Hu D, Zhang P, Sun YL
… +3 more, Zhang S, Wang Z, Chen C
Biotechnol Biotechnol Equip
· 2014 May · PMID 26019528
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Ten universal primer pairs of the plant chloroplast genome were used to amplify the chloroplast DNA (cpDNA) non-coding regions in eight mulberry ( spp.) genotypes, including , , , and . Subsequently, the polymerase chai...Ten universal primer pairs of the plant chloroplast genome were used to amplify the chloroplast DNA (cpDNA) non-coding regions in eight mulberry ( spp.) genotypes, including , , , and . Subsequently, the polymerase chain reaction (PCR) products were digested by seven restriction enzymes and the D-T fragment for sequence alignment, and the variations were expected to provide the genetic information for system classification. The results from this study showed that: (1) 10 cpDNA primer pairs could be used for successful amplification in the tested materials, with approximately 17.1 kb of the chloroplast genome analysed. The 152 marker loci were detected by 70 primer/restriction endonuclease combinations, among which the D-T non-coding region digested by I, I, I and I was detected by visible fragment length variation in different genotypes of the genus . (2) eight L. genotypes were divided into two groups based on the digesting pattern discrepancy through cpDNA. The genotypes displayed diversity on an intraspecies level. 'Nongsang No.12' was identical with the female parent 'Beiqu No.1' () in the surveyed sequence, but different from the male parent 'Tongxiangqing' (), suggesting that the cpDNA was maternal inheritance in L. (3) There were two deletion fragments (451-456 bp; 840-863bp) and six base point mutations in the D-T region based on homologous sequence alignment. The sequence of D-T in the cpDNA of mulberry could provide more genetic information for phylogenetic analysis and pedigree identification.
Biotechnol Biotechnol Equip
· 2014 May · PMID 26019527
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It has been demonstrated that the lectin from (BanLec-1) could inhibit the cellular entry of human immunodeficiency virus (HIV). In order to evaluate its effects on tobacco mosaic virus (TMV), the gene was cloned and t...It has been demonstrated that the lectin from (BanLec-1) could inhibit the cellular entry of human immunodeficiency virus (HIV). In order to evaluate its effects on tobacco mosaic virus (TMV), the gene was cloned and transformed into and tobacco, respectively. Recombinant BanLec-1 showed metal ions dependence, and higher thermal and pH stability. Overexpression of in tobacco resulted in decreased leaf size, and higher resistance to TMV infection, which includes reduced TMV cellular entry, more stable chlorophyll contents, and enhanced antioxidant enzymes. BanLec-1 was found to bind directly to the TMV capsid protein , and to inhibit TMV infection in a dose-dependent manner. In contrast to limited prevention , purified rBanLec-1 exhibited more significant effects on TMV infection . Taken together, our study indicated that BanLec-1 could prevent TMV infection in tobacco, probably through the interaction between BanLec-1 and TMV capsid protein.
Vachev T, Ivanova D, Yahubyan G
… +3 more, Naimov S, Minkov I, Gozmanova M
Biotechnol Biotechnol Equip
· 2014 May · PMID 26019526
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Potato spindle tuber viroid (PSTVd) is an infectious small, circular, non-coding single-stranded RNA that induces disease on many crop species, ornamental plants, weeds and parasitic plants. PSTVd propagate in their host...Potato spindle tuber viroid (PSTVd) is an infectious small, circular, non-coding single-stranded RNA that induces disease on many crop species, ornamental plants, weeds and parasitic plants. PSTVd propagate in their host as a population of closely related but non-identical RNA variants referred to as quasispecies. Recently, we have described three arising PSTVd variants in the parasitic plant after mechanical inoculation with the PSTVd KF440-2 isolate. These derived mutants were designated as G241-C, C208-U and C227-U PSTVd variants. Each of these variants carries a single-nucleotide substitution compared to the PSTVd KF440-2 sequence from which they are considered to have evolved. Here we complement our previous studies on these mutants by exploring their potential to infect the floral organs of tomato plants. We found that the PSTVd G241-C and C208-U variants were able to replicate in systemic leaves and floral organs of tomato plants, while the PSTVd C227-U variant did not develop systemic infection. Furthermore, we analysed the progeny of these PSTVd variants in sepals and petals of tomato plants for retention of the specific mutations.
Biotechnol Biotechnol Equip
· 2014 May · PMID 26019525
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A hundred and five years ago, Albert Calmette and Camille Guérin began a daunting task, which is unmatched even today, that led to the most widely used vaccine in human history. Despite a century of scientific advances,...A hundred and five years ago, Albert Calmette and Camille Guérin began a daunting task, which is unmatched even today, that led to the most widely used vaccine in human history. Despite a century of scientific advances, BCG (an acronym for Bacillus Calmette-Guérin) remains the only vaccine for prevention of tuberculosis. Due to the fact that the use of BCG vaccines will continue, either as a stand-alone or as a prime vaccine in prime-boost immunization strategies, the World Health Organization (WHO) has underlined the necessity for further work toward better characterization, evaluation and quality control of the BCG vaccine, taking into account recent advances in genetics and molecular biology. The potential benefit of such improved characterization could be addressed to better and easier differentiation between sub-strains used by different manufacturers. It may help to ensure consistency of production in terms of genetic stability and it may also help the clinical evaluation of new antituberculosis vaccines. Last but not least, the state-of-the-art technologies could facilitate the quality control performed by the manufacturers and by National Control Authorities as well.
Biotechnol Biotechnol Equip
· 2014 May · PMID 26019524
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The uneventful progression through the cell cycle is closely associated with the rhythm set by the circadian clock machinery, with the S-phase of the cell cycle typically occurring at night. Presence of unrepaired DNA da...The uneventful progression through the cell cycle is closely associated with the rhythm set by the circadian clock machinery, with the S-phase of the cell cycle typically occurring at night. Presence of unrepaired DNA damage may reset the phase of the circadian clock, providing opportunities for damage assessment, repair and/or the induction of pro-apoptotic pathways. The core proteins of the circadian clock regulate directly or indirectly a significant number of genes coding for proteins involved in checkpoint transition, cell proliferation and programmed cell death. Disruption of the circadian rhythm may increase the risk for some multifactorial diseases and conditions, including glucose intolerance, cardiovascular disease and various common cancers. In patients with cancer, chronic circadian misalignment may stimulate the growth of tumours and may modify the outcomes of anticancer therapy. Knowledge about the role of physiological rhythms in human disease may contribute to the field of individualized medicine, specifically, in risk assessment and prognostication of the outcomes in patients with multifactorial disease.
Biotechnol Biotechnol Equip
· 2014 Nov · PMID 26740785
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A signal transformation system of posture movement for the stable state was designed in order to measure the bio-physical signal. To estimate the subject in a stable state on the basis of the bio-physical signal in the p...A signal transformation system of posture movement for the stable state was designed in order to measure the bio-physical signal. To estimate the subject in a stable state on the basis of the bio-physical signal in the posture movement, the conditions of vision, vestibular, somatosensory apparatus and the central nervous system (CNS) were detected. Based on the vision condition, there was a greater average variation (Vi-α and Vi-α) in position with eyes closed and eyes opened (PC and PO), which was 27.11 ± 6.36 units. The vestibular condition showed a slightly larger average variation (Ve-α and Ve-α) in the body position with PC and PO, which was 6.60 ± 1.65 units. The somatosensory condition demonstrated a lower average variation (So-α and So-α) in position with PC and PO that amounted to 3.653 ± 2.424 units. The CNS condition was confirmed to indicate very little average variation (C-α and C-α) in body position with PC and PO that was at 0.401 ± 0.56 units. As the model depends on the bio-physical transformation system of posture movement, the average values of these perturbations were computed (0.01-2 Hz, range of Fourier frequency). The system consists of a data algorithm, an acquisition system, a data signal processing unit and a network system for the evaluated stability.
Ghareeb DA, ElAhwany AM, El-Mallawany SM
… +1 more, Saif AA
Biotechnol Biotechnol Equip
· 2014 Nov · PMID 26740784
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In this study we investigated the phytoconstituents , and , and then assessed their possible biological activities by using standard methods. A preliminary phytochemical investigation of the three extracts revealed the...In this study we investigated the phytoconstituents , and , and then assessed their possible biological activities by using standard methods. A preliminary phytochemical investigation of the three extracts revealed the presence of alkaloids, flavonoids, proteins, lipids, phenolic compounds, saponins, sterols and amino acids. Three extracts showed anti-oxidant effect as they inhibited the 1,1-diphenyl-2-picryl hydrazyl (DPPH) oxidation and production of thiobarbituric acid reactive substances (TBARS). Moreover, three extracts showed anti-acetylcholiesterase (AChE) and this effect was concentration dependent. was the most potent inhibitor of AChE. Furthermore, the three plant extracts had an inhibitory effect toward α-glucosidase. The inhibitory effect was concentration dependent and the most potent inhibitor for α-glucosidase was the extract from showed anti-inflammatory effect at tested concentrations while the other two extracts exhibited this effect only at concentration of 25 μg/mL. Finally, had a significant effect against pathogenic bacteria (, sp., and ) in comparison to other extracts from sp., or sp. In conclusion, all tested extracts could be promising sources for the treatment of diabetes, Alzheimer's disease, infectious diseases and oxidative stress related disorders because they are rich in phenols and flavonoids that give anti-oxidant molecules and produce an inhibitory effect against the tested enzymes.
Chaoui I, Atalhi N, Sabouni R
… +4 more, Akrim M, Abid M, Amzazi S, ElMzibri M
Biotechnol Biotechnol Equip
· 2014 Nov · PMID 26740783
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One of the greatest threats to global tuberculosis (TB) control is the growing prevalence of drug resistant strains. In the past decades, considerable efforts have been made upon the development of new molecular technolo...One of the greatest threats to global tuberculosis (TB) control is the growing prevalence of drug resistant strains. In the past decades, considerable efforts have been made upon the development of new molecular technologies and methodologies for detection of drug resistance in (MTB). A sensitive, specific reverse line blot assay, called rifoligotyping (RIFO), for the detection of genotypic resistance to rifampicin (RIF), was designed and evaluated. RIFO includes oligonucleotide probes specific for wild-type and mutant sequences, allowing specific and sensitive detection of both genotypes in a single assay. The RIFO was applied on 500 MTB isolates from Morocco. The results of the RIFO showed a good sensitivity (90.9%) and high specificity (100%); the positive and negative predictive values were 100% and 96.1%, respectively. This rapid, simple, economical assay provides a practical alternative for RIF genotyping, especially in low-income countries, to improve TB control and management.
Wahab RA, Basri M, Rahman RN
… +4 more, Salleh AB, Rahman MB, Chaibakhsh N, Leow TC
Biotechnol Biotechnol Equip
· 2014 Nov · PMID 26740782
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Most substrate for esterification has the inherent problem of low miscibility which requires addition of solvents into the reaction media. In this contribution, we would like to present an alternative and feasible option...Most substrate for esterification has the inherent problem of low miscibility which requires addition of solvents into the reaction media. In this contribution, we would like to present an alternative and feasible option for an efficient solvent-free synthesis of menthyl butyrate using a novel thermostable crude T1 lipase. We investigated the effects of incubation time, temperature, enzyme loading and substrate molar ratio and determined the optimum conditions. The high conversion of menthyl butyrate catalyzed by crude T1 lipase in a solvent-free system is greatly affected by temperature and time of the reaction media. The highest yield of menthyl butyrate was 99.3% under optimized conditions of 60 °C, incubation time of 13.15 h, 2.53 mg, 0.43% (w/w) enzyme to substrate ratio and at molar ratio of butyric anhydride/menthol 2.7:1. Hence, the investigation revealed that the thermostable crude T1 lipase successfully catalyzed the high-yield production of menthyl butyrate in a solvent-free system. The finding suggests that the crude T1 lipase was a promising alternative to overcome shortcomings associated with solvent-assisted enzymatic reactions.
Biotechnol Biotechnol Equip
· 2014 Nov · PMID 26740781
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The pathways for synthesis of 5-aminolevulinic acid (5-ALA) use either succinyl-CoA and glycine (C-4 pathway), or glutamate (C-5 pathway). Although synthesizes 5-ALA through the C-4 pathway, it also has the genes coding...The pathways for synthesis of 5-aminolevulinic acid (5-ALA) use either succinyl-CoA and glycine (C-4 pathway), or glutamate (C-5 pathway). Although synthesizes 5-ALA through the C-4 pathway, it also has the genes coding for the enzymes of the C-5 pathway, except for glutamyl-tRNA reductase. The glutamyl-tRNA reductase gene was cloned from and expressed in ; thus, the C-5 pathway was enabled to function upon assembling all the required genes. Consequently, a new and unique bacterial strain producing more 5-ALA was developed. Biohydrogen was also produced in the same bioprocess within a biorefinery approach using sugar beet molasses as substrate. The amount of 5-ALA produced by the modified strain was 25.9 mg/g dry cell weight (DCW), whereas the wild-type strain produced 12.4 mg/g DCW. In addition, the amount of H generated by the modified and wild-type cells, respectively, was 0.92 L/L culture and 1.05 L/L culture.
Wang D, Qin Y, Han J
… +5 more, Zhang L, Xu X, Liu X, Wang C, Liu X
Biotechnol Biotechnol Equip
· 2014 Nov · PMID 26740780
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Rice blast resistance () genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes w...Rice blast resistance () genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) genes , , , and than in NB and KHS. of abscisic acid (ABA) signalling may be involved in the interaction, including , , and . However, was shown to negatively regulate interaction. and were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that and played an important role in the mediated response. It was shown that could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while and could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.
Ranawake AL, Manangkil OE, Yoshida S
… +3 more, Ishii T, Mori N, Nakamura C
Biotechnol Biotechnol Equip
· 2014 Nov · PMID 26740779
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Cold tolerance is an important breeding target in rice production. We studied quantitative trait loci (QTLs) controlling cold tolerance at germination (CTG) and early seedling (CTS) stages, using recombinant inbred lines...Cold tolerance is an important breeding target in rice production. We studied quantitative trait loci (QTLs) controlling cold tolerance at germination (CTG) and early seedling (CTS) stages, using recombinant inbred lines derived from a × cross. CTG was evaluated based on the percentage rate of germination at 15 °C for 12 days after pre-incubation of imbibed seeds at 20 °C for 2 days. For CTS, seven-day-old seedlings grown at 25 °C were subjected to two consecutive periods of three-day cold stress at 4 °C with an intervening eight-day recovery at 25 °C. CTS evaluation was according to an arbitrary five-point rating system at the fifth day of recovery after each stress period. No correlations were found between CTG and CTS, while a weak correlation was detected between CTS after the first and second stress. By the composite interval mapping, five QTLs for CTG explaining 5.7%-9.3% of the total phenotypic variance (PVE) and nine for CTS with PVE of 5.8%-35.6% were detected. Only one of these QTLs was common, indicating growth-stage specificity of cold tolerance. Four of the five QTLs after the second cold stress were different from the ones after the first cold stress. Analysis of variance test showed significant interactions between alleles at the QTL sites and the two stress conditions with respect to the mean CTS scores. A possible involvement of cold acclimation and usefulness of germplasms in breeding for cold tolerance in rice was discussed.
Biotechnol Biotechnol Equip
· 2014 Sep · PMID 26740778
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In this paper, the functional state modelling approach is validated for modelling of the cultivation of two different microorganisms: yeast () and bacteria (). Based on the available experimental data for these fed-batch...In this paper, the functional state modelling approach is validated for modelling of the cultivation of two different microorganisms: yeast () and bacteria (). Based on the available experimental data for these fed-batch cultivation processes, three different functional states are distinguished, namely primary product synthesis state, mixed oxidative state and secondary product synthesis state. Parameter identification procedures for different local models are performed using genetic algorithms. The simulation results show high degree of adequacy of the models describing these functional states for both and cultivations. Thus, the local models are validated for the cultivation of both microorganisms. This fact is a strong structure model verification of the functional state modelling theory not only for a set of yeast cultivations, but also for bacteria cultivation. As such, the obtained results demonstrate the efficiency and efficacy of the functional state modelling approach.
Georgieva E, Petrova D, Yordanova Z
… +3 more, Kapchina-Toteva V, Cellarova E, Chaneva G
Biotechnol Biotechnol Equip
· 2014 Sep · PMID 26740777
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Antioxidative activity of two cultivated species - Boiss. and Fr. - was estimated after cryopreservation. Both species were successfully regenerated after a cryopreservation procedure performed by the vitrification m...Antioxidative activity of two cultivated species - Boiss. and Fr. - was estimated after cryopreservation. Both species were successfully regenerated after a cryopreservation procedure performed by the vitrification method. did not manifest any significant oxidative stress-induced changes caused by low-temperature treatment. Conversely, a decrease in green pigments' content of was measured, particularly pronounced in chlorophyll , which was accompanied by an increase of carotenoids in the regenerated plants. A strong increase of malone dialdehyde and HO levels in tissues was detected. Superoxide dismutase activity was enhanced by 170%, as well as the catalase activity, which was 220% above the control. The same trend was observed in , although less pronounced - 143% increase of superoxide dismutase and 112% of catalase. Cryopreservation did not influence the phenol content in the examined plants, but it led to an increase of flavonoid content, especially in , by 237%. Total antioxidant activity in regenerated varied around the control level, but it was increased in . The free proline content in remained almost unaffected after freezing, as opposed to , where a strong increase of proline content (208% above the control) occurred. An electrolyte leakage from the cells of regenerated after cryopreservation was also registered, albeit not significant.
Biotechnol Biotechnol Equip
· 2014 Sep · PMID 26740776
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Buch.-Ham. ex D. Don, an important antibacterial source of Chinese traditional medicine, has a widespread distribution in a few ecological habitats of China. We generated a full-length complementary DNA (cDNA) library fr...Buch.-Ham. ex D. Don, an important antibacterial source of Chinese traditional medicine, has a widespread distribution in a few ecological habitats of China. We generated a full-length complementary DNA (cDNA) library from a sample of elite individuals with superior antibacterial properties, with satisfactory parameters such as library storage (4.30 × 10 CFU), efficiency of titre (1.30 × 10 CFU/mL), transformation efficiency (96.35%), full-length ratio (64.00%) and redundancy ratio (3.28%). The BLASTN search revealed the facile formation of counterparts between the experimental sample and in view of high-homology cDNA sequence (90.79%) with -values <1 - 50. Sequence similarities to known proteins indicate that the entire sequences of the full-length cDNA clones consist of the major of functional genes identified by a large set of microarray data from the present experimental material. For other Compositae species, a large set of full-length cDNA clones reported in the present article will serve as a useful resource to facilitate further research on the transferability of expressed sequence tag-derived simple sequence repeats (EST-SSR) development, comparative genomics and novel transcript profiles.
Khiyami MA, Almoammar H, Awad YM
… +2 more, Alghuthaymi MA, Abd-Elsalam KA
Biotechnol Biotechnol Equip
· 2014 Sep · PMID 26740775
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Plant diseases are among the major factors limiting crop productivity. A first step towards managing a plant disease under greenhouse and field conditions is to correctly identify the pathogen. Current technologies, such...Plant diseases are among the major factors limiting crop productivity. A first step towards managing a plant disease under greenhouse and field conditions is to correctly identify the pathogen. Current technologies, such as quantitative polymerase chain reaction (Q-PCR), require a relatively large amount of target tissue and rely on multiple assays to accurately identify distinct plant pathogens. The common disadvantage of the traditional diagnostic methods is that they are time consuming and lack high sensitivity. Consequently, developing low-cost methods to improve the accuracy and rapidity of plant pathogens diagnosis is needed. Nanotechnology, nano particles and quantum dots (QDs) have emerged as essential tools for fast detection of a particular biological marker with extreme accuracy. Biosensor, QDs, nanostructured platforms, nanoimaging and nanopore DNA sequencing tools have the potential to raise sensitivity, specificity and speed of the pathogen detection, facilitate high-throughput analysis, and to be used for high-quality monitoring and crop protection. Furthermore, nanodiagnostic kit equipment can easily and quickly detect potential serious plant pathogens, allowing experts to help farmers in the prevention of epidemic diseases. The current review deals with the application of nanotechnology for quicker, more cost-effective and precise diagnostic procedures of plant diseases. Such an accurate technology may help to design a proper integrated disease management system which may modify crop environments to adversely affect crop pathogens.
Angelova T, Rangelova N, Dineva H
… +2 more, Georgieva N, Müller R
Biotechnol Biotechnol Equip
· 2014 Jul · PMID 26740774
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Antibacterial SiO hybrid materials based on tetraethyl orthosilicate (TEOS), hydroxypropylmethylcellulose (HPMC) and silver were prepared by the sol-gel method. The content of cellulose derivate was 5 wt% and the silver...Antibacterial SiO hybrid materials based on tetraethyl orthosilicate (TEOS), hydroxypropylmethylcellulose (HPMC) and silver were prepared by the sol-gel method. The content of cellulose derivate was 5 wt% and the silver concentration varied from 0.5 wt% to 2.5 wt%. The amorphous nature, morphology and antibacterial behaviour were studied. Fourier transform infrared (FTIR) spectra of the hybrids showed characteristic peaks for SiO network. Scanning electron microscope (SEM) analysis confirmed the formation of spherically shaped silver nanoparticles with a size of 30 nm on the matrix surfaces. and K12 were used as model microorganisms. The hybrid materials demonstrated bacteriostatic and bactericidal effect on the tested bacteria. Highest sensitivity to the obtained hybrids was observed in with significant lag-phase delay and biggest inhibition zone sizes.
Evstatieva Y, Yordanova M, Chernev G
… +2 more, Ruseva Y, Nikolova D
Biotechnol Biotechnol Equip
· 2014 Jul · PMID 26740773
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Bioencapsulation of microbial cells in silica-based matrices has proved to be a good strategy to enhance the biosynthetic capabilities and viability of bioproducers. In the present study, mycelium and pellet cultures of...Bioencapsulation of microbial cells in silica-based matrices has proved to be a good strategy to enhance the biosynthetic capabilities and viability of bioproducers. In the present study, mycelium and pellet cultures of strain PP were successfully immobilized in sol-gel hybrid matrices composed of tetraethylorthosilicate as an inorganic precursor, 5% (w/v) starch and 10 or 15% (w/v) polyethylene oxide, or 10% (w/v) calcium alginate as organic compounds. Biosynthetic activity of immobilized cultures was investigated by batch and fed-batch cultivation and the obtained results of 3042.04 IU cm were comparable with the enzyme activity of the free cell culture. Immobilized cultures retained their viability and biosynthetic capabilities up to the 744th h during fed-batch fermentation processes. Consequently, sol-gel encapsulation in hybrid matrices could be considered as a promising technique for immobilization of PP in order to increase the α-amylase production.
Daskalova D, Kukov A, Kirilova I
… +1 more, Ivanova-Kicheva M
Biotechnol Biotechnol Equip
· 2014 Jul · PMID 26740772
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The present study aimed to investigate the effect of different seminal plasma proteins (SPPs) on boar spermatozoa functional characteristics. We investigated the putative protective effect of SSPs on sperm cells motility...The present study aimed to investigate the effect of different seminal plasma proteins (SPPs) on boar spermatozoa functional characteristics. We investigated the putative protective effect of SSPs on sperm cells motility and velocity, as well as on the integrity of the plasma membrane (PM) during low-temperature storage at 4 °C. SPPs fractions were obtained and purified by gel permeation chromatography (GPC). Nine fractions of SPPs were obtained and further characterized by 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Sperm computer analysis (SCA) after incubation of spermatozoa with separated proteins revealed that fraction 6 consisting of low molecular weight (MW) proteins could preserve spermatozoa motility and velocity better when compared to those with higher MW. Two-dimensional (2D) elecrtophoretic analysis showed that fraction 6 contained proteins with the following MW and isoelectric point (pI): 16 kDa and pI 7.35, 18 kDa and pI 5.20, 19 kDa and pI 7.35, 26 kDa and pI 4.50, 26 kDa and pI 4.30, 29 kDa and pI 5.85.
Dağ A, Tules Fırat E, Uysal E
… +2 more, Ketani Ş, Aydın Ketani M
Biotechnol Biotechnol Equip
· 2014 Jul · PMID 26740771
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This study was aimed at the histological investigation of the impact of experimental diabetes on the healthy gingiva of rats. Thirty male Wistar rats were used in this study. The animals were randomly divided into two gr...This study was aimed at the histological investigation of the impact of experimental diabetes on the healthy gingiva of rats. Thirty male Wistar rats were used in this study. The animals were randomly divided into two groups ( = 15) prior to the experiment. Group 1 experimental diabetes was created by streptozotocin injection in 15 rats. Group 2 comprised the control group (15 rats). On the 7th, 14th and 21st days after the induction of diabetes by streptozotocin, five animals from each group were euthanized by cardiac puncture. The gingiva of the maxillary left first molar tooth of the sacrificed animals was extracted for histological examination. Histological examination demonstrated that, when compared to the control group, the diabetes group displayed marked hyperkeratosis and parakeratosis of the gingival epithelium on day 21 post-induction. Furthermore, the diabetes group presented with an increased number of inflammatory cells and vasodilatation of the capillaries, in comparison to the controls. The overall evaluation of the findings obtained in this study suggested that diabetes alone could cause changes in the periodontium and affect periodontal health.