Zhonghua Er Bi Yan Hou Ke Za Zhi[JOURNAL]
Sun
200 papers
[Culture of neural stem cells from rat olfactory bulb].
Chen FQ, Qiu JH, Wang JL … +2 more , Liu SL, Mi WJ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835822
OBJECTIVE: To establish the method of in vitro culture of neural stem cells (NSC) from rat olfactory bulb and investigate the characteristics of its proliferation and differentiation. METHODS: NSC from postnatal one-day...
OBJECTIVE: To establish the method of in vitro culture of neural stem cells (NSC) from rat olfactory bulb and investigate the characteristics of its proliferation and differentiation. METHODS: NSC from postnatal one-day (P1) and adult rat olfactory bulb were isolated and cultured in serum free media with epidermal growth factor (EGF) and fibroblast growth factor-basic (bFGF). Antibodies against NSC (nestin), neuronal (neuronal specific enolase, NSE), astrocytic (glial fibrillary acidic protein, GFAP) and oligodendrocytic (galactocerebroside, GalC) markers were used to identify NSC and specific neural cells differentiated from NSC with immunocytochemical staining. Growth curve of olfactory bulb NSC was measured using MTT method. RESULTS: Nestin immuno-positive NSC were isolated and cultured from P1 and adult rat olfactory bulb which could differentiate into neurons, astrocytes and oligodendrocytes. The forming rates of neurosphere from P1 and adult rat olfactory bulb were 20% approximately 30% and 0.1% respectively. The proliferation of olfactory bulb NSC depended on EGF and bFGF, in which EGF increased proliferation of cells stronger than bFGF. CONCLUSIONS: NSC with self-renewal capacity and potential multi-differentiation were cultured from P1 and adult rat olfactory bulb.
[Research of fibronectin, p53 and Ezrin protein with occurrence, progress and prognosis in the primary laryngeal carcinoma by the tissuechip technology].
Dong P, Li XY, Lu GZ … +4 more , Zhu ZH, Yu ZW, Jin B, Zhu JC
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835821
OBJECTIVE: To evaluate the predictive role of fibronectin, p81 (Ezrin protein) and p53 gene in primary laryngeal carcinoma, it's relationship with epidemiology(smoking), histological grading, surgical treatment, TNM stag...
OBJECTIVE: To evaluate the predictive role of fibronectin, p81 (Ezrin protein) and p53 gene in primary laryngeal carcinoma, it's relationship with epidemiology(smoking), histological grading, surgical treatment, TNM stage and prognosis were studied by the tissuechip technology. METHODS: The expression of fibronectin, p53 gene and p81 (Ezrin protein) on a series of 85 primary laryngeal carcinoma patients treated in our hospital between 1992 and 2000 was studied with tissuechip technology. The correlation of each score according to the intensity and percentage of labeled cells or intercellular substance with relevant clinical dada was statistically analyzed. RESULTS: Some cases were lost or boosted no tumor tissue in our tissuechip. Among the 70 cases available, 45.71% (32/70) of the specimens' basal membrane and extracellular matrix were strongly stained with fibronectin; there is statistical significance (P < 0.05) between primary tumor grading groups. Ezrin protein expressing rate is 87.3%, and the average percentage of its labeled cells is 53.68% (ranging from 0% to 100%, median is 58. 69%). There were significant difference between tumor grading groups, clinical early and late stages and 3-year survival rates (P < 0.05) after chi-square test. But no relation with smoking, gender, age and histological classes (P > 0.05). The average percentage of p53 positive cells is 21.6% (ranging from 0% to 90.3%, median is 5.85%) and 46.8% showed positive stains in our research. There was no statistical prominence in p53 protein demonstration between TNM stages, lymph node metastasis, 3-year survival rate, smoking, gender, age and histological classes (P > 0.05). CONCLUSIONS: The tissue microarray technique spent shorter time and less expense, and showed higher consistency in our essays. And the present study suggests fibronectin and p81 (Ezrin protein) could be the clinical discriminators in laryngeal carcinoma.
[Effects of basic fibroblast growth factor on protein kinase B activity and c-fos expression in CNE- I nasopharyngeal carcinoma cell line].
Wang XP, Sun LG, Liu N … +3 more , Yu HY, Zhang Y, Shan YQ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835820
OBJECTIVE: The relationship between basic fibroblast growth factor (bFGF) and phosphatidylinositol 3-kinase/protein kinase B(PI 3-K/PKB) signal pathway was studied during the course of bFGF regulating carcinoma epithelio...
OBJECTIVE: The relationship between basic fibroblast growth factor (bFGF) and phosphatidylinositol 3-kinase/protein kinase B(PI 3-K/PKB) signal pathway was studied during the course of bFGF regulating carcinoma epithelioid cell lines- I (CNE- I ) nasopharyngeal carcinoma cell proliferation through altering time of bFGF treatment. METHODS: PKB activities were measured by advanced Takai method. C-fos expression was examined by Western Blot. RESULTS: bFGF (50 ng/ml) significantly stimulated PKB activity,After pretreatment of Wortmannin for 1 h, the PKB activity decreases to the basal levels(P < 0. 05),compared with control group. After treatment of bFGF (50 ng/ml) at various time, the expression of c-fos is increased. The expression of c-fos increases to the peak when the time of bFGF treatment is 30 min. After pretreatment of Wortmannin for 1 h, the expression of c-fos decreases 20% when the time of bFGF treatment was 30 min and when the time of bFGF treatment was 45 min, the expression of c-fos decreases 35%. CONCLUSIONS: 1. PI 3-K and PKB mediate bFGF-induced signal transduction in CNE- I nasopharyngeal carcinoma cell line; PKB lies in downstream of PI 3-K. 2. bFGF induces the increased expression of c-fos through PI 3-K/PKB in CNE- I nasopharyngeal carcinoma cell line.
[Nasal alar reconstruction with a vascularized free auricular composite flap].
Qian YL, Zhang YX, Zhang YG … +1 more , Wang DR
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835819
OBJECTIVE: The plastic surgeons prefer to reconstruct nasal alar with free auricular composite flap because it well matches nasal tissue in contour, texture and color. However, the size of the free composite flap should...
OBJECTIVE: The plastic surgeons prefer to reconstruct nasal alar with free auricular composite flap because it well matches nasal tissue in contour, texture and color. However, the size of the free composite flap should be less than 1. 0 cm x 1.5 cm due to the limitation of revascularization. Our aim is to search for a surgical method which could be used to repair full-layer larger nasal alar defects. METHODS: A surgical technique was presented to repair nasal alar defect with a free auricular composite flap, which was vascularized by branches of superficial temporal artery. Briefly, the contralateral auricular composite tissue pedicled by superficial temporal vessels (3 to 4 cm in length) was harvested from region of helix crus and preauricular skin, which matched the arc of the nasal rim, and then transplanted onto the recipient area. The superficial temporal vessel pedicles were anastomosed to facial vessels via vessel grafts harvested from lateral circumflex femoral vessels, which were about 10 to 12 cm in length. RESULTS: Twelve cases were treated, among them, 11 cases were successfully repaired with satisfactory results, one case failed, possibly due to the bad condition of the patient's blood vessels. In our cases, the size of alar defect varied from 1.5 cm x 2.5 cm to 2.0 cm x 3.8 cm. The minimal auricular donor site deformities and inconspicuous scar were acceptable and could easily be hidden by hair. CONCLUSIONS: The technique of free auricular composite flap, which is vascularized by superficial temporal vessels, is ideal for nasal alar reconstruction.
[Effect of intranasal glucosteroid on the gene expression of interleukin-5 in nasal polyps].
Zhang L, Han DM, Zhou B … +2 more , Fan EZ, Liu ZY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835818
OBJECTIVE: To investigate the effect of intranasal glucosteroid treatment on the expression of interleukin (IL)-5 gene in nasal polyps. METHODS: Nasal polyps from topical steroid treated patients (n = 20) and untreated p...
OBJECTIVE: To investigate the effect of intranasal glucosteroid treatment on the expression of interleukin (IL)-5 gene in nasal polyps. METHODS: Nasal polyps from topical steroid treated patients (n = 20) and untreated patients (n = 20) were investigated with the technique of mRNA in situ hybridization. RESULTS: The majority of IL-5 mRNA positive cells in nasal polyps were lymphocytes or eosinophils. No statistical significance was found in the densities of IL-5 mRNA positive cells between allergic patients [(12.6 +/- 4.6)/0.25mm2] and nonallergic patients [(14.3 +/- 4.1)/0.25mm2] (t = -0.775, P > 0.05). Compared with the control group [(13.9 +/- 4.2)/0.25mm2], the density of IL-5 mRNA positive cells was decreased in the steroid-treated group [(10. 2 +/- 3.1)/0.25mm2], and the difference reached statistical significance (t = 3.114, P < 0.01). CONCLUSIONS: These findings indicate that topical steroid treatment may suppress the IL-5 gene expression, and steroids may inhibit eosinophil functions.
[Roles of epidermal growth factor and its receptor on the chronic tympanic membrane perforations].
Du XY, Lin RY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835817
OBJECTIVE: To evaluate the possible roles of epidermal growth factor(EGF) and its receptor (EGFR) on the chronic tympanic membrane perforations. METHODS: A phosphate buffer saline of EGFR was administered to a Gelfoam pl...
OBJECTIVE: To evaluate the possible roles of epidermal growth factor(EGF) and its receptor (EGFR) on the chronic tympanic membrane perforations. METHODS: A phosphate buffer saline of EGFR was administered to a Gelfoam pledget placed over chronic tympanic membrane perforations in guinea pigs. The EGFR of 10 specimens from the acquired middle ear cholesteatoma of the adjacent skin around perforation was examined by immunohistochemical SP method and computer image analysis. Results Complete closure of the tympanic membrane perforations was observed in 82.6% of EGF-treated ears, but only 33.3% in the controls(P < 0. 01). No case was led to middle ear cholesteatoma in the experiment group (0/23). The positive expression in the adjacent skin around perforation was (39.3 -/+ 7.4)%; and the normal external ear skin was (25.4 +/- 3.7)%; There were distinctly significant differences between the adjacent skin around perforation and the normal external ear skin (P < 0. 01). CONCLUSIONS: EGF is effective on closing chronic tympanic membrane perforations in the guinea pigs. Present data suggests that EGF-treated may induce the occurrence of middle ear cholesteatoma.
[Apoptosis in the immune response of inner ear].
Xu LJ, Gong SS, Wang JB … +5 more , Huang X, Song P, Yin SH, Chen P, Li H
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835816
OBJECTIVE: To investigate whether apoptosis is one of the mechanism in the immune response of inner ear,and to detect the expression of Fas, FasL, Bcl-2 and Bax in the inner ears. METHODS: Sixteen healthy, female guinea...
OBJECTIVE: To investigate whether apoptosis is one of the mechanism in the immune response of inner ear,and to detect the expression of Fas, FasL, Bcl-2 and Bax in the inner ears. METHODS: Sixteen healthy, female guinea pigs were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin (KLH), the KLH-immunized animals were inoculated with the same antigen, and the control animals were injected PBS through cochlea basal turn. The animals were sacrificed at 7 day after inner ear vaccination. Transmission electron microscopy was used to detect inner ear apoptotic cells, and paraffin sections of cochlea from animals were stained using a terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay to identify inner ear cells undergoing apoptosis. Immunohistochemistry method was used to detect the expression of Fas, FasL, Bcl-2 and Bax in the inner ears. RESULTS: The observation of electron microscopy had shown the features characteristic of apoptotic cells in the KLH-immunized inner ears but not in the control inner ears. TUNEL-positive cells were found in the KLH-immunized inner ears but not in the control inner ears. The positive cells were the hair cells in Corti's organ, and the marginal cells in the stria vascularis and the neurons in the spiral ganglion. Moreover under morphological analysis by light microscope, these cells had the features characteristic of apoptosis. High expression of Fas and FasL could be detected in Corti's organ, the stria vascularis, the spiral ligament and the neurons of the spiral ganglion in the KLH-immunized inner ears. A low expression of Fas could be detected in the stria vascularis and the neurons of the spiral ganglion in the control inner ears, but no cells staining positive for FasL were found in the control inner ears. No cells staining positive for Bcl-2 were found in the KLH-immunized animals but moderate expression of Bcl-2 could be detected in Corti's organ, the lateral wall and the neurons of the spiral ganglion in the control inner ears. High expression of Bax could be detected in Corti's organ, the lateral wall and the neurons of the spiral ganglion in the KLH-immunized inner ears. A low expression of Bax could be detected in the neurons of the spiral ganglion and no cells staining positive for Bax were found in Corti's organ, the lateral wall in the control inner ears. CONCLUSIONS: These findings suggest apoptosis is involved in the pathogenesis of the immune response of inner ear and Fas- FasL pathway is one of important signal transportation of the course and Bcl-2 and Bax have a critical role in the regulation of apoptotic cell death induced by the immune response of inner ear.
[How to reduce the incidence of complication and mortality in pediatric tracheobronchial foreign body patients].
Zhang J, Zhang YM
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835815
OBJECTIVE: To analyze the experience for the diagnosis and treatment of pediatric tracheobronchial foreign body cases, so to the incidence of complications and mortality. METHODS: 7260 tracheobronchial foreign body cases...
OBJECTIVE: To analyze the experience for the diagnosis and treatment of pediatric tracheobronchial foreign body cases, so to the incidence of complications and mortality. METHODS: 7260 tracheobronchial foreign body cases, from 1987 through 2003, were enrolled in this study. Early diagnosis, preoperative evaluation and perioperative treatment were strongly emphasized in this group of patients. RESULTS: 7618 patients received rigid bronchoscopy and 7260 cases were confirmed with tracheobronchial foreign bodies. The confirmed diagnosis rate is 95.3%. Among the 7260 cases, 7007 (96. 4% ) patients were diagnosed and received bronchoscopic foreign body removal within 24 hours. Different food is the most common foreign body aspirated in infants and toddlers (under 4 year old), whereas older children are more likely to aspirate other foreign bodies. Peanuts and sunflower seed (6862) were noted as the most common ones. Pen caps (398) are much less. All patients were evaluated before surgery after 1996. Complications occurred in 7 patients (0.1%) . The mortality rate is zero. 7165 cases' foreign body were successfully removed the first time of brochoscopy. 95 patients received more than one times bronchoscopy. 67 cases' foreign bodies were smoothly removed with second bronchoscopy. 16 cases received flexible bronchoscopic foreign body removal when rigid bronchoscopic procedure failed. 12 cases received chest surgery for foreign body removal. 6933 cases received bronchoscopy with surface anesthesia or without anesthesia, while 327 patients with general anesthesia combined local surface anesthesia. CONCLUSIONS: Rapid diagnosis and treatment can reduce the complication and mortality rate. So that early diagnosis, preoperative evaluation and perioperative treatment should be strongly emphasized.
[Diagnosis and treatment of obstructive sleep apnea hypopnea syndrome in children].
Zhang YM, Zhao J, Liu WY … +1 more , An JQ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835814
OBJECTIVE: To discuss the methods of diagnosis and treatment of obstructive sleep apnea hypopnea syndrome (OSAHS) in children. METHODS: 285 patients diagnosed OSAHS by X-ray for lateral rhinopharyngeal view, fibrorhinoph...
OBJECTIVE: To discuss the methods of diagnosis and treatment of obstructive sleep apnea hypopnea syndrome (OSAHS) in children. METHODS: 285 patients diagnosed OSAHS by X-ray for lateral rhinopharyngeal view, fibrorhinopharyngescopy and overnight polysomnography ( PSG) were reviewed retrospectively the clinical symptoms and signs. The patients whose apnea hypopnea index(AHI) > or = 5/h, blood oxygen's saturation reducing over 0.03 are diagnosed as OSAHS. 255/285 cases were performed adenoidectomy and/or tonsillectomy and 9 cases were treated with continuous positive airway pressure (CPAP). RESULTS: The major symptoms of patients with OSAHS are snoring in sleep, breathing opening mouth, apnea, hearing loss. 281/285 cases were diagnosed as adenoid and (or) tonsil hypotrophy. Two hundred- fifty five cases received surgery, adenoidectomy and tonsillectomy in 205, only adenoidectomy in 47, simple tonsillectomy in 3. The clinical symptoms of 248 cases improved evidently after operations. Improved rate is 97.2%. In 1-3 months after surgery,PSG were checked in 105 patients again: AHI, the percentage of the sleep time for saturation monitored by pulse oximetry < 0.90, the longest time of apnea are improved than pre-operation. 9 cases using CPAP received good effects, 7 of 9 cases used CPAP were in pre- and /or post- surgery. Two cases were obesity hypopnea syndrome. CONCLUSIONS: Children with OSAHS has signification them-self; PSG is the major means for diagnosing. Surgery is main method for treating; CPAP can be as the method of treating for serious OSAHS in preoperative preparation and postoperative management.
[Analyze of the clinical characteristics in children with the severe abnormal auditory brainstem response and normal distortion product otoacoustic emission].
Luo RZ, Wen RJ, Mai JN … +4 more , Zhong JW, Huang ZY, Chen Q, Yang S
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835813
OBJECTIVE: To explore the clinic and audiological characteristics of auditory neuropathy in children. METHODS: Between 2002 and 2004, ninety-eight cases with the severe abnormal results of ABR and the normal results of D...
OBJECTIVE: To explore the clinic and audiological characteristics of auditory neuropathy in children. METHODS: Between 2002 and 2004, ninety-eight cases with the severe abnormal results of ABR and the normal results of DPOAE were enrolled in the study group. The mean patient age was 9 months, with a range of 1 month to 5 years. According to the evaluation of pediatric neurologist, the high-risk factors of psychomotor development retardation were statistically analyzed whether the cases were accompanied by psychomotor development retardation or not. The cases with abnormal results of ABR and DPOAE and without abnormal conductive function were selected as the cochlea lesion group. Some same age children without hearing loss were subjected as normal control group. RESULTS: The psychomotor development retardation was performed in 83.67% of cases. Hypercholesterolemia of newborn was the common high-risk factor of psychomotor development retardation (43.88% ). Severe abnormal results of ABR were occurred in 66. 07% of ears. Prolonged latency of wave I - V ABR and acoustic stapedius reflex were observed in few cases. The DPOAE amplitudes at intermediate and high frequencies were normal or slightly descending. Occurring rate of DPOAE at intermediate and high frequencies was the same as in normal control group. CONCLUSIONS: Hypercholesterolemia of newborn is the most common cause of auditory neuropathy. The auditory test results are various in children with auditory neuropathy. This character is different from it in adults.
[Diagnosis and following-up and analysis of pathogenesis for congenital hearing loss].
Gong LX, Liu YJ, Nie WY … +5 more , Xing LL, Li YH, Li H, Qi YS, Liang C
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835812
OBJECTIVE: To investigate the incidence and the relevant high-risk factors of congenital hearing loss for infants from well-baby nursery and newborn intensive care unit. To explore the scientific and feasible mode of ear...
OBJECTIVE: To investigate the incidence and the relevant high-risk factors of congenital hearing loss for infants from well-baby nursery and newborn intensive care unit. To explore the scientific and feasible mode of early diagnosis, following-up and early intervention. METHODS: Two-stage universal newborn hearing screening ( UNHS ) were performed by using transient otoacoustic emission ( TEOAE ). Auditory brainstem responses ( ABRs) and 40 Hz auditory event related potentials (40 Hz-AERPs) was used as diagnostic tests for those failed re-screening at three-month old. Two kinds of infants who had abnormal ABRs or high-risk factors of hearing loss received following-up and routine audiological evaluation from 6 months to 3 years of age. Each infant with congenital hearing loss received personalized intervention. RESULTS: The incidence of congenital hearing loss among infants who received UNHS was 5.73 per thousand. The incidence of congenital hearing loss was 20.02 per thousand in NICU and 3.67 in WBN. CONCLUSIONS: Whether passed the UNHS or not , infants from NICU should receive ABR and 40 HzAERP tests. The following-up population includes infants with abnormal ABRs or high-risk factors of hearing loss. Following-up and early intervention should be personalized.
[Strengthening development of pediatric otolaryngology].
Bu XK
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Nov · PMID 15835811
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[Diagnosis and surgical management of Hashimoto's disease coexistent with thyroid carcinoma].
Ge JH, Yao Y, Zhao RL … +1 more , Hu JL
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Dec · PMID 15813020
OBJECTIVE: To study clinical assessment and management of the Hashimoto's disease coexistent with thyroid carcinoma. METHODS: A retrospective analysis in 182 cases of Hashimoto's disease treated from 1982 to 1998 were un...
OBJECTIVE: To study clinical assessment and management of the Hashimoto's disease coexistent with thyroid carcinoma. METHODS: A retrospective analysis in 182 cases of Hashimoto's disease treated from 1982 to 1998 were undertaken. The preoperation assessment, surgical management and the complication after operation had been studied. RESULTS: Eighteen cases of thyroid carcinoma were found in total of 182 patients. The coexistent rate of the Hashimoto's disease with thyroid carcinoma was 9.9% (18/182). Preoperative diagnostic accuracy was only 33.3% (6/18). The 5-year and 10-year survival rates were 88.9% (16/18) and 81.3% (13/16), respectively. Seven received second operation, 2 of them found cancer in the specimens . The 5 years survival rates of the differentiated thyroid cancer (88.2%) was higher than the undifferentiated thyroid cancer (0), but there were no statistical difference due to the small cases (P = 0.167). Two patients had local recurrence and 3 had metastases. They had salvage surgery, which was curative. Three patients with undifferentiated thyroid cancer died of remote metastases. Six patients suffered hypothyroidism. CONCLUSIONS: The preoperation diagnosis accuracy of Hashimoto's disease coexistent with thyroid carcinoma was low. A detail case history,computed tomography (CT), fine needle aspiration (FNA) can help the disease to be diagnosed. The surgical treatment is effective on patients with Hashimoto's disease coexistent with thyroid carcinoma.
[Salvage surgery for patients with metastatic squamous cell carcinoma involving carotid artery in head and neck].
Bian X, Xu ZG, Qi YF … +1 more , Tang PZ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Dec · PMID 15813019
OBJECTIVE: To study the surgical approaches for metastatic squamous carcinoma involving carotid artery in head and neck. METHODS: Fifty-seven patients with metastatic squamous carcinoma involving carotid artery were anal...
OBJECTIVE: To study the surgical approaches for metastatic squamous carcinoma involving carotid artery in head and neck. METHODS: Fifty-seven patients with metastatic squamous carcinoma involving carotid artery were analyzed retrospectively. Forty-eight were undergone peel-off tumor from carotid artery and another 9 with carotid artery resection due to being invaded in the middle layer of the carotid artery wall. None of them were received artery reconstruction. Postoperative radiotherapy was given to those who had radiation with the total dose less than 70 Gy or no radiotherapy, or gross tumor residue during operation. RESULTS: Of all the 57 patients, 53 had been death and only four survived without clinical lesion. One patient died of postoperative multi-organ failure, 10 of local recurrence, 19 of regional recurrence, 14 of distance metastases, 7 of carotid artery bleeding eroded by tumor, and the remaining 2 of unknown causes. Median survival was 13 months and 1, 2, 3, 5-year overall survival rate was 44.6%, 16.2%, 12.5%, 8.3% respectively, as estimated by the Kaplan-Meier approach. The recurrent rate of gross negative and positive were 26.7% and 50.0% respectively. 2-year survival rate of those were 22.2% and 0 respectively. Recurrent rate and 2-year overall survival rate in patients with and without postoperative radiotherapy was 46.4% and 21.4%, 73.1% and 15.4% respectively. CONCLUSIONS: It is necessary to perform aggressive salvage operation for patients with metastatic squamous cell carcinoma involving carotid artery. En blok removal of the tumor and the reasonable safe margin should be achieved. Unless the middle layer of the artery wall is involved, tumor peel-off is preferred to carotid resection to reduce the mortality and cerebrovascular complication. Postoperative radiotherapy reduces the recurrent rate and improves overall survival rate potentially.
[Clinical pathology feature and prognostic factors of cervical lymph node metastases in hypopharyngeal carcinoma].
Li XM, Di B, Shao YL … +4 more , Cheng JM, Ma HM, Gao CM, Shang YD
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Dec · PMID 15813018
OBJECTIVE: To investigate the risk clinicopathological factors of primary tumor in the prediction of cervical lymph node metastases and the cervical lymph node prognostic factors in hypopharyngeal squamous cell carcinoma...
OBJECTIVE: To investigate the risk clinicopathological factors of primary tumor in the prediction of cervical lymph node metastases and the cervical lymph node prognostic factors in hypopharyngeal squamous cell carcinoma. METHODS: A retrospective study was carried out to review the histopathological data from 98 hypopharyngeal squamous cell carcinoma patients. The relationship between histopathological parameters and cervical lymph node metastases were evaluated by means of a univariate chi2 test and multivariate stepwise logistic regression model. And the Cox regression model was used to define possible pathological parameters of neck node affecting survival including N staging, presence of cervical lymph node metastases and extracapsular nodal spread, size and number of positive neck nodes, and levels of positive neck nodes. RESULTS: The overall 5-year survival rate of patients with hypopharyngeal carcinoma was 28.6%. In a univariate and multivariate analysis, it was confirmed that size and growth pattern of primary tumor correlated to cervical lymph node metastases. In a multivariate Cox regression analysis, the most significant prognostic factors of cervical lymph node were the size of positive neck nodes and level involved. CONCLUSIONS: Cervical lymph node metastases were one of the most significant prognostic factors of hypopharyngeal carcinoma. The identification of patients at risk for cervical lymph node metastases and the management of the neck by coping with pathological factors of cervical lymph node affecting survival are very important to improve the treatment and prognosis of hypopharyngeal carcinoma.
[Clinical characteristic and treatment of laryngeal scleroma].
Sun Y, Liu YC, Chen ZJ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Dec · PMID 15813017
OBJECTIVE: To study the clinical manifestation and treatment of the laryngeal scleroma METHODS: Forty-three patients with laryngeal scleroma, from May, 1981 to December, 2002, with pathological diagnosed were reviewed re...
OBJECTIVE: To study the clinical manifestation and treatment of the laryngeal scleroma METHODS: Forty-three patients with laryngeal scleroma, from May, 1981 to December, 2002, with pathological diagnosed were reviewed retrospectively. The clinical features and the treatment methods of these patients were analysed. RESULTS: All 43 cases had hoarseness and 19 of them manifested dyspnea. In the larynx, the main scleromatous lesions were classified as atrophic stage in 2 cases, as granulomatous in 35 cases and as scarring in 6 cases. The preponderance lesions were located in the glottis in 13 cases, in the subglottis in 1 case, in both supraglottis and glottis in 18 cases, in both glottis and subglottis in 8 cases, and in all the three regions of the larynx in 3 cases. Twenty-four patients were treated with antibiotics, seven patients with surgery and two patients with radiotherapy. Eighteen of 24 patients who were treated with antibiotics were cured, and two of the recurrent patients were cured with a further period of antibiotics therapy. Four of the 24 patients with second or third degree of laryngeal obstruction required prophylactic tracheostomy. One patient in granulomatous stage with the second degree of laryngeal obstruction was cured with the combination of surgery and antibiotics therapy. Six patients in the scarring stage with laryngeal stenosis were cured with reconstruction surgery. One patient treated with radiotherapy recurred in the fourth year after treatment, and one patient failed to antibiotics therapy cured with radiotherapy combined with antibiotics therapy. CONCLUSIONS: Scleroma may involve the larynx and cause dysphonia and laryngeal obstruction. Antibiotics therapy is effective in most cases with laryngeal scleroma, and the long-time follow-up after treatment were necessary. Laryngeal reconstructions were necessary for the patients with cicatrical laryngeal stenosis.
[Diagnosis and management of sulcus vocalis].
Wen W, Zhou SM, Zhang RX … +5 more , Shen XH, Wang SM, Huang YD, Sun GB, Geng LP
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Dec · PMID 15813016
OBJECTIVE: To clarify the pathophysiology of sulcus vocalis and to develop a more rational approach to treatment. METHODS: Twenty-nine cases of sulcus vocalis patients were divided into three classification: Type I is a...
OBJECTIVE: To clarify the pathophysiology of sulcus vocalis and to develop a more rational approach to treatment. METHODS: Twenty-nine cases of sulcus vocalis patients were divided into three classification: Type I is a physiologic variant and no dysphonia (11 cases). Types II (sulcus vergeture, 13 cases) and III (sulcus vocalis, 5 cases) are characterized by severe dysphonia and loss of vibratory activity. Eighteen cases of dysphonia were treated by surgery and phonation training. The operations included fat injection into vocal cords (9 cases of types II and 1 cases of types III, including 1 case of types III of second operation), fat implantation into sulcus vergeture after incision (4 cases of types II and 1 cases of types III) and undermining of the mucosa and sulcus vocalis resection (4 cases of types III, including 1 case of second operation). Phonatory function and video laryngostroboscopic data were evaluated before and after surgery and phonation training treatment in 18 patients. The mean follow-up time was 15.3 months. RESULTS: Ten cases of types II had excellent results after fat injection into vocal cords (n = 6) and fat implantation into sulcus vergeture after incision (n = 4). Three cases of types II improved after fat injection into vocal cords. Three cases of type III had excellent results after sulcus vocalis resection. One case of type III had excellent results by Second operation (sulcus vocalis resection) after fat injection into vocal cord. One case of type III improved by Second operation (fat injection into vocal cords) after fat implantation into sulcus vergeture after incision. No postoperative complications were noted. CONCLUSION: Accurate classification of sulcus vocalis is important and then adapt treatment to different types. Fat implantation into sulcus vergeture to type II and sulcus vocalis resection to type III were the best choice methods.
[Analysis of related factors of nasosinusitis after radiotherapy for nasopharyngeal carcinoma].
Zhang XH, Huang JN, Fu XJ … +1 more , Zhan ST
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Dec · PMID 15813015
OBJECTIVE: To study the incidence, clinical features and related factors of nasosinusitis after radiotherapy for nasopharyngeal carcinoma. METHODS: Five hundred and thirteen patients with nasopharyngeal carcinoma were in...
OBJECTIVE: To study the incidence, clinical features and related factors of nasosinusitis after radiotherapy for nasopharyngeal carcinoma. METHODS: Five hundred and thirteen patients with nasopharyngeal carcinoma were included in the study, to observe the clinical manifestation and image changes before and after radiotherapy. The incidence and influencing factors of nasosinusitis after radiotherapy were analyzed. RESULTS: Among 513 patients, before radiotherapy, nasosinusitis was found in 51 patients (9.9%). After radiotherapy, another 401 nasosinusitis was found (401/462). The difference of incidence rate of nasosinusitis before and after radiotherapy was obvious (chi2 = 533.21, P < 0.01). The incidence rate of nasosinusitis in the end of radiotherapy, 3 months, 6 months, 12 months and 18 months after radiotherapy was 10.7% (43/401), 13.7% (55/401), 58.1% (233/401), 12.0% (48/401), 5.5% (22/401) respectively. The incidence rate of nasosinusitis after fractional radiotherapy and continuous radiotherapy was 35.7% (143/401), 64.3% (258/401) respectively. CONCLUSION: The incidence rate of nasosinusitis after radiotherapy is very high. It is influenced by the dose of radiotherapy, but it has no relation with the extension of nasopharyngeal carcinoma.
[Effect of immunotherapy on house dust mite allergen-specific IgG1 and igG4 antibodies in nasal secretion for patients with perennial allergic rhinitis].
Xiao SF, Wang QH
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Dec · PMID 15813014
OBJECTIVE: To investigate the effect of immunotherapy on allergen-specific IgG1 and IgG4 in nasal secretion for patients with nasal allergy. METHODS: Nasal secretion was collected with suction from normal subjects, patie...
OBJECTIVE: To investigate the effect of immunotherapy on allergen-specific IgG1 and IgG4 in nasal secretion for patients with nasal allergy. METHODS: Nasal secretion was collected with suction from normal subjects, patients with allergic rhinitis untreated and treated with immunotherapy. Indirect, non-competitive streptavidin-biotin ELISA was applied to analyze allergen-specific IgG1 and IgG4 in nasal secretion. RESULTS: Patients with nasal allergy both treated and untreated showed a higher concentration of IgG1 and IgG4 (Z = -3.623, -3.061, P < 0.01, respectively), treated group showed higher concentration of IgG1 and IgG4 than untreated group (Z = -2.453, -3.408, P < 0.01, respectively). IgG4 antibody specific to purified allergen Der f I in the treated group was higher than normal subjects (Z = -3.518, P < 0.01), both IgG1 and IgG4 antibodies specific to purified allergen Der f II in treated group were higher than normal subjects (Z = -2.366, -2.936, P < 0.01, respectively) and than untreated group (Z = -2.366, -2.937, P < 0.01, respectively). In the IgG subclasses of IgG1 and IgG4, there was positive correlation among crude mite extract, Der f I and Der f II. There was a positive correlation between IgG1 and IgG4 for all 3 kinds of allergens. There was a positive correlation between IgG1 in the nasal secretion and IgE in the serum and between IgG4 in the nasal secretion and IgE in the serum for crude mite extract. CONCLUSIONS: Patients with nasal allergy showed higher responsiveness to natural allergen exposure and produced higher level of IgG1 and IgG4 antibodies to specific allergens. Immunotherapy increased the concentration of IgG1 and IgG4 antibody in the nasal secretion, which might function as a blocking antibody to specific allergen.
[Expression profile of immune associated genes in nasal polyps].
Wang X, Dong Z
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Dec · PMID 15813013
OBJECTIVE: To investigate the expression profile of immune associated genes in nasal polyps by gene chip technology and to probe into the role of correlative genes in the immune pathogenesis of nasal polyps. METHODS: Mic...
OBJECTIVE: To investigate the expression profile of immune associated genes in nasal polyps by gene chip technology and to probe into the role of correlative genes in the immune pathogenesis of nasal polyps. METHODS: Microarray analysis was used to find the expressing profile of 491 immune associated genes in nasal polyps. The total RNAs were respectively extracted from four samples of nasal polyps and inferior turbinates, and then were reversely transcribed to cDNAs with incorporation of fluorescent dUTP as the hybridization probes. The mixed probes were then hybridized with two pieces of immune associated gene chip. It was scanned by laser scanner and the acquired image was analyzed by software. RESULTS: Eighty-seven genes were differently expressed in immune associated gene profile of nasal polyps, among which 45 genes were upregulated and 42 genes were down regulated. Fifteen genes were shown differential expression in both chips with 5 upregulated genes and 10 downregulated genes. The differentially expressed genes mostly involved in cytokines and their receptors, chemokines and their receptors, adhesion molecules, leukocytes differential antigens, immune signal transduction molecules, and still included some genes about complements and their receptors, immune transcription regulatory molecules, innate immune molecules and neural immune molecules. CONCLUSIONS: The differently expressed genes in immune associated gene chips will provide clues and theoretical foundation for the pathogenesis of nasal polyps. Furthermore IL-17 may have an important role in the occurrence of nasal polyps, and the role of innate immunity and immune signal transduction molecules in the pathogenesis of nasal polyps need further researches.
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