Colloids And Surfaces. B, Biointerfaces[JOURNAL]
Sun
200 papers
Multimodal In-vitro tumour starvation therapy using enzyme-driven piezoelectric nanosystems utilizing ultrasound effect.
Tiwari N, Das R, Kaur M … +2 more , Lall DS, Singh N
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42284959 · Publisher ↗
Several types of tumours and their complexity have been a challenge for experts in medical research, driving the need for innovative approaches. Recent exploration in various nano systems aims to address this issue by ta...
Several types of tumours and their complexity have been a challenge for experts in medical research, driving the need for innovative approaches. Recent exploration in various nano systems aims to address this issue by targeting tumours more precisely, thereby improving treatment efficacy with minimum adverse reactions. In this study, we introduce a glucose-responsive (enzyme powered), piezoelectric nanohybrid platform composed of a core-shell architecture, where Barium Titanate nanoparticles (BTNPs) forms the ultrasound-responsive piezoelectric core, and the mesoporous silica nanoparticles (MSNPs) as the functionalised shell. The shell is engineered with Glucose Oxidase (GOx) enzyme on its surface and loaded with Ethylenediaminetetraacetic acid (EDTA) in the porous structure. These nanosystem (GOx-MS) are designed to target tumour cells in 3D spheroids, with controlled release of EDTA at the target site upon ultrasound (US) application. The GOx-MS functionalization induces localized catalytic glucose consumption, generating asymmetric chemical gradients that enhance nanosystem diffusion within tumour-like environments, which utilizes glucose that is naturally abundant in the tumour microenvironment. This model demonstrated glucose-dependent enhancement in nanosystem mobility and spatial redistribution within 3D tumour spheroids, along with the controlled release of EDTA upon US application. This combination of enzyme-driven motion and US-stimulated drug release displays substrate-dependent enhanced diffusion along with the precise EDTA release, and deeper penetration into the tumour tissue. This study establishes an enzyme-activated, ultrasound-responsive nanohybrid platform that integrates metabolic modulation and externally triggered cargo release for tumour starvation therapy.
Development of bacterial cellulose-based polymeric cryogel scaffold as an eco-friendly biomaterial.
An H, Choi HW, Jang YS … +2 more , Kim YK, Lee MH
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42275805 · Publisher ↗
Cryogels with interconnected macroporous structures are promising three-dimensional scaffolds that facilitate cell infiltration, nutrient transport, and tissue development. This study compared two cryogel systems: gelati...
Cryogels with interconnected macroporous structures are promising three-dimensional scaffolds that facilitate cell infiltration, nutrient transport, and tissue development. This study compared two cryogel systems: gelatin methacryloyl cryogels prepared by free-radical polymerization and oxidized bacterial cellulose-gelatin cryogels synthesized through a green, initiator-free Schiff-base crosslinking method. Both scaffolds exhibited favorable elasticity and biocompatibility, but oxidized bacterial cellulose-gelatin cryogels maintained stable pore structures upon rehydration and showed superior mechanical integrity due to the nanofibrous cellulose framework. Murine preosteoblasts cultured on oxidized bacterial cellulose-gelatin scaffolds exhibited enhanced proliferation and osteogenic differentiation, attributed to optimal pore size and the absence of cytotoxic crosslinking residues. Furthermore, Bovine muscle satellite cells exhibited better spreading and a denser PAX7-positive distribution on oxidized bacterial cellulose-gelatin cryogels, suggesting that the stable porous architecture may support satellite cell maintenance and expansion. These findings demonstrate that tuning pore architecture and using biocompatible crosslinking chemistry significantly improve cryogel scaffold performance. The oxidized bacterial cellulose-gelatin cryogel presents a promising, versatile platform for both hard and soft tissue engineering applications.
An integrated platform for dual-responsive circulating tumor cells release and lipidomics revealing predictive metabolic signatures of breast cancer lung metastasis.
Wang R, Han X, Deng Z … +7 more , Han Q, Wei W, Yin Y, Zhang Y, Xu H, Li Q, Zhang Q
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42275804 · Publisher ↗
BACKGROUND: Breast cancer metastasis remains a leading cause of mortality, involving the dissemination of circulating tumor cells (CTCs) through the bloodstream. Lipid metabolic reprogramming not only fuels cancer cells...
BACKGROUND: Breast cancer metastasis remains a leading cause of mortality, involving the dissemination of circulating tumor cells (CTCs) through the bloodstream. Lipid metabolic reprogramming not only fuels cancer cells but also regulates CTC survival and colonization by coordinating systemic metabolic adaptation across primary tumors, circulation, and target organs. However, the lack of methods for simultaneously isolating viable CTCs and performing multidimensional lipidomic profiling has hindered direct experimental evidence linking CTC dynamics to systemic lipid reprogramming. RESULTS: To address this, an integrated "CTC-multidimensional lipidomics" platform was developed. The system incorporates a dual-responsive nanomaterial (FeO@PEI‑Cys‑HA) with untargeted lipidomics, enabling synchronous high‑fidelity sampling and multidimensional analysis of CTCs, primary tumors, plasma, and lung metastases in a breast cancer lung metastasis model. The nanomaterial achieves > 80% capture efficiency and > 80% high-viability release through hyaluronic acid-mediated targeting and HAase/DTT-triggered dual responses. Integrated lipidomics revealed that CTC dissemination correlates with systemic lipid alterations, with key lipids (e.g., PI 36:2) showing coordinated spatiotemporal patterns. These findings support a three-stage "coordinated lipid reprogramming" framework linking lipid metabolism to CTC shedding, survival, and colonization. SIGNIFICANCE: By enabling synchronized tracking of CTC dynamics and associated lipid alterations, this work provides a novel analytical platform for investigating the CTC-lipid metabolism axis. Beyond serving as a versatile CTC analysis tool, this study highlights the predictive value of lipids in metastasis and offers a new perspective for tumor dissemination research.
On-demand engineering of bimodal nanoimaging agents for monitoring inflammation using quality-by-design.
Vichare R, Srishti SA, Janjic JM
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42275803 · Publisher ↗
Inflammation is a complex immune response to injuries, infections, or tissue damage. Incomplete or dysregulated inflammation resolution accelerates disease progression and impairs functional recovery following trauma-ind...
Inflammation is a complex immune response to injuries, infections, or tissue damage. Incomplete or dysregulated inflammation resolution accelerates disease progression and impairs functional recovery following trauma-induced injuries. Current clinical methods to monitor inflammation rely on quantifying soluble biomarkers, performing biopsies, or using non-specific nuclear imaging to detect downstream inflammation consequences. These techniques lack sensitivity for early detection and provide limited spatiotemporal information. This necessitates the development of a scalable, non-invasive nanoimaging agent capable of monitoring inflammation using clinically available imaging modalities to improve patient prognosis. Given macrophages are the key immune cells recruited at early stages and activated across different inflammatory pathologies, they function as a cellular-level readout that informs the severity of inflammation. In this work, we demonstrate the application of the quality by design (QbD) framework to develop a bimodal imaging nanoemulsion engineered for preferential size-dependent uptake by macrophages and that can be imaged using a near-infrared fluorescence optical imager and a F magnetic resonance imaging system. We integrated face-centered central composite design-based response surface methodology (FCCD-RSM) with desirability function-based optimization to simultaneously achieve multiple critical quality attributes (CQAs) with competing goals. Thus, providing a quantitative framework to anticipate trade-offs in CQAs if factor settings are fine-tuned according to the imaging needs. Furthermore, the presented formulation strategy extended the functional utility of indocyanine green (ICG) beyond short-term imaging procedures (< 24 h) toward multi-day inflammation monitoring using a single low dose. Overall, the study focuses on QbD application for developing a scalable nanoimaging agent and its application for imaging inflammation.
A shape-memory hydrogel with triggered contraction and sustained antibacterial release for acute infected wound healing.
Li J, Qiao Y, Li Z … +2 more , Ling G, Zhang P
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42275802 · Publisher ↗
The healing of acute infected wounds is severely hindered by impaired skin contraction and a dysregulated microenvironment characterized by persistent infection and oxidative stress. Herein, we report a smart shape-memor...
The healing of acute infected wounds is severely hindered by impaired skin contraction and a dysregulated microenvironment characterized by persistent infection and oxidative stress. Herein, we report a smart shape-memory hydrogel (Ag@HSN-AGPS) that integrates two distinct functions to address these challenges: triggered mechanical contraction and sustained antibacterial/antioxidant delivery. The hydrogel matrix is composed of gelatin and polyvinyl alcohol crosslinked by Fe coordination, which locks the material in a temporary stretched shape. Sodium ascorbate is encapsulated in pH-responsive hydrogel beads embedded within the matrix. Upon exposure to the wound microenvironment (pH 7.4), ascorbate is released, reducing Fe to Fe and unlocking the coordination network. This triggers programmable shape recovery, generating a sustained contractile force that actively pulls wound edges together-a bioinspired approach mimicking embryonic wound closure. Hesperetin silver nanoparticles (Ag@HSN NPs) are loaded into an adhesive surface layer composed of dopamine and sodium alginate. These nanoparticles release Ag and hesperetin in a sustained, diffusion-controlled manner, providing continuous antibacterial and antioxidant capacity. The results of in vivo experiments confirmed that Ag@HSN-AGPS hydrogel could promote wound healing and tissue regeneration, indicating its great potential in the treatment of acute infected wounds.
Dynamic palladium speciation governs prodrug activation by nanoparticles in vitro and in vivo.
Latocheski E, Forno GMD, Agnes JP … +4 more , Konč J, Zanotto-Filho A, Bernardes GJL, Domingos JB
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42269251 · Publisher ↗
This work investigates palladium nanocatalysts for C-O bond cleavage of propargyl (Proc) and allyl (Alloc) carbamates of coumarin, rhodamine, and doxorubicin. Catalytic performance was evaluated using colloidal Pd(0) and...
This work investigates palladium nanocatalysts for C-O bond cleavage of propargyl (Proc) and allyl (Alloc) carbamates of coumarin, rhodamine, and doxorubicin. Catalytic performance was evaluated using colloidal Pd(0) and Pd(II) nanoparticles, across buffered media, cell cultures, and mouse models. In aqueous buffers, Pd(II)-NPs showed higher activity toward Proc substrates, while Alloc substrates remained largely unreactive. In cellular environments, however, both nanoparticle systems promoted efficient uncaging of Alloc and Proc derivatives, consistent with dynamic palladium speciation under biological relevant conditions. Among the system evaluated, the Pd(0)-NPs/Alloc-DOX combination elicited the strongest reduction in cell viability while maintaining low intrinsic toxicity, thus emerging as the most robust platform for in vivo evaluation. In murine tumor models, Pd(0)-NPs combined with Alloc-DOX significantly reduced tumor growth compared to controls and individual treatments. In contrast, Proc-DOX displayed comparable antitumor effects in the presence and absence of palladium, suggesting partial activation through endogenous pathways. These findings highlight that catalytic outcomes are governed not only by palladium oxidation state but also by dynamic speciation and biological microenvironment.
Bacterial cuproptosis-like death: A novel antibacterial strategy based on copper nanomaterials.
Luo C, Li Y, Liu H … +1 more , Huang K
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42269250 · Publisher ↗
As the crisis of antimicrobial resistance continues to escalate, there is a growing need for therapeutic approaches that move beyond traditional antibiotic-based paradigms. Bacterial cuproptosis-like death (BCLD) has eme...
As the crisis of antimicrobial resistance continues to escalate, there is a growing need for therapeutic approaches that move beyond traditional antibiotic-based paradigms. Bacterial cuproptosis-like death (BCLD) has emerged as a promising concept that leverages intracellular copper overload to trigger cell death. This review elucidates the mechanism of BCLD, which is characterized by a metabolic catastrophe proposed to involve the disruption of lipoylated tricarboxylic acid cycle enzymes and the disintegration of iron-sulfur clusters, ultimately leading to irreversible metabolic collapse and bacterial cell death. The review also details the rational design of copper-based nanomaterials that exploit this vulnerability. A progressive design philosophy is outlined, ranging from basic nanocarriers to systems enabling spatiotemporally controlled delivery, and further to strategies that potentiate BCLD through metabolic sensitization or multimodal synergy. The applications of these engineered platforms are evaluated in challenging infection models, including chronic wounds and implant-associated biofilms, highlighting their combined antibacterial, anti-inflammatory, and tissue-regenerative potential. Finally, we critically discuss key translational challenges, such as the lack of standardized metrics for BCLD quantification, incomplete mechanistic understanding of the core pathway, long-term biosafety concerns, and the bottleneck in scalable material manufacturing. Interdisciplinary research directions are proposed to advance BCLD toward a clinically viable antibacterial strategy.
Processing-defined organization governs plasmonic response and stability in lipid-coated gold nanoparticles.
Çi̇l C, Kılıç A
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42259177 · Publisher ↗
Lipid-coated gold nanoparticles (LC-AuNPs) are widely explored as plasmonic nano-bio interfaces that combine membrane-mimetic surface organization with optical functionality. However, modest LSPR red-shifts and apparent...
Lipid-coated gold nanoparticles (LC-AuNPs) are widely explored as plasmonic nano-bio interfaces that combine membrane-mimetic surface organization with optical functionality. However, modest LSPR red-shifts and apparent salt stability are often treated as indicators of conformal bilayer formation, although population-averaged optical and colloidal measurements do not uniquely resolve nanoscale interfacial organization. Here, we compared sonication- and extrusion-based coating routes to determine how processing history governs LC-AuNP organization, plasmonic response, and colloidal stability. Hydrodynamic sizing, zeta potential analysis, spectral line-shape descriptors, PBS stability assays, electron microscopy, and semi-empirical LSPR response modeling were integrated into a unified characterization framework. Sonication-driven coating supported lipid association but produced heterogeneous populations with condition-dependent hydrodynamic sizes, reaching > 400 nm under prolonged conditions, and modest LSPR red-shifts (Δλ ≈ 2 nm) that did not uniquely resolve interparticle organization. In contrast, mechanically constrained extrusion (11 ×-13 × passes) reduced coupling-associated spectral heterogeneity, yielding optically coherent populations converging toward ∼200 nm and typically accompanied by small but reproducible blue-shifts (Δλ ≈ 0.4-1.6 nm). Incorporation of DSPE-PEG further reduced population dispersion and improved optical stability under physiological ionic strength, consistent with steric regulation of interparticle organization.Electron microscopy of DSPE-PEG-containing multicomponent formulations revealed lipid-associated layers with an apparent thickness of ∼4.6 nm, compatible with bilayer-scale organization but not diagnostic of conformal coverage across the entire population. LSPR response modeling supported a shift-direction-resolved interpretation of ensemble spectra, in which positive-shift responses were evaluated for effective dielectric/coupling contributions, whereas blue-shifted states were assessed using a physically constrained required-decoupling framework. Together, these results show that LC-AuNP plasmonic peak shifts are governed by fabrication-defined interparticle organization in addition to lipid association. The absence of a red-shift therefore does not imply the absence of lipid coating. Rather, LSPR peak position should be interpreted as an ensemble-level descriptor of processing-defined organization rather than as a direct reporter of conformal bilayer formation.
Antimicrobial activity of bis(4-carboxyanilinium) selenate and its interaction with synthetic liposomes: A biophysical, molecular docking and molecular dynamics study.
Panicker L, Tripathi P
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42259176 · Publisher ↗
Bis(4-carboxyanilinium) selenate (B4-CASeO₄) was investigated as a membrane-active antimicrobial agent using microbiological, biophysical, and computational approaches. The compound exhibits broad-spectrum antimicrobial...
Bis(4-carboxyanilinium) selenate (B4-CASeO₄) was investigated as a membrane-active antimicrobial agent using microbiological, biophysical, and computational approaches. The compound exhibits broad-spectrum antimicrobial activity, with minimum inhibitory concentrations (MICs) of 2.63 mg mL⁻¹ against Escherichia coli, Bacillus subtilis, and Staphylococcus carnosus, and 1.31 mg mL⁻¹ against Saccharomyces cerevisiae. The minimum bactericidal concentration (MBC) values were comparable to the corresponding MIC values, consistent with a bactericidal mode of action. Biophysical studies using DPPC and DPPE model membranes reveal concentration- and time-dependent membrane perturbation, characterized by increased phase transition temperature, altered transition enthalpy, vesicle size growth, and a significant increase in zeta potential, indicating enhanced bilayer ordering and interfacial reorganization. Fluorescence measurements further indicate increased exposure of hydrophobic regions upon interaction with B4-CASeO₄. Molecular dynamics simulations show that 4-carboxyanilinium cations preferentially localize at the lipid-water interface, inducing bilayer ordering without deep penetration, while selenate ions remain largely solvated. Additional simulations performed with the physiologically relevant anionic form of 4-aminobenzoic acid (4-ABA) showed predominantly interfacial localization with weak transient membrane interactions. Docking studies suggest favorable binding of the cationic component to key microbial enzymes, supporting a dual mechanism involving membrane modulation and enzyme inhibition. Comparative analysis with 4-aminobenzoic acid confirms the enhanced efficacy of B4-CASeO₄. These findings establish B4-CASeO₄ as a promising membrane-active antimicrobial agent.
Construction of colorimetric and fluorescence dual-mode sensing platform based on FeSnO(OH)/Pt NPs and CsPbBr@DMSNs for sensitive detection of TBHQ.
Wei Z, Wang H, Lv Y … +2 more , Liang Q, Su X
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42259175 · Publisher ↗
Tert-butylhydroquinone (TBHQ) as a cost-effective synthetic and efficient antioxidant is extensively added to food products, excessive intake of TBHQ is associated with potential health risks. Herein, a colorimetric and...
Tert-butylhydroquinone (TBHQ) as a cost-effective synthetic and efficient antioxidant is extensively added to food products, excessive intake of TBHQ is associated with potential health risks. Herein, a colorimetric and fluorometric detection method integrating FeSnO(OH)/Pt nanoparticles (Pt NPs) and CsPbBr@DMSNs for the sensitive detection of TBHQ was developed. CsPbBr@DMSNs nanocomposites were prepared by embedding CsPbBr nanocrystals (NCs) within dendritic mesoporous silica nanoparticles (DMSNs), resulting in excellent aqueous stability and enhanced luminescence properties. Pt NPs were in situ grown on FeSnO(OH) nanocubes with a favorable specific surface area by a coprecipitation method, yielding the FeSnO(OH)/Pt NPs with excellent peroxidase-like activity. 3,3',5,5'-tetramethylbenzidine (TMB) could be catalyzed by FeSnO(OH)/Pt NPs to produce oxTMB, thereby quenching the fluorescence of CsPbBr@DMSNs by inner filter effect. TBHQ has potent antioxidant properties and could prevent TMB from being oxidized, thereby recovering the fluorescence of CsPbBr@DMSNs. Hence, a colorimetric and fluorescence dual-signal detection system for TBHQ detection was developed, with LODs of 3.52 µM and 2.12 µM, respectively. Moreover, this sensing platform was successfully used for the TBHQ determination in edible oils, indicating good practicability.
Bioinspired DNA sponge interface coupled with deoxyribozyme autography for nanoparticle-free nucleic acid detection on a strip.
Cao X, Yu Q, Zhu X … +4 more , Ren Z, Xu Q, Yu F, Xu Y
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42251833 · Publisher ↗
Conventional nanoparticle-labeled lateral flow assays often face limitations such as aggregation in incompatible buffers, complex preparation procedures, and high costs. Here, we present a nanolabel-free lateral flow chr...
Conventional nanoparticle-labeled lateral flow assays often face limitations such as aggregation in incompatible buffers, complex preparation procedures, and high costs. Here, we present a nanolabel-free lateral flow chromatographic platform that integrates lossless strand displacement amplification of deoxyribozyme with a barcoded DNA sponge-based capture interface. In this design, functional G-quadruplex structures serve as intrinsic reporting units instead of conventional nanoparticles, generating catalytic or fluorescent signals upon ligand binding. The DNA sponge network functions as a high-capacity capture interface on the test line. Its porous and hydrophilic architecture enhances probe loading and locally retains the flowing solution, thereby prolonging the interaction between amplification products and immobilized multivalent probes. This structural confinement improves the capture efficiency of nucleic acid amplicons and effectively suppresses the coffee-ring effect during the fabrication of the capture interface, ensuring high probe density within a small sensing zone, uniform probe distribution, and reproducible signal readout. For microRNA detection, the proposed platform achieves a detection limit as low as 10 fM and demonstrates the capability for single-base mismatch discrimination. This work presents an interface-engineering strategy that enables label-free signal generation and robust probe immobilization, broadening the integration of nucleic acid amplification with strip-based biosensing for advanced biomedical analysis.
Copper-coordinated fungal chitosan sutures with antibacterial and pro-healing performance.
Li Y, Zhao X, Dou Y … +7 more , Wang S, Gao H, Cui A, Cheng G, Li J, Wang B, Li L
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42250335 · Publisher ↗
Fungal-derived chitosan (FCS) has emerged as a sustainable alternative to animal-derived chitosan (ACS) for biomedical applications. In this study, FCS fibers were fabricated via wet spinning and systematically compared...
Fungal-derived chitosan (FCS) has emerged as a sustainable alternative to animal-derived chitosan (ACS) for biomedical applications. In this study, FCS fibers were fabricated via wet spinning and systematically compared with ACS fibers. FCS fibers exhibited a smaller fiber diameter, slightly lower crystallinity, and higher elongation, while maintaining a comparable chemical structure and thermal stability to ACS fibers. FCS fibers were subsequently twisted into yarns and functionalized with copper ions (Cu) to prepare antibacterial sutures (Cu@FCS). Cu loading onto FCS yarns increased with copper solution concentration, reaching 9.32 ± 4.01‰ (Cu-L@FCS), 30.83 ± 3.43‰ (Cu-M@FCS), and 126.25 ± 21.11‰ (Cu-H@FCS). Importantly, both Cu-L@FCS and Cu-M@FCS exhibited excellent cytocompatibility and hemocompatibility, whereas Cu-H@FCS showed cytotoxicity. Both Cu-L@FCS and Cu-M@FCS demonstrated potent antibacterial activity against S. aureus and E. coli. These groups also significantly enhanced L929 cell migration, achieving wound closure rates of 95.17 ± 2.78% and 96.97 ± 1.93% at 24 h. In vivo, Cu-M@FCS sutures effectively reduced inflammatory cell infiltration and promoted the healing of infected wounds. Collectively, Cu@FCS sutures combine sustainability, robust antibacterial activity, and enhanced wound-healing performance, underscoring their considerable promise for clinical translation in biomedical applications.
Surface wettability regulation effect ofceramic membrane on bovine milk casein-whey protein separation.
Ding G, Qi M, Zhang Q … +7 more , Si Z, Zhang C, Yao S, Wu H, Chen L, Liu Q, Qin P
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42247959 · Publisher ↗
In the dairy industry, efficient separation of casein (CN) and whey protein is crucial for products like infant formula and sports nutrition. Membrane separation technology, particularly ceramic membranes, offers advanta...
In the dairy industry, efficient separation of casein (CN) and whey protein is crucial for products like infant formula and sports nutrition. Membrane separation technology, particularly ceramic membranes, offers advantages including high temperature resistance, mechanical strength, and chemical stability. Surface wettability, as a key interfacial parameter, directly reflects membrane-protein interactions and influences flux, separation efficiency, and fouling. In-depth exploration of the intrinsic connection between the surface wettability of ceramic membrane and the separation effect of proteins is conducive to the precise control of the protein separation process, effectively reducing the risk of membrane fouling, increasing membrane flux, and thereby significantly improving the efficiency of protein separation. This work systematically investigates the effects of membrane pore size, operating pressure, and temperature process parameters on the separation effect and membrane flux of CN and whey protein, and combines surface wettability for mechanism analysis to reveal the migration rules and separation mechanisms of protein molecules on ceramic membrane surface. The results will provide theoretical basis and technical support for the practical application of ceramic membrane technology in the dairy industry, promoting the green and intelligent development of dairy product processing technology.
Application of graphyne nanozymes in biosensing and biomedicine.
Gao M, Ding J, Liu B … +2 more , Zhao H, Ye D
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42247958 · Publisher ↗
Nanozymes have emerged as powerful alternatives to natural enzymes due to high stability, low cost, and tunable activity. Among numerous nanozyme materials, graphyne, an emerging two-dimensional carbon allotropic form, d...
Nanozymes have emerged as powerful alternatives to natural enzymes due to high stability, low cost, and tunable activity. Among numerous nanozyme materials, graphyne, an emerging two-dimensional carbon allotropic form, demonstrates immense application potential in the field of nanozymes. This is attributed to its unique sp-sp hybridized carbon structure, abundant large π-conjugated systems, uniform pore architecture, and outstanding chemical stability. The tunable electronic structure and surface chemical properties of graphyne enable it to mimic the activities of various natural enzymes, providing a novel technical platform for biosensing and biomedicine. In biosensing, graphyne nanozymes are widely utilized for constructing highly sensitive and selective biosensors due to their exceptional catalytic efficiency and stability. The enzyme-like activity of graphyne nanozymes catalyzes substrates to produce colorimetric, fluorescent, or chemiluminescent signals, enabling sensitive detection of target molecules. Consequently, they find extensive applications in biomolecular detection, disease diagnosis, and environmental pollutant monitoring. In biomedicine, the multi-enzymatic activity of graphyne nanozymes has opened new avenues for their application in disease treatment, particularly in tumor therapy. Simultaneously, the Fenton-like reaction modulates reactive oxygen species, regulating oxidative stress balance. This multi-enzyme synergistic effect efficiently induces tumor cell apoptosis. Moreover, the unique pore structure serves as an ideal carrier for drug molecules, enabling targeted delivery and controlled release of chemotherapeutic agents. Combined with its inherent photothermal conversion capability, this approach achieves synergistically enhanced tumor therapy. Graphyne-based nanozymes, as a new generation of high-performance nanozymes, will play a pivotal role in future precision medicine, smart sensing, and biotechnology innovation.
A microenvironment-responsive hydrogel biointerface for targeted inhibition of RIPK1-dependent programmed cell death in diabetic wound healing.
Xie F, Liu H, Li Y … +8 more , Yang X, Chang H, Fu R, Xiang H, Liu X, Tang W, Tan L, Xie Y
Colloids Surf B Biointerfaces · 2026 May · PMID 42247957 · Publisher ↗
Diabetic chronic nonhealing wounds are characterized by persistent inflammation, oxidative stress, and impaired tissue regeneration, which are closely associated with receptor-interacting serine/threonine-protein kinase...
Diabetic chronic nonhealing wounds are characterized by persistent inflammation, oxidative stress, and impaired tissue regeneration, which are closely associated with receptor-interacting serine/threonine-protein kinase 1 (RIPK1)-mediated programmed cell death. Although RIPK1 kinase represents a promising therapeutic target, its role in diabetic wound healing and effective local delivery strategies remain largely unexplored. Here, we report a local therapeutic system for diabetic wound healing, RIPK1i@PTM/TA, based on a pH/glucose dual-responsive hydrogel biointerface rationally designed to adapt to the pathological wound microenvironment. The hydrogel is constructed from a poly(acrylic acid)-terminal-olefin polyethylene glycol ether-phenylboronic acid copolymer (PTM) and dynamically crosslinked with tannic acid (TA), endowing the network with microenvironment-sensitive structural modulation, tissue adhesion, and antibacterial functionality. RIPK1 inhibitor is stably immobilized within the reversible crosslinked matrix, enabling localized, on-demand, and sustained drug release at the wound-material interface in response to acidic pH and elevated glucose levels. Functionally, RIPK1i@PTM/TA enhanced fibroblast activity in vitro, and in diabetic chronic wound mouse models effectively suppressed RIPK1-dependent cell death, alleviated inflammation and oxidative stress, and promoted angiogenesis, ultimately accelerating wound closure and structural reconstruction. Collectively, this study demonstrates an effective microenvironment-responsive hydrogel for topical drug delivery in refractory diabetic wounds.
Hyaluronic acid-camouflaged dendritic silica nanoparticles enable targeted cuproptosis and photothermal therapy for lung cancer.
Li X, Li C, Liu D … +1 more , Wang Y
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42247956 · Publisher ↗
Cuproptosis, has attracted increasing attention in cancer therapy due to its apoptosis-independent mechanism and potential to overcome drug resistance. However, current copper-mediated nanotherapeutics are still limited...
Cuproptosis, has attracted increasing attention in cancer therapy due to its apoptosis-independent mechanism and potential to overcome drug resistance. However, current copper-mediated nanotherapeutics are still limited by low drug-loading efficiency and insufficient therapeutic versatility. Herein, a tumor microenvironment-responsive nanoplatform, HA-SiO@ESCu-Hemin (SNECHA), was developed for combined cuproptosis-photothermal therapy. Dendritic SiO nanoparticles were sequentially loaded with elesclomol-copper complex (ESCu) and hemin, followed by hyaluronic acid (HA) coating to achieve CD44-mediated tumor targeting and HAase/pH-responsive drug release. After internalization, ESCu induced cuproptosis through FDX1-mediated copper reduction, Fe-S cluster disruption, and DLAT-associated proteotoxic stress, while hemin enabled 808 nm laser-triggered photothermal therapy. SNECHA showed efficient near-infrared photothermal conversion with an efficiency of 46.69% and increased to 57.3 °C. In addition, it exhibited enhanced cellular uptake, selective cytotoxicity toward A549 cells with IC value of 103.2 nM under laser illumination, and significantly improved antitumor efficacy. In vivo results further demonstrated enhanced tumor accumulation, superior tumor growth inhibition, and favorable preliminary biosafety. This work provides a targeted nanotherapeutic strategy for enhanced cancer treatment through cuproptosis-photothermal combination therapy.
Bacterial Pickering emulsion stability quantified using microfluidic droplet coalescence tests.
Li X, Takahashi K, Obana N … +2 more , Dreyfus R, Utada AS
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42247955 · Publisher ↗
HYPOTHESIS: Pickering emulsions are typically stabilized with passive colloids. By replacing these passive agents with active bacteria, Pickering emulsion functionality can be enhanced; however, the complexity of the bac...
HYPOTHESIS: Pickering emulsions are typically stabilized with passive colloids. By replacing these passive agents with active bacteria, Pickering emulsion functionality can be enhanced; however, the complexity of the bacterial surface, which depends on surface-expressed molecules, presents major challenges to their utilization. To develop a framework for understanding bacterial-interface interactions, we combine dynamic microfluidic droplet-stability tests with a theoretical model of cell-interface interaction potentials. EXPERIMENTS: We describe a microfluidic assay to evaluate bacterial stabilization of droplets against coalescence, testing several species and mutants over a range of cell concentrations. We characterize the zeta-potential and three-phase contact angle of each strain to use as inputs for an extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) model, which we use to interpret our emulsion stability and cell coverage results. FINDINGS: Our microfluidic assay reveals two distinct patterns of emulsion stabilization. In one, emulsion stability increases as cell concentration increases, consistent with classical Pickering emulsion stabilization. In the other, we observe an unexpected decrease in stability with increasing cell concentration for bacteria with larger negative charge and lower hydrophobicity. We propose a mechanism that these bacteria electrostatically stabilize droplets at low concentrations while promoting coalescence at high concentrations through a droplet-bridging mechanism. In addition to our XDLVO model, we further extend our analysis to account for the influence of bacterial appendages, which we show play a dominant role in interface breaching. Our model and results demonstrate the potential for developing a framework for evaluating bacteria to use as Pickering emulsion stabilizers.
Light-responsive polyphosphoester nanocarriers for cascade-amplified synergistic photodynamic-chemotherapy of colon cancer.
Guo W, Zheng Y, Xie J … +5 more , Hao M, Ge Q, Yin T, Cao J, Cao Z
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42247954 · Publisher ↗
Early-stage colorectal cancer is often asymptomatic, many patients are diagnosed at advanced stages (III-IV) and require systemic therapy, which is frequently compromised by nonspecific biodistribution and severe systemi...
Early-stage colorectal cancer is often asymptomatic, many patients are diagnosed at advanced stages (III-IV) and require systemic therapy, which is frequently compromised by nonspecific biodistribution and severe systemic toxicity. To address these clinical challenges, we developed a biocompatible poly (ethylene glycol)-polyphosphoester (PEG-b-PPE) block copolymer-based nanocarrier for the co-delivery of the photosensitizer chlorin e6 (Ce6) and chemotherapeutic drugs, obtaining a drug-loaded nanomedicine termed S-PCD. Following intravenous administration, S-PCD passively accumulated in tumor, and the encapsulated Ce6 could generate reactive oxygen species (ROS) upon near-infrared light irradiation. The produced ROS further induced an oxidative hydrophilic-hydrophobic transition of the polyphosphoester backbone. This process rapidly destabilized the nanoparticles and triggers the release of the encapsulated chemotherapeutic agents. As a result, the S-PCD system enables an ROS-triggered cascade process leading to synergistic photodynamic-chemotherapy. In vivo studies demonstrated that S-PCD enabled efficient delivery of broad-spectrum anticancer drugs, such as doxorubicin and oxaliplatin, resulting in effective tumor growth inhibition in a CT26 colorectal cancer model. These results indicated that the ROS-responsive polyphosphoester-based nanoplatform represented a versatile and scalable strategy for systemic chemotherapy in colorectal cancer.
Magnetic resonance imaging-guided thermoradiotherapy for glioblastoma using Gd-loaded melanin nanoparticles reverses anoikis resistance through the ITGA5-PI3K/AKT axis.
Shi Y, Wang W, An H … +6 more , Nan D, Lian J, Zhang X, Peng J, Yang X, Liu P
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42247953 · Publisher ↗
Glioblastoma (GBM) remains a highly aggressive brain tumor with limited therapeutic outcomes owing to therapy resistance, particularly resistance to anoikis, which prompts researchers to seek novel treatments for GBM. To...
Glioblastoma (GBM) remains a highly aggressive brain tumor with limited therapeutic outcomes owing to therapy resistance, particularly resistance to anoikis, which prompts researchers to seek novel treatments for GBM. To address this challenge, we developed a theranostic nanoplatform, Gd-loaded PEGylated melanin nanoparticles (Gd-MNP-PEGs), to achieve magnetic resonance imaging (MRI)-guided thermoradiotherapy (the combination of photothermal therapy and radiotherapy). This biocompatible nanoplatform enables high-resolution MR imaging for tumor boundary delineation and synergistically combines photothermal therapy with radiotherapy to overcome anoikis resistance. Transcriptomic analysis further identified integrin alpha-5 as a key therapeutic target, acting through the PI3K/AKT signaling pathway. By integrating diagnostic imaging with a mechanism-based therapeutic strategy, this work presents a promising and innovative approach to improve GBM treatment outcomes with minimal side effects.
Remote control of protein deposition on magnetoactive composites.
Rodriguez-Lejarraga P, Maestu JF, Petrenko V … +2 more , Lanceros-Mendez S, Silván U
Colloids Surf B Biointerfaces · 2026 Jun · PMID 42241889 · Publisher ↗
Upon biomaterial implantation into the body, its surface is coated by proteins in a process known as competitive adsorption. It is known that the physicochemical properties of the surface strongly impact the binding kine...
Upon biomaterial implantation into the body, its surface is coated by proteins in a process known as competitive adsorption. It is known that the physicochemical properties of the surface strongly impact the binding kinetics and structural properties of the adsorbed protein layer, and consequently the biological activity of the material. For instance, hydrophobicity causes significant protein conformational changes that provoke mechano-insensitivity of mesenchymal stem cells (MSCs), while polyethylene glycol (PEG) functionalization repels protein adsorption, thereby resulting in "non-fouling" surfaces to which cells cannot adhere. In the current work, we present a magnetoactive composite material composed of a piezoelectric polyvinylidene fluoride matrix with embedded CoFeO nanoparticles (PVDF-CFO) that enables the modulation of fibronectin and collagen type I adsorption using externally applied magnetic fields. Specifically, we demonstrate that exposure to alternating magnetic fields significantly impacts the supramolecular organization of the fibronectin layer and the compaction of collagen fibrils deposited on the composite films.
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