Journal Of Oleo Science[JOURNAL]
Sun
200 papers
Transition of trans Fatty Acid Contents of Margarines, Fat Spreads and Shortenings in Japan.
Iida Y
J Oleo Sci · 2025 · PMID 40175115 · Publisher ↗
Margarine, fat spread, and shortening made with partially hydrogenated fats and oils as their raw materials have been known as foods containing trans fatty acids. Due to concerns about the health effects of trans fatty a...
Margarine, fat spread, and shortening made with partially hydrogenated fats and oils as their raw materials have been known as foods containing trans fatty acids. Due to concerns about the health effects of trans fatty acids, the content of trans fatty acids in these products has now been reduced to around 1 g/100 g (median value) through the use of alternative oils and fats, which is about the same level as that for vegetable oil used as main raw material. In this review, we summarized the trends in trans fatty acid content in products manufactured in Japan from 1969 to 2022.
Molecular Nutritional Research for Effective Utilization of Marine Lipid-soluble Components.
Hosokawa M
J Oleo Sci · 2025 · PMID 40175114 · Publisher ↗
Marine organisms contain unique lipid-soluble components. Therefore, we focused on the health benefits of these lipid-soluble components and conducted molecular nutritional studies. Fucoxanthin (Fx) is a typical marine c...
Marine organisms contain unique lipid-soluble components. Therefore, we focused on the health benefits of these lipid-soluble components and conducted molecular nutritional studies. Fucoxanthin (Fx) is a typical marine carotenoid, found in brown seaweeds, such as Undaria pinnatifida (Wakame) and Saccharina japonica (Makonbu), and we demonstrated its anti-obesity and anti-diabetic effects in animal models. As the molecular mechanism for anti-diabetic effect, dietary Fx has found to activate insulin signaling pathways and glucose transporter 4 (GLUT 4) in the skeletal muscles of diabetic/obese KK-A mice. Notably, Fx promoted GLUT4 translocation in the soleus muscle, up-regulated GLUT4 expression in the EDL muscle, and prevented and improved hyperglycemia through effective glucose uptake depending on the muscle types. On the other hand, n-3 docosapentaenoic acid (n-3 DPA), an n-3 poly unsaturated fatty acid found in salmon and trout, is converted to EPA and DHA in cultured cells. The intracellular conversion of n-3 DPA differed different among cells derived from macrophages, liver, and intestines. n-3 DPA markedly down-regulates the mRNA expression of pro-inflammatory factors in activated macrophages. The suppressive effect of n-3 DPA on IL-6 mRNA expression was similar to that of DHA, but stronger than that of EPA. In addition, we demonstrated that n-3 PUFA-binding phosphatidylglycerol (PG) exhibited anti-inflammatory effects against activated macrophages, and that the effect was stronger than that of n-3 PUFA-phosphatidylcholine (PC). Furthermore, n-3 PUFA-PG significantly increased the intracellular EPA and DHA content compared to n-3 PUFA-PC treatment and induced Nrf2 activation. n-3 PUFA-PG, which enhances intracellular PUFAs, is contained in several microalgae such as Phaeodactylum tricornutum. It can also be enzymatically prepared and is expected to be used as a new functional lipid.
Effects of Fingered Citron (Citrus medica var. sarcodactylis) Essential Oil on Improvement in Diet-Induced Hyperlipidemia Syrian Hamsters.
Yang KM, Chen HC, Chuang CH … +2 more , Chiang YC, Lin LY
J Oleo Sci · 2025 · PMID 40024785 · Publisher ↗
Preventing hyperlipidemia and the risk of cardiovascular disease are attractive to public health. Essential oils are extremely promising nutrients for use in the treatment of hyperlipidemia, whose effectiveness is closel...
Preventing hyperlipidemia and the risk of cardiovascular disease are attractive to public health. Essential oils are extremely promising nutrients for use in the treatment of hyperlipidemia, whose effectiveness is closely related to its volatile composition. We extracted fingered citron essential oil (FCEO) with steam distillation, analyzed the chemical composition, and evaluated its effects on hyperlipidemia. We identified 25 volatile compounds of FCEO with GC/MS, of which the main constituents were limonene and γ-terpinene. This study explored the protective effects of FCEOs against diet-induced hyperlipidemia Syrian hamsters. FCEOs treatment ranges from 0.03% to 0.05% with a daily diet. As of 12 weeks later, we found that the administration of the FCEOs improved the serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) levels (p < 0.05). Further, LDL-C/HDL-C (high-density lipoprotein cholesterol) ratios were significantly reduced (39.02-68.07 vs. 80.27). Simultaneously, the FCEOs had improved lipid metabolism and histopathology in the liver. These actions suggest the potential of FCEO as a valuable source of nutraceuticals in diet-based therapies.
Evaluation of Nutritional, Phytochemical, Fatty Acids and Mineral Composition of Eremurus robustus Leaves in Bradost Mountain, Kurdistan Region of Iraq.
Abdulwahid-Kurdi SJ
J Oleo Sci · 2025 · PMID 40024784 · Publisher ↗
One of the wild herbs that grows naturally in the springtime in Kurdistan's mountainous regions is Eremurus robustus. The study aimed to detect the proximate analysis, polyphenols, fatty acid profile, and mineral composi...
One of the wild herbs that grows naturally in the springtime in Kurdistan's mountainous regions is Eremurus robustus. The study aimed to detect the proximate analysis, polyphenols, fatty acid profile, and mineral composition in the leaves of Eremurus robustus. Proximate composition revealed it is a source of vitamin C, fiber, and protein. Nine phenolic compounds and 24 fatty acids were detected, along with 14 saturated and 10 unsaturated fatty acids using high-performance liquid chromatography (HP-LC) and gas chromatography-mass spectrophotometers (GC-MS). Seventeen mineral compositions were found using an inductively coupled spectrophotometric technique. Vegetable trace metal concentrations can be assessed by comparing the daily diets of plants and humans. The research suggests that Eremurus robustus leaves can serve as nutritional supplements and functional foods, not just for therapeutic uses.
Hydrolyzed Polyphenols from Water Chestnut (Trapa japonica) Suppress Lipase Activity in vitro and Blood Triacylglycerols in Mice.
Yasuda M, Yasutake K, Oikawa D … +2 more , Saiki M, Mitsuta-Takeyama A
J Oleo Sci · 2025 · PMID 40024783 · Publisher ↗
Water chestnut (Trapa japonica) is a thorny aquatic floating plant that contains hydrolyzable polyphenols (WCPs) such as eugeniin, 1,2,3,6-tetra-O-galloyl-β-d-glucopyranose (TGG), and trapain. Previous studies have revea...
Water chestnut (Trapa japonica) is a thorny aquatic floating plant that contains hydrolyzable polyphenols (WCPs) such as eugeniin, 1,2,3,6-tetra-O-galloyl-β-d-glucopyranose (TGG), and trapain. Previous studies have revealed that WCPs have antioxidant properties and can suppress postprandial blood glucose elevation in mice and human participants. However, the functionality of WCPs in treating obesity and metabolic syndrome, which have become significant issues in recent years, remains unclear. Here, we aimed to determine the effects of WCPs on lipid metabolism. We initially investigated the effects of WCPs on pancreatic lipase in vitro. Among WCPs, eugeniin and trapain, which contain a hexahydroxybiphenyl group, particularly inhibited lipase activity. We also found that WCPs suppressed fat accumulation in mouse adipose progenitor 3T3-L1 cells. We compared the effects of WCPs on lipid metabolism among groups of mice (n=7 each) fed for four weeks without (standard control and high-fat (HF) diet controls) or with HFWP 0.5 or HFWP 1 supplemented with 0.5 and 1% WCPs, respectively. Subsequently, we examined lipid-related parameters in adipose tissue, liver, and blood. We found significantly lower plasma triacylglycerol content in the HFWP1 than in the HF group. These findings suggest that WCPs may help prevent the onset of obesity and metabolic syndrome.
Changes in Phytochemical Content and Antioxidant Activities of Soybean Oil during Germination.
Gu M, Kim HW, Kim BH … +4 more , Kim IH, Hyun TK, Lee H, Lee J
J Oleo Sci · 2025 · PMID 40024782 · Publisher ↗
In this study, we investigated the effects of germination on the phytochemical content, antioxidant activity, and oxidative stability of soybean oil. Soybeans were germinated for different periods (0, 2, 4, 6-days). The...
In this study, we investigated the effects of germination on the phytochemical content, antioxidant activity, and oxidative stability of soybean oil. Soybeans were germinated for different periods (0, 2, 4, 6-days). The germinated soybean oil (GSO) contained more α-tocopherol, phytosterols, and carotenoids, while there were no significant differences in fatty acid content. In particular, lutein accumulation was influenced by the modulation of phytoene synthase, lycopene ε-cyclase, and lycopene β-cyclase genes during soybean germination. Antioxidant activities in vitro were the most effective after treatment with 6-day GSO. Moreover, the oxidative stability of GSO was significantly enhanced compared to that of control soybean oil. These results were attributed to the increased content of phytochemicals in soybean oil during germination. This research holds promise not only for the development of functional foods owing to the increased phytochemical content but also for the food industry owing to the enhanced oxidative stability of soybean oil.
Water Resistance Improvement of Sodium Alginate and Black Wolfberry Anthocyanins Based Films Treated by CaCl for Food Packaging.
Li Y, Qiu C, Qi Y … +1 more , Yan Y
J Oleo Sci · 2025 · PMID 40024781 · Publisher ↗
The influence of calcium chloride (2% (w/v), 3% (w/v), 4% (w/v) or 5% (w/v)) as crosslinker on the performance of sodium alginate (SA) and anthocyanin extracted from black wolfberry (BWA) based films was observed. The re...
The influence of calcium chloride (2% (w/v), 3% (w/v), 4% (w/v) or 5% (w/v)) as crosslinker on the performance of sodium alginate (SA) and anthocyanin extracted from black wolfberry (BWA) based films was observed. The results showed that after CaCl treatment, the elongation at break reduced, but the water resistance, thermal stability, and tensile strength of SA/BWA films were significantly improved and they increased with the increase of CaCl concentration. In which, 5%-CaCl treatment endowed the films with optimal performance, which was specifically manifested by a decrease in the swelling ratio from complete dissolution to 163.4% within 60 minutes, water content from 24.9% to 13.1%, water vapor permeation (WVP) from 6.1 g·cm·cm·s·Pa·10 to 1.6 g·cm·cm·s· Pa·10, and an increase in water contact angle from 2.19° to 43.85°, tensile strength from 0.76 MPa to 13.15 MPa. Interestingly, CaCl treatment slightly weakened the antioxidant activity (p < 0.05) but also had around 80% of DPPH radical scavenging rate and improved the visual color change of the film to pH. The films treated by 5%-CaCl monitored the freshness of pork well. Therefore, comparing with traditional SA-based films, the CaCl treated SA/BWA film is a better candidate for active and intelligent packaging application.
Transferrin-modified Gemcitabine Encapsulated Polymeric Nanoparticles Persuaded Apoptosis in U87MG Cells and Improved Drug Availability in Rat Brain: An Active Targeting Strategy for Treatment of Glioma.
Kumar LA, Pattnaik G, Satapathy BS … +5 more , Mohanty DL, Zafar A, Warsi MH, Khalid M, Mujtaba MA
J Oleo Sci · 2025 · PMID 40024780 · Publisher ↗
Among primary brain tumors, glioma has one of the highest fatality rates. Routine chemotherapy often faces off-target drug loss and sub-optimal drug availability at brain tissue. The present study aims at the development...
Among primary brain tumors, glioma has one of the highest fatality rates. Routine chemotherapy often faces off-target drug loss and sub-optimal drug availability at brain tissue. The present study aims at the development of transferrin-conjugated gemcitabine loaded poly (lactic co glycolic acid) nanoparticles (Tf-GB-PLGA-NPs) targeted strategy for brain cancer cell. GB-PLGA-NPs were prepared using solvent evaporation and nanoprecipitation method and then conjugated with Tf. The formulation was characterized for physicochemical parameters, in-vitro release, cytotoxicity, apoptosis (U87MG cell line), and in-vivo pharmacokinetic study. Tf-GB-PLGA-NPs showed 143±6.23 nm of particle size, 0.213 of PDI, -25 mV of zeta potential, and 77.53±1.43% of entrapment efficiency, respectively. Tf-GB-PLGA-NPs exhibited spherical morphology and sustained release of GB (76.54±4.08%) over 24 h. Tf-GB-PLGA-NPs exhibited significant (p < 0.05) cell inhibition against cell line (U87MG) than GB-PLGA-NPs and pure GB. The Tf-GB-PLGA-NPs exhibited higher U87MG apoptosis (61.25%) than GB-PLGA-NPs (31.61%). The Tf-GB-PLGA-NPs exhibited a significantly higher concentration in the brain than pure GB and GB-PLGA-NPs. Tf-GB-PLGA-NPs showed 11.16-fold higher AUC0-t (bioavailability) than pure GB solution and 2.23-fold higher bioavailability than GB-PLGA-NPs. The finding concludes that the Tf-GB-PLGA-NPs are an alternative potent carrier for GB to brain delivery for treating brain cancer.
The Role of Roasting on Changes in Oil Contents, Bioactive Properties, Polyphenol Contents and Fatty Acid Profiles of Turpentine (Pistacia terebinthus L.) Fruit and Oils.
Ahmed IAM, AlJuhaimi F, Özcan MM … +2 more , Uslu N, Karrar E
J Oleo Sci · 2025 · PMID 40024779 · Publisher ↗
In this study, the fluctuations in the oil content, total phenol, total flavonoid, radical scavenging capacity, phenolic constituent profiles and fatty acids of turpentine fruits during roasting of turpentine fruits and...
In this study, the fluctuations in the oil content, total phenol, total flavonoid, radical scavenging capacity, phenolic constituent profiles and fatty acids of turpentine fruits during roasting of turpentine fruits and oils in the oven and microwave treatments were revealed. Total phenolic amounts of turpentine fruit and oils varied between 153.57 (oven) and 197.86 mgGAE/100 g (control) to 17.68 (control) and 30.65 mg GAE/100 g (oven), respectively. Total flavonoid values of the turpentine fruit and oils were characterized to be between 370.36 (microwave) and 567.50 mg/100 g (control) to 89.64 (oven) and 227.50 mg/100 g (microwave), respectively. While quercetin values of the turpentine fruits change between 171.73 (oven) and 330.88 mg/100 g (control), rutin amounts of fruits were defined to be between 3.66 (oven) and 10.00 mg/100 g (control). Catechin amounts of the turpentine fruits roasted in oven and microwave were specified to be between 3.42 (microwave) and 13.69 mg/100 g (oven). Oleic and linoleic acid contents of the oils extracted from raw and roasted turpentine fruits were assessed to be between 50.19 (oven) and 51.30% (control) to 22.89 (control) and 23.39% (oven), respectively. As a result, the phenolic components of turpentine oils were generally higher than those of turpentine fruits.
Discovery and Synthesis of Conjugated Fatty Acids from Natural Products.
Honma T
J Oleo Sci · 2025 · PMID 40024778 · Publisher ↗
Conjugated fatty acids are a promising ingredient for cancer prevention and treatment. Conjugated fatty acids are minor fatty acids that are rarely found in nature, although a wide variety of structures are known. In rec...
Conjugated fatty acids are a promising ingredient for cancer prevention and treatment. Conjugated fatty acids are minor fatty acids that are rarely found in nature, although a wide variety of structures are known. In recent years, studies have been conducted to screen natural products containing conjugated fatty acids and to synthesize conjugated fatty acids using enzymes derived from natural products. As a result, it was found that the seed oils of Centranthus ruber and Valeriana officinalis, which belong to the Valerianaceae family, contain conjugated linolenic acid, which has a conjugated triene structure in the molecule. Furthermore, it was found that parinaric acid, a conjugated tetraenoic fatty acid, can be synthesized by adding α-linolenic acid to enzymes extracted from the brown alga Padina arborescens Holmes. These research results are expected to be useful in securing conjugated fatty acids in quantities that can withstand practical application. Recent studies have reported that the cytotoxic effect of conjugated fatty acids is due to a programmed cell death called "ferroptosis". Many anticancer drugs exhibit anticancer activity through DNA modification, cell cycle arrest, angiogenesis inhibition, and epidermal growth factor receptor inhibition. Conjugated fatty acids, however, induce cell death through a mechanism distinct from these mechanisms and are therefore expected to be effective against cancers resistant to currently used anticancer drugs. The results of these studies will help to promote research on the use of conjugated fatty acids to overcome intractable cancers in the future.
Development of Biomimetic Tactile Sensing Systems for Cosmetics and Cosmetic Ingredients.
Nonomura Y, Saito Y, Nomura S
J Oleo Sci · 2025 · PMID 40024777 · Publisher ↗
The comfortable application of creams and powders and the texture of human skin and hair are essential factors in the design of cosmetics and cosmetic raw materials. However, the mechanisms underlying these diverse and d...
The comfortable application of creams and powders and the texture of human skin and hair are essential factors in the design of cosmetics and cosmetic raw materials. However, the mechanisms underlying these diverse and delicate tactile sensations are poorly understood. We developed a "biomimetic tactile sensing system" to reproduce the interfacial phenomena that occur on the skin surfaces and evaluated the "moist" and "dry" sensations of surface-treated cosmetic powders and the texture of organogel and dispersion formulations. This tactile sensing system consists of a finger model contact probe that mimics the fingerprint and mechanical properties of a human finger and a sinusoidal motion friction evaluation device that can reproduce natural and smooth motions. The finger model contact probe, which mimics the fingerprint and mechanical properties of a human finger, was designed such that the elastic modulus, which reflects hardness, and the surface energy, which affects adhesion, were comparable to those of human skin. In addition, grooves of hundreds of micrometers were engraved to imitate fingerprints. A scotch yoke mechanism that converts elliptical motion into sinusoidal motion was introduced into a sinusoidal motion friction evaluation device to reproduce natural and smooth motions. We analyzed the relationship between sensory evaluation and friction data for cosmetics and cosmetic ingredients and constructed a physical model of tactile sensation evocation. For example, the "moistness" of cosmetic powder was strongly felt when the friction coefficient in the sliding process was low, and a gap existed where the frictional force reached its maximum value. Commercially available makeup cosmetics and sunscreens were characterized based on their friction dynamics and classified accordingly. The wax derived from rice bran and rice paraffin was shown to have high oil-gelling ability, and the resulting gel was smooth to the touch, indicating that it is suitable as a raw material for lipstick and cleansing products.
Medicinal Plants Exhibited Promising Potential to Inhibit Biofilm Formation by Catheter-Associated Bacteria in UTI Patients from Lahore, Pakistan.
Liaqat I, Ibtisam R, Hussain MI … +9 more , Muhammad N, Andleeb S, Naseem S, Ali A, Latif AA, Ali S, Aftab MN, Bibi A, Khalid A
J Oleo Sci · 2025 · PMID 39880641 · Publisher ↗
The current study was designed to evaluate the antibacterial, antibiofilm, and biofilm inhibitory potential of six medicinal plants, including Trachyspermum ammi, Trigonella foenum-graecum, Nigella sativa, Thymus vulgari...
The current study was designed to evaluate the antibacterial, antibiofilm, and biofilm inhibitory potential of six medicinal plants, including Trachyspermum ammi, Trigonella foenum-graecum, Nigella sativa, Thymus vulgaris, Terminalia arjuna, and Ipomoea carneaid against catheter-associated bacteria (CAB). Eighteen CAB were identified up to species level using 16S rRNA gene sequencing, viz., Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa. T. ammi essential oil and T. foenum-graecum methanolic extract combination exhibited the highest antibacterial activity (ZOI; 32.0) against S. aureus. N. sativa essential oil (EO) showed highest ZOI (31.0; p ≤ 0.05) against Proteus mirabilis at 100 µgmL . Among 18 CAB isolated, 13 showed mature biofilm formation on 5 day. All plant extracts demonstrated more than 80% antibiofilm and biofilm inhibition activity. A concentrationdependent increase was observed with plant extracts against CAB during antibacterial, antibiofilm, and biofilm inhibition activities. The study suggests that EO and methanolic extract (ME) of tested plants possess promising antibiofilm and biofilm inhibitory potential against CABs. To our knowledge, this is the first study to report antibacterial, antibiofilm, and biofilm inhibitory potential of T. ammi and N. sativa seed EO, as well as T. foenum-graecum, N. sativa, T. vulgaris, T. arjuna, and I. carnea ME against CAB from medical setting.
Exploring the Fluorescence Bioactive Compounds in the Coelomic Fluid of Earthworms: Insights into Their Structural, Spectroscopic, and Functional Properties for Biomedical Applications.
Venkatachalam S, Christyraj JRSS, Christyraj JDS … +6 more , Subramaniam R, Mathews MGR, Anandharaj JL, Venkatachalam K, Kalimuthu K, Yesudhason BV
J Oleo Sci · 2025 · PMID 39880640 · Publisher ↗
Coelomic fluid of earthworms is a valuable source of novel bioactive compounds with therapeutic applications. To gain insight into the bioactive compounds in the coelomic fluid, this study used Perionyx excavatus, a trop...
Coelomic fluid of earthworms is a valuable source of novel bioactive compounds with therapeutic applications. To gain insight into the bioactive compounds in the coelomic fluid, this study used Perionyx excavatus, a tropical earthworm distinguished for its remarkable ability for regeneration. This study aimed to identify fluorescent bioactive compounds in the coelomic fluid of P. excavatus and to investigate these compounds structural and functional characteristics for potential use in biomedical applications. Fluorescent bioactive compounds present in the coelomic fluid are identified using Thin Layer Chromatography (TLC), UV-visible spectrophotometry, and Spectrofluorometry techniques. Two unknown groups of fluorophore, named CFA and CFB, were analyzed by studying their emission spectra. In addition, GC-MS and LC-MS analyses provides detailed list of bioactive compound present in the coelomic fluid, in which indole and arachidonic acid shown maximum excitation and thus chosen for further studies. Their functional characterization reveals antibacterial activity, cytotoxicity and in-vitro wound healing assays, respectively. Notably, both of them exhibit significant efficacy against Aeromonas hydrophila, Salmonella typhi and Staphylococcus aureus. However, indole shows poor activity against Pseudomonas aeruginosa, whereas arachidonic acid demonstrates effective activity. These findings imply that these bioactive fluorescent compounds may have significant therapeutic applications.
Valorization of Macroalgal Hydrolysate for the Production of Lipids and DHA by Marine Microbes.
Abdel-Wahab MA, Elgorban AM, Bahkali AH
J Oleo Sci · 2025 · PMID 39880639 · Publisher ↗
The present study aimed to explore the potential of macroalgal hydrolysate to serve as an economical substrate for the growth of the oleaginous microbes Aspergillus sp. SY-70, Rhizopus arrhizus SY-71 and Aurantiochytrium...
The present study aimed to explore the potential of macroalgal hydrolysate to serve as an economical substrate for the growth of the oleaginous microbes Aspergillus sp. SY-70, Rhizopus arrhizus SY-71 and Aurantiochytrium sp. YB-05 for lipid and DHA production under laboratory conditions. The macroalgal hydrolysate was used at three concentrations 20, 40 and 80 g/L as a sole carbon source or in combination with 10 g/L of either acetic acid, glycerol, glucose, or sugarcane molasses. Glucose was used as a positive control at four different concentrations: 10, 20, 40, and 80 g/L. Out of the 19 carbon sources tested for the three microbes, 80 g/L macroalgae + 10 g/L molasses was the best source for Aspergillus sp. SY-70 (27.4 g/L DW and 9.73 g/L lipid) and R. arrhizus SY-71 (49.76 g/L DW and 16.88 g/L lipid), whereas 20 g/L macroalgae + 10 g/L glucose afforded the best source for Aurantiochytrium sp. YB-05 (27.93 g/L DW and 11.07 g/L lipid). Among the 19 carbon sources used for the growth of Aurantiochytrium sp. YB-05, we determined the fatty acid profile of the best four carbon sources that gave the highest biomass and lipid percentage. Among the four sources, 20 g/L macroalgal hydrolysate + glucose gave the highest DHA percentage (2.31 g/L), followed by 80 g/L pure glucose (1.68), 80 g/L macroalgal hydrolysate + glycerol (1.64), and 40 g/L macroalgal hydrolysate + molasses (1.52). The three carbon sources can replace pure glucose for the lipid, DPA, and DHA production using Aurantiochytrium sp. YB-05. The results of the current study suggest that we could use macroalgal hydrolysate in combination with molasses or glucose for the production of single-cell oil.
Suppressive Mechanism of Benzalkonium Chloride-Bactericidal Activity in the Presence of Oil.
Ohyagi N, Watanabe Y, Sugita-Konishi Y … +3 more , Yamauchi A, Sato H, Mochizuki M
J Oleo Sci · 2025 · PMID 39880638 · Publisher ↗
Cleaning and sterilization are critical Prerequisite Programs in sanitation management based on HACCP. Most food factories clean and sanitize equipment daily after production using detergents containing benzalkonium chlo...
Cleaning and sterilization are critical Prerequisite Programs in sanitation management based on HACCP. Most food factories clean and sanitize equipment daily after production using detergents containing benzalkonium chloride (BAC). However, in factories that produce oil and fat-rich foods, it has been discovered that microbes can persist on production equipment. Insufficient cleaning protocols may result in secondary contamination of the final products. Unfortunately, there are limited cleaning agents available that are effective in sterilizing microbes in the presence of oil. Moreover, there is a lack of research on the bactericidal mechanisms and bacterial dynamics in oily environments. In this study, we aimed to reduce bacterial contamination on equipment in such factories by hypothesizing that oil diminishes BAC's bactericidal activity. We conducted lab-scale experiments simulating actual factory conditions to examine the effects of oil on BAC's efficacy. Additionally, we investigated the effect of nonionic surfactants, which are known to enhance BAC's bactericidal activity in oil-free conditions, in the presence of oil. The results showed that BAC's bactericidal activity was significantly reduced in the presence of oil. However, the activity was restored by adding an appropriate amount of secondary alcohol ethoxylate (sec-AE). Microscopic observations revealed that bacteria tended to accumulate at the water/oil interface, suggesting that the oil interface might inhibit BAC from effectively attacking the bacteria. The addition of sec-AE appeared to disperse the bacteria into the water layer, thus restoring BAC's bactericidal activity in the presence of oil. These findings are crucial for improving daily cleaning and sterilization processes in food factories operating in high-oil environments to prevent secondary contamination and enhance food safety.
Two-wavelength-selective Soft Actuators Comprising Thermosensitive and Lanthanoid-ion-coordinated Interpenetrating Polymer Network Gels.
Watanabe S, Arikawa K
J Oleo Sci · 2025 · PMID 39880637 · Publisher ↗
Near-infrared wavelength-selective soft actuators have attracted much attention for applications in microsystems in bioliving. It is desirable for the photothermal conversion materials in the actuators to be downsized to...
Near-infrared wavelength-selective soft actuators have attracted much attention for applications in microsystems in bioliving. It is desirable for the photothermal conversion materials in the actuators to be downsized to the molecular scale. However, in conventional actuator materials using copolymer gels composed of thermosensitive and photothermal conversion molecule-coordinated monomers, the strong cross-linking of molecules in the networks impairs the actuator deformation. In this study, we fabricated soft actuators consisting of interpenetrating polymer network (IPN) gels to suppress the cross-linking of the thermosensitive networks. Nd and Yb were used as wavelength-selective photothermal conversion molecules at 808 and 980 nm. Hydrophobic acrylamide derivatives and sodium acrylate were used as the thermosensitive and lanthanoid-ion-coordinated polymers, respectively. The lanthanoid ion concentrations in the IPN gels were about 0.2 M, which is 6 times larger than those of previous gels. The temperature response of swelling degrees (wt%) of the lanthanoid-ion-coordinated IPN gels were three times larger than that of the previous gels. Sandwich structure actuators consisting of Nd and Yb IPN gels bent selectively toward the Nd gel side under 808 nm irradiation and toward the Yb gel side under 980 nm irradiation.
Urea Complexation for Docosahexaenoic Acid Enrichment from Crypthecodinium cohnii Oil: Using Water as the Urea Solvent.
Sun Z, Gong F, Liu M … +9 more , Li Y, Dai Y, Xiao M, Wang J, Yan G, Long L, Huang H, Ni H, Yu L
J Oleo Sci · 2025 · PMID 39880636 · Publisher ↗
Based on the observation that urea, water, and ethyl esters (EE) can form gypsum-like mixtures, this study explored the feasibility of employing water as a solvent for urea in the urea complexation method to enrich n-3 p...
Based on the observation that urea, water, and ethyl esters (EE) can form gypsum-like mixtures, this study explored the feasibility of employing water as a solvent for urea in the urea complexation method to enrich n-3 polyunsaturated fatty acids with docosahexaenoic acid (DHA)-containing ethyl esters (DHA- EE) from Crypthecodinium cohnii as the material. Under the conditions of a urea/DHA-EE ratio of 3, a water/DHA-EE ratio of 0.75, a mixing temperature of 65℃, and a cooling temperature of 20℃, a concentrate containing over 90% DHA was achieved. This demonstrated that using water as a solvent for urea, instead of polar organic solvents, is feasible and efficient for enriching DHA in urea complexation process.
Analysis of Total, Free, and Esterified Sterols and Triterpene Alcohols in Edible Oils using a Gas Chromatography-Flame Ionization Detector.
Tada K, Suganuma Y, Yoshinaga-Kiriake A … +2 more , Koishi S, Yoshinaga K
J Oleo Sci · 2025 · PMID 39880635 · Publisher ↗
Sterols and triterpene alcohols exist in free and esterified forms in edible oils. To date, only few studies have determined the content of free or esterified sterols and triterpene alcohols using gas chromatography-flam...
Sterols and triterpene alcohols exist in free and esterified forms in edible oils. To date, only few studies have determined the content of free or esterified sterols and triterpene alcohols using gas chromatography-flame ionization detection (GC-FID). In this study, analytical conditions were optimized using free and esterified sterol standards. To analyze total sterol and triterpene alcohol (Method A), edible oil sample was saponified in the presence of an internal standard (IS, 5α-cholestan- 3β-ol). However, to analyze free sterol and triterpene alcohol (Method B), the sample was dissolved in hexane/ethyl acetate (90:10, v/v) in the presence of the IS, and the free sterol and triterpene alcohol fractions were isolated via silica gel chromatography. The fractions obtained from Methods A and B were derivatized separately as trimethylsilyl (TMS) ether and analyzed using GC-FID. The esterified sterol and triterpene alcohol contents of edible oil were calculated by subtracting the free sterol content (Method B) from the total content (Method A). The major sterols and triterpene alcohols in canola, soybean, rice bran oil, and lard were separated using a column coated with 5% diphenyl/95% dimethylpolysiloxane. The recovery rates of free sterols spiked into canola oil were 86-106% and 87-109%, while those of esterified sterols (sitosteryl palmitate) spiked into canola oil were 94-105% and －4-2% based on Methods A and B, respectively. The sterol and triterpene alcohol compositions of lard, canola, soybean, and rice bran oils were also determined using Methods A and B, and the results aligned well with those reported in the literature. Altogether, our methods can be successfully used to quantify the levels of sterols and triterpene alcohols in edible oils.
Bioactive Compounds and Fatty Acid Composition of Oils Obtained by Different Extraction Methods from Caper (Capparis ovata Desf.) Seeds.
Ahmed IAM, Akça AE, Juhaimi FA … +3 more , Özcan MM, Uslu N, Karrar E
J Oleo Sci · 2025 · PMID 39880634 · Publisher ↗
In this study, the total phenol, total flavonoid content, antioxidant capacity, phenolic component and fatty acid profiles of caper seed oils extracted by solvent extraction, sonication extraction and cold press methods...
In this study, the total phenol, total flavonoid content, antioxidant capacity, phenolic component and fatty acid profiles of caper seed oils extracted by solvent extraction, sonication extraction and cold press methods were revealed. Total phenol amounts of caper seed oils extracted by cold press, sonication and solvent systems were recorded as 0.10, 0.11 and 0.16 mg GAE/100 g, respectively. There was no statistically significant differences between the total phenol values of caper seed oils provided by sonication and cold press systems (p > 0.05). While the flavonoid amount of the oil extracted from caper seeds by solvent extraction system is determined as 358.9 mg CE/100 g, the total flavonoid amounts of caper seed oils extracted by sonication and cold pressing methods were established as 194.6 and 83.9 mgCE/100 g, respectively. The highest antioxidant capacity was established in the oil provided by solvent extraction (1.456%), followed by ultrasonic extraction (1.453%) and cold press oil (1.448%) in decreasing order. The dominant phenolic components of caper seed oils were quercetin, kaempferol, gallic acid, resveratrol and catechin. The fatty acid detected at the highest value in caper oils extracted by different extraction systems was linoleic acid (61.16-62.74%), followed by oleic, palmitic and stearic acids in decreasing order. Other fatty acids were recorded at low levels. As a result, it can be said that the caper oil extracted by solvent extraction is richer in quercetin and linoleic acid.
Oil Composition and Phenolic Compounds of Plum Seeds Obtained from Fruit Processing By-products Treated by Heat.
Ahmed IAM, Juhaimi FA, Özcan MM … +2 more , Uslu N, Karrar E
J Oleo Sci · 2025 · PMID 39880633 · Publisher ↗
In this study, the effect of microwave drying on oil content, bioactive compounds, antioxidant activity, polyphenols and fatty acid profiles of fresh (control) and dried plum kernels was investigated. The oil quantities...
In this study, the effect of microwave drying on oil content, bioactive compounds, antioxidant activity, polyphenols and fatty acid profiles of fresh (control) and dried plum kernels was investigated. The oil quantities of plum seeds dried were found between 27.40% (control) and 42.42% (900 W). Total phenolic and flavonoid values of fresh (control) and dried-plum seeds were assessed to be between 9.77 (control) and 41.66 mgGAE/100 g (900 w) to 6.90 (control) and 23.67 mg/100 g (900 W), respectively. Total phenol and flavonoid quantities of the plum seeds dried at 900 W were slightly higher than those of the plum seeds dried at 720 W. L* (brightness) values of plum seeds changed between 55.97 and 59.62. Roasting in the microwave oven at 720 W was decreased the L* values of samples, while L* value of sample roasted at 900 W was closed to control. Gallic and 3,4-dihydroxybenzoic acid values of plum kernel samples were assigned to be between 1.19 (720 W) and 2.01 mg/100 g (900 W) to 0.22 (control) and 7.09 mg/100 g (900 W), respectively. Also, catechin and rutin quantities of plum seeds were established between 0.20 (control) and 7.55 mg/100 g (900 W) to 1.42 (control) and 3.59 mg/100 g (900 W), respectively. In general, the amount of phenolic compounds of plum seeds dried at every two watts showed an increase (except quercetin) compared to the control. Only the amount of quercetin decreased partially in the dried samples. While oleic acid quantities of raw (control) and dried plum kernel oils are reported between 68.28% (720 W) and 71.60% (900 W), linoleic acid amounts of plum kernel oils were found between 20.77% (900 W) and 23.49% (720 W). The quantities of saturated fatty acids in plum kernel oils were found to be quite low compared to the content of unsaturated fatty acids.
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