Biotechnology, Biotechnological Equipment[JOURNAL]
Sun
200 papers
Conversion of yellow wine lees into high-protein yeast culture by solid-state fermentation.
Hu Y, Pan L, Dun Y … +3 more , Peng N, Liang Y, Zhao S
Biotechnol Biotechnol Equip · 2014 Sep · PMID 26019568 · Full text
This study is focussed on the possibility of producing a yeast culture with yellow wine lees as a substrate by solid-state fermentation (SSF). Results showed that a yeast count of 1.58 × 10 CFU/g was achieved by signal f...
This study is focussed on the possibility of producing a yeast culture with yellow wine lees as a substrate by solid-state fermentation (SSF). Results showed that a yeast count of 1.58 × 10 CFU/g was achieved by signal factor and orthogonal experiments. After fermentation, the starch content in the yeast culture reduced from 32.2% ± 0.5% to 7.5% ± 0.2%, and the contents of crude protein and peptide increased from 36.1% ± 0.8% to 48.0% ± 1.0% and 3.9% ± 0.2% to 7.2% ± 0.4%, respectively. Additionally, large amounts of short peptides and free amino acids were detected by fast protein liquid chromatography (FPLC). These results suggest that yellow wine lees are a suitable substrate for the production of yeast cultures. It can serve as a growth-promoting factor and help reduce the shortage of protein feed in the animal industry. This research provides a potential way for the utilization of agro-industrial residues.
Immobilization of sp. DG17 onto sodium alginate-attapulgite-calcium carbonate.
Wang HQ, Hua F, Zhao YC … +2 more , Li Y, Wang X
Biotechnol Biotechnol Equip · 2014 Sep · PMID 26019567 · Full text
A strain of sp. DG17, capable of degrading crude oil, was immobilized in sodium alginate-attapulgite-calcium carbonate for biodegradation of crude oil contaminated soil. In this work, proportion of independent variables...
A strain of sp. DG17, capable of degrading crude oil, was immobilized in sodium alginate-attapulgite-calcium carbonate for biodegradation of crude oil contaminated soil. In this work, proportion of independent variables, the laboratory immobilization parameters, the micromorphology and internal structure of the immobilized granule, as well as the crude oil biodegradation by sodium alginate-attapulgite-calcium carbonate immobilized cells and sodium alginate-attapulgite immobilized cells were studied to build the optimal immobilization carrier and granule-forming method. The results showed that the optimal concentrations of sodium alginate-attapulgite-calcium carbonate and calcium chloride were 2.5%-3.5%, 0.5%-1%, 3%-7% and 2%-4%, respectively. Meanwhile, the optimal bath temperature, embedding cell amount, reaction time and multiplication time were 50-60 °C, 2%, 18 h and 48 h, respectively. Moreover, biodegradation was enhanced by immobilized cells with a total petroleum hydrocarbon removal ranging from 33.56% ± 3.84% to 56.82% ± 3.26% after 20 days. The SEM results indicated that adding calcium carbonate was helpful to form internal honeycomb-like pores in the immobilized granules.
Genetic characterization of MHC class II DQB exon 2 variants in gayal ().
Sun Y, Xi D, Li G … +3 more , Hao T, Chen Y, Yang Y
Biotechnol Biotechnol Equip · 2014 Sep · PMID 26019566 · Full text
In the present study, exon 2 of major histocompatibility complex (MHC) class II DQB gene from 39 gayals () was isolated, characterized and compared with previously reported patterns for other bovidae. It was revealed by...
In the present study, exon 2 of major histocompatibility complex (MHC) class II DQB gene from 39 gayals () was isolated, characterized and compared with previously reported patterns for other bovidae. It was revealed by sequence analyses that there are 36 DQB exon 2 variants among 39 gayals. These variants exhibited a high degree of nucleotide and amino acid substitutions with most amino acid variations occurring at positions forming the peptide-binding sites (PBS). The DQB loci were analysed for patterns of synonymous () and non-synonymous () substitution. The gayals were observed to be under strong balancing selection in the DQB exon 2 PBS ( = 0.094, = 0.001). It appears that this variability among gayals could confer the ability to mount immune responses to a wide variety of peptides or pathogens.
Characterization of two acetyltransferase genes in the pyripyropene biosynthetic gene cluster from .
Hu J, Furutani A, Yamamoto K … +3 more , Oyama K, Mitomi M, Anzai H
Biotechnol Biotechnol Equip · 2014 Sep · PMID 26019565 · Full text
Pyripyropenes potently and selectively inhibit acyl-CoA:cholesterol acyltransferase 2 (ACAT-2). Among multiple isomers of pyripyropene (A to R), pyripyropene A (PyA) has insecticidal properties in addition to its growth...
Pyripyropenes potently and selectively inhibit acyl-CoA:cholesterol acyltransferase 2 (ACAT-2). Among multiple isomers of pyripyropene (A to R), pyripyropene A (PyA) has insecticidal properties in addition to its growth inhibition properties against human umbilical vein endothelial cells. Based on the predicted biosynthetic gene cluster of pyripyropene A, two genes ( and ) encoding two acetyltransferases (ATs) were separately isolated and introduced into the model fungus , using the protoplast-polyethylene glycol method. The bioconversion of certain predicted intermediates in the transformants revealed the manner by which acetylation occurred in the biosynthetic pathway by the products expressed by these two genes (AT-1 and AT-2). The acetylated products detected by high-performance liquid chromatography (HPLC) in the extracts from AT-1 and AT-2 transformant clones were not present in the extract from the transformant clone with an empty vector. The HLPC charts of each bioconversion study exhibited high peaks at 12, 10.5 and 9 min, respectively. Further ultraviolet absorption and mass spectrometry analyses identified the products as PyE, PyO and PyA, respectively. AT-1 acetylated the C-1 of deacetyl-pyripyropene E (deAc-PyE), while AT-2 played an active role in acetylating the C-11 of 11-deAc-PyO and C-7 of deAc-PyA at two different steps of the biosynthetic pathway.
Molecular characterization of three common olive ( L.) cultivars in Palestine, using simple sequence repeat (SSR) markers.
Obaid R, Abu-Qaoud H, Arafeh R
Biotechnol Biotechnol Equip · 2014 Sep · PMID 26019564 · Full text
Eight accessions of olive trees from three common varieties in Palestine, Nabali Baladi, Nabali Mohassan and Surri, were genetically evaluated using five simple sequence repeat (SSR) markers. A total of 17 alleles from 5...
Eight accessions of olive trees from three common varieties in Palestine, Nabali Baladi, Nabali Mohassan and Surri, were genetically evaluated using five simple sequence repeat (SSR) markers. A total of 17 alleles from 5 loci were observed in which 15 (88.2%) were polymorphic and 2 (11.8%) were monomorphic. An average of 3.4 alleles per locus was found ranging from 2.0 alleles with the primers GAPU-103 and DCA-9 to 5.0 alleles with U9932 and DCA-16. The smallest amplicon size observed was 50 bp with the primer DCA-16, whereas the largest one (450 bp) with the primer U9932. Cluster analysis with the unweighted pair group method with arithmetic average (UPGMA) showed three clusters: a cluster with four accessions from the 'Nabali Baladi' cultivar, another cluster with three accessions that represents the 'Nabali Mohassen' cultivar and finally the 'Surri' cultivar. The similarity coefficient for the eight olive tree samples ranged from a maximum of 100% between two accessions from Nabali Baladi and also in two other samples from Nabali Mohassan, to a minimum similarity coefficient (0.315) between the Surri and two Nabali Baladi accessions. The results in this investigation clearly highlight the genetic dissimilarity between the three main olive cultivars that have been misidentified and mixed up in the past, based on conventional morphological characters.
Development of a core collection for ramie by heuristic search based on SSR markers.
Luan MB, Zou ZZ, Zhu JJ … +5 more , Wang XF, Xu Y, Ma QH, Sun ZM, Chen JH
Biotechnol Biotechnol Equip · 2014 Sep · PMID 26019563 · Full text
There are more than 2000 ramie germplasms in the National Ramie Germplasm Nursery affiliated with the Institute of Bast Fiber Crops, Chinese Academy of Agricultural Science, China. As it is difficult to perform effective...
There are more than 2000 ramie germplasms in the National Ramie Germplasm Nursery affiliated with the Institute of Bast Fiber Crops, Chinese Academy of Agricultural Science, China. As it is difficult to perform effective conservation, management, evaluation, and utilization of redundant genetic resources, it is necessary to construct a core collection by using molecular markers. In this study, a core collection of ramie consisting of 22 germplasms was constructed from 108 accessions by heuristic search based on 21 Simple Sequence Repeat (SSR) marker combinations. The results showed that there is a poor relationship between the core collection and the geographic distribution. The number of amplification bands for the core collection was the same as that for the entire collection. Shannon's index for three of the SSR primers (14%) and Nei's index for nine of the SSR primers (19%) were lower in the core collection than in the entire collection. The true core collection had wider genetic diversity compared with the random core collection. Collectively, the core collection constructed in this study is reliable and represents the genetic diversity of all the 108 accessions.
Osmoregulation capacity in Bulgarian durum wheat.
Todorovska EG, Bozhanova V, Dechev D … +1 more , Valkova N
Biotechnol Biotechnol Equip · 2014 Sep · PMID 26019562 · Full text
The phenotypic variation in osmotic adjustment (OA) capacity of five Bulgarian winter durum wheat genotypes and their progenies was determined using a modified method based on the measurement of seedling growth suppressi...
The phenotypic variation in osmotic adjustment (OA) capacity of five Bulgarian winter durum wheat genotypes and their progenies was determined using a modified method based on the measurement of seedling growth suppression after three-day exposure to osmotic stress induced by 1 mol/L sucrose. The genetic parameters of the studied trait in a diallel crossing scheme, including the selected genotypes and the microsatellite polymorphism at 43 loci, were determined. The old Bulgarian cultivar Apulicum 233 and all hybrid combinations involving this genotype showed higher OA. In the heritability of osmoregulation ability, the non-additive gene effects (specific combining ability) strongly predominated over the additive ones and had a significant impact on the observed high heterosis effect. Distinct polymorphisms were identified between the studied genotypes. Cluster analysis of the phenotypic data obtained from a multiyear test under water-limited conditions and the molecular data, both based on Euclidean distance, showed similar grouping of the genotypes with specific separation of cultivar Apulicum 233 (high OA) in a single cluster. Principal component analysis revealed not only interrelationships between the important agronomic and morpho-physiological traits in Bulgarian durum wheat under water-limited conditions, but also presence of relations between them and some microsatellite loci located near or within known quantitative trait loci (QTLs) for these traits. Further studies based on segregating population between genotypes with contrasting levels of OA will allow mapping QTLs for phenotypic traits expressed under water deficit and isolation of genes that can be used as potential markers in marker-assisted selection for drought tolerance.
Comparison of health-related quality-of-life measurement instruments in diabetic patients.
Yordanova S, Petkova V, Petrova G … +4 more , Dimitrov M, Naseva E, Dimitrova M, Petkova E
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019561 · Full text
The objective of the study was to compare three different questionnaires (Short Form (SF)-6D, EuroQuol (EQ)-5D and WHO-5) to establish which one is more sensitive and which one gives an adequate assessment of the quality...
The objective of the study was to compare three different questionnaires (Short Form (SF)-6D, EuroQuol (EQ)-5D and WHO-5) to establish which one is more sensitive and which one gives an adequate assessment of the quality of life in patients with diabetes. In an observational and transversal study with duration of 4 months, in 5 Bulgarian cities, 146 patients were randomly selected. The following quality-of-life measuring instruments were applied: 146 questionnaires SF-6D, 146 questionnaires EQ-5D and 103 questionnaires of WHO-5. Descriptive statistics, chi-Square and correlation coefficients were used for data analysis. The study assessed the quality of life of patients suffering from diabetes mellitus with a mean age of 57.39 years (standard deviation (SD) 17.087); 95% confidence interval (CI) 54.60-60.19; 76% of the patients had diabetes type 2. The patients received a mean SF-6D score of 0.6290, an EQ-5D score of 0.6272, a visual analogue scale score of 0.7158 and a WHO-5 score of 0.4635. Preferences measured by the SF-6D and by the EQ-5D showed significant correlations with one another, and the Pearson coefficient was = 0.906 ( < 0.01). The most current version of SF-6D, based on the 2002 model, was found to be valid and reliable when compared to the EQ-5D and is a questionnaire alternative to assess preferences in economic analysis carried out in health care.
Insights into 3-hydroxypropionic acid biosynthesis revealed by overexpressing native glycerol dehydrogenase in .
Su MY, Li Y, Ge XZ … +1 more , Tian PF
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019560 · Full text
In , glycerol dissimilation involves parallel oxidation and reduction pathways. Oxidation pathway provides adenosine triphosphate (ATP) and cofactors to sustain cell growth, while reduction pathway presents 3-hydroxyprop...
In , glycerol dissimilation involves parallel oxidation and reduction pathways. Oxidation pathway provides adenosine triphosphate (ATP) and cofactors to sustain cell growth, while reduction pathway presents 3-hydroxypropionic acid (3-HP) and 1,3-propanediol(1,3-PDO), which are commercially attractive platform chemicals. Previous metabolic engineering of . focused on the intensification of reduction pathway; however, it failed to overproduce 3-HP or 1,3-PDO. Contrary to this strategy, here we show that overexpression of glycerol dehydrogenase (dhaD), the first functional enzyme in oxidation pathway, can efficiently stimulate cell growth and facilitate 3-HP accumulation. Under microaerobic conditions, although metabolic burden arising from plasmid replication, the recombinant overexpressing grew actively and showed 60% enhancement of 3-HP compared to the control. In particular, overexpression of dhaD increased the activity of glycerol dehydratase, indicating the concerted action of two enzymes and the interdependence between glycerol oxidation and reduction pathways. Moreover, the strain overexpressing dhaD produced more lactic acid yet less acetic acid than the control, implying the interplay between dhaD expression and the formation of byproducts. Together, not only showing that intensifying glycerol oxidation pathway is beneficial to 3-HP production, this study also reveals the structural rigidity of operon that mediates glycerol dissimilation in . .
GECluster: a novel protein complex prediction method.
Su L, Liu G, Wang H … +4 more , Tian Y, Zhou Z, Han L, Yan L
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019559 · Full text
Identification of protein complexes is of great importance in the understanding of cellular organization and functions. Traditional computational protein complex prediction methods mainly rely on the topology of protein-...
Identification of protein complexes is of great importance in the understanding of cellular organization and functions. Traditional computational protein complex prediction methods mainly rely on the topology of protein-protein interaction (PPI) networks but seldom take biological information of proteins (such as Gene Ontology (GO)) into consideration. Meanwhile, the environment relevant analysis of protein complex evolution has been poorly studied, partly due to the lack of high-precision protein complex datasets. In this paper, a combined PPI network is introduced to predict protein complexes which integrate both GO and expression value of relevant protein-coding genes. A novel protein complex prediction method GECluster (Gene Expression Cluster) was proposed based on a seed node expansion strategy, in which a combined PPI network was utilized. GECluster was applied to a training combined PPI network and it predicted more credible complexes than peer methods. The results indicate that using a combined PPI network can efficiently improve protein complex prediction accuracy. In order to study protein complex evolution within cells due to changes in the living environment surrounding cells, GECluster was applied to seven combined PPI networks constructed using the data of a test set including yeast response to stress throughout a wine fermentation process. Our results showed that with the rise of alcohol concentration, protein complexes within yeast cells gradually evolve from one state to another. Besides this, the number of core and attachment proteins within a protein complex both changed significantly.
Evaluation of different pretreatments on microbial transformation of saponins in for diosgenin production.
Zheng T, Yu L, Zhu Y … +1 more , Zhao B
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019558 · Full text
In order to evaluate the effects of different pretreatments on microbial transformation of saponins in (DZW), various methods have been systematically studied on a large scale. Five pretreatments, including physical sep...
In order to evaluate the effects of different pretreatments on microbial transformation of saponins in (DZW), various methods have been systematically studied on a large scale. Five pretreatments, including physical separation, catalytic solvent extraction, ultrasonic fermentation, complex enzymatic hydrolyzation and enzymatic saccharification, were performed on DZW. Compared with other methods, complex enzymatic hydrolyzation significantly improved the efficiency of microbial transformation. Due to the pretreatment, a diosgenin yield of 92.6%, and diosgenin accumulation of 27.3 mg/g DZW were achieved. The high efficiency of this method was attributed to the separation of 84.3% starch and 76.5% fibre from DZW in the form of a sugar. Analysis of saponins in this microbial transformation process showed that the residual rates of the intermediate products were much lower than those obtained from other pretreatments. The results demonstrate that complex enzymatic hydrolyzation is a practical and effective pretreatment method for production of diosgenin from DZW in a microbial transformation way.
Comparative study of cytotoxicity of detonation nanodiamond particles with an osteosarcoma cell line and primary mesenchymal stem cells.
Keremidarska M, Ganeva A, Mitev D … +4 more , Hikov T, Presker R, Pramatarova L, Krasteva N
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019557 · Full text
Recently, nanodiamonds (NDs) have attracted great interest due to their unique physical and chemical properties that could be used in various biological applications. However, depending on the origin, NDs often contain d...
Recently, nanodiamonds (NDs) have attracted great interest due to their unique physical and chemical properties that could be used in various biological applications. However, depending on the origin, NDs often contain different impurities which may affect cellular functions and viability. Therefore, before their biomedical application, the cytotoxicity of newly produced NDs should be assessed. In the present study, we have evaluated cytotoxicity of four types of ND particles with two cell models: a human osteosarcoma cell line, MG-63, and primary rat mesenchymal stem cells (rMSCs). Detonation-generated nanodiamond (DND) particles were purified with different acid oxidizers and impurities' content was determined by elemental analysis. The particles size distribution was measured revealing that the DND particles have an average size in the range of 51-233 nm. Cytotoxicity was assessed by optical microscopy and proliferation assay after 72 hours exposure of the cells to nanoparticles. We observed cell-specific and material-specific toxicity for all tested particles. Primary stem cells demonstrated higher sensitivity to DND particles than osteosarcoma cells. The most toxic were the DND particles with the smallest grain size and slight content of non-diamond carbon, while DNDs with higher grain size and free from impurities had no significant influence on cell proliferation and morphology. In addition, the smaller DND particles were found to form large aggregates mainly during incubation with rMSCs. These results demonstrate the role of the purification method on the properties of DND particles and their cytotoxicity as well as the importance of cell types used for evaluation of the nanomaterials.
Antibacterial potential of streptomycete strains from Antarctic soils.
Encheva-Malinova M, Stoyanova M, Avramova H … +4 more , Pavlova Y, Gocheva B, Ivanova I, Moncheva P
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019556 · Full text
The exploration of habitats with unusual environment and poorly explored areas such as Antarctica is one of the strategies for discovery of new biologically active substances and/or new producers. The aim of this study w...
The exploration of habitats with unusual environment and poorly explored areas such as Antarctica is one of the strategies for discovery of new biologically active substances and/or new producers. The aim of this study was to identify the actinomycetes isolated from the soils of the island Livingston - Antarctica and to investigate their potential to synthesize antibacterial agents against phytopathogens. Twenty-three actinomycete strains were the object of this study. Using PCR (polymerase chain reaction) amplification all strains were affiliated to genus . The sequencing of the 16S rRNA for three of the strains showed greatest similarity to for one of them, and revealed that the other strains had closest relations to streptomycetes isolated from anthropogenically unaltered regions including Antarctica. The isolates were studied for production of antibacterial substances both by molecular and culture methods. PCR targeting specific biosynthetic genes involved in the production of some groups of antibiotics was performed. The screening showed that all strains possessed the gene for Type-II polyketide synthase, 11 strains - for non-ribosomal peptide synthetase; 6 strains - for polyene antibiotics; and 4 strains - for glycopeptide antibiotics. The production of antibacterial substances by the strains was tested in vitro against phytopathogenic bacteria. The strains differed in the number of inhibited test - bacteria and in their spectrum of action. Four strains showed a wide range of activity against Gram-positive and Gram-negative phytopathogens. The results obtained revealed that the Antarctic soils are potential source for isolation of streptomycetes producing antibiotics from different groups.
Effect of recombinant Hepatitis B virus on human glomerular mesangial cell apoptosis.
Wang Y, Liu C, Hong S … +2 more , Zhang P, Liu Q
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019555 · Full text
The aim of this study was to investigate the expression of recombinant Hepatitis B virus (HBV) in normal human glomerular mesangial cells (NHMC) and its effect on cell apoptosis. Cell transfection was conducted by the li...
The aim of this study was to investigate the expression of recombinant Hepatitis B virus (HBV) in normal human glomerular mesangial cells (NHMC) and its effect on cell apoptosis. Cell transfection was conducted by the liposome method. The levels of HBsAg and HBeAg in the culture supernatant were detected by electrochemiluminescence. Morphological changes were observed by light and fluorescence microscopy. Cell proliferation was analysed by the methyl thiazole tetrazolium (MTT) assay and cell apoptosis, by flow cytometry. The expression level of Bax and Bcl-2 mRNA was measured by semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR). Caspase-3 activity was detected by a Caspase-3 activity detection kit. The results showed high expression levels of HBsAg and HBeAg in NHMC cells transfected with recombinant full-length C genotype HBV (PHY106-CHBV). Typical apoptotic morphology was observed at 48 h after PHY106-CHBV transfection. Cell proliferation was inhibited. The percentage of apoptotic cells and the expression level of Bax mRNA were significantly higher in the PHY106-CHBV group than those in the blank control group and the PHY106 group. There was no significant difference in the expression level of Bcl-2 mRNA among the three groups. Caspase-3 was significantly activated after PHY106-CHBV transfection. The results demonstrate that recombinant HBV can be expressed in NHMC and its expression induces NHMC apoptosis.
Antiviral activity of SPM1605 against Coxsackievirus B3.
Kim MJ, Lee DK, Park JE … +3 more , Park IH, Seo JG, Ha NJ
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019554 · Full text
Bifidobacteria are considered one of the most beneficial probiotics and have been widely studied for their effects against specific pathogens. The present study investigated the antiviral activity of probiotics isolated...
Bifidobacteria are considered one of the most beneficial probiotics and have been widely studied for their effects against specific pathogens. The present study investigated the antiviral activity of probiotics isolated from Koreans against Coxsackievirus B3 (CVB3). The effect of probiotic isolates against CVB3 was measured by the plaque assay and cellular toxicity of bifidobacteria in HeLa cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Among 13 probiotic isolates, 3 , 2 and 1 had an antiviral effect against CVB3, while the others did not show such effect. SPM1605 showed the greatest inhibitory properties against CVB3. When the threshold cycle (CT) values for the treated SPM1605 samples were compared to the results for the non-treated samples, it was shown that the amplified viral sequences from the CVB3 had their copy number lowered by SPM1605. Moreover, the gene expression in infected HeLa cells was also inhibited by 50%. The results suggest that SPM1605 suppresses CVB3 and could be used as an alternative therapy against infectious diseases caused by coxsackieviruses.
Protective effects of ethyl pyruvate in cisplatin-induced nephrotoxicity.
Kelle I, Akkoc H, Tunik S … +3 more , Nergiz Y, Erdinc M, Erdinc L
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019553 · Full text
This study was performed to investigate the effect of ethyl pyruvate on changes in renal functions and oxidative stress related renal injury caused by cisplatin (cis-dichlorodiammine platinum-II; CDDP). Male Wistar albin...
This study was performed to investigate the effect of ethyl pyruvate on changes in renal functions and oxidative stress related renal injury caused by cisplatin (cis-dichlorodiammine platinum-II; CDDP). Male Wistar albino rats were divided into four groups ( = 8): (1) control group (1 ml Ringer's lactate solution i.p.); (2) ethyl pyruvate (EP) group (50 mg/kg Ringer's EP solution (REPS) i.p.); (3) cisplatin group (a single dose of cisplatin (5 mg/kg, i.p.); and (4) cisplatin + EP group (a single dose of cisplatin (5 mg/kg, i.p.) + REPS 50 mg/kg/day, i.p.) for five days. At the sixth day, kidneys of rats were mounted to a Langendorff apparatus. Renal perfusion pressures were recorded. Blood samples were taken for serum urea, creatinine, total oxidant status (TOS), total antioxidant status (TAS) and oxidative stres index (OSI) evaluations. Kidney tissues were obtained for malondialdehyde (MDA) analyses and histopathological examination. Perfusion pressures, serum urea, creatinine, TOS, OSI and tissue MDA levels were found significantly higher, whereas TAS was notably lower in cisplatin group. Histopathological examination showed apparent renal paranchymal injury in cisplatin group. In cisplatin + REPS group, perfusion pressures, serum urea, creatinine and tissue MDA levels were decreased. Moreover, EP co-administration provided less inflammatory cell infiltration, tubular dilatation, whereas TOS, TAS and OSI improved significantly versus cisplatin group. These findings show that EP has protective effects against cisplatin nephrotoxicity.
Heavy metal accumulation in the bark and leaves of planted in Artvin City, Turkey.
Dogan Y, Unver MC, Ugulu I … +2 more , Calis M, Durkan N
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019552 · Full text
The aim of this study was to determine the level of heavy metals such as copper, iron, manganese, zinc, lead, nickel, cadmium and chromium concentrated in bark and leaf samples from different localities in Artvin, Turke...
The aim of this study was to determine the level of heavy metals such as copper, iron, manganese, zinc, lead, nickel, cadmium and chromium concentrated in bark and leaf samples from different localities in Artvin, Turkey. Analysis of the heavy metals Cu, Fe, Mn, Zn, Pb, Ni, Cd and Cr in samples was carried out by inductively coupled plasma optical emission spectroscopy (ICP-OES; Perkin Elmer, Optima 8000 DV). Statistical significance was determined by analysis of variance (ANOVA). The comparisons were performed in order to determine whether there were any differences between bark and leaf samples in terms of average heavy metal accumulation levels. As a result of this study, the following mean concentrations were determined for bark samples: the contents of Cu, Fe, Mn, Zn, Pb, Ni, Cd and Cr (μg g, dry weight) ranged from 72.46 to 88.14, 14.40 to 628.0, 0.896 to 67.71, 7.000 to 28.52, 0.040 to 0.905, 1.031 to 2.744, 0.011 to 0.158 and 1.192 to 3.134, respectively. On the other hand, for leaf samples, the contents of Cu, Fe, Mn, Zn, Pb, Ni, Cd and Cr (μg g, dry weight) ranged from 0.339 to 13.80, 12.72 to 698.2, 1.001 to 204.6, 7.362 to 56.03, 0.158 to 0.665, 0.130 to 2.744, 0.041 to 0.114 and 0.508 to 2.767, respectively. In the statistical analysis, heavy metal accumulation values of bark and leaf samples for Cu, Ni and Cr were significantly different ( < 0.05).
Microbial community development of biofilm in Amaranth decolourization technology analysed by FISH.
Belouhova M, Schneider I, Chakarov S … +2 more , Ivanova I, Topalova Y
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019551 · Full text
The aim of this study was to elucidate the role, the space distribution and the relationships of the bacteria from the genus in a biofilm community during semi-continuous Amaranth decolourization process in model sand b...
The aim of this study was to elucidate the role, the space distribution and the relationships of the bacteria from the genus in a biofilm community during semi-continuous Amaranth decolourization process in model sand biofilters. The examined parameters of the process were as follows: technological parameters; key enzyme activities (azoreductase, succinate dehydrogenase, catechol-1,2-dioxygenase, catechol-2,3-dioxygenase); the number of azo-degrading bacteria and the bacteria from genus (plate count technique); the amount and the location of sp. using fluorescent in situ hybridization (FISH). The results showed that the increase of the Amaranth removal rate with 120% was accompanied with increase of the enzyme activities of the biofilm (azoreductase activity - with 25.90% and succinate dehydrogenase - with 10.61%). The enzyme assays showed absence of activity for сatechol-1,2-dioxygenase and catechol-2,3-dioxygenase at the early phase and high activities of the same oxygenases at the late phase (2.76 and 1.74 μmol/min mg protein, respectively). In the beginning of the process (0-191 h), the number of the culturable microorganisms from genus was increased with 48.76% but at the late phase (191-455 h) they were decreased with 15.25% while the quantity of the non-culturable bacteria from this genus with synergetic relationships was increased with 23.26%. The dominant microbial factors were identified in the structure of the biofilm during the azo-degradation process by using FISH analysis. Furthermore, the inner mechanisms for increase of the rate and the range of the detoxification were revealed during the complex wastewater treatment processes.
Lactic acid microflora of the gut of snail .
Koleva Z, Dedov I, Kizheva J … +3 more , Lipovanska R, Moncheva P, Hristova P
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019550 · Full text
The intestinal lactic acid microflora of the edible snail was studied by culture-based methods and was phenotypically and molecularly characterized. The antibacterial activity of lactic acid bacteria (LAB) isolates was...
The intestinal lactic acid microflora of the edible snail was studied by culture-based methods and was phenotypically and molecularly characterized. The antibacterial activity of lactic acid bacteria (LAB) isolates was investigated. Snails in different stages of development were collected from farms located in several regions of Bulgaria. One hundred twenty-two isolates, belonging to the group of LAB, were characterized morphologically and were divided into four groups. Representative isolates from each morphological type were subjected to phenotypic characterization and molecular identification. The snail gut lactic acid microflora was composed by (17 isolates), (12 isolates), (7 isolates), (18 isolates) and (1 isolate). The species affiliation of (12), (4) and (2) was confirmed by species-specific primers. The isolates were identified by sequence analysis of 16S rDNA as (12), (2), (1) and (3). The species , and were found in snails in a phase of hibernation, whereas was identified both in active and hibernation phases. Antibacterial activity (bacteriocine-like) was shown only by one strain of P4/8 against . The present study showed that the LAB are a component of the microbial communities in the snail digestive system. This is the first report on strains detected in the gut of .
Gene cloning of an important eukaryotic translation initiation factor family, gene in halophytic (Trin.).
Jin CW, Sun YL, Hong SK
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019549 · Full text
Eukaryotic initiation factors eIF2A and eIF2 both play important roles in the mRNA translation of protein synthesis, whereas the functions of eIF2A are usually overlooked, as both functions of binding methyionly-tRNA (Me...
Eukaryotic initiation factors eIF2A and eIF2 both play important roles in the mRNA translation of protein synthesis, whereas the functions of eIF2A are usually overlooked, as both functions of binding methyionly-tRNA (Met-tRNA) to 40S are similar under the same complementary factor and nucleotide requirements. Recently, the functions of eIF2A were reported to differ from those of eIF2 in manners when binding Met-tRNA to 40S. Given that eukaryotic initiation factor eIF2 has been well known, eIF2A was still deficient in understanding of its sequence, structure and functions. In this work, we collected a high salt-tolerant grass (Trin.) as the object of study, and cloned and sequenced the gene from this species. Based on the DNA alignment and analysis of gene sequences from other organisms, an effective primer set was newly designed. Using this primer set, a DNA fragment with length of about 500 bp was obtained, and we have submitted this sequencing result to NCBI GenBank database (accession number: KF279515). The Basic Local Alignment Search Tool (BLAST) result showed that our sequence is highly identical to eIF2A gene sequences that existed in NCBI GenBank database. This work would help to further understand the function of , and provide more potential target genes for studying their functions in relation to stress tolerance mechanisms.
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