Recent Patents On Biotechnology[JOURNAL]
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200 papers
Renewable and Sustainable Biorefinery: A Patent Review.
Alexandre SA, Granjeiro PA, da Silva JA … +1 more , Goncalves DB
Recent Pat Biotechnol · 2026 · PMID 40176690 · Publisher ↗
BACKGROUND: Biorefineries can refer to forms of fuel production through renewable biomass derivatives, using different structures of lignocellulosic material, such as lignin, hemicellulose, and cellulose. From lignin, we...
BACKGROUND: Biorefineries can refer to forms of fuel production through renewable biomass derivatives, using different structures of lignocellulosic material, such as lignin, hemicellulose, and cellulose. From lignin, we can produce natural binders and adhesives, among other products. With hemicellulose, we can produce emulsifiers, resins, or lubricants, for example. Using cellulose, we can produce fuels, such as ethanol and biodiesel, or even solvents. Fuels from biorefineries can replace, totally or partially, non-renewable fuels that pollute the environment, such as oil. Considering the climate emergency, we are experiencing, the tendency to reduce the availability of oil, and the negative environmental impacts caused by it, fuels obtained through the processing of renewable plant materials present themselves as a good alternative to replacing fossil fuels. Firstgeneration ethanol (1G) can be obtained by fermenting, for example, sugar cane juice. Secondgeneration (2G) ethanol can be obtained by processing lignocellulosic waste. In this process, there must be pre-treatment and hydrolysis of the biomass before the fermentation and distillation processes. Third-generation ethanol (3G) can be obtained through the fermentation of substrate present in microalgae. Fourth-generation ethanol (4G), in turn, involves the integration of production processes from other generations, increasing the efficiency of 2G and 3G processes for ethanol production. OBJECTIVE: The objective of this study was to investigate the scenario of patent registrations filed both on the google patents platform and espacenet, which proposes the production of fuels from biorefineries, that are renewable and sustainable. METHODS: Although there are other lignocellulosic products originating from biorefineries, we will limit ourselves to patents aimed at the production of cellulosic ethanol. The search covered patents filed in the last 5 years (2019-2023). The 10 patents from each of the 3 biotechnological areas were selected, classified as agriculture, environment, and bioprocesses/bioengineering, totaling 30 patents to be analyzed. After selecting patents through the insertion of keywords and Boolean operators, the patents were selected by reading the title, its summary, and, finally, the full document to verify which were aligned with the study. RESULTS: Analysis of the documents revealed that, in most cases, China leads the way in patent applications involving the use of fuels, such as cellulosic ethanol, which are environmentally renewable and sustainable. The main strategies for the production of renewable and sustainable fuels in the context of biorefineries explore mechanisms for reusing agricultural waste, pre-treatment of residual biomass, and reuse of biorefinery waste, among other technologies. CONCLUSION: The future perspective is that the production of renewable and sustainable energy, such as that coming from biorefinery fuels, has solved its challenges and contributes to the growing global energy transition process. Analyzing and incorporating advances found through patent analysis into technological development provides mechanisms for better performance in the biorefinery sector. In addition to innovations, it is possible to analyze economic and environmental challenges, promoting integrated strategies that combine sustainability and commercial viability. Thus, the energy transition can be accelerated with solutions that increase the efficiency and technological innovation of biorefineries.
Development of a Novel Peptide with RGD Tumor Homing Motif: Evaluation of its Anticancer Potential in Hepatocellular Carcinoma and Colon Cancer Cells.
Mohamed RA, Marie OM, Badran DI … +2 more , Hammam OA, Ahmed HO
Recent Pat Biotechnol · 2025 · PMID 39916422 · Publisher ↗
BACKGROUND: Peptide-based therapy has emerged as a promising avenue for treating various disorders, and recent research has highlighted the potential of anti-cancer peptides (ACPs) in cancer treatment. In this context, t...
BACKGROUND: Peptide-based therapy has emerged as a promising avenue for treating various disorders, and recent research has highlighted the potential of anti-cancer peptides (ACPs) in cancer treatment. In this context, this study aimed to design a novel peptide incorporating a tumor-homing peptide (RGD) and C-amidation to enhance its anticancer activity, particularly against liver (HepG2) and colon (HCT-116) cancer cell lines. OBJECTIVES: The primary objective was to design a peptide with improved anticancer properties by leveraging the tumor-homing capabilities of RGD and enhancing its activity through C-amidation. The study sought to evaluate the cytotoxicity of the designed peptide against red blood cells (RBCs) and normal Vero cells. Furthermore, the anticancer efficacy of the peptide was assessed in hepatocellular carcinoma (HepG2) and colon cancer (HCT-116) cell lines. The specific objectives included examining the apoptotic induction and morphological changes in treated cells compared to untreated cells. METHODS: The peptide was designed using the ACPred-FL bioinformatics tool, and its cytotoxicity was assessed through hemolysis assays against RBCs and normal Vero cells. Anticancer activity was evaluated against HepG2 and HCT-116 cell lines. The analysis of apoptotic induction involved measuring the relative gene expression of oncogenic marker BCL2 and apoptotic markers (BAX, BID, CAS-8). Additionally, Cytopathological examination and Western Blot analysis were employed to study morphological changes and confirm the quantification of relevant markers. RESULTS: The designed peptide, consisting of twelve amino acids with a molecular mass of 1230.6233 Da and an isoelectric point of 9.81, exhibited low erythrocyte lysis and minimal toxicity to normal cells. The IC values demonstrated significant anticancer activity against both HepG2 (36.49±2.6 μg/mL) and HCT-116 (11.03±2.5 μg/mL) cell lines. Treated cells exhibited a significant decrease in the oncogenic marker BCL2 and an upregulation of apoptotic markers (BAX, BID, CAS-8). Western Blot analysis confirmed these results in addition to cytopathological examination that scattered apoptotic and degenerative changes. CONCLUSION: The designed peptide is considered a patent product that displayed remarkable anticancer activity against hepatocellular carcinoma and colon cancer cell lines, effectively modulating apoptotic and oncogenic markers. These findings highlight the potential of the peptide as a therapeutic agent for cancer treatment, emphasizing its clinical significance in combating liver and colon cancers. Nonetheless, further research and development are warranted to explore the translational potential of this peptide in clinical studies.
Construction of Immune Single Domain Antibodies Library for Development of Specific Nanobodies Using Phage Display Strategy.
El-Kalamawy HA, Awwad MH, Diab TM … +5 more , Okasha H, Abdel-Kareim AM, Marawan MA, Shoulah SA, El-Dabaa E
Recent Pat Biotechnol · 2025 · PMID 39840412 · Publisher ↗
BACKGROUND: poses a considerable global public health challenge. In Egypt, approximately 60% of the inhabitants in the Northern and Eastern areas of the Nile Delta are affected by this parasite, whereas the Southern reg...
BACKGROUND: poses a considerable global public health challenge. In Egypt, approximately 60% of the inhabitants in the Northern and Eastern areas of the Nile Delta are affected by this parasite, whereas the Southern region experiences a significantly lower infection rate of 6%. AIM: Construction of an immune phage display Nbs library based on the VHH framework for selecting -specific Nbs for seeking cost-effective, sensitive, and specific diagnostic tools for rapidly detecting mansoni. METHODS: Camel was immunized using soluble adult worm antigens (SAWP) for the production of Variable domains of heavy chains of camelid heavy-chain only antibodies (VHHs), which are known as nanobodies (Nb). The PBMCs repertoires VHH sequences library have been constructed with a high percentage of insertion and right orientation using pADL-23c phagmid and M13 phage followed by three rounds of bio-panning against SAWP using phage display technique. Evaluations using polyclonal phage ELISA and other techniques have been carried out to reveal the successful enrichment of anti-SAWP Nbs (VHH) clones. Evaluation of the diagnostic potentiality of these Nbs was carried out using ELISA on human serum samples confirmed for S. mansoni infection. Receiver Operator of Characteristics (ROC) curve analysis was used for discrimination between S. mansoni infection and both negative controls and the Fasciola hepatica group. RESULTS: Using monoclonal ELISA, Nbs of 22 clones out of 24 selected clones showed binding affinity to SAWP. The cutoff values of the produced anti- Nbs was > 0.19, leading to 80% sensitivity, 95% specificity, and 90% accuracy. Sequence analysis of three of these Nbs with high binding affinities showed diversity in their targets, considering their CDR3 aa sequences. CONCLUSION: This study successfully generated a diverse phage library enriched with anti-S. mansoni VHHs. The nanobodies produced exhibit high diagnostic potential for detecting S. mansoni infection in human patients, offering a promising avenue for the development of efficient diagnostic tools. The innovative approach described herein may have potential applications for patent considerations in the field of the field of diagnostic technology.
Carvacrol: Innovative Synthesis Pathways and Overview of its Patented Applications.
El Boukhari R, Fatimi A
Recent Pat Biotechnol · 2025 · PMID 39840411 · Publisher ↗
AIM: This research concerns the patentability of carvacrol; it could be helpful for researchers to easily identify any innovation in the biotechnological application of this monoterpene as well as other similar compounds...
AIM: This research concerns the patentability of carvacrol; it could be helpful for researchers to easily identify any innovation in the biotechnological application of this monoterpene as well as other similar compounds. BACKGROUND: Like thyme or oregano, several plants in the Lamiaceae family produce carvacrol. It is one of the secondary metabolites with several biological activities, including the improvement in plants' resistance and their protection. Carvacrol has many chemical properties, such as antioxidant and anti-microbial, which have made it interesting for multiple biotechnological applications in the fields of food, feed, pharmacology, and cosmetology. OBJECTIVE: We have made an attempt to demonstrate the value of carvacrol, first by studying quantitative data from patent documents, and then, through some relevant patents, we have tried to highlight the various fields of innovation related to the properties of carvacrol. METHODS: For the study, we have collected and sorted patent documents (i.e., patent applications and granted patents) from specialized patent databases, using "carvacrol" and some of its synonyms as keywords. The selected documents have included these keywords in their titles, abstracts, or claims. Then, thanks to patent analysis, we have tried to provide an overview of the useful properties of organic compounds. RESULTS: We have shown that about 90% of the patent documents studied have been published in the 2000s. The number of publications, which is constantly increasing, demonstrates the growing interest in carvacrol. Although the applications of carvacrol are varied, the data on the IPC classification show that most published innovations are concerned with formulations in the fields of health, food, and feed. The study of the most relevant patents has allowed us to highlight some developments in the extraction and synthesis of carvacrol and some examples of patents that illustrate the wide possibilities offered by the exploitation of carvacrol. Thus, we have discussed its use in the cosmetic, pharmaceutical, food, and agricultural fields. CONCLUSION: Carvacrol is a natural compound with beneficial properties. Several applications using this monoterpene have already been patented in different fields. However, the evolution of patentability has grown this past year and revealed the potential of carvacrol in biotechnology.
Role of Artificial Intelligence in Drug Discovery to Revolutionize the Pharmaceutical Industry: Resources, Methods and Applications.
Singh PK, Sachan K, Khandelwal V … +2 more , Singh S, Singh S
Recent Pat Biotechnol · 2025 · PMID 39840410 · Publisher ↗
Traditional drug discovery methods such as wet-lab testing, validations, and synthetic techniques are time-consuming and expensive. Artificial Intelligence (AI) approaches have progressed to the point where they can have...
Traditional drug discovery methods such as wet-lab testing, validations, and synthetic techniques are time-consuming and expensive. Artificial Intelligence (AI) approaches have progressed to the point where they can have a significant impact on the drug discovery process. Using massive volumes of open data, artificial intelligence methods are revolutionizing the pharmaceutical industry. In the last few decades, many AI-based models have been developed and implemented in many areas of the drug development process. These models have been used as a supplement to conventional research to uncover superior pharmaceuticals expeditiously. AI's involvement in the pharmaceutical industry was used mostly for reverse engineering of existing patents and the invention of new synthesis pathways. Drug research and development to repurposing and productivity benefits in the pharmaceutical business through clinical trials. AI is studied in this article for its numerous potential uses. We have discussed how AI can be put to use in the pharmaceutical sector, specifically for predicting a drug's toxicity, bioactivity, and physicochemical characteristics, among other things. In this review article, we have discussed its application to a variety of problems, including drug discovery, target structure prediction, interaction prediction, and binding affinity prediction. AI for predicting drug interactions and nanomedicines were also considered.
Nutritional Health Connection of Algae and its Pharmaceutical Value as Anticancer and Antioxidant Constituents of Drugs.
Mirza SS, Akbar S, Ijaz MU … +3 more , Morowvat MH, Ishaque A, Fatima K
Recent Pat Biotechnol · 2025 · PMID 39840409 · Publisher ↗
The marine environment is one of the major biomass producers of algae and seaweed; it is rich in functional ingredients or active metabolites with valuable nutritional health effects. Algal metabolites derived from the c...
The marine environment is one of the major biomass producers of algae and seaweed; it is rich in functional ingredients or active metabolites with valuable nutritional health effects. Algal metabolites derived from the cultivation of both microalgae and macroalgae may positively impact human health, offering physiological, pharmaceutical and nutritional benefits. Microalgae have been widely used as novel sources of bioactive substances. Bioactive polymers extracted from algae, such as algal fucans, Galatians, alginates phenolics, carotenoids, vitamin B12, and peptides possess antioxidant, anticoagulant, antimicrobial, antiviral, anti-inflammatory, anti-allergy, anticancer, and hypocholesterolemic properties. It emphasizes that using marine-derived compounds with bioactive properties as functional food ingredients may help promote human health and prevent chronic diseases. Utilizing bioactive compounds has demonstrated notable advantages in terms of effectiveness more than conventional treatments and therapies currently in use which is also proven from different patents about algal applications in different fields. Despite the availability of numerous microalgae-derived products catering to human health and nutrition in the market, there remains a lack of social acceptance and awareness regarding the health benefits of microalgae. Hence, this review aims to offer a comprehensive account of the current knowledge on anticancers, antioxidants, commercially available edible algal products and therapeutics isolated from algae.
Pharmacological Approaches and Innovative Strategies for Individualized Patient Care.
Verma AK, Singh K, Gupta JK … +2 more , Kumar S, Jain D
Recent Pat Biotechnol · 2026 · PMID 39838664 · Publisher ↗
Personalized medicine is an evolving paradigm that aims to tailor therapeutic interventions to individual patient characteristics. With a growing understanding of the genetic, epigenetic, and molecular mechanisms underly...
Personalized medicine is an evolving paradigm that aims to tailor therapeutic interventions to individual patient characteristics. With a growing understanding of the genetic, epigenetic, and molecular mechanisms underlying diseases, tailored therapies are becoming more feasible and effective. This review highlights the significant advancements in personalized medicine, focusing specifically on pharmacological strategies. The article explores the integration of genomics, transcriptomics, proteomics, and metabolomics in drug development and therapy optimization. Pharmacogenomics, the customization of drug therapy based on an individual's genetic makeup, receives particular emphasis. This leads to the identification of specific biomarkers that can predict therapeutic response, drug toxicity, and susceptibility to various diseases. Additionally, the development of patented drugs and personalized therapeutic approaches based on these discoveries underscores the innovation in this field. Together with computational tools and artificial intelligence, these advancements contribute to tailored treatment plans for patients with conditions such as cancer, cardiovascular diseases, and neurological disorders. We also highlight the challenges and ethical considerations in implementing personalized medicine, such as data privacy, cost-effectiveness, and accessibility. We outline future prospects and ongoing research in this field, emphasizing the importance of collaborative efforts between researchers, clinicians, pharmacists, and regulatory authorities.
Exploring the Therapeutic Potential of TROP2 Gene Silencing in Hepatocellular Carcinoma.
Yakoub F, Hassan H, Mamdouh S … +3 more , Aboushousha T, Rashidi FB, El-Desouky MA
Recent Pat Biotechnol · 2026 · PMID 39835546 · Publisher ↗
BACKGROUND: Trophoblast Cell Surface Antigen 2 (Trop2) is a transmembrane glycoprotein that has been implicated in the progression and metastasis of various cancers, including hepatocellular carcinoma (HCC). Targeting Tr...
BACKGROUND: Trophoblast Cell Surface Antigen 2 (Trop2) is a transmembrane glycoprotein that has been implicated in the progression and metastasis of various cancers, including hepatocellular carcinoma (HCC). Targeting Trop2 expression may represent a promising approach for the development of novel therapeutic strategies and patents. OBJECTIVES: This study aimed to investigate the effects of Trop2 knockdown using small interfering RNA (siRNA) on the phenotypic and molecular characteristics of the HepG2 liver cancer cell line. METHODS: HepG2 cells were transfected with different concentrations of Trop2-targeting siRNA (3 nM, 5 nM, and 7 nM) at various time intervals (6, 24, and 48 hrs). The expression of Trop2 was assessed by real-time PCR before and after transfection. The impact of Trop2 knockdown on cell apoptosis, migration, morphology, histopathological features, wound-healing assays, and microscopic analysis was examined. Additionally, the expression of the TPM1 gene was evaluated using immunohistochemical analysis. RESULTS: Trop2 mRNA level was significantly decreased in HepG2 cells in a time- and concentration-dependent manner following siRNA transfection. The downregulation of Trop2 resulted in a marked increase in apoptosis, a reduction in cell migration, and alterations in cell morphology and histopathological characteristics. Furthermore, the expression of the TPM1 gene was found to be upregulated in Trop2-knockdown HepG2 cells. CONCLUSION: These results highlight the potential of Trop2 as a therapeutic target for the management of hepatocellular carcinoma.
Exploration of Polyhydroxybutyrate (PHB) Production Potential of Photosynthetic Microbes: A Sustainable Source of Bioplastic.
Mirza SS, Yaseen K, Aslam S … +4 more , Fatima A, Morowvat MH, Ishaque A, Fatima K
Recent Pat Biotechnol · 2026 · PMID 39810444 · Publisher ↗
INTRODUCTION: The present study examined Polyhydroxy butyrate production (PHB) potential of different photosynthetic microbes such as and -PK under different nutrient conditions. Biodegradable bioplastics, such as Poly-...
INTRODUCTION: The present study examined Polyhydroxy butyrate production (PHB) potential of different photosynthetic microbes such as and -PK under different nutrient conditions. Biodegradable bioplastics, such as Poly-β-hydroxybutyrates (PHB), derived from these microbes provide a sustainable alternative to conventional petroleum-based nondegradable plastics. BACKGROUND: As the demand for clean and sustainable alternatives rises, bio-plastic is gaining attention as a viable substitute to conventional plastics. However, conventional sources of bio-plastic production have inherent limitations, which can be effectively addressed through the utilization of photosynthetic microbes e.g., microalgae, purple non sulphur bacteria. METHODOLOGY: The production of bioplastic was evaluated by cultivating the microalgae in BG-11, BBM and PNSB in synthetic growth media (MI, MII) with different nitrogen concentrations of 0%, 50% and 100%. The biopolymer (PHB) was obtained from all experiments in a wide range of concentration (7-42.8%) of dry cell weight (DCW). RESULTS: In this study, algal isolate SK1 demonstrated the highest PHB content (42.8%) in BBM under 100% nitrogen starvations rendering the bioplastic exceptionally compatible and suitable for eco-friendly applications. Additionally, various patents cited by different authors on different aspects of microbial bioplastic production. CONCLUSION: Nutrition depletion such as nitrogen scarcity induced stressful growth conditions that resulted in highest accumulation of the biopolymer PHB. Optimizing nitrogen availability is key to maximizing PHB production, making it a viable sustainable alternative to conventional plastics.
The Effect of Green-Synthesized Nanoparticles on Dental Caries-Causing Bacteria: A Systematic Review.
Sepahdar A, Selahbarzin B, Naini AV … +3 more , Jafari S, Moradi M, Shakib P
Recent Pat Biotechnol · 2026 · PMID 39698887 · Publisher ↗
OBJECTIVE: The primary objective of this review was to investigate the therapeutic implications of green-synthesized metallic nanoparticles, such as silver and zinc nanoparticles, in addressing dental caries, with a focu...
OBJECTIVE: The primary objective of this review was to investigate the therapeutic implications of green-synthesized metallic nanoparticles, such as silver and zinc nanoparticles, in addressing dental caries, with a focus on their antibacterial properties and potential to improve current treatment modalities. MATERIALS AND METHODS: In this study, a comprehensive search was conducted across multiple databases, including PubMed, Google Scholar, SID, Scopus, Medline, and Web of Science, as well as selected herbal medicine journals. The inclusion of herbal medicine journals was justified by the growing interest in green-synthesized nanoparticles derived from plant extracts, which are relevant to the focus of this review. Articles published between 2015 and 2023 were evaluated. To ensure a robust selection process, studies were assessed for quality using specific criteria beyond the AXIS checklist, including study design, sample size, and methodological rigor. Any discrepancies between reviewers were resolved through discussion or consultation with a third reviewer. This approach aimed to minimize bias and ensure the inclusion of high-quality studies that contribute meaningfully to the review's objectives. RESULTS: In this study, 22 clinical trials were reviewed, and the effectiveness of synthesized nanoparticles was demonstrated in nearly all of them. The nanoparticles found to be most effective against tooth decay-causing bacteria include silver, zinc, selenium, nickel, and copper nanoparticles. To provide greater clarity, key findings, such as nanoparticle size, minimum inhibitory concentration (MIC) values, and specific antibacterial effects, are presented in a table. Additionally, a more in-depth comparison was made between these nanoparticles, focusing on their relative effectiveness under different conditions. For example, silver nanoparticles were consistently effective across various trials due to their strong antimicrobial properties, while zinc and copper nanoparticles showed efficacy in specific concentrations and against particular bacterial strains. Furthermore, green-synthesized nanoparticles were compared with those produced using traditional methods, evaluating factors, such as effectiveness, scalability, and safety, to provide a comprehensive understanding of their potential in dental caries treatment. CONCLUSION: According to the literature review, nanoparticles with antibacterial properties have the potential to serve as an alternative or complement to conventional chemical treatments for combating tooth decay. Future research should focus on exploring specific types of nanoparticles, such as green-synthesized metallic nanoparticles (e.g., silver and zinc), which have shown promising antibacterial effects, Many of these ideas are patentable. Additionally, more emphasis should be placed on optimizing synthesis methods that enhance biocompatibility and scalability for clinical use. The development of affordable treatment options using medicinal plants for green synthesis is particularly promising and should be further explored to make nanoparticle-based therapies more accessible in clinical settings.
Biosurfactants Used in the Bioremediation of Soils Contaminated with Hydrocarbons - Overview of the State of the Art and Future Perspectives.
de Oliveira MA, Tarabal VS, Silva FF … +7 more , Livio DF, da Silva DR, Parreira AG, Goncalves DB, da Silva JA, Guimaraes PPG, Granjeiro PA
Recent Pat Biotechnol · 2026 · PMID 39698886 · Publisher ↗
BACKGROUND: The increasing industrialization and hydrocarbon use have led to concerning soil contamination. Oil spills and improper disposal of oily waste pose threats to ecosystems and human health. The recovery of thes...
BACKGROUND: The increasing industrialization and hydrocarbon use have led to concerning soil contamination. Oil spills and improper disposal of oily waste pose threats to ecosystems and human health. The recovery of these environments is essential, but separating oily components from soil remains challenging. Current bioremediation strategies using synthetic surfactants can cause secondary contamination. Microbial biosurfactants, which are biodegradable and low in toxicity, emerge as promising solutions, and this study reviews methods for utilizing these biosurfactants in the environmental bioremediation of hydrocarbons. OBJECTIVE: This study explores the efficient and eco-friendly use of biosurfactants for hydrocarbon- contaminated soil management, providing a market-oriented analysis of recent patents and trends, and highlighting the transition from academic research to industrial applications. METHODS: The methodology involves an extensive literature review, careful selection of recent studies and patents on biosurfactants in hydrocarbon bioremediation, critical analysis of in-situ and ex-situ application methods, assessment of commercial viability, and synthesis of findings to contribute to sustainable solutions in contaminated environments. CONCLUSION: The present study demonstrates the extensive applicability of biosurfactants across various industrial sectors. The increasing interest in incorporating biosurfactants into industrial processes is driven by the pressing need for sustainable solutions to address tangible market challenges. Notably, the cosmetics industry exhibited the highest number of patents related to the use of biosurfactants, underscoring its significant role in advancing the adoption of these environmentally friendly agents. This trend highlights the critical demand for sustainable alternatives in product formulations and underscores the pivotal role of biosurfactants in fostering eco-innovation within the industry.
Antifungal Activity of Alcoholic Extract of on Fungi-Contaminated Dairy Products.
Fallahi F, Naghdifar S, Irani ZS … +3 more , Kazemi SMM, Shakib P, Madani M
Recent Pat Biotechnol · 2026 · PMID 39698885 · Publisher ↗
BACKGROUND: One of the main sources of contaminated dairy products is moldy fungi, specifically species of and . This study aimed to evaluate the effect of the alcoholic extract of plant on the growth of molds contamin...
BACKGROUND: One of the main sources of contaminated dairy products is moldy fungi, specifically species of and . This study aimed to evaluate the effect of the alcoholic extract of plant on the growth of molds contaminating dairy products in Isfahan. MATERIALS AND METHODS: In this patent research, 200 samples of dairy products were gathered from different areas of Isfahan city, including 70 samples of cheese, 60 samples of buttermilk, 40 samples of yogurt, 20 samples of curd, and 10 samples of cream. The antifungal activity of plant was investigated by the diffusion method in disc, well, and agar dilution in various concentrations. Minimum inhibitory concentration (MIC) and minimum fatal concentration (MFC) were also determined. , and fungi were the most commonly found fungal contaminants of this investigation. Antifungal activity was not observed by disc diffusion and well diffusion methods. RESULTS: In the agar dilution method, ethanolic and methanolic extracts of stem and leaves in concentrations of 80, 60, 40, and 30 mg/ml, and ethanolic and methanolic extracts of plant bulbs in concentrations of 60 and 30 mg/ml revealed antifungal activity against , and . The MIC of stem and leaf ethanol extracts and onion ethanol for Aspergillus niger was 18.7, and for Penicillium notatum and Penicillium chrysogenum, it was 37.5, 37.5, 37.5, and 37.5 mg/ml. Fungal contamination of dairy products is a serious threat to the public health of society. Therefore, identifying medicinal plants with antifungal activity can be an effective step in preventing fungal contamination and increasing the shelf life of these products. CONCLUSION: The results of this research have shown that the Allium jesdianum plant can inhibit the growth of Aspergillus niger, Penicillium notatum, and Penicillium chrysogenum.
Antimicrobial Effect of Formononetin Against the Periodontal Pathogens and .
Delfan N, Moghadam MD, Shakib P … +2 more , Sepahdar A, Naghibeiranvand Z
Recent Pat Biotechnol · 2026 · PMID 39698884 · Publisher ↗
BACKGROUND: Among the bioactive isoflavones identified from different plants is formononetin. Formononetin's antioxidant, anti-inflammatory, and anti-cancer qualities have all received a lot of attention lately. The goal...
BACKGROUND: Among the bioactive isoflavones identified from different plants is formononetin. Formononetin's antioxidant, anti-inflammatory, and anti-cancer qualities have all received a lot of attention lately. The goal of the current investigation was to examine formononetin's antifungal and antibacterial activity against and , respectively. METHODS: The present study determined the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) of formononetin, chlorhexidine, formononetin + chlorhexidine, Formononetin + nystatin, and nystatin against and isolates. Next, SPSS version 25 was used to analyze the data. A significance cutoff of P<0.05 was taken into account. RESULTS: Chlorhexidine + formononetin and formononetin alone had the greatest and lowest minimum inhibitory concentrations (MIC) against , at 6.6 and 18.3 μg/ml, respectively. With MIC values of 0.66 and 8.3 μg/ml against a isolate sensitive to nystatin, the combination of formononetin + nystatin and formononetin alone was shown to have the greatest and lowest anti-candida effects. CONCLUSION: The findings demonstrated a considerably greater antibacterial effect (P<0.05) for the combinations of formononetin + nystatin and chlorhexidine as compared to nystatin and chlorhexidine alone.
Comparison of the Characteristics of Circulating Small Extracellular Vesicles Isolated by Ultracentrifugation and a Commercial Kit.
Afrisham R, Farrokhi V, Moradi R … +1 more , Alizadeh S
Recent Pat Biotechnol · 2025 · PMID 39473100 · Publisher ↗
INTRODUCTION: The market offers a wide range of extracellular vesicles (EVs) isolation products, but their lack of standardization is a concern. Therefore, it is important to carefully assess the quality of the EVs obtai...
INTRODUCTION: The market offers a wide range of extracellular vesicles (EVs) isolation products, but their lack of standardization is a concern. Therefore, it is important to carefully assess the quality of the EVs obtained using these products to patent the ideal method. In this study, we compared the EXOCIB kit with the ultracentrifuge method, which is considered the gold standard for small EV isolation. METHODS: After overnight fasting, small plasma EVs were extracted from four individuals using both the ultracentrifuge and the EXOCIB kit methods. The pooled EVs were then compared for the presence of the cluster of differentiation 63 (CD63) protein using the western blot analysis, and their size and zeta potential were performed by Dynamic Light Scattering (DLS). In addition, the size and morphology of small EVs were determined by using the Transmission Electron Microscopy (TEM) technique. RESULTS: An average hydrodynamic size of 135.7 nm and a zeta potential of -6.33 Mv at 25°C was found for small EVs isolated by the ultracentrifuge, whereas the kit method resulted in small EVs with a hydrodynamic size of 102.8 nm and a zeta potential of -0.907. Notably, the size of the particles in the kit samples was smaller compared to those obtained through the ultracentrifuge (P < 0.001). The western blot method confirmed the expression of CD63 in both methods, so the ultracentrifuge yielded small EVs with a higher level of purity compared to the kit-based approach (P = 0.036). CONCLUSION: The DLS findings revealed the existence of vesicles within the appropriate size range for small EVs like exosomes in both isolation techniques. The results of the western blot analysis, in conjunction with DLS, displayed that the ultracentrifuge method extracted small EVs with a greater degree of purity than the kit-based approach.
Curcumin-assisted Preparation of α-FeO@TiO Nanocomposites for Antibacterial and Photocatalytic Activity.
Kala A, Saini K, Kimothi S … +3 more , Verma R, Kar KK, Chamoli P
Recent Pat Biotechnol · 2025 · PMID 39428940 · Publisher ↗
BACKGROUND: Harmful microorganisms like pathogens significantly impact human health. Meanwhile, industrial growth causes pollution and water contamination by releasing untreated hazardous waste. Effective treatment of th...
BACKGROUND: Harmful microorganisms like pathogens significantly impact human health. Meanwhile, industrial growth causes pollution and water contamination by releasing untreated hazardous waste. Effective treatment of these microorganisms and contaminants is essential, and nanocomposites may be a promising solution. The present attempt demonstrates the green synthesis of α-FeO@TiO nanocomposites (FTNCs) for the effective treatment of pathogens and organic contaminants. METHODS: The FTNCs have been synthesized through a green approach utilizing curcumin extract. Curcumin (Turmeric) extract (TEx) was prepared by washing, drying, and crushing 5 g of turmeric, then boiling it in 100 mL distilled water at 70°C for 1 hour. Metal salts (Fe+/Ti, 2:1) were added to 100 mL of TEx under continuous stirring at 70°C for 24 h. The solution was rinsed and dried at 80°C overnight and heated at 300°C for 3 h to remove impurities. RESULTS: Synthesized FTNCs have been tested for the potent antibacterial activity against both Gram-positive ( and Gram-negative bacteria (). Observations discovered noteworthy inhibition of both Gram-positive and Gramnegative bacteria by FTNCs. Furthermore, the FTNCs system shows the energy band gap of ~2.6 eV which may suppress electron recombination, thereby enhancing photocatalysis. The photo-degradation is examined against Evans blue (EB) and Congo red (CR) dyes under UV and visible light (125 W) irradiation. The remarkable photocatalytic degradation efficiency (DE) for CR reached ~67.4% in 60 min. CONCLUSION: A simple green approach has been demonstrated for the synthesis of the FTNCs using curcumin-mediated reduction. As prepared FTNCs have been evaluated for potent antibacterial activity against both Gram-positive () and Gram-negative bacteria (). The results show that the highest zone of inhibition diameter values have been obtained for 5 mg/mL concertation of FTNCs of ~14, 22, 18, 21, and 20 and 29 mm for , and respectively. Additionally, FTNCs demonstrate remarkable photocatalytic degradation efficiency against EB and CR dyes under UV (125 W) irradiation, achieving 56, 67% degradation within 60 min, respectively. The findings indicate that FTNCs show long-term antimicrobial effectiveness and potential for water treatment through photocatalysis. This examination highlights recent advancements in intellectual property rights (IPR) and patent strategies, shedding light on how patents influence eco-friendly synthesis and the development of multifunctional, high-performance nanocomposites.
Current Scenario and Global Perspective of Sustainable Algal Biofuel Production.
Tripathi G, Hussain A, Irum … +6 more , Firdaus S, Dubey P, Ahmad S, Ashfaque M, Mishra V, Farooqui A
Recent Pat Biotechnol · 2025 · PMID 39390829 · Publisher ↗
Industrialization and globalization have increased the demand for petroleum products that has increased a load on natural energy resources. The escalating fossil fuel utilization has resulted in surpassing the Earth's ca...
Industrialization and globalization have increased the demand for petroleum products that has increased a load on natural energy resources. The escalating fossil fuel utilization has resulted in surpassing the Earth's capacity to absorb greenhouse gases, necessitating the exploration of sustainable bioenergy alternatives to mitigate emissions. Biofuels, derived from algae, offer promising solutions to alleviate fossil fuel dependency. Algae, often regarded as third-generation biofuels, present numerous advantages owing to their high biomass production rates. While algae have been utilized for their bioactive compounds, their capability as biomass for the production of biofuel has gained traction among researchers. Various biofuels such as bio-hydrogen, bio-methane, bio-ethanol, bio-oil, and bio-butanol can be derived from algae through diverse processes like fermentation, photolysis, pyrolysis, and transesterification. Despite the enormous commercial potential of algae-derived biofuels, challenges such as high cultivation costs persist. However, leveraging the utilization of algae byproducts could improve economic viability of biofuel production. Moreover, algae derived biofuels offer environmental sustainability, cost-effectiveness, and waste reduction benefits, promising novel opportunities for a more sustainable energy future. Moreover, advancements in the field could lead to patents that drive innovation and commercialization in algae-based biofuel technologies.
Relevance of Microorganisms in Causing Rain and Snow.
Kaul R, Devi S, Sharma M … +1 more , Chand S
Recent Pat Biotechnol · 2025 · PMID 39354771 · Publisher ↗
Various natural phenomena (such as solar fluctuations, oceanic patterns, volcanic eruptions, and tectonic movements) alongside human activities (including deforestation, CO and CO emissions, and desertification) contribu...
Various natural phenomena (such as solar fluctuations, oceanic patterns, volcanic eruptions, and tectonic movements) alongside human activities (including deforestation, CO and CO emissions, and desertification) contribute to ongoing climate change and subsequent global warming. However, human actions significantly exacerbate global warming, amplifying its adverse impacts worldwide. With rising temperatures, water evaporation from water bodies and soils intensifies, leading to heightened water scarcity, particularly in drought-prone regions. This scarcity compounds rainfall deficits, posing significant challenges. Precipitation, essential for the biosphere's hydrological cycle, replenishes much of the world's freshwater. It occurs when condensed water vapor in the atmosphere falls back to Earth as rain, drizzle, sleet, graupel, hail, or snow due to gravity. Literature highlights the indispensable role of microbial populations in this process, termed bio-precipitation. This phenomenon begins with microbial colonization on plant surfaces, with colonies subsequently dispersed into the atmosphere by winds, triggering ice crystal formation. Through their ice nucleating property, these microbes facilitate the growth of larger ice crystals, which eventually melt and precipitate as rain or snow. This mechanism aids in nutrient transfer from clouds to soil or vegetation. Pseudomonas syringae stands out as the most notable microorganism exhibiting this ice-nucleation property, serving as the primary source of ice nucleators driving bio-precipitation. Despite limited literature on "rain and snow-causing microorganisms," this review comprehensively explores the conceptual background of bio-precipitation, the involved bioprocesses, and the critical role of bacteria like P. syringae, offering insights into future research directions and patent innovations.
A Patent Landscape on Methane Oxidizing Bacteria (MOB) or Methanotrophs.
Bokad A, Telang M
Recent Pat Biotechnol · 2025 · PMID 39350426 · Publisher ↗
Methane-oxidizing bacteria (MOB) or methanotrophs are a category of bacteria that rely on methane as their primary carbon and energy source. Methane is the second most abundant greenhouse gas after carbon dioxide and is...
Methane-oxidizing bacteria (MOB) or methanotrophs are a category of bacteria that rely on methane as their primary carbon and energy source. Methane is the second most abundant greenhouse gas after carbon dioxide and is comparatively far more potent in trapping heat in the atmosphere. MOBs are important microorganisms in the global carbon cycle where they play a crucial role in the oxidation of methane. The present review provides a comprehensive patent landscape on technology development using MOB. The first patent in this technology domain was recorded in 1971, with a notable surge in activity observed in 2020. A detailed patent analysis revealed that the early inventions were mainly focused on the production of various metabolites and bioremediation using MOB. In the later years, patents were filed in the area of identification of various species of MOB and their large-scale production. From 2010 onwards, consistent patent filing was observed in the genetic engineering of MOB to enhance their methane oxidizing capacity. The United States and China have emerged as the global leaders in terms of patent filing in this technology space. Precigen Inc. and Exxon Research Engineering Co., US were the top patent assignees followed by the University of Tsinghua and Calysta Inc. The Highest number of patent applications have claimed metabolite production by using MOB followed by their use in bioremediation. Methylosinus has emerged as the predominant microorganism of choice for methane oxidation applications.
The Landscape of Products for Diabetic Peripheral Neuropathy: A Scientific and Patent Systematic Review.
Dos Santos Barros G, Dos Passos Menezes P, de Cassia Silva S … +1 more , da Silva GF
Recent Pat Biotechnol · 2025 · PMID 39253935 · Publisher ↗
BACKGROUND: Diabetic peripheral neuropathy (DPN) is a complication of diabetes that occurs in 40 - 60 million individuals worldwide and is associated with other chronic diseases. However, there are no review studies that...
BACKGROUND: Diabetic peripheral neuropathy (DPN) is a complication of diabetes that occurs in 40 - 60 million individuals worldwide and is associated with other chronic diseases. However, there are no review studies that present the state-of- the- art and technologies developed to circumvent this important health problem. MATERIALS AND METHODS: This review was conducted based on scientific papers and patents. The papers were retrieved from Lilacs, PubMed, and Web of Science databases, and the patents from INPI, ESPACENET, WIPO, and GOOGLE PATENTS. Thus, a sample consisting of 14 scientific articles and 667 patents was analyzed. RESULTS: From the analysis of the data, we drew an overview of the development of biomedical technologies for DPN and detected the pioneering spirit of China, the USA, and Japan in the area, with a focus on the treatment of DPN. Based on this, we carried out a SWOT analysis to help direct future efforts in the area, which should focus primarily on developing technologies for prevention, early diagnosis, and, above all, cure of the disease to reduce the important impact of this disease in various sectors of society. CONCLUSION: This study finds a concentration of diabetic peripheral neuropathy products, especially therapeutic drugs, in high-income countries. It highlights the need for global collaboration and strategic focus on therapeutic adherence and preventive strategies to effectively manage DPN.
The Role of Mycorrhizal Fungi in Orchids.
Kaur S, Deb A
Recent Pat Biotechnol · 2025 · PMID 39253934 · Publisher ↗
BACKGROUND: In nature, orchid plants are obligate myco-heterotrophs, and rely on mycorrhizal nutrient resources to grow and sustain in the wild, until they become physiologically active photosynthetic plants. Their seeds...
BACKGROUND: In nature, orchid plants are obligate myco-heterotrophs, and rely on mycorrhizal nutrient resources to grow and sustain in the wild, until they become physiologically active photosynthetic plants. Their seeds lack nutrient reserves and receive the necessary carbon from symbiotic fungi during germination. A mycorrhizal fungus provides nutrients, especially sugars, as well as water to the corresponding host plant. The range and distribution of orchid mycorrhizal fungi influence the survivability of orchid populations in their natural habitats. Mycorrhizae form symbiotic connections with the parenchymatous tissues of the roots of orchid plants. That the symbiotic orchid mycorrhiza can invade through roots of orchid seedling, raised , has been patented. OBJECTIVE: The objective of this study was to examine the presence of mycorrhiza in the roots of during the vegetative phase. METHODS: Fresh roots were hand-sectioned, and thin sections were observed under the microscope to locate the presence of mycorrhiza. Simultaneously, to observe the expansion of mycorrhiza in the cortical region. RESULTS: During the vegetative phase of plant growth, a peloton-like structure forms within the cortical region of the orchid roots. Mycorrhizae was observed to be distributed throughout the cortical layer of the root. CONCLUSION: This communication reviews the role of mycorrhiza in orchid plants.
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