Biotechnology, Biotechnological Equipment[JOURNAL]
Sun
200 papers
New X-chromosomal interactors of dFMRP regulate axonal and synaptic morphology of brain neurons in .
Georgieva D, Dimitrov R, Kitanova M … +1 more , Genova G
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26740770 · Full text
Fragile X syndrome is a neuro-developmental disease caused by transcriptional inactivation of the gene FMR1 (fragile X mental retardation 1) and loss of its protein product FMRP. FMRP has multiple neuronal functions whic...
Fragile X syndrome is a neuro-developmental disease caused by transcriptional inactivation of the gene FMR1 (fragile X mental retardation 1) and loss of its protein product FMRP. FMRP has multiple neuronal functions which are implemented together with other proteins. To better understand these functions, the aim of this study was to reveal new protein interactors of dFMRP. In a forward genetic screen, we isolated ethyl-metanesulphonate-induced X-chromosomal modifier mutations of 1. Four of them were identified and belong to the genes: , , and . They are dominant suppressors of the 1 overexpression wing phenotype 'notched wings'. These mutations dominantly affected the axonal and synaptic morphology of the lateral ventral neurons (LNv's) in adult brains. Heterozygotes for each of them displayed effects in the axonal growth, pathfinding, branching and in the synapse formation of these neurons. Double heterozygotes for both 1-null mutation and for each of the suppressor mutations showed robust genetic interactions in the fly central nervous system. The mutations displayed severe defects in the axonal growth and synapse formation of the LNv's in adult brains. Our biochemical studies showed that neither of the proteins - Rok, Shaggy, Peb/Hnt or Ras - encoded by the four mutated genes regulates the protein level of dFMRP, but dFMRP negatively regulates the protein expression level of Rok in the brain. Altogether, these data suggest that Rok, Shaggy, Peb/Hnt and Ras are functional partners of dFMRP, which are required for correct wing development and for neuronal connectivity in brain.
Hexavalent chromium reduction by chromate-resistant haloalkaliphilic sp. M-Cr newly isolated from tannery effluent.
Mabrouk ME, Arayes MA, Sabry SA
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26740769 · Full text
The current study aimed to isolate and characterize a chromate-resistant bacterium from tannery effluent, able to reduce Cr(VI) aerobically at high pH and salinity. Environmental contamination by hexavalent chromium, Cr(...
The current study aimed to isolate and characterize a chromate-resistant bacterium from tannery effluent, able to reduce Cr(VI) aerobically at high pH and salinity. Environmental contamination by hexavalent chromium, Cr(VI), presents a serious public health problem. Enrichment led to the isolation of 12 bacteria displaying different degrees of chromate reduction. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparison indicated that the most potent strain belonged to the genus . The new strain designated as sp. M-Cr was able to reduce 82% of 50 mg L Cr(VI) in 48 h, concomitant with discolouring of yellow colour of the medium and formation of white insoluble precipitate of Cr(III). It exhibited growth up to 3500 mg L Cr(VI), 20% NaCl and showed strong Cr(VI) reduction under alkaline condition, pH 10. Scanning electron microscopy revealed precipitation of chromium hydroxide on bacterial cell surfaces, which showed characteristic peak of chromium in energy-dispersive X-ray analysis. Plackett-Burman design was used to evaluate the influence of related parameters for enhancing Cr(VI) reduction. Glucose, yeast extract and KHPO were confirmed as significant variables in the medium. Data suggest sp. M-Cr as a promising candidate for bioremediation of Cr(VI) contaminated effluents particularly in saline and alkaline environments. Up to our knowledge, this is the first report on isolation of haloalkaliphilic sp. from tannery effluent.
Modelling of risk effect of mercury on nutrient transformation in lake sediments.
Lincheva S, Schneider I, Daskalova E … +1 more , Topalova Y
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26740768 · Full text
The influence of mercury on the transformation processes in the waters and the sediments in the reservoir of a small hydroelectric power plant (SHPP) Lakatnik was simulated in laboratory conditions. SHPP Lakatnik is the...
The influence of mercury on the transformation processes in the waters and the sediments in the reservoir of a small hydroelectric power plant (SHPP) Lakatnik was simulated in laboratory conditions. SHPP Lakatnik is the first (of nine similar) SHPPs put into exploitation in the middle part of the Iskar River (Bulgaria). In previous studies of the sediments in the reservoir of SHPP Lakatnik, our team found high concentrations of mercury, sometimes exceeding maximum concentration limit (MCL). In model bioreactors we studied the effect of mercury on the dynamics of the following hydrochemical and microbiological indicators: NH, NO, NO, PO, chemical oxygen demand (COD), total organic carbon (TOC); aerobic and anaerobic heterotrophs (AH and AnH), spp. (Ps.), spp. (Ac.), sulphate-reducing bacteria (SR), denitrifying microorganisms (Dn). We looked for linear correlations among the studied indicators in order to find quick, mutual replaceability among them. The extent to which mercury affects the amount of key microbial groups and the rate of transformation of biogenic elements was calculated in percentages. The microorganism dynamics showed that AH, AnH and Ps. adapt more quickly and their number increased more in the presence of mercury, whereas SR, Dn and Ac. were inhibited to a greater extent by the presence of mercury. Mercury had a marked stimulating effect on Ps., which showed a 312% increase in number at the 192nd hour. The obtained results can be used when decisions have to be taken in risky situations related to increased concentrations of mercury in the sediments.
Insights into the stereospecificity of the d-specific dehalogenase from sp. RC1 toward d- and l-2-chloropropionate.
Sudi IY, Hamid AA, Shamsir MS … +3 more , Jamaluddin H, Wahab RA, Huyop F
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26740767 · Full text
Halogenated compounds are recalcitrant environmental pollutants prevalent in agricultural fields, waste waters and industrial by-products, but they can be degraded by dehalogenase-containing microbes. Notably, 2-haloalka...
Halogenated compounds are recalcitrant environmental pollutants prevalent in agricultural fields, waste waters and industrial by-products, but they can be degraded by dehalogenase-containing microbes. Notably, 2-haloalkanoic acid dehalogenases are employed to resolve optically active chloropropionates, as exemplified by the d-specific dehalogenase from sp. RCI (DehD), which acts on d-2-chloropropionate but not on its l-enantiomer. The catalytic residues of this dehalogenase responsible for its affinity toward d-2-chloropropionate have not been experimentally determined, although its three-dimensional crystal structure has been solved. For this study, we performed docking and molecular dynamic simulations of complexes formed by this dehalogenase and d- or l-2-chloropropionate. Arg134 of the enzyme plays the key role in the stereospecific binding and Arg16 is in a position that would allow it to activate a water molecule for hydrolytic attack on the d-2-chloropropionate chiral carbon for release of the halide ion to yield l-2-hydroxypropionate. We propose that within the DehD active site, the NH group of Arg134 can form a hydrogen bond with the carboxylate of d-2-chloropropionate with a strength of ∼4 kcal/mol that may act as an acid-base catalyst, whereas, when l-2-chloropropionate is present, this bond cannot be formed. The significance of the present work is vital for rational design of this dehalogenase in order to confirm the involvement of Arg16 and Arg134 residues implicated in hydrolysis and binding of d-2-chloropropionate in the active site of d-specific dehalogenase from sp. RC1.
SRAP analysis on genetic relationships of genotypes in the genus Mill.
Xu R, Hu D, Chen Z … +3 more , Zhang P, Jiang X, Tang G
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26740766 · Full text
Thirteen sequence-related amplified polymorphism (SRAP) primers combined with polymerase chain reaction (PCR) were used to evaluate the genetic relationships among 24 genotypes of the genus Mill. including Sect. , , an...
Thirteen sequence-related amplified polymorphism (SRAP) primers combined with polymerase chain reaction (PCR) were used to evaluate the genetic relationships among 24 genotypes of the genus Mill. including Sect. , , and . Out of 115 surveyed fragments, 107 (93.04%) were polymorphic. Coefficients of genetic similarity ranged from 0.538 to 0.868, with an average value of 0.720 between pairs of materials, which indicated the high degree of polymorphism within this species. Cluster analysis showed that all genotypes were divided into six groups. A dendrogram showed that some of the clustered genotypes were largely congruent for geographical distribution. The species in Sect. , and were separated to a great extent. However, the DNA patterns for some genotype groups did not demonstrate relative agreement in their pedigrees. In Sect. , was independently separated, while were clustered in group III with , and classified into Ser. . Five species originated from the American region were dispersed into two groups within the dendrogram. The conflicts were reflecting their complex genetic backgrounds.
Therapeutic approaches to the treatment of recurrent respiratory papillomatosis of the aerodigestive tract (a clinical study).
Avramov T, Vetckova E, Nikolova M … +5 more , Valev D, Manolova A, Tafradgiiska M, Kostadinov D, Tchalacov I
Biotechnol Biotechnol Equip · 2014 Jul · PMID 26692782 · Full text
Recurrent respiratory papillomatosis (RRP) is a rare disease, characterized by recurrent proliferation of benign squamous cell papillomas in the larynx as well as in the other parts of the aerodigestive tract. We have co...
Recurrent respiratory papillomatosis (RRP) is a rare disease, characterized by recurrent proliferation of benign squamous cell papillomas in the larynx as well as in the other parts of the aerodigestive tract. We have compared different treatment options for RRP of the aerodigestive tract including surgical, conservative and combined approaches. A total of 43 patients with papillomatosis that received a combined therapy were followed in the period from 2009 to 2013. The treatment included electrosurgery and CO laser surgery alongside with either immunotherapy with Bacillus Calmette-Guerin (BCG) (Calgevax) or α-interferon. In the control group without immunotherapy ( = 16) we used conventional microlaryngeal surgery. During the follow-up, relapse occurred in two patients for the CO laser surgery with Calgevax immunotherapy group ( = 16). In the group treated with α-interferon preceded by CO laser surgery ( = 9) and electrosurgery ( = 2), relapse had occurred in three patients. Among the control group, recurrence was observed in six patients. This required re-operation. Our data showed a three times more frequent relapses among patients who were operated with conventional surgery as compared to those operated with CO laser surgery and Calgevax immunotherapy, and two times more often relapses in patients operated with conventional surgery as compared to those with electrosurgery and CO laser surgery and application of α-interferon therapy. Conventional and laser surgeries have a palliative effect, though playing an important role in ensuring the airway patency. While specific antivirus treatment for human papilloma viruses does not exist, the immune modulation with Calgevax considerably reduces the frequency of relapses, by stimulating cellular immune effector mechanisms. The combined protocol allows rarefication of relapses and improvement of patients' quality of life, but not complete healing.
Study on anti-inflammatory and immunomodulatory effects of clomipramine in carrageenan- and lipopolysaccharide-induced rat models of inflammation.
Kostadinov I, Delev D, Petrova A … +4 more , Stanimirova I, Draganova K, Kostadinova I, Murdjeva M
Biotechnol Biotechnol Equip · 2014 May · PMID 26740765 · Full text
The aim of the present study was to evaluate the anti-inflammatory effect of clomipramine in carrageenan- and lipopolysaccharide-induced (LPS-induced) models of inflammation by investigating the changes in serum levels o...
The aim of the present study was to evaluate the anti-inflammatory effect of clomipramine in carrageenan- and lipopolysaccharide-induced (LPS-induced) models of inflammation by investigating the changes in serum levels of the pro-inflammatory cytokine TNF-α and the anti-inflammatory cytokines IL-10 and TGF-β after single and repeated administration of the drug. In order to study the effect of single and repeated doses of clomipramine on carrageenan-induced paw oedema, male Wistar rats were divided in five groups ( = 8): control, positive control group and three experimental groups treated with 5, 10 and 20 mg/kg bw clomipramine, respectively. The effect of single and repeated doses of clomipramine on serum cytokine levels was studied as animals were divided in four groups: two control groups treated with saline and two experimental groups treated with clomipramine 20 mg/kg bw. Carrageenan and LPS were injected immediately after clomipramine or saline injection. Serum cytokine concentrations were tested by enzyme immunoassay. Following acute administration only the highest dose that was used inhibited the carrageenan-induced inflammation. Oedema inhibition was observed with 5, 10 and 20 mg/kg bw clomipramine after repeated administration. Single and repeated administration of clomipramine at a dose of 20 mg/kg bw did not significantly change the serum levels of TGF-1β, IL-10 and TNF-α when compared to the controls in carrageenan-induced inflammation. Following LPS-induced inflammation clomipramine significantly increased the serum levels of TGF-1β after repeated administration and decreased TNF-α in rats after single-dose and repeated pretreatment with 20 mg/kg bw clomipramine. A significant increase in the levels of IL-10 in relation to this inflammatory model was observed only in single dose treated animals. Clomipramine possesses an anti-inflammatory effect in the carrageenan-induced model of exudative inflammation. In LPS-induced inflammation, clomipramine showed an immunomodulatory effect, decreasing TNF-α and increasing TGF-1β after repeated administration, and increasing IL-10 after a single dose.
Factors influencing the trans-membrane transport of -octadecane by sp. DG17.
Hua F, Wang HQ, Zhao YC
Biotechnol Biotechnol Equip · 2014 May · PMID 26740764 · Full text
In soil bioremediation techniques, the trans-membrane transport of hydrocarbons across the cell membrane is a new and complex point of understanding the process of hydrocarbons biodegradation. In this study, the effect o...
In soil bioremediation techniques, the trans-membrane transport of hydrocarbons across the cell membrane is a new and complex point of understanding the process of hydrocarbons biodegradation. In this study, the effect of different environmental factors, including substrate concentration, bacterial inoculums, pH, salinity, substrate analogues and nutrients, on the transport of [C]-octadecane by sp. DG17 was investigated. The results showed that cellular [C]-octadecane levels increased along with the increase in the substrate concentration. However, the trans-membrane transport of [C]-octadecane was a saturable process in the case of equal amounts of inoculum (biomass). The highest concentration of accumulated [C]-octadecane was 0.51 μmol mg ± 0.028 μmol mg after incubation for 20 min. Meanwhile, the cellular -octadecane concentration decreased along with the biomass increase, and reached a stable level. Acidic/alkaline conditions, high salinity, and supplement of substrate analogues could inhibit the transport of [C]-octadecane by sp. DG17, whereas nitrogen or phosphorus deficiency did not influence this transport. The results suggested that trans-membrane transport of octadecane depends on both the substrate concentration and the microorganism biomass, and extreme environmental conditions could influence the biodegradation ability of microorganisms through inhibiting the transport of extracellular octadecane.
Tomato () variety discrimination and hybridization analysis based on the 5S rRNA region.
Sun YL, Kang HM, Kim YS … +4 more , Baek JP, Zheng SL, Xiang JJ, Hong SK
Biotechnol Biotechnol Equip · 2014 May · PMID 26740763 · Full text
The tomato () is a major vegetable crop worldwide. To satisfy popular demand, more than 500 tomato varieties have been bred. However, a clear variety identification has not been found. Thorough understanding of the phylo...
The tomato () is a major vegetable crop worldwide. To satisfy popular demand, more than 500 tomato varieties have been bred. However, a clear variety identification has not been found. Thorough understanding of the phylogenetic relationship and hybridization information of tomato varieties is very important for further variety breeding. Thus, in this study, we collected 26 tomato varieties and attempted to distinguish them based on the 5S rRNA region, which is widely used in the determination of phylogenetic relations. Sequence analysis of the 5S rRNA region suggested that a large number of nucleotide variations exist among tomato varieties. These variable nucleotide sites were also informative regarding hybridization. Chromas sequencing of Yellow Mountain View and Seuwiteuking varieties indicated three and one variable nucleotide sites in the non-transcribed spacer (NTS) of the 5S rRNA region showing hybridization, respectively. Based on a phylogenetic tree constructed using the 5S rRNA sequences, we observed that 16 tomato varieties were divided into three groups at 95% similarity. Rubiking and Sseommeoking, Lang Selection Procedure and Seuwiteuking, and Acorn Gold and Yellow Mountain View exhibited very high identity with their partners. This work will aid variety authentication and provides a basis for further tomato variety breeding.
Isolation and characterization of NBS-LRR resistance gene analogues from mango.
Lei X, Yao Q, Xu X … +1 more , Liu Y
Biotechnol Biotechnol Equip · 2014 May · PMID 26740762 · Full text
The nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene family is a class of genes in plants. NBS genes play a very important role in disease defence. To further study the variation and homology of mango NBS-LR...
The nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene family is a class of genes in plants. NBS genes play a very important role in disease defence. To further study the variation and homology of mango NBS-LRR genes, 16 resistance gene analogues (RGAs) (GenBank accession number HM446507-22) were isolated from the polymerase chain reaction fragments and sequenced by using two degenerate primer sets. The total nucleotide diversity index was 0.362, and 236 variation sites were found among 16 RGAs. The degree of homology between the RGAs varied from 44.4% to 98.5%. Sixteen RGAs could be translated into amino sequences. The high level of this homology in the protein sequences of the P-loop and kinase-2 of the NBS domain between the RGAs isolated in this study and previously characterized genes indicated that these cloned sequences belonged to the NBS-LRR gene family. Moreover, these 16 RGAs could be classified into the non-TIR-NBS-LRR gene family because only tryptophan (W) could be claimed as the final residual of the kinase-2 domain of all RGAs isolated here. From our results, we concluded that our mango NBS-LRR genes possessed a high level of variation from the mango genome, which may allow mango to recognize many different pathogenic virulence factors.
The expression of genes at locus is associated with the level of frost tolerance in Bulgarian winter wheat cultivars.
Todorovska EG, Kolev S, Christov NK … +4 more , Balint A, Kocsy G, Vágújfalvi A, Galiba G
Biotechnol Biotechnol Equip · 2014 May · PMID 26740761 · Full text
The regulation of the majority of cold-regulated genes in plants is mediated by () transcription factor family. Natural differences in frost tolerance (FT) of wheat have been mapped to the () locus on chromosome group...
The regulation of the majority of cold-regulated genes in plants is mediated by () transcription factor family. Natural differences in frost tolerance (FT) of wheat have been mapped to the () locus on chromosome group 5 and are associated with variation in threshold induction temperatures and/or transcript levels of genes. This study used real-time reverse-transcription polymerase chain reaction (qRT-PCR) to compare the relative expression levels of four genes ( and ) in crown tissue of two Bulgarian hexaploid winter wheat cultivars (Milena and Russalka) with distinct levels of low-temperature (LT) tolerance but same vernalization requirement, and the spring cultivar Chinese Spring. The transcription profiles of the selected genes showed that they are induced by cold treatment at 2 °C. Analysis of transcript abundance revealed that the four genes were expressed at higher levels in the frost tolerant Milena than in the susceptible Russalka. Largest differences (fivefold and fourfold) in expression levels between both winter cultivars were observed in two of the analysed genes, and , respectively. The higher steady-state expression levels of genes before the onset of the LT treatment in Milena, combined with stronger induction by cold treatment, suggest that these molecular responses to LT are associated with superior FT development capacity. The results expand our understanding of the molecular mechanisms underlying LT acclimation in Bulgarian wheat and can be used for development of functional markers for improvement of FT wheat-breeding programmes.
Advances in the development of an imaging device for plaque measurement in the area of the carotid artery.
Ličev L, Krumnikl M, Škuta J … +2 more , Babiuch M, Farana R
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740760 · Full text
This paper describes the advances in the development and subsequent testing of an imaging device for three-dimensional ultrasound measurement of atherosclerotic plaque in the carotid artery. The embolization from the ath...
This paper describes the advances in the development and subsequent testing of an imaging device for three-dimensional ultrasound measurement of atherosclerotic plaque in the carotid artery. The embolization from the atherosclerotic carotid plaque is one of the most common causes of ischemic stroke and, therefore, we consider the measurement of the plaque as extremely important. The paper describes the proposed hardware for enhancing the standard ultrasonic probe to provide a possibility of accurate probe positioning and synchronization with the cardiac activity, allowing the precise plaque measurements that were impossible with the standard equipment. The synchronization signal is derived from the output signal of the patient monitor (electrocardiogram (ECG)), processed by a microcontroller-based system, generating the control commands for the linear motion moving the probe. The controlling algorithm synchronizes the movement with the ECG waveform to obtain clear images not disturbed by the heart activity.
Antibacterial and antitumour activities of some plants grown in Turkey.
Usta C, Yildirim AB, Turker AU
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740759 · Full text
Screening of antibacterial and antitumour activities of 33 different extracts prepared with three types of solvents (water, ethanol and methanol) was conducted. The extracts were obtained from 11 different plant species...
Screening of antibacterial and antitumour activities of 33 different extracts prepared with three types of solvents (water, ethanol and methanol) was conducted. The extracts were obtained from 11 different plant species grown in Turkey: L., (Buser) Rothm., Scop., L., Boiss., Wahlenb., L., C. C. Gmelin, Hoffm., L. and Boiss. Antibacterial activity against six bacteria was evaluated: , , , , and by using disc diffusion and well diffusion methods. and were most sensitive to the methanolic extract from . was vulnerable to all used extracts of . In addition, ethanolic or methanolic extracts of , , , , , and displayed strong antibacterial activity against at least one of the tested gram-negative bacteria. The methanolic extract from showed a broad-spectrum activity against both gram-positive and gram-negative bacteria. Antitumour activity was evaluated with -induced potato disc tumour assay. Best antitumour activity was obtained with the aqueous extract from and methanolic extract from (100% and 86% tumour inhibition, respectively).
Long-term effect of radiofrequency turbinoplasty in nasal obstruction.
Akdag M, Dasdag S, Ozkurt FE … +4 more , Celik MY, Degirmenci A, Demir H, Meric F
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740758 · Full text
The aim of this retrospective study is to investigate long term (two years) effect of radiofrequency tissue volume reduction (RFVTR) on nasal obstruction associated with inferior turbinate hypertrophy, which is not respo...
The aim of this retrospective study is to investigate long term (two years) effect of radiofrequency tissue volume reduction (RFVTR) on nasal obstruction associated with inferior turbinate hypertrophy, which is not respond to medical treatment. This retrospective study carried out on 98 patients with nasal obstruction treated by RFVTR (56 males, 42 females, from 17 to 70 years of age). Visual analogue scales (VAS) and nasal endoscopic view score (NES) were used for nasal obstruction to evaluate the efficacy of the treatment. Results of one, three, six, twelve and twenty four months after RFVTR treatment were compared with pre-exposure values. Turbinate edema and nasal obstruction in the treated patients were recovered after one month of treatment ( < 0.01). Maximum improvement were determined at the end of third month ( < 0.01). However, eficacy of RFVTR declined twelve months after treatment. Final percentage of imrovement was found as 51 % at the end of second year of treatment because of co-factors was not eliminated by some patients. On the other hand, no statistical significant difference was observed between the right and left nasal cavity ( < 0.001). In conclusion, the result of this study indicated that patients who have not eliminate co-factors such as smoking, obesity and allergic rhinitis may need re-application of RFTVR. However, further studies on radiofrequecy energy level and elimination of other co-factors are necessary to illuminate the eficacy of RFVTR.
New bacterial isolates from honey bee colonies infected with American foulbrood disease in Egypt.
Masry SH, Kabeil SS, Hafez EE
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740757 · Full text
The American foulbrood disease is widely distributed all over the world and causes a serious problem for the honeybee industry. Different infected larvae were collected from different apiaries, ground in phosphate saline...
The American foulbrood disease is widely distributed all over the world and causes a serious problem for the honeybee industry. Different infected larvae were collected from different apiaries, ground in phosphate saline buffer (PSB) and bacterial isolation was carried out on nutrient agar medium. Different colonies were observed and were characterized biologically. Two bacterial isolates (SH11 and SH33) were subjected to molecular identification using 16S rRNA gene and the sequence analysis revealed that the two isolates are with identity not exceeding 83%. The DNA sequence alignment between the other bacterial strains and the two identified bacterial isolates showed that all the examined bacterial strains have the same ancestor, i.e. they have the same origin. The SH33 isolate was closely related to the isolated from Germany, whereas the isolate SH11 was close to the isolated from India. The phylogenetic tree constructed for 20 different sp. and the two isolates SH11 and SH33 demonstrated that the two isolates are sp. and they are new isolates. The bacterial isolates will be subjected to more tests for more confirmations.
Influence of Ni on urease activity produced by biofilms of 1388.
Marinkova D, Yotova L, Ringeard JM … +1 more , Griesmar P
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740756 · Full text
New TiO-based hybrid materials composed of an organic polymer, cellulose acetate butyrate and copolymer of acrylonitrile acrylamide (AN + AA) were prepared. The effectiveness of immobilization of microbial strain 1388 o...
New TiO-based hybrid materials composed of an organic polymer, cellulose acetate butyrate and copolymer of acrylonitrile acrylamide (AN + AA) were prepared. The effectiveness of immobilization of microbial strain 1388 on the newly synthesized hybrid membranes was investigated by biochemical methods. The obtained results revealed that the matrix more suitable for biofilm formation was composed of organic polymers without a metal component in the membrane composition. The influence of Ni on urease activity produced by biofilms was investigated. The experimental results demonstrated that 2 mg L concentration of Ni in the nutrient medium is more appropriate for biofilm proliferation.
Optimization of medium components for production of chitin deacetylase by Z7, using response surface methodology.
He Y, Xu J, Wang S … +2 more , Zhou G, Liu J
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740755 · Full text
Plackett-Burman design and Box-Behnken response surface methodology (RSM) was employed to optimize the medium components for the chitin deacetylase (CDA) activity from Z7. Plackett-Burman design was applied to determine...
Plackett-Burman design and Box-Behnken response surface methodology (RSM) was employed to optimize the medium components for the chitin deacetylase (CDA) activity from Z7. Plackett-Burman design was applied to determine the specific medium components affecting CDA activity and found that starch, chitin and MgSO were critical in augmenting CDA activity. These significant parameters were further optimized using Box-Behnken RSM and the optimum concentrations of starch, chitin and MgSO were found to be 24.4, 8.8 and 0.19 g/L, respectively. The optimum medium composition was chitin 8.8 g/L, starch 24.4 g/L, yeast extract 10g/L, MgSO 0.19 g/L, KHPO 0.3 g/L and NaCl 5 g/L. Under these optimal conditions, the CDA activity of Z7 increased distinctly from 18.75 to 27.48 U/mL (46.6% increase in total yield).
Investigation of single nucleotide polymorphisms based on the intronic sequences of the propylene alcohol dehydrogenase gene in Chinese tobacco genotypes.
Wei JC, Qiu EJ, Guo HY … +2 more , Hao AP, Chen RP
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740754 · Full text
A pair of primers was designed to amplify the propylene alcohol dehydrogenase gene sequence based on the cDNA sequence of the tobacco allyl-alcohol dehydrogenase gene. All introns were sequenced using traditional polymer...
A pair of primers was designed to amplify the propylene alcohol dehydrogenase gene sequence based on the cDNA sequence of the tobacco allyl-alcohol dehydrogenase gene. All introns were sequenced using traditional polymerase chain reaction (PCR) methods and T-A cloning. The sequences from common tobacco ( L.) and rustica tobacco ( L.) were analysed between the third intron and the fourth intron of the propylene alcohol dehydrogenase gene. The results showed that the alcohol dehydrogenase gene is a low-copy nuclear gene. The intron sequences have a combination of single nucleotide polymorphisms and length polymorphisms between common tobacco and rustica tobacco, which are suitable to identify the different germplasms. Furthermore, there are some single nucleotide polymorphism sites in the target sequence within common tobacco that can be used to distinguish intraspecific varieties.
Identification of activation-tag mutants with altered production of germination stimulants for (L.).
Kirilova I, Denev ID, Bineva R … +4 more , Gevezova M, Alexandrova M, Kostov K, Batchvarova R
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740753 · Full text
Germination of seeds of root parasites like broomrapes () is tightly regulated by chemical products exuded from the roots of the host plant, known as germination stimulants (GSs). Changes in the levels of synthesis and e...
Germination of seeds of root parasites like broomrapes () is tightly regulated by chemical products exuded from the roots of the host plant, known as germination stimulants (GSs). Changes in the levels of synthesis and emission of GS can allow the development of practical measures for control of the crops-harming parasitic species. However, the genes encoding enzymes responsible for GS biosynthesis are still unknown. We performed a large-scale screening of 62,000 activation-tag mutants for alteration in susceptibility to and to identify lines with altered GS production among them. After five successive screenings we identified 36 lines with altered susceptibility to . Seven of them displayed altered levels of GS production. By using a combination of Southern blot and thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR), we pinpointed the location of activation-tag constructs in these lines. A combination of differential display and quantitative real-time PCR (qRT-PCR) allowed us to identify several affected genes. Two of them are directly involved in isoprenoid biosynthetic pathway in chloroplasts, and we believe that their activation led to increased levels of GS production. We believe that these genes are responsible for increased GS production in five of the lines resistant to .
Uptake and trans-membrane transport of petroleum hydrocarbons by microorganisms.
Hua F, Wang HQ
Biotechnol Biotechnol Equip · 2014 Mar · PMID 26740752 · Full text
Petroleum-based products are a primary energy source in the industry and daily life. During the exploration, processing, transport and storage of petroleum and petroleum products, water or soil pollution occurs regularly...
Petroleum-based products are a primary energy source in the industry and daily life. During the exploration, processing, transport and storage of petroleum and petroleum products, water or soil pollution occurs regularly. Biodegradation of the hydrocarbon pollutants by indigenous microorganisms is one of the primary mechanisms of removal of petroleum compounds from the environment. However, the physical contact between microorganisms and hydrophobic hydrocarbons limits the biodegradation rate. This paper presents an updated review of the petroleum hydrocarbon uptake and transport across the outer membrane of microorganisms with the help of outer membrane proteins.
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