Zhonghua Er Bi Yan Hou Ke Za Zhi[JOURNAL]
Sun
200 papers
[Expression of survivin gene and its relationship with expression of p15, p16 proteins in laryngeal squamous cell carcinomas].
Zhu J, Xu RJ, Wu ZH … +4 more , Yan CL, Cao XL, Fang Y, Lin L
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469082
OBJECTIVE: : To investigate the expression of Survivin (SVV) and its relationship with expression of p15, p16 in laryngeal squamous cell carcinoma (LSCC). METHODS: Using strep avidin-biotin complex (SABC) method, we exam...
OBJECTIVE: : To investigate the expression of Survivin (SVV) and its relationship with expression of p15, p16 in laryngeal squamous cell carcinoma (LSCC). METHODS: Using strep avidin-biotin complex (SABC) method, we examined the expression of SVV, p15 and p16 gene in 48 LSCC tissus samples, 24 normal laryngeal mucosa tissus samples and 24 normal laryngeal tissus adjacent to the tumors samples. RESULTS: SVV was expression in 27 of 48 (56.3%) samples of LSCC and expression in 6 of 24 (25.0%) samples of normal laryngeal tissus adjacent to the tumors. Normal laryngeal mocosa tissus samples did not expressed SVV. Overexpression of SVV was related to the tumor site, grade, clinical stage and tumor prognosis (P < 0.05). The expression of SVV in LSCC was positive correlated with p16 expression( C = 0.52 P < 0.001), but not with p15 expression. CONCLUSION: SVV may play a role in the pathway of carcinogenesis and tumor progress. It is feasible for early diagnosis and prognosis estimation. Overexpression of SVV gene and de-activation of antioncogene p16 may be play synergetic roles in the carcinogenesis of LSCC.
[The influence of protein kinase C inhibitor in eosinophil apoptosis of nasal polyps].
Li MH, Yang ZQ, Yin WZ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469081
OBJECTIVE: To study the influence of the inhibitor of protein kinase C (PKC), Iso-H7 [1-(5-lsoquinolinyl-sulfony)-3-methylpiperazine dihydrochloride] on eosinophil apoptosis in nasal polyp tissues. METHODS: Nasal polyp t...
OBJECTIVE: To study the influence of the inhibitor of protein kinase C (PKC), Iso-H7 [1-(5-lsoquinolinyl-sulfony)-3-methylpiperazine dihydrochloride] on eosinophil apoptosis in nasal polyp tissues. METHODS: Nasal polyp tissues were cultured and divided into two groups, test group (with Iso-H7 in different concentration) and control group (without the inhibitor of PKC), to check out the influence of Iso-H7 on eosinophil apoptosis in nasal polyps. The nasal polyp tissues were cultured in Iso-H7 in concentration of 10(-6), 10(-4), 10(-2) mol/L for 1 d, 3 d, and 5 d, respectively. Eosinophil apoptosis index was measured with TUNEL (terminal-deoxynucleotidyl transferase d-UTP nick end labeling), in situ hybridization and MGG staining to identify eosinophil apoptosis. RESULTS: Occasionally, there was little eosinophil apoptosis in two groups in 1 d, but in 3 d, There were a great deal of eosinophil apoptosis in test group. The eosinophil apoptosis indexs of the two groups were significantly different (P < 0.01). The indexs of eosinophil apoptosis was not significantly higher in 3 d than that in 5 d in test groups (P > 0. 05), but significantly higher than that in 1d (P < 0. 01). CONCLUSIONS: Iso-H7 takes a important role in accelerating eosinophil apoptosis. The inhibitor of PKC could significantly promote eosinophil apoptosis in nasal polyps. There is no dosage and time dependence of PKC inhibitor in regulating eosinophil apoptosis of nasal polyps.
[Computer-assisted anatomical study of nasofrontal region].
Yang QT, Shi JB, Kang Z … +4 more , Chen HX, Wang T, Lü JT, Xu G
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469080
OBJECTIVE: To evaluate the value of Advantage Windows 3.1 (AW 3.1) software for anatomical study of nasofrontal region, and to study the CT characteristics of nasofrontal region which related to the frontal sinus surgery...
OBJECTIVE: To evaluate the value of Advantage Windows 3.1 (AW 3.1) software for anatomical study of nasofrontal region, and to study the CT characteristics of nasofrontal region which related to the frontal sinus surgery. METHODS: Eighty patients underwent axial consecutive computed tomography scans and these data were studied with AW 3.1 software which provided reconstructional imaging of continuous coronal, sagittal, axial sections. Some related structures of nasofrontal region were studied and measured. RESULTS: AW 3.1 software could identify and measure the following structures accurately: The diameter of frontal sinus was (22.5 +/- 8.6) mm in height, (16.3 +/- 6.8) mm in depth, (23.8 +/- 9.8) mm in breadth. The diameter of frontal sinus ostium: the anterior-posterior diameter was (7.3 +/- 1.7) mm, the transverse diameter was (8.5 +/- 1.9) mm. The width of nasal beak of frontal bone(5.9 +/- 1.4 ) mm. The distance of frontal sinus ostium to the floor of columella nasi and the corresponding angle to the nasal floor were (60.8 +/- 4.2) mm and (70.1 +/- 4.7) degrees. The superior attachment sites of the uncinate process were as follows: lamina papyracea 41%, posteromedial wall of agger nasi cell 11%, middle turbinate 19%, anterior skull base 16%, superior bifurcation 13%. The cells could impinge on the frontal recess to cause obstruction (terminal recess 38.8%, anterior ethmoid cell 27.6%, agger nasi cells 24.5%). The accessory cells could impinge on the frontal sinus (perifrontal cells 32.7%, superaorbital cells 38.8%, intersinus septal cells 32.0%). There was significant difference between two groups of characteristics of nasofrontal region. CONCLUSIONS: AW 3.1 software is a helpful and powerful new tool for anatomical study of nasofrontal region and for preoperative evaluation. The structures of nasofrontal region are complex and various, frontal sinusitis almost always results from the obstruction of frontal sinus outflow tract. These results of anatomical study of nasofrontal region are helpful in directing the functional endoscopic surgery in frontal sinus.
[Study of the relation between Cx31 gene and hereditary hearing impairment].
Gao WH, Ke XM, Liu YH … +2 more , Zhu P, Pan KF
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469079
OBJECTIVE: To study the relation between hereditary nonsyndromic hearing impairment (NSHI) in Chinese and mutation in Connexin 31 (Cx31) gene and to explore the pathogenic mechanism. METHODS: Forty-seven pedigrees with h...
OBJECTIVE: To study the relation between hereditary nonsyndromic hearing impairment (NSHI) in Chinese and mutation in Connexin 31 (Cx31) gene and to explore the pathogenic mechanism. METHODS: Forty-seven pedigrees with hereditary NSHI, 38 Children with sporadic NSHI and cases of control were collected in present studies. The coding sequence of Cx31 gene was amplified by polymerase chain reaction (PCR), screened by denaturing high-performance liquid chromatography (DHPLC) and confirmed by direct sequencing. RESULTS: The mutation rate of heterozygous mutation C --> T at position 798 of Cx31 cDNA in patient group and in control were 14.1% (12/85) and 1% (1/100) respectively. Significant difference was found between the two group (P < 0.01). Heterozygous mutation G --> A at position 580 of GJB3 cDNA, which results in a missense mutation (A194T), was found in two members of one pedigree with autosomal dominant NSHI. The mutation was not found in numbers with normal hearing of this pedigree and controls. Heterozygous mutation G --> A at position 250 of Cx31 cDNA was found in one child with sporadic congenital NSHI. In our previous studies, Cx26 gene mutations have been screened among the patient with hereditary NSHI and sporadic NSHI and the control of our test, and two Cx26 gene mutations were found in two pedigrees. But the two NSHI pedigrees which were confirmed to have Cx26 gene mutation were not found to have Cx31 mutation. The patient and the control which were confirmed to have Cx31 gene mutations were not found to have Cx26 mutations. CONCLUSIONS: Cx31 gene was associated with nonsyndromic hearing impairment There was no cross and cooperative effect between Cx26 gene and Cx31 gene.
[Repair of the rabbit ear cartilage defects with transforming growth factor-beta1 and allogenic chondrocyte/poly-DL-lactide higher porosity polymer].
Li JH, Huang JZ, Du J … +1 more , Cheng Y
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469078
OBJECTIVE: To study the repair of the rabbit ear cartilage defects with transforming growth factor-beta1 (TGF-beta1) and allogenic chondrocyte/poly-DL-lactide (PDLLA) higher porosity polymer. METHODS: A total of 18 rabbi...
OBJECTIVE: To study the repair of the rabbit ear cartilage defects with transforming growth factor-beta1 (TGF-beta1) and allogenic chondrocyte/poly-DL-lactide (PDLLA) higher porosity polymer. METHODS: A total of 18 rabbits were divided into the TGF-beta1 and chondrocytes/PDLLA graft group, the chondrocytes/PDLLA graft group, and the blank control group. Samples were taken out at 4, 12 and 18 weeks after implantation. The histological characteristics were investigated. RESULTS: After 18 weeks of the transplantation, the repaired effects of TGF-beta1 and allogenic chondrocytes/PDLLA graft group was better than the allogenic chondrocytes/PDLLA graft group not only on general sample but also histology, and the blank control group were repaired with fibrous tissue. CONCLUSIONS: The allogenic chondrocytes/PDLLA graft could repair the rabbit ear cartilage defects while TGF-beta1 could improve the quality of the rabbit ear cartilage defects.
[Analysis of 60 masses in the nasal vestibule].
Zhou SH, Xu YY, Wang SQ … +3 more , Ling L, Yao HT, Ren GP
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469077
OBJECTIVE: To summarize the treatment strategy of nasal vestibule masses. METHODS: Between May, 1982 and April,2003,60 patients with nasal vestibule mass were treated in our department. The clinical features of these mas...
OBJECTIVE: To summarize the treatment strategy of nasal vestibule masses. METHODS: Between May, 1982 and April,2003,60 patients with nasal vestibule mass were treated in our department. The clinical features of these masses were retrospectively analysed. RESULTS: Among 60 masses, 51 cases were benign tumors, 5 cases were inflammatory masses, and 4 cases were malignant tumors. All patients were surgically treated and discharged with complete recovery. Among patients with benign tumor and non-specific inflammatory mass, 37 patients with a median follow-up of 97 months without recurrence and malignant change. One patient with hidradenoma and one patient with rhinoscleroma recurred and another surgery was carried out. There was no recurrence after second surgery. 6 patients died of other diseases, and 11 patients lost follow-up. Two patients with malignant melanoma recurred after first surgery. Then the tumor was removed via lateral rhinotomy and died of local recurrence and liver metastasis. Two patients (one with papilloma carcinomatous change and another with non-Hodgkin lymphoma) were free of diseases 17 years or 5 years and 2 months respectively. CONCLUSIONS: Masses of different histological types can be found in the nasal vestibule. Surgical removal is the treatment of choice. The approaches are determined on the basis of site, size and kind of the masses.
[Rescue operations of the recurrent nasal and paranasal sinus neoplasms].
Zhang YP, Lin L, Gong LF … +1 more , Huang DL
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469076
OBJECTIVE: To investigate the symptoms, factors relating to recurrences, the styles of the rescue operation and the reconstructive methods of the surgical defects in patients with recurrent nasal and paranasal sinus neop...
OBJECTIVE: To investigate the symptoms, factors relating to recurrences, the styles of the rescue operation and the reconstructive methods of the surgical defects in patients with recurrent nasal and paranasal sinus neoplasms. METHODS: Excluding the primary neoplasm, only patients with recurrent tumors and suitable for rescue operations entered the group. Kaplan-Meier method was used to estimate the survival probability. RESULTS: Twenty-five patients receiving rescue operations from 1993 to 2002 who met the above inclusion criteria were reviewed. The age ranged from 13 - 66 years. All the patients had once surgery or radiation therapy at least. The time between the last treatments and the recurrences ranged from 2 weeks to 46 months. 80 per cent of the recurrences were within the first 2 years after the last therapy. The recurrent symptoms included headache (10 patients), eminences in or around the operation fields (9 patients), diminution of vision (7 patients), and so on. The patients were followed 1 to 65 months. There were no patients died during the rescue operations. Five patients were alive without recurrences and 3 alive with local or regional recurrences. Thirteen died of local recurrences and 1 died of metastasis to lung. The one-year, 2-year and 3-year survival rate were 62.5%, 43.7% and 29.1%, respectively. The median survival time was 18 months. Complications included cerebrospinal rhinorrhea (3 patients), central diabetes insipidus (1 patient) and necrosis of part of the flap (1 patient). CONCLUSIONS: Most of the local recurrences occur within 2 years after the last therapies. The recurrent symptoms are commonly headache and eminences in or around the operation fields. The recurrences are closely related to pathological types and differential degrees of the neoplasms. The reasonable and timely combined treatment may help to decrease the frequencies of the recurrences. The rescue operations with effective methods can improved the patients' life qualities and lengthen their life spans. The main complications are cerebrospinal rhinorrhea most of which can be cured by conservative treatment.
[Clinical analysis of 34 cases of esthesioneuroblastoma].
Liu WS, Tang PZ, Xu GZ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469075
OBJECTIVE: To study the diagnosis, treatment policy and prognosis in patients with esthesioneuroblastoma. METHODS: A retrospective review was conducted in 34 patients treated at Cancer Hospital of CAMS from 1958 to 1998....
OBJECTIVE: To study the diagnosis, treatment policy and prognosis in patients with esthesioneuroblastoma. METHODS: A retrospective review was conducted in 34 patients treated at Cancer Hospital of CAMS from 1958 to 1998. According to the Kadish system, the patients were staged as follows: stage A, 1 case; stage B, 9 cases; and stage C, 24 cases. Three patients were treated with surgery alone, 9 irradiation alone, and 15 combination with surgery and radiation, 4 combination with radiation and chemotherapy and 3 surgery, radiation and chemotherapy. RESULTS: The overall 5-year survival rate was 47.1% (16/34). The survival rates for stag A, B, and C were 100% (1/1), 88.9% (8/9), and 29.2% (7/24) respectively. The survival rates for surgery alone, radiation alone and combined therapy were 33.3% (1/3), 33.3% (3/9), and 54.5% (7/24), respectively. The local control rate and distant metastasis rate were 61.8% (21/34) and 32.4% (11/34), respectively. There were 10 patients died of distant metastasis and 5 patients died of local recurrence. The survival rate in patients with early stage (A + B) esthesioneuroblastoma was significantly better than that in patients with advanced stage (C) (chi2 = 8. 174, P = 0.004). The young patients (< or = 30 years) had a remarkable higher distant metastasis rate (chi2 = 3.865, P = 0.049) and poor prognosis (chi2 = 4.194, P = 0.041) than elder patients. CONCLUSIONS: Early detection and combined therapy were important to improve survival rate. The combined therapy with preoperative radiation therapy and surgery could help to achieve excellent local control. Distant metastasis was an important factor which affected the prognosis of the patients.
[Expression and correlation of apoptosis-related gene c-IAP2 and caspase-4 in sinonasal squamous carcinoma].
Li H, Zeng ZH, Wang LH … +1 more , Wang JQ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469074
OBJECTIVE: To examine the expression of apoptosis-related genes c-IAP2 and caspase-4 in sinonasal squamous carcinoma. To investigate the role and mechanism of the two apoptosis-related genes in nasal cavity and sinus squ...
OBJECTIVE: To examine the expression of apoptosis-related genes c-IAP2 and caspase-4 in sinonasal squamous carcinoma. To investigate the role and mechanism of the two apoptosis-related genes in nasal cavity and sinus squamons carcinoma. METHODS: Forty-one pathologically confirmed specimens of nasal cavity and sinus squamons carcinoma were subjected for immunohistochemical staining to analyze the expression of c-IAP2 and caspase-4, in addition 10 normal nasal mucosa were used as control. RESULTS: The positive rate of c-IAP2 in sinonasal squamons carcinoma was 87.8%, among which 14 cases were strongly stained(+ + +), 15 cases were stained(+ +), 7 cases were stained (+), only 5 cases were negative. In 10 normal control tissues, no case was stained(+ + +), 2 cases were stained (+ +), 3 cases were stained(+), the other 5 cases were negative. Higher level expression of c-IAP2 protein was detected more often in squamons carcinoma specimens than that in normal nasal mucosa. There was statistically significant difference between the two groups for the staining(P < 0.05). The expression of c-IAP2 was not correlated with clinical staging (P > 0.05), but there were positive correlation with clinical prognosis (P < 0.01) and pathological classification (P < 0.01); The positive rate of caspase-4 in nasal cavity and sinus squamons carcinoma was 58.5%, among which 4 cases were strongly stained (+ + +), 7 cases were stained (+ +), 13 case was stained(+), 17 cases were negative. The expression of caspase-4 protein was more deletion in squamons carcinoma specimens than that in control group, there was statistically significant difference between the two groups for the staining (P < 0.05). The expression of caspase-4 protein was negatively correlated with clinical prognosis (P < 0.01) and pathological classification ( P < 0.01), not correlated with clinical staging(P > 0.05). The expression of c-IAP2 was negativelly correlated with the experssion of caspase-4. CONCLUSIONS: The results indicates that in sinonasal squamous carcinoma, c-IAP2 is activated, while caspase-4 is restrained, the signal transduction pathway of apoptosis is arrested, leading to surviving of tumor cells, this maybe directly associated with the development of sinonasal squamons carcinoma, and with the failure of tumor trentment.
[Endoscopic guided nasal and skull base operation].
Zhang XB
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 Jun · PMID 15469073
Abstract loading — click title to view on PubMed.
[Clinical evaluation of 18-fluorine fluorodeoxyglucose positron emission tomography-CT imaging in head and neck tumors].
Li XP, Liang Y, Liu X … +2 more , Wang QS, Wu HB
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338869
OBJECTIVE: To study the clinical value of 18-fluorine fluorodeoxyglucose (18 F-FDG) positron emission tomography (PET)-CT imaging in diagnosis and detection of primary tumors and suspicious recurrence in head and neck ca...
OBJECTIVE: To study the clinical value of 18-fluorine fluorodeoxyglucose (18 F-FDG) positron emission tomography (PET)-CT imaging in diagnosis and detection of primary tumors and suspicious recurrence in head and neck cancers. METHODS: Twenty-seven patients with primary tumors or metastases in head and neck underwent 18F-FDG PET-CT imaging, and compared with clinical data. RESULTS: 1. In 4 NPC diagnosed pre-radiation patients the 18F-FDG PET-CT imaging in nasopharynx was all positive, 2 of them were detected with neck lymph node metastases, which were confirmed later histopathologically. In 12 NPC cases after radiation, 4 were not found any evidence of tumor with PET-CT imaging and clinically, 4 were detected neck recurrence and confirmed histopathologically, 2 had remote metastases confirmed by other imaging, 2 were detected and confirmed recurrence in primary sites and one of them in regional and remote site. 2. In 4 neck metastatic patients with unknown primary tumor, the primary tumors were all identified, 3 of them were confirmed histopathologically. 3. Seven patients with other head and neck tumors after therapy underwent 18F-FDG PET-CT imaging. Two patients had no evidence of recurrence. Five patients were detected recurrence or metastases, 4 of them were confirmed histopathologically, one of them refused biopsy. CONCLUSIONS: With metabolic image of PET and anatomic image of CT, 18F-FDG PET-CT is a kind of very useful way in diagnosing primary tumor and detecting the recurrence and metastases in patients with head and neck tumor.
[A study of the relationship between the expression of connexin43, E-cadherin and biological behaviors of human laryngeal cancer].
Sun WH, Liu HM, Li YJ … +2 more , Ji XR, Liang DP
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338868
OBJECTIVE: To investigate expression of connexin43 (Cx43) and E-cadherin (E-cad) and its relationship with the biological behavior in Laryngeal carcinomas. METHODS: Immunohistochemistry staining (PicTure two steps method...
OBJECTIVE: To investigate expression of connexin43 (Cx43) and E-cadherin (E-cad) and its relationship with the biological behavior in Laryngeal carcinomas. METHODS: Immunohistochemistry staining (PicTure two steps method) was used to detect connexin 43 and E-cadherin expression in 60 paraffin-embedded laryngeal carcinomas and 10 normal epithelia around tumors. All the patients were followed up more than five years. RESULTS: Cx43 and E-cad were expressed by normal epithelia with typical membranous staining 10 normal epithelia strongly expressed Cx43 and E-cad. The reduction rate of Cx43 expression in laryngeal carcinomas was 30.0%. The significant relationship was observed between low Cx43 expression and differentiation, lymph node metastasis and recurrence (chi2 = 15.09, 6.41, 3.86, P < 0.01, 0.05, 0.05), but there was no significance between low Cx43 expression and prognosis (chi2 = 2.65, P > 0.05). 41.7% laryngeal carcinoma showed reduced or no expression of E-cad. Tumor cell dedifferentiation correlated with reduced expression for E-cad (chi2 = 15.07, P < 0.01). Absent or low E-cad expression was observed more frequently in patients with local recurrence and lymph node metastasis and with less than 5-year survival period (chi2 = 5.35, 6.65, 5.14; P < 0.05, 0.01, 0.05). There was a positive relationship between the expression of Cx43 and E-cad in same sample (r = 0.63, P < 0.0001). CONCLUSIONS: Laryngeal cancer presented inactivation of Cx43 gene and E-cad gene and down regulation of Cx43 and E-cad proteins. The level of Cx43 and E-cad may be a sensitive predictor of differentiation, invasion, lymph node metastasis and recurrence. E-cad is also a prognostic factor for patients with laryngeal cancer.
[Preliminary study on the functional localization of auditory cortex in the healthy young subjects using magnetic source imaging].
Miao YZ, Sun JL, Bu GQ … +4 more , Rong H, Wu J, Li SM, Liang HR
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338867
OBJECTIVE: To evaluate the value of magnetic source imaging (MSI) in the functional localization of the primary auditory cortex. METHODS: The M100 waves of cortical auditory evoked magnetic fields (AEFs) evoked by 0.5, 1...
OBJECTIVE: To evaluate the value of magnetic source imaging (MSI) in the functional localization of the primary auditory cortex. METHODS: The M100 waves of cortical auditory evoked magnetic fields (AEFs) evoked by 0.5, 1, 2, 4 and 8 kHz pure tones in 5 subjects and by 2 kHz pure tones in 25 healthy young subjects were measured respectively (16 males and 14 females, with the age from 20 to 32 years old) using a whole head 306 channel magnetoencephalography (MEG) system. The MSI obtained by superimposing functional MEG data on structural magnetic resonance image (MRI) was used to localize the M100 sources on the auditory cortex. RESULTS: The M100 waves of AEFs were clear and replicable in both hemispheres. The M100 sources were localized on the bilateral transverse temporal gyri in all 30 subjects. The localization of M100 on transverse temporal gyri varied with the changes of stimulus frequency. The localization of primary auditory cortex was asymmetrical between bilateral hemispheres, and the left hemisphere M100 dipoles were significantly posterior compared to the right M100 dipoles. The M100 responses appeared significantly earlier to the contralateral stimuli than that to the ipsilateral stimuli in both hemispheres. The dipole positions of M100 were independent of the side of the stimuli. CONCLUSIONS: The functional localization of the primary auditory cortex could be determined precisely by magnetic source imaging (MSI) with high spatiotemporal resolution. MSI would hold great promise as a noninvasive tool for the fundamental and clinical research in otology.
[Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth].
Xiao SF, Xi L, Zhou YD … +3 more , Wang CY, Tian PK, Gu JR
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338866
OBJECTIVE: To evaluate the efficiency of marker gene and reporter gene transfection into human squamous cell carcinoma of larynx implanted in nude mice with a new EGFR mediated non-viral polypeptide gene delivery system....
OBJECTIVE: To evaluate the efficiency of marker gene and reporter gene transfection into human squamous cell carcinoma of larynx implanted in nude mice with a new EGFR mediated non-viral polypeptide gene delivery system. METHODS: Labeled streptavidin biotin (LSAB) was applied to examine over-expression of epidermal growth factor receptor (EGFR) on the squamous cell carcinoma of larynx. The complex of marker gene or reporter gene and gene delivery system was prepared and subsequently inoculated with Hep 2 cells or injected into laryngeal carcinoma implanted on nude mice. RESULTS: Over-expression of EGFR was observed on inoculated Hep 2 cells and on 65% of laryngeal carcinoma specimen examined (15/23). Positive staining was located on the surface of the cells and some of them in the cytoplasma. Green fluorescence was observed on Hep 2 cells under fluorescent microscope 48 hrs after PEGFP-C1 gene transfection and became the strongest 72 hrs after transfection. The rate of transfection was 80% (78/97). Blue staining was noticed 12 hrs after beta-gal gene transfer and it became more remarkable 24 hrs after gene transfection with X-gal staining on the implanted human laryngeal carcinoma on nude mice. CONCLUSIONS: Squamous cell carcinoma of head and neck over-expressed EGFR and a non-viral GE7 polypeptide gene delivery system could transfer exogenous genes into laryngeal carcinoma cells and tissues with high efficiency and targeting both in vitro and in vivo suggesting that GE7 would be applicable as a new targeted, high efficient gene delivery system to the gene therapy of squamous cell carcinoma of head and neck.
[Combination radiation and gene therapy for head and neck squamous cell carcinoma in the murine model].
Liu SX, Yang H, Yuan YM … +3 more , Guo YF, Tang ZQ, Liang CY
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338865
OBJECTIVE: To examine the antitumour efficacy and investigate immunological mechanism of combination therapy of IL-2 gene and IL-12 gene transfer with radiation in an immunocompetent murine model that parallel more close...
OBJECTIVE: To examine the antitumour efficacy and investigate immunological mechanism of combination therapy of IL-2 gene and IL-12 gene transfer with radiation in an immunocompetent murine model that parallel more closely the clinical therapy of head and neck squamous cell carcinoma (HNSCC). METHODS: Tumors were established in the floor of mouth in C3H/HeJ mice with SCCVII cell line. Lipid-DNA complexed (lipoplexes) by using polycationic liposome-Mediated transduction for HNSCC was transduced in tumor-bearing mouse by direct intratumoral gene transfer. The local tumor was radiated with a dose of 2 Gy in the second day. Tumor sizes were measured before and after the treatment as compared to the different single treatment groups and the controls. After tumors were subculture, the supernatants were collected for IL-2 and IL-12 expression by enzyme-linked immunosorbent assay (ELISA). Natural killer (NK) cell activity and cytotoxic T-lymphocyte (CTL) activity were also assayed by LDH method. CD4+ and CD8+ T-lymphocyte in tumor tissues were examined by immunohistochemistry. RESULTS: HNSCC tumor growth was significantly inhibited following combined IL-2 and IL-12 gene therapy with radiation as compared to IL-2 or IL-12 gene therapy with radiation, single IL-2 or IL-12 gene therapy, radiation alone and the controls. Increased secreted levels of IL-2 and IL-12 protein expression were found in combined and single IL-2 gene or IL-12 gene treated groups. The combination and single gene treated groups produced greater activation of CTL and NK than the controls of all concerned test. The significant CD4+ and CD8+ T lymphocyte infiltration was distributed and the numerous necrosis were seen in tumor tissues after combination therapy. CONCLUSIONS: Combined IL-2 gene and IL-12 gene therapy with radiation could significantly inhibited HNSCC tumor growth in the murine model and efficiently induced antitumor immunity of the host.
[Expression of aquaporin in rats' endolymphatic sac and kidney and the effect of anti-diuretic hormone on the expression of aquaporin].
Chen T, Zhang R
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338864
OBJECTIVE: To confirm the expression of aquaporin2,3,4 (AQP2,3,4, water channel protein) in rats' endolymphatic sac (ES) and kidney, and to investigate and compare the effects of antidiuretic hormone (AVP) and DDAVP [(de...
OBJECTIVE: To confirm the expression of aquaporin2,3,4 (AQP2,3,4, water channel protein) in rats' endolymphatic sac (ES) and kidney, and to investigate and compare the effects of antidiuretic hormone (AVP) and DDAVP [(deamino-Cys1,D-Arg8)-Vasopressin, V2-receptor agonist] on the expression of AQP2 in rats' ES and kidney. METHODS: Thirty healthy Swards white rats were divided into the negative control, AVP group and dDAVP group, respectively, and were cardiaca perfused. The temporal bones and kidneys were taken out, then processed and sectioned by paraffin-embedded technique. The sections of ES were labeled with fluorescent antibody by immunohistochemical method, and kidney's with avidin-biotin-peroxidase complex method (ABC). The expression of AQP-2,3,4 were confirmed in the ES of rats, and the different effects of the AVP and DDAVP onto the ES and kidney were observed. The slides used were analyzed by image-analyzer and the subsequent data were dealt with statistically. RESULTS: In the cytomembrane and cytoplasm of ES' epithelia, the constant and clear fluorescent reaction could be observed in normal control group with the first antibody of AQP2,3,4. Significant feeble fluorescent reaction of the first antibody of AQP-2 was revealed in AVP group and DDAVP group and showed much lower of gray (P < 0.01), less intensive of fluorescent (P < 0.01) under the fluorescence microscope. In the principal cell of renal collecting duct, it was on the contrary. Compared with the control group, significant stain was revealed in AVP group and DDAVP group and showed lower of gray (P < 0.05), greater density (P < 0.05), higher IOD (P < 0.05) and more stained area (P < 0.05). There is no different between the AVP group and DDAVP group in expression of AQP-2 in two sites. CONCLUSIONS: AQP-2,3,4 were expressed both in rats' epithelia of endolymphatic sac (ES) and principal cell of renal collecting duct. AVP promotes the expression of AQP2 in kidney but inhibits in ES. AVP maybe play important role to control the expression of AQP-2 in ES and kidney probably by the role of AVP-V2R-cAMP-AQP2.
[Round window area temporal bone section versus CT].
Duan JR, Luo HQ, Lu CJ … +3 more , Wei JP, Li MZ, Xiong JP
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338863
OBJECTIVE: To locate round window area and its related structure on auris transection and CT for anatomical evidence of image diagnosis and clinical operation. METHODS: Fifteen normal head specimen fixed with 10% dehyde...
OBJECTIVE: To locate round window area and its related structure on auris transection and CT for anatomical evidence of image diagnosis and clinical operation. METHODS: Fifteen normal head specimen fixed with 10% dehyde were scanned by high-resolution computed tomography on canthomeatal line. CT image (depth 1.00 mm, thick 1.00 mm) was obtained. Temporal bone-centered tissues were taken, decalcified, desiccated and socked with collodion, then embedded and made into sequential transactions (thick 1.00 mm). Lower surface of section was observed by both naked eyes and microscope, then scanned and photographed. Versus CT image, auditory ossicle, osseous semicircular canals, vestibule, round window, niche, cochlea, pyramidal eminence, internal acoustic meatus and cochlear aqueduct were identified respectively. RESULTS: There were 18-22 layers of temporal transection on CT image. Round window and round window niche always appeared on the 10th layer (R) and the 11th layer (L). The mean of the depth of anterior wall was 0.92 mm (R) and 0.90 mm (L), and depth 1.89 mm (R) and 2.04 mm (L). The average distance from niche to jugular fossa wall was 2.10 mm (R) and 2.39 mm (L). No significant difference among of thickness, depth and distance from niche to jugular fossa wall. CONCLUSIONS: Temporal bone transection specimen had a clear picture of anatomical position between round window area and its related structure. Versus CT, the result contributed to image diagnosis and operation on auris diseases.
[CT multiplan reconstruction images of disorder of stapes].
Gong RZ, Chao BT, Liu K … +4 more , Fan ZM, Wang HB, Chen QH, Wu LB
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338862
OBJECTIVE: To evaluate the clinical application of multi-planar reformation (MPR) for the stapes with multi-slice spinal thin-section CT in the patients with disorder of stapes before prosthetic ossicular reconstruction...
OBJECTIVE: To evaluate the clinical application of multi-planar reformation (MPR) for the stapes with multi-slice spinal thin-section CT in the patients with disorder of stapes before prosthetic ossicular reconstruction and their impact on surgical decision. METHODS: Axial CT scanning of temporal bone was undergone in 50 volunteers. Multiplan reformatted images included coronal, sagittal and oblique MPR, were made. All the MPR images were compared with each other in order to show which one could reveal the whole structures of stapes better. The height of stapes was measured with the oblique MPR. CT findings were evaluated in 102 cases with disorder of stapes. Prosthetic ossicular reconstruction was made in 65 cases. RESULTS: The full stapes cannot be shown in axial, coronal and sagittal MPR images, however, it was shown in oblique MPR in all the cases. The highness of stapes was (3.3 +/- 0.4) mm in the abnormal group. There was chronic otitis media in 69 cases (90 ears) and congenital abnormalities of the stapes in 33 cases (47 ears) which included the dispart of incudostapedial joint in 5 cases (7 ears), defect of head and crus of stapes in 9 cases (13 ears), defect of one side of crus in 3 cases (4 ears) and absence of the oval window in 16 cases (23 ears). CONCLUSIONS: Coronal and oblique MPR images from Axial Spiral CT Data are essential for the pre-operative planning of prosthetic ossicular reconstruction. The ship, structure or defect of stapes can be shown in the oblique MPR images. It is important for the patient to choose the type of operation.
[Long-term results of small fenestra stapedectomy compared with large fenestra technique].
Liu ZH, Zhong SX, Yang JH … +3 more , Li XL, Chen J, Zhang M
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338861
OBJECTIVE: To evaluate the postoperative long-term hearing results of small fenestra stapedectomy versus large fenestra techniques. METHODS: A retrospective review of 102 ears of 94 patients who had stapedectomy (small f...
OBJECTIVE: To evaluate the postoperative long-term hearing results of small fenestra stapedectomy versus large fenestra techniques. METHODS: A retrospective review of 102 ears of 94 patients who had stapedectomy (small fenestra technique) performed by the same surgeon between 1972-1990 was conducted. Patients undergoing stapedectomy and stapedotomy were followed for 6.4 +/- 2.2 and 6.6 +/- 3.0 month in initial postoperative and 14.5 +/- 3.0 and 7.4 +/- 2.2 years in late postoperative, respectively. RESULTS: There were no statistically significant differences in initial or late postoperative pure-tone (PTA), PTA air-bone gap, speech reception threshold (SRT) between the two groups. Ears treated by stapedotomy showed statistically better initial postoperative air-conduction threshold and initial 4-KHz air-bone gap closure, but the gap difference was not significant with late follow-up. The successful outcomes in both groups were stable over long-term follow-up. Results were the same when comparing the two procedures in the patients having undergone both. CONCLUSIONS: These results show that, in the operation of an experienced surgeon for otosclerosis, either large or small fenestra technique provides satisfactory and stable long-term results.
[Diagnosis and surgical management of petrous apex cholesteatoma].
Wu T, Han DY, Yang WY … +3 more , Huang DL, Wu WM, Zhang SZ
Zhonghua Er Bi Yan Hou Ke Za Zhi · 2004 May · PMID 15338860
OBJECTIVE: To explore the etiology, diagnosis and surgical management of petrous apex cholesteatoma. METHODS: Twelve cases of petrous apex cholesteatoma (primary 3, secondary 9) were retrospectively studied. RESULTS: Pri...
OBJECTIVE: To explore the etiology, diagnosis and surgical management of petrous apex cholesteatoma. METHODS: Twelve cases of petrous apex cholesteatoma (primary 3, secondary 9) were retrospectively studied. RESULTS: Primary petrous apex cholesteatoma was characterized by non otorrhea history, normal tympanic membrane appearance and the initially occurring symptoms of facial paralysis and hearing loss. In contrast, secondary petrous apex cholesteatoma was characterized by otitis media history, perforated drum, hearing loss and facial paralysis. The treatment principle for either primary or secondary cholesteatoma is to remove all the cholesteatoma. Based on the status of hearing and location and extent of cholesteatoma within temporal bone, 4 surgical approaches were taken in our study, which were translabyrinth, middle cranial fossa, combination of translabyrinth and middle cranial fossa, craniocervical combination approach (intralabyrinth approach). The complications of these surgical procedures and their management were discussed. According to result of 4 months to 15 years follow-up, there were no recurrence cases up to now, except one, which was operated 4 times as recurrence. Facial nerve anastomosis (3/12) or decompression (3/12) was performed simultaneously in the operations. The function of facial nerve partially recovered from V to IV of House and Brackmann grading in 2 out of 3 anastomosis cases and from IV to III in 2 out of 3 cases of decompression. CONCLUSION: Although there are some differences in symptoms and etiology between primary and secondary petrous apex cholesteatoma, complete surgical removal of lesion is necessary for both. Surgical approaches are decided according to location and extent of the lesion and hearing status. Our study indicated that open cavity operation had more advantage in terms of morbidity compared with the closed cavity operation, which closes the auditory canal as a blind sac.
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