Zhonghua Er Bi Yan Hou Ke Za Zhi[JOURNAL]
Sun
200 papers
[Immunohistochemical diagnosis of fungus ball in paranasal sinuses].
Li Y, Yang DZ, Pan L … +2 more , Liu DD, Zhang XB
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15606005
OBJECTIVE: To study the pathogens of fungus balls in paranasal sinuses and establish an immunohistochemical test by which the main opportunistic fungi could be identified. METHODS: Twenty-five patients with fungal balls...
OBJECTIVE: To study the pathogens of fungus balls in paranasal sinuses and establish an immunohistochemical test by which the main opportunistic fungi could be identified. METHODS: Twenty-five patients with fungal balls were treated by surgical removal of the fungus ball in the infected sinuses. The pathogenic fungi on the specimens were identified by means of routine PAS and immunohistochemical staining methods, and the sensitivity of the two methods were compared. RESULTS: The most commonly infected sinus was the maxillary sinus, followed by sphenoid sinus. Pathogens of fungal balls were found to be aspergillus (92%, 23/25) and candida 2 cases (8%). Aspergillus and candida albicans in infected sinuses could be specifically identified by immunostainning. There was no statistically significant differences of sensitivity between immunostainning and PAS stain. CONCLUSIONS: The main pathogen of the fungus ball was aspergillus. Immunostainning was a rapid and reliable method to identify fungi in infected tissues of paranasal sinuses. It should be widely used in the diagnosis of fungal sinusitis.
[Diagnosis and therapy of glomus tympanicum and glomus jugulare tumors].
Liu JF, Ni DF, Gao ZQ … +3 more , Xu CX, Li WY, Chen XM
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15606004
OBJECTIVE: To report on a series of patients with glomus tympanicum or glomus jugulare tumors, and to focus on its diagnosis, treatment, and outcomes. METHODS: Ten patients with glomus tympanicum or glomus jugulare tumor...
OBJECTIVE: To report on a series of patients with glomus tympanicum or glomus jugulare tumors, and to focus on its diagnosis, treatment, and outcomes. METHODS: Ten patients with glomus tympanicum or glomus jugulare tumors at Peking Union College Hospital during a 17-year period were reviewed retrospectively. RESULTS: There were 7 patients with glomus tympanicum, 3 with glomus jugulare tumors, 1 patient with familial paraganglioma and 1 with functioning glomus jugulare tumors. The most common presenting symptoms were pulsatile tinnitus and hearing loss. Other symptoms included facial nerve paralysis, vertigo, otalgia, dysphagia, hoarseness, throat sore, episodic hypertension with headaches and tachycardia. The most common physical sign was a vascular middle ear mass. The other physical signs included Brown sign, upper neck mass, pharyngeal plump, Collet-Sicard syndrome, Homer's syndrome as well as deficit of cranial nerve V. The radiographic evaluation included computed tomograph (9 cases), angiography (4 cases) and magnetic resonance imaging (1 case). Eight patients initially refered to ENT department, and 2 patients initially consulted neurology or endocrinology specialists. The treatment included preoperative embolization in 2 cases, simple surgery in 4 cases and surgery followed by radiation therapy in 6 cases. No significant complications occurred. Nine of the 10 patients were followed up, but 1 was lost. The mean follow-up time was 12 years (ranged, 2-19 years). No tumor recurrence occured in the 6 cases with total tumor removal. Three cases with subtotal tumor resection had no tumor progression. CONCLUSIONS: The diagnosis and treatment of glomus tympanicum and glomus jugulare tumors is particularly challenging. Typical clinical manifestations and radiographic evaluation should be considered together to establish the diagnosis. The primary treatment for glomus tympanicum is surgery, if necessary, followed by radiotherapy. Subtotal tumor resection followed by radiation yields satisfying outcome for glomus jugulare tumors.
[Mutation screening in selected exons of myosin 7a gene in prelingual non-syndromic hearing impairment patients].
Hu P, Xie DH, Xiao ZA … +4 more , Wu WJ, Ge SL, Hu ZM, Xia K
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15606003
OBJECTIVE: To ascertain the frequency and characteristics of myosin 7a gene mutations in Chinese with prelingual nonsyndromic hearing impairment. METHODS: Most of cases were collected within Hunan province, including 31...
OBJECTIVE: To ascertain the frequency and characteristics of myosin 7a gene mutations in Chinese with prelingual nonsyndromic hearing impairment. METHODS: Most of cases were collected within Hunan province, including 31 sporadic congenital deaf patients and 65 patients from 34 hereditary prelingual deafness families, and 100 health individuals were used as control. Genomic DNA was extracted from the patients and subjected to the PCR to amplify selected exons of myosin 7a gene, and then the amplified products were screened for base variations by single strand conformational polymorphismanalysis (SSCP). The bands with abnormal conformation were sequenced to confirm the mutation. RESULTS: G to A substitution was detected at nucleotide 617 in exon 7 as hetrozygous state in two patients and was not found in unaffected members in their family. This mutation caused Arg206Gln within a highly conserved heptapeptide sequence of myosin 7a protein, and was close relevant to the prelingual nonsyndromic deafness. CONCLUSIONS: The Arg206Gln mutation in exon 7 of myosin 7a is possibly a novel mutation to cause prelingual nonsyndromic hearing impairment. Our results provide the evidence that exon 7 of Myosin 7a is a mutational hotspot region in genetic deafness.
[Study on the phenomenon of regionally pathological difference of otitis media and its clinical significance].
Zhang QA, Zhang XT, Wu CQ … +1 more , Zhang Q
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15606002
OBJECTIVE: To study the feature and difference of severity of pathological changes in different areas of the middle ear cleft with otitis media (OM) and its pathogenesis and clinical significance. METHODS: The temporal b...
OBJECTIVE: To study the feature and difference of severity of pathological changes in different areas of the middle ear cleft with otitis media (OM) and its pathogenesis and clinical significance. METHODS: The temporal bone slides of 290 ears with OM with inflammatory effusion had been studied histopathologically under microscope. The histopathological change in different areas has been comparatively observed. 256 cases with various forms of chronic otitis media (COM) have been studied with high-resolution CT, and 189 cases with COM were observed during operation, stressing on the difference of pathogical changes in different areas. RESULTS: In the process of OM, the mucosa of the eustachian tube almost has no inflammatory pathological changes, and in hemi-anterior and infer-posterior mesotympanum area was much lightly and reversible pathological change. But the pathological changes in the ossicular chain area was much more serious than the other two areas, and was irreversible or intractable such as granulation tissue, cholesteatoma etc. CONCLUSIONS: Because of the historical and anatomical distinction difference between anterior and posterior area of the middle ear cleft and blockage of internal ventilation/draining system in process of OM, the stagnancy of inflammatory effusion and formation of granulation tissue in posterior area are the main cause leading to the phenomenon of regionally pathological difference in different areas of the middle ear clefts.
[Expression of prolactin in macrophage of nasal polyps and its significance].
Guan B, Dong Z, Yang ZQ … +1 more , Guan GM
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15606001
OBJECTIVE: To detect the expression level and distribution of prolactin (PRL) in nasal polyp and to find out the significance of the mechanism of PRL in the invasion of nasal polyp. METHODS: Twenty-five polyp tissues wer...
OBJECTIVE: To detect the expression level and distribution of prolactin (PRL) in nasal polyp and to find out the significance of the mechanism of PRL in the invasion of nasal polyp. METHODS: Twenty-five polyp tissues were obtained from the patients who were subjected to nasal polypectomy in our Department. Inferior turbinate mucosa was used as control obtained from 12 patients with rhinogenous snoring. HE staining was performed for routine histopathologic examination. The expression of PRL in nasal polyps was observed by immunohistochemical staining, and six polyp tissues were estimated through double staining for determining cells which expressed PRL. RESULTS: (1) Positive expression of PRL was significantly stronger (t =4.004, P < 0.01) in 25 nasal polyp tissues (2.05 +/- 0.88) than that in 12 normal inferior turbinate mucosa (0.96 +/- 0.50). Positive expression of macrophage (CD68) was significantly stronger (t = 3.519, P < 0.01) in 25 nasal polyp tissues (1.85 +/- 0.83) than that in 12 normal inferior turbinate mucosa (0.93 +/- 0.52). (2) The PRL expressing cell mainly was the macrophage as demonstrated by double immunohistochemical method. CONCLUSION: PRL derived from macrophages of nasal mucosa may participate in the formation of nasal polyp through its local immune modulation.
[Anatomical landmarks of skull base and safety of the skull base surgery].
Liu YH, Zhang KL, Yang KL … +6 more , Wan JH, Yang JM, Lu DH, Qiu JX, Fang P, Tong BS
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15606000
OBJECTIVE: In order to improve the safety of skull base surgery and to completely resect the skull base tumors, the anatomical landmarks of skull base were studied. METHODS: 29 cases of skull base surgery were performed...
OBJECTIVE: In order to improve the safety of skull base surgery and to completely resect the skull base tumors, the anatomical landmarks of skull base were studied. METHODS: 29 cases of skull base surgery were performed between 1992 and 2002, and their clinical data were retrospectively analysed. The anatomical landmarks of cranial base, such as comb, pterygoid process, spine of temporal bone, styloid process, and internal caroid artery, were analysed in preoperative diagnosis and operative treatment of various kinds of skull base tumors. RESULTS: In the early stages, because of lacking the knowledge of anatomical landmarks of the skull base, the surgery lasted longer, part of skull base tumors remained and operative blood lose was much more than that in later stage. In the later stage, no operative death and severe complications were found in 26 cases which underwent various kinds of skull base surgery. The survival rates at 3 and 5 years were 72.2% and 35.7% respectively for malignant tumors. CONCLUSIONS: Comb, pterygoid process, spine of temporal bone, styloid process, internal caroid artery and their adjacent structures were important anatomical landmarks for operative treatment of anterior, middle and lateral cranial base tumors. It was important to know the anatomical landmarks to ensure the safety of the skull base surgery.
[Surgical treatment of transcranial middle cranial fossa-lateral skull base malignant tumor].
Yuan YW, Zhen ZN, Zhao M … +4 more , Chen H, Chen XM, Li JZ, Yang PW
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15605999
OBJECTIVE: To evaluate the surgical method of transcranial middle cranial fossa-lateral skull base malignant tumor. METHODS: Sixteen patients with transcranial middle cranial fossa-lateral skull base malignant tumors wer...
OBJECTIVE: To evaluate the surgical method of transcranial middle cranial fossa-lateral skull base malignant tumor. METHODS: Sixteen patients with transcranial middle cranial fossa-lateral skull base malignant tumors were treated by three surgical methods. (1) Postauricular "C" form incision, combined with subtotal resection of temporal bone and high lateral neck approach to resect tumors located in middle cranial fossa, lateral skull base, and parotid region. (2) Temporal side "tungue" form incision, middle cranial fossa approach to resect tumors inside and outside the skull. (3) Temporal-auricular-mandible swing approach to resect tumors in parapharyngeal-infratemporal fossa-middle cranial fossa. Thirteen of the 16 cases received radiotherapy postoperatively. RESULTS: After operation, 11 of the 16 cases survived more than three years, five cases died within three years. Three-year-survival rate was 69%. CONCLUSIONS: Our results showed that the three surgical methods adopted could fully expose the internal and external skull lesion to remove the extensive malignant tumors. For late stage malignant tumors of the skull base, it should be treated aggressively by combination of surgical therapy and postoperative radiotherapy.
[Endoscopic or endoscope-assisted surgery for sinonasal malignancy].
Wang RG, Lei L, Han DY … +1 more , Liu LF
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15605998
OBJECTIVE: To discuss the feasibility and therapeutic efficacy of resection of sinonasal malignant tumours through endoscopic or endoscope-assisted surgery. METHODS: Since 1997, 11 cases of malignant tumours were resecte...
OBJECTIVE: To discuss the feasibility and therapeutic efficacy of resection of sinonasal malignant tumours through endoscopic or endoscope-assisted surgery. METHODS: Since 1997, 11 cases of malignant tumours were resected under endoscope or by endoscope-assisted surgery. Among them, 5 patients were followed-up for more than 3 years, including one case of nasal septum squamous cell carcinoma; one case of ethmoid sinus squamous cell carcinoma; one case of maxillary sinus adenoid cystic carcinoma and two cases of olfactory neuroblastoma. RESULTS: In the patients who were followed-up for more than three years, no local recurrence was found. There was only one case of cervical lymph node metastasis found on a patient with olfactory neuroblastoma 2. 5 years after resection. Four years after operation, this patient was suspected to have extensive metastasis in meninges, and only palliative treatment was suggested. CONCLUSIONS: By endoscope or by endoscope-assisted surgery, some of selected sinonasal malignant tumours can be thoroughly resected. The advantages are mini-invasive and avoidance of facial scar so as to improve the quality of life (QOL) of patients. Large amount of cases, random clinical study with lone- term follow-up are still needed to verify the feasibility and efficacy of endoscopic or endoscope-assisted surgery.
[Clinical analysis of 430 cases of skull base lesions].
Huang DL, Yang WY, Han DY … +3 more , Wang RG, Wang JL, Zhou QY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15605997
OBJECTIVE: Through analysis of surgical treatment and repair methods for the skull base lesions, to summarize the type, sites, characters of the lesions and the methods of surgical treatment and repair, and to provide th...
OBJECTIVE: Through analysis of surgical treatment and repair methods for the skull base lesions, to summarize the type, sites, characters of the lesions and the methods of surgical treatment and repair, and to provide the beneficial experience for the surgeons. METHODS: Four hundred and thirty patients were hospitalized and treated surgically between Feb. 1982 and Dec, 2002, and their clinical data were analyzed. RESULTS: Of 430 patients, 265 (61.6%) were male, 165 (38.4%) female. The mean age was 36. 1 years old (range from 2.5 to 76.0). In 173 cases (40.2%), the lesions were on the left side of the skull base, 178 cases (41.4%) on the right, 79 cases (18.4%) involving the central and both side of the skull base. In 116 cases (27.0%), the lesions were located in anterior skull base, 57 (13.3%) in central, 174 (40.5%) in lateral and posterior, and the remainder 83 cases (19.5%) in two or more regions. Of 430 cases, 283 (65.8%) were tumors, among them, 154 (35.8%) were benign, 129 (30.0%) malignant, 76 (17.7%) trauma, 42 (9.8%) congenital, 18 (4.2%) infection, 11 (2.6%) unknown. All patients were surgically treated. Of them, 43 cases were treated for 2 times, 5 cases for 3 times, 4 for 4 times, 1 for 5 times, 3 for 6 times. The total were 514 times. The bone defect was reconstructed in 95 cases (22.1%). The dura mater was repaired in 117 cases (27.2%). The postoperative complications occurred in 27 cases. Two cases died in 2 and 3 days after operation because of brain hernia with the ligation of common carotid artery and internal carotid artery for artery rupture. No death occurred during operation. CONCLUSIONS: The majority of the skull base lesions were tumors. The male patients were more than female. Appropriate surgical approach and better perioperative treatment could harvest the satisfactory effect.
[Skull base: its significance to the otorhinolaryngologists].
Yang WY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Sep · PMID 15605996
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[Gene expression of matrix metalloproteinase 14 in laryngeal squamous cell carcinoma].
Liu YH, Tang PZ, Liu ZH … +6 more , Xu ZG, Qi YF, Li AD, Ding F, Zhang LY, Luo AP
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563089
OBJECTIVE: To investigate the role of expression of matrix metallo-proteinase 14 (MMP14) in laryngeal carcinomas and the relationship between MMP14 expression and the laryngeal biological behavior. METHODS: The gene expr...
OBJECTIVE: To investigate the role of expression of matrix metallo-proteinase 14 (MMP14) in laryngeal carcinomas and the relationship between MMP14 expression and the laryngeal biological behavior. METHODS: The gene expression differences of MMP14 between fresh laryngeal cancer tissues and their surrounding normal mucosa was analyzed by RT-PCR. The expression of MMP14 protein in paraffin-embedded tissues was determined immunohistochemically. All statistical analyses were performed by SPSS version 10.0. RESULTS: In the 33 cases of matched specimens, MMP14 gene expression was much higher in tumor tissues than that in surrounding ing normal tissues in 26 cases and lower in 2 cases, whereas in other 5 cases, no significant difference was observed between the cancer tissue and the surrounding normal tissue. MMP14 gene expression was not different in different stages in laryngeal glottic cancers, but correlated to the differentiation and lymph node metastasis (P < 0.05). There was correlation between MMP14 gene expression and the stage, differentiation and lymph node metastasis in the laryngeal supraglottic cancers (P < .05). MMP14 protein was localized predominantly in the carcinoma cell cytoplasm and in the stromal fibroblast cytoplasm, and weakly or not expressed in surrounding normal tissue. MMP14 protein expression was much higher in tumor tissue than that in surrounding normal tissue in most of the cases. In general, MMP14 protein expression was the same as MMP14 gene expression and related to the stage, differentiation and lymph node metastasis in the laryngeal cancers. There was no survival difference at 3, 5 and 7-year between the group with higher MMP14 protein expression in tumor tissues than surrounding normal tissues and the group with no difference of MMP14 protein expression (Log Rank, P=0.5535). CONCLUSIONS: The protein MMP14 may play role in laryngeal cancer invasion in a certain extent, but important role in lymph node metastasis of laryngeal cancer. The over-expression of MMP14 protein may be a marker for lymph node metastasis of laryngeal cancer.
[Treatment of pediatric obstructive sleep apnea hypopnea syndrome].
Cai XL, Liu HY, Fan XL … +1 more , Wang TC
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563088
OBJECTIVE: To evaluate treatment options of pediatric obstructive sleep apnea hypopnea syndrome (OSAHS) and their curative effect. METHODS: In this study, there are 59 confirmed pediatric obstructive sleep apnea hypopnea...
OBJECTIVE: To evaluate treatment options of pediatric obstructive sleep apnea hypopnea syndrome (OSAHS) and their curative effect. METHODS: In this study, there are 59 confirmed pediatric obstructive sleep apnea hypopnea syndrome cases diagnosed by polysomnography (PSG). Among them, 54 cases were treated by tonsillectomy or/and adenoidectomy directed by transoral endoscopy. Continue positive airway pressure (CPAP) was used for 2 cases in long-term. Three cases were treated with other conservative therapy. OSA-18 (quality of life for children with obstructive sleep apnea 18 items) was adopted as a disease-specific quality-of-life follow-up survey before and after treatment. RESULTS: During perioperation period, no postoperative bleeding or acute respiratory obstruction occurred. Follow-up sleep study was carried out 12-18 months postoperatively, no complications of velopharyngeal insufficiency, nasopharyngeal stenosis or residual adenoid were found. OSA-18 scores showed that quality of life had been significant improved after operation in 76.3% (45 cases), sleep disturbance in 88.1% (52 cases) and physical symptoms in 67.8% (40 cases). The pressure level of long-term CPAP in 2 cases was about 5.6-7.8 cmH2O. 3 cases were slightly improved treated with conservative therapy. CONCLUSIONS: Surgical removal of upper airway obstruction caused by enlargement of tonsil and adenoid is one of the most effective treatment for pediatric OSAHS. Fibrous endoscopy and cephalometric analysis are helpful to confirm surgical indication. The advantages of transoral endoscopy directed adenoidectomy are as follows: clear and direct visualization, complete removal, without damaging of the peripheral structures.
[Olfactory function and olfactory marker protein in patients with chronic sinusitis].
Gao HY, Zhu XN, Peng SD … +4 more , Zhang HH, Fan EZ, Wang H, Han DM
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563087
OBJECTIVE: To study the pathogenesis of dysosphresia in patients with chronic sinusitis, to discuss the expression and significance of olfaction marker protein in olfactory mucosa. METHODS: There were 55 cases with chron...
OBJECTIVE: To study the pathogenesis of dysosphresia in patients with chronic sinusitis, to discuss the expression and significance of olfaction marker protein in olfactory mucosa. METHODS: There were 55 cases with chronic sinusitis in the experimental group, and 11 cases in the control. All of them were first tested by subjective method, and then the olfactory marker protein in olfactory mucosa was examined by the method of immunohistochemistry. Tissue sections were observed under optical microscope. RESULTS: The ratio of dysosphresia in patients with chronic sinusitis was 50.9% (28/55), while by subjective olfactory test, it was 85.5% (47/55). The ratio of dysosphresia in control patients was 9.09% (1/11), by subjective olfactory test, it was 18.2% (2/11). Olfactory marker protein was identified in the olfactory cells and microvillar cells by histochemical and immunohistochemical technique (S-P method). CONCLUSIONS: The main cause of dysosphresia in patients with chronic sinusitis is the decrease, atrophy and degeneration of the olfactory cells, which lead to the failure of olfactus perception and recognition.
[Expression and DNA binding activity of NF-kappaB in mucosal tissue of chronic rhinosinusitis].
Li TL, Li Y, Zhang GH … +2 more , Sun YN, Li YQ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563086
OBJECTIVE: To study the expression and activation of nuclear factor-kappa B (NF-kappaB) in mucosa of rhinosinusitis. METHODS: The expressions of NF-kappaB p50 and p65 in the mucosa from 18 patients and 10 norms were dete...
OBJECTIVE: To study the expression and activation of nuclear factor-kappa B (NF-kappaB) in mucosa of rhinosinusitis. METHODS: The expressions of NF-kappaB p50 and p65 in the mucosa from 18 patients and 10 norms were detected with the method of immunohistochemistry. The activation of DNA-binding proteins which was labelled with 32P-radiolabeled oligonucleotide probe for NF-kappaB was detected with electrophoretic mobility shift assays (EMSA) in mucosa. RESULTS: Plasma of epithelial and glandular cells displayed a strongly positive staining reaction to p65, and nucleus displayed a strongly positive staining reaction to p50 (7.8%-52.1%). There was significantly difference (chi2 = 22.917, P < 0.01). The DNA-binding proteins activity of 18 samples from patients with chronic rhinosinusitis (28.14 +/- 16.71) was stronger than that (9.28 +/- 2.84) in normal subjects (t = 4.56, P < 0.05). CONCLUSIONS: The expression and DNA-binding proteins activity of NF-kappaB was enhanced. It indicated that NF-kappaB was activated in mucosal inflammation of chronic rhinosinusitis.
[Inferior vestibular nerve impairment in auditory neuropathy].
Wu ZM, Zhou N, Zhang SZ … +6 more , Wang QJ, Guo WW, Gu R, Yu LM, Yang WY, Han DY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563085
OBJECTIVE: To investigate if auditory neuropathy have inferior vestibular nerve (IVN) lesion and to explore the relation between AN and the IVN lesion by vestibular evoked myogenic potentials (VEMPs). METHODS: VEMPs were...
OBJECTIVE: To investigate if auditory neuropathy have inferior vestibular nerve (IVN) lesion and to explore the relation between AN and the IVN lesion by vestibular evoked myogenic potentials (VEMPs). METHODS: VEMPs were observed in 13 patients with auditory neuropathy. And the relation among the duration, hearing threshold of lower frequency and speech discrimination score with VEMPs were observed. RESULTS: Fifty-four percent patients in auditory neuropathy had abnormal VEMPs. They took the form of lower amplitude and no response. The statistical analysis showed that the abnormality of VEMPs had no correlation with lower frequency hearing loss, the duration and speech discrimination score. CONCLUSIONS: The IVN dysfunction may coexist with auditory neuropathy, having lesion in the IVN. However, there was no significant relation between the severity of AN and VEMPs, which meant that AN and inferior vestibular neuropathy had their independence to some extent.
[Characteristics of the response of vestibular evoked myogenic potentials to different stimulus modes].
Zhou N, Wu ZM, Zhang SZ … +4 more , Guo WW, Yu LM, Yang WY, Han DY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563084
OBJECTIVE: Vestibular evoked myogenic potential (VEMP) has been utilized in clinic to test the function of saccule. In the present study, 3 stimulus modes were applied to 21 normal adults and intended to draw in a clinic...
OBJECTIVE: Vestibular evoked myogenic potential (VEMP) has been utilized in clinic to test the function of saccule. In the present study, 3 stimulus modes were applied to 21 normal adults and intended to draw in a clinical test protocol. METHODS: The normal latency and amplitude of VEMPs of 21 normal subjects were recorded and the ratio of bilateral amplitudes and asymmetry were calculated. RESULTS: The response to binaural clicks were similar to the response of the monaural clicks. The interindividual variation in amplitude were large, but the latency varied little. CONCLUSIONS: VEMPs is a stable myogenic potentials. Monaural and binaural clicks stimulus can be used in clinical practice. The latter is appropriate for normal hearing subjects. Amplitude measurement is probable to evaluate the asymmetry of bilateral saccular function and may reveal saccule lesion.
[Relationship between GATA-3 and IL-4 and IL-5 in patients with allergic rhinitis].
Li HB, Han DM, Zhou B … +1 more , Liu ZY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563083
OBJECTIVE: To evaluate the relationship between GATA-3 and IL-4, IL-5 in patients with allergic rhinitis. METHODS: The expression of GATA-3 was detected by immunochemistry and reverse transcriptase polymerase chain react...
OBJECTIVE: To evaluate the relationship between GATA-3 and IL-4, IL-5 in patients with allergic rhinitis. METHODS: The expression of GATA-3 was detected by immunochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) in 23 patients with allergic rhinitis. IL-4, IL-5 were detected by enzyme-linked immunosorbent assay (ELISA). RESULTS: The positive rate of GATA-3 was 86.9% (20/23) and the relative density ratio of GATA-3 and glyceraldehyde phosphate dehydrogenase (GAPDH) were 0.602 +/- 0.11 in patients with allergic rhinitis. The concentrations of IL-4, IL-5 were (135.5 +/- 66.4) pg/mg, (77.5 +/- 29.4) pg/mg, respectively. The expressions of GATA-3 and IL-4, IL-5 had positive relationship (r = 0.45 and 0.62, P < 0.05). CONCLUSIONS: GATA-3 contributed to the production of IL-4, IL-5 in patients with allergic rhinitis.
[Expression of aquaporin-1 and Bcl-2 mRNA in eosinophils of the nasal polyps and its significance].
Guan GM, Dong Z, Lü M
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563082
OBJECTIVE: To study the significance of aquaporin-1 (AQP-1) expression in the eosinophils of nasal polyps. The expression and location of AQP-1 mRNA and apoptosis associated gene Bcl-2 mRNA in nasal polyps were explored....
OBJECTIVE: To study the significance of aquaporin-1 (AQP-1) expression in the eosinophils of nasal polyps. The expression and location of AQP-1 mRNA and apoptosis associated gene Bcl-2 mRNA in nasal polyps were explored. METHODS: Sixteen nasal polyp samples were collected from 11 women and 5 men aged 20-65 years during routine endonasal surgery. Nasal mucosa specimens from the inferior turbinates of 10 patients with allergic rhinitis (7 women and 3 men, aged 16-58 years), collected during septoplasty, were used as controls. The expression of AQP-1 mRNA and Bcl-2 mRNA was detected in serial adjacent sections by in situ hybridization and eosinophils were examined by stain MGG. RESULTS: AQP-1 mRNA expression was found in all 16 nasal polyps and in 4 of 10 inferior turbinate tissues, the mean expression rates were (93.16 +/- 13.25)% and (19.54 +/- 4.98)%, respectively. All 16 nasal polyps and 10 control nasal tissues expressed Bcl-2 mRNA, by the average rates of (84.74 +/- 12.10)% and (16.45 +/- 3.12)%, respectively. The expression of AQP-1 mRNA was positively correlated with Bcl-2 mRNA expression in nasal polyps (r = 0.875, P < 0.01). CONCLUSIONS: AQP-1 contributes to the survival of eosinophils in nasal polyps by keeping the permeation balance of eosinophils.
[Study of sensitivity to impulse noise in guinea pig, rat and mouse].
Qiu JX, Fang XZ, Tong BS … +2 more , Laurell G, Duan ML
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563081
OBJECTIVE: To study sensitivity to impulse noise in guinea pig, rat and mice. METHODS: Six groups were divided. Group I: Guinea pigs (n = 5) were exposed to 50 impulses of 160 dB SPL; Group II: Guinea pigs (n = 5) were e...
OBJECTIVE: To study sensitivity to impulse noise in guinea pig, rat and mice. METHODS: Six groups were divided. Group I: Guinea pigs (n = 5) were exposed to 50 impulses of 160 dB SPL; Group II: Guinea pigs (n = 5) were exposed to 100 impulses of 160 dB SPL; Group III: Guinea pigs (n = 5) were exposed to 200 impulses of 160 dB SPL; Group IV: Guinea pigs (n = 6) were exposed 400 impulses of 160 dB SPL; Group V: Rats (n = 10) were exposed to 50 impulses of 160 dB SPL; Group VI: Mice (n = 10) were exposed to 50 impulses of 160 dB SPL. ABR thresholds were determined prior to, immediately, one day, one week, two weeks and four weeks after impulse noise exposure. RESULTS: The rat and mice were shown temporary threshold shift (TTS) and permanent threshold shift (PTS) after 50 times impulse noise (160), while no TTS and PTS in the guinea pigs but it was shown TTS and PTS after 400 times impulse noise (160). CONCLUSIONS: Sensitivity to impulse noise is different among guinea pig, rat and mouse. Guinea pig has less sensitivity to impulse noise while rat and mouse have higher sensitivity to impulse noise. In addition, rat has higher sensitivity to impulse noise than mouse.
[Connection of efferent neurons and afferent vestibular nuclei in rat].
Jiao Y, Chi FL
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563080
OBJECTIVE: To study the connection between the efferent and afferent vestibular neurons in the center neural system. METHODS: In the present study, a retrograde neuron tracer horseradish peroxidase (HRP) was injected int...
OBJECTIVE: To study the connection between the efferent and afferent vestibular neurons in the center neural system. METHODS: In the present study, a retrograde neuron tracer horseradish peroxidase (HRP) was injected into the surrounding of efferent vestibular neurons located in the inner side of the facial genu of the rat and after following 48 hours for the retrograde axonal transport of this tracer, then its presence in neurons of the brain stem was demonstrated histochemically. RESULTS: Neurons labeled with HRP were found bilaterally in both Medial vestibular nuclei (MVe) and lateral vestibular nuclei (LVe). Approximately 80% of the neurons in each were located in the MVe on the side ipsilateral to the injection. CONCLUSIONS: There is a direct connection between the efferent and afferent vestibular neurons. These findings suggest that there is an afferent-efferent feedback regulatory mechanism in the vestibular system.
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