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Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal Of Sichuan University. Medical Science Edition[JOURNAL]
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200 papers
[Application of Domestic Robotic Surgical Systems in Gynecologic Surgery].
Peng H, Zheng Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369721 · Full text
In recent years, domestically developed surgical robot systems have rapidly progressed from laboratory research to clinical applications in gynecological surgery, demonstrating multi-dimensional advancement and accelerat...
In recent years, domestically developed surgical robot systems have rapidly progressed from laboratory research to clinical applications in gynecological surgery, demonstrating multi-dimensional advancement and accelerated development. Systems such as Toumai, Jingfeng, Surui, Haishanyi, and MicroHand S have been progressively deployed in both routine and complex procedures, including surgeries for uterine fibroids, ovarian cysts, and gynecologic malignancies such as cervical, endometrial, and ovarian cancers. These systems have established a technology pathway characterized by independent research and development, clinical validation, and expanded indications. They generally offer advantages such as 3D high-definition imaging, highly flexible robotic arms, and precise dissection capabilities, enabling refined operations in the deep pelvis and supporting multi-angle tissue dissection with enhanced visualization, stability, and trauma control. Some systems, including the Surui and Jingfeng SP series, have also demonstrated strong adaptability in single-port and natural orifice (e.g., v-NOTES) approaches. Additionally, 5G-based telesurgery has been preliminarily explored, promoting regional medical collaboration and technology dissemination in underserved areas. Although most domestic systems remain in early-stage clinical adoption with limited large-scale comparative studies and long-term follow-up data, initial outcomes are promising in terms of intraoperative safety, postoperative recovery, and patient experience. However, compared to the Da Vinci system, further evidence is needed regarding their precision in highly complex tumor resections, management of intraoperative complications, and long-term oncological outcomes. Therefore, large-sample, multi-center, prospective studies with long-term follow-up are urgently needed to validate their efficacy and safety and to promote standardized application in gynecological oncology.
[The Relationship Between Interleukin-4, C-reactive Protein, Atopic Dermatitis, and the Therapeutic Effect of Dupilumab].
Zhao T, Wu Y, Shi Z … +2 more , Liu L, Yuan Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369720 · Full text
OBJECTIVE: To analyze the relationship between interleukin-4 (IL-4), C-reactive protein (CRP), and the incidence, severity, and therapeutic efficacy of Dupilumab in atopic dermatitis. METHODS: A total of 106 patients wit...
OBJECTIVE: To analyze the relationship between interleukin-4 (IL-4), C-reactive protein (CRP), and the incidence, severity, and therapeutic efficacy of Dupilumab in atopic dermatitis. METHODS: A total of 106 patients with atopic dermatitis treated from February 2022 to November 2023 were retrospectively selected as the study group, and 50 healthy volunteers from the same period were selected as the control group. The levels of IL-4 and CRP were measured at different stages (acute, subacute, chronic) and severities (mild, moderate, severe) in both the study and control groups. Receiver operating characteristic (ROC) curves were used to analyze the diagnostic value of IL-4 and CRP in atopic dermatitis. Spearman analysis was used to assess the relationship between the severity of atopic dermatitis and the levels of IL-4 and CRP in patients. All patients with atopic dermatitis received standardized 16-week treatment with pembrolizumab and were divided into effective and ineffective treatment groups. Changes in IL-4 and CRP levels before treatment and at 4, 8, 12, and 16 weeks of treatment were compared, and the correlation between IL-4, CRP, and treatment efficacy at each time point after treatment was analyzed. RESULTS: The levels of IL-4 and CRP were lowest in the chronic phase, higher in the subacute phase, and highest in the acute phase ( < 0.05), with the mild group showing lower levels than the moderate group, and the moderate group lower than the severe group ( < 0.05). Before treatment, the levels of IL-4 and CRP in the study group were (14.88 ± 3.91) pg/mL and (6.07 ± 3.02) mg/L, respectively, both significantly higher than those in the control group, which were (8.27 ± 1.09) pg/mL and (1.06 ± 0.31) mg/L ( < 0.05). The areas under the curve for IL-4, CRP, and their combined diagnosis of atopic dermatitis were 0.831 (95% CI: 0.763-0.886), 0.776 (95% CI: 0.702-0.839), and 0.882 (95% CI: 0.821-0.928), respectively ( < 0.05). Spearman analysis showed that IL-4 and CRP levels in patients with atopic dermatitis were positively correlated with disease severity ( = 0.643, 0.659, < 0.05). As treatment time increased, IL-4 and CRP levels gradually decreased, with a statistically significant difference between the effective and ineffective groups ( < 0.05). At all time points before and after treatment, IL-4 and CRP levels in the effective treatment group were lower than those in the ineffective treatment group ( < 0.05). Spearman analysis also showed that IL-4 and CRP levels in patients with atopic dermatitis were positively correlated with treatment efficacy at 12 weeks ( = 0.267, 0.280, < 0.05) and 16 weeks ( = 0.214, 0.377, < 0.05). CONCLUSION: IL-4 and CRP are correlated to the occurrence and development of atopic dermatitis. After treatment with Dupilumab, the levels of IL-4 and CRP in patients significantly decreased. IL-4 and CRP have diagnostic value for atopic dermatitis.
[Microbiological Characteristics of Extrapulmonary Nontuberculous Mycobacterial Infections in a Tertiary Hospital].
Zhou D, Xiong L, Deng J … +1 more , Xiao Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369719 · Full text
OBJECTIVE: To analyze the microbiological characteristics of extrapulmonary nontuberculous mycobacterial (ENTM) infections in a tertiary hospital and to provide data to support clinical diagnosis and treatment of ENTM in...
OBJECTIVE: To analyze the microbiological characteristics of extrapulmonary nontuberculous mycobacterial (ENTM) infections in a tertiary hospital and to provide data to support clinical diagnosis and treatment of ENTM infections. METHODS: A retrospective analysis was conducted on mycobacterial culture results from West China Hospital, Sichuan University from 2020 to 2023. A total of 68 confirmed cases of ENTM infection were enrolled, with all of which meeting the diagnostic criteria for extrapulmonary infection outlined in the . Demographic data, strain distribution, and drug susceptibility test results were analyzed. Microbial species identification was performed using mass spectrometry, and drug susceptibility was assessed using the microbroth microdilution method. RESULTS: Among the 68 ENTM cases, the male-to-female ratio was 1 to 1.2, with individuals aged 40-59 years being the main affected group population. The most common sites of infection were the skin and soft tissues. The primary specimen source was pus (33.8%, 23 cases), followed by pathological tissues (26.5%, 18 cases). The Department of Infectious Diseases was the main source of confirmed cases (29.4%, 20 cases). Among the 68 EMTM cases, rapid-growing mycobacteria (RGM) accounted for 32.4% (22 cases), with being the predominant species, while slowly-growing mycobacteria (SGM) accounted for 67.6% (46 cases), with and being the predominant species. RRGM exhibited higher minimum inhibitory concentration (MICs) for rifampicin and rifabutin but lower MICs for clarithromycin and amikacin. In contrast, SGM demonstrated low MICs for most antibiotics tested. CONCLUSION: In this study, ENTM infections most commonly involve the skin and soft tissues, with SGM being the predominant pathogens and being the dominant species. RGM shows susceptibility to clarithromycin and amikacin, which provides a basis for empirical anti-infective therapy. Although SGM exhibits low MICs to most tested antimicrobial agents, combination regimens remain necessary in clinical practice, and the risk of treatment failure should be thoroughly evaluated.
[The Oral Microbiome: Maintenance of Homeostasis, Disease Associations, and Mechanisms of Pathogenesis].
Chen Z, Zhang X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369718 · Full text
The oral microbiome is a complex and highly structured ecosystem composed of diverse microorganisms, including bacteria, fungi, viruses, and other microbes, which establish an intimate symbiotic relationship with the hos...
The oral microbiome is a complex and highly structured ecosystem composed of diverse microorganisms, including bacteria, fungi, viruses, and other microbes, which establish an intimate symbiotic relationship with the host. Its composition across distinct ecological niches, such as teeth and mucosa, is modulated by multiple factors, including age, genetics, and lifestyle. A stable microbial community acts as an essential barrier for sustaining oral and systemic health. This review systematically examines the structure and function of the oral microbiome under healthy and diseased conditions, with an emphasis on the formation mechanisms of plaque biofilms and their pivotal roles in the initiation and progression of dental caries and periodontitis. Dental caries is predominantly driven by acidogenic and aciduric bacteria, such as and , accompanied by microenvironmental acidification and enamel demineralization. Periodontitis is closely associated with the enrichment of pathogenic microorganisms, including the "red complex" in subgingival plaque, and host immune dysregulation. Furthermore, ecological dysbiosis of the oral microbiome, particularly the abnormal proliferation of pathogens such as and , not only contributes to the development and progression of oral squamous cell carcinoma but also closely correlates with numerous systemic disorders, including cardiovascular diseases, diabetes mellitus, rheumatoid arthritis, and pancreatic cancer, via mechanisms such as inflammatory induction, immunosuppression, and microbial translocation. Systematic elucidation of the ecological characteristics and pathogenic mechanisms of the oral microbiome will provide a critical theoretical foundation for maintaining oral microecological homeostasis and for preventing and treating oral and systemic comorbidities.
[The Role of Salivary Microbiota in Oral and Systemic Disease Development and Diagnosis].
Huang J, Gong T, Zhou X … +1 more , Zheng X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369717 · Full text
The salivary microbiome plays a crucial role in both oral health and systemic diseases, offering significant insights into disease development and early diagnosis. Under normal conditions, a balanced relationship exists...
The salivary microbiome plays a crucial role in both oral health and systemic diseases, offering significant insights into disease development and early diagnosis. Under normal conditions, a balanced relationship exists between the microbiota and the host; however, when this balance is disrupted, it can lead to the onset of oral diseases such as dental caries, periodontal disease, and oral cancer. Changes in the salivary microbiome provide valuable information for the early diagnosis of oral diseases. Moreover, microbial dysbiosis in the oral cavity may promote the translocation of pathogenic microorganisms via the gastrointestinal tract, lungs, or bacteremia, leading to ectopic colonization outside the oral cavity and contributing to the onset and progression of systemic diseases such as colorectal cancer, cardiovascular diseases, and autoimmune disorders. Therefore, this review summarizes the role of the salivary microbiome in disease development and diagnosis, based on recent advancements in research on salivary microbiota, offering new perspectives for the early prevention and clinical management of systemic diseases.
[Study of the Sealing and Antibacterial Properties of Bioceramic Root Canal Sealers Modified With Quaternary Ammonium Salt].
Ma J, Li B, Cheng L
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369716 · Full text
OBJECTIVE: To modify the non-antibacterial bioceramic root canal sealant iRoot SP with the quaternary ammonium salt dimethylaminododecyl methacrylate (DMADDM) and to develop a new type of sealant. METHODS: The cytotoxici...
OBJECTIVE: To modify the non-antibacterial bioceramic root canal sealant iRoot SP with the quaternary ammonium salt dimethylaminododecyl methacrylate (DMADDM) and to develop a new type of sealant. METHODS: The cytotoxicity of the modified root canal sealant was evaluated using the cell counting kit-8 (CCK-8). The solubility and flowability of the sealant were tested according to International Organization for Standardization (ISO) methods. The sealing ability and penetrability of the sealant were assessed by micro-computed tomography (micro-CT) and confocal laser scanning microscope (CLSM). The antibacterial activity of the sealant against biofilm was evaluated by colony-forming unit (CFU) counting, crystal violet staining, scanning electron microscope (SEM), and live/dead staining. RESULTS: When the DMADDM mass fraction was 0.625%, the modified sealant demonstrated good biocompatibility ( > 0.0125). The flowability (> 17 mm) and solubility (< 3%) of the sealant modified with 0.625% DMADDM met ISO standards and showed no significant difference compared with iRoot SP. DMADDM modification did not affect the sealing ability or penetrability of the sealant at different positions of the tooth root ( > 0.05). Antibacterial experiments showed that the modified sealant reduced the CFU count (0.518 ± 0.333 vs. 8.156 ± 0.011, < 0.001) and the amount of biofilm (mean difference of 86.94%, 95% confidence interval: 74.82%-99.07%, < 0.0001) of biofilm at the unaged stage. However, after 1 week of aging (7.327 ± 0.068 vs. 7.422 ± 0.035) or 4 weeks of aging (7.479 ± 0.065 vs. 7.581 ± 0.071), the CFU count of the modified sealant showed no significant difference from the iRoot SP group ( > 0.05). Live/dead staining and SEM also showed that the modified sealant could inhibit the formation of biofilm. CONCLUSION: iRoot SP modified with 0.625% DMADDM exhibited good biocompatibility, sealing ability, and penetrability, and significantly improved resistance to biofilm, providing a new approach for the antibacterial modification of bioceramic root canal sealants.
[Current Applications and Future Prospects of Laparoendoscopic Single-Site Surgery in Urology].
Wei Y, Zhu Q
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369715 · Full text
In recent years, laparoendoscopic single-site surgery (LESS) has gained growing attention because, compared with conventional multiport laparoscopy, it offers superior cosmetic outcomes, reduced postoperative pain, and f...
In recent years, laparoendoscopic single-site surgery (LESS) has gained growing attention because, compared with conventional multiport laparoscopy, it offers superior cosmetic outcomes, reduced postoperative pain, and faster. However, LESS is technically more challenging and characterized by a steep learning curve. The introduction of robotic surgical systems offers new solutions to overcome the technical limitations of LESS, thereby promoting its further development. This article provides a review of the current applications of LESS in urology, with a focus on the technical progress, clinical outcomes, and challenges of robot-assisted LESS (R-LESS). Additionally, the future trends involving the integration of cutting-edge technologies such as artificial intelligence, telesurgery, and medical big data are discussed.
[Applying Robot-Assisted Single-Port Laparoscopic Surgery in Gynecology: Technological Paradigm Innovations and Clinical Advances].
Yang F, Zheng Y
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369714 · Full text
With the continuous development of robot-assisted single-port laparoscopic surgery in gynecology, its indications have gradually expanded from benign gynecologic diseases to malignant tumors, reflecting an emerging trend...
With the continuous development of robot-assisted single-port laparoscopic surgery in gynecology, its indications have gradually expanded from benign gynecologic diseases to malignant tumors, reflecting an emerging trend toward the integration of robotic technology and single-port minimally invasive surgery. This review primarily discusses the clinical application of robotic systems such as the da Vinci Xi platform and domestically developed single-port robotic systems in various complex surgical procedures, as well as the development of technical strategies for optimizing instrument layout and overcoming operative limitations. From a technical perspective, the use of 3-dimensional high-definition visualization, flexible wristed instrumentation, and tremor filtration, combined with innovative surgical paradigms, has effectively addressed the problem of instrument interference in single-port surgery, thereby enabling precise resection, rapid postoperative recovery, and superior cosmetic outcomes with minimal visible scarring. Although dedicated single-port systems still face technical challenges and long-term outcome data remain limited, the future integration of artificial intelligence and remote technologies will further validate their clinical efficacy and promote the standardized application of this approach in gynecological clinical practice.
[From Theory to Governance: The Disciplinary Construction of Chinese Medical Ethics and the Legalization of Ethical Governance].
Liang H, Wang L
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369713 · Full text
This article focuses on the legal transformation of medical ethics in China during the new era of socialism with Chinese characteristics, since the 18th National Congress of the Communist Party of China. By employing dia...
This article focuses on the legal transformation of medical ethics in China during the new era of socialism with Chinese characteristics, since the 18th National Congress of the Communist Party of China. By employing diachronic review and normative analysis, it systematically presents the internal logic and practical pathways of the field's evolution from fragmented self-regulation to integrated governance. Academically, the discipline has advanced through four stages: theoretical foundation and foreign introduction, practical shift and institutional exploration, critical reflection and systemic optimization, and legal-led, full-coverage advancement. Legally, a refined regulatory system has been established, centered on national laws and supported by specialized review regulations. In governance, a transition from passive response to proactive shaping has been achieved, with ethical review expanding across all domains of life science research, and procedural management and accountability fully incorporated into the rule of law. Looking ahead, it is essential to balance institutional rigidity with practical flexibility, contribute ethical wisdom to the Healthy China initiative, and offer a Chinese solution to global science and technology ethics governance.
[Characteristics of the Pathogenic Bacteria Isolated from the Lower Respiratory Tract of the Patients With Acute Exacerbation of Chronic Obstructive Pulmonary Disease].
Wang Y, Wu C, Jiang Z … +2 more , Deng J, He C
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369712 · Full text
OBJECTIVE: To analyze the species distribution and in vitro susceptibility of pathogenic bacteria isolated from the lower respiratory tract of the patients with acute exacerbation of chronic obstructive pulmonary disease...
OBJECTIVE: To analyze the species distribution and in vitro susceptibility of pathogenic bacteria isolated from the lower respiratory tract of the patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD), and to provide guidance for the treatment of pulmonary infections in this kind of patients. METHODS: The AECOPD patients with suspected lower respiratory tract sample infection from January 2015 to December 2024 were included. The data of species identification and in vitro drug susceptibility testing results of pathogenic bacteria isolated from the lower respiratory tract were retrospectively collected. The detection rate, species distribution, in vitro susceptibility of predominant species, and ratios of multidrug-resistant (MDR) bacteria were compared among different sample types. Data were analyzed using WHONET 5.6 and SPSS 26.0 software. RESULTS: A total of 25253 lower respiratory tract samples from 3861 patients were included. The pathogen detection rates in sputum, aspirates and bronchoalveolar lavage fluids were 27.2% (6463/23722), 33.5% (135/403), and 48.9% (551/1128), respectively, with a statistically significant difference of detection rate ( < 0.001). Among the 3,824 non-repetitive strains, the ratio of the isolates recovered from outpatients and emergency patients to those from inpatients is 1∶6, and the ratio of the isolates from intensive care unit patients to those from other units is 1∶7. Gram-negative bacteria accounted for 93.1%, with the predominant species including (23.7%), (21.4%), (12.6%), (11.7%), and (4.6%). Gram-positive bacteria accounted for 6.9%, with was the most common species (3.2%). Fastidious bacteria accounted for 6.4%, including (3.9%), (1.6%), and (0.9%). The detection rate of methicillin-resistant (MRSA) was 41.8%. The detection rates of carbapenem-resistant , , , and were 12.0% (21/175), 18.1% (87/480), 69.9% (635/908), and 29.3% (240/819), respectively. Compared with sputum, non-sputum specimens showed a higher detection rate of MDR bacteria ( < 0.01). CONCLUSION: Gram-negative bacteria were the predominant pathogenic bacteria detected from the low respiratory tract of AECOPD patients, with a low isolation rate of fastidious bacteria. Non-sputum samples exhibited a higher detection rate of MDR bacteria.
[Analysis of Maternal Pregnancy Characteristics and Adverse Neonatal Birth Outcomes at a Single Center From 2017 to 2022].
He Y, Xiong F, Mao M
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369711 · Full text
OBJECTIVE: To analyze changes and correlations in the pregnancy characteristics of parturients and adverse birth outcomes of newborns in a tertiary grade A hospital from 2017 to 2022, and provide a basis for targeted pre...
OBJECTIVE: To analyze changes and correlations in the pregnancy characteristics of parturients and adverse birth outcomes of newborns in a tertiary grade A hospital from 2017 to 2022, and provide a basis for targeted prevention of adverse birth outcomes in newborns. METHODS: Medical records of obstetric deliveries from January 1, 2017, to December 31, 2022, were retrospectively collected. Descriptive analysis was used to examine the pregnancy characteristics of parturients and adverse birth outcomes of newborns. Univariate and multivariate analyses were conducted to explore the relationship between these factors during this period. RESULTS: The final analysis included data from 93547 newborns and 88857 corresponding mothers. From 2017 to 2022, the number of mothers and newborns at our hospital showed an overall increasing trend. The main reproductive age for women in this region was concentrated between 30 and 34 years. The proportion of older mothers (≥ 35 years) showed a general downward trend, and this difference was statistically significant ( = 13.261, < 0.001). Except for cesarean section, the risks of preterm birth, low birth weight (LBW), macrosomia, small for gestational age infant (SGA), and large for gestational age infant (LGA) in this region showed an overall downward trend, with statistically significant differences ( < 0.05). Multivariate logistic regression analysis showed that, compared with mothers under 30 years, the risk of cesarean section for newborns was 1.339 times higher in the 30-34 age group (adjusted odds ratio [aOR] = 1.339, 95% CI: 1.297-1.382) and 2.646 times higher in the ≥ 35 age group (aOR = 2.646, 95% CI: 2.532-2.765). For LGA risk, only mothers aged ≥ 35 years showed an increased risk for newborns (aOR = 1.111, 95% CI: 1.049-1.178, < 0.001). The risks of cesarean section, preterm birth, and macrosomia in the multiparous group were 1.151 times (aOR = 1.151, 95% CI: 1.114-1.189), 1.558 times (aOR = 1.558, 95% CI: 1.486-1.633), and 1.595 times (aOR = 1.595, 95% CI: 1.527-1.688) those in the primiparous group, respectively, while the risk of SGA in the multiparous group was lower than in the primiparous group (aOR = 0.684, 95% CI: 0.655-0.715). The risks of cesarean section, macrosomia, and LGA in the fertilization (IVF) group were 2.295 times (aOR = 2.295, 95% CI: 2.170-2.427), 1.274 times (aOR = 1.274, 95% CI: 1.122-1.447), and 1.300 times (aOR = 1.300, 95% CI: 1.216-1.391) those in the natural conception group, respectively, but the risk of SGA was lower than in the non-IVF group (aOR = 0.774, 95% CI: 0.729-0.821). CONCLUSION: From 2017 to 2022, the characteristics of pregnant women in our hospital changed, and the risk of some adverse neonatal outcomes showed a decreasing trend during this period. Additionally, neonatal birth outcomes among different high-risk groups (women aged 35 or older, multiparous women, and women who conceived with assisted reproductive technology) exhibited distinct characteristics.
[The Effect of Konjac Glucomannan on Blood Glucose and Insulin Levels in Obese Patients With Prediabetes].
Wu R, Tang L, Yang X … +4 more , Ou Q, Gu Y, Yuan L, Tong N
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369710 · Full text
OBJECTIVE: To investigate the effects of konjac glucomannan (KGM) on glycemic indicators and insulin resistance in obese individuals with prediabetes. METHODS: A total of 102 obese individuals diagnosed with prediabetes...
OBJECTIVE: To investigate the effects of konjac glucomannan (KGM) on glycemic indicators and insulin resistance in obese individuals with prediabetes. METHODS: A total of 102 obese individuals diagnosed with prediabetes at the outpatient department of West China Hospital, Sichuan University, between July 2021 and June 2022 were recruited and randomly assigned to either the experimental group (KGM group) or the control group for a three-month trial. All subjects followed personalized dietary and exercise regimens, with the KGM group additionally consuming 60 g of konjac-based dietary fiber biscuits daily to partially replace carbohydrates. This study aimed to examine the impact of KGM on fasting blood glucose, and to explore changes in 2-hour postprandial glucose, insulin-related indicators, and obesity-related parameters in the two groups of subjects. RESULTS: A total of 41 participants in the KGM group completed the trial, with a compliance rate of 77.36%. The control group had 38 completers, with a compliance rate of 77.55%. After three months of dietary and exercise intervention, the effective rate of fasting blood glucose control was 100% in the KGM group, significantly higher than the 82% observed in the control group. The KGM group also had higher effective rates in reducing fasting blood glucose, fasting insulin, insulin resistance, insulin sensitivity, body mass, and waist circumference compared to the control group ( < 0.05). However, there were no statistically significant differences between the two groups in body mass index, 2-hour postprandial blood glucose, 2-hour postprandial insulin, or effective control of pancreatic β-cell activity ( > 0.05). During the trial, two participants in the KGM group experienced severe abdominal discomfort, which resolved after discontinuing the biscuits; two cases reported transient diarrhea, and one case reported increased anal flatulence. CONCLUSION: KGM can effectively control fasting blood sugar and promotes weight loss, lowers blood sugar, and enhances insulin sensitivity in obese individuals with prediabetes.
[Establish a Zebrafish Atherosclerosis Model Screening Platform to Evaluate the Therapeutic Effects of Qi-Regulating and Blood-Activating Prescriptions on Atherosclerosis].
Yin J, Wang H, Wang H … +6 more , Jiang T, Zhang X, Li M, Zhang D, Zhao F, Yang W
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369709 · Full text
OBJECTIVE: To systematically identify traditional Chinese medicine (TCM) compound formulas with anti-atherosclerosis (AS) potential using a screening platform based on multi-strain gene knockout zebrafish models for ant...
OBJECTIVE: To systematically identify traditional Chinese medicine (TCM) compound formulas with anti-atherosclerosis (AS) potential using a screening platform based on multi-strain gene knockout zebrafish models for anti-atherosclerosis TCM compounds. METHODS: Screening was conducted using a formula dictionary database, and compound formulas such as Dan Shen Fang, Shang Ni San, Jin Lingzi San, and Li Qi Hua Liang Tang were selected from 933 blood-activating and stasis-resolving formulas. Five strains of zebrafish-, , , , and -were fed a high-fat diet for 2 days, after which the AS model was established. DMSO (solvent control), rosuvastatin (western medicine positive control), compound Dan Shen Fang (TCM positive control), Shang Ni San, Jin Lingzi San, and Li Qi Hua Liang Tang were administered by bathing for 24 hours. Oil red O staining was used to detect lipid content, oxidative stress indicators, blood flow velocity, vascular endothelial cell damage, and the expression of inflammatory factors in zebrafish. The behavior patterns of inflammatory cells in zebrafish were observed, and the anti-AS efficacy of the Li Qi Hua Liang formulas was comprehensively evaluated. RESULTS: Compared with the AS model group, compound Dan Shen Fang and Shang Ni San improved lipid accumulation in the vascular lumen, reduced total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) levels, decreased oxidative stress response, inhibited the recruitment of neutrophils and macrophages, repaired vascular endothelial damage, improved blood flow velocity, and reduced the expression of inflammatory factors, thereby alleviating AS, with statistically significant differences. Jin Lingzi San and Li Qi Hua Liang Tang had limited effects on AS. CONCLUSION: This platform provides an efficient and targeted tool for the screening and research evaluation of anti-atherosclerosis TCM compound formulas, accelerates the research progress of anti-atherosclerosis TCM compound formulas, and lays a foundation for subsequent drug development and clinical application.
[Causal Multiple Mediation Analysis of the Association Between Early and Mid-Pregnancy Body Mass Index Trajectories and Preterm Birth].
Yang T, Cao Y, Zhou W … +2 more , Xiao C, Liu Z
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369708 · Full text
OBJECTIVE: To investigate the associations between body mass index (BMI) trajectories in early and mid-pregnancy and preterm birth, to examine the mediating effects of gestational diabetes mellitus (GDM) and inflammatory...
OBJECTIVE: To investigate the associations between body mass index (BMI) trajectories in early and mid-pregnancy and preterm birth, to examine the mediating effects of gestational diabetes mellitus (GDM) and inflammatory markers, and to provide evidence for early prevention and intervention of preterm birth. METHODS: A prospective cohort study was conducted and 1221 pregnant women who delivered babies at Chongqing Maternal and Child Health Hospital between 2018 to 2021 were included. Group-based trajectory modeling was used to identify BMI trajectories in early and mid-pregnancy. Logistic regression and causal mediation analyses were performed to evaluate the mediating effects of GDM and inflammatory factors in the associations between BMI trajectories and preterm birth. RESULTS: TThree BMI trajectories were identified, and the participants were categorized accordingly into Group 1 (48.98%), Group 2 (38.41%), and Group 3 (12.61%). Group 2 demonstrated a trajectory similar in shape to that of Group 1, with an overall level between those of Group 1 and Group 3. Participants in Group 3 were in the obese range in early pregnancy, with their BMI decreasing slightly along with the progression of pregnancy and stabilizing and then rising slightly in mid-pregnancy. After adjusting for confounders, Group 3 showed higher risks of preterm birth, which were 3.1 times those of Group 1 (odds ratio [OR] = 3.10, 95% CI: 1.59-6.06). Additionally, white blood cell count and monocyte percentage significantly mediated this association, with proportion eliminated of 0.5% and 5.1%, respectively. No mediating effect of GDM was observed. CONCLUSION: Pregnant women with higher BMI during early and mid-pregnancy exhibit a significantly increased risk of preterm birth. White blood cell count and monocyte percentage may serve as key inflammatory mediators linking this association while the mediating effect of GDM is not found.
[Mid- to Long-Term Urinary Continence Outcomes of Pelvic-Peritoneal Structure-Sparing Techniquein Robot-Assisted Radical Prostatectomy].
Yang L, Tu X, Lu G … +4 more , Dong Z, Bao Y, Liu Z, Wei Q
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369707 · Full text
OBJECTIVE: To evaluate the mid- to long-term effects of the pelvic-peritoneal structure-sparing (PPSS) technique, which aims to preserve the stability of pelvic floor structures, on urinary continence recovery after robo...
OBJECTIVE: To evaluate the mid- to long-term effects of the pelvic-peritoneal structure-sparing (PPSS) technique, which aims to preserve the stability of pelvic floor structures, on urinary continence recovery after robot-assisted radical prostatectomy (RARP). METHODS: This single-center retrospective cohort study included 82 patients who underwent RARP between October 2017 and May 2018, all operated on by the same surgeon. Patients were divided into a PPSS group ( = 53) and a non-PPSS group ( = 29). Standard RARP was performed in the non-PPSS group, whereas the PPSS group involved preservation of pelvic floor structures and cold-knife apical dissection for urethral function protection. Baseline characteristics were comparable between the two groups. Follow-up lasted up to 7 years. Urinary continence was defined as the use of 0-1 pad per day and was assessed at 3, 6, 12, 24 months postoperatively and at the last follow-up (July 2025). Kaplan-Meier analysis and Cox regression were used to compare continence recovery rates and identify influencing factors. RESULTS: The median follow-up duration was 1178 days (range: 1-2743 days). The PPSS group had significantly higher continence recovery rates at 3 and 6 months (71.6% and 75.4%) compared with the non-PPSS group (44.8% and 48.2%; = 0.030 and = 0.025, respectively), while no significant difference was observed at 12 months and thereafter. Multivariate Cox regression analysis showed that the PPSS technique (hazard ratio [HR] = 2.59, 95% CI: 1.39-4.81, = 0.002) and age (HR = 0.94, 95% CI: 0.91-0.97, = 0.001) were independent predictors of continence recovery. CONCLUSION: The PPSS technique improves early postoperative urinary continence without increasing the rate of positive surgical margins. Although it does not significantly enhance long-term continence, it may still contribute positively to patients' postoperative quality of life.
[Integrating Bioinformatics and Machine Learning Algorithms to Screen Inflammatory Biomarkers for Atrial Fibrillation and Experimental Validation].
Bu Q, Zhang W, Huang Y … +7 more , Li Z, Wang Y, Zhao X, Jia Y, Fan X, Yang Y, Wang Z
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369706 · Full text
OBJECTIVE: To identify potential biomarkers of atrial fibrillation (AF) using bioinformatics, machine learning (ML) and experimental methods. METHODS: AF transcriptomic data were obtained from the GEO and MSigDB database...
OBJECTIVE: To identify potential biomarkers of atrial fibrillation (AF) using bioinformatics, machine learning (ML) and experimental methods. METHODS: AF transcriptomic data were obtained from the GEO and MSigDB databases to screen for differentially expressed genes (DEGs) and inflammation-related gene sets (IRGs). The overlap between DEGs and IRGs was used to identify the DE-IRGs set. Two machine learning algorithms were used to filter AF-related DE-IRGs. Twelve male SD rats were randomly divided into a control group and an AF group. Rats received acetylcholine (66 μg/mL) and calcium chloride (10 mg/kg) via tail vein injection for five consecutive weeks to establish the AF model. Morphological characteristics of atrial myocytes were assessed with HE staining, while RT-qPCR and immunohistochemistry (IHC) were used to measure changes in mRNA and protein levels. RESULTS: In the training set, 119 DEGs were identified, with IRGs showing the highest correlation with two co-expression modules ( = 0.60 and 0.56, < 0.0001). The intersection of DEGs and IRGs yielded nine DE-IRGs. The SVM-RFE and RF algorithms identified 5-hydroxytryptamine receptor 2B gene (), latent-transforming growth factor beta-binding protein 2 gene (), matrix remodeling associated protein 5 gene ( ), and transforming growth factor β-induced protein gene () as highly expressed in both the training and test sets in AF groups with high diagnostic efficacy (AUC > 0.77). The electrocardiogram limb leads of AF rats showed numerous f-waves. HE staining revealed disorganized atrial myocyte arrangement and inflammatory cell infiltration in the AF group. The TUNEL fluorescence assay showed an apoptosis rate of (55.34 ± 4.29)% in the AF group, compared to (8.69 ± 3.12)% in the control group ( = 0.0001). There were statistically significant differences in the relative expression levels of and between the AF group (4.97 ± 4.20, 2.62 ± 1.85) and the control group (1.12 ± 0.21, 1.18 ± 0.77) ( = 0.0137, = 0.0444). IHC revealed positive rates of and proteins in the AF group of (36.50 ± 1.31)% and (27.39 ± 4.57)%, respectively, compared to (22.95 ± 2.62)% and (18.26 ± 3.70)% in the control group ( = 0.0008, = 0.0485). CONCLUSION: The characteristic genes and are highly expressed in AF and serve as valuable diagnostic biomarkers.
[The Role of Cuproptosis Related Key Genes in Ovarian Cancer and the Construction of a Prognostic Model].
Wang H, Jiang Y, Chen J … +2 more , Mu P, Zhang J
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369705 · Full text
OBJECTIVE: Using ovarian cancer datasets from public databases, identify copper death-related genes in ovarian cancer tissues and construct a clinical prognostic risk scoring model for ovarian cancer patients based on th...
OBJECTIVE: Using ovarian cancer datasets from public databases, identify copper death-related genes in ovarian cancer tissues and construct a clinical prognostic risk scoring model for ovarian cancer patients based on these genes. METHODS: We downloaded the OC data of TCGA, the GSE26193, GSE63885 dataset from GEO and retrieved 10 cuproptosis related genes (CRGs) and analyzed their chromosomal localization, expression correlation, and mutation patterns based on the datasets. Using these genes, we clustered the OC samples to identify different molecular subtypes of copper induced death. We analyzed the differential genes and functional enrichment between different subtypes and obtained feature genes with predictive ability for prognosis through survival regression analyses. Based on these feature genes, we constructed a risk scoring model and incorporated the clinical characteristics ofpatients to jointly predict their survival rate. RESULTS: In ovarian cancer samples, 10 copper death-related genes can stably divide the samples into two molecular subtypes, and there are significant differences in clinical and immune characteristics and drug sensitivity between them. After further screening, seven prognostic genes (、、、、、、) were obtained, and the risk model based on them combined with age predicted that the AUC of patients' 1-, 3-, and 5-year survival rates were all greater than 0.7, showing good clinical application prospects. CONCLUSION: The mechanism of cuproptosis and its key genes might become therapeutic targets for ovarian cancer. The subtypes of cuproptosis provide a theoretical basis for personalized clinical treatment. The predictive model constructed by key prognostic genes has promising clinical application effects.
[Effectiveness Analysis of Cervical Rotation-Traction Manipulation on Cervical and Dorsal Muscle Tone in Patients With Cervical Spondylotic Radiculopathy].
Wang X, Feng T, Zhu Y … +7 more , Zhao X, Sun K, Liu N, Xie R, Zhu L, Liu G, Wei X
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369704 · Full text
OBJECTIVE: To clarify the mechanism of action of the Cervical Rotation-Traction Manipulation in treating cervical spondylotic radiculopathy using shear wave elastography. METHODS: Thirty patients with cervical spondyloti...
OBJECTIVE: To clarify the mechanism of action of the Cervical Rotation-Traction Manipulation in treating cervical spondylotic radiculopathy using shear wave elastography. METHODS: Thirty patients with cervical spondylotic radiculopathy and 30 volunteers without abnormal cervical conditions in the past three months were included. Patients received manipulation therapy once a week for 15 to 20 minutes, totaling three sessions over two weeks. Volunteers received no treatment. Shear wave elastography was the primary research method (with Young's modulus as the primary outcome measure) to assess the effect of the Cervical Rotation-Traction Manipulation on the neck and back soft tissues in patients with cervical spondylotic radiculopathy. Shear wave elastography was performed on patients three times: before treatment, after the first manipulation session, and after two weeks of treatment. Volunteers underwent one shear wave elastography test as a baseline control. Secondary outcome measures were the visual analogue scale (VAS) and neck disability index (NDI) scores of patients before treatment, after treatment, and two weeks after treatment. RESULTS: The Young's modulus values of the cervical muscles in patients before treatment were higher than those in volunteers ( < 0.001), but there was no statistically significant difference after the treatment course. There were statistically significant differences in the Young's modulus of the muscle before and after treatment and before and after the treatment course in patients ( < 0.001). The Young's modulus values of the affected side cervical muscles were higher than those of the healthy side ( = 0.001), but there was no statistically significant difference after treatment. The median Young's modulus was positively correlated with the VAS score ( = 0.741, < 0.001) and the NDI score ( = 0.591, < 0.001) in patients with cervical spondylotic radiculopathy. CONCLUSION: The Cervical Rotation-Traction Manipulation can reduce the VAS and NDI scores of patients with cervical spondylotic radiculopathy, lower neck muscle tension, and balance the distribution of cervical muscle tension while reducing it. Young's modulus is a potential indicator reflecting the therapeutic effect of the spinal manipulation.
[An Improved Faster R-CNN Method for Wound Detection].
Zhang S, Shi Z, Jiang F … +3 more , Ding M, Liu C, Duan F
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369703 · Full text
OBJECTIVE: By introducing an attention enhancement mechanism and improving the post-processing strategy, the Faster Region-based Convolutional Neural Network (Faster R-CNN) is enhanced. The resulting improved Faster R-CN...
OBJECTIVE: By introducing an attention enhancement mechanism and improving the post-processing strategy, the Faster Region-based Convolutional Neural Network (Faster R-CNN) is enhanced. The resulting improved Faster R-CNN method for wound detection (WD-IFRCNN) increases the accuracy and stability of wound detection. METHODS: ① Algorithm construction: The 50-layer residual network (ResNet-50) is used as the backbone. In the fourth residual stage (convolutional stage 4, Conv4_x) and fifth residual stage (convolutional stage 5, Conv5_x), the fuzzy mask attention module (FMAM) and convolutional block attention module (CBAM) are embedded together to enhance the model's ability to represent features in key areas and blurry regions of the wound surface. At the same time, soft non-maximum suppression (Soft-NMS) replaces the traditional non-maximum suppression strategy to reduce missed detections in overlapping target scenarios. ② Algorithm validation: The experimental dataset consists of open-source wound images and clinically collected images, totaling 740 original images. After data augmentation, the dataset expands to 5920 images and is evaluated using ten-fold cross-validation. Model performance is assessed through internal validation, external validation, hyperparameter tuning, and ablation experiments, using metrics such as precision, recall, average precision, and F1 score. RESULTS: ① Internal validation showed that the model performs best when CBAM is embedded in both the Conv4_x and Conv5_x stages of ResNet-50. When ResNet-50-CBAM is used as the backbone, the model's detection performance surpasses that of VGG16 and ResNet-50. ② External validation showed that WD-IFRCNN achieves an precision of 92.31%, recall of 93.95%, average precision of 92.33%, and F1 score of 0.93. The average precision was 3.21%, 2.30%, 1.39%, 0.86%, 0.82%, 0.37%, and 0.63% higher than SSD, YOLOv4, YOLOv5, YOLOv8, DETR, RT-DETR, and FR-CNN-FPN, respectively. ③ Ablation experiments showed that CBAM, FMAM, and Soft-NMS each positively impact model performance, with the best results achieved when all are used together. CONCLUSION: WD-IFRCNN effectively enhances the model's ability to represent features in key areas and blurry regions of the wound surface, improves the accuracy and stability of wound detection, and demonstrates good adaptability to complex wound scenarios. It can provide technical support for clinical wound assessment and auxiliary diagnosis.
[Establishment of an Animal Model of High-altitude Polycythemia Under Natural Plateau Environment].
Nizhen, Fang L, Danzengdeqiong … +5 more , Wang J, Yangla, Duojizhuoma, Deyang, Basangzhuoma
Sichuan Da Xue Xue Bao Yi Xue Ban · 2026 May · PMID 42369702 · Full text
OBJECTIVE: To explore the time course and incidence of establishing an animal model of high-altitude polycythemia (HAPC) under a natural plateau environment (Lhasa, Xizang, 3 650 m above sea level) and to provide experim...
OBJECTIVE: To explore the time course and incidence of establishing an animal model of high-altitude polycythemia (HAPC) under a natural plateau environment (Lhasa, Xizang, 3 650 m above sea level) and to provide experimental technical support for high-altitude disease research. METHODS: Forty SPF grade Sprague-Dawley rats (20 males and 20 females) purchased from a low-altitude area (Chengdu, Sichuan, average altitude 500 m) were transported to Lhasa (3 650 m) and conventionally reared. A hematology analyzer (Sysmex XS-500i) was used to measure blood parameters daily during the first week after arrival and weekly thereafter; body mass was monitored daily with a precision electronic scale. Model success was determined according to the "Qinghai Criteria" (hemoglobin ≥ 190 g/L in females, ≥ 210 g/L in males). RESULTS: After 40 days of conventional rearing under natural plateau conditions (animal room temperature 17-25 °C, relative humidity 40%-70%), the mean hemoglobin (Hb) level reached 202 g/L (SE = 2.55) in females and 217 g/L (SE = 2.291) in males, indicating successful modeling. The success rate was higher in females (77%, 14/18) than in males (42%, 8/19). Successfully modeled rats exhibited behavioral abnormalities such as cold aversion, preference for warmth, reduced responsiveness, curled-up posture with less movement, ruffled and lusterless fur, and purplish-dark discoloration of the skin and mucous membranes of the lips, tongue, paws, and tail. Compared with plain controls, Hb, red blood cell count (RBC), hematocrit (HCT), red blood cell distribution width (RDW), mean corpuscular volume (MCV), and lymphocyte percentage (LYMPH%) showed statistically significant differences after entering the plateau ( < 0.05). Body weight gain was lower in females than in males ( < 0.001). The increase in Hb concentration was consistent with the upward trend of blood pressure, but the difference was not statistically significant ( > 0.05). CONCLUSION: Conventional rearing for 40 days under natural plateau conditions at 3 650 m can successfully establish an HAPC animal model in SD rats, with a higher success rate in females than in males.
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