Zhonghua Er Bi Yan Hou Ke Za Zhi[JOURNAL]
Sun
200 papers
[Nerve exploration and decompression for traumatic recurrent laryngeal nerve injuries induced by thyroid gland surgery].
Chen SC, Zheng HL, Zhou SM … +7 more , Li ZJ, Huang YD, Zhang SQ, Shen XH, Wen W, Liu F, Chen G
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563079
OBJECTIVE: To show the findings of recurrent laryngeal nerve injury exploration and find out therapeutic effects, indications and timing of nerve decompression for traumatic recurrent laryngeal nerve injury induced by th...
OBJECTIVE: To show the findings of recurrent laryngeal nerve injury exploration and find out therapeutic effects, indications and timing of nerve decompression for traumatic recurrent laryngeal nerve injury induced by thyroid gland surgery. METHODS: In this study there were 87 patients with recurrent laryngeal nerve injury, including 65 for nerve exploration and 22 for nonsurgical treatment. During nerve exploration, the types, severity of laryngeal nerve injuries and laryngeal muscular mass were studied. Nerve decompression was performed in these 14 patients whose compressing sutures or compression due to cicatricial hypertrophy were received nerve decompression. RESULTS: Injuries caused by thyroid gland operations mostly are of suture ligation (43%) and nerve severance (48%); simple scar compression was found only in 6 cases (9%). Atrophy of the laryngeal muscles was not very serious in patients with a course less than 6 months. In 10 patients with a course less than three months, nerve decompression restored normal functional abductor and abductor motion of the vocal cord in 9 patients and had no effects in one. Although functional motion of vocal cord was not seen in one case with a course less than 3 months and 4 cases between 3 and 5 months, the mass and tension of the reinnervated vocal cord became much the same as the contralateral normal vocal cord, thus resuming symmetric vibration of the vocal cords and physiological phonation. Although nonsurgical treatment improved severe hoarseness, it didn't restore normal functional motion of the vocal cord and normal voice. CONCLUSIONS: Nerve exploration showed a primary rule for recurrent laryngeal nerve injury induced by thyroid gland surgery. Early and mid-stage recurrent laryngeal nerve exploration and decompression may restore normal motion of the glottis, and it suggested laryngeal delayed reinnervation may help patients with a course more than 6 months.
[Surgical treatment of thyroid carcinoma with the upper mediastinal invasion].
Ge JH, Zhao RL, Hu JL … +1 more , Zhou WA
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563078
OBJECTIVE: To study the surgical management of the thyroid carcinoma with the upper mediastinal invasion. METHODS: Among the 560 thyroid carcinoma cases receiving surgery from 1988 to 1999, there were 10 cases of the upp...
OBJECTIVE: To study the surgical management of the thyroid carcinoma with the upper mediastinal invasion. METHODS: Among the 560 thyroid carcinoma cases receiving surgery from 1988 to 1999, there were 10 cases of the upper mediastinal, the 10 cases were retrospectively analyzed for their preoperative diagnosis, surgical methods and postoperative complications. RESULTS: The upper mediastinal invasion rate of the 560 cases was 1.9% (10/516). There are three paths for the upper mediastinal invasion: (1) Trachea esophagus groove and upper mediastinal lymphatic node metastasis. (2) Tumor direct invasion. (3) Primary malignant substernal goiter. Operative methods include: (1) Tumor removal without using sternum incision. (2) Tumor removal with sternotomy. (3) Extension operation with sternum or/and clavicle bones removal. The number of those cases who survived 1, 3, 5, 10 years were 10, 8, 6, 4 respectively. Radical removal of tumors was achieved in 9 cases. The complications occurred were: 2 cases of chylous fistula; 2 cases of vocal paralysis; 1 case of thyrocervical trunk artery bleeding; 1 case of phrenic nerve paralysis; 1 case of pneumothorax. CONCLUSIONS: Surgery can be adopted for treating thyroid carcinoma with the upper mediastinal invasion, and it can have a good long-term prognosis.
[Endoscopic thyroid gland surgery without carbon dioxide neck insufflation].
Huang XM, Zheng YQ, Xu G … +6 more , Cai X, Gong J, Liu X, Peng JR, Xu YD, Liu W
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563077
OBJECTIVE: To evaluate endoscopic thyroid gland surgery without carbon dioxide neck insufflation. METHODS: Sixteen patients with thyroid gland benign tumor, aged from 23-62 years, were selected and treated with endoscopi...
OBJECTIVE: To evaluate endoscopic thyroid gland surgery without carbon dioxide neck insufflation. METHODS: Sixteen patients with thyroid gland benign tumor, aged from 23-62 years, were selected and treated with endoscopic surgery. Eight cases received whole thyroid lobectomy and the other eight cases received partial thyroid lobectomy, the procedure was through a wall method or a single 2.0-3.0 cm horizontal skin incision. Among the 16 cases, 6 were thyroid adenoma and 10 were nodular goiter. RESULTS: All patients received endoscopic surgery, 2 cases with sub-clavicle approach and 14 cases with a single 2.0-3.0 cm horizontal skin incision approach. The recurrent laryngeal nerve and parathyroid glands were easily identified and preserved during operation, the operating time lasted from 1.1 hours to 4.0 hours, no complication occurred, the postoperative cosmetic outcome was excellent. CONCLUSIONS: The results indicated that gasless technique is feasible and safe, so it suggested that the video-assisted thyroid surgery without carbon dioxide neck insufflation could be an alternative for thyroid gland surgery.
[Re-operation for thyroid carcinoma patients who received nonstandard operation].
Song M, Chen FJ, Wu GH … +3 more , Yang AK, Chen WK, Ou-yang D
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563076
OBJECTIVE: To analyze the reasons for re-operation and living condition of thyroid carcinoma patients who received second operation, and to formulate indications for selecting re-operation candidates and operation types....
OBJECTIVE: To analyze the reasons for re-operation and living condition of thyroid carcinoma patients who received second operation, and to formulate indications for selecting re-operation candidates and operation types. METHODS: One hundred and eleven thyroid carcinoma patients were retrospectively summarized. All patients received re-operation after thyroidectomy from 1986 to 1997. The post operative follow-up data were analyzed statistically. RESULTS: Pathological results confirmed there were 67.6% (75/111) cases with residual tumor. The incidence rate of post-operative complications was 2.7%, including 0.9% (1/111) recurrent laryngeal nerve injury. The sensitivity of residual carcinoma detection by CT scan before operations was 80.0% (28/35), with the positive predictive value of 87.5% (28/32). The recurrence rate within 3 years was 11.7% (13/111), with a local control rate of 88.3% (98/111). The recurrence rate within 5 years was 14.4% (16/111), with a local control rate of 85.6% (95/111). Life table was adopted to calculate living rates. The accumulated 5-year and 10-year living rates of re-operated thyroid carcinoma patients were 95.0% and 93.2% respectively. Analysis of Cox Regression showed that carcinoma recurrence influenced survival conditions significantly. CONCLUSIONS: The residual rate of re-operated patients with thyroid carcinoma was relatively higher, so a cautious re-operation was necessary. CT scan has a relatively higher positive predictive rate in detecting residual carcinoma, so it is necessary for screening right candidates for re-operation. Recurrence was the main factor that influenced patients' survival. So making proper indications, according to which to select patients who need re-operations will decrease the incidence of unnecessary operations.
[Several surgical therapeutical problems of the thyroid carcinoma].
Li SC
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Aug · PMID 15563075
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[Measurement of respiratory ciliary beat frequency quantified with high-speed digital microscopy].
Zhang L, Han DM, Wang H … +2 more , Zhou B, Sanderson MJ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469118
OBJECTIVE: To accurately quantify the respiratory cilia activity, a high-speed phase-contrast imaging and ciliary beat frequency (CBF) analysis method were introduced. METHODS: Airway ciliated cells, from rabbit trachea,...
OBJECTIVE: To accurately quantify the respiratory cilia activity, a high-speed phase-contrast imaging and ciliary beat frequency (CBF) analysis method were introduced. METHODS: Airway ciliated cells, from rabbit trachea, mouse trachea and human nose, were prepared by primary culture of airway epithelial cells. Phase-contrast images were detected with a digital high-speed camera, a progressive scan charge-coupled device(CCD) that provided images (648 pixels x 200 lines) at 240 frames per second (fps). A data analysis approach, which can measure the period [frequency (Hz) = 1/period] of each ciliary beat cycle to match the high temporal resolution of the image acquisition rate, was also introduced. RESULTS: The high signal-to-noise ratio of gray waveform was obtained by interactively selecting the optimal region of interest. The frequency of each ciliary beat cycle was determined from the period of each cycle of the gray -intensity waveform. At 30 degrees C, the basal CBF (x +/- s(x-)), from mouse, rabbit and human, were (15.6 +/- 0.7) Hz (n = 5), (13.2 +/- 0.9) Hz (n = 7), and (13.4 +/- 1.1) Hz (n = 5), respectively. No statistical difference was found among groups (P > 0.05). In response to extracellular ATP, CBF had a rapid increment that could occur in seconds, a behavior suggesting the necessity of the high temporary resolution data acquisition system. CONCLUSIONS: This ease of recording and replaying high-speed images may be useful in analyzing different phases and forms of ciliary beat patterns and perhaps in the diagnosis of cilia dysfunction-related diseases.
[Comparison of prevalence rates of secretory otitis media in Hong Kong Chinese children with western populations].
Tong M, Yue V, Ku PK … +2 more , Lo PS, Van Hasselt CA
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469117
OBJECTIVE: To investigate the prevalence rate of secretory otitis media (SOM) in Hong Kong Chinese children and further compare the results with the western studies. METHODS: From 1995 to 1998, primary schools, kindergar...
OBJECTIVE: To investigate the prevalence rate of secretory otitis media (SOM) in Hong Kong Chinese children and further compare the results with the western studies. METHODS: From 1995 to 1998, primary schools, kindergartens and nurseries were selected by stratified randomization in Hong Kong of China. Six thousand eight hundred and seventy-two children of age 2 to 7 were examined on-site in the school premises by the otolaryngologist and audiologist with otoscope and tympanometry respectively. In order to achieve standardization comparisons, our raw data were retrieved and the prevalence rates were recalculated according to the various diagnostic criteria set by the western studies. RESULTS: The prevalence of SOM in the age-groups 2-3, 4-5 and 6-7 of the present study ranged from 5.2% to 21.6% if criteria were set to clinical otoscopic findings and ranged from 7.3% to 30.7% if criteria were based on tympanometric findings. The variations in the diagnostic criteria with tympanometric findings also lead to a deviation of prevalence rates with the same set of data. Regardless of the criteria set for investigations, there are no significant differences between our findings and those of the western studies with the same age groups. Prevalence rates decrease as age increases. CONCLUSIONS: The prevalence rates of SOM in Chinese children of age 2-3, 4-5 and 6-7 in Hong Kong are not significantly different from those reported in the literature of the West.
[Application of 99mTc(V)-dimercaptosuccinic acid imaging in detecting neck metastases in patients with oral squamous cell carcinoma].
Zhang Y, Guo CB, Yu GY … +3 more , Ma DQ, Li Q, Zhang CL
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469116
OBJECTIVE: To evaluate the applicability of 99mTc(V) -dimercaptosuccinic acid (99mTc (V) -DMSA) imaging in detecting neck metastases in patients with oral squamous cell carcinoma. METHODS: Single photon emission computer...
OBJECTIVE: To evaluate the applicability of 99mTc(V) -dimercaptosuccinic acid (99mTc (V) -DMSA) imaging in detecting neck metastases in patients with oral squamous cell carcinoma. METHODS: Single photon emission computer tomography (SPECT) scintigraphy with 99mTc(V) -DMSA was performed on 32 patients with primary squamous cell carcinoma in oral cavity. The coronal aspect of SPECT image was chosen, the region of interest (ROI) of the affected neck was drawn, and the radioactivity uptake value of the ROI was given by the computer to compare with the contralateral neck. The results of 99mTc(V) -DMSA were compared with postoperative pathological studies. For false positive lymph nodes, semi-continuous slice was carried out for pathology examination. RESULTS: The sensitivity, specificity and accuracy of 99mTc(V) -DMSA imaging for detecting metastatic lymph nodes were 75.0%, 90.0% and 84.4% respectively. The ROI values of the affected neck are higher than that of the contralateral part in the 9 positive patients (t = 8.128, P = 0.000 04). Metastasis was not found in the enlarged lymph nodes of the false positive patients. CONCLUSIONS: 99mTc(V) -DMSA SPECT imaging has fairly high affinity with metastatic lymph nodes in the neck especially in the cN0 patients and is helpful for designing proper neck dissection.
[Reconstruction of hypopharyngeal circumferential defects: pharyngogastric anastomosis or free jejunal interposition].
Zhang B, Tang PZ, Xu ZG … +3 more , Qi YF, Li DZ, Zhang Z
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469115
OBJECTIVE: To choose the optimal reconstruction for circumferential defects of the hypopharynx between pharyngogastric anastomosis and free jejunal interposition is. METHODS: Retrospective review of the archives of 125 p...
OBJECTIVE: To choose the optimal reconstruction for circumferential defects of the hypopharynx between pharyngogastric anastomosis and free jejunal interposition is. METHODS: Retrospective review of the archives of 125 patients who underwent pharyngoesophageal reconstruction with pharyngogastric anastomosis(n = 92) or free jejunal interposition(n = 33). Analysis was confined to patient with advanced hypopharyngeal cancer or recurrent laryngeal cancer who had hypopharyngeal circumferential defects after tumor ablation. RESULTS: The morbidity and mortality associated with reconstructive procedures were significantly higher in the pharyngogastric anastomosis group than in the free jejunal interposition group (43% versus 21%, P = 0.023 and 11% versus 0%, P = 0. 048). The risk factors related to complications associated with the procedures were reconstruction with pharyngogastric anastomosis (OR 2.97; 95% CI 1.14; 7.76) and albumin < 40.0 g/L(OR 2.87; 95% CI 1.33; 6.16) . The occurrence of swallow obstruction or regurgitation was higher in the pharyngogastric anastomosis group than in the free jejunal interposition group(76% versus 12%, P = 0.00). Patients in the pharyngogastric anastomosis group had lost weight of 3.3 kg (95% CI - 5.7; - 1.0) postoperatively, on the contrary, patients in the free jejunal interposition group had gained weight of 2.8 kg(95% CI 0.9; 4.7) postoperatively. CONCLUSION: Patients reconstructed with free jejunal interposition had lower mortality and complications than with pharyngogastric anastomosis. Furthermore, the former seems to have better quality of life than the latter. The first choice of reconstructive strategy for hypopharyngeal circumferential defects is free jejunal interposition.
[Non-recurrent laryngeal nerve during thyroid surgery: a report of 4 cases and literature review].
Li XY, Lu XS, Wang ZM … +2 more , Huang JH, Li JD
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469114
OBJECTIVE: To investigate clinical significance of non-recurrent laryngeal nerve. METHODS: Clinical data from 4 cases of non-recurrent laryngeal nerve and related literature review was made to acknowledge its incidence,...
OBJECTIVE: To investigate clinical significance of non-recurrent laryngeal nerve. METHODS: Clinical data from 4 cases of non-recurrent laryngeal nerve and related literature review was made to acknowledge its incidence, variant types and matters concerned during thyroid surgery. RESULTS: Seven hundred and nineteen recurrent laryngeal nerves were exposed during 2156 thyroid operations in Xiangya Hospital, from which 4 were confirmed to hold non-recurrent laryngeal nerves (0.56%). Two cases were found on the right side and the other 2 on the left. Among the 4 cases, 3 (patients) were found to have non-recurrent laryngeal nerves during reoperation because of voice horse after the first operation. The other one was recognized during the first operation. CONCLUSIONS: Non-recurrent laryngeal nerve, a rare anomaly, is very vulnerable during thyroid surgery. Knowing the related knowledge of the non-recurrent laryngeal nerve and its types is helpful to avoid its damage during thyroid surgery.
[Comprehensive prognostic value of spontaneous and evoked electromyography in laryngeal paralysis].
Chen SC, Zheng HL, Zhou SM … +5 more , Li ZJ, Zhang SQ, Huang YD, Wen W, Cui Y
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469113
OBJECTIVE: To determine the comprehensive prognostic value of spontaneous and evoked electromyography (EMG) in laryngeal paralysis. METHODS: The characteristics of laryngeal EMG of 91 cases with unilateral vocal cord par...
OBJECTIVE: To determine the comprehensive prognostic value of spontaneous and evoked electromyography (EMG) in laryngeal paralysis. METHODS: The characteristics of laryngeal EMG of 91 cases with unilateral vocal cord paralysis (VCP) after thyroid surgery were assessed. All cases were divided into four groups according to the interval of laryngeal EMG after onset, which were group one (2 months shorter, n = 13), group two (2 to 4 months, n = 23), group three (4 to 6 months, n = 36), group four (6 months longer, n = 19). The waveform morphology and the amplitude of laryngeal EMG and the highest evoked compound muscular active potential (CMAP) of thyroarytenoid muscles were examined and analyzed during voluntary tasks. The potential amplitude was showed by the percentage of that of the healthy lateral. The criterion of evaluation on evoked potential was attained by calculating statistical confidence interval. RESULTS: The highest evoked CMAP in group one was significantly lower than that of the others (P < 0.05), but no significant difference was observed between group two and group three (P > 0.05), so group two and group three were analyzed together. There were 2 recovered cases and 11 unrecovered cases in group one. On the basis of this criterion that a positive prognosis for laryngeal recovery was indicated when the evoked CMAP presented and there was no misdirect generated potential, correct prognostic rate was 92% (12/13). There were 11 recovered cases and 48 unrecovered cases in group two and three. The highest evoked CMAP was much higher in the recovered than in the unrecovered, significant difference was observed between them (P < 0.001). On the basis of the criterion that a positive prognosis for laryngeal recovery was indicated when the highest evoked CMAP was higher than 26. 4%, correct prognostic rate was 90% (53/59). On the basis of the criterion that a positive prognosis for laryngeal recovery was indicated when there was no misdirect generated potential and the highest evoked CMAP was higher than 26.4%, correct prognostic rate was 93% (55/59). When the interval from onset to laryngeal EMG recovering was longer than 6 months, none of these patients had return of vocal cord mobility whatever were the outcomes of laryngeal EMG. CONCLUSIONS: Correct prognostic rate can be improved if the prognosis of VCP in different courses is judged respectively by analyzing comprehensively spontaneous and evoked EMG.
[Use of septo-rhinoplasty in the treatment of traumatic deviated nose].
Wang PH, Wu QW, Sun YY … +2 more , Shi RJ, Tang JY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469112
OBJECTIVE: To explore the new classification and marking method for traumatic deviated nose treated by septo-rhinoplasty. METHODS: Twenty-six selected cases of traumatic deviated nose were analysed. There were 5 C-type c...
OBJECTIVE: To explore the new classification and marking method for traumatic deviated nose treated by septo-rhinoplasty. METHODS: Twenty-six selected cases of traumatic deviated nose were analysed. There were 5 C-type cases, 12 O-type cases and 9 S-type cases. Deviated parameters were measured before and after operation. All patients were treated by seven-step method. RESULTS: Clinical data in seventeen patients including C-type and O-type were complete. There was significant difference in changes of deviated parameters before and after operation( t = 6.9031, P = 0.0001). The cure rate was 58.8%, the effective rate was 88.2%. CONCLUSIONS: The new clinical classification and marking method for traumatic deviated nose are suitable for clinical study. Septo-rhinoplasty is effective for traumatic deviated nose.
[Observation of maxillary mucosa restoration after the endoscopic sinus surgery operation of chronic sinusitis and nasal polyps].
Tong YF, Sun XZ, Li DW
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469111
OBJECTIVE: To understand the restoration process of the mucosa through the continual endoscopic, microscopic, transmission electron microscopic and scanning electron microscopic observation of the nasal mucosa after endo...
OBJECTIVE: To understand the restoration process of the mucosa through the continual endoscopic, microscopic, transmission electron microscopic and scanning electron microscopic observation of the nasal mucosa after endoscopic sinus surgery (ESS). This restoration process of mucosa can be used to appraise the effectiveness of the operation. METHODS: Thirty-one cases (53 sides) with chronic nasal sinusitis and nasal polyps were selected randomly, which had been undergone ESS operation during January to December, 2001. Biopsy of posterior frontanelle of the maxillary sinus were performed to observe the mucosa changes before the ESS and at 2-3 weeks, 8-11 weeks and 13-16 weeks after the ESS, respectively. RESULTS: Epithelia shedding, proliferation, squamous metaplasia, glandular and fibrous tissue proliferation and lymphocytes infiltration were observed in all cases before the ESS. The microtubule abnormal and mitochondria diminishing and expanding existed in all cases. The morphological changes were not significant within 2-3 weeks after the ESS compared to the changes before the ESS. Eight-eleven weeks after the ESS, the nasal cavity turned clean and smooth. The number of pillar cells increased and the cilia swelling, short cilia, and many pillar cells with microvillus were seen. The number of goblet cells increased. Pathological glandular and fibrous proliferation were seen in all cases (53 sides). The operated cavity was clean and covered completely by epithelia (50 sides) 13-16 weeks after ESS. The length of cilia increased and arrangement of the cilia line was in the same direction. The structure of microtubules was clear. The mitochondria was elongated and became dense (49 sides). The pathological repairing was at work (3 sides). CONCLUSIONS: After the ESS, the recovery of the mucus needs 3 months. Some pathological changes are irreversible and the cilia pathological change is related with its restoration. The more cilia reserved and timely local cleaned after ESS, the more helpful to the restoration of the cilia morphology and function. The effective of ESS can be evaluated through the histological-morphological changes. timely local cleaned after ESS, the more helpful to the restoration of the cilia morphology and function. The effective of ESS can be evaluated through the histological-morphological changes.
[Largest extension of CO2 laser surgery for laryngeal cancer in experimental animals].
Huang ZG, Han DM, Yu ZK … +2 more , Ni X, Chen XH
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469110
OBJECTIVE: To explore the security and limit resection extension of CO2 laser surgery for laryngeal cancer in experimental animal under self-retaining laryngoscope. METHODS: Ten experimental dogs were selected and receiv...
OBJECTIVE: To explore the security and limit resection extension of CO2 laser surgery for laryngeal cancer in experimental animal under self-retaining laryngoscope. METHODS: Ten experimental dogs were selected and received CO2 laser surgery with self-retaining laryngoscope. Vertical partial or supraglottic horizontal laryngectomy was performed according to the surgical criteria. All the dogs were killed immediately (5/10) or 40 days later(5/10) by using air embolus postoperatively. The whole larynx was taken out, and specimens were embedded with colloidion and then serially sectioned. Dimension of excision and wound surface recovery status were observed. RESULTS: All the operations on experiment animals were successful and the results were satisfactory. The excision dimension was the same as standard surgery. Gross specimens and serial sectioning staining of 5 dogs were performed. And the other 5 dogs wound surface had already recovered. CONCLUSIONS: CO2 laser surgery was comparable with traditional vertical partial laryngectomy and supraglottic horizontal laryngectomy for excision size. Animals were able to survive the surgery, wound surface could recover spontaneously. It suggested the possibility of extended laryngectomy with laser.
[Endostatin in the treatment of the transplantable model of human laryngeal squamous carcinoma in nude mice].
Yao HC, Jin DJ, Sun YN … +2 more , Ren MH, Li XD
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469109
OBJECTIVE: To evaluate the inhibitory effect of endostatin on tumor growth of human laryngeal squamous carcinoma in nude mice and to explore the possible mechanism of the inhibition and the possible way of biological the...
OBJECTIVE: To evaluate the inhibitory effect of endostatin on tumor growth of human laryngeal squamous carcinoma in nude mice and to explore the possible mechanism of the inhibition and the possible way of biological therapy. METHODS: Nude mice model bearing laryngocarcinoma was established by using human laryngeal squamous carcinoma cell line ( Hep-II). The animals were given endostatin (20 mg x kg(-1) x d(-1)) or PBS, for 21 consecutive days. The volumes of the subcutaneous tumor were observed. The microstructure in which the general 2-step immuohistochemical examination was adopted and ultra-microstructural changes of carcinoma after administration of endostatin were observed under light and electron microscopes for pathology examination. RESULTS: The differences were statistically significant for the net mice weight, tumor weight, and tumor volume and weight/net mice weight between the treatment group and the control group. The restrained percentage of tumor was 45.9%. The necrosis and apoptosis of the tumor cell and the angiogenesis reduction were found under light and electron microscope in the treatment group. The expression of MVD, PCNA and VEGF of the treatment group is lower than that of the control group, and T test showed that P < 0.01, P < 0.05, P < 0.05 respectively, the differences were statistically significant. CONCLUSIONS: These studies showed that endostatin could significantly restrain the development of laryngocarcinoma. The mechanism may be due to the effect of antiangiogesis.
[Research of the electrophysiological properties of acutely dissociated spiral ganglion neurons isolated from mice using patch clamp technique].
Deng JH, Fan JG, Liang CY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469108
OBJECTIVE: To explore the electrophysiological properties of spiral ganglion neurons (SGN) isolated from neonatal mice. METHODS: Ion channel currents of acutely trypsin-dissociated spiral ganglion neurons was recorded an...
OBJECTIVE: To explore the electrophysiological properties of spiral ganglion neurons (SGN) isolated from neonatal mice. METHODS: Ion channel currents of acutely trypsin-dissociated spiral ganglion neurons was recorded and analyzed using whole-cell variation of the voltage clamp technique. RESULTS: In the membrane of SGN, we recorded tetraethylammonium (TEA)-sensitive and 4-aminopyridine (4-AP)-sensitive outside delayed rectifier potassium currents, A-type potassium currents and tetrodotoxin-sensitive inside sodium currents. Ion channel activity had the character of voltage-dependence. Some cells, which did not exhibit sodium currents exhibited, delayed rectifier potassium currents. CONCLUSIONS: The electrophysiological properties of acutely dissociated spiral ganglion neurons could be reference to the research of the mechanics of auditory propagation and the ion channel pharmacology.
[Subunit of voltage-dependent calcium channels in the murine spiral ganglion cells].
Peng H, Xie DH, Xiao ZA … +3 more , Wu WJ, Chen Y, Xia K
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jul · PMID 15469107
OBJECTIVE: To investigate subunit type of voltage-dependent calcium channels in the spiral ganglion cells of the mouse. METHODS: The spiral ganglion cells were dissected from cochleae of neonatal mice and cultured for 24...
OBJECTIVE: To investigate subunit type of voltage-dependent calcium channels in the spiral ganglion cells of the mouse. METHODS: The spiral ganglion cells were dissected from cochleae of neonatal mice and cultured for 24 h. Total RNA was extracted from cultured spiral ganglion cells. After reverse transcription, resulting cDNA was amplified by polymerase chain reaction (PCR) with primers targeted to nucleotide sequences corresponding to 7 different calcium channel subunits. The types of calcium channel subunits were identified by PCR analysis and nucleotide sequencing. RESULTS: Reverse transcription (RT)-PCR products representing subunit gene expression were strongly and consistently amplified for alpha1 D, alpha1 E, alpha2/delta, beta1 and beta3. Nucleotide sequencing confirmed the identity of mouse cochlear subunit cDNAs. CONCLUSIONS: alpha1D, alpha1E, alpha2/delta, beta1 and beta3 subunits are expressed in spiral ganglion cells. And the coexpression of alpha1D and alpha1 E demonstrate the presence of L-type and R-type calcium channels in mammalian spiral ganglion cells.
[Visualization and thin sectional anatomy of the laryngeal cartilages].
Tan LW, Zhang SX, Song L … +6 more , Liu ZJ, Qiu MG, Li QY, Li K, Wang YS, Tang ZS
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469085
OBJECTIVE: To establish digitized visible model of the laryngeal cartilages of the visible human, providing morphological data for image diagnosis and laryngectomy. METHODS: Cross-sectional images of fresh tissues from t...
OBJECTIVE: To establish digitized visible model of the laryngeal cartilages of the visible human, providing morphological data for image diagnosis and laryngectomy. METHODS: Cross-sectional images of fresh tissues from the Chinese visible human data set were reviewed, and the laryngeal cartilages structures data were used, the data was on a section-by-section basis. Three-dimensional computer reconstructions of the laryngeal cartilages were generated from these data by surface rendering on a SGI workstation. RESULTS: The digital images from the visible human offer unique insights into the complex anatomy and ossification of the laryngeal cartilages, the quality of the computerized 3D-reconstructed images was distinct and perfect. CONCLUSIONS: The visible human data set can provide complete and accurate data The digitized model of the laryngeal cartilages offer unique insights into the laryngeal anatomy, could be used for resident education, rehearsal of an unfamiliar surgery and for developing a new surgical approach.
[Investigation and analysis of quality of life for patients after laryngectomy].
Su ZZ, Xian ZX, Chai LP … +2 more , Jiang AY, Luo FT
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469084
OBJECTIVE: To institute and test the quality of life for laryngectomy, and evaluate the feasibility of above questionnaire in patients after laryngectomy. METHODS: A questionnaire of quality of life for laryngectomy was...
OBJECTIVE: To institute and test the quality of life for laryngectomy, and evaluate the feasibility of above questionnaire in patients after laryngectomy. METHODS: A questionnaire of quality of life for laryngectomy was instituted. An investigation was conducted in patients treated by partial (36, Group A) or total (45, Group B) laryngectomy for laryngeal cancer with above questionnaire. The feasibility, reliability and validity of the questionnaire was evaluated, the quality of life and the influencing factors in laryngectomy were also analyzed. RESULTS: The questionnaire of quality of life for laryngectomy consists of 22 items, covering six aspects. The questionnaire has a comparatively good reliability, and the criterion-related validity and content validity of the questionnaire is valid. The correlation coefficient of split-half method and Cronback's alpha of questionnaire were 0.842 and 0.889 respectively. The composite quality of life scores of group A were significant higher than those of group B ( P < 0.001). The differences in physical function, laryngeal function, psychological state, the ability of living independently were statistically significant (P < 0.001; P < 0.001; P < 0.01; P < 0.05) as well. Seven factors including operative modality, tumor stage, postoperative complication, coexisted disease, family incomes, voicing modality and wearing tracheo-cannula were related to postlaryngectomy quality of life. CONCLUSIONS: The questionnaire of quality of life for laryngectomy has speciality in laryngectomy and a comparatively good reliability and validity, and it is suitable for quality of life research in patients after laryngectomy.
[Evaluation of sentinel lymph node in squamous cell carcinoma of the head and neck].
Liu MB, Qi YF, Tang PZ … +5 more , Xu ZG, Chen MQ, Liu SY, Yin YL, Liu WS
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469083
OBJECTIVE: To assess the feasibility of sentinel lymph node (SLN) radiolocalization in stage in head and neck squamous cell carcinoma and to gain insight as to whether the sentinel lymph node could be prognostic of regio...
OBJECTIVE: To assess the feasibility of sentinel lymph node (SLN) radiolocalization in stage in head and neck squamous cell carcinoma and to gain insight as to whether the sentinel lymph node could be prognostic of regional micrometastatic disease. METHODS: A prospective trial was made on the application sentinel lymph node radiolocalization in 10 patients with NO squamous cell carcinoma of the head and neck region. For each patient a peritumoral submucosal injection of filtered technetium 99m prepared with dextran colloid (99mTc-DX) was performed immediately. After 30 minutes, focal areas of accumulation corresponding to sentinel lymph nodes (SLN) were marked on the skin surface. The SLN was localized by lymphoscintigraphy and intraoperatively through the intact skin by a hand-held gamma-probe. SLN was defined as the count of lymph node could be detected 4 times more than that of background. Complete neck dissections were performed, and SLNs were identified for later histological evaluation and comparison to the remaining lymphadenectomy specimen. RESULTS: SLN radiolocalization accurately identified one or more SLNs in the ten cases. 3 of 10 cases revealed occult metastatic disease. Therefore the negative metastasis rate was 30% (3/10). The positive rate of SLN and nonsentinel lymph node (NSLN) were 22.7% (5/22) and 0.4% (1/247) respectively. Serial sectioning (SS) and immunohistochemical (IHC) on SLNs would reduce the false-negative rate of sentinel lymph node biopsy (SLNB). There was no instance in which SLN was negative for micrometastatic disease while being positive in a nonsentinel lymph node. CONCLUSIONS: SLN evaluation in NO neck in squamous cell carcinoma of the head and neck is accurately feasible and seems to adequately predict the presence of occult metastasis. Although SLN radiolocalization in head and neck squamous cell carcinoma may potentially reduce the time, cost, and morbidity of regional lymph node management, more experience with technique is required before its role can be determined.
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