesized by .
Wenjie O, Ahmed W, Xiuxian F … +9 more , Lu W, Jiannan L, Jie Y, Asghar RMA, Mahmood M, Alatalo JM, Imtiaz M, Li W, Mehmood S
Bioinorg Chem Appl · 2022 · PMID 36268517 · Full text
Aqueous solutions containing toxic elements (TEs) (such as hexavalent chromium (Cr (VI)) can be toxic to humans even at trace levels. Thus, removing TEs from the aqueous environment is essential for the protection of bio...
Aqueous solutions containing toxic elements (TEs) (such as hexavalent chromium (Cr (VI)) can be toxic to humans even at trace levels. Thus, removing TEs from the aqueous environment is essential for the protection of biodiversity, hydrosphere ecosystems, and humans. For plant fabrication of zinc oxide nanoparticles (PF-ZnONPs), plants were used, and X-ray diffraction (XRD), energy dispersive spectroscopy (EDS), SEM, and FTIR techniques were used for the identification of PF-ZnONPs and ZnONPs, which were used to remove Cr (VI) from aqueous solution. A number of adsorption parameters were studied, including pH, dose, concentration of metal ions, and contact time. The removal efficiency of PF-ZnONPs for Cr (VI) has been found to be 96% at a time (60 min), 69.02% at pH 4, and 70.43% at a dose (10 mg·L). It was found that the pseudo-second-order model best described the adsorption of Cr (VI) onto PF-ZnONPs, indicating a fast initial adsorption via diffusion. The experimental data were also highly consistent with the Langmuir isotherm model calculations.
Research on the Purification of Environmental Pollution by Biomaterials and Its Treatment Methods in the Development of Rural Cultural and Creative Industries.
Fan T, Fu T, Xu Q … +1 more , Gjoni G
Bioinorg Chem Appl · 2022 · PMID 36248628 · Full text
Since our country put forward the rural revitalization strategy, we have attached great importance to the construction and development of urban and rural areas. In recent years, we have continuously enhanced the driving...
Since our country put forward the rural revitalization strategy, we have attached great importance to the construction and development of urban and rural areas. In recent years, we have continuously enhanced the driving force of development and further promoted rural reform. However, in the construction of rural cultural industry, how to purify environmental pollution is a key and difficult point. Through the comprehensive analysis of the role and effect of biomaterials in environmental pollution purification, this study compares the comprehensive role of different materials in environmental purification, compares the trend of environmental pollution in the development of rural industries in recent years, and analyzes the coupling degree of environmental pollution purification in different technological environments. Through the analysis, we can draw a conclusion that under the action of nanomaterials and biomaterials, some water pollution, air pollution, construction waste pollution, and noise pollution in industrial development have achieved good results and impact. Through continuous practice and research, it can be seen that biotechnology and materials can play a relatively good role in the environmental pollution control. And this treatment technology has the advantages of low cost, high efficiency, and no secondary pollution. It also plays an important role in the treatment of pollutants and the development of new energy. It conforms to the concept of sustainable development and environmental construction in the modern society and provides more reference data and research value for the future research and development prospects of nanomaterials and biomaterials in industrial development and environmental governance.
Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer.
Abdellatif AAH, Abdelfattah A, Bouazzaoui A … +8 more , Osman SK, Al-Moraya IS, Showail AMS, Alsharidah M, Aboelela A, Al Rugaie O, Faris TM, Tawfeek HM
Bioinorg Chem Appl · 2022 · PMID 36248627 · Full text
Tumor necrosis factor (TNF-) and inflammatory cytokine (IL-6) play a vital role in various cellular incidents such as the proliferation and death of cells during carcinogenesis. Hence, regulation of these biomarkers coul...
Tumor necrosis factor (TNF-) and inflammatory cytokine (IL-6) play a vital role in various cellular incidents such as the proliferation and death of cells during carcinogenesis. Hence, regulation of these biomarkers could be a promising tool for controlling tumor progression using nanoformulations. Silver nanoparticles-poly (vinyl pyrrolidone) (AgNPs-PVP) were prepared using the reduction of silver nitrate and stabilized with PVP. They are characterized through yield percentage, UV-VIS, FT-IR, size, charge, and morphology. The obtained AgNPs were tested for anticancer activity against prostate cancer (PC 3) and human skin fibroblast (HFS) cell lines. Moreover, biomarker-based confirmations like TNF- and IL-6 were estimated. The synthesized AgNPs-PVP were stable, spherical in shape, with particle sizes of 122.33 ± 17.61 nm, a polydispersity index of 0.49 ± 0.07, and a negative surface charge of -19.23 ± 0.61 mV. cytotoxicity testing showed the AgNPs-PVP exhibited antiproliferation properties in PC3 in a dose-dependent manner. In addition, when compared to control cells, AgNPs-PVP has lower TNF- with a significant value ( < 0.05); the value reached 16.84 ± 0.71 pg/ml versus 20.81 ± 0.44 pg/ml, respectively. In addition, HSF cells showed a high level of reduction ( < 0.001) in IL-6 production. This study suggested that AgNPs-PVP could be a possible therapeutic agent for human prostate cancer and anti-IL-6 in cancerous and noncancerous cells. Further studies will be performed to investigate the effect of AgNPs-PVP in different types of cancer.
Research on Microseismic Source Location Method Based on Waveform Characteristics Monitored by Nanomaterial Sensor under the Background of Metal Oxide Polluted Environment.
Wang S, Hu Z, Lu S
Bioinorg Chem Appl · 2022 · PMID 36248626 · Full text
In recent years, the monitoring range of source location technology has developed from being one-dimensional and two-dimensional to being three-dimensional. However, due to the complexity and nonuniformity of the seismic...
In recent years, the monitoring range of source location technology has developed from being one-dimensional and two-dimensional to being three-dimensional. However, due to the complexity and nonuniformity of the seismic wave propagation medium and the uncertainty of the propagation law, there will be large errors in the source location results. Therefore, the analysis of vibration signal has become the key problem of current research. This paper designs a microseismic monitoring system based on Internet of Things sensors, which can monitor the vibration wave characteristics of vibration signals. In order to test the positioning accuracy of the system, this paper introduces three positioning methods: target positioning method based on time difference, time delay estimation method based on EMD, and source target positioning method based on the characteristic frequency of vibration signal. The purpose of this paper is to find the most accurate method from the three source location methods. Through these three methods, the vibration source generated by a single person walking in situ can be located in the vibration positioning experiment of human walking. The error between the actual position and the measurement source position is compared. The results show that the time delay estimation method based on empirical mode decomposition has the highest positioning accuracy. In addition, in the microseismic experiment, it is proved that the positioning accuracy of EMD using L1 norm statistical criterion is higher than that using L2 norm statistical criterion.
Synthesis of Zinc Oxide (ZnO)-Titanium Dioxide (TiO)-Chitosan-Farnesol Nanocomposites and Assessment of Their Anticancer Potential in Human Leukemic MOLT-4 Cell Line.
Elderdery AY, Alzahrani B, Hamza SMA … +3 more , Mostafa-Hedeab G, Mok PL, Subbiah SK
Bioinorg Chem Appl · 2022 · PMID 36212987 · Full text
Leukemia is the most prevalent cancer in children and one of the most common and deadly cancers that affect adults. Several metal oxide nanoparticles, biopolymers, and phytochemicals have been discovered to target cancer...
Leukemia is the most prevalent cancer in children and one of the most common and deadly cancers that affect adults. Several metal oxide nanoparticles, biopolymers, and phytochemicals have been discovered to target cancer cells selectively while inflicting low to no damage to healthy cells. Among the existing nanoparticle synthesis methodologies, biologically synthesized nanoparticles using phytochemicals have emerged as a straightforward, economical, and environmentally sound strategy. The synergistic antitumor potential of ZnO-TiO-chitosan-farnesol nanocomposites (NCs) against leukemia MOLT-4 cells was investigated in the current study. After synthesizing the NCs, characterization of the same was carried out using XRD, DLS, FESEM, TEM, PL, EDX, and FTIR spectroscopy. To analyze its anticancer activity, MOLT-4 cells were cultured and treated at diverse dosages of NCs. The cell viability upon treatment was examined by MTT assay. The morphological and nuclear modifications were observed by dual staining. ROS and MMP levels were observed by DCFH-DA staining and Rh-123 dye, respectively. Furthermore, the caspase 3, 8, and 9 levels were examined by performing ELISA. The XRD patterns exhibited a hexagonal structure of the NCs. In the DLS spectrum, the hydrodynamic diameter of the NCs was observed to be 126.2 nm. The electrostatic interface between the ZnO-TiO-chitosan-farnesol NCs was confirmed by the FTIR spectra. A significant loss of cell viability in a dosage-dependent trend confirmed the cytotoxic effect of the NCs. An elevated ROS level and MMP depletion suggested apoptosis-associated cell death via the intrinsic pathway, which was confirmed by elevated expressions of caspase 3, 8, and 9 markers. Thus, the results showed that the synthesized NCs demonstrated a remarkable anticancer potential against leukemic cells and can be potentially valuable in cancer treatments. The findings from this study conclude that this is a new approach for modifying the physicochemical characteristics of ZnO-TiO-chitosan-farnesol composites to increase their properties and synergistically exhibit anticancer properties in human leukemic cancer cells.
Structural, Optical, Antibacterial, and Anticancer Properties of Cerium Oxide Nanoparticles Prepared by Green Synthesis Using Leaves Extract.
Elderdery AY, Alzahrani B, Alabdulsalam AA … +7 more , Hamza SMA, Elkhalifa AME, Alhamidi AH, Alanazi F, Mohamedain A, Subbiah SK, Ling Mok P
Bioinorg Chem Appl · 2022 · PMID 36212986 · Full text
Currently, new advancements in the area of nanotechnology opened up new prospects in the field of medicine that could provide us with a solution for numerous medical complications. Although a several varieties of nanopar...
Currently, new advancements in the area of nanotechnology opened up new prospects in the field of medicine that could provide us with a solution for numerous medical complications. Although a several varieties of nanoparticles is being explored to be used as nanomedicines, cerium oxide nanoparticles (CeO NPs) are the most attractive due to their biocompatibility and their switchable oxidation state (+3 and +4) or in other words the ability to act as prooxidant and antioxidant depending on the pH condition. Green synthesis of nanoparticles is preferred to make it more economical, eco-friendly, and less toxic. The aim of our study here is to formulate the CeO NPs (CeO NPs) using (Noni) leaf extract and study its optical, structural, antibacterial, and anticancer abilities. Their optical and structural characterization was accomplished by employing X-ray diffractography (XRD), TEM, EDAX, FTIR, UV-vis, and photoluminescence assays. Our CeO NPs expressed strong antibacterial effects against Gram-positive and in addition to Gram-negative and when compared with amoxicillin. The anticancer properties of the green synthesized CeO NPs against human acute lymphoblastic leukemia (ALL) MOLT-4 cells were further explored by the meticulous study of their ability to diminish cancer cell viability (cytotoxicity), accelerate apoptosis, escalate intracellular reactive oxygen species (ROS) accumulation, decline the mitochondria membrane potential (MMP) level, modify the cell adhesion, and shoot up the activation of proapoptotic markers, caspase-3, -8, and -9, in the tumor cells. Altogether, the outcomes demonstrated that our green synthesized CeO NPs are an excellent candidate for alternative cancer therapy.
Study on Nano Drug Particles in the Diagnosis and Treatment of Alzheimer's Disease in the Elderly.
Li C, Xiang Y, Wang Y … +1 more , Li P
Bioinorg Chem Appl · 2022 · PMID 36212985 · Full text
OBJECTIVE: To study the clinical effect of nano drug particles in the diagnosis and treatment of Alzheimer's disease in the elderly, we promote the research on the treatment of Alzheimer's disease and provide basis. METH...
OBJECTIVE: To study the clinical effect of nano drug particles in the diagnosis and treatment of Alzheimer's disease in the elderly, we promote the research on the treatment of Alzheimer's disease and provide basis. METHODS: 80 patients with Alzheimer's disease treated in our hospital from April 2021 to April 2022 were selected as the research objects and were divided into the reference group and the observation group by random number table method. The reference group was given traditional treatment drugs, and the observation group was given nano drug particles. The treatment effect, blood lipid level, cognitive function status, and comprehensive effective rate of the two groups were compared, and the treatment data of the patients were scored by ADL and MMSE scale to understand the intelligence level and daily living ability of the patients. RESULTS: From the comparison results, it is found that the blood lipid level of the control group was in the normal range. The cognitive function of the control group was also better than that of the reference group. The scores of ADL and MMSE in the control group were higher than those in the reference group. The effective rate of the control group was also higher than that of the reference group; < 0.05, with statistical significance. CONCLUSION: In the diagnosis and treatment of Alzheimer's disease in the elderly, nano drug particles have good stability and less toxic and side effects, improve the ability of daily living of patients, and have good clinical treatment effect, which is worthy of clinical application.
Synthesis, Characterization, and Antiproliferative Effect of CuO-TiO-Chitosan-Amygdalin Nanocomposites in Human Leukemic MOLT4 Cells.
Elderdery AY, Alzahrani B, Hamza SMA … +3 more , Mostafa-Hedeab G, Mok PL, Subbiah SK
Bioinorg Chem Appl · 2022 · PMID 36199748 · Full text
The main aim of this study was to synthesize copper oxide- (CuO-) titanium oxide- (TiO-) chitosan-amygdalin nanocomposites (CTCANc) and to characterize them physically and biologically (antimicrobial and anticancer activ...
The main aim of this study was to synthesize copper oxide- (CuO-) titanium oxide- (TiO-) chitosan-amygdalin nanocomposites (CTCANc) and to characterize them physically and biologically (antimicrobial and anticancer activity using MOLT4 blood cancer cell line) to endorse their useful applications as potential drug candidates in anticancer avenues. CuO-TiO-chitosan-amygdalin nanocomposites were synthesized according to standard, reported methods. Physical characterization of the nanocomposites was performed using methods like X-ray diffractometer (XRD), and morphological and ultrastructural analysis of nanocomposites were done using electron microscope scanning and transmission. FTIR was recorded using a Perkin-Elmer spectrometer, and photoluminescence (PL) spectra were done using the spectrometer. Further, antibacterial activities were assessed using standard bacterial cultures. To demonstrate the nanocomposite's anticancer effects, MTT assay, morphological analysis, apoptosis studies using acridine orange/ethidium bromide (AO/EtBr) dual staining, reactive oxygen species (ROS) analysis, and levels of antioxidant enzymes were analyzed using the MOLT4 blood cancer cell line. Synthesized nanocomposites were characterized using XRD and showed various peaks, respectively, for CuO-TiO, amygdalin, and chitosan. MTT assay indicated an IC value of 38.41 g/ml concentration of CTCANc. Hence, 30 and 40 g/ml were used for the subsequent experiments. Morphological analysis, staining for apoptosis using AO/EtBr, mitochondrial membrane potential (MMP or ΔΨm) analysis, ROS analysis, and determination of the SOD, CAT, MDA, and GSH levels were performed. Observations like a significant loss of morphology, induction of apoptosis, elevated ROS, and decreased MMP were significant in 30 and 40 g/ml nanocomposite-treated cells when compared to control cells. The bimetallic nanocomposites exhibited typical nanocomposites characteristics and significant antibacterial and anticancer effects. The study results endorse the antibacterial, anticancer activity of CuO-TiO-chitosan-amygdalin nanocomposites and strongly suggest that further in-depth research using CuO-TiO-chitosan-amygdalin nanocomposites could reveal their efficacy in the clinical scenario.
Fungal- and Algal-Derived Synthesis of Various Nanoparticles and Their Applications.
Michael A, Singh A, Roy A … +1 more , Islam MR
Bioinorg Chem Appl · 2022 · PMID 36199747 · Full text
Nanoparticles synthesis through biological mediated methods with a particular focus on the processes mediated by fungi and algae is discussed, which systematically reviews nanoparticle characterization, composition, synt...
Nanoparticles synthesis through biological mediated methods with a particular focus on the processes mediated by fungi and algae is discussed, which systematically reviews nanoparticle characterization, composition, synthesis methods, and, lastly but not least, the applications of NPs across five different categories to provide a reference for future research. Most traditional methods to generate nanoparticles have certain limitations, like the toxicity of precursor materials, the need for high-temperature management, and the high cost of synthesis, which ultimately hinders their utility in sectors. Greener synthesis through fungus and algae done through bioreduction by biomolecules or enzymes present in them is low-energy, low-cost, and needs a low-temperature environment, providing a unique technique for the manufacture of various metallic nanoparticles utilized in an array of industries and healthcare.
Cytotoxicity and Genotoxicity of Biogenic Silver Nanoparticles in A549 and BEAS-2B Cell Lines.
Muhamad M, Ab Rahim N, Wan Omar WA … +1 more , Nik Mohamed Kamal NNS
Bioinorg Chem Appl · 2022 · PMID 36193250 · Full text
INTRODUCTION: Biogenic silver nanoparticles (AgNPs-GA) were successfully synthesised using leaf extract as a reducing agent, which has ethnopharmacological claims against various diseases including cancer. . Aim of the...
INTRODUCTION: Biogenic silver nanoparticles (AgNPs-GA) were successfully synthesised using leaf extract as a reducing agent, which has ethnopharmacological claims against various diseases including cancer. . Aim of the study is to discover whether AgNPs-GA has cytotoxic and genotoxic effects on cancerous (A549) and noncancerous (BEAS-2B) human lung cells. MATERIALS AND METHODS: The cytotoxicity profiles of AgNPs-GA were characterized by MTT assay, intracellular reactive oxygen species (ROS) assay, and DAPI and AOPI double staining, whilst genotoxicity was assessed using Comet Assay analysis. The level of silver ions (Ag) and cellular uptake of AgNPs-GA were evaluated by ICP-OES and TEM analyses, respectively. RESULTS: A significant cytotoxic effect was observed by AgNPs-GA on both A549 and BEAS-2B cell lines, with IC values of 20-28 g/ml and 12-35 g/ml, respectively. The cytotoxicity profile of AgNPs-GA was also accompanied by a pronounced increase in ROS production, DNA damage, and apoptosis. Moreover, Ag was also detected in cells exposed to AgNPs-GA threefold higher compared to controls. In this study, AgNPs-GA were endocytosed within lysosomes, which may direct to secondary toxicity effects including oxidative stress, impairment of the cell membrane, DNA fragmentation, and cell death. CONCLUSIONS: Taken together, novel toxicological-related mechanisms by AgNPs-GA were proposed involving the generation of ROS that causes DNA damage which led to programmed cell death in both A549 and BEAS-2B cells. Therefore, a combination of scientific assessments is constantly needed to ensure that the quality of biosynthesized nanoparticles is controlled and their safe development is promoted.
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