Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia[JOURNAL]
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200 papers
[Genome organization and life cycle of the hepatitis c virus].
Kalinina OV, Dmitriev AV
Mol Gen Mikrobiol Virusol · 2015 · PMID 26182661
The review summarizes the current data about the hepatitis C viral genome and polyprotein organization. The functional role of the structural and non-structural viral proteins including their interaction with cellular re...
The review summarizes the current data about the hepatitis C viral genome and polyprotein organization. The functional role of the structural and non-structural viral proteins including their interaction with cellular regulatory proteins and cell structural elements is discussed. Specific peculiarities of the life cycle of the hepatitis C virus important for the understanding of the viral hepatitis C pathogenesis are summarized.
[Maldi-tof ms analysis for yersinia pestis, vibrio cholera, and francisella tularensis identification].
Afanas'ev MV, Mironova LV, Balakhonov SV
Mol Gen Mikrobiol Virusol · 2015 · PMID 26182660
Numerous studies showed that a new technology for the clinical microbiology laboratories, Matrix-Assisted Laser Desorption Ionization--Time of Flight Mass Spectrometry (MALDI-ToF MS), allows fast, accurate, and effective...
Numerous studies showed that a new technology for the clinical microbiology laboratories, Matrix-Assisted Laser Desorption Ionization--Time of Flight Mass Spectrometry (MALDI-ToF MS), allows fast, accurate, and effective identification of most clinically relevant microorganisms to be implemented. In the present review, we discuss applications of this approach for identification and typing of extremely dangerous pathogens--Yersinia pestis, Vibrio cholera, and Francisella tularensis, including the advantages and disadvantages of the method, sample preparation and biosafety problems.
[TULA HANTAVIRUS IN CRIMEA].
Yashina LN, Zaykovskaya AV, Protopopova E K … +4 more , Babkin IV, Malyshev BS, Tovpinets NN, Evstafiev IL
Mol Gen Mikrobiol Virusol · 2015 · PMID 27192820
Genetic evidence of the Tula virus (TULV) in Crimea region of Russia is presented. Based on the reverse transcription PCR and subsequent sequence analysis, a total of 4 RNA isolates of the TULV were identified from the t...
Genetic evidence of the Tula virus (TULV) in Crimea region of Russia is presented. Based on the reverse transcription PCR and subsequent sequence analysis, a total of 4 RNA isolates of the TULV were identified from the tissue samples of the Altai voles Microtus obscurus captured in the Bakhchisaray district of the Republic Crimea. Phylogenetic analysis of the S-, M-, and L-segment sequences of the Crimean TULV strains showed that they formed distinct genetic lineage, Russia IV, in the TULV variant. New sequences were most closely related to the lineage Russia I sequences obtained from common vole (M. arvalis) captured in the Tula region in Central Russia
[ENHANCEMENT OF THE IMMUNE RESPONSE BY CO-DELIVERY OF THE HEPATITIS C VIRUS RECOMBINANT DNA AND PROTEINS OF REPLI- CATIVE COMPLEX GENETIC VARIANTS OF THE NOROVIRUS GENOTYPE GII.6].
Epifanova NV
Mol Gen Mikrobiol Virusol · 2015 · PMID 27192819
BACKGROUND: Noroviruses--etiological agents of acute enteric infections, mainly related to the genotype GII.4. However, other genotypes of the noroviruses also play an important role in some epidemic seasons or in partic...
BACKGROUND: Noroviruses--etiological agents of acute enteric infections, mainly related to the genotype GII.4. However, other genotypes of the noroviruses also play an important role in some epidemic seasons or in particular geographic regions. The norovirus genotype GII.6 has become the second most important etiologic agent of outbreaks of the norovirus infection after GII.4 in recent years. OBJECTIVE: To characterize the norovirus genotype GII.6 genetic variants based on phylogenetic analysis of genome sequences submitted to the databases GenBank and NoroNet as well as identified in Nizhny Novgorod. MATERIALS AND METHODS: Norovirus genotype GII.6 circulating with sporadic morbidity that had caused the outbreak of acute enteric infection in Nizhny Novgorod were identified by sequencing the region of the genome encoding the N/S-domain of capsid protein VP1. The comparative phylogenetic analysis of obtained sequences and sequences available in the international genetic databases was performed using the MEGA 5.2 software package. RESULTS: The presence of three genetic variants of the noroviruses GII.6 genotype based on capsid protein gene, GII.6a (Seacroft_1990), GII.6b (Saitama_1997) and GII.6c (Shizuoka 2008), in combination with two genotypes of the polymerase gene, P6 and P7, was confirmed. It was shown that co-circulation of these variants from the 1970s reflected the differences in evolution between the minor genotypes of noroviruses and dominant genotype GII.4, whose new epidemic variants completely replaced the previous for several years. Noroviruses GII.6 circulating in Nizhny Novgorod and other cities of Russia belong to genovariants GII.6a and GII.6b. CONCLUSION: The recombinant noroviruses GII.P7_GII.6c became most widespread in Asia and Europe in recent years. Genetic variant GII.6c of the norovirus have not been identified in Russia, but we cannot exclude the possibility of their occurrence as a cause of the outbreaks of acute enteric infections in this country in the near future.
[The Experience of the Identifying Rubella Infection Markers during Local Outbreaks in Western Siberia].
Petrova ID, Petrov VS, Seregin SV … +1 more , Malkova EM
Mol Gen Mikrobiol Virusol · 2015 · PMID 27192818
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[A METHOD FOR DIFFERENTIATION OF BACILLUS ANTHRACIS STRAINS AND PHYLOGENETICALLY RELATED SPECIES BASED ON DETERMINATION OF THE STRUCTURAL DIFFERENCESBETWEEN CHROMOSOMAL GENES FOR BIOSYNTHESIS OF FLAGELLIN AND METHIONINE].
Mikshis NI, Kashtanova TN, Kutyrev VV
Mol Gen Mikrobiol Virusol · 2015 · PMID 27192817
Nucleotide sequence analysis of several genes responsible for the anthrax pathogen definitive properties--motility and penicillinase activity--determined a chromosomal locus promising for interspecies differentiation. We...
Nucleotide sequence analysis of several genes responsible for the anthrax pathogen definitive properties--motility and penicillinase activity--determined a chromosomal locus promising for interspecies differentiation. We demonstrated that the gene fliC encoding flagellin synthesis contains extended region, distinguishing B. anthracis strains from the majority of non-pathogenic and opportunistic bacilli. A novel method for the anthrax pathogen indication and identification based on determination of the differences in the chromosomal genes fliC and hom2 structure was suggested. A total of 60 strains of different Bacillus spp. (B. anthracis, B. cereus, B. thuringiensis, B. mycoides, B. megaterium, B. subtilis, etc.) were tested using two chromosomal DNA targets. The algorithm developed in this work permits to detect the pathogenic microorganism and reliably differentiate it from other Bacillus spp. representatives. The introduction of primers complementary to specific sequences of pXO1 and pXQ2 plasmids into the multiplex PCR makes it possible to receive additional information on proposed virulence of the isolate.
[CITRULLINUREIDASE GENE DIVERSITY IN THE GENUS FRANCISELLA].
Timofeev VS, Bakhteeva IV, Pavlov VM … +1 more , Mokrievich AN
Mol Gen Mikrobiol Virusol · 2015 · PMID 27192816
This work describes the results, of the in silico analysis of the genetic diversity of the citrullinureidase gene (ctu) in two species of bacteria of the genus Francisella: tularensis (ssp. tularensis, holarctica, medias...
This work describes the results, of the in silico analysis of the genetic diversity of the citrullinureidase gene (ctu) in two species of bacteria of the genus Francisella: tularensis (ssp. tularensis, holarctica, mediasiatica, novicida) and philomiragia. The strains of the Central Asiatic subspecies possessing the citrullinureidase activity differ in the gene ctu from the ssp tularensis Schu by three nucleotide substitutions leading to two insignificant amino acid substitutions in the encoded polypeptide. In the strain F. tularensis of the ssp. holarctica the gene ctu encodes inactive enzyme, which is probably due to amino acid substitutions: 151 Gly --> Asp, 183 Pro --> Leu, 222 Asp --> Asn. Except for the Japan biovar bacteria, in all strains of the Holarctic subspecies there are two stop codons in the gene ctu. The bacteria of the subspecies novicida contain the ctu gene only in the strain 3523, whereas the other strains contain the gene FTN_0827 encoding the C-N hydrolase, which probably provides the citrullinureidase activity.
[ASSOCIATION BETWEEN FOUR SEROTONIC GENES POLYMORPHISM (5HTTL, 5HT1A, 5HT2A, AND MAOA) AND PERSONALITY TRAITS IN WRESTLERS AND CONTROL GROUP].
Butovskaya PR, Lazebnij OE, Fekhretdionva DI … +5 more , Vasil'ev VA, Prosikova EA, Lysenko VV, Udina IG, Butovskaya ML
Mol Gen Mikrobiol Virusol · 2015 · PMID 27192815
This study presents the data on the polymorphisms of the serotonin system genes (5-HTTL, 5-HT1A, 5-HT2A, and MAOA) in male and female wrestlers and in the control group. The population genetics analysis of the 5HTTL gene...
This study presents the data on the polymorphisms of the serotonin system genes (5-HTTL, 5-HT1A, 5-HT2A, and MAOA) in male and female wrestlers and in the control group. The population genetics analysis of the 5HTTL gene showed the highest frequency of the SS genotype 5-HTTLPR in sportsmen (p = 0.04), as well as the trend toward higher frequency of united genotypes of the locus of 5-HTTLPR VNTR and SNP rs25531--SASA (p = 0.06) in comparison with the control group. As for the polymorphisms for other genes 5-HT1A (rs6295), 5-HT2A (rs6311), and MAOA (VNTR), we found no significant differences between the groups tested. Using the NEO PI-R questionnaire we analyzed the possible correlations between the genotypes and the psychological traits in our samples. It was demonstrated that the athletic success in elite sportsmen was associated with lower openness to experience and higher conscientiousness. The interaction effect of the gender and 5-HT2A on the self-rating for openness to experience, interaction effect of the level of the sport success and 5-HT2A, and the interaction effect of the gender and 5-HT1A genotype on self-reported conscientiousness were observed as a trend.
[ADULT STEM CELLS AND CELLS OF MALIGNANT ORIGIN. PART II].
Sverdlov ED, Pleshkan VV, Alekseenko IV … +3 more , Vinogradova TV, Kopantzev EP, Didich DA
Mol Gen Mikrobiol Virusol · 2015 · PMID 27192814
Recent data on adult stem cells are reviewed. According to the present dominant paradigm, it is most probable that cancer predisposition arises or cancer is initiated in these cells.
Recent data on adult stem cells are reviewed. According to the present dominant paradigm, it is most probable that cancer predisposition arises or cancer is initiated in these cells.
[Contribution of neuraminidase of influenza viruses to the sensitivity to the serum inhibitors and reassortment efficiency].
Kiseleva IV, Larionova NV, Bazhenova EA … +4 more , Fedeorova EA, Dubrovina IA, Isakova-Sivak IN, Rudenko LG
Mol Gen Mikrobiol Virusol · 2014 · PMID 25335411
The live attenuated influenza vaccine (LAIV) consists of reassortant viruses with hemagglutinin (HA) and neuraminidase (NA) gene segments inherited from the circulating wild-type (WT) parental and the 6 internal protein-...
The live attenuated influenza vaccine (LAIV) consists of reassortant viruses with hemagglutinin (HA) and neuraminidase (NA) gene segments inherited from the circulating wild-type (WT) parental and the 6 internal protein-encoding gene segments from the cold-adapted attenuated master donor viruses (genome composition 6:2). In this study, we describe the obstacles to developing LAIV vaccine strains depending on the phenotypic peculiarities of the WT viruses used for reassortment. The genomic composition analysis of 849 reassortants revealed that over 80% of the reassortants based on the inhibitor-resistant WT viruses inherited WT NA as compared to 26% of reassortants based on the inhibitor-sensitive WT viruses. In addition, the highest percentage of the vaccine genotype reassortants was achieved when WT parental viruses were resistant to the non-specific serum inhibitors. We demonstrate that NA may play a role in the influenza virus sensitivity to the non-specific serum inhibitors. Replacing NA of the inhibitor-sensitive WT virus with the NA of the inhibitor-resistant master donor virus significantly decreased the sensitivity of the resulting reassortant virus to the non-specific inhibitors.
[Search for RNA of the hepatitis E virus autochthonous for Russia in the most likely infection sources].
Voronina OL, Kunda MS, Gudov VP … +6 more , Aksenova EI, Shilova VS, Yarosh LV, Elgort DA, Lunin VG, Semenenko TA
Mol Gen Mikrobiol Virusol · 2014 · PMID 25335410
The research carried out for 30 years from the moment of hepatitis E virus (HEV) discovery has proved the presence of the autochthonous HEV in non-endemic areas: Europe and Russia. Monitoring of the HEV antibodies (anti-...
The research carried out for 30 years from the moment of hepatitis E virus (HEV) discovery has proved the presence of the autochthonous HEV in non-endemic areas: Europe and Russia. Monitoring of the HEV antibodies (anti-HEV) among the Russian population has revealed regions with increased seroprevalence that testifies to high probability of local HEV infection in these areas. Contact with HEV can represent special danger for patients of the risk groups. In this work, the blood sera testing was carried out in order to assess the anti-HEV presence among these contingents (groups). Seropositive sera from the patients from the regions with high anti-HEV seroprevalence, risk groups patients, samples with high probability of HEV occurrence including the animals as possible reservoir, have been used for RNA extraction. The developed system of HEV RNA detection both in real-time RT-PCR and in a nested PCR variant has confirmed its sensitivity to the synthetic reference templates and positive control samples in commercial test system (Genesig, Great Britain). HEV RNA was absent in all tested samples. This indicates a low frequency of the autochthonous HEV carriage occurrence. Sampling enlargement to tens of thousands persons is necessary for significant HEV RNA detection.
[MALDI-TOF mass-spectrometric analysis in the accelerated identification of the Vibrio genus microorganisms].
Afanasev MV, Mironova LV, Basov EA … +4 more , Ostyak AS, Kulikalova ES, Urbanovich LY, Balahonov SV
Mol Gen Mikrobiol Virusol · 2014 · PMID 25335409
The goal of this work was to develop methodological approaches to identification of the Vibrio genus representatives using the MALDI-TOF mass-spectrometric analysis technologies. The aspects of the biological safety in s...
The goal of this work was to develop methodological approaches to identification of the Vibrio genus representatives using the MALDI-TOF mass-spectrometric analysis technologies. The aspects of the biological safety in sample preparations for mass-spectrometric analysis were studied, reference spectra of six typical V. cholerae strains were developed. Identification of 55 strains, representatives of the Vibrio genus, including 45 V. cholerae strains with different epidemic importance, was performed using the MALDI Biotyper 3.0 basis comprising V. cholerae reference spectra. The possibility of reliable definition of the tested strain taxonomic belonging to the species level was demonstrated. Thus, the results completely corresponded to the data of classical microbiological identification. Stability and reproducibility of the offered research method was experimentally shown. The results allow identification of the Vibrio genus representatives to be implemented with the use of the mass-spectrometric analysis as an effective method that defines a species belonging of the basic Vibrio genus representatives in the shortest-terms.
[Polymorphism of the biotransformation gene--cytochrome-450 2C9 in the patients with tuberculosis].
Antonenko PB, Kresyun VI
Mol Gen Mikrobiol Virusol · 2014 · PMID 25335408
The goal of this work was to study cytochrome-450 (CYP) 2C9 (CYP2C9) gene polymorphism in patients with tuberculosis (TB) and its meaning for development, progress, and outcome of TB, for the pharmacokinetics of the anti...
The goal of this work was to study cytochrome-450 (CYP) 2C9 (CYP2C9) gene polymorphism in patients with tuberculosis (TB) and its meaning for development, progress, and outcome of TB, for the pharmacokinetics of the antituberculosis antibiotic rifampicin on the basis of the southern region of Ukraine. Among the TB patients it was 24.9% less than in carriers of the genotype *1/*1 and than in healthy donors. At the same time, it was 25.0% less than in carriers of the genotypes *1/*2, *1/*3. In the TB patientswith the genotype *2/*3, *3/*3 the level of rifampicin in blood was the lowest. At the beginning of the treatment in carriers of genotype *1/*1 the pulmonary destruction was observed 2.5 times more often than in *1/*2, *1/*3 genotype. According to the cultural method, the carriers of *1/*1 more frequently became smear-negative than the carriers of *1/*2, *1/*3 genotype.
[Analysis of fragments of intergenome spacers of human body observed in chromosomes containing no nuclear organization].
Kupriyanova NS, Nechvolodov KK, Korsunenko AV
Mol Gen Mikrobiol Virusol · 2014 · PMID 25335407
Tandem repetitions of rDNA provide so-called nuclear organizations (NOR). On the other hand, rDNA-structures are observed in some NOR chromosomes. It was demonstrated that, in addition to ribosome biogenesis, nucleoli pr...
Tandem repetitions of rDNA provide so-called nuclear organizations (NOR). On the other hand, rDNA-structures are observed in some NOR chromosomes. It was demonstrated that, in addition to ribosome biogenesis, nucleoli provided a number of functions: cell cycle regulation, stress-induced response, transcription regulation, which often induced cell cascades. The mechanisms of the induction of rDNA segments in NOR chromosomes are obscure and require further research. About 1/3 repetitions are associated with nucleoli and SINE/Alu repetitions, homogeneous repetition, and tandem repetition. Perhaps, relative position of nucleoli and chromosomes may facilitate/prevent interaction of chromosomes with rDNA clusters. The variability of two larger repetitions in the central part of rMGS, LR1, and LR2 similar by -90% and separated by several hundred pairs of bases from each other was studied in our previous works. This work was devoted to the search for the LR1-LR2 segments in other chromosomes, characterization of their terminal tips at rupture points and genome areas of incorporation of the LR1-LR2 segments.
[Double-component bacterial regulation systems as a target for searching new antimicrobial agents].
Tiganova IG, Il'ina TS, Romanova YM
Mol Gen Mikrobiol Virusol · 2014 · PMID 25335406
A review of the literature data concerning double-component bacterial regulation systems was given. The observed data concern: a) structural and functional organization based on the example of systems of the family OmpR...
A review of the literature data concerning double-component bacterial regulation systems was given. The observed data concern: a) structural and functional organization based on the example of systems of the family OmpR (EnvZ/OmpR, PhoQ/PhoP), which regulate a number of processes providing adaptation to stress conditions in the environment and host body providing the virulence and biofilm formation, the cause of different human chronic infections; b) the genes and functions regulated by the double-components systems, based on the example of EnvZ/OmpR system, especially OmpR protein, and PhoQ/PhoP system. The possibilities for the searching of the double-component system protein inhibitors and their role in depressing pathogenic bacteria virulence were discussed.
[Comparative multilocus VNTR- and SNP-analysis of Bacillus anthracis vaccine strains].
Afanas'ev MV, Kravets EV, Dugarzhapova ZF … +3 more , Takaĭshvili VE, Polovinkina VS, Balakhonov SV
Mol Gen Mikrobiol Virusol · 2014 · PMID 25080817
Comparative VNTR- and SNP-genotype analysis of four Bacillus anthracis strains (three vaccinal and one virulent) was carried out using modern molecular-genetic typing methods. It is established that these strains formed...
Comparative VNTR- and SNP-genotype analysis of four Bacillus anthracis strains (three vaccinal and one virulent) was carried out using modern molecular-genetic typing methods. It is established that these strains formed four SNP patterns completely corresponding to the VNTR-profiles. It was demonstrated that all strains tested except vaccinal B. anthracis STI-1 could not belong by SNP-profile to three global genetic lines of the anthrax agent extended in the world.
[Retrospective macrorestrictive analysis of the Vibrio cholerae eltor strains isolated at epidemic complications in the Far East of Russia].
Mol Gen Mikrobiol Virusol · 2014 · PMID 25080816
The retrospective study of the clonal structure of 24 Vibrio cholerae eltor strains isolated at epidemic complications in the Far East of Russia was carried out on the basis of the macrorestrictive analysis of the genome...
The retrospective study of the clonal structure of 24 Vibrio cholerae eltor strains isolated at epidemic complications in the Far East of Russia was carried out on the basis of the macrorestrictive analysis of the genome DNA using NotI and SfiI endonucleases. It was found that clonal (by amplification profiling data) toxigenic strains (n = 23) were characterized by variability of NotI/SfiI-generated restriction profiles. The V. cholerae eltor isolated in Yuzhno-Sakhalinsk outbreak were differentiated to four pulse-types with one dominating type detected in 76% of the strains. Individual restrictive NotI/SfiI-profile was characteristic for the isolates from Primorye Territory. A non-toxigenic strain formed a separate dendrogram line from the toxigenic strains on the basis of PFGE-pattern structure. Efficiency of macrorestriction analysis application for the profound characteristic of the V. cholerae population structure and ascertainment of molecular-epidemiological regularities of territorial distribution of the separate V. cholerae clones was shown.
[Construction of recombinant adenoviral vector expressing genes of the conservative influenza proteins M2 and nucleoprotein].
Esmagambetov IB, Sedova ES, Shcherbinin DN … +4 more , Lysenko AA, Garas MN, Shmarov MM, Logunov DIu
Mol Gen Mikrobiol Virusol · 2014 · PMID 25080815
Influenza is a highly contagious and one of the most massive infection diseases. General epidemiological significance has a strain, which belongs to subtype A. A high degree of genetic variety leads to the permanent chan...
Influenza is a highly contagious and one of the most massive infection diseases. General epidemiological significance has a strain, which belongs to subtype A. A high degree of genetic variety leads to the permanent changes in the antigenic structure of the influenza virus. Therefore, the current influenza vaccines require periodic updating of the composition of strains. Presently, it is important to develop a universal vaccine that can protect against different strains of influenza A virus at the same time and is based on the conserved antigens of the influenza virus. The recombinant adenovirus vectors expressing genes of conserved viral antigenes may be a promising candidate vaccine against influenza A. Using the method of the homologous recombination, we developed in this study recombinant adenovirus of fifth serotype that expresses genes of the ion channel M2 and nucleoprotein NP of the influenza virus A. Genes of the consensus protein M2 and NP of human influenza A virus were included into the structure of the viral genome. The expression of the antigens M2 and NP using recombinant adenovirus vector was detected by a Western blot assay. The immunogenicity of the developed recombinant adenovirus vector was demonstrated by the intranasal immunization of laboratory mice.
[Regulation of Yersinia pseudotuberculosis major porin expression in response to antibiotic stress].
Bystritskaia EP, Stenkova AM, Portniagina OIu … +3 more , Rakin AV, Rasskazov VA, Isaeva MP
Mol Gen Mikrobiol Virusol · 2014 · PMID 25080814
The OmpF porin gene expression in Yersinia pseudotuberculosis in response to antibiotics of two different classes (kanamycin and nalidixic acid) was analyzed using quantitative PCR and a fluorescence reporter system. Bot...
The OmpF porin gene expression in Yersinia pseudotuberculosis in response to antibiotics of two different classes (kanamycin and nalidixic acid) was analyzed using quantitative PCR and a fluorescence reporter system. Both antibiotics downregulated the expression of the ompF gene. The nalidixic acid significantly reduced ompF expression, while kanamycin, for which porins are considered to be an alternative transport route, only slightly reduced the ompF level.
[Identification of ubiquitous and endemic Mycobacterium tuberculosis genotypes in the Republic of Buryatia].
Zhdanova SN, Ogarkov OB, Lats AA … +4 more , Zarbuev AN, Badleeva MB, Untanova LS, Savilov ED
Mol Gen Mikrobiol Virusol · 2014 · PMID 25080813
The MIRU-12 and MIRU-24 methods were used for genotyping and estimation of M. tuberculosis population structure in Buryatia. It was studied in 283 strains isolated from individuals from the regional TB dispensary and med...
The MIRU-12 and MIRU-24 methods were used for genotyping and estimation of M. tuberculosis population structure in Buryatia. It was studied in 283 strains isolated from individuals from the regional TB dispensary and medical clinics of the penitentiary system of the republic. It was found that the prevalence of pandemic genotypes Beijing MIT17 and MIT16 and also subtype Beijing MIT642 (24.5% - 46/188) in the studied area were dominant. The isolates of the Beijing MIT642 were common among all groups of patients with pulmonary tuberculosis with the tendency to formation of MDR and chronic course of the disease.
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