Recent Patents On Biotechnology[JOURNAL]
Sun
200 papers
Konjac Gum in the Development of Hydrogel for Wound Dressing: A Bibliography and Patent Profile Review.
do Carmo VN, Dos Reis RMC, Cruz Santos CLD … +1 more , Pereira NP
Recent Pat Biotechnol · 2026 Jun · PMID 42304922 · Publisher ↗
Konjac gum is derived from the tuber Amorphophallus konjac K. Koch and is rich in Glucomannan, a polysaccharide with high gelling power. This study aims to evaluate, through a bibliographic and patent survey, the use of...
Konjac gum is derived from the tuber Amorphophallus konjac K. Koch and is rich in Glucomannan, a polysaccharide with high gelling power. This study aims to evaluate, through a bibliographic and patent survey, the use of Konjac gum in the production of pharmaceutical hydrogels. The research was carried out through databases such as Scopus, Web of Science, PubMed, and Medline, and by the Orbit Intelligence platform from 2004 to February 2025. In total, 12 articles and 25 patent families were identified, including patents related to the use of the Konjac hydrogel formulation range. 32% of the hydrogel formulations used on skin injuries analyzed had konjac in their composition due to its gelling action, and in 24% of patents, researchers introduced konjac gum in the development because of its healing potential. A 0.05 g concentration of konjac gum was the most common method for preparing antibacterial gels, which exhibited good biocompatibility and possessed excellent antibacterial and hemostatic properties, potentially aiding in the healing of infected wounds. Inventions using 1 g of glucomannan aimed to improve the hydrogel structure. Konjac, due to its greater biocompatibility, biodegradability, and renewability, in addition to its high gelling, healing, and inflammatory process reduction power, has aroused growing interest in the production of biomedical materials such as hydrogels for the treatment of skin wounds.
Design of an Optical Genosensor Based on Gold Nanoparticles for the Diagnosis of Breast Cancer Using miR-122.
Farzin Y, Atabi F, Movahedpour A … +1 more , Morowvat MH
Recent Pat Biotechnol · 2026 May · PMID 42163611 · Publisher ↗
INTRODUCTION: Early and accurate detection of breast cancer remains a major clinical challenge. In this study, we developed a rapid, optical genosensor based on gold nanoparticles for the detection of miR-122, a microRNA...
INTRODUCTION: Early and accurate detection of breast cancer remains a major clinical challenge. In this study, we developed a rapid, optical genosensor based on gold nanoparticles for the detection of miR-122, a microRNA associated with breast cancer. The biosensor design relies on acid-triggered aggregation of functionalized AuNPs, enabling colorimetric detection without amplification. METHODS: The R-122 oligonucleotide probe was designed using bioinformatics software. The designed genosensor was developed based on previously reported patented nanoplasmonic principles, employing gold nanoparticles for amplification-free optical detection of miR-122. Gold nanoparticles were synthesized via the citrate reduction method to obtain a uniform colloidal solution. RESULTS: The optical sensor exhibited a distinct absorption peak at 520 nm. Clinical serum samples containing complementary and non-complementary sequences produced clearly distinguishable color changes upon hybridization, confirming specific detection of the target sequence. Optimal visual discrimination was achieved by mixing 15 μL of nanobiosensor, 5.2 μL of acid, and 10 μL of PBS. DISCUSSION: The results demonstrate that the developed colorimetric AuNP-based genosensor provides an ultra-low detection limit and simple operation, showing promise for rapid clinical screening compared with previously reported SERS and electrochemical assays. CONCLUSION: The proposed AuNP-based colorimetric genosensor enables rapid and specific detection of miR-122 without amplification. Its simplicity, cost-effectiveness, and reliable visual output indicate strong potential for future point-of-care breast cancer diagnostics.
Adropin and Irisin as Potential Patentable Biomarkers in Metabolic-Associated Fatty Liver Disease: Current Evidence and Future Directions.
Roshni D, Saleem Z, Kamplikar P … +4 more , Ray SK, Kotnis A, Kanwar JR, Mukherjee S
Recent Pat Biotechnol · 2026 May · PMID 42152274 · Publisher ↗
Metabolic-associated fatty liver disease (MAFLD), formerly nonalcoholic fatty liver disease (NAFLD), is the most common long-term liver disease. It affects 25% of adults and is a major cause of liver disease worldwide. I...
Metabolic-associated fatty liver disease (MAFLD), formerly nonalcoholic fatty liver disease (NAFLD), is the most common long-term liver disease. It affects 25% of adults and is a major cause of liver disease worldwide. Its rise is linked to rising obesity, especially in Western nations. Unfortunately, no MAFLD medication works. Patents and intellectual property are essential to developing effective cures and protecting new ideas. Long-term aerobic activity can raise hepatic triglycerides and lower visceral fat without weight loss. Due to its complexity, MAFLD is difficult to diagnose and treat. There are no inexpensive, reliable imaging diagnostics or non-invasive biomarkers. Too many liver lipids can cause lipotoxicity. This can cause NASH, liver fibrosis, and hepatocellular cancer. More serious MAFLD and NASH accelerate necroinflammation and fibrosis. This shows how important good signals are for diagnosing and treating your ailment. Invasive liver biopsies aren't helpful since people don't handle them well. We need reliable non-invasive assays to detect MAFLD early, improve clinical trials, and confirm therapy. Irisin, a hormone that boosts energy expenditure and mimics brown fat, is elevated in physically active people. Patenting discoveries about it could speed up research and the development of tailored treatments. Patenting innovative biomarker research concepts and intellectual property is essential to combating MAFLD. This will also enable novel illness diagnosis and treatment methods. Our approach must involve new MAFLD diagnosis and treatment methods and patent issues to address all its effects.
Anticancer Efficacy of Leaf Extract in Ehrlich's Carcinoma-Bearing BALB/c Mice.
Semwal S, Kumar N, Joshi BC … +1 more , Verma P
Recent Pat Biotechnol · 2026 · PMID 41992462 · Publisher ↗
BACKGROUND: The plant Cinnamomum tamala (Buch.-Ham.) T. Nees & C.H. Ebern. (Family: Lauraceae) is commonly known as 'tejpaat' in India, has leaves and bark reported to possess anticancer, immunomodulatory, antidiabetic,...
BACKGROUND: The plant Cinnamomum tamala (Buch.-Ham.) T. Nees & C.H. Ebern. (Family: Lauraceae) is commonly known as 'tejpaat' in India, has leaves and bark reported to possess anticancer, immunomodulatory, antidiabetic, and diuretic activities. The objective of the present study was to explore the antitumor potential of the chloroform extract of Cinnamomum tamala leaves (CTCE) in BALB/c mice against Ehrlich's Carcinoma (EC). METHODS: Based on preliminary in-vitro cytotoxicity studies, CTCE was selected for an in-vivo antitumor study. Anticancer activity of CTCE was evaluated in BALB/c mice against EC at the doses of 50, 100, 250, and 500 mg/kg body weight. CTCE was administered for 15 consecutive days after induction of the tumor. After 24 hours from the last administered dose and 18 hours of fasting, half of the mice were sacrificed, while the other half was kept alive to evaluate any potential for increasing the lifespan. The antitumor effect of CTCE was assessed by evaluating tumor volume, hematological parameters, and the life span of the EC-bearing host. RESULTS: CTCE showed a significant decrease (p<0.05) in tumor volume and increased the lifespan of EC tumor-bearing mice in a dose-dependent manner compared with the untreated group. The hematological profile, including RBC count, hemoglobin, WBC count, and DLC, was also improved upon treatment. DISCUSSION: C. tamala chloroform extract (CTCE) showed significant anticancer potential by reducing cell viability, inhibiting tumor growth, and prolonging survival without toxicity, suggesting it as a promising source of bioactive compounds for anticancer drug development. CONCLUSION: The results suggest that CTCE exhibits dose-dependent anticancer activity in comparison with EC control mice and demonstrates significant effects relative to doxorubicin. Its antitumor activity may be attributed to the presence of active constituents within the extract. This investigation also highlights recent advancements in intellectual property rights and patent strategies related to plant-derived anticancer agents.
Unveiling the Modern Therapeutic Properties and Folk Medicinal Riches of .
Sharma D, Jain D, Bhatia D
Recent Pat Biotechnol · 2026 · PMID 41992461 · Publisher ↗
Rubia cordifolia (Manjishtha), a perennial herb of the Rubiaceae family, has been valued in traditional medicine for its diverse pharmacological properties. Predominantly cultivated in hilly regions, its roots have been...
Rubia cordifolia (Manjishtha), a perennial herb of the Rubiaceae family, has been valued in traditional medicine for its diverse pharmacological properties. Predominantly cultivated in hilly regions, its roots have been historically used for their red pigment and therapeutic applications in Ayurveda. R. cordifolia has been traditionally employed for skin diseases, menstrual disorders, snake bites, herpes, eye diseases, haemorrhoids, and fractures. Modern research highlights its potent antioxidant, antimicrobial, and hepatoprotective properties, with evidence supporting its role in managing acne, inflammation, cancer, diabetes, Alzheimer's disease, and infectious diseases. Furthermore, its integration into various Ayurvedic formulations emphasizes its clinical significance. A comprehensive literature review highlights its bioactive compounds and their therapeutic relevance in modern medicine. The review aims to provide a comprehensive knowledge of the pharmacological impacts, the active components, and the medicinal applications of R. cordifolia. It uncovers new bioactive compounds, mechanisms of action, or novel formulations, patent protection becomes essential.
Advancements in Pharmacotherapy and Mobile Health Applications for Self-Management in Multiple Sclerosis: A Comprehensive Review.
Heidaryfar S, Poursadeghfard M, Morowvat MH
Recent Pat Biotechnol · 2026 Mar · PMID 41863501 · Publisher ↗
INTRODUCTION: Multiple sclerosis (MS) is a chronic autoimmune demyelinating disorder of the central nervous system, affecting nearly three million individuals worldwide. Long-term pharmacotherapy and treatment adherence...
INTRODUCTION: Multiple sclerosis (MS) is a chronic autoimmune demyelinating disorder of the central nervous system, affecting nearly three million individuals worldwide. Long-term pharmacotherapy and treatment adherence remain major determinants of relapse prevention and disability progression. METHODS: Following PRISMA guidelines, a narrative review compared FDA and EMA approved disease-modifying therapies (DMTs) for MS. Clinical, real-world, and patentbased data were synthesized to evaluate efficacy, adherence, and safety profiles. Digital health applications supporting self-management were also assessed. RESULTS: Injectable therapies such as interferons and glatiramer acetate showed approximately 30% ARR reduction with excellent safety. Oral agents (fingolimod, dimethyl fumarate, teriflunomide, cladribine) achieved 45-55% ARR reduction and markedly better adherence, though periodic laboratory monitoring was required. Monoclonal antibodies (natalizumab, ocrelizumab) reached up to 68% relapse reduction, representing the highest efficacy while demanding close surveillance for PML and infection risks. DISCUSSION: While newer high-efficacy agents yield stronger disease control, the balance between potency, adherence, and practicality remains crucial. Oral DMTs provide the most realistic compromise for long-term management. Digital self-monitoring through validated m-health applications could substantially improve adherence and disease awareness. Integration of biotechnology with tele-neurology and AI-supported analytics represents the next step toward personalized MS care. CONCLUSION: Monoclonal antibodies define the upper limit of efficacy in current MS therapy, but sustainability depends on safety oversight and patient engagement. Oral formulations remain clinically pragmatic first-line options. The synergy between pharmacotherapy and mobile health technology offers a pathway to transform adherence, monitoring, and outcome optimization in future MS management.
The Diagnostic and Prognostic Potential of Serum Taurine and PUMA in Staging Colorectal Cancer Patients.
Elashry MA, Samir S, Elrefaiy M … +4 more , Hammam O, Abu-Taleb H, Ossama Y, Elmeligy HA
Recent Pat Biotechnol · 2026 Feb · PMID 41764612 · Publisher ↗
INTRODUCTION: Ranked as the third most diagnosed cancer globally, colorectal cancer (CRC) is the seventh most prevalent cancer in Egypt. This study aimed to assess the levels of taurine (Tau), carcinoembryonic antigen (C...
INTRODUCTION: Ranked as the third most diagnosed cancer globally, colorectal cancer (CRC) is the seventh most prevalent cancer in Egypt. This study aimed to assess the levels of taurine (Tau), carcinoembryonic antigen (CEA), and p53 upregulated modulator of apoptosis (PUMA) in CRC patients. METHODS: We conducted a pilot study involving 70 patients with CRC. Serum Tau was measured using ELISA, CEA was evaluated by immunohistochemistry (IHC), and PUMA expression was assessed using quantitative real-time PCR (qPCR). All measurements were taken before surgery, one week after surgery, and one month later. RESULTS: Tau levels were significantly lower in adenocarcinoma groups (G1, G2, and G3) and in the malignant metastatic group compared with the control and inflammatory groups (p < 0.001). Tau levels increased in G1, G2, and G3 patients one week and one month after surgery. PUMA expression after surgery was higher than before surgery in G1, G2, and G3 adenocarcinoma groups, while it was absent in the metastatic group. The specificity of the Tau test before surgery, one week after, and one month after was 53.33%, 76.92%, and 46.67%, respectively. The specificity of the PUMA test was 76.92% before surgery and 71.43% after surgery. CEA levels were significantly higher in adenocarcinoma (G1 and G2) compared with control and inflammatory groups, whereas CEA staining intensity was significantly decreased in adenocarcinoma (G1 and G2) compared with these groups (p < 0.001). DISCUSSION: PUMA expression tends to be lower in advanced CRC stages and increases after surgery, supporting its potential role in tumor suppression and prognosis. Taurine shows variable levels among CRC patients and may aid in non-invasive cancer staging; its lower serum levels are associated with more advanced disease, and levels rise following surgical removal of the tumor. CONCLUSION: This study highlights PUMA as a potential prognostic indicator for CRC. Tau levels may also hold diagnostic value and could contribute to the development of non-invasive screening methods for early CRC detection, particularly within the Egyptian population, which remains underrepresented in current research.
Halo-tolerant Plant Growth Promoting Rhizobacteria Enhancing Mustard Seed Growth Under Salinity Stress for Sustainable Agriculture.
Sharma K, Sharma PK, Prasad SR … +3 more , Singh A, Jain D, Singh S
Recent Pat Biotechnol · 2026 · PMID 41714949 · Publisher ↗
INTRODUCTION: Salinity is one of the primary environmental factors that significantly impact global crop production. Plant growth promoting rhizobacteria (PGPR) inoculation to crops improves the productivity of the crops...
INTRODUCTION: Salinity is one of the primary environmental factors that significantly impact global crop production. Plant growth promoting rhizobacteria (PGPR) inoculation to crops improves the productivity of the crops. METHODS: To develop a biofertilizer specifically for saline soil, bacteria were isolated from the rhizosphere of mustard plants along with the plant growth-promoting traits grown in saline soil (EC 6 dS m). Halotolerant 22 bacterial strains were isolated and identified from the rhizospheric soil mustard crop, Purvanchal (Indian state). According to the study, 54.54% of the isolates had phosphate solubilization efficiencies ranging from 7% to 27% on plate assays. According to quantitative measurements, 63.63% of the strains exhibited the ability to solubilize phosphate, with degrees of solubilization varying between 0.49 and 3.34 μg/ml. Furthermore, 50% of the isolates showed the ability to solubilize zinc, with solubilization rates varying from 12% to 53%. Further 59.09% of the bacterial strains showed ammonium production test; these strains were classified as having low (+), medium (++), and high (+++) levels of ammonium production. RESULTS: According to the research, these halo-tolerant plant growth-promoting rhizobacteria (PGPR) have particular functional properties that may help mustard crops grow more rapidly in salinity-stressed environments. Because these PGPR strains increase nutrient availability and stimulate plant development, they may find use in agriculture, especially in saline settings. CONCLUSION: The study emphasizes how crucial it is to use PGPR with particular nutrient mobilization features to promote crop growth under difficult circumstances. The identification of these efficient strains may lead to the development of patent biofertilizers designed for saline soils, further supporting their application in modern agricultural practices.
A Review on the Production of Cost-Effective Biodiesel from Animal Fat Wastes.
Aslam S, Khalil Y, Butt FR … +6 more , Nawaz Y, Munir S, Riaz HF, Azam S, Parveen M, Zaib S
Recent Pat Biotechnol · 2026 Jan · PMID 41588769 · Publisher ↗
INTRODUCTION: Biodiesel from animal waste provides an alternative source of fuel. It is eco-friendly and cheaper than conventional fuel obtained through the distillation of crude oil. Biodiesel is similar to petroleum di...
INTRODUCTION: Biodiesel from animal waste provides an alternative source of fuel. It is eco-friendly and cheaper than conventional fuel obtained through the distillation of crude oil. Biodiesel is similar to petroleum diesel and can be used alone or blended with fossil diesel as an energy source. This study provides insight into the use of waste animal fats for biodiesel production. METHODS: The data were retrieved from different sources, including PubMed, Google Scholar, and other scientific websites. Scope of the study can be clearly understood from the facts revealed from patent work. RESULTS: The use of waste animal fats provides a renewable and eco-friendly alternative to petroleum diesel. Both homogeneous and heterogeneous catalysis methods have proven effective for processing WAFs, with KOH and NaOH commonly used in concentrations ranging from 1% to 2%. Increasing the catalyst concentration within a certain range can enhance biodiesel yield. However, the use of acid catalysts for transesterification of WAFs is time-intensive and requires a high alcohol-to-fat ratio. Despite these advantages, challenges remain in catalyst reuse for certain reactions and in managing complex selectivity combinations. Lipases in water-poor environments are also employed for transesterification. DISCUSSION: The fatty acid profiles of both animal and vegetable sources have been found suitable for biodiesel production. Although it is a cheap source for generating fuel, using specific reaction media such as acyl acceptors and employing a combination of two enzymes on a specialized support can help further reduce costs. CONCLUSION: Currently, biodiesel is widely used in developed countries such as the US and Germany, but its adoption remains limited in developing nations. Optimizing transesterification methods-including the use of heterogeneous or homogeneous catalysis- and incorporating innovative technologies such as microwave-assisted enzymes can further enhance biodiesel yield and efficiency.
Deciphering the Regulatory Landscape of mRNA-miRNA-lncRNA Interactions in COVID-19 Severity: Insights from Transcriptomic Analysis.
Sabetian S, Sadeghi M, Ahmadi ES … +3 more , Cava C, Dara M, Morowvat MH
Recent Pat Biotechnol · 2026 Jan · PMID 41588768 · Publisher ↗
BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, has a highly variable clinical course, ranging from mild symptoms to severe systemic complications. Understanding...
BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, has a highly variable clinical course, ranging from mild symptoms to severe systemic complications. Understanding the molecular basis of disease severity is essential for improving diagnostic and therapeutic strategies. OBJECTIVE: In this study, we performed transcriptome analysis of peripheral blood mononuclear cells (PBMCs) from patients with mild and severe COVID-19 to identify differentially expressed mRNAs and long noncoding RNAs (lncRNAs), and to construct a regulatory network including microRNAs (miRNAs). METHODS: Transcriptome analysis of peripheral blood mononuclear cells (PBMCs) from patients with mild and severe COVID-19 was performed to identify differentially expressed mRNAs and long noncoding RNAs (lncRNAs), and to construct a regulatory network including microRNAs (miRNAs) using Cytoscape. RESULTS: A total of 674 mRNAs and 215 lncRNAs were differentially expressed between severe and mild cases, with key pathways enriched in cytokine-cytokine receptor interaction and immunological synapse signaling. The lncRNAs NEAT1 and MALAT1 were identified as regulatory hubs and were broadly expressed across multiple immune and organ tissues. Predicted miRNAs (experimentally supported in interaction databases) were associated with inflammation- and cancer-related signaling pathways, including PI3K-Akt, JAK/STAT, and TNF. DISCUSSION: Our findings are consistent with recent patents (e.g., US2022/0298584A1, EP3892280A3, WO2023147669A1) that propose the use of noncoding RNAs and cytokines as biomarkers for COVID-19 diagnosis and severity assessment. CONCLUSION: Our findings are consistent with recent patents (e.g., "US20220298584A1", "EP3892280A3", "WO2023147669A1") that propose the use of noncoding RNAs and cytokines as biomarkers for COVID-19 diagnosis and severity assessment. This integrative transcriptomic analysis highlights the regulatory role of noncoding RNAs in COVID-19 progression, exemplified by the central hub lncRNAs NEAT1 and MALAT1, which interact with inflammation- associated miRNAs and mRNA targets to modulate cytokine signaling. These findings offer specific transcriptomic biomarkers with potential for clinical application and therapeutic targeting.
Patented Fibrinolytic Enzymes: An Overview.
Cao Y, Gou M, Zar A … +2 more , Shestakova A, Osmolovskiy A
Recent Pat Biotechnol · 2025 Oct · PMID 41121484 · Publisher ↗
Fibrinolytic enzymes have garnered significant attention from researchers due to their immense potential in the medical field. As research continues to advance, the outcomes have become increasingly fruitful. The generat...
Fibrinolytic enzymes have garnered significant attention from researchers due to their immense potential in the medical field. As research continues to advance, the outcomes have become increasingly fruitful. The generation of applicable knowledge is usually accompanied by its protection through patent applications. This article compiles all patents related to "fibrinolytic enzyme" from Google Patents and the European Patent Office's Espacenet database, analyzing their core information, including publication year, application country, patent status, source of the fibrinolytic enzyme, and its various biochemical features. By combining relevant patent protection with current literature research, this article provides a novel and forward-looking summary of the current research status in the field of fibrinolytic enzymes.
Evaluation of the Use of Different Oils and Emulsifiers in Oil-in-Water (O/W) Nanoemulsions.
da Cruz Monteiro Santana T, Moraes Dos Santos Fonseca L, Valéria Saraiva Hodel K … +4 more , de Abreu Barreto G, Devequi Gomes Nunes D, de Jesus SIlva M, Aparecida Souza Machado B
Recent Pat Biotechnol · 2025 Oct · PMID 41121483 · Publisher ↗
INTRODUCTION: Nanoemulsions (NEs) are a biphasic systems formed by two immiscible liquids: (1) oil-in-water or (2) water-in-oil emulsions. These systems exhibit unique physicochemical properties due to their small partic...
INTRODUCTION: Nanoemulsions (NEs) are a biphasic systems formed by two immiscible liquids: (1) oil-in-water or (2) water-in-oil emulsions. These systems exhibit unique physicochemical properties due to their small particle size, making them more versatile compared to other emulsion systems. Thus, this research aimed to produce and evaluate oil-in-water nanoemulsions. METHODS: NE's were prepared using Squalene, grape seed, and avocado oils, combined with soy lecithin or soy phosphatidylcholine as emulsifiers. The selection of oils considered both regulatory aspects and patent restrictions. Formulations were produced by microfluidization, and their physicochemical properties, such as particle size, polydispersity index, and zeta potential, were assessed at day one (D0) and day 7 (D7), under storage at 4°C, 25°C, and 40°C. RESULTS: The nanoemulsions produced with soy phosphatidylcholine and vegetable oils exhibited good preliminary stability, due to their small particle size. In contrast, formulations using soy lecithin as the emulsifier did not show favorable results, especially the one with grape seed oil, which showed a large particle diameter. However, none of these formulations exhibited cellular cytotoxicity. DISCUSSION: Emulsifier selection had a strong impact on NEs characteristics, with Soy- PC producing smaller and more uniform particles compared to soy lecithin. All NEs showed good biocompatibility in fibroblasts, indicating their safety. CONCLUSION: Among the formulations, the one with phosphatidylcholine demonstrated a safer and more reliable stability profile, making it a promising candidate for application in the pharmaceutical and cosmetics industries.
Bioinformatic Design of the Secreted form of SAG2 Protein as a Toxoplasma gondii Vaccine Candidate.
Mesri A, Asadi N, Maleki-Kakelar H … +1 more , Taheri-Anganeh M
Recent Pat Biotechnol · 2025 Oct · PMID 41102957 · Publisher ↗
INTRODUCTION: Toxoplasmosis is one of the most common parasitic infections transmitted between humans and animals, caused by the protozoan Toxoplasma gondii. This organism acts as an obligate intracellular parasite, pres...
INTRODUCTION: Toxoplasmosis is one of the most common parasitic infections transmitted between humans and animals, caused by the protozoan Toxoplasma gondii. This organism acts as an obligate intracellular parasite, presenting an active and rapidly proliferating form known as a tachyzoite, in addition to two durable forms: the tissue cyst and the oocyst. Due to the considerable global impact of chronic infections in various host species, especially in humans and domestic animals, creating and producing an effective vaccine for preventing and managing toxoplasmosis is critically essential. The excretory antigens of Toxoplasma gondii play a vital role in vaccine development and diagnostic processes, with the SAG2 antigen being particularly significant. At present, sophisticated bioinformatics tools are utilized to design efficient signal peptides. This research will adopt an in silico approach to evaluating different signal peptides. METHODS: The signal peptide sequence was obtained from the Signal Peptide website and Uniprot. Following this, SignalP was utilized to predict the signal peptides and determine the positions of their cleavage sites. The ProtProm tool was then employed to assess the physicochemical characteristics and solubility. Furthermore, ProtCompB was used to forecast the subcellular localization of the fusion protein. RESULTS: Of the 50 signal peptides analyzed, 49 were excluded based on SignalP criteria. The signal peptide with the highest aliphatic index was HDEA_ECOLI, while TORT_ECOLI exhibited the highest GRAVY value. DISCUSSION: Additionally, SAG2, when fused with all signal peptides, was explicitly designed for the Sec pathway, except NAPA_ECOLI, MBHT_ECOLI, CUEO_ECOLI, and OPGD_ECOLI. CONCLUSION: An appropriate signal peptide was recognized for the fusion and translocation of SAG2 into the extracellular environment. While additional experimental studies are necessary for conclusive validation.
Demand Forecasting in the Tunisian Pharmaceutical Industry: A Comparative Study.
Mrad M, Boujelbene Y
Recent Pat Biotechnol · 2025 Oct · PMID 41088901 · Publisher ↗
INTRODUCTION: Demand forecasting is critical for the pharmaceutical industry to ensure efficient production, inventory management, and distribution, especially in dynamic and competitive markets. This study addresses the...
INTRODUCTION: Demand forecasting is critical for the pharmaceutical industry to ensure efficient production, inventory management, and distribution, especially in dynamic and competitive markets. This study addresses the challenges of accurate demand prediction within the Tunisian pharmaceutical sector. METHODS: The primary aim was to compare the forecasting accuracy of two methods, Holt-Winters (HW) and Multilayer Perceptron (MLP) neural networks, for three drug categories: Antiviral, Antibiotic, and Pain Relief. Additionally, the study provides actionable recommendations to enhance forecasting strategies. A 24-month (n=24) historical sales dataset (October 2020 to September 2022) from a Tunisian pharmaceutical demand forecasting company was analyzed. The analysis utilized the Holt-Winters model to incorporate seasonal adjustments and an MLP neural network to capture complex, non-linear sales patterns. RESULTS: Both models were evaluated using metrics such as mean squared error (MSE) to quantify prediction accuracy. The MLP neural network consistently and significantly outperformed the Holt-Winters method, demonstrating markedly lower MSE values (e.g., 0.0206 for Antivirals, compared to 30.06 for HW) and greater adaptability to demand variability across all drug categories. DISCUSSION: While effective for seasonal patterns, HW struggled with irregular fluctuations and complex dynamics. This study highlights the superiority of MLP neural networks for pharmaceutical demand forecasting due to their adaptability and accuracy in handling non-linear and variable data. CONCLUSION: The findings provide a strong quantitative basis for Tunisian pharmaceutical companies to adopt advanced machine learning techniques for more reliable planning. However, to fully realize the potential of these techniques and address the limitations outlined in this research, future research should explore hybrid models and integrate extended datasets that incorporate external market dynamics and trends.
Investigating Molecular Biomarkers in E. coli-Induced Urinary Tract Infections: Insights into the Long Non-Coding RNAs Contribution to Antimicrobial Resistance.
Nasr R, Mohamed Nasr S, Okasha H … +2 more , Dahroug H, Ibrahim MK
Recent Pat Biotechnol · 2025 Oct · PMID 41084247 · Publisher ↗
INTRODUCTION: Urinary Tract Infections (UTIs) caused by Escherichia coli are a major concern due to rising antimicrobial resistance. Long non-coding RNAs (lncRNAs) play crucial roles in gene regulation, but their involve...
INTRODUCTION: Urinary Tract Infections (UTIs) caused by Escherichia coli are a major concern due to rising antimicrobial resistance. Long non-coding RNAs (lncRNAs) play crucial roles in gene regulation, but their involvement in UTIs and their implication in antimicrobial resistance mechanisms are not well understood. This study investigates the association between specific long non-coding RNAs, immune response, and antibiotic resistance in patients with infections caused by E. coli. METHODS: Quantification of specific lncRNAs (NEAT1-1, NEAT1-2, MIR3142HG, AK170409, and IL7AS) was performed using quantitative PCR. Inflammatory markers IL-1β, IRF3, and NF-κB were measured in UTI patients using ELISA kits to assess their biological response. The minimum inhibitory concentration of eleven antibiotics was determined by testing all 25 urine samples and categorized as sensitive, intermediate, or resistant. RESULTS: NEAT1-1, NEAT1-2, MIR3142HG, and AK170409 were significantly upregulated (p < 0.05). Inflammatory markers were significantly elevated in all samples: IL-1β at 72.36 ± 13.8 pg/mL, IRF3 at 79.36 ± 15.01 pg/mL, and NF-κB at 4.43 ± 0.82 pg/mL (p < 0.0001). NEAT1-2 and AK170409 expression correlated with distinct antibiotic response patterns (p < 0.05). Differences in biological and hematological data were observed among UTI patients with varying topographic expressions of specific long noncoding RNAs (lncRNAs). DISCUSSIONS: Specific lncRNAs may be involved in modulating immune responses and influencing antibiotic susceptibility in UTIs. Their expression patterns reflect both the severity of infection and resistance profiles, suggesting a functional role in the development of antimicrobial resistance. CONCLUSION: LncRNAs are potential biomarkers for UTIs caused by E. coli. Future studies should focus on elucidating the precise role of lncRNAs in the development of antibiotic resistance in these infections.
Exploring Novel Microorganisms for PAH Degradation in Egyptian Soil: A Bioremediation Strategy for Soil Detoxification.
Shendy AA, Abdelhakim HK, El-Desouky MA … +1 more , Rashidi FB
Recent Pat Biotechnol · 2025 Aug · PMID 40917028 · Publisher ↗
INTRODUCTION: Polycyclic aromatic hydrocarbons (PAHs) are toxic petroleum byproducts in soil, exhibiting significant genotoxic properties. Microorganisms residing in contaminated soils serve as effective detoxifying agen...
INTRODUCTION: Polycyclic aromatic hydrocarbons (PAHs) are toxic petroleum byproducts in soil, exhibiting significant genotoxic properties. Microorganisms residing in contaminated soils serve as effective detoxifying agents. Among various strategies, bioremediation is an efficient biological method for detoxifying PAHs. METHOD: Hundreds of soil samples were collected from the Gulf of Suez, Egypt. The isolation process utilized an enrichment culture system with phenol naphthalene (PN) (10 mg/mL) as the primary carbon source. HPLC analysis was applied to confirm PN degradation. Consequently, the bacterial strain was characterized morphologically, biochemically, and through partial sequencing of its 16S rRNA gene. Subsequently, its plasmid was purified to transfer its phenotype to Escherichia coli. Finally, a bioremediation approach was conducted to test its PAH degradation. RESULTS: HPLC analysis was performed to confirm PN degradation by the isolated strain. The isolated strain was identified as Lysinibacillus species AAS1 (OR044755.1) with 98.43% sequence similarity to the Lysinibacillus genus. Subsequently, E. coli transformants with the isolated plasmid were grown in the presence of PN as the primary carbon source. Finally, the bioremediation assay of the isolated strain exhibited a high efficiency in detoxifying PN. DISCUSSION: The novel identified Lysinibacillus species AAS1 (OR044755.1) shows promise for PAHs detoxification, which may lead to the exploration of a biological agent for the remediation of water and soil contaminated with PAHs. CONCLUSION: A novel bacterial strain bearing a plasmid that can degrade PN was isolated from Egyptian petroleum waste-contaminated soil. It paved the way for further studies to isolate the whole gene(s) responsible for such degradation.
Economic Assessment of Bio-oil Production from Algae: A Bibliometric Analysis.
Jaithaliya R, Jain D, Chakraborty D … +1 more , Dutt K
Recent Pat Biotechnol · 2025 Aug · PMID 40917027 · Publisher ↗
INTRODUCTION: The production of bio-oil from microalgae is gaining attention as an alternative renewable energy source. To generate advances in this field, it is essential to identify the gaps in existing research. Overc...
INTRODUCTION: The production of bio-oil from microalgae is gaining attention as an alternative renewable energy source. To generate advances in this field, it is essential to identify the gaps in existing research. Overcoming this barrier necessitates addressing methodologies that can assess the existing work and develop relationships between publications, research groups, and their impact. METHODS: To chart a course toward practicality, this study employs bibliometric indicators, leveraging tools such as R Studio, VOSviewer 1.6.19, and Biblioshiny for network visualization and analysis. Drawing on the Scopus database spanning 2004-2024, a meticulous keyword strategy effectively segregated relevant papers. RESULTS: The findings suggest that the total number of published documents is 1005. The publication trend on this topic shows a significant increase since 2008, with a record high of 107 publications in 2022. China dominates the publication list, with thirteen universities contributing the highest. In the context of algal bio-oil, a total of 2850 authors contributed to 1005 publications. DISCUSSION: A three-way map displaying the relationships between journal titles, paper titles, and author keywords provides useful insights into the research landscape of the field. Algal research is growing and collaborating, despite bibliometric limitations such as time dependency and database scope. Such studies, however, offer valuable insights into the evolving bio-oil field. CONCLUSION: The study aims to link sustainable economic development with technological innovation and related economic growth. Its objective is to provide researchers with information on niche research clusters, quantify emerging methodologies, facilitate collaborative networks, and identify knowledge gaps, while assisting in determining the impact of specific publication venues. In addition to analyzing key economic drivers, the study will also recommend prospective research themes to disseminate algae-based biooil production research.
Exploring the Multifaceted Health Benefits of Natural Dietary Sources: Antimicrobial, Antioxidant, Anticancer, and Antidiabetic Properties.
Kaushik P, Gahalayan A, Parashar S … +4 more , Rani M, Khurana N, Dogra S, Pandey P
Recent Pat Biotechnol · 2025 Aug · PMID 40820455 · Publisher ↗
The lifestyle of today's generation contributes to various health issues like cancer, diabetes, obesity, heart disease, and high blood pressure. A significant factor contributing to these harmful lifestyle choices is the...
The lifestyle of today's generation contributes to various health issues like cancer, diabetes, obesity, heart disease, and high blood pressure. A significant factor contributing to these harmful lifestyle choices is the overconsumption of highly processed, energy-dense foods that are rich in saturated and trans fats, sodium, and added sugars, Conversely, adopting healthier dietary patterns that prioritize the intake of fruits, vegetables, whole grains, lean proteins, and healthy fats has been shown to protect against these chronic health conditions. Investigating the diverse health benefits of natural food sources requires a holistic approach encompassing dietary intake evaluations, laboratory and animal studies, and human clinical research. These investigations examine the antimicrobial, antioxidant, cancer-fighting, and blood sugar-regulating properties of compounds derived from plants. Studies indicate that diets abundant in whole grains, fruits, and vegetables supply crucial nutrients and biologically active substances such as polyphenols and flavonoids, which provide protective benefits against long-term disease conditions, including heart disease, cancer, and diabetes. Consequently, it is imperative to adjust our dietary practices and lifestyle choices to mitigate the risk of various ailments. Naturally occurring compounds such as curcumin, quercetin, kaempferol, and resveratrol, which are found in diverse food sources, have the potential to combat numerous diseases when incorporated into our diets. This review explores an array of compounds present in dietary sources and their associated biomedical properties, including their anticancer, antidiabetic, antioxidant, and antimicrobial effects. Furthermore, it explores various dietrelated strategies designed to promote a healthier lifestyle, including the incorporation of a diverse range of fruits, vegetables, and spices rich in polyphenolic compounds into one's daily nutritional intake.
Antimicrobial Activity of Leaf and Root Parts of Cenchrus biflorus Roxb.
Chaudhary S, Bhuyan R, Jain D … +1 more , Prakash A
Recent Pat Biotechnol · 2025 Aug · PMID 40820454 · Publisher ↗
INTRODUCTION: Medicines and herbal formulations are derived from different parts of medicinal plants, which are the best-known sources for treating various diseases. This research focuses on assessing the antimicrobial p...
INTRODUCTION: Medicines and herbal formulations are derived from different parts of medicinal plants, which are the best-known sources for treating various diseases. This research focuses on assessing the antimicrobial potential of crude extracts from the leaves and roots of Cenchrus biflorus Roxb. METHODS: Methanol, hydroethanol (50:50), and aqueous extracts were obtained using the Soxhlet extraction method. The disc diffusion method was used to study the antimicrobial activity of the extracts against a variety of test microorganisms, including bacteria (Escherichia coli and Bacillus subtilis) and fungus (Aspergillus niger). The disc diffusion method was used to assess bacterial susceptibility, revealing the potent inhibitory effect of the methanol extract on E. coli. All extracts demonstrated significant antimicrobial activity against various microorganisms. RESULTS: Remarkably, methanol extract of leaf demonstrated the highest antibacterial activity, with a 16.3 ± 1.78 mm zone of inhibition (ZOI) with Activity Index (AI) of 0.875, and a Relative Percentage Inhibition (RPI) of 80 against E. coli, followed by Bacillus subtilis (ZOI = 15.5 ± 1.31 mm, AI = 0.869, RPI = 78.57). The methanol extract of the root showed strong antifungal activity against Aspergillus niger (with a 12.9 ±1 mm ZOI, AI = 0.636, and RPI = 42.85), while the water extract of the root displayed 7.8 mm inhibition zones. DISCUSSION: Methanol and hydroethanol extracts of the leaf and root exhibited strong inhibitory effects against selected microbial strains. Each plant solvent extract suppressed microbial development in a distinct manner, and methanol and hydroethanol extracts inhibited microbial development more efficiently than aqueous extracts. Interestingly, water extracts had the least effective inhibitory effects across all strains. Notably, water extracts showed the weakest inhibitory effects against all strains. CONCLUSION: The current study demonstrated the efficacy of crude extracts of Cenchrus biflorus Roxb. against the tested strains of bacteria and fungi and also discussed their potential application as antibacterial agents for combating infectious diseases.
Current Status of Microalgae-based Food Products: Future Trends of Functional Ingredients.
Nörnberg ML, Schetinger LC, do Nascimento TC … +4 more , Pinheiro PN, Caetano PA, Jacob-Lopes E, Zepka LQ
Recent Pat Biotechnol · 2026 · PMID 40814888 · Publisher ↗
The use of microalgae in food and beverages is becoming increasingly popular as a viable way to develop products with enhanced nutritional profiles, offering positive health effects. In parallel, the plant-based food mar...
The use of microalgae in food and beverages is becoming increasingly popular as a viable way to develop products with enhanced nutritional profiles, offering positive health effects. In parallel, the plant-based food market is expanding due to the growing vegan, vegetarian, and flexitarian populations, prompting manufacturers to create innovative foods and techniques, such as the addition of microalgae to products. These functional and/or nutraceutical foods present an attractive option for consumers seeking plantbased alternatives. Although some challenges remain, this is a growing market. Furthermore, biotechnological processes are being utilized to optimize the production of microalgae with even more robust nutritional characteristics, thereby increasing their added value. This review was based on a structured literature search across major databases, applying predefined keywords and selection criteria to identify recent advances, regulatory aspects, and biotechnological developments in the field. These innovations hold significant potential to meet the rising demand for bioactive products and to propel a new era in the commercialization of microalgae-based products, a segment still underexplored in the current market. Additionally, progress in this sector depends on the development and protection of biotechnological innovations through patents, ensuring greater security and competitiveness in the industry.
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