Berliner Und Munchener Tierarztliche Wochenschrift[JOURNAL]
Sun
200 papers
Isolation and antimicrobial susceptibility of Streptococcus castoreus isolated from carcasses of European beavers (Castor fiber) in Germany.
Schulze C, Kutzer P, Winterhoff N … +3 more , Engelhardt A, Bilk S, Teubner J
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26591385
Streptococcus (S.) castoreus was isolated from seven beavers (Castor fiber), which were found dead in the federal state of Brandenburg, Germany, and submitted to the Berlin-Brandenburg State laboratory for post mortem di...
Streptococcus (S.) castoreus was isolated from seven beavers (Castor fiber), which were found dead in the federal state of Brandenburg, Germany, and submitted to the Berlin-Brandenburg State laboratory for post mortem diagnostics. The isolates originated from various inflammatory processes where they were associated with other Gram negative and Gram positive aerobic and/or anaerobic bacteria (i. e. Actinobacillus sp., species of the Actinomycetaceae family, coliform bacteria, Fusobacterium sp., Prevotella sp.), but also from cloacal swabs. Testing their antimicrobial susceptibility, all S. castoreus isolates and the type strain S. castoreus (DSM 17536) were classified as being susceptible to penicillin G, ampicillin, amoxicillin-clavulanic acid, 1st generation cephalosporins, erythromycin, tetracycline, and chloramphenicole but intermediate to gentamicin. Considering both the polymicrobial flora isolated from the inflamed tissues and the fact that antimicrobials of a narrow spectrum (namely penicillins, 1st generation cephalosporins, macrolids and lincosamids) can be toxic to rodents, chloramphenicol might be a suitable drug for treatment of beavers suffering from S. castoreus (mixed)-bacterial infections.
[Causes, diagnostics and course of disease in 194 cats with anemia].
Merten N, Weingart C, Kohn B
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26591384
Anemia is a common hematological alteration in cats. The objective of this study was to evaluate the frequency of different types of anemia and the course of disease in cats with a hematocrit (hct) < 0.26 l/l. In a perio...
Anemia is a common hematological alteration in cats. The objective of this study was to evaluate the frequency of different types of anemia and the course of disease in cats with a hematocrit (hct) < 0.26 l/l. In a period of 18 months 194 cats were included and assigned to different anemia groups based on history, physical examination and laboratory parameters. Most cats had acute blood loss anemia (BA; 75/194; 38.7%). Frequent causes were trauma (39/75), hematuria (13/75) and hemostatic disorders (9/75). Anemia of inflammatory and neoplastic disease (AID) occurred in 22.2% (43/194) and hemolytic anemia (HA) in 18% (35/194). Half of those were presumptively immune-mediated (IHA). Four cats were diagnosed with hemotropic mycoplasma infection. Rare causes of anemia included anemia of renal disease (ARD; 18/194; 9.3%) and intramedullary non-regenerative anemia (INR; 13/194; 6.7%). The latter either had retroviral infection (6/13) or neoplasia (6/13). In cats with HA and INR anemia was often severe and very severe (Hct < 0.14 l/l) and in cats with AID and ARD usually mild (Hct 0.20-0.25 l/l). Cats with BA had significantly lower total protein concentrations than those with INR (p = 0,001), HA, AID and CNE (p < 0,001) and those with HA most often had hyperbilirubinemia (21/27). Blood transfusions were primarily given to cats with BA (37/75) and HA (19/35), especially those with IHA (13/17). 69% of the patients survived the first 14 days after the anemia was detected for the first time. Cats with HA had the highest survival rate.
[Evaluation of an immunochromatographic dipstick test for the assessment of tetanus immunity in horses].
Recknagel S, Snyder A, Blanke A … +3 more , Uhlig A, Brüser B, Schusser GF
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26591383
Knowledge of tetanus immunity in equine patients is crucial in cases of injuries, elective surgeries, or when effective vaccination protocols are to be designed. The Fassisi® TetaCheck is a stall-side rapid test which wa...
Knowledge of tetanus immunity in equine patients is crucial in cases of injuries, elective surgeries, or when effective vaccination protocols are to be designed. The Fassisi® TetaCheck is a stall-side rapid test which was developed to address these issues. The purpose of the present study was to evaluate its performance parameters. To this end, the qualitative test results obtained by two blinded observers were compared to tetanus toxoid antibody levels from 99 serum samples, measured with a double antigen ELISA. Additionally the colour intensities of the test window were quantified using a camera and photo editing software. Assuming that the protective level of tetanus toxoid antibodies is ≥ 0.1 IE/ml, the tetanus quick stick (TQS) showed a sensitivity of 83.6% and a specificity of 100%. almost perfect (K = 0.88). Exchanging the observer did not affect the interpretation of theTQS (K = 0.80; K = 0.84). The definition of five distinct colour intensities of the "test window" enabled a clear differentiation of unprotected individuals from those with a protective immunity. There was a linear relationship between the objectively measured colour intensities and the tetanus toxoid antibody concentration (r2 = 0.74). The TQS thus proved to be a robust and reliable test in the stall-side assessment of tetanus immunity in horses. Its implementation in equine daily practice can help to avoid unnecessary immunizations in adult horses and therefore minimize vaccination side effects.
Associations between Mycobacterium paratuberculosis sero-status, milk quality parameters, and reproduction in dairy cows.
Pesqueira MN, Factor C, Mato I … +7 more , Sanjuán ML, Macias L, Eiras C, Arnaiz I, Camino F, Yus E, Diéguez FJ
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26591382
The objective of this study was to investigate the influence of Mycobocterium avium subsp. paratuberculosis (MAP) sero-status of dairy cows on different milk production variables and reproductive traits. The study was ca...
The objective of this study was to investigate the influence of Mycobocterium avium subsp. paratuberculosis (MAP) sero-status of dairy cows on different milk production variables and reproductive traits. The study was carried out on 40 herds from the region of Galicia (North-West Spain). These herds were randomly selected from a larger group that had taken part in a voluntary paratuberculosis control program since 2005, which involves regular serum sampling of every adult animal to run antibody-ELISA tests. Milk production and reproductive data were obtained from the "Dairy Herd Improvement Program (DHIP) of Galicia". All the gathered data were processed following a linear regression model. Results indicated that there was no significant effect of MAP sero-status on individual milk production variables. However, a significant difference was observed at the calving-to-first-insemination interval, with an average increase of 14 days in positive animals compared to negatives. It has to be taken into consideration that the paratuberculosis status was only defined by the serological status. Since para tb-infected animals may have antbodies or may not, para tb-positive animals can also be included in the sero-negative group of animals, which may bias the results.
[Treatment of Tritrichomonas foetus in a cat colony with delayed release ronidazole tablets in the small intestine].
Reinert J, Gerold S, Scheurlen U … +3 more , Daniels R, Wolm M, Iglauer F
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26591381
Seven abyssinian cats (two male, five female) showed intermittent green-yellow mucous diarrhoea, sometimes an inflammation of the anal region and faecal incontinence even after long-time treatment with fenbendazole again...
Seven abyssinian cats (two male, five female) showed intermittent green-yellow mucous diarrhoea, sometimes an inflammation of the anal region and faecal incontinence even after long-time treatment with fenbendazole against Giardia. During necropsy of one of the cats, which had to be euthanized due to another disease, the gut wall of small and large intestine appeared macroscopically thickened. Histological examination indicated flagellates in the lumen of the intestine (initiating at the jejunum) and in the crypts. However Giardia could be excluded. in this case. By PCR of the faeces Tritrichomonas (T) foetus was diagnosed in five of six cats of this colony. Five remaining animals (another cat had to be euthanized) were treated with about 30 mg per kg BW ronidazole p. o. (rededication; Ridzol 10% Bt®, Dr. Hesse Tierpharma GmbH & Co. KG, Germany) daily over 14 days. The special gastro-resistant processing of the ronidazole should ensure a targeted effects. Animals were treated consecutively, isolated from the other cats and were daily examined clinically and neurologically. Neurotoxic adverse effects appeared slightly, therefore--as a precaution--the treatment of two cats was paused for one day. After treatment of all cats, T. foetus wasn't diagnosed by PCR over the period of 345 to > 800 days in any cat. One animal had dubious findings in the ninth week after treatment. Hence it was still kept isolated from the group and PCR showed a negative result at all times afterwards. The treatment protocol shows that elimination of problematic protozoal infections is possible in cat colonies.
Comparison of selected haematological and biochemical indices and behaviour patterns of pheasant hens kept in different housing systems during the laying period.
Voslarova E, Bedanova I, Radisavljevic K … +3 more , Hrabcakova P, Marsalek P, Vecerek V
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26591380
Selected haematological and biochemical indices and behaviour patterns were monitored in cage-housed pheasant hens during the peak-of-lay egg laying period. Lower counts of monocytes (p = 0.035) and concentrations of pla...
Selected haematological and biochemical indices and behaviour patterns were monitored in cage-housed pheasant hens during the peak-of-lay egg laying period. Lower counts of monocytes (p = 0.035) and concentrations of plasma biopterin (p = 0.020) and higher concentrations of plasma neopterin (p = 0.005) and plasma phosphorus (p = 0.025) were found in spectacles-fitted pheasant hens kept in conventional cages compared to non-spectacled hens kept in enriched cages. Even more pronounced effects of the housing system were revealed by analysis of the behaviour of pheasant hens: spectacles-fitted pheasant hens kept in conventional cages exhibited higher occurrences of movement (p = 0.045), stereotyped behaviour (p = 0.039), and aggression (p = 0.004), and lower rates of feeding (p = 0.009), drinking (p < 0.001), defecation (p = 0.038), preening (p = 0.013), and feather pecking (p < 0.001). Our results show that the relatively easy and inexpensive enrichment of the cage environment (two perches, a simple hideout) where breeding groups of common pheasants are housed during the laying period can significantly contribute to the health and welfare (including the ability to express natural behaviour patterns) of pheasants kept in commercial cage systems.
Multicentric T-cell lymphoma accompa- nied by infiltration of the peripheral and central nervous system in a fattening pig.
von Altrock A, Ganter M, Schwittlick U … +1 more , Beineke A
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26591379
Multicentric lymphomas, usually of B-cell origin, are rather common neoplasms in pigs, which usually affect lymph nodes and different organ systems. An uncommon manifestation of malignant lymphoma represents neoplastic p...
Multicentric lymphomas, usually of B-cell origin, are rather common neoplasms in pigs, which usually affect lymph nodes and different organ systems. An uncommon manifestation of malignant lymphoma represents neoplastic peripheral nerve involvement, which has been reported in human medicine. So far, only single cases of lymphoma with manifestation in peripheral nerves (known as neurolymphomatosis) without leukemic conversion have been mentioned in different animal species others than swine. The present case report describes the occurrence of a multicentric T-cell lymphoma in a fattening pig with marked leukocytosis. The pig was presented with symptoms of lethargy, weight loss, and fever. Bone marrow aspirate contained all stages of granulocytic and erythrocytic precursors, and a remarkable large number of eosinophiles and undifferentiated blasts. At necropsy, the carcass showed generalized lymphadenopathy and severe hepatosplenomegaly. Histological findings included neoplastic round cell infiltrations in lymph nodes, bone marrow, tonsils, lung, spleen, liver, kidneys, urinary bladder, small and large intestine, and meninges. Additionally, neoplastic cells were present in the sciatic and medial plantar nerve of the left hind leg. The diagnosis T-cell lymphoma was confirmed immunohistochemically. To the author's knowledge, this work represents the first report of peripheral nerve involvement in a pig with multicentric T-cell lymphoma accompanied by CNS involvement and leukemia.
Impact of diet on 24-hour intragastric pH profile in healthy horses.
Damkel C, Snyder A, Uhlig A … +2 more , Coenen M, Schusser GF
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26591378
An electrode incorporated into a polyethylene hose was introduced under endoscopic control into the stomach of six fasting adult horses for long-lasting pH measurements. The intragastric pH was recorded every four second...
An electrode incorporated into a polyethylene hose was introduced under endoscopic control into the stomach of six fasting adult horses for long-lasting pH measurements. The intragastric pH was recorded every four seconds for a period of 24 hours. The Warmblood horses were assigned randomly to receive hay ad libitum (H group); 1.5 kg hay/100 kg BW/day and 1 kg concentrate/100 kg BW/ day (C group) or protocol C plus 75 g pectin-lecithin supplement/100 kg BW/day (P group). The horses were adapted to each diet for 14 days. The 24-hour median pH value for protocol H (2.69) was significantly lower compared to protocol C (3.35) and P (3.44) (p < 0.05). The horses in protocol P had a significant higher percentage (40.1 %) of 24-hour intragastric pH values ≥ 4 than in protocol C (36.2 %) or in protocol H (25.3 %) (p < 0.05).
Mercury concentrations in feathers of Common Swifts (Apus apus).
Legler M, Leonhard W, Koch NJ … +1 more , Kummerfeld N
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281449
Mercury (Hg) has been well studied as a bioaccumulated contaminant in aquatic ecosystems worldwide. It has been found to have negative effects on carnivorous and piscivorous bird species with the highest Hg concentration...
Mercury (Hg) has been well studied as a bioaccumulated contaminant in aquatic ecosystems worldwide. It has been found to have negative effects on carnivorous and piscivorous bird species with the highest Hg concentrations at the top of the food chain. The objective of this study was to increase our knowledge of mercury exposure in insectivorous birds, especially in a species of the family Apodidae. The Common Swift (Apus apus) that specialises on feeding on aerial plankton molts and winters as a long-distance migrant in Sub-Saharan Africa. In the breeding seasons 2011-2013 the concentrations of Hg in primary flight feathers (P8-P10) in juvenile (n = 35) and adult (n = 25) injured Common Swifts were examined in the area of Hannover, Lower Saxony, Germany using cold vapour atomic absorption spectrometry. As a first reference, it was possible to determine feather Hg levels in adult (Mean ± SD: 0.53 mg/kg wet weight ± 0.37; Xmin-Xmax: 0.04-1.45 mg/kg; n = 25) as well as in juvenile Swifts (Mean ± SD: 0.11 mg/kg wet weight ± 0.06; Xmin-Xmax: 0.04-0.25 mg/kg; n = 35). The significant differences between the ages (p ≤ 0.001) are probably caused by differences in the feather growth, in accumulation of mercury for a longer time in adults as well as particularly in differences between mercury pollution in breeding and wintering ground. The mercury levels detected in swifts in this study are all below the range found by other authors to cause behavioural changes or reduced reproduction (5 mg/kg).
Elevated testosterone levels in a racing horse due to an XY testicular disorder of sexual development.
Dierks C, Sieme H, Piechotta M … +5 more , Lehner S, Merkt JC, Uphaus H, Klug E, Distl O
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281448
A female thoroughbred successful in horse racing was positively tested for high testosterone values. This horse neither showed stallion-like-behaviour nor signs of ambiguous external genitalia. The karyotype of this hors...
A female thoroughbred successful in horse racing was positively tested for high testosterone values. This horse neither showed stallion-like-behaviour nor signs of ambiguous external genitalia. The karyotype of this horse was 2n = 64,XY and the sex-determining region of Y (SRY) PCR was positive. Hair samples tested for naturally testosterone revealed values normal for stallions, and tests for eight synthetic testosterone esters remained negative. The phenotype, ultrasonographic examination, hormone status, cytogenetic evaluation and molecular diagnostics lead to the diagnosis of an XY testicular disorder of sexual development (DSD) due to a complete androgen insensitivity syndrome. To our knowledge this is the first report about a thoroughbred in racing sports with an XY testicular disorder of sexual development. To date, intersex racing horses have never been described in thoroughbreds or a regulation for intersexes in regard to horse races has been issued.
Effectiveness of salicylic acid paste for treatment of digital dermatitis in dairy cows compared with tetracycline spray and hydrotherapy.
Kofler J, Innerebner C, Pesenhofer R … +2 more , Hangl A, Tichy A
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281447
The effectiveness of salicylic acid paste (PASTE) was tested for topical treatment of 25 acute and 25 chronic digital dermatitis (DD) lesions. Control groups with the same number of acute and chronic DD lesions were trea...
The effectiveness of salicylic acid paste (PASTE) was tested for topical treatment of 25 acute and 25 chronic digital dermatitis (DD) lesions. Control groups with the same number of acute and chronic DD lesions were treated with topical oxytetracycline spray (SPRAY) and by washing only with water (HYDRO) respectively. The therapeutic effects were evaluated using a pain score, the healing rate, the lesion size and other parameters. Pre-treatment and control examinations were carried out on day 0, 4, 14 and 21. In the PASTE group, 76.0% of acute DD lesions were pain free and 64.0% of acute DD lesions were healed on day 21 showing a normal skin surface (MO). Only 28.0% of acute DD lesions treated with SPRAY and 16.0% treated with HYDRO had healed on day 21. A significantly higher healing rate was revealed in acute lesions for the PASTE compared to the HYDRO group (p < 0.05) for all three re-checks, and for the PASTE group compared with the SPRAY group (p < 0.05) for day 4 and day 14. Healing rates of chronic DD lesions were higher in the PASTE group with 44.0% on day 14 and 36.0% on day 21, compared with 16.0% in the SPRAY and 32.0% in the HYDRO group on day 14, and 20.0% (SPRAY) and 28.0% (HYDRO) on day 21 respectively. The recurrence rate of lesions after they had healed during the study period was 14.5% in total. Digital dermatitis lesions treated with salicylic acid paste and a wrap showed significantly higher healing rates within the study period, odds ratios for healing of acute lesions with PASTE were 4.5 to 6.7 times higher than with SPRAY, and 9.3 to 36.4 higher compared with HYDRO.
[Relationship and repeatability of body condition scoring and backfat thickness measurement in dairy cows by different investigators].
Pothmann H, Erlen A, Pichler M … +2 more , Huber J, Drillich M
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281446
The determination of the body condition of dairy cows is a helpful instrument to assess the energy situation of individual cows and herds. Two methods for determining the body condition are well established in bovine pra...
The determination of the body condition of dairy cows is a helpful instrument to assess the energy situation of individual cows and herds. Two methods for determining the body condition are well established in bovine practice, body condition scoring (BCS) and measurement of backfat thickness (BFT). The objectives of this study were to evaluate the repeatability of BCS on a five-point scale with 0.25-points increments and the measurement of BFT by ultrasound (5 MHz linear probe) as well as the repeatability of measuring BFT at both sides of the cows back. Five investigators with different experience with BCS and BFT assessed a total of 94 cows repeatedly, resulting in 1806 BCS-measurements and 1723 (left) and 1733 (right) BFT-values. Weighted kappa coefficient was used to evaluate the agreement of repeated measurements and correlations were calculated for linear associations. Within-observer agreement of BCS and BFT was good for both methods (K = 0.67 and 0.78, respectively). Agreement was moderate to substantial for BCS and BFT depending on the investigator. Within-observer agreement of BFT at the right and left body side was substantial (K = 0.75). There was a high correlation between repeated measurements of BCS and BFT (r = 0.93 and r(c) = 0.91, respectively), and between BFT measured at the left and right body side (r(c) = 0.90). The correlation between BCS and BFT was moderate (r = 0.67). Overall, both methods demonstrated good repeatability applied by different investigators. In summary, BCS and BFT measurements are practical tools to contribute beneficially to herd health management.
Ectoparasites of hedgehogs (Erinaceus concolor) from Turkey.
Girisgin AO, Senlik B, Aydin L … +1 more , Cirak VY
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281445
Hedgehogs are small, nocturnal, spiny-coated animals that have been growing in popularity as exotic pets. However, these animals are host to a wide variety of viruses, bacteria, fungi and parasites, some of which are of...
Hedgehogs are small, nocturnal, spiny-coated animals that have been growing in popularity as exotic pets. However, these animals are host to a wide variety of viruses, bacteria, fungi and parasites, some of which are of zoonotic character. Thus, because hedgehogs have a potential role to transmit zoonoses including arthropod-borne diseases, we examined them for their ectoparasites. The study was carried out on hedgehogs found dead mainly due to road casualties in the Bursa province of Turkey. The ectoparasites were collected by both insecticide spraying of the body and inspection on a white paper carefully. Totally three species of ticks (Rhipicephalus sanguineus, Hyalomma aegyptium, Haemophysalis parvo) and one flea species (Archeopsylla erinacei) were detected. The prevalence of mixed infestation with both ticks and fleas was 45.5%. Haemaphysalis parva was reported for the first time from hedgehogs (Erinaceus concolor) in Turkey. The occurrence of ectoparasites and their potential role as vectors of certain zoonotic diseases are briefly discussed.
The occurrence of Anaplasma phagocyto- philum in wild bison from the Bialowieza Primeval Forest in Eastern Poland.
Dzięgiel B, Adaszekl Ł, Krzysiak M … +4 more , Skrzypczak M, Adaszek M, Furmaga B, Winiarczyk S
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281444
The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anapla...
The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 183908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum.
Comparison of the prevalence of Mycoplasma species in dogs with and without respiratory disease.
Schulz BS, Raufeisen K, Weber K … +2 more , Laberke S, Hartmann K
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281443
Aim of the study was to investigate the prevalence of Mycoplasma species in dogs with and without signs of respiratory disease. Bronchoalveolarlavage fluid (BALF) and pharyngeal swabs were collected from 29 dogs with res...
Aim of the study was to investigate the prevalence of Mycoplasma species in dogs with and without signs of respiratory disease. Bronchoalveolarlavage fluid (BALF) and pharyngeal swabs were collected from 29 dogs with respiratory diseases (RD) and 16 dogs without signs of RD that were euthanised because of other diseases. Samples were tested for Mycoplasma species by PCR and culture, and sequencing was performed in Mycoplasma species-positive BALF samples. Pharyngeal swabs were positive for Mycoplasma species by PCR in 91.7% of dogs with RD and 86.7% of dogs without signs of RD (p = 1.000); BALF samples were PCR-positive in 37.9% of dogs with RD and 18.8% of dogs without signs of RD (p = 0.194) Mycoplasmo culture of BALF was positive in 28.6% of dogs with RD and in 18.8% without signs of RD (p = 0.730). When culture and PCR were compared, there was no significant difference in the detection rate of Mycoplasma species (p = 0.658) Sequencing detected different Mycoplasma species. Out of these, however, Mycoplasma cynos was isolated from four dogs with RD. There is no significant difference in the prevalence of Mycoplasma species between dogs with RD and dogs without evidence of RD; however, Mycoplasma cynos seems to be associated with respiratory disease.
[Prevalence of antibodies against Tick-Borne Encephalitis virus in dogs from Saxony, Germany].
Balling A, Beer M, Gniel D … +1 more , Pfeffer M
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281442
Tick-borne encephalitis (TBE) is the most important tick-transmitted viral disease in Europe and is caused by the TBE virus (TBEV), a member of the Flaviviridae family. In Germany, the vast majority of human TBE cases oc...
Tick-borne encephalitis (TBE) is the most important tick-transmitted viral disease in Europe and is caused by the TBE virus (TBEV), a member of the Flaviviridae family. In Germany, the vast majority of human TBE cases occurs in the south in so-called risk areas. However, in areas with only sporadic TBE cases, the respective risk assessment is hard to achieve. We therefore intend to use the prevalence of antibodies against TBEV in dogs as an indicator to trace such TBE endemic areas. Between August 2012 and March 2014, a total of 331 blood sera were collected from dogs all over Saxony, which hadn't left the state for the past five years. For the detection of antibodies against TBE-virus a commercial ELISA was used. Ten sera with positive or borderline ELISA results were retested by serum neutralization test. All seven ELISA-positive serum samples could be verified to contain TBE-virus-specific antibodieswith SNT titres between 1:15 and more than 1:40. We therefore found 2.1% seroprevalence in our samples. We conclude, that dogs can very well be used as sentinels, especially in areas with only sporadic TBE cases, although larger sample sizes are desired.
Platelet function in dogs with bacterial infections and leishmaniasis.
Abid M, Kalbantner K, Mischke R
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281441
The objective of this study was to examine the influence of bacterial infections or leishmaniasis on primary haemostasis in dogs. Capillary bleeding time, automatic platelet function analysis (PFA-100), turbidimetric pla...
The objective of this study was to examine the influence of bacterial infections or leishmaniasis on primary haemostasis in dogs. Capillary bleeding time, automatic platelet function analysis (PFA-100), turbidimetric platelet aggregation, impedance aggregometry, platelet count and, in addition, the haematocrit were investigated in 25 dogs with bacterial infections or leishmaniasis . Results of these diseased dogs were compared to the control group and additionally classified into two subgroups based on criteria of systemic inflammatory response syndrome (SIRS) (groups "SIRS" and "Non-SIRS"). Dogs with infections had a significantly prolonged closure time of the PFA-100 using both cartridges (e. g., collagen/ADP: 83 [55-301] vs. 65 [47-99 s; median [minimum-maximum]; p < 0.0001), a significant decrease in maximal aggregation of the turbidimetric aggregometry (e. g., ADP-induced: 45.2 ± 26.8 vs. 67.3 ± 21.8%; mean ± SD; P = 0.003), a significant increase of collagen-induced impedance aggregometry and a significant suppression of arachidonic acid-induced impedance aggregometry. An enhanced collagen-induced impedance aggregation was the only significant difference between subgroups "SIRS"and "Non-SIRS". In conclusion, although individual tests indicate enhanced platelet aggregation, most of the in vitro tests revealed a normal to moderately reduced functionality. The reduced aggregabiity may partly indicate preactivation of platelets.
[Severe purulent and necrotizing glossitis in a fallow deer (Dama dama) due to an infection with the involvement of Mannheimia granulomatis].
Kupca AM, Rettinger A, Zimmermann P … +3 more , Hörmansdorfer S, Konrad R, Hafner-Marx A
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281440
Post mortem examination of a young fallow deer (Dama dama) revealed a severe purulent and necrotizing glossitis as well as a multifocal necrotizing and ulcerative rumenitis and typhlitis. The animal was cachectic. Mannhe...
Post mortem examination of a young fallow deer (Dama dama) revealed a severe purulent and necrotizing glossitis as well as a multifocal necrotizing and ulcerative rumenitis and typhlitis. The animal was cachectic. Mannheimia (M.) sp. was isolated from the tongue lesions and identified as M. granulomatis by MALDI-TOF MS and 16S rRNA sequencing. Mycosis and BVDV infection were excluded. Few publications are dealing with similar macroscopic findings associated with the isolation of M. granulomatis in cattle and roe deer. Therefore, M. granulomatis should also be taken into consideration when such lesions occur in other ruminants. Based on our findings in case of gross pathological lesions of the tongue of ruminants a Mannheimia granulomatis-infection should be investigated as well as the possible role of Fusobacterium necrophorum, Actinobacillus lignieresii or Actinomyces bovis.
Spontaneous bacterial and fungal infections in genetically engineered mice: Is Escherichia coli an emerging pathogen in laboratory mouse?
Benga L, Benten WP, Engelhardt E … +2 more , Gougoula C, Sager M
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281439
The impact of particular microbes on genetically engineered mice depends on the genotype and the environment. Infections resulting in clinical disease have an obvious impact on animal welfare and experimentation. In this...
The impact of particular microbes on genetically engineered mice depends on the genotype and the environment. Infections resulting in clinical disease have an obvious impact on animal welfare and experimentation. In this study, we investigated the bacterial and fungal aetiology of spontaneous clinical disease of infectious origin among the genetically engineered mice from our institution in relation to their genotype. A total of 63 mice belonging to 33 different mice strains, from severe immunodeficient to wild-type, were found to display infections as the primary cause leading to their euthanasia. The necropsies revealed abscesses localized subcutaneously as well as in the kidney, preputial glands, seminal vesicles, in the uterus, umbilicus or in the lung. In addition, pneumonia, endometritis and septicaemia cases were recorded. Escherichia coli was involved in 21 of 44 (47.72%) of the lesions of bacterial origin, whereas [Pasteurella] pneumotropica was isolated from 19 of 44 (43.18%) cases. The infections with the two agents mentioned above included three cases of mixed infection with both pathogens. Staphylococcus aureus was considered responsible for five of 44 (11.36%) cases whereas Enterobacter cloacae was found to cause lesions in two of 44 (4.54%) mice. Overall, 16 of the 44 (36.36%) cases of bacterial aetiology affected genetically engineered mice without any explicit immunodeficiency or wild-type strains. The remaining 19 cases of interstitial pneumonia were caused by Pneumocystis murina. In conclusion, the susceptibility of genetically modified mice to opportunistic infections has to be regarded with precaution, regardless of the type of genetic modification performed. Beside the classical opportunists, such as [Pasteurella] pneumotropica and Staphylococcus aureus, Escherichia coli should as well be closely monitored to evaluate whether it represents an emerging pathogen in the laboratory mouse.
[Detection of Coxiella burnetii in dairy cattle bulk tank milk and single tank milk samples by confirmatory testing].
Hilbert A, Andres T, Werner R … +4 more , Wehr R, Fröhlich A, Conraths FJ, Henning K
Berl Munch Tierarztl Wochenschr · 2015 · PMID 26281438
Q fever is a worldwide zoonotic disease caused by the pathogen Coxiella (C.) burnetii. A wide range of animal species is susceptible to this intracellular bacterium with great importance in ruminants. Human infections oc...
Q fever is a worldwide zoonotic disease caused by the pathogen Coxiella (C.) burnetii. A wide range of animal species is susceptible to this intracellular bacterium with great importance in ruminants. Human infections occur mainly by airborne transmission. C burnetii was detected in animal products such as raw milk, raw-milk cheese and butter prepared from raw milk as well as in the meat of infected animals. In cattle milk, the pathogen was detected up to 13 months after calving. The risk of human foodborne C. Burnetii infection is still considered to be low, but cannot be completely ruled out and remains under discussion. The aim of this study was to compare different laboratory diagnostic methods for C. burnetii in milk sample. The bulk tank and individual milk samples were sent and studied at the National Reference Laboratory for Q-fever in the context of confirmatory laboratory testing after clinical suspicion or retesting of previously antibody detection was in the analysis of 888 individual milk samples a match of 93.3% (Cohen-kappa). A total of 173 bulk milk samples and 2,807 individual milk samples from bovine herds for the presence of C. burnetii DNA and antibodies were tested against the pathogen. The pathogen was detected in 62.5% of the bulk milk samples and up to 60% in individual milk samples. The highest proportion of positive bulk milks was determined as 68.3% in 2012. In individual milk samples, the highest proportion of seropositive samples was 62.2%.
About
- Frequency
- Sun
- Papers found
- 200
- RSS feed
- Subscribe