Biotechnology, Biotechnological Equipment[JOURNAL]
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200 papers
Changes in protein thiols in response to salt stress in embryogenic suspension cultures of L.
Zagorchev L, Terzieva M, Stoichkova M … +1 more , Odjakova M
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019548 · Full text
The aim of the present study is to assess the rate of protein disulphide formation and the activity of NADPH-dependent thioredoxin and glutaredoxin systems, responsible for the reverse reduction of protein and mixed prot...
The aim of the present study is to assess the rate of protein disulphide formation and the activity of NADPH-dependent thioredoxin and glutaredoxin systems, responsible for the reverse reduction of protein and mixed protein-glutathione disulphides, in embryogenic suspension cultures of , subjected to salt stress. Two concentrations of NaCl previously established as enhancing (0.085 mol/L) and inhibiting (0.17 mol/L) somatic embryogenesis were used. The quantitative (by colour reaction with Ellman's reagent) and qualitative (by diagonal gel electrophoresis) analyses showed a significant increase in protein disulphide formation in salt-treated cultures compared to controls. The ratio of disulphides to free thiols is higher in 0.17 mol/L NaCl-treated cultures. The activity of the thioredoxin-thioredoxin reductase system has been increased accordingly in 0.085 mol/L NaCl-treated cultures but decreased at the higher salt concentration. The activity of glutaredoxins was also estimated, by using glutathionylated bovine serum albumin as substrate and following the decrease of NADPH absorbance at 340 nm in the presence of glutathione and glutathione reductase. Mild salt (0.085 mol/L NaCl) treated cultures again showed the highest activity compared to controls and 0.17 mol/L NaCl-treated cultures. Based on these observations it was suggested that salt treatment resulted in increased protein disulphide formation and thioredoxin and glutaredoxin systems are important regulators of this process, strongly involved in salt stress response. The highest activity at 0.085 mol/L NaCl may be also related to the regulatory mechanisms, involved in the potentiating of somatic embryogenesis at this salt concentration.
Sub-species diversity of Bulgarian and Macedonian strains from pepper.
Vancheva T, Stoyanova M, Tatyozova M … +2 more , Bogatzevska N, Moncheva P
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019547 · Full text
Sub-species diversity of pepper populations of in Bulgaria and Macedonia in 2012 was the object of this study. Species determination of 44 strains was performed by molecular methods using two pairs of species-specific p...
Sub-species diversity of pepper populations of in Bulgaria and Macedonia in 2012 was the object of this study. Species determination of 44 strains was performed by molecular methods using two pairs of species-specific primers and RFLP (restriction fragment length polymorphism) analysis of the 16S-23S ITS region with II. The populations were characterized by genotypic and phenotypic properties. The genotypic diversity of the strains was evaluated by RAPD (random amplified polymorphic DNA) technique. Primer CUGEA-6 differentiated the strains in two groups, one of which included only Bulgarian strains and revealed a mixed profile of the type strain. Biolog metabolite profiles separated the strains in four groups: two of which were composed only of Bulgarian or Macedonian strains. Correlation between the RAPD and the metabolic profiles was observed. Twelve antibiotics and copper ions in five concentrations (1-5 g kg) were tested for biological activity. The inhibition zones of the Bulgarian strains were statistically proven to be considerably larger than the Macedonian ones in the tests with kanamycin, streptomycin, polymyxin B sulphate, tetracycline and vankomycin. The inhibition zones of the Bulgarian strains were statistically proven to be relatively larger than the Macedonian ones in the copper tests. Based on our studies the Macedonian population of manifested a relative homogeneity while a greater diversity was observed in the Bulgarian population.
Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers.
Tsoktouridis G, Tsiamis G, Koutinas N … +1 more , Mantell S
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26019546 · Full text
Highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. Current methods available for this purpose are tedious, time consu...
Highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. Current methods available for this purpose are tedious, time consuming, highly error prone, expensive, require advanced technical expertise and are sometimes ineffective. We report here the development of a sensitive polymerase chain reaction (PCR) based method for the rapid detection and identification of bacteria occurring in plant tissue cultures. A total of 121 16S ribosomal DNA (rDNA) coding regions from 14 different groups of bacteria, algae and plants, available in the databases, were aligned and several conserved DNA sequences of bacterial origin were identified. From those, five degenerated primers were designed in order to amplify only the bacterial DNA present in mixed plant/bacteria genomic DNA extracts. A known amount of bacterial suspension of either covert or covert were added to plant leaves and total plant/bacterial DNA extracted using three different methods to determine the lowest number of bacteria required to be present in order to allow their detection. The highest sensitivity of the bacterial cell detection was 2.5 × 10 cells of both and inoculums, using template DNA prepared by the method. Generation of PCR amplification fragments was achieved only for the 16S rDNA bacterial gene by using four combinations of degenerated primers. Successive sequence analysis of these amplified fragments led to the rapid detection and molecular identification of bacteria covertly associated with plants.
Humanistic and economic aspects of haemophilia treatment in Bulgaria. Comparison between two therapeutic approaches: prophylactic vs. on-demand treatment.
Petrova G, Tachkov K, Georgieva S … +1 more , Dimitrova M
Biotechnol Biotechnol Equip · 2014 May · PMID 26019545 · Full text
The aim of the present study was to calculate the cost-effectiveness of on-demand and prophylactic treatments of severe haemophilia A for Bulgarian patients. The point of view is that of all patients suffering from sever...
The aim of the present study was to calculate the cost-effectiveness of on-demand and prophylactic treatments of severe haemophilia A for Bulgarian patients. The point of view is that of all patients suffering from severe haemophilia A. An epidemiological model was created, which includes data regarding the number of patients divided into age groups up to 74 years. In the model, the transition age from prophylactic to on-demand treatment was gradually increased to up to 40 years. Costs of blood clotting factor, hospitalization, major surgery and indirect costs were considered; incremental cost-effectiveness ratio was calculated. The results showed that despite the increase in the costs for factor VIII with 20 million BGN, the saving obtained from other health services and indirect expenses reduce the overall expenses with 5.3 million BGN. If there is a gradual increase in the age when patients are transferred from a prophylactic to an on-demand regimen, the costs for factor VIII would increase from 10.4 million to 19.7 million BGN, but due to a decrease in indirect costs as well as other health service costs, the total costs would decrease. The sensitivity analysis showed that the costs for clotting factor VIII are what influences the cost-effectiveness in both regimes. This indicates that decreases in the factor VIII cost will increase the overall efficiency in both regimes. In conclusion, the application of the prophylactic regime for patients up to 40 years of age will provide better treatment, increase the quality of life and decrease the incremental costs.
Adjusting the input ultrasound image data and the atherosclerotic plaque detection in the carotid artery by the FOTOM system.
Ličev L, Tomeček J, Farana R
Biotechnol Biotechnol Equip · 2014 May · PMID 26019544 · Full text
Stroke is the third most frequent cause of death. Specifically, ischemic stroke accounts for the largest group of this kind of cases. Despite all the advances in medical therapeutic methods, no methods that would reliabl...
Stroke is the third most frequent cause of death. Specifically, ischemic stroke accounts for the largest group of this kind of cases. Despite all the advances in medical therapeutic methods, no methods that would reliably reduce mortality from ischemic stroke have been found. Prevention is still the most significant way to combat stroke. When the frequent cause of ischemic stroke is atherosclerotic plaque in the carotid artery, its exploration can help to determine the development of the disease. These problems were very extensively discussed in October 2013 during the XVI International Neurosonology Congress in Sofia organized under the auspices of World Research Neurosonology Group, Bulgarian Neurosonology and Cerebral Hemodynamics Association. Our goal was to develop special modules for carotid artery picture processing (AVI file processing, reparation and reconstruction) and modules containing tools for automated carotid artery plaque detection; and to solve its measurement and three-dimensional modelling of the carotid artery and the plaque. New modules were implemented into the FOTOM system and tested on appropriate input data files, which verified their functionality and applicability.
Production of a new non-specific nuclease from subsp. : optimization of induction conditions using response surface methodology.
Fang XJ, Tang ZX, Li ZH … +2 more , Zhang ZL, Shi LE
Biotechnol Biotechnol Equip · 2014 May · PMID 26019543 · Full text
A new non-specific nuclease from subsp. () was expressed in () BL 21 Star (DE3)plysS. Induction conditions, including isopropyl-β-D-thiogalactoside (IPTG) concentration, cell density (OD600), induction time and induct...
A new non-specific nuclease from subsp. () was expressed in () BL 21 Star (DE3)plysS. Induction conditions, including isopropyl-β-D-thiogalactoside (IPTG) concentration, cell density (OD600), induction time and induction temperature, were optimized using response surface methodology. Statistical analysis of the results revealed that induction temperature and all the quadratic terms of variables had significant effects on enzyme activity of . The optimal induction conditions were as follows: 1.5 mmol/L IPTG, OD600 of 0.80, induction time of 20.5 h, and induction temperature of 32 °C. Under the optimized conditions, the highest enzyme activity could be obtained.
Metal Zn(II), Cu(II), Ni (II) complexes of ursodeoxycholic acid as putative anticancer agents.
Dyakova L, Culita DC, Marinescu G … +4 more , Alexandrov M, Kalfin R, Patron L, Alexandrova R
Biotechnol Biotechnol Equip · 2014 May · PMID 26019542 · Full text
The aim of the study was to evaluate the influence of metal [Zn(II), Cu(II), Ni(II)] complexes with ursodeoxycholic acid (UDCA) on the viability and proliferation of tumour and non-tumour cells. Cell lines established fr...
The aim of the study was to evaluate the influence of metal [Zn(II), Cu(II), Ni(II)] complexes with ursodeoxycholic acid (UDCA) on the viability and proliferation of tumour and non-tumour cells. Cell lines established from retrovirus-transformed chicken hepatoma (LSCC-SF-Mc29) and rat sarcoma (LSR-SF-SR) as well as from human cancers of the breast (MCF-7), uterine cervix (HeLa), lung (A549) and liver (HepG2) were used as model systems. Non-tumour human embryo (Lep-3) cells were also included in some of the experiments. The investigations were carried out by the thiazolyl blue tetrazolium bromide (MTT) test, neutral red uptake cytotoxicity assay, crystal violet staining, double staining with acridine orange and propidium iodide and the colony-forming method. The results obtained revealed that: (1) UDCA and its metal complexes in the tested concentrations decreased (to a varying degree) the viability and proliferation of the treated cells in a time- and concentration-dependent manner; (2) chicken hepatoma (LSCC-SF-Mc29) cells were most sensitive to the cytotoxic and antiproliferative action of the compounds tested, followed by rat sarcoma (LSR-SF-SR) cells; (3) Cu‒UDCA and Ni‒UDCA were more effective against animal LSCC-SF-Mc29 and LSR-SF-SR cells, while Zn‒UDCA significantly decreased the viability and proliferation of human tumour cell lines; (4) applied independently, UDCA expressed lower cytotoxic/cytostatic activity as compared to metal complexes; and (5) the sensitivity of the non-tumour embryonic Lep-3 cells to the effects of UDCA and its metal complexes was comparable or even higher than those of the human tumour cells.
Seroprevalance of Crimean-Congo haemorrhagic fever in Bulgarian livestock.
Barthel R, Mohareb E, Younan R … +7 more , Gladnishka T, Kalvatchev N, Moemen A, Mansour SS, Rossi C, Schoepp R, Christova I
Biotechnol Biotechnol Equip · 2014 May · PMID 26019541 · Full text
Crimean-Congo haemorrhagic fever (CCHF) is a tick-borne zoonotic disease. Over the past decade, CCHF cases in humans have emerged in Turkey and reemerged in the Balkan countries, Ukraine and Tajikistan. Occupational cont...
Crimean-Congo haemorrhagic fever (CCHF) is a tick-borne zoonotic disease. Over the past decade, CCHF cases in humans have emerged in Turkey and reemerged in the Balkan countries, Ukraine and Tajikistan. Occupational contact with infected livestock has been recognized as a common cause of the disease. A cross-sectional seroprevalence study in livestock was conducted in farming communities of an endemic area in Bulgaria, southeastern Europe. Overall, 72% of the tested animals were positive for IgG antibodies to CCHF virus. By the time the animals were one-year old almost 50% had serologic evidence of CCHF infection, and by two years already 80% of them had been infected. The data obtained in this study reflect current situation of CCHF virus infection among livestock in Bulgaria. The results showed active CCHF virus circulation that poses risk for humans to be infected during contacts with animals and requires public health awareness.
Radioprotective effect of hemocyanin in gamma induced acute radiation syndrome.
Kindekov I, Mileva M, Krastev D … +5 more , Vassilieva V, Raynova Y, Doumanova L, Aljakov M, Idakieva K
Biotechnol Biotechnol Equip · 2014 May · PMID 26019540 · Full text
The radioprotective effect of hemocyanin (RtH) against radiation-induced injuries (stomach ulcers, survival time and endogenous haemopoiesis) and post-radiation recovery was investigated in male albino mice (C3H strain)...
The radioprotective effect of hemocyanin (RtH) against radiation-induced injuries (stomach ulcers, survival time and endogenous haemopoiesis) and post-radiation recovery was investigated in male albino mice (C3H strain). Radiation course was in a dose of 7.5 Gy (LD 100/30 - dose that kills 100% of the mice at 30 days) from Cs with a dose of 2.05 Gy/min. Radiation injuries were manifested by inducing а hematopoietic form of acute radiation syndrome. RtH was administered intraperitoneally in a single dose of 50, 100, 150 and 200 mg/kg body weight (b. w.) once a day for five consecutive days before irradiation. The results obtained showed that radiation exposure led to (1) 100% mortality rate, (2) ulceration in the stomach mucosa and (3) decrease formation of spleen colonies as a marker of endogenous haemopoiesis. Administration of RtH at a dose of 200 mg/kg provided better protection against radiation-induced stomach ulceration, mitigated the lethal effects of radiation exposure and recovered endogenous haemopoiesis versus irradiated but not supplemented mice. It could be expected that RtH will find a use in mitigating radiation induced injury and enhanced radiorecovery.
Analysis of stress distribution around total hip stems custom-designed for the standardized Asian femur configuration.
Mu Jung J, Sang Kim C
Biotechnol Biotechnol Equip · 2014 May · PMID 26019539 · Full text
In total hip replacement (THR), bone resorption related to the foreign body reaction around the implant causes bonding failure at the bone-prosthesis interface and adversely affects the function and longevity of femoral...
In total hip replacement (THR), bone resorption related to the foreign body reaction around the implant causes bonding failure at the bone-prosthesis interface and adversely affects the function and longevity of femoral implants. Stress shielding is thought to be one of the possible biomechanical factors that causes bone resorption, and is related to prosthesis design. We therefore investigated stress distribution at the bone-implant interface of implant models custom-fitted to Asian individuals, using a finite-element method. Based on the standard geometry of Asian femurs, we designed four different custom-fitted implant stems and applied boundary conditions, including a stationary loading of 1750 N. Even though stress shielding was observed for all four different prostheses, the custom-designed implant with a stepped groove in the proximal-medial region had the largest maximum principal stress distribution along paths on the bone-implant interface. This implant type also showed the highest maximum principal stress distribution at the proximal (0.308 MPa), mid (0.872 MPa) and distal (12.981 MPa) regions of the cortical surface of the femur. In conclusion, the implant design with a stepped groove in the proximal-medial region showed an overall increase in stress distribution due to minimization of stress shielding afforded by the reduced effective area in the bone-implant interface. Therefore, this hip implant type could be a possible geometry to remain functional over the long term in THR patients.
Whole genome methylation analyses of schizophrenia patients before and after treatment.
Rukova B, Staneva R, Hadjidekova S … +3 more , Stamenov G, Milanova V, Toncheva D
Biotechnol Biotechnol Equip · 2014 May · PMID 26019538 · Full text
The aetiology of schizophrenia is still unknown but it involves both heritable and non-heritable factors. DNA methylation is an inheritable epigenetic modification that stably alters gene expression. It takes part in the...
The aetiology of schizophrenia is still unknown but it involves both heritable and non-heritable factors. DNA methylation is an inheritable epigenetic modification that stably alters gene expression. It takes part in the regulation of neurodevelopment and may be a contributing factor to the pathogenesis of brain diseases. It was found that many of the antipsychotic drugs may lead to epigenetic modifications. We have performed 42 high-resolution genome-wide methylation array analyses to determine the methylation status of 27,627 CpG islands. Differentially methylated regions were studied with samples from 20 Bulgarian individuals divided in four groups according to their gender (12 males/8 females) and their treatment response (6 in complete/14 in incomplete remission). They were compared to two age and sex matched control pools (110 females in female pool/110 males in male pool) before and after treatment. We found significant differences in the methylation profiles between male schizophrenia patients with complete remission and control male pool before treatment (, , , , , , , , ) and male schizophrenia patients with complete remission and control male pool after treatment (, , , , , ) that potentially could be used as target genes for new therapeutic strategies as well as markers for good treatment response. Our data revealed major differences in methylation profiles between male schizophrenia patients in complete remission before and after treatment and healthy controls which supports the hypothesis that antipsychotic drugs may play a role in epigenetic modifications.
VEGF expression, microvessel density and dendritic cell decrease in thyroid cancer.
Gulubova M, Ivanova K, Ananiev J … +4 more , Gerenova J, Zdraveski A, Stoyanov H, Vlaykova T
Biotechnol Biotechnol Equip · 2014 May · PMID 26019537 · Full text
Thyroid cancer is one of the five most common cancers in the age between 20 and 50 years. Many factors including the potent angiogenic vascular endothelial growth factor (VEGF) and different dendritic cell types are know...
Thyroid cancer is one of the five most common cancers in the age between 20 and 50 years. Many factors including the potent angiogenic vascular endothelial growth factor (VEGF) and different dendritic cell types are known to be related to thyroid tumourogenesis. The study was performed to address the expression of VEGF and microvessel density in thyroid cancers and to evaluate the effect of VEGF expression in thyroid tumour cells on the dendritic cells. We investigated 65 patients with different types of thyroid carcinomas: papillary (PTC), oncocytic (OTC), follicular (FTC) and anaplastic (ATC), immunohistochemically with antibodies against VEGF, CD1a, CD83, S100 and CD31. Our results suggest that the expression of VEGF is significantly more often in PTC than ATC (92.3% vs. 60.0%, = 0.025). The microvessel density marked with CD31 in the tumour border of PTC was significantly higher as compared to FTC ( = 0.039), but not to ATC and OTC ( = 0.337 and 0.134). We found that CD1a- and CD83-positive cells were dispersed with variable density and in OC CD31 vessel numbers were positively correlated with CD83 dendritic cells in tumour stroma ( = 0.847, = 0.016). We did not find statistically significant associations of the survival of patients with PTC after the surgical therapy with VEGF expression and MVD. In conclusion we may state that VEGF expression in tumour cells of thyroid cancer can induce neovascularization and suppress dendritic cells.
Evaluation of prognostic utility of MIB-1 and p53 expression in pituitary adenomas: correlations with clinical behaviour and follow-up results.
Hadzhiyanev A, Ivanova R, Nachev E … +6 more , Elenkova A, Yaneva M, Zaharieva S, Marinov M, Surchev J, Ivanova A
Biotechnol Biotechnol Equip · 2014 May · PMID 26019536 · Full text
Pituitary adenomas (PAs) show a broad clinicomorphological spectrum. The proliferation activity, evaluated by MIB-1 labelling index (LI), and p53 expression have been pointed as predictive markers for invasiveness and pr...
Pituitary adenomas (PAs) show a broad clinicomorphological spectrum. The proliferation activity, evaluated by MIB-1 labelling index (LI), and p53 expression have been pointed as predictive markers for invasiveness and progression. The aim of this study was to evaluate the proliferation rate and p53 expression and to look for any relationships with the clinical behaviour and follow-up results in a series of Bulgarian patients with PAs. A total of 93 patients with PAs (81 hormone-secreting, 12 non-functioning), who were operated on and followed up for a period of five years, were included. The MIB-1 LI and p53 expressions were determined by immunohistochemistry and correlated with various clinical and tumour variables. The whole group of PAs showed a low proliferation rate with evident variations in a small number of cases (MIB-1 LI - 0.50 ± 0.56, from 0.1 to 3.30). MIB-1 LI correlated with tumour size ( = 0.012) and was positively related with male gender ( = 0.23) and partial surgical resection ( = 0.036). We found no significant differences regarding the age, functional activity, invasion ( = 33), expansion ( = 37) and tumour recurrences (seven cases). Only 10 cases (10.8%) showed a focal, nuclear p53 immunoreactivity. The p53 positive tumours had higher proliferation rate ( = 0.0001) but no relationship with the other clinical and tumour variables. Among all cases, there was only one case with higher MIB-1 LI (3.3%), positive p53 expression and tumour recurrence after surgery. Our results show that most PAs have a low proliferation rate and lack of p53 expression, as well as no relationship with tumour invasion or postsurgical progression.
High-level production of L-threonine by recombinant with combined feeding strategies.
Wang J, Cheng LK, Chen N
Biotechnol Biotechnol Equip · 2014 May · PMID 26019535 · Full text
The process of L-threonine production using TRFC was investigated, and the result showed that there was a large amount of acetic acid in the broth. The effects of acetic acid, which is a known inhibitory metabolite in...
The process of L-threonine production using TRFC was investigated, and the result showed that there was a large amount of acetic acid in the broth. The effects of acetic acid, which is a known inhibitory metabolite in cultivation, on L-threonine production by recombinant . TRFC were evaluated, and the result indicated that the growth of . TRFC and L-threonine formation were significantly inhibited in the presence of acetic acid. Two combined feeding strategies were applied to L-threonine fed-batch fermentation in order to investigate the effects of the feeding strategy on L-threonine fermentation. The results showed that using the combined feeding strategy of pseudo-exponential feeding and glucose-stat feeding resulted in high cell density (36.67 g L) and L-threonine production (124.57 g L) as well as low accumulation of by-products. This work provides a useful approach for large-scale production of L-threonine.
Examination of the technological properties of newly isolated strains of the genus and possibilities for their application in the composition of starters.
Denkova R, Ilieva S, Denkova Z … +2 more , Georgieva L, Krastanov A
Biotechnol Biotechnol Equip · 2014 May · PMID 26019534 · Full text
The ability of four strains - LBRZ7 (isolated from fermented cabbage), LBRZ12 (isolated from fermented cabbage), LBRH9 (of human origin) and ssp. LBRC11 (isolated from home-made cheese) - to grow in flour/water env...
The ability of four strains - LBRZ7 (isolated from fermented cabbage), LBRZ12 (isolated from fermented cabbage), LBRH9 (of human origin) and ssp. LBRC11 (isolated from home-made cheese) - to grow in flour/water environment and to accumulate high concentrations of viable cells was examined. Two starters for sourdough were created for lab-scale production of wheat bread: a two-strain starter and a four-strain starter. Wheat bread with improved properties - greater loaf volume, enhanced flavour and softer and brighter crumb - was obtained from the 7% four-strain starter sourdough. The addition of sourdough in the production of wheat bread affected positively the technological and organoleptic characteristics of the final bread by inhibiting the growth of wild yeasts and mold and spores without the addition of preservatives. The inclusion of 15% of the four-strain starter sourdough in the bread-making process led to enhanced safety and longer shelf life of the baked bread.
Decolourization of azo dyes by a newly isolated sp. strain Y3, and effects of various factors on biodegradation.
Cui D, Li G, Zhao M … +1 more , Han S
Biotechnol Biotechnol Equip · 2014 May · PMID 26019533 · Full text
In this study, we isolated and characterized a new strain of sp. Y3, which was capable of decolourizing azo dyes under anaerobic conditions. The effects of physico-chemical parameters on the Methyl Red degradation by th...
In this study, we isolated and characterized a new strain of sp. Y3, which was capable of decolourizing azo dyes under anaerobic conditions. The effects of physico-chemical parameters on the Methyl Red degradation by the strain were determined. The results indicated that strain Y3 exhibited a good decolourization ability in the range of pH from 4 to 9, temperature from 30 °C to 42 °C and salinity from 1% to 4%. A broad spectrum of azo dyes with different structures could be decolourized by the strain. The isolate decolourized Methyl Red, Congo Red, Orange I and Methyl Orange by almost 100% (100 mg/L) in 48 h. The culture exhibited an ability to decolourize repeated additions of dye, showing that the strain could be used for multiple cycles of biodegradation. Azo dyes at high concentrations could be tolerated and degraded by Y3. An almost complete mineralization of Methyl Red and Congo Red at the concentration of 800 mg/L was observed within 48 h. The high degradation potential of this bacterium supports its use in the treatment of industrial wastewater containing azo dyes.
Effect of different carbon sources on the biological phosphorus removal by a sequencing batch reactor using pressurized pure oxygen.
Wei J, Imai T, Higuchi T … +4 more , Arfarita N, Yamamoto K, Sekine M, Kanno A
Biotechnol Biotechnol Equip · 2014 May · PMID 26019532 · Full text
The effect of different carbon source on the efficiency of enhanced biological phosphorus removal (EBPR) from synthetic wastewater with acetate and two ratios of acetate/starch as a carbon source was investigated. Three...
The effect of different carbon source on the efficiency of enhanced biological phosphorus removal (EBPR) from synthetic wastewater with acetate and two ratios of acetate/starch as a carbon source was investigated. Three pressurized pure oxygen sequencing batch reactor (POSBR) experiments were operated. The reactors (POSBR1, POSBR2 and POSBR3) were developed and studied at different carbon source ratios of 100% acetate, 75% acetate plus 25% starch and 50% acetate plus 50% starch, respectively. The results showed that POSBR1 had a higher phosphate release-to-uptake ratio and, respectively, in a much higher phosphorus removal efficiency (93.8%) than POSBR2 (84.7%) and POSBR3 (77.3%) within 30 days of operation. This indicated that the phosphorus removal efficiency decreased the higher the starch concentration was. It was also found that POSBR1 produced more polyhydroxyalkanoates (PHAs) than the other reactors. Based on the effect of the carbon source on the PHA concentration and consumption, the conditions of POSBR1 were favourable for the growth of polyphosphate-accumulating organisms and therefore, beneficial for the biological phosphorus removal process.
Long-term assessment of the self-purification potential of a technologically managed ecosystem: the Middle Iskar cascade.
Lincheva S, Todorova Y, Topalova Y
Biotechnol Biotechnol Equip · 2014 May · PMID 26019531 · Full text
The Middle Iskar cascade is situated along the middle course of the Iskar River (Bulgaria) after the capital city Sofia and has three small hydroelectric power plants that were put into operation by the end of 2012. The...
The Middle Iskar cascade is situated along the middle course of the Iskar River (Bulgaria) after the capital city Sofia and has three small hydroelectric power plants that were put into operation by the end of 2012. The aim of this study was to evaluate the self-purification potential of water in the reservoirs of these plants as an important and necessary condition for their ecological functioning. The assessment was made by hydrochemical parameters (dissolved suspended solids, insoluble suspended solids, total suspended solids, nitrites, nitrates, ammonium, phosphates, chemical oxygen demand, dissolved oxygen) and microbiological parameters (aerobic heterotrophic bacteria and bacteria growing in an Endo medium) and covered a period of three years (2010, 2011 and 2012). Standard methods were applied, mainly colorimetric and microbiological cultivation methods. The obtained results showed high levels of some of the tested indicators during 2012. In the section of the Middle Iskar cascade a high self-purification potential was observed in the reservoirs which maintain good water quality.
Efficacious rat model displays non-toxic effect with Korean beechwood creosote: a possible antibiotic substitute.
Quynh AN, Sharma N, Cho KK … +7 more , Yeo TJ, Kim KB, Jeong CY, Min TS, Young KJ, Kim JN, Jeong DK
Biotechnol Biotechnol Equip · 2014 May · PMID 26019530 · Full text
Wood creosote, an herbal anti-diarrheal and a mixture of major volatile compounds, was tested for its non-toxicological effects, using a rat model, with the objective to use the creosote as an antibiotic substitute. A to...
Wood creosote, an herbal anti-diarrheal and a mixture of major volatile compounds, was tested for its non-toxicological effects, using a rat model, with the objective to use the creosote as an antibiotic substitute. A total of 30 Sprague-Dawley rats were studied to form five groups with 6 rats each. Korea beechwood creosote was supplemented into three test groups with 0.03 g/kg, 0.07 g/kg and 0.1 g/kg body weight/day without antibiotic support, along with a positive control of Apramycin sulphate (at 0.5% of the daily feed) and a negative control. Korean beechwood creosote supplementation showed no negative effect on the body weight gain in comparison to the negative and the positive control groups and the feed conversion ratio was also comparable with that of the control groups. The clinical pathology parameters studied were also under the umbrella of normal range, including liver specific enzymes, blood glucose, total protein, blood urea nitrogen (BUN), which indicated no toxic effect of creosote at the given doses. The non-hepatotoxic effect was also confirmed using hepatic damage specific molecular markers like Tim-p1, Tim-p2 and Tgf-β1. The results suggested that Korean beechwood may be used as antibiotic substitute in weanling pigs feed without any toxic effect on the body. Although the antimicrobial properties of creosote were not absolutely similar to those of apramycin sulphate, they were comparable.
Reverse transcriptase domain sequences from tree peony () long terminal repeat retrotransposons: sequence characterization and phylogenetic analysis.
Guo DL, Hou XG, Jia T
Biotechnol Biotechnol Equip · 2014 May · PMID 26019529 · Full text
Tree peony is an important horticultural plant worldwide of great ornamental and medicinal value. Long terminal repeat retrotransposons (LTR-retrotransposons) are the major components of most plant genomes and can substa...
Tree peony is an important horticultural plant worldwide of great ornamental and medicinal value. Long terminal repeat retrotransposons (LTR-retrotransposons) are the major components of most plant genomes and can substantially impact the genome in many ways. It is therefore crucial to understand their sequence characteristics, genetic distribution and transcriptional activity; however, no information about them is available in tree peony. Ty1--like reverse transcriptase sequences were amplified from tree peony genomic DNA by polymerase chain reaction (PCR) with degenerate oligonucleotide primers corresponding to highly conserved domains of the Ty1--like retrotransposons in this study. PCR fragments of roughly 270 bp were isolated and cloned, and 33 sequences were obtained. According to alignment and phylogenetic analysis, all sequences were divided into six families. The observed difference in the degree of nucleotide sequence similarity is an indication for high level of sequence heterogeneity among these clones. Most of these sequences have a frame shift, a stop codon, or both. Dot-blot analysis revealed distribution of these sequences in all the studied tree peony species. However, different hybridization signals were detected among them, which is in agreement with previous systematics studies. Reverse transcriptase PCR (RT-PCR) indicated that Ty1- retrotransposons in tree peony were transcriptionally inactive. The results provide basic genetic and evolutionary information of tree peony genome, and will provide valuable information for the further utilization of retrotransposons in tree peony.
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